CN112461906A - Preparation and application of biosensor based on N-graphene nanoribbon-gold platinum nanocluster - Google Patents

Preparation and application of biosensor based on N-graphene nanoribbon-gold platinum nanocluster Download PDF

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CN112461906A
CN112461906A CN202011285817.XA CN202011285817A CN112461906A CN 112461906 A CN112461906 A CN 112461906A CN 202011285817 A CN202011285817 A CN 202011285817A CN 112461906 A CN112461906 A CN 112461906A
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gold
graphene nanoribbon
platinum nanocluster
graphene
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张娟
赵丽芳
滕龙
于燕萍
孙文丹
邹惠玲
于晓菲
田静
刘鹏飞
齐云
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Shandong Institute for Product Quality Inspection
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Abstract

The invention belongs to the technical field of biosensors, and relates to preparation and application of a biosensor based on N-graphene nanoribbons-gold platinum nanoclusters. And modifying by using a glassy carbon electrode as a working electrode and an N-graphene nanoribbon-gold platinum nanocluster compound. The method adopts the glassy carbon electrode, is low in price, combines the electrochemical technology and the biosensing technology, improves the selectivity and the sensitivity of the sensor, does not need sample pretreatment on a sample, shortens the detection time, reduces the detection cost, and is a rapid and cheap detection method with high sensitivity and selectivity.

Description

Preparation and application of biosensor based on N-graphene nanoribbon-gold platinum nanocluster
Technical Field
The invention belongs to the technical field of biosensors, and relates to preparation and application of a biosensor based on N-graphene nanoribbons-gold platinum nanoclusters.
Background
Ascorbic Acid (AA) is a water-soluble vitamin (vitamin C) which is essential for human life activities and plays a regulatory role in redox metabolism. The human body cannot prepare vitamin C by itself, and if the body is largely deficient in vitamin C, it may cause scurvy.
Dopamine (DA) is synthesized and secreted from the human brain, can affect the human emotion, and is a neurotransmitter in the human brain. This brain endocrine is primarily responsible for the desire of the brain, and the sensation is transmitted as excitement and distraction, and is also associated with addiction. Therefore, the exploration of dopamine concentrations has a critical role in certain conditions (depression, parkinson's disease, alzheimer's disease, etc.).
Uric Acid (UA) is a metabolite of purines. If too much uric acid is produced in the body and excretion is delayed or the excretion mechanism of uric acid is degraded, the uric acid in the body is accumulated too much, so that the body fluid of the human body is changed into acid, and the normal function of cells of the human body is influenced. The hyperuricemia can cause gout, Lesch-Nyhan syndrome, obesity, diabetes, high cholesterol, hypertension and other diseases; and the low level of uric acid can cause nephropathy, Wilson's disease and other diseases.
The content of ascorbic acid, dopamine and uric acid is closely related to human health, so that the method has important application value in detection of AA, DA and UA. AA. DA and UA both have electrochemical activity, but because electrochemical oxidation peak potentials of ascorbic acid, dopamine and uric acid are similar, the selectivity and detection limit of nano-particle, self-assembled film and polymeric film modified electrodes adopted by most detection mechanisms at present cannot meet the modern detection requirements. Therefore, it is necessary to establish a simple and rapid method for simultaneously measuring dopamine, ascorbic acid and uric acid with high sensitivity and good selectivity. Separating and enhancing their electrochemical signals, achieving their simultaneous determination is of great importance not only in the field of biomedical chemistry but also in the field of neurochemistry, as well as providing an effective method for diagnostic and pathological studies.
Disclosure of Invention
The invention provides a preparation method of a biosensor for simultaneously detecting ascorbic acid, dopamine and uric acid, which has the advantages of high sensitivity, good selectivity, good reproducibility, simple operation and low cost, aiming at the defects of complicated instruments and equipment, complicated operation process, high requirement on detection personnel, high detection cost and the like of the traditional detection method.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme:
a biosensor based on N-graphene nanoribbons-gold platinum nanoclusters is characterized in that a glassy carbon electrode is used as a working electrode, and N-graphene nanoribbons-gold platinum nanocluster compounds are used for modification.
The preparation method of the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster comprises the following steps
(1) Mixing N-multi-walled carbon nanotubes with an acidic solution for reaction, and adding KMnO after the reaction4Washing and drying after continuous reaction to obtain a black solid N-graphene nanoribbon;
(2) PVA solution and AuCl-4Mixing the solutions, dropwise adding trisodium citrate solution after vigorous stirring, stirring to generate Au colloid, and adding K2PtCl6Adding a sodium borohydride solution after pure argon saturation of the mixed solution of PVP and PVP, and stirring to obtain a gold-platinum nano cluster;
(3) dissolving the N-graphene nanoribbon in pure water, performing ultrasonic treatment, adding a gold-platinum nanocluster solution, oscillating, centrifuging, removing a supernatant, and dispersing a precipitate into a phosphate buffer solution again to obtain a stock solution of the N-graphene nanoribbon-gold-platinum nanocluster compound;
(4) with A12O3Polishing a glassy carbon electrode with the diameter of 4 mm by using powder, and then washing the glassy carbon electrode with ultrapure water;
(5) and (3) dropwise adding the stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
Preferably, the acidic solution in step (1) is H2SO4And H3PO4The volume ratio is 9: 1, reacting the N-multi-walled carbon nano-tube with the acid solution in a microwave reaction bottle, controlling the temperature of a microwave reactor at 110-140 ℃, and reacting for 2-5 min. Cooling for 10-20min, adding KMnO into the reaction solution4The microwave temperature is controlled to be 65-85 ℃ for reaction for 4-10 min, 0.1-0.5 part by weight of N-multi-walled carbon nano-tube and KMnO42-10 parts.
Preferably, the PVA solution for preparing Au colloid in the step (2) has a concentration of 10mg/mL and AuCl- 4The concentration of the solution is 0.25mM, the PVA solution and AuCl-4The solution is stirred vigorously for 1-2h, then 0.1M fresh trisodium citrate solution is added dropwise, the stirring time is 2h, and the volume parts of the substances are as follows: PVA solution 0.5-1 weight portions and AuCl-4200 parts of solution 170 and 5-12 parts of trisodium citrate.
Preferably, the gold colloid solution is added with 1mM K2PtCl6And 10-50 parts by volume of a mixed solution of 5 mg/mL PVP; and (3) after the mixture is saturated by pure argon for 30min, adding 2.5-5.0 parts by volume of sodium borohydride solution with the mass fraction of 1%, and stirring for 0.5-2h to obtain the gold-platinum nanocluster.
Preferably, 1-2mg of the obtained N-graphene nanoribbon is taken in the step (3), dissolved in 5mL of ultrapure water, ultrasonically treated for 30min, then 2-5mL of gold-platinum nanocluster solution is added, oscillated for 2-3h, centrifuged, the supernatant is removed, and the precipitate is re-dispersed in 2-4mL of phosphate buffer solution, so that the stock solution of the N-graphene nanoribbon-gold-platinum nanocluster compound is obtained.
Preferably, the Au colloid has a particle size of 3.0 to 10 nm.
Preferably, the black solid N-graphene nanoribbon is subjected to vacuum drying treatment at the temperature of 35-40 ℃.
The detection steps are as follows:
connecting a reference electrode, a counter electrode and a working electrode on an electrochemical workstation, adding 10mL of phosphate buffer solution into an electrolytic cell, and adding 5-10 muL of AA, DA and UA with different concentrations into the solution;
setting the potential range to be-0.2-0.7V, and detecting the current response of the working electrode to AA, DA and UA with different concentrations by a differential pulse method;
drawing a working curve according to the relation between the obtained current response and the concentration of AA, DA and UA;
(2) detection of AA, DA and UA
Replacing AA, DA and UA in the step (1) with 5-10 muL of to-be-detected serum sample, and performing other steps as in the step (1);
and according to the measured values, comparing the working curves of AA, DA and UA, and calculating the contents of AA, DA and UA in the sample.
Compared with the prior art, the invention has the advantages and positive effects that:
1. the method adopts the glassy carbon electrode, is low in price, combines the electrochemical technology and the biosensing technology, improves the selectivity and the sensitivity of the sensor, does not need sample pretreatment on a sample, shortens the detection time, reduces the detection cost, and is a rapid and cheap detection method with high sensitivity and selectivity.
2. The method is quick, simple and accurate, and the sample does not need pretreatment, so that the method can be used for simultaneously detecting three substances, namely AA, DA and UA in a complex serum sample.
3. According to the invention, the N-graphene nanoribbon is used as a substrate material, so that the gold-platinum nanoclusters are loaded in a unique core-shell structure, and the loading capacity of the gold-platinum nanoclusters is increased.
The N-graphene nano has large specific surface area and good electrochemical performance, and the gold-platinum nano-cluster has super-strong catalytic performance, so that the increase of electrochemical signals and sensitive conversion and transmission are realized, the sensitivity of the sensor is improved, and the detection limits of AA, DA and UA can be as low as 0.040 mu M, 0.050 mu M and 0.029 mu M.
5. The invention adopts the glassy carbon electrode, has simple preparation, low cost, low reagent consumption, easy miniaturization and integration and wide application range.
Drawings
FIG. 1 is a transmission electron microscope image of N-multi-walled carbon nanotubes.
FIG. 2 is a transmission electron micrograph of N-graphene nanoribbons.
FIG. 3 is an XRD characterization diagram of N-graphene nanoribbons.
Fig. 4 is a working curve for simultaneous detection of three substances.
Detailed Description
In order that the above objects, features and advantages of the present invention may be more clearly understood, the present invention will be further described with reference to specific embodiments. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and thus the present invention is not limited to the specific embodiments of the present disclosure.
Example 1
Preparation of N-graphene nanoribbon-gold platinum nanocluster compound
(1) 0.1g N-multiwall carbon nanotube was mixed with 30mL of H2SO4/H3PO4(9: 1) placing the mixed solution into a microwave reaction bottle, controlling the temperature of a microwave reactor at 110 ℃, and reacting for 2 min. After cooling for 10min, 2g of KMnO was added to the reaction mixture4The microwave temperature is controlled at 65 ℃ for reaction for 4 min. Cooled to room temperature, and thoroughly washed with ultrapure water to neutrality. And drying the obtained black solid, namely the N-graphene nanoribbon in vacuum at the temperature of 35-40 ℃.
(2) 1mL of 10mg/mL PVA solution was mixed with 200mL of 0.25mM AuCl- 4The solution was mixed vigorously and stirred for 1h, then 5mL of 0.1M fresh trisodium citrate solution was added dropwise and the solution stirred for 2h to form Au colloid (particle size 3.0 nm). In advance of the manufactureThe prepared gold colloid solution was added with 10mL of 1mM K2PtCl6And 5 g/L PVP. After 30min of saturation with pure argon, 2.5 mL of 1% sodium borohydride solution is added, and the mixture is stirred for 0.5 to 2h, so that the gold-platinum nanocluster is obtained.
(3) Dissolving 1mg of the obtained N-graphene nanoribbon in 5mL of ultrapure water, performing ultrasonic treatment for 30min, adding 2mL of a gold-platinum nanocluster solution, and oscillating for 2h to load the gold-platinum nanoclusters on the surface of the N-graphene nanoribbon.
And centrifuging the mixture, removing the supernatant, and re-dispersing the precipitate into 2mL of phosphate buffer solution to obtain the stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound.
Preparation of N-graphene nanoribbon-gold platinum nanocluster biosensor
(1) With A12O3The powder was polished to a glassy carbon electrode of 4 mm diameter and then rinsed clean with ultra-pure water.
(2) And (3) dropwise adding the 6 mu L N-graphene nanoribbon-gold platinum nanocluster compound stock solution onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
Example 2
This example differs from example 1 in the preparation of the N-graphene nanoribbon-gold platinum nanocluster biosensor:
(1) with A12O3The powder was polished to a glassy carbon electrode of 4 mm diameter and then rinsed clean with ultra-pure water.
(2) And (3) dropwise adding the 10 mu L N-graphene nanoribbon-gold platinum nanocluster compound stock solution onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
Example 3
Preparation of N-graphene nanoribbon-gold platinum nanocluster compound
(1) 0.5g N-multiwall carbon nanotube and 200mL H2SO4/H3PO4(9: 1) placing the mixed solution into a microwave reaction bottle, controlling the temperature of a microwave reactor at 140 ℃ and reacting for 3 min. Cooling for 15min, adding into the reaction solution10g KMnO4The microwave temperature is controlled at 85 ℃ for reaction for 10 min. Cooled to room temperature, and thoroughly washed with ultrapure water to neutrality. And drying the obtained black solid, namely the N-graphene nanoribbon at 40 ℃ for 1h in vacuum.
(2) 0.5mL of 10mg/mL PVA solution was mixed with 170mL of 0.25mM AuCl- 4The solution was mixed vigorously and stirred for 1h, then 12mL of 0.1M fresh trisodium citrate solution was added dropwise and the solution stirred for 2h to form Au colloid (average particle size 6.0 nm). To the pre-prepared gold colloid solution was added 50mL of a solution containing 1mM K2PtCl6And 5 g/L PVP. And (3) after the mixture is saturated by pure argon for 30min, adding 5mL of sodium borohydride solution with the mass fraction of 1%, and stirring for 2h to obtain the gold-platinum nanocluster.
(3) Dissolving 2mg of the obtained N-graphene nanoribbon in 5mL of ultrapure water, performing ultrasonic treatment for 30min, adding 5mL of a gold-platinum nanocluster solution, and oscillating for 3h to load the gold-platinum nanoclusters on the surface of the N-graphene nanoribbon.
And centrifuging the mixture, removing the supernatant, and re-dispersing the precipitate into 4mL of phosphate buffer solution to obtain the stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound.
Preparing the biosensor of the N-graphene nanoribbon-gold platinum nanocluster:
(1) with A12O3The powder was polished to a glassy carbon electrode of 4 mm diameter and then rinsed clean with ultra-pure water.
(2) And (3) dropwise adding the 6 mu L N-graphene nanoribbon-gold platinum nanocluster compound stock solution onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
Example 4
Preparation of N-graphene nanoribbon-gold platinum nanocluster compound
(1) 0.3 g N-multiwall carbon nanotube was mixed with 120 mL of H2SO4/H3PO4(9: 1) placing the mixed solution into a microwave reaction bottle, controlling the temperature of a microwave reactor at 130 ℃, and reacting for 4 min. After cooling for 15min, 6 g of KMnO was added to the reaction mixture4The microwave temperature is controlled at 70 ℃ for reaction for 8 min. Cooling to room temperature, and treating with ultrasonic waveThe pure water was thoroughly washed to neutrality. And drying the obtained black solid, namely the N-graphene nanoribbon at 35 ℃ in vacuum for 1.5 h.
(2) 0.8mL of 10mg/mL PVA solution was mixed with 180 mL of 0.25mM AuCl- 4The solution was mixed vigorously and stirred for 1h, then 9mL of 0.1M fresh trisodium citrate solution was added dropwise and the solution stirred for 2h to form Au colloid (average particle size 4 nm). 20mL of a gold colloid solution containing 1mM K was added to the gold colloid solution prepared in advance2PtCl6And 5 g/L PVP. After 30min of saturation with pure argon, 3.5mL of 1% sodium borohydride solution is added, and stirring is carried out for 1h, so as to obtain the gold-platinum nanocluster.
(3) Dissolving 1.5mg of the obtained N-graphene nanoribbon in 5mL of ultrapure water, performing ultrasonic treatment for 30min, adding 3mL of a gold-platinum nanocluster solution, and oscillating for 2.5h to load the gold-platinum nanoclusters on the surface of the N-graphene nanoribbon.
And centrifuging the mixture, removing the supernatant, and re-dispersing the precipitate into 3mL of phosphate buffer solution to obtain the stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound.
Preparing the biosensor of the N-graphene nanoribbon-gold platinum nanocluster:
(1) with A12O3The powder was polished to a glassy carbon electrode of 4 mm diameter and then rinsed clean with ultra-pure water.
(2) And (3) dropwise adding the 6 mu L N-graphene nanoribbon-gold platinum nanocluster compound stock solution onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
Detection of AA, DA and UA
The biosensor based on the N-graphene nanoribbon-gold platinum nanocluster prepared in example 1 is used for detection of AA, DA, and UA, and includes the following steps;
(1) drawing of working curves
Preparing a series of N-graphene nanoribbons-gold platinum nanocluster biosensors according to the method described in any one of examples 1-2 to measure AA, DA, and UA at different concentrations;
connecting a reference electrode, a counter electrode and a working electrode (a platinum wire electrode is used as the counter electrode, a saturated calomel electrode is used as the reference electrode, a glassy carbon electrode, namely a modified electrode is used as the working electrode) on an electrochemical workstation, adding 10mL of phosphate buffer solution into an electrolytic cell, and adding 10 muL of AA, DA and UA with different concentrations into the solution;
setting the potential range to be-0.2-0.7V, and detecting the current response of the working electrode to AA, DA and UA with different concentrations by a differential pulse method;
drawing a working curve according to the relation between the obtained current response and the concentration of AA, DA and UA; as shown in FIG. 4, the detection limits of AA, DA, UA can be as low as 0.040. mu.M, 0.050. mu.M, and 0.029. mu.M.
(2) Detection of AA, DA and UA
Replacing AA, DA and UA in the step (1) with 10 mu L of to-be-detected serum sample, wherein other steps are the same as those in the step (1);
and according to the measured values, comparing the working curves of AA, DA and UA, and calculating the contents of AA, DA and UA in the sample.
2. Material characterization
Transmission electron micrographs of the N-multiwalled carbon nanotube and the N-graphene nanoribbon are respectively shown in fig. 1 and fig. 2, and after potassium permanganate stripping microwave treatment, lamellar graphene is stripped from the periphery of the carbon nanotube wall, thereby obtaining the N-graphene nanoribbon. To further demonstrate that N-graphene nanoribbons were synthesized, X-ray diffraction analysis (XRD) characterization was performed on the resulting materials. As shown in FIG. 3, it can be seen that a distinct graphite characteristic peak appears at 2 θ ≈ 25 °, indicating that the N-doped graphene nanoribbon is successfully prepared.
3. Sensor sensitivity and stability experiments
The method for controlling a single variable is adopted, the sensor is used for simultaneously detecting the three substances, and then the change of the concentration of the single substance is controlled, so that the sensor is proved to have higher sensitivity and good stability to the three substances.
TABLE 1 test of sensor sensitivity and stability
Figure 967201DEST_PATH_IMAGE001
4. Detection of actual sample by sensor
And constructing a sensor according to the implementation steps for detecting the vitamin C injection, the dopamine hydrochloride injection and the urine of the actual sample.
And (3) taking a phosphate buffer solution with the pH value of 7.0 as a diluent to respectively dilute the actual samples and then measuring the content of the actual samples. And then adding a certain amount of standard solution into the test solution, measuring the concentration after adding the standard, and calculating the relative standard deviation and the standard adding recovery rate. The results are shown in Table 2.
TABLE 2 actual sample test results
Figure 215780DEST_PATH_IMAGE002
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention in other forms, and any person skilled in the art may apply the above modifications or changes to the equivalent embodiments with equivalent changes, without departing from the technical spirit of the present invention, and any simple modification, equivalent change and change made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical spirit of the present invention.

Claims (10)

1.A biosensor based on N-graphene nanoribbons-gold platinum nanoclusters is characterized in that a glassy carbon electrode is used as a working electrode, and N-graphene nanoribbons-gold platinum nanocluster compounds are used for modification.
2. The method for preparing the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster of claim 1, comprising the steps of
(1) Mixing N-multi-walled carbon nanotubes with an acidic solution for reaction, and adding KMnO after the reaction4Washing and drying after continuous reaction to obtain a black solid N-graphene nanoribbon;
(2) PVA solution and AuCl-4Solutions ofMixing, stirring vigorously, adding trisodium citrate solution dropwise, stirring to obtain Au colloid, adding K2PtCl6Adding a sodium borohydride solution after pure argon saturation of the mixed solution of PVP and PVP, and stirring to obtain a gold-platinum nano cluster;
(3) dissolving the N-graphene nanoribbon in pure water, performing ultrasonic treatment, adding a gold-platinum nanocluster solution, oscillating, centrifuging, removing a supernatant, and dispersing a precipitate into a phosphate buffer solution again to obtain a stock solution of the N-graphene nanoribbon-gold-platinum nanocluster compound;
(4) with A12O3Polishing a glassy carbon electrode with the diameter of 4 mm by using powder, and then washing the glassy carbon electrode with ultrapure water;
(5) and (3) dropwise adding the stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound onto a glassy carbon electrode, and airing to obtain the N-graphene nanoribbon-gold platinum nanocluster-based biosensor.
3. The method for preparing the biosensor based on the N-graphene nanoribbon-gold platinum nanoclusters of claim 2, wherein the acidic solution in the step (1) is H2SO4And H3PO4The volume ratio is 9: 1, reacting the N-multi-walled carbon nano-tube with the acid solution in a microwave reaction bottle, controlling the temperature of a microwave reactor at 110-140 ℃, and reacting for 2-5 min.
4. Cooling for 10-20min, adding KMnO into the reaction solution4The microwave temperature is controlled to be 65-85 ℃ for reaction for 4-10 min, 0.1-0.5 part by weight of N-multi-walled carbon nano-tube and KMnO42-10 parts.
5. The method for preparing the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster of claim 2, wherein the PVA solution for preparing the Au colloid in the step (2) has a concentration of 10mg/mL and AuCl- 4The concentration of the solution is 0.25mM, the PVA solution and AuCl-4The solution is stirred vigorously for 1-2h, then 0.1M fresh trisodium citrate solution is added dropwise, the stirring time is 2h, and the volume parts of the substances are as follows: PVA solution0.5-1 part of liquid and AuCl-4200 parts of solution 170 and 5-12 parts of trisodium citrate.
6. The method for preparing the biosensor based on N-graphene nanoribbons-gold platinum nanoclusters of claim 4, wherein 1mM K is added to the gold colloid solution2PtCl6And 10-50 parts by volume of a mixed solution of 5 mg/mL PVP; and (3) after the mixture is saturated by pure argon for 30min, adding 2.5-5.0 parts by volume of sodium borohydride solution with the mass fraction of 1%, and stirring for 0.5-2h to obtain the gold-platinum nanocluster.
7. The preparation method of the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster of claim 2 is characterized in that 1-2mg of the obtained N-graphene nanoribbon is taken in the step (3), dissolved in 5mL of ultrapure water, subjected to ultrasonic treatment for 30min, added with 2-5mL of gold platinum nanocluster solution, oscillated for 2-3h, centrifuged, the supernatant is removed, and the precipitate is redispersed in 2-4mL of phosphate buffer solution, so that a stock solution of the N-graphene nanoribbon-gold platinum nanocluster compound is obtained.
8. The method for preparing the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster of claim 4, wherein the particle size of Au colloid is 3.0-10 nm.
9. The preparation method of the biosensor based on the N-graphene nanoribbon-gold platinum nanocluster according to claim 4, wherein the black solid N-graphene nanoribbon is subjected to vacuum drying treatment at 35-40 ℃.
10. The method for simultaneously detecting AA, DA and UA by the N-graphene nanoribbon-gold platinum nanocluster-based biosensor according to any one of claims 1 to 8, wherein a reference electrode, a counter electrode and a working electrode are connected to an electrochemical workstation, 10mL of phosphate buffer solution is added into an electrolytic cell, and 5 to 10 μ L of AA, DA and UA solutions with different concentrations are added into the solution; setting the potential range to be-0.2-0.7V, and detecting the current response of the working electrode to AA, DA and UA solutions with different concentrations by a differential pulse method; drawing a working curve according to the relation between the obtained current response and the concentration of AA, DA and UA;
and replacing AA, DA and UA solutions with 5-10 mu L of to-be-detected serum samples, and calculating the AA, DA and UA contents in the samples according to the measured values and the working curve.
CN202011285817.XA 2020-11-17 2020-11-17 Preparation and application of biosensor based on N-graphene nanoribbon-gold platinum nanocluster Pending CN112461906A (en)

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