CN112472658A - Skin care gel bead and preparation method thereof - Google Patents

Abstract

The invention discloses a skin care gel bead and a preparation method thereof, the skin care gel bead comprises the following components by weight: 0.1-5 parts of wrapping agent, 10-90 parts of stem cell active ingredient solution and 20-100 parts of water. The preparation method comprises the following steps: weighing the wrapping agent, the stem cell active ingredient solution and water according to the weight ratio; dispersing the wrapping agent in water, adding the stem cell active ingredient solution, and stirring and mixing uniformly; and dropwise adding the obtained mixed solution into pre-cooled receiving liquid, and filtering after solidification to obtain the coagulated beads. According to the invention, the stem cell active ingredients are wrapped by the gel, so that the effects of the active ingredients are protected; the stem cell active ingredient solution is solidified into balls, and the embedding rate is almost 100%; the bead has simple components, low-temperature operation and easy preparation method, does not influence the activity of the active ingredients, and does not influence if the active ingredients contain salt; the gel beads are convenient to use and can release active ingredients only by smearing.

Description

Skin care gel bead and preparation method thereof

Technical Field

The invention relates to the technical field of biological medicines, in particular to a skin care gel bead and a preparation method thereof.

Background

The stem cells can secrete hundreds of cytokines, polypeptides and the like, such as epidermal growth factors, transforming growth factor beta, platelet-derived growth factors, fibroblast growth factors, insulin-like growth factors, vascular endothelial growth factors and the like, and current researches also prove that the secretions of the stem cells have certain skin care effects. However, the active ingredients secreted by stem cells are very delicate and expensive, and some ingredients commonly used in skin care products, such as oil, alcohol, emulsifier, preservative, etc., affect the stability of the active ingredients, limiting the range of application thereof.

The micro-bead technology is a hot spot in the field of cosmetics. The microcapsule can wrap active ingredients, improve the stability of the active ingredients, achieve the aim of slow release, and can isolate mutually influenced ingredients through a microcapsule technology to ensure that the ingredients coexist peacefully in a product.

The stem cell active ingredients are directly added into the products in the current stem cell products, and the risk of influencing the activity is influenced. In this regard, a technique of loading a stem cell active ingredient on microspheres has been developed. Patent CN 102885710B discloses a sodium alginate/chitosan/collagen composite microsphere loaded with cell growth factors, which needs to be swelled with solvent when in use, thus increasing the using steps of consumers. In addition, the method mainly depends on gel formed by combining sodium alginate and divalent cations, and inorganic salt contained in the supernatant of the stem cells influences the gelation of the sodium alginate. Patent CN 109303761 a discloses a chitosan microsphere composition loaded with stem cell active factors, which can be directly applied, but the embedding rate is only about 60% at the highest. Therefore, it is required to develop a method for preparing microcapsules containing stem cell active ingredients, which is simple to operate, high in encapsulation efficiency and convenient to use.

Disclosure of Invention

The invention mainly solves the technical problem of providing a skin care gel bead and a preparation method thereof, the gel bead has simple components, the preparation method is easy, the embedding rate is almost 100 percent, the active components are prevented from being damaged as much as possible, the use of the gel bead is convenient, and the active components can be released only by smearing.

In order to solve the technical problems, the invention adopts a technical scheme that: provides a skin care coagulated bead which comprises a wrapping agent, a stem cell active ingredient solution and water, wherein the weight ratio of each component is as follows: 0.1-5 parts of wrapping agent, 10-90 parts of stem cell active ingredient solution and 20-100 parts of water.

In a preferred embodiment of the present invention, the coating agent is one or more of agar, sodium alginate, chitosan, hydroxyethyl cellulose, gelatin, carrageenan, xanthan gum, sclerotium rolfsii gum, gum arabic and gellan gum.

In a preferred embodiment of the invention, the stem cell active ingredient solution is one or more of an umbilical cord extract, a placenta extract, a stem cell culture supernatant and a plant tissue culture.

In a preferred embodiment of the present invention, the stem cell culture supernatant is obtained by culturing and expanding stem cells with a serum-free medium, collecting the culture supernatant during liquid change and passage, and filtering to obtain a stem cell active factor component solution.

In a preferred embodiment of the present invention, the receiving solution is one of a sodium tripolyphosphate solution and a NaOH solution.

In a preferred embodiment of the present invention, the receiving solution is a pre-cooled receiving solution with a temperature of 0-4 ℃.

In order to solve the technical problem, the invention adopts another technical scheme that: provides a preparation method of skin care beads, which comprises the following steps:

(1) weighing the raw materials according to the weight ratio;

(2) dispersing the wrapping agent in water, adding the stem cell active ingredient solution, and stirring and mixing uniformly;

(3) dripping the obtained mixed solution into pre-cooled receiving liquid drop by drop, solidifying for 30-180min, and filtering to obtain coagulated beads.

In a preferred embodiment of the present invention, the volume ratio of the pre-cooled receiving solution and the mixed solution in step (3) is (1-5): 1.

the invention has the beneficial effects that: according to the invention, the stem cell active ingredient is wrapped by the gel, so that the direct contact with other ingredients is avoided, and the efficacy of the active ingredient is protected; the stem cell active ingredient solution is solidified into balls, and the embedding rate is almost 100%; the bead has simple components, low-temperature operation and easy preparation method, does not influence the activity of the active ingredients, and does not influence if the active ingredients contain salt; the gel beads are convenient to use and can release active ingredients only by smearing.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Weighing 0.5 part of chitosan, 0.5 part of carrageenan, 30 parts of dry cell active ingredient solution and 69 parts of water according to the weight ratio; dispersing 0.5 part of chitosan and 0.5 part of carrageenan in 69 parts of water, adding 30 parts of dry cell active ingredient solution, and stirring and mixing uniformly; dropwise adding the obtained mixed solution into a pre-cooled sodium tripolyphosphate solution at 0 ℃, wherein the volume ratio of the pre-cooled receiving solution to the mixed solution is 2: 1, solidifying for 120 min, and filtering to obtain the coagulated beads.

Example 2

Weighing 0.5 part of chitosan, 0.3 part of agar, 0.3 part of xanthan gum, 70 parts of stem cell active ingredient solution and 29 parts of water according to the weight ratio; dispersing 0.5 part of chitosan, 0.3 part of agar and 0.3 part of xanthan gum in 29 parts of water, adding 70 parts of stem cell active ingredient solution, and stirring and mixing uniformly; dropwise adding the obtained mixed solution into a pre-cooled sodium tripolyphosphate solution at the temperature of 2 ℃, wherein the volume ratio of the pre-cooled receiving solution to the mixed solution is 4: 1, solidifying for 150 min, and filtering to obtain the coagulated beads.

Example 3

Preparing the condensed pearl essence: uniformly mixing 16 parts of glycerol, 0.2 part of hydroxyethyl cellulose, 0.1 part of gellan gum, 0.7 part of phenoxyethanol and 83 parts of pure water to obtain the essence matrix phase. The essence matrix phase was mixed with the stem cell active ingredient beading of example 2 at a ratio of 1:1 to obtain a beading essence. When in use, the gel beads can be broken only by smearing, and stem cell active ingredients are released.

Comparative example 1

Preparation of conventional essence: mixing 8 parts of glycerol, 0.3 part of hydroxyethyl cellulose, 0.7 part of phenoxyethanol and 56 parts of water uniformly, adding 35 parts of dry cell active ingredient solution, and mixing uniformly to obtain the cell growth promoting agent.

Experimental example 1

Selecting 40 test persons with no obvious difference in skin quality and water content under the condition of room temperature and relative humidity of 50-60%, and carrying out essence performance experiments when the age is 32-38 years, wherein specifically 20 volunteers use the cream-coagulant essence of the embodiment 3, and the rest 20 patients use the essence of the comparative example 1, and the essence is used before sleep every day and continuously used for 4 weeks. Before, after two weeks and four weeks of sample trial, the area of skin wrinkles around eyes was measured by using a Vissionline VI650 skin wrinkle tester in a room at a temperature of 25 ℃. + -. 1 ℃ and a humidity of 50% +/-5%, and the percentage of improvement in the area ratio of wrinkles before and after using the essence was calculated, and the results are shown in Table 1.

TABLE 1

As can be seen from table 1, the beading-coated stem cell active essence showed better wrinkle improvement ability, indicating that the coated stem cell active ingredient was better maintained in efficacy.

The skin care gel bead and the preparation method thereof have the beneficial effects that: the stem cell active ingredients are wrapped by the gel, so that the direct contact with other ingredients is avoided, and the efficacy of the active ingredients is protected; the stem cell active ingredient solution is solidified into balls, and the embedding rate is almost 100%; the bead has simple components, low-temperature operation and easy preparation method, does not influence the activity of the active ingredients, and does not influence if the active ingredients contain salt; the gel beads are convenient to use and can release active ingredients only by smearing.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (8)

1. The skin care gel bead is characterized by comprising the following components in parts by weight: 0.1-5 parts of wrapping agent, 10-90 parts of stem cell active ingredient solution and 20-100 parts of water.

2. The skin care bead set of claim 1, wherein said coating agent is one or more of agar, sodium alginate, chitosan, hydroxyethyl cellulose, gelatin, carrageenan, xanthan gum, sclerotium rolfsii gum, gum arabic, and gellan gum.

3. The skin care bead set of claim 1, wherein said stem cell active ingredient solution is one or more of an umbilical cord extract, a placental extract, a stem cell culture supernatant, and a plant tissue culture.

4. The skin care beading according to claim 3, wherein the culture supernatant of the stem cells is obtained by culturing and amplifying the stem cells by a serum-free medium, collecting the culture supernatant during liquid change and passage, and filtering the culture supernatant to obtain the active factor component solution of the stem cells.

5. The skin care bead set of claim 1, wherein said receiving fluid is one of a sodium tripolyphosphate solution and a NaOH solution.

6. The skin care bead set of claim 1, wherein said receiving fluid is a pre-chilled receiving fluid at 0-4 ℃.

7. The method of making a skin care bead according to claim 1, comprising the steps of:

(1) weighing the wrapping agent, the stem cell active ingredient solution and water according to the weight ratio;

(2) dispersing the wrapping agent in water, adding the stem cell active ingredient solution, and stirring and mixing uniformly;

(3) dripping the obtained mixed solution into pre-cooled receiving liquid drop by drop, solidifying for 30-180min, and filtering to obtain coagulated beads.

8. The preparation method according to claim 7, wherein the volume ratio of the pre-cooled receiving solution in the step (3) to the mixed solution is (1-5): 1.