CN107576702A - A kind of electrochemical sensor preparation method for being used for the Concentration Testings of galectin 3 in serum - Google Patents
A kind of electrochemical sensor preparation method for being used for the Concentration Testings of galectin 3 in serum Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims description 8
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- Peptides Or Proteins (AREA)
Abstract
The present invention relates to the preparation method and application of the electrochemical sensor of the blood concentration level's detection for the insulin sensitivity reduction early diagnosis biomarker galectin 3 (Gal 3) for triggering diabetes B, belong to technical field of electrochemical detection.It is characterized in that：N GNRs Fe MOFs@AuPNs sensor bases modification nano composite material and the nano combined signal materials of AuPt MB are respectively synthesized first, then detection antibody can be specifically bound with Gal 3 and is mixed with AuPt MB composites, and bio signal probe is made.Then the specificity capture antibody of Gal 3 is engaged in the substrate platform of N GNRs Fe MOFs@AuPNs composites modification, in order to specificity capture object Gal 3.The specific binding for detecting antibody with it finally by Gal 3 and capturing antibody, has been prepared the electrochemical sensor of Gal 3 blood concentration level's detection, and the sensor is successfully used in the detection of Gal 3 in serum.The advantage of the invention is that high sensitivity, high specificity, detection is rapid, convenient.The present invention provides the diagnosis basis of early stage for the insulin resistance of initiation diabetes B.
Description
Technical field：
Early diagnosis biomarker-galectin- is reduced the present invention relates to the insulin sensitivity of diabetes B is triggered
The preparation method and application of the electrochemical sensor of 3 (Gal-3) blood concentration level's detection, is based especially on AuPt-MB and receives
The interlayer type immunity biosensor that nano composite material is prepared as novel signal probe, for galectin-3 in serum
(Gal-3) Concentration Testing, field of electrochemical detection is belonged to.
Background technology：
Diabetes are increased rapidly in the world, and human health has been constituted a serious threat.Particularly by insulin spirit
Diabetes B (T2D) caused by sensitivity reduction can result in the cardiovascular complication of lethal, and occupy diabetes prevalence
90%.Found in nearest research, galectin-3 (Gal-3) can be combined directly with insulin receptor suppresses downstream insulin
Acceptor (IR) signal transduction and link inflammation reduce insulin sensitivity, ultimately result in insulin resistance and glucose intolerance,
Trigger diabetes B.Therefore, the change of Gal-3 concentration levels in blood is monitored, it is outstanding to prevention T2D and its cardiovascular complication
To be important.
Have in the traditional mainstay detected at present to Gal-3：Enzyme linked immunosorbent assay (ELISA) (ELISA), immune group
Change and " conventional Gal-3 " determines (Abbott Laboratories' diagnosis).However, these conventional methods are relative there is costliness, time-consuming, sxemiquantitative, need
Skilled operator is wanted, the limitation for the shortcomings of sensitivity finite sum range of linearity is narrow.Facing for early detection can not particularly be met
Bed demand.Therefore, a kind of simplicity for Gal-3 of exploratory development, quick and sensitive detection method seem increasingly important.With biography
The detection method of system is compared, and electro-chemistry immunity passes biological sensor detection method because it has a high sensitivity, high specificity and quick
The advantages that detection, increasingly it is taken seriously in clinical protein biomarker context of detection in recent years.Meanwhile based on nano material
Electrochemica biological sensor due to simplicity, quickly, cost is low, the advantage such as sensitivity height and be widely used in biological sample
Detection.Therefore, a kind of sandwich electro-chemistry immunity biology sensor is built based on nano composite material to realize Gal-3 in serum
Low concentration it is highly sensitive detection provide a kind of new approaches.
Methylene blue (MB) is a kind of derivative of phenothiazine dyes, due to sulphur and nitrogen rich in electronics in its structure be present
Hetero atom is applied to catalysis, sensing and redox as a kind of emerging electrochemical redox active material by extensive land used
The fields such as instruction.However, because its uneven form makes its application in terms of immunosensor signal probe receive limitation.
To solve this problem, the present invention is innovatively introduced with specific surface area is big, electric conductivity is strong and biomolecule is compatible good
With MB oxidative polymerization occurs for AuPt bimetal nanos material, has obtained having uniform rod-like morphology AuPt-MB nano combined
Material, and show significant electro-chemical activity.In the present invention by AuPt-MB nano composite materials and Gal-3 specificity
Detect antibody A b2 to combine, and make sealer with BSA and novel nano signal probe is prepared.
In order to further increase the sensitivity of this interlayer type immunosensor and stability.Introduce a kind of new nanometer
Composite nitrogen mix graphene nanobelt (N-GNRs)-ferrous metal organic frame (Fe-MOFs)-Jenner's grain of rice (AuNPs)-
(N-GNRs-Fe-MOFs@AuNPs) is used as sensor base decorative material.Metal-organic framework (MOFs) is a species zeolite
The crystalline, porous material of shape, they have larger surface area, flexible porosity, are easy to customize composition and avtive spot is more
The advantages that, as novel and multifunctional material, they are widely used for gas storage/separation, the field such as catalysis and sensing.The present invention
The Fe-MOFs of middle introducing has the characteristics that stability is good, specific surface area is big and hypotoxicity.Because it has very big specific surface area can
Increase its electric conductivity to combine substantial amounts of AuNPs nanoparticles on its surface by Au-N chemical bonds, while contribute to lower step immobilized
With reference to Gal-3 specificity capture antibody As b1.In order to further increase electric conductivity, N-GNRs is mixed instead with Fe-MOFs@AuNPs
N-GNRs-Fe-MOFs AuNPs nano composite materials should be obtained.N-GNRs improves the conduction on modified electrode surface by being widely used in
N doping multi-walled carbon nanotubes (N-MWCNTs) synthesis of performance.Compared with CNT, N-GNR has reactive border and consolidated
Some terraced fields and hierarchic structure, chemism and adsorption capacity can be strengthened.Make us very surprised thing, last N-GNRs-Fe-
The advantages of MOFs@AuNPs nano composite materials not only have N-GNRs, Fe-MOFs and AuNPs, show also in terms of electric conductivity
Significant comprehensive effect is shown.Therefore, the novel nanocomposite materials are applied to the substrate modification of this electrochemical immunosensor
To further enhance the sensitivity of sensor and stability.
The present invention is based on AuPt-MB nano composite materials structure novel nano signal probe and N-GNRs-Fe-MOFs@
Substrate decorative material of the AuNPs nano composite materials as immunosensor, establish a kind of for galectin-3 in serum
(Gal-3) the electrochemical sensor preparation method of Concentration Testing and application, it is early to trigger the insulin resistance of diabetes B to provide
The diagnosis basis of phase.
The content of the invention：
It is an object of the invention to provide a kind of electrochemical sensing for being used for galectin-3 (Gal-3) Concentration Testing in serum
Device preparation method and application, it is characterised in that comprise the following steps：
(1) nitrogen mixes graphene nanobelt (N-GNRs)-ferrous metal organic frame (Fe-MOFs)-Jenner's grain of rice (AuNPs)
Sensor base modifies the preparation of nano composite material.
(2) golden platinum bimetallic (AuPt)-methylenum careuleum (MB)-Gal-3 specific detection antibodies (Ab2) nanowire signal probe
Prepare.
(3) electro-chemistry immunity biology sensor is established, detects Gal-3 in serum, draws standard curve.
The preparation process of N-GNRs-Fe-MOFs@AuNPs compounds of the present invention specifically includes following steps, its feature
It is to comprise the following steps：
(1) preparation of N-GNRs materials：
Weigh 0.1g nitrogen and mix multi-walled carbon nanotube (N-MWCNTs) addition to 10mL H2SO4And H3PO4In mixed solution
(H2SO4∶H3PO4=9: 1) mix.Above-mentioned mixed solution is placed in autoclave 140 are heated under the conditions of magnetic agitation
DEG C continue 10min, then reaction mixture is transferred in 65 DEG C of oil baths and adds 0.25g KMnO under agitation4Continue anti-
Answer 8min.Control solution is cooled to room temperature after reaction terminates, and resulting solution centrifuges 5min through 10000r/min, clear with ultra-pure water
Wash 3 times.After gained precipitation is placed in into vacuum drying 24h, 4 DEG C save backup.
(2) preparation of Fe-MOFs materials
0.187g FeCl are weighed respectively3·6H2O and 0.126g 2- amino terephthalic acid (TPA) adds 15mL dimethyl methyl
In acid amides (DMF) solution, mix.Above-mentioned mixed solution is placed in 120 DEG C of silicone oil and heats 4h, in 15min after beginning to warm up,
200 μ L glacial acetic acid is added into mixed solution.Control solution is cooled to room temperature after heating terminates, and resulting solution is through 10000r/
Min, 5min is centrifuged, is respectively cleaned 3 times with DMF, absolute ethyl alcohol, ultra-pure water respectively.After gained precipitation is placed in into vacuum drying 24h, 4
DEG C save backup.
(3) preparation of Fe-MOFs@AuNPs nano composite materials
Take 2mL HAuCl4·4H2O (1%) solution is added to 2mLFe-MOFs (the 1mg mL prepared-1) in solution, it is acute
After strong ultrasonic 30 minutes, 4mL NaBH are slowly added to dropwise into above-mentioned solution4(0.1M) while to be placed on magnetic stirring apparatus enterprising
Row stirring reaction 30min, resulting solution centrifuge 5min, cleaned 3 times with ultra-pure water through 10000r/min.Gained precipitation is divided again
It is dissipated in 4mL deionized water, 4 DEG C save backup.
(4) preparation of N-GNRs-Fe-MOFs@AuNPs nano composite materials
0.2mg N-GNRs ultrasonic dissolutions are weighed in 2mL deionized water, it is compound with 4mL Fe-MOFs@AuNPs
Thing solution ultrasound mixes 30min, under the conditions of magnetic agitation 500r/min, is stirred overnight.Resulting solution through 8000r/min, from
Heart 5min, cleaned 3 times with ultra-pure water.Gained is precipitated into redisperse into 4mL deionized water, 4 DEG C save backup.
AuPt-MB-Ab of the present invention2The preparation process of nanowire signal probe specifically includes following steps, and its feature exists
In comprising the following steps：
(1) preparation of AuPt-MB nano composite materials：
600 μ L HCL (0.1M) and 6mL DTABs (DTAB 1.14mM) is taken to add to 1mL respectively
MB (9.8mM) solution in, be stirred vigorously 10min.Add 100 μ L HAuCl simultaneously again4(4wt%) and 100 μ L H2PtCl6
(4wt%), is persistently stirred at room temperature 6h.Resulting solution centrifuges 10min, cleaned 3 times with ultra-pure water through 8000r/min.By gained
Redisperse is precipitated into 2mLPBS cushioning liquid (0.1M pH 7.0), 4 DEG C save backup.
(2)AuPt-MB-Ab2The preparation of nanowire signal probe：
Take 100 μ L Gal-3 specific detection antibodies Ab2Add into 2mL AuPt-MB Nanocomposite solutions, 4
Gentle agitation under the conditions of DEG C, continue 12h.40 μ L bovine serum albumin(BSA)s BSA (1wt%) are added to continue gently to stir under the conditions of 4 DEG C
Mix 6h.Resulting solution centrifuges 10min, gained is precipitated into redisperse to 2mLPBS cushioning liquid (0.1M pH through 8000r/min
7.0) in, 4 DEG C save backup.
It is of the present invention to establish electro-chemistry immunity biology sensor, Gal-3 in serum is detected, draws standard curve, its
It is characterised by comprising the following steps：
(1) respectively with 0.3 and 0.05 μm of Al2O3Powder by polishing electrode into minute surface, then respectively by ultra-pure water, anhydrous
Order each 5min of ultrasound electrode of ethanol, ultra-pure water, drying at room temperature are standby；
(2) the N-GNRs-Fe-MOFs@AuNPs Nanocomposite solutions prepared by 6 μ L are added dropwise in electrode surface, room
Temperature is dried.
(3) 6 μ L Gal-3 specificity capture antibody As b is added dropwise1For solution to the electrode surface being modified, 4 DEG C are incubated 12h.
(4) it is with distilled water that the electrode washing after incubation is clean, 6 μ L BSA (1wt%) sealers are added dropwise in electrode surface,
Room temperature closes 30min.
(5) electrode cleaning buffer solution (the 10mM Na after above-mentioned BSA is closed2HPO4, 2mM KH2PO4, 37mM
NaCl, 2.7mM KCl, pH 7.4) rinse well and dried in nitrogen.
(6) the target Gal-3 of 6 μ L various concentrations is added dropwise to electrode surface, 37 DEG C are incubated 1h and are rinsed with cleaning buffer solution
It is clean and dry in nitrogen.
(7) AuPt-MB-Ab prepared by 10 μ L is added dropwise in electrode surface after the drying2Nanowire signal probe solution, 37 DEG C incubate
1h is educated to be rinsed well with cleaning buffer solution and dried in nitrogen.
(8) electrode is placed in 5mL, characterized in 0.1M PBS (PH 7.0), measure its curent change current-responsive peak
Value.
(9) it is linear according to gained curent change current-responsive peak value and Gal-3 concentration, drawing curve.
Compared with prior art, one kind of the invention is used for the electrification of galectin-3 (Gal-3) Concentration Testing in serum
Transducer production method and application are learned, the characteristics of it is protruded is：
(1) N-GNRs-Fe-MOFs@AuNPs nano composite materials are repaiied for electro-chemistry immunity biosensor substrate
Decorations, provide not only the immobilized capture antibody of substantial amounts of avtive spot, and promote electronics conduction velocity, and then improve electrification
Learn sensitivity and the biocompatibility of immunity biosensor；
(2) the nano composite material structure signal probe based on AuPt-MB is incorporated into electro-chemistry immunity biology sensor
Preparation in, wherein AuPt bimetal nanos material not only have amplify its electrochemical signals function, and add its to inspection
Survey the biocompatibility of antibody.
(3) electro-chemistry immunity biology sensor prepared by this method can be the Gal-3 blood for causing insulin sensitivity to reduce
The detection of liquid concentration level provides new approaches, and early diagnosis foundation is provided for the prevention of diabetes B.
(4) identical nano material and method of modifying are used, using capturing antibody, signal probe and target protein
Specific recognition, only multiple protein need to can be achieved by the detection antibody for changing probe and the capture antibody for being immobilized on substrate and give birth to
The specificity of thing mark, highly sensitive detection, in addition, the method is easy, quickly, commercialization is easy to implement, so as to promote conversion to cure
Development.
Brief description of the drawings：
Fig. 1 is the structure schematic diagram of the electro-chemistry immunity biology sensor of the present invention.
Fig. 2 is the N-GNRs, Fe-MOFs, Fe-MOFs@AuNPs and N-GNRs-Fe-MOFs@AuNPs of present invention scanning
Electron microscope, AuPt-MB transmission electrograph mirror and EDS figure.
Fig. 3 schemes for Fe-MOFs@AuNPs EDS
Fig. 4 is the differential pulse voltametry that the electro-chemistry immunity biology sensor of the present invention obtains when detecting Gal-3
The linear relationship of current-responsive peak value and log concentration.
Fig. 5 is repeatability, specificity and the stability of sensor.
Embodiment：
The present invention is further elaborated with reference to specific embodiment, it should be appreciated that these embodiments are merely to illustrate
The present invention rather than limitation the scope of the present invention.
Embodiment 1
Step 1. weighs 0.187g FeCl respectively3·6H2O and 0.126g 2- amino terephthalic acid (TPA) adds the two of 15mL
In NMF (DMF) solution, mix.Above-mentioned mixed solution is placed in 120 DEG C of silicone oil and heats 4h, after beginning to warm up
During 15min, 200 μ L glacial acetic acid is added into mixed solution.Control solution is cooled to room temperature, resulting solution warp after heating terminates
10000r/min, 5min is centrifuged, is respectively cleaned 3 times with DMF, absolute ethyl alcohol, ultra-pure water respectively.Gained precipitation is placed in vacuum drying
After 24h, Fe-MOFs is obtained, 4 DEG C save backup.
Step 2. takes 2mL HAuCl4·4H2O (1%) solution is added to 2mL Fe-MOFs (the 1mg mL prepared-1) molten
In liquid, acutely ultrasound is slowly added to 4mL NaBH into above-mentioned solution dropwise after 30 minutes4(0.1M) while it is placed on magnetic agitation
Stirring reaction 30min is carried out on device, resulting solution centrifuges 5min, cleaned 3 times with ultra-pure water through 10000r/min.Obtain Fe-
MOFs@AuNPs, by gained Fe-MOFs@AuNPs sediments redisperses into 4mL deionized water, 4 DEG C save backup.
Step 3. weighs 0.2mg N-GNRs ultrasonic dissolutions in 2mL deionized water, by itself and 4mL Fe-MOFs@
AuNPs complex solutions ultrasound mixes 30min, under the conditions of magnetic agitation 500r/min, is stirred overnight.Resulting solution passes through
8000r/min, 5min is centrifuged, is cleaned 3 times with ultra-pure water.N-GNRs-Fe-MOFs@AuNPs are obtained, by gained N-GNRs-Fe-
Into 4mL deionized water, 4 DEG C save backup MOFs@AuNPs sediments redisperses.
Step 4. takes 600 μ L HCL (0.1M) and 6mL DTABs (DTAB 1.14mM) to add respectively
Into 1mL MB (9.8mM) solution, 10min is stirred vigorously.Add 100 μ L HAuCl simultaneously again4(4wt%) and 100 μ L
H2PtCl6(4wt%), is persistently stirred at room temperature 6h.Resulting solution centrifuges 10min, cleaned 3 times with ultra-pure water through 8000r/min.
By gained AuPt-MB sediments redisperse into 2mL PBS cushioning liquid (0.1M pH 7.0), 4 DEG C save backup.
Step 5. takes 100 μ L Gal-3 specific detection antibodies Ab2Add to 2mLAuPt-MB Nanocomposite solutions
In, the gentle agitation under the conditions of 4 DEG C, continue 12h.40 μ L bovine serum albumin(BSA)s BSA (1wt%) are added under the conditions of 4 DEG C to continue
Gentle agitation 6h.Resulting solution centrifuges 10min, obtains AuPt-MB-Ab through 8000r/min2Signal probe, gained signal is visited
Into 2mL PBS cushioning liquid (0.1M pH 7.0), 4 DEG C save backup pin sediment redisperse.
Step 6. is respectively with 0.3 and 0.05 μm of Al2O3Polishing electrode into minute surface, is then pressed ultra-pure water, nothing by powder respectively
Order each 5min of ultrasound electrode of water-ethanol, ultra-pure water, drying at room temperature are standby；
N-GNRs-Fe-MOFs@AuNPs Nanocomposite solutions prepared by 6 μ L are added dropwise in electrode table step 7.
Face, drying at room temperature.
6 μ L Gal-3 specificity capture antibody As b is added dropwise in step 8.1To the electrode surface being modified, 4 DEG C are incubated solution
12h。
Step 9. is clean by the electrode washing after incubation with distilled water, and 6 μ LBSA (1wt%) closings are added dropwise in electrode surface
Agent, room temperature closing 30min.
Step 10. by above-mentioned BSA close after electrode cleaning buffer solution (10mM Na2HPO4, 2mM KH2PO4, 37mM
NaCl, 2.7mM KCl, pH 7.4) rinse well and dried in nitrogen.
The target Gal-3 of 6 μ L various concentrations is added dropwise to electrode surface, 37 DEG C of incubation 1h cleaning buffer solutions by step 11.
Rinse well and dried in nitrogen.
The AuPt-MB-Ab prepared by 10 μ L is added dropwise in electrode surface to step 12. after the drying2Nanowire signal probe solution, 37
DEG C be incubated 1h rinsed well with cleaning buffer solution and in nitrogen dry.
Electrode is placed in 5mL by step 13., is characterized in 0.1M PBS (PH 7.0), measures its curent change electric current sound
Answer peak value.
Step 14. is linear according to gained curent change current-responsive peak value and Gal-3 concentration, and drawing is bent
Line.Measurement result shows Gal-3 concentration in 100fg mL-1-50ng mL-1In the range of it is linear, linearly dependent coefficient is
0.99502, detection is limited to 33.33fg mL-1(S/N=3).
The sensor of the present invention is used differential pulse voltametry detection sensor electric current by step 15. in 4 DEG C of preservations, interruption
Response, current-responsive is still the 95.17% of initial current after storing 3 weeks, and surface probe has good stability；
Step 16. present invention takes immunity biosensor 5 prepared by same batch, under the same conditions to 1ng mL-1
Gal-3 be measured respectively, each determination of electrode 3 times, as a result the relative standard deviation of current-responsive value is 2.75%, is said
The repeatability of bright sensor is good.
The sensor of the present invention is used to detect 100ng mL by step 17.-1Disturbance thing and 1ng mL-1Gal-3's
Mixture, the relative standard deviation of its testing result is 1.55%, illustrates chaff interference to sensor detection results interference effect very
It is small, sensor it is specific good.
Described above is only the preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, under the precondition for not departing from the principle of the invention, some improvements and modifications can also be made, these improve and
Retouching also should be regarded as protection scope of the present invention.
Claims (4)
1. one kind is used for the electrochemical sensor preparation method of galectin-3 (Gal-3) Concentration Testing in serum, its feature exists
In comprising the following steps：
(1) nitrogen mixes graphene nanobelt (N-GNRs)-ferrous metal organic frame (Fe-MOFs)-Jenner's grain of rice (AuNPs) sensing
Device substrate modifies the preparation of nano composite material.
(2) golden platinum bimetallic (AuPt)-methylenum careuleum (MB)-Gal-3 specific detection antibodies (Ab2) nanowire signal probe preparation.
(3) electro-chemistry immunity biology sensor is established, detects Gal-3 in serum, draws standard curve.
2. the preparation process of N-GNRs-Fe-MOFs@AuNPs compounds specifically includes following steps according to claim 1,
It is characterized in that comprise the following steps：
(1) preparation of N-GNRs materials：
Weigh 0.1g nitrogen and mix multi-walled carbon nanotube (N-MWCNTs) addition to 10mL H2SO4And H3PO4In mixed solution
(H2SO4∶H3PO4=9: 1) mix.Above-mentioned mixed solution is placed in autoclave 140 are heated under the conditions of magnetic agitation
DEG C continue 10min, then reaction mixture is transferred in 65 DEG C of oil baths and adds 0.25g KMnO under agitation4Continue anti-
Answer 8min.Control solution is cooled to room temperature after reaction terminates, and resulting solution centrifuges 5min through 10000r/min, clear with ultra-pure water
Wash 3 times.After gained precipitation is placed in into vacuum drying 24h, 4 DEG C save backup.
(2) preparation of Fe-MOFs materials
0.187g FeCl are weighed respectively3·6H2O and 0.126g 2- amino terephthalic acid (TPA) adds 15mL dimethylformamide
(DMF) in solution, mix.Above-mentioned mixed solution is placed in 120 DEG C of silicone oil and heats 4h, in 15min after beginning to warm up, to mixed
Close the glacial acetic acid that 200 μ L are added in solution.Control solution is cooled to room temperature after heating terminates, resulting solution through 10000r/min,
5min is centrifuged, is respectively cleaned 3 times with DMF, absolute ethyl alcohol, ultra-pure water respectively.After gained precipitation is placed in into vacuum drying 24h, 4 DEG C of guarantors
Deposit standby.
(3) preparation of Fe-MOFs@AuNPs nano composite materials
Take 2mL HAuCl4·4H2O (1%) solution is added to 2mL Fe-MOFs (the 1mg mL prepared-1) in solution, it is acutely super
After sound 30 minutes, 4mL NaBH are slowly added to dropwise into above-mentioned solution4(0.1M) while it is placed on magnetic stirring apparatus and is stirred
Reaction 30min is mixed, resulting solution centrifuges 5min, cleaned 3 times with ultra-pure water through 10000r/min.By gained precipitation redisperse extremely
In 4mL deionized water, 4 DEG C save backup.
(4) preparation of N-GNRs-Fe-MOFs@AuNPs nano composite materials
0.2mg N-GNRs ultrasonic dissolutions are weighed in 2mL deionized water, it is molten with 4mL Fe-MOFs@AuNPs compounds
Liquid ultrasound mixes 30min, under the conditions of magnetic agitation 500r/min, is stirred overnight.Resulting solution is through 8000r/min, centrifugation
5min, cleaned 3 times with ultra-pure water.Gained is precipitated into redisperse into 4mL deionized water, 4 DEG C save backup.
3. AuPt-MB-Ab according to claim 12The preparation process of nanowire signal probe specifically includes following steps, and it is special
Sign is to comprise the following steps：
(1) preparation of AuPt-MB nano composite materials：
600 μ L HCL (0.1M) and 6mL DTABs (DTAB 1.14mM) is taken to add to 1mL MB respectively
In (9.8mM) solution, 10min is stirred vigorously.Add 100 μ L HAuCl simultaneously again4(4wt%) and 100 μ L H2PtCl6
(4wt%), is persistently stirred at room temperature 6h.Resulting solution centrifuges 10min, cleaned 3 times with ultra-pure water through 8000r/min.By gained
Redisperse is precipitated into 2mLPBS cushioning liquid (0.1M pH7.0), 4 DEG C save backup.
(2)AuPt-MB-Ab2The preparation of nanowire signal probe：
Take 100 μ L Gal-3 specific detection antibodies Ab2Add into 2mL AuPt-MB Nanocomposite solutions, in 4 DEG C of bars
Gentle agitation under part, continue 12h.Add 40 μ L bovine serum albumin(BSA)s BSA (1wt%) and continue gentle agitation 6h under the conditions of 4 DEG C.
Resulting solution centrifuges 10min, gained is precipitated into redisperse to 2mL PBS cushioning liquid (0.1M pH7.0) through 8000r/min
In, 4 DEG C save backup.
4. according to claim 1 establish electro-chemistry immunity biology sensor, Gal-3 in serum is detected, it is bent to draw standard
Line, it is characterised in that comprise the following steps：
(1) respectively with 0.3 and 0.05 μm of Al2O3Powder by polishing electrode into minute surface, then respectively by ultra-pure water, absolute ethyl alcohol,
Order each 5min of ultrasound electrode of ultra-pure water, drying at room temperature are standby；
(2) the N-GNRs-Fe-MOFs@AuNPs Nanocomposite solutions prepared by 6 μ L are added dropwise and done in electrode surface, room temperature
It is dry.
(3) 6 μ L Gal-3 specificity capture antibody As b is added dropwise1For solution to the electrode surface being modified, 4 DEG C are incubated 12h.
(4) it is with distilled water that the electrode washing after incubation is clean, 6 μ L BSA (1wt%) sealers, room temperature is added dropwise in electrode surface
Close 30min.
(5) electrode cleaning buffer solution (the 10mM Na after above-mentioned BSA is closed2HPO4, 2mM KH2PO4, 37mM NaCl,
2.7mM KCl, pH7.4) rinse well and dried in nitrogen.
(6) the target Gal-3 of 6 μ L various concentrations is added dropwise to electrode surface, 37 DEG C are incubated 1h and are rinsed well with cleaning buffer solution
And dried in nitrogen.
(7) the AuPt-MB-Ab2 nanowire signal probe solutions prepared by 10 μ L, 37 DEG C of incubation 1h are added dropwise in electrode surface after the drying
Rinsed well with cleaning buffer solution and dried in nitrogen.
(8) electrode is placed in 5mL, characterized in 0.1M PBS (PH7.0), measure its curent change current-responsive peak value.
(9) it is linear according to gained curent change current-responsive peak value and Gal-3 concentration, drawing curve.
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