CN112501012B - Self-adjusting opening and closing heat cover and full-automatic gene amplification instrument using same - Google Patents
Self-adjusting opening and closing heat cover and full-automatic gene amplification instrument using same Download PDFInfo
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- CN112501012B CN112501012B CN202011408980.0A CN202011408980A CN112501012B CN 112501012 B CN112501012 B CN 112501012B CN 202011408980 A CN202011408980 A CN 202011408980A CN 112501012 B CN112501012 B CN 112501012B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract
The invention discloses a self-adjusting opening and closing thermal cover and a full-automatic gene amplification instrument using the thermal cover. The existing gene amplification instrument adopts a manual uncovering mode, and the provided pressure is different from person to person, so that the gene amplification effect is affected. The invention comprises a driving mechanism and a thermal cover module; the heat cover module comprises a hollow hinged frame, a supporting block, a guide sleeve, a sliding rod, a movable pressing plate, a coil spring, a heat cover shell and an annular rubber pad. The closing process of the thermal cover shell is realized through the action of the spring, and the constant pressing force is ensured to be maintained in the closing process of the thermal cover shell under the action of the spring; the coil springs wound at the two ends can provide constant acting force for the movable pressing plate, so that the pressing force of the movable pressing plate on the PCR tube is constant, 0.1 ml of the PCR tube and 0.2 ml of the PCR tube can be simultaneously applied, the operation is automatically completed, and after the pressing force of the movable pressing plate on the PCR tube is preset, the pressing crack of the PCR tube caused by overpressure can be avoided.
Description
Technical Field
The invention belongs to the technical field of gene amplification instruments, and particularly relates to a self-adjusting opening and closing thermal cover of a universal 0.1 and 0.2 ml PCR tube and a full-automatic gene amplification instrument using the thermal cover.
Background
The gene amplification apparatus is a basic apparatus for polymerase reaction in biomedical field, provides stable and adjustable temperature environment, and has been widely used in industry as DNA fragment amplification apparatus. Most gene amplification instruments basically adopt a manual uncovering mode and a manual knob mode for adjusting the pressure of a thermal cover, so that time and labor are wasted, operability is not flexible enough, different operators have different operation results, gene amplification effects are affected, and even the phenomenon that a PCR tube is cracked due to overlarge pressure occurs; and the universality of the consumable parts of the PCR tube is not strong, basically, only 0.1 ml or 0.2 ml of consumable parts can be used for one model, even if two consumable parts can be used for the model, an auxiliary heightening component is required to be added for realization, and the operation is very troublesome.
Disclosure of Invention
The invention aims at overcoming the defects of the prior art, and provides a self-adjusting opening and closing thermal cover and a full-automatic gene amplification instrument using the thermal cover.
The invention relates to a self-adjusting opening and closing heat cover, which comprises a driving mechanism and a heat cover module; the driving mechanism comprises a stepping motor, a belt transmission mechanism, a rotating shaft, a crank, a sliding block and a connecting rod; the belt transmission mechanism consists of a driving belt pulley, a conveyor belt and a driven belt pulley; the driving belt wheel is fixed with the output shaft of the stepping motor and is connected with the driven belt wheel fixed on the rotating shaft through a conveying belt; one end of the two cranks is respectively fixed with two ends of the rotating shaft; the two cranks are provided with sliding grooves along the length direction and two sliding blocks to form sliding pairs respectively; the two sliding blocks are respectively hinged with the bottom ends of the two connecting rods. The hot cover module comprises a hollow hinged frame, a supporting block, a guide sleeve, a sliding rod, a movable pressing plate, a coil spring, a hot cover shell and an annular rubber pad; two vertical grooves which are symmetrically arranged are formed in two sides of the middle of the hollow hinged frame; the hollow hinged frame is provided with an integrally formed spring column in the vertical groove, and a spring is sleeved on the spring column; the supporting block is arranged in the hollow hinged frame and forms a sliding pair along the height direction of the hollow hinged frame with the heat cover shell fixed outside the hollow hinged frame; the top ends of the two connecting rods of the driving mechanism respectively pass through a corresponding strip groove arranged on two sides of the thermal cover shell and are hinged with two sides of the supporting block respectively through a pin shaft; the two pin shafts respectively penetrate through the vertical grooves on the corresponding sides of the supporting blocks and compress the springs in the vertical grooves on the corresponding sides; the tops of the two pin shafts are respectively provided with a pressing plate, and the pressing plates are fixed with the hollow hinged frame; support rods are fixed at two ends of the support block, and through holes are formed in the two ends of the support block, which are positioned at the support rods; a guide sleeve is fixed at each through hole of the supporting block, and each guide sleeve and one sliding rod form a sliding pair; the top ends of the two sliding rods positioned at one end of the supporting block are respectively fixed with the two ends of one connecting rod, and the top ends of the two sliding rods positioned at the other end of the supporting block are respectively fixed with the two ends of the other connecting rod; the bottom ends of the sliding rods are fixed with a movable pressing plate arranged in the hollow hinged frame; rolling sleeves are sleeved on the two connecting rods; two ends of one coil spring are respectively fixed on the support rod and the rolling sleeve at one end of the support block, two ends of the other coil spring are respectively fixed on the support rod and the rolling sleeve at the other end of the support block, and two ends of each coil spring are respectively wound on the support rod and the rolling sleeve at corresponding positions; the bottom of the heat cover shell is fixed with an annular rubber pad at the outer edge of the movable pressing plate.
Preferably, the supporting block and the supporting rod are fixed through screws.
Preferably, the sliding rod is fixed with the connecting rod through a screw.
Preferably, 96 blind holes or 384 blind holes arranged in an array are formed in the bottom surface of the movable pressing plate.
The full-automatic gene amplification instrument using the self-adjusting opening and closing heat cover comprises a gene amplification module, a rack, a controller and a touch screen; the gene amplification module comprises the self-adjusting heat-opening and closing cover, a deep hole plate placing cavity, a module frame, a heater, a radiator and a temperature sensor; the module frame is supported on a beam fixed with the stand; the heater and the radiator are both fixed on the module frame; the deep hole plate placing cavity is fixed on the heater and the radiator; the temperature sensor is fixed in the deep pore plate placing cavity; the stepping motor base of the self-adjusting heat-opening and closing cover is fixed at the bottom of the module frame, a rotating shaft of the self-adjusting heat-opening and closing cover and the module frame form a revolute pair, and two connecting rods of the self-adjusting heat-opening and closing cover respectively penetrate through corresponding one strip groove formed in two sides of the module frame; one end of the hollow hinged frame of the self-adjusting opening and closing heat cover is hinged with the module frame, and the other end of the hollow hinged frame is freely arranged; the signal wire of the temperature sensor, the signal wire of the heater, the signal wire of the radiator and the signal wire of the stepping motor of the self-adjusting opening and closing heat cover are all connected with the signal wire sockets; the signal wire socket is spliced with a pin row on a pin row led out by the controller, and the controller is connected with the touch screen through the signal wire.
Preferably, the heater is a semiconductor refrigerating plate or a heating pipe.
Preferably, the touch screen is detachably fixed on the frame.
Preferably, two gene amplification modules are provided, wherein the deep hole plate placing cavity of one gene amplification module is suitable for placing a 96-hole deep hole plate, and the deep hole plate placing cavity of the other gene amplification module is suitable for placing a 384-hole deep hole plate.
The invention has the beneficial effects that:
each gene amplification module of the invention independently adopts a mode of automatically opening and closing the thermal cover shell, the closing process of the thermal cover shell drives a crank and a connecting rod through a stepping motor, the connecting rod drives a movable pressing plate, and finally the gene amplification module is realized through the acting force of a spring, and the constant pressing force of the thermal cover shell is ensured to be kept in the closing process under the action of the spring; the coil springs wound at the two ends can provide constant acting force for the movable pressing plate, so that the pressing force of the movable pressing plate on the PCR tube is constant, a user can use two PCR tubes with different specifications of 0.1 milliliter and 0.2 milliliter at will in the test process conveniently, the operation is automatically completed, manual intervention is not needed, and the phenomenon that the PCR tube is cracked due to overlarge pressure can be avoided after the pressing force of the movable pressing plate on the PCR tube is preset; further, when the movable pressing plate is pressed tightly, the annular rubber gasket at the bottom of the shell of the heat cover seals the outer edge position of the deep hole plate, and a constant temperature environment is provided for gene amplification. Furthermore, each gene amplification module can be conveniently replaced and installed in a mode of inserting and pulling row pins and placing or lifting a module frame, so that a customer can conveniently select different gene amplification modules to operate; when a plurality of gene amplification modules are provided, each gene amplification module is independently controlled by a controller.
Drawings
FIG. 1 is an assembled perspective view of the self-adjusting heat cover, deep well plate receiving chamber and module frame of the present invention.
Fig. 2 is a perspective view of the heat cover module of the present invention after removing the heat cover housing.
FIG. 3 is a perspective view showing the structure of the full-automatic gene amplification apparatus of the present invention.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
As shown in fig. 1 and 2, a self-regulating heat-opening and closing cover comprises a driving mechanism and a heat cover module 1; the driving mechanism comprises a stepping motor 2, a belt transmission mechanism 3, a rotating shaft, a crank 4, a sliding block and a connecting rod 5; the belt transmission mechanism 3 consists of a driving belt pulley, a conveyor belt and a driven belt pulley; the driving belt wheel is fixed with the output shaft of the stepping motor 2 and is connected with the driven belt wheel fixed on the rotating shaft through a conveyor belt; one end of the two cranks 4 is respectively fixed with two ends of the rotating shaft; the two cranks are provided with sliding grooves along the length direction and two sliding blocks to form sliding pairs respectively; the two sliding blocks are respectively hinged with the bottom ends of the two connecting rods 5. The heat cover module 1 comprises a hollow hinged frame 6, a supporting block 7, a guide sleeve 8, a sliding rod 9, a movable pressing plate 10, a coil spring 11, a heat cover shell 12 and an annular rubber pad 13; two vertical grooves which are symmetrically arranged are formed on two sides of the middle part of the hollow hinged frame 6; the hollow hinged frame 6 is provided with an integrally formed spring column in the vertical groove, and a spring is sleeved on the spring column; the supporting block 7 is arranged in the hollow hinged frame 6 and forms a sliding pair along the height direction of the hollow hinged frame 6 with the heat cover shell 12 fixed outside the hollow hinged frame 6; the top ends of the two connecting rods 5 of the driving mechanism respectively pass through a corresponding strip groove arranged at two sides of the thermal cover shell 12 and are hinged with two sides of the supporting block 7 respectively through a pin shaft 14; the elongated grooves are formed to avoid interference with the heat cover shell 12 when the connecting rod swings; the two pin shafts respectively penetrate through the vertical grooves on the corresponding sides of the supporting blocks 7 and compress springs in the vertical grooves on the corresponding sides; the tops of the two pin shafts are respectively provided with a pressing plate, and the pressing plates are fixed with the hollow hinged frame 6; support rods are fixed at two ends of the support block 7 (preferably by screws), and through holes are formed in the two ends of the support block 7 located on the support rods; a guide sleeve 8 is fixed at each through hole of the supporting block 7, and each guide sleeve 8 and one slide bar 9 form a sliding pair; the top ends of the two sliding rods 9 positioned at one end of the supporting block 7 are respectively fixed with the two ends of one connecting rod (preferably fixed by screws), and the top ends of the two sliding rods 9 positioned at the other end of the supporting block 7 are respectively fixed with the two ends of the other connecting rod; the bottom ends of the slide bars 9 are fixed with a movable pressing plate 10 arranged in the hollow hinged frame 6; 96 blind holes or 384 blind holes which are arranged in an array are formed in the bottom surface of the movable pressing plate 10; rolling sleeves are sleeved on the two connecting rods; two ends of one coil spring 11 are respectively fixed on a support rod and a rolling sleeve at one end of the support block 7, two ends of the other coil spring 11 are respectively fixed on the support rod and the rolling sleeve at the other end of the support block 7, and two ends of each coil spring are respectively wound on the support rod and the rolling sleeve at corresponding positions; an annular rubber pad 13 is fixed at the outer edge of the movable pressing plate 10 at the bottom of the heat cover shell 12.
As shown in fig. 1, 2 and 3, the full-automatic gene amplification apparatus using the self-adjusting opening and closing thermal cover comprises a gene amplification module 15, a frame 16, a controller and a touch screen 17; the gene amplification module 15 comprises the self-adjusting opening and closing heat cover, a deep hole plate placing cavity, a module frame 18, a heater (a semiconductor refrigerating sheet or a heating pipe can be selected), a radiator and a temperature sensor; the module frame 18 is supported on a beam fixed to the frame 16; the heater and the radiator are both fixed on the module frame; the deep hole plate placing cavity is fixed on the heater and the radiator; the temperature sensor is fixed in the deep hole plate placing cavity; the stepping motor base of the self-adjusting heat-opening and closing cover is fixed at the bottom of the module frame, a rotating shaft of the self-adjusting heat-opening and closing cover and the module frame form a revolute pair, and two connecting rods of the self-adjusting heat-opening and closing cover respectively penetrate through corresponding one strip groove formed in two sides of the module frame; one end of the hollow hinged frame 6 of the self-adjusting opening and closing heat cover is hinged with the module frame, and the other end is freely arranged; the signal wire of the temperature sensor, the signal wire of the heater, the signal wire of the radiator and the signal wire of the stepping motor of the self-adjusting opening and closing heat cover are all connected with the signal wire socket 19; the signal wire socket is spliced with a pin header on a pin header led out by the controller, and the controller is connected with the touch screen 17 through a signal wire; preferably, the touch screen 17 is detachably fixed to the housing 16.
Wherein, one or a plurality of gene amplification modules 15 can be arranged, and when a plurality of gene amplification modules are arranged, the deep hole plate placing cavities of each gene amplification module 15 can be all suitable for placing 96-hole deep hole plates, can be all suitable for placing 384-hole deep hole plates, can be part of the deep hole plates suitable for placing 96-hole deep hole plates, and can be the other part of the deep hole plates suitable for placing 384-hole deep hole plates, so that customers can conveniently select different deep hole plate combination forms to carry out full-automatic gene amplification; as a preferred embodiment, two gene amplification modules 15 are provided, wherein the deep-well plate accommodating cavity of one gene amplification module 15 is suitable for accommodating a 96-well deep-well plate, and the deep-well plate accommodating cavity of the other gene amplification module 15 is suitable for accommodating a 384-well deep-well plate. According to the invention, each gene amplification module 15 can be conveniently replaced and installed in a mode of inserting and pulling row pins and placing or lifting the module frame 18, so that a customer can conveniently select different gene amplification modules 15 to operate; when a plurality of gene amplification modules 15 are provided, each gene amplification module 15 is individually controlled by a controller.
When the full-automatic gene amplification instrument with the self-adjusting opening and closing heat cover is used for gene amplification, firstly, a required gene amplification module 15 is placed on each station beam of a rack 16, and a signal wire socket of each gene amplification module 15 is spliced with a row needle on a row wire led out by a controller; then, placing deep-hole plates with corresponding specifications in deep-hole plate placing cavities of the gene amplification modules 15, and placing PCR tubes at each hole site of the deep-hole plates; then, an instruction is sent to the controller through the touch screen 17, the controller controls the stepping motor 2 of each gene amplification module 15 to rotate, the power of the stepping motor 2 drives the supporting block 7 to move downwards through the belt transmission mechanism 3, the crank 4 and the connecting rod 5, at the moment, the pin shaft 14 applies a pressing force to the spring, and the spring presses the hollow hinged frame 6 and the heat cover shell 12 downwards, so that the supporting block 7 and the heat cover shell 12 swing downwards; when the thermal cover shell 12 is swung down to press the top surface of the deep hole plate placing cavity, and the annular rubber gasket 13 at the bottom of the thermal cover shell 12 seals the position of the top surface of the deep hole plate placing cavity, which is located at the outer edge of the deep hole plate, the supporting block 7 moves downwards relative to the thermal cover shell 12, at the moment, the coil spring 11 pulls the sliding rod 9 to drive the movable pressing plate 10 to move downwards, and all the blind holes at the bottom surface of the movable pressing plate 10 cover all the PCR tubes corresponding to the positions on the deep hole plate. Next, after the temperature and time are set by the touch panel 17, gene amplification is performed.
After the gene amplification is finished, a command is sent to a controller through a touch screen 17, the controller controls a stepping motor 2 of a gene amplification module 15 of a thermal cover shell 12 to rotate, the power of the stepping motor 2 drives a supporting block 7 to move upwards through a belt transmission mechanism 3, a crank 4 and a connecting rod 5, at the moment, a pin shaft 14 applies a pressing force to a pressing plate, and the pressing plate presses a hollow hinged frame 6 and the thermal cover shell 12 upwards, so that the supporting block 7 and the thermal cover shell 12 swing upwards, and the thermal cover shell 12 is far away from the top surface of a deep hole plate placing cavity; in the upward swinging process of the supporting block 7, the coil spring 11 pulls the sliding rod 9 to drive the movable pressing plate 10 to move upwards, so that the movable pressing plate 10 is far away from the deep hole plate.
Each gene amplification module 15 of the invention independently adopts a mode of automatically opening and closing the thermal cover shell 12, the closing process of the thermal cover shell 12 drives a crank and a connecting rod through a stepping motor, the connecting rod drives the movable pressing plate 10, and finally the gene amplification module is realized through the acting force of a spring, and the thermal cover shell 12 is ensured to keep constant pressing force in the closing process under the action of the spring; the coil springs 11 wound at the two ends can provide constant acting force for the movable pressing plate 10, so that the constant pressing force of the movable pressing plate 10 on the PCR tube is ensured, and a user can use two PCR tubes with different specifications of 0.1 milliliter and 0.2 milliliter at will in the test process conveniently, and the operation is automatically completed without manual intervention; further, the movable pressing plate 10 presses the movable pressing plate 10, and meanwhile, an annular rubber pad 13 at the bottom of the thermal cover shell 12 seals the outer edge position of the deep pore plate, so that a constant temperature environment is provided for gene amplification.
Claims (8)
1. The utility model provides a self-interacting heat lid that opens and shuts, includes heat lid module, its characterized in that: the device also comprises a driving mechanism; the driving mechanism comprises a stepping motor, a belt transmission mechanism, a rotating shaft, a crank, a sliding block and a connecting rod; the belt transmission mechanism consists of a driving belt pulley, a conveyor belt and a driven belt pulley; the driving belt wheel is fixed with the output shaft of the stepping motor and is connected with the driven belt wheel fixed on the rotating shaft through a conveying belt; one end of the two cranks is respectively fixed with two ends of the rotating shaft; the two cranks are provided with sliding grooves along the length direction and two sliding blocks to form sliding pairs respectively; the two sliding blocks are respectively hinged with the bottom ends of the two connecting rods; the hot cover module comprises a hollow hinged frame, a supporting block, a guide sleeve, a sliding rod, a movable pressing plate, a coil spring, a hot cover shell and an annular rubber pad; two vertical grooves which are symmetrically arranged are formed in two sides of the middle of the hollow hinged frame; the hollow hinged frame is provided with an integrally formed spring column in the vertical groove, and a spring is sleeved on the spring column; the supporting block is arranged in the hollow hinged frame and forms a sliding pair along the height direction of the hollow hinged frame with the heat cover shell fixed outside the hollow hinged frame; the top ends of the two connecting rods of the driving mechanism respectively pass through a corresponding strip groove arranged on two sides of the thermal cover shell and are hinged with two sides of the supporting block respectively through a pin shaft; the two pin shafts respectively penetrate through the vertical grooves on the corresponding sides of the supporting blocks and compress the springs in the vertical grooves on the corresponding sides; the tops of the two pin shafts are respectively provided with a pressing plate, and the pressing plates are fixed with the hollow hinged frame; support rods are fixed at two ends of the support block, and through holes are formed in the two ends of the support block, which are positioned at the support rods; a guide sleeve is fixed at each through hole of the supporting block, and each guide sleeve and one sliding rod form a sliding pair; the top ends of the two sliding rods positioned at one end of the supporting block are respectively fixed with the two ends of one connecting rod, and the top ends of the two sliding rods positioned at the other end of the supporting block are respectively fixed with the two ends of the other connecting rod; the bottom ends of the sliding rods are fixed with a movable pressing plate arranged in the hollow hinged frame; rolling sleeves are sleeved on the two connecting rods; two ends of one coil spring are respectively fixed on the support rod and the rolling sleeve at one end of the support block, two ends of the other coil spring are respectively fixed on the support rod and the rolling sleeve at the other end of the support block, and two ends of each coil spring are respectively wound on the support rod and the rolling sleeve at corresponding positions; the bottom of the heat cover shell is fixed with an annular rubber pad at the outer edge of the movable pressing plate.
2. A self-regulating, opening and closing thermal cover as recited in claim 1, wherein: the supporting block is fixed with the supporting rod through a screw.
3. A self-regulating, opening and closing thermal cover as recited in claim 1, wherein: the sliding rod is fixed with the connecting rod through a screw.
4. A self-regulating, opening and closing thermal cover as recited in claim 1, wherein: 96 blind holes or 384 blind holes which are arranged in an array are formed in the bottom surface of the movable pressing plate.
5. A fully automatic gene amplification apparatus using a self-regulating heat cover according to any one of claims 1 to 4, characterized in that: the full-automatic gene amplification instrument comprises a gene amplification module, a rack, a controller and a touch screen; the gene amplification module comprises the self-adjusting heat-opening and closing cover, a deep hole plate placing cavity, a module frame, a heater, a radiator and a temperature sensor; the module frame is supported on a beam fixed with the stand; the heater and the radiator are both fixed on the module frame; the deep hole plate placing cavity is fixed on the heater and the radiator; the temperature sensor is fixed in the deep pore plate placing cavity; the stepping motor base of the self-adjusting heat-opening and closing cover is fixed at the bottom of the module frame, a rotating shaft of the self-adjusting heat-opening and closing cover and the module frame form a revolute pair, and two connecting rods of the self-adjusting heat-opening and closing cover respectively penetrate through corresponding one strip groove formed in two sides of the module frame; one end of the hollow hinged frame of the self-adjusting opening and closing heat cover is hinged with the module frame, and the other end of the hollow hinged frame is freely arranged; the signal wire of the temperature sensor, the signal wire of the heater, the signal wire of the radiator and the signal wire of the stepping motor of the self-adjusting opening and closing heat cover are all connected with the signal wire sockets; the signal wire socket is spliced with a pin row on a pin row led out by the controller, and the controller is connected with the touch screen through the signal wire.
6. The fully automatic gene amplification apparatus for self-regulating opening and closing of a thermal cover as set forth in claim 5, wherein: the heater is a semiconductor refrigerating sheet or a heating tube.
7. The fully automatic gene amplification apparatus for self-regulating opening and closing of a thermal cover as set forth in claim 5, wherein: the touch screen is detachably fixed on the frame.
8. The fully automatic gene amplification apparatus for self-regulating opening and closing of a thermal cover as set forth in claim 5, wherein: the gene amplification modules are provided with two, wherein the deep hole plate placing cavity of one gene amplification module is suitable for placing a 96-hole deep hole plate, and the deep hole plate placing cavity of the other gene amplification module is suitable for placing a 384-hole deep hole plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408980.0A CN112501012B (en) | 2020-12-03 | 2020-12-03 | Self-adjusting opening and closing heat cover and full-automatic gene amplification instrument using same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011408980.0A CN112501012B (en) | 2020-12-03 | 2020-12-03 | Self-adjusting opening and closing heat cover and full-automatic gene amplification instrument using same |
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| CN112501012A CN112501012A (en) | 2021-03-16 |
| CN112501012B true CN112501012B (en) | 2023-08-08 |
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| CN202011408980.0A Active CN112501012B (en) | 2020-12-03 | 2020-12-03 | Self-adjusting opening and closing heat cover and full-automatic gene amplification instrument using same |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
| CN113292809A (en) * | 2021-04-14 | 2021-08-24 | 杭州安誉科技有限公司 | Processing technology of high-strength hot cover for gene amplification instrument |
| CN114107034B (en) * | 2021-11-24 | 2024-03-12 | 北京全谱医学检验实验室有限公司 | PCR instrument |
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| CN201080478Y (en) * | 2007-07-03 | 2008-07-02 | 海尔集团公司 | Gene magnification instrument thermal cap compressing apparatus |
| CN202415531U (en) * | 2011-12-31 | 2012-09-05 | 北京东胜创新生物科技有限公司 | Self-regulating gland structure of gene amplification instrument |
| WO2015176675A1 (en) * | 2014-05-21 | 2015-11-26 | Coyote Bioscience Co., Ltd. | Thermal cycler lid configurations and uses thereof |
| CN210596022U (en) * | 2019-07-16 | 2020-05-22 | 杭州米欧仪器有限公司 | PCR instrument |
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| US8247217B2 (en) * | 2008-02-15 | 2012-08-21 | Bio-Rad Laboratories, Inc. | Thermal cycler with self-adjusting lid |
| DE102011011912B4 (en) * | 2011-02-21 | 2020-09-03 | Eppendorf Ag | Laboratory device with lid and press arrangement and method for pressing on |
| DE102018124408A1 (en) * | 2018-10-02 | 2020-04-02 | Biometra GmbH | Device for the thermal treatment of samples |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201080478Y (en) * | 2007-07-03 | 2008-07-02 | 海尔集团公司 | Gene magnification instrument thermal cap compressing apparatus |
| CN202415531U (en) * | 2011-12-31 | 2012-09-05 | 北京东胜创新生物科技有限公司 | Self-regulating gland structure of gene amplification instrument |
| WO2015176675A1 (en) * | 2014-05-21 | 2015-11-26 | Coyote Bioscience Co., Ltd. | Thermal cycler lid configurations and uses thereof |
| CN210596022U (en) * | 2019-07-16 | 2020-05-22 | 杭州米欧仪器有限公司 | PCR instrument |
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