CN112481126B - Novel research and development of coronavirus pneumonia vaccine is with device of cultivateing and separation - Google Patents

Novel research and development of coronavirus pneumonia vaccine is with device of cultivateing and separation Download PDF

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Publication number
CN112481126B
CN112481126B CN202011388069.8A CN202011388069A CN112481126B CN 112481126 B CN112481126 B CN 112481126B CN 202011388069 A CN202011388069 A CN 202011388069A CN 112481126 B CN112481126 B CN 112481126B
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culture dish
cavity
base
culture
mounting
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CN112481126A (en
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安有才
陈晓芬
印露
万杰
游媛媛
戴伟
葛灵睿
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Jiangsu Zhonghui Yuantong Biotechnology Co ltd
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AB&B Bio Tech Co Ltd JS
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/52—Mobile; Means for transporting the apparatus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas

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Abstract

The invention discloses a novel culture and separation device for research and development of coronavirus pneumonia vaccines, which comprises a base, wherein a cavity is fixed on the base and is provided with a sealing cover, and a culture dish is movably arranged in the cavity; the device for culturing and separating the novel coronavirus pneumonia vaccine research and development controls the feeding of the two mounting seats through the clamping screw rod, so that a culture dish is conveniently clamped, and the clamping force of the limiting plate on the culture dish is conveniently controlled by arranging the sliding rod and the clamping spring between the limiting plate and the mounting seats, so that the culture dish is prevented from being damaged; in addition, the culture dish is driven to shake as necessary through the matching of the driving wheel and the return spring, the scraper plate can be driven to slide along the inner wall of the culture dish through the feeding bolt, culture substances are separated from the culture dish conveniently, and discharging is facilitated; through the cooperation of fan and temperature sensor, be convenient for control the temperature in the cavity for the culture dish obtains suitable cultivation condition.

Description

Novel research and development of coronavirus pneumonia vaccine is with device of cultivateing and separation
Technical Field
The invention relates to the technical field of vaccine development, in particular to a novel culture and separation device for research and development of a coronavirus pneumonia vaccine.
Background
Common signs of a person infected with coronavirus include respiratory symptoms, fever, cough, shortness of breath, dyspnea, and the like, and in more severe cases, the infection can cause pneumonia, severe acute respiratory syndrome, renal failure, and even death. Therefore, the development of a new coronary pneumonia vaccine is in intense progress all the time, the vaccine needs to be cultured in the process of developing the vaccine, the existing vaccine development mostly adopts a common culture dish, the position is fixed in the actual use, the adhered culture substance is not convenient to separate from the culture dish, and the inconvenience exists in the use process; therefore, there is a need for improvements in the prior art.
Disclosure of Invention
The invention aims to provide a novel culture and separation device for research and development of coronavirus pneumonia vaccines, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a novel coronavirus pneumonia vaccine research and development is with device of cultivateing and separation, includes the base, be fixed with the cavity on the base, the cavity is equipped with sealed lid, the inside movable mounting of cavity has the culture dish, as the further scheme of this invention: the culture dish is including the upper cover and the lower cover that mutually support, and the upper cover has laid the application of sample groove that can open, and the inboard movable mounting of lower cover has scrapes the flitch, scrapes the lower extreme center department of flitch and rotates and install the feed bolt, and the one end of feed bolt extends to the outside of culture dish, evenly installs a plurality of guide bars between the lower extreme of scraping the flitch and the culture dish, and the guide bar is scalable.
As a further scheme of the invention: the inner side of the cavity is connected with an annular fixed seat, the fixed seat is positioned below the culture dish, a plurality of inclined fans are uniformly arranged on the inner side of the cavity, the fans are positioned above the culture dish in an inclined manner, and a plurality of temperature sensors are uniformly arranged on the fixed seat; the utility model discloses a cavity, including base, linkage wheel, gear motor, pivot, the one end of pivot is extended to the inside of cavity and is fixedly connected with base plate, the internally mounted of base has vertical pivot, is equipped with the linkage wheel in the pivot, and one side transmission of linkage wheel is connected with gear motor, and the pivot is scalable, and the one end of pivot extends to the inside and the fixedly connected with base plate of cavity, has evenly laid a plurality of vertical.
As a further scheme of the invention: two assembly seats are arranged at the inner part of the cavity at intervals, a horizontal clamping screw rod and a transmission rod are installed between the two assembly seats at intervals, one end of the clamping screw rod extends to the inner part of the assembly seats and is connected with a motor in a transmission mode, the clamping screw rod is a bidirectional screw rod, two installation seats are installed between the clamping screw rod and the transmission rod at intervals, the two installation seats are in reverse transmission fit with the clamping screw rod and are in sliding installation with the transmission rod, and a limiting plate in a circular arc shape is movably installed on each installation seat.
As a further scheme of the invention: a side face, deviating from the culture dish, of the limiting plate is evenly connected with a plurality of sliding rods, the cross sections of the sliding rods are T-shaped, and one ends of the sliding rods extend to the inside of the mounting seat and are connected with clamping springs between the mounting seat and the mounting seat.
As a further scheme of the invention: one side of the assembly seat, which is away from the mounting seat, is fixedly connected with an assembly shaft, the assembly shafts are respectively rotatably mounted with the base frame, two sides of the cavity are fixedly connected with mounting boxes, one of the assembly shafts is provided with a worm wheel, the lower end of the worm wheel is connected with a worm in a transmission manner, the worm is slidably mounted with the fixing seat through the base, and one end of the worm is connected with a motor in a transmission manner; the both sides of bed frame extend to in the mounting box respectively, are connected with horizontally reset spring between one side of bed frame and the mounting box, and the transmission of bed frame opposite side is connected with the drive wheel, and eccentric installation of drive wheel and transmission are connected with the motor.
As a further scheme of the invention: the cross-section of base is T shape, and a plurality of universal wheels have evenly been laid to the lower extreme of base, have laid the locking switch on the universal wheel, have evenly laid a plurality of vertical electric telescopic handle on the base, and electric telescopic handle is located the oblique top of universal wheel, installs the locating piece on electric telescopic handle's the actuating lever.
As a further scheme of the invention: and a protective layer made of rubber is arranged on the contact surface of the limiting plate and the culture dish.
Compared with the prior art, the invention has the beneficial effects that: the device for culturing and separating the novel coronavirus pneumonia vaccine research and development controls the feeding of the two mounting seats through the clamping screw rod, so that a culture dish is conveniently clamped, and the clamping force of the limiting plate on the culture dish is conveniently controlled by arranging the sliding rod and the clamping spring between the limiting plate and the mounting seats, so that the culture dish is prevented from being damaged; the culture dish is convenient to drive to shake as necessary through the matching of the driving wheel and the return spring, the use is convenient, the culture dish is convenient to overturn through the meshing of the worm and gear, the scraping plate can be driven to slide along the inner wall of the culture dish through the feeding bolt, the culture substance is convenient to separate from the culture dish, and the discharge is convenient; through fan and temperature sensor's cooperation, be convenient for control the temperature in the cavity for the culture dish obtains suitable cultivation condition, rotates the inside air current that can stir the cavity through ordering about the stirring leaf, makes culture dish thermally equivalent or cooling, ensures the quality of cultivating.
Drawings
Fig. 1 is a schematic structural diagram of a culturing and separating device for developing a novel coronavirus pneumonia vaccine.
Fig. 2 is a schematic diagram of the movable installation of the limiting plate and the assembling seat in the culturing and separating device for developing the novel coronavirus pneumonia vaccine.
FIG. 3 is a schematic diagram showing the layout of scraper plates in a culture dish in a culture and separation device for research and development of novel coronavirus pneumonia vaccines.
Fig. 4 is a schematic diagram showing the movable connection between the limiting plate and the mounting seat in the culturing and separating device for developing the novel coronavirus pneumonia vaccine.
In the figure: 1-a base, 2-universal wheels, 3-electric telescopic rods, 4-positioning blocks, 5-a cavity, 6-a sealing cover, 7-a fan, 8-a mounting box, 9-a rotating shaft, 10-a linkage wheel, 11-a base plate, 12-a stirring blade, 13-a fixed seat, 14-a temperature sensor, 15-a driving wheel and 16-a base frame, 17-a return spring, 18-an assembly seat, 19-an assembly shaft, 20-a clamping screw rod, 21-an installation seat, 22-a culture dish, 23-a worm wheel, 24-a worm, 25-a transmission rod, 26-a limiting plate, 27-a sliding rod, 28-a sample adding groove, 29-a scraping plate, 30-a guide rod, 31-a feeding bolt and 32-a clamping spring.
Detailed Description
In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only used for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be configured and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Example 1
Referring to fig. 1-4, a novel device for culturing and separating a coronavirus pneumonia vaccine for research and development comprises a base 1, wherein a cavity 5 is fixed on the base 1, the cavity 5 is provided with a sealing cover 6, a culture dish 22 is movably mounted in the cavity 5, the culture dish 22 comprises an upper cover and a lower cover which are matched with each other, an openable sample adding groove 28 is distributed on the upper cover, a scraper 29 is movably mounted on the inner side of the lower cover, a feed bolt 31 is rotatably mounted at the center of the lower end of the scraper 29, one end of the feed bolt 31 extends to the outer side of the culture dish 22, a plurality of guide rods 30 are uniformly mounted between the lower end of the scraper 29 and the culture dish 22, and the guide rods 30 are telescopic so as to drive the scraper 29 to slide through the feed bolt 31 and separate the vaccine from the culture dish 22.
Further, the inner side of the cavity 5 is connected with a circular fixing seat 13, the fixing seat 13 is located below the culture dish 22, the inner side of the cavity 5 is uniformly provided with a plurality of inclined fans 7, the fans 7 are located above the culture dish 22, and the fixing seat 13 is uniformly provided with a plurality of temperature sensors 14 for monitoring the temperature inside the cavity 5, so that the culture dish 22 obtains a proper culture temperature; the internally mounted of base 1 has vertical pivot 9, is equipped with linkage wheel 10 in the pivot 9, and one side transmission of linkage wheel 10 is connected with gear motor, and pivot 9 is scalable, and the one end of pivot 9 extends to the inside and the fixedly connected with base plate 11 of cavity 5, evenly has laid a plurality of vertical stirring leaves 12 on the base plate 11 for stir the inside air current of cavity 5, make culture dish 22 thermally equivalent or cool off.
Further, two assembly seats 18 have been laid at the inside interval of cavity 5, horizontal clamping lead screw 20 and transfer line 25 are installed at the interval between two assembly seats 18, the inside and the transmission that the one end of clamping lead screw 20 extended to assembly seats 18 are connected with the motor, clamping lead screw 20 is two-way lead screw, two mount pads 21 are installed at the interval between clamping lead screw 20 and the transfer line 25, two mount pads 21 and the reverse transmission cooperation of clamping lead screw 20 and with transfer line 25 slidable mounting, movable mounting has convex limiting plate 26 on the mount pad 21, be convenient for carry out the centre gripping to culture dish 22.
Further, a side that limiting plate 26 deviates from culture dish 22 evenly is connected with a plurality of slide bars 27, and the cross-section of slide bar 27 is the T shape, and the one end of slide bar 27 extends to the inside of mount pad 21 and be connected with clamping spring 32 between the mount pad 21 to control limiting plate 26 avoids damaging culture dish 22 to the clamping-force of culture dish 22.
Furthermore, one side of the assembling seat 18, which is far away from the mounting seat 21, is fixedly connected with an assembling shaft 19, the assembling shafts 19 are respectively rotatably mounted with the base frame 16, two sides of the cavity 5 are fixedly connected with the mounting boxes 8, one of the assembling shafts 19 is provided with a worm wheel 23, the lower end of the worm wheel 23 is in transmission connection with a worm 24, the worm 24 is slidably mounted with the fixing seat 13 via the base, and one end of the worm 24 is in transmission connection with a motor; the both sides of bed frame 16 extend to in the mounting box 8 respectively, are connected with horizontally reset spring 17 between one side of bed frame 16 and the mounting box 8, and the transmission of bed frame 16 another side is connected with drive wheel 15, and drive wheel 15 eccentric mounting and transmission are connected with the motor to in order to cooperate with reset spring 17 and order about the culture dish 22 after the clamping and carry out necessary rocking, make things convenient for the cultivation of bacterin.
Further, the cross-section of base 1 is the T shape, and a plurality of universal wheels 2 have evenly been laid to the lower extreme of base 1, have laid the locking switch on the universal wheel 2, have evenly laid a plurality of vertical electric telescopic handle 3 on the base 1, and electric telescopic handle 3 is located the oblique top of universal wheel 2, installs locating piece 4 on electric telescopic handle 3's the executive rod, so that the position of device removes and fixed placing, convenient to use.
The working principle of the embodiment is as follows:
during actual use, the clamping screw rod 20 is started to operate according to the specification of the culture dish 22, the clamping screw rod 20 drives the two mounting seats 21 to approach each other, so that the two limiting plates 26 clamp the culture dish 22, and along with the operation of the clamping screw rod 20, the limiting plates 26 extrude the clamping springs 32 through the sliding rods 27, so that the clamping force of the limiting plates 26 on the culture dish 22 is controlled conveniently; the sample adding slot 28 is opened to facilitate adding necessary culture raw materials into the culture dish 22, when necessary shaking is needed, the driving wheel 15 is started to slowly run, the driving wheel 15 is matched with the return spring 17 to facilitate driving the culture dish 22 to shake, and the culture is facilitated; setting the air outlet temperature of the fan 7 according to the requirement of cultivation, starting the rotating shaft 9 to operate, wherein the rotating shaft 9 stretches and retracts to adjust the extending position of the stirring blade 12, the rotating shaft 9 drives the stirring blade 12 to operate, so that the air flow in the cavity 5 is stirred conveniently, the culture dish 22 is heated or cooled uniformly, the temperature sensor 14 monitors the temperature in the cavity 5 in real time, and the start and stop of the fan 7 are controlled conveniently; after the culture is completed, the starting worm 24 is meshed with the worm wheel 23, so that the culture dish 22 is conveniently overturned, the cultured substances are conveniently taken out, when the culture dish 22 is adhered, the scraper plate 29 is driven to move along the culture dish 22 by rotating the feeding bolt 31, the culture substances are conveniently and completely separated from the culture dish 22, and the use is convenient.
Example 2
In order to avoid damaging the culture dish 22 during clamping, the present embodiment is modified from embodiment 1 by the following: the contact surface of the limiting plate 26 and the culture dish 22 is provided with a protective layer made of rubber, so that the outer surface of the culture dish 22 is protected, and the clamping operation is convenient.
It should be noted that, although the present specification describes embodiments, each embodiment does not include only a single technical solution, and such description of the specification is only for clarity, and those skilled in the art should take the specification as a whole, and the technical solutions in the embodiments may be appropriately combined to form other embodiments understood by those skilled in the art, and the above-mentioned embodiments only express the preferred embodiments of the technical solutions, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the claims of the technical solutions. It should be noted that, for those skilled in the art, various modifications, improvements and substitutions can be made without departing from the spirit of the invention, and all of them belong to the protection scope of the technical solution. The protection scope of this technical solution patent should be subject to the appended claims.

Claims (3)

1. The utility model provides a novel coronavirus pneumonia vaccine research and development is with device of cultivateing and separation, includes base (1), its characterized in that, be fixed with cavity (5) on base (1), cavity (5) are equipped with sealed lid (6), and the inside movable mounting of cavity (5) has culture dish (22), culture dish (22) are including the upper cover and the lower cover of mutually supporting, and upper cover has laid application of sample groove (28) that can open, and the inboard movable mounting of lower cover has scraping plate (29), and the lower extreme center department of scraping plate (29) rotates and installs feed bolt (31), and the one end of feed bolt (31) extends to the outside of culture dish (22), evenly installs a plurality of guide bars (30) between scraping plate (29)'s lower extreme and culture dish (22), and guide bar (30) are scalable, the inboard of cavity (5) is connected with annular fixing base (13), the fixed seat (13) is positioned below the culture dish (22), the inner side of the cavity (5) is uniformly provided with a plurality of inclined fans (7), the fans (7) are positioned above the culture dish (22), and the fixed seat (13) is uniformly provided with a plurality of temperature sensors (14); the novel rotary clamping machine is characterized in that a vertical rotating shaft (9) is arranged inside the base (1), a linkage wheel (10) is assembled on the rotating shaft (9), a speed reducing motor is connected to one side of the linkage wheel (10) in a transmission manner, the rotating shaft (9) is telescopic, one end of the rotating shaft (9) extends to the inside of the cavity (5) and is fixedly connected with a substrate (11), a plurality of vertical stirring blades (12) are uniformly distributed on the substrate (11), two assembling seats (18) are distributed in the cavity (5) at intervals, a horizontal clamping screw rod (20) and a transmission rod (25) are installed between the two assembling seats (18) at intervals, one end of the clamping screw rod (20) extends to the inside of the assembling seats (18) and is connected with a motor in a transmission manner, the clamping screw rod (20) is a bidirectional screw rod, two installing seats (21) are installed between the clamping screw rod (20) and the transmission rod (25) at intervals, and the two installing seats (21) are in reverse, a circular arc-shaped limiting plate (26) is movably mounted on the mounting seat (21), a plurality of sliding rods (27) are uniformly connected to one side surface of the limiting plate (26) departing from the culture dish (22), the cross sections of the sliding rods (27) are T-shaped, one ends of the sliding rods (27) extend into the mounting seat (21), clamping springs (32) are connected between the sliding rods and the mounting seat (21), one side of the assembling seat (18) departing from the mounting seat (21) is fixedly connected with an assembling shaft (19), the assembling shaft (19) is respectively rotatably mounted with the base frame (16), the two sides of the cavity (5) are fixedly connected with mounting boxes (8), a worm wheel (23) is mounted on one of the assembly shafts (19), the lower end of the worm wheel (23) is connected with a worm (24) in a transmission manner, the worm (24) is slidably mounted with the fixed seat (13) through a base, and one end of the worm (24) is connected with a motor in a transmission manner; the both sides of bed frame (16) extend to in mounting box (8) respectively, are connected with horizontally reset spring (17) between one side of bed frame (16) and mounting box (8), and the transmission of bed frame (16) another side is connected with drive wheel (15), and drive wheel (15) eccentric mounting and transmission are connected with the motor.
2. The novel culture and separation device for research and development of coronavirus pneumonia vaccines as claimed in claim 1, wherein the cross section of the base (1) is T-shaped, a plurality of universal wheels (2) are uniformly distributed at the lower end of the base (1), locking switches are distributed on the universal wheels (2), a plurality of vertical electric telescopic rods (3) are uniformly distributed on the base (1), the electric telescopic rods (3) are located obliquely above the universal wheels (2), and positioning blocks (4) are mounted on actuating rods of the electric telescopic rods (3).
3. The device for culturing and separating the research and development of the novel coronavirus pneumonia vaccine as claimed in claim 1, wherein a protective layer made of rubber is arranged on the contact surface of the limiting plate (26) and the culture dish (22).
CN202011388069.8A 2020-12-01 2020-12-01 Novel research and development of coronavirus pneumonia vaccine is with device of cultivateing and separation Active CN112481126B (en)

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