CN112452762A - Aerosol particle sorting system and method - Google Patents
Aerosol particle sorting system and method Download PDFInfo
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- CN112452762A CN112452762A CN202011412837.9A CN202011412837A CN112452762A CN 112452762 A CN112452762 A CN 112452762A CN 202011412837 A CN202011412837 A CN 202011412837A CN 112452762 A CN112452762 A CN 112452762A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B9/00—Combinations of apparatus for screening or sifting or for separating solids from solids using gas currents; General arrangement of plant, e.g. flow sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B11/00—Arrangement of accessories in apparatus for separating solids from solids using gas currents
- B07B11/04—Control arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B11/00—Arrangement of accessories in apparatus for separating solids from solids using gas currents
- B07B11/06—Feeding or discharging arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
- G01N2001/387—Other diluting or mixing processes mixing by blowing a gas, bubbling
Abstract
The invention discloses an aerosol particle sorting system and method, which comprises the following steps: the driving device is used for injecting the auxiliary gas and the aerosol sample into the screening device at a set speed ratio; the screening device is used for sorting the aerosol samples; wherein the screening device comprises: the microfluidic chip is at least provided with two channels which are mutually crossed and used for shunting the injected aerosol sample; the chip clamp is used for clamping the microfluidic chip; the collection cavity is positioned below the microfluidic chip and is used for collecting particles in the aerosol sample; and injecting an aerosol sample and auxiliary gas through a driving device, and driving the aerosol sample to pass through the first channel in the microfluidic chip one by one in a single-particle form to enter the collection cavity. The invention can realize the particle sorting requirement of the aerosol of one or more particles, greatly improve the sorting efficiency, improve the sorting precision, reduce the complexity of equipment and simplify the operation flow.
Description
Technical Field
The invention relates to the technical field of aerosol particle sorting, in particular to an aerosol particle sorting system and method.
Background
Aerosol, such as smoke, PM2.5(Particulate matter2.5, particles with a particle size of 2.5 microns or less in air), etc., when analyzing particles in a colloid, a complex process is often required, single particles in the aerosol are not easy to capture, and therefore, the characteristics of the single particles are difficult to obtain accurately, and at this time, a method for conveniently and efficiently sorting the same kind or multiple kinds of particles in the aerosol is required to separate and capture the single particles, which is called aerosol particle sorting.
Currently, aerosol particle sorting methods used include a screening method, a sedimentation method, an electrophoresis method (DEP), and the like, but the sorting methods cannot well realize sorting and capturing due to the defects that used instruments are complex and expensive, and are not suitable for all micro-nano particles, and the like, and unnecessary sample waste is easily caused.
Disclosure of Invention
In view of the above problems, the present invention provides a system and a method for sorting aerosol particles, so as to accurately and efficiently sort aerosol particles, and overcome the defects of the existing sorting method.
In order to achieve the purpose, the invention adopts the following technical scheme:
one aspect of the present invention provides an aerosol particle sorting system comprising:
the driving device is used for injecting the auxiliary gas and the aerosol sample into the screening device at a set speed ratio;
the screening device is used for sorting the aerosol samples;
wherein the screening device comprises:
the microfluidic chip is at least provided with two channels which are mutually crossed and used for shunting the injected aerosol sample;
the chip clamp is used for clamping the microfluidic chip;
the collection cavity is positioned below the microfluidic chip and is used for collecting particles in the aerosol sample;
and injecting an aerosol sample and auxiliary gas through a driving device, and driving the aerosol sample to pass through the first channel in the microfluidic chip one by one in a single-particle form to enter the collection cavity.
Preferably, the driving means includes: first drive unit, second drive unit and temperature control unit, wherein, first drive unit with the temperature control unit is connected, first drive unit through advance the appearance pipe with the sample cell of micro-fluidic chip is connected, is used for the drive the aerosol sample is followed the one end of first passageway is injected into in the micro-fluidic chip, the temperature control unit set up in the sample cell department of micro-fluidic chip is used for controlling the temperature of aerosol sample, the second drive unit is used for driving supplementary gas is injected from the passageway outside the first passageway in the micro-fluidic chip, wherein, the other end of first passageway through go out the appearance pipe with collect the chamber and connect.
Preferably, the microfluidic chip is cross-shaped and has a first channel and a second channel which are perpendicular to each other.
Preferably, the driving device further comprises a third driving unit, and the third driving unit and the second driving unit drive the auxiliary gas to be injected into the microfluidic chip from two ends of the second channel respectively.
Preferably, the first driving unit, the second driving unit, and the third driving unit are all mounted at a bottom end of the chip gripper.
Preferably, a plurality of micropore units with set sizes are arranged in the collection cavity, and the micropore units are arranged in a staggered array.
In order to achieve the above object, another aspect of the present invention provides an aerosol particle sorting method, comprising the steps of:
mounting a microfluidic chip on a chip clamp, wherein the microfluidic chip has at least two channels which are intersected with each other;
setting the injection speed ratio of the auxiliary gas and the aerosol sample through a driving device;
and injecting the aerosol sample into the microfluidic chip from one end of the first channel according to a set speed ratio, and injecting the auxiliary gas into the microfluidic chip from a channel except the first channel, so that the aerosol sample passes through the first channel of the microfluidic chip one by one in a single particle form and enters the collection cavity.
Preferably, before injecting the aerosol sample into the microfluidic chip, the method further comprises:
and heating the aerosol sample to be injected through a temperature control unit, wherein the temperature control unit is arranged at a sample pool of the microfluidic chip.
Preferably, the method further comprises the following steps: aerosol particles are sorted and collected by a plurality of micropore units with set sizes arranged in the collection cavity.
Preferably, before injecting the aerosol sample and the auxiliary gas into the microfluidic chip, the method further comprises: and introducing auxiliary gas to clean and conduct the interior of the microfluidic chip.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the invention adopts micro-fluid focusing to reshape and accelerate the aerosol sample, thereby greatly improving the sorting efficiency; by adopting the micropore array, particles meeting the particle size can pass through, the particles are further screened, and the screening precision is improved.
The invention uses external mechanical drive and internal temperature control drive, breaks the singleness of the drive mode, and effectively improves the screening rate by increasing the internal energy of particles;
the invention adopts an internal temperature control system, utilizes the compressibility of gas and the temperature rise and expansion of the aerosol sample to achieve the dilution effect on the sample and simplify the operation process.
The invention realizes different particle focusing widths by adjusting the velocity ratio of the auxiliary gas to the aerosol sample gas, and has wide range of applicable particle size.
Drawings
FIG. 1 is a schematic diagram of the aerosol particle sorting system of the present invention;
FIG. 2 is a schematic diagram of an aerosol sample and an auxiliary gas injected into a microfluidic chip according to the present invention;
FIG. 3 is a graph of assist gas to aerosol sample velocity ratio and focal width in accordance with the present invention;
fig. 4 is a schematic flow diagram of a method of sorting aerosol particles according to the present invention.
Detailed Description
The embodiments of the present invention will be described below with reference to the accompanying drawings. Those of ordinary skill in the art will recognize that the described embodiments can be modified in various different ways, or combinations thereof, without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and not intended to limit the scope of the claims. Furthermore, in the present description, the drawings are not to scale and like reference numerals refer to like parts.
Fig. 1 is a schematic diagram of the aerosol particle sorting system according to the present invention, and as shown in fig. 1, the aerosol particle sorting system includes: the device comprises a driving device and a screening device, wherein the driving device is used for injecting auxiliary gas and an aerosol sample into the screening device at a set speed ratio, the auxiliary gas is clean gas without impurities and does not react with particles in the aerosol sample, and the auxiliary gas is pure rare gas, nitrogen and the like; the screening device is used for sorting the aerosol samples; wherein the screening device comprises: the micro-fluidic chip 2 is at least provided with two channels which are mutually crossed and used for shunting and shaping the injected aerosol sample; the chip clamp 3 is used for clamping the microfluidic chip 2; the collection cavity 4 is positioned below the microfluidic chip 2 and is used for collecting particles in the aerosol sample; as shown in fig. 2, the driving device injects the auxiliary gas and the aerosol sample at a set velocity ratio to drive the aerosol sample to pass through the first channel 21 in the microfluidic chip 2 one by one in a single particle form into the collection chamber 4. Because the auxiliary gas does not react with the aerosol sample gas, when the auxiliary gas and the aerosol sample gas are both injected into the microfluidic chip 2, the auxiliary gas can extrude the aerosol sample gas, so that when the focusing width of the aerosol sample (namely the width of the aerosol sample gas in the first channel 21) is 1-2 times of the particle size of the particles to be collected, the particles in the aerosol can sequentially pass through the first channel 21 in the microfluidic chip 2 one by one and enter the collection cavity 4, and the separation of the aerosol particles is realized.
The invention uses the micro-fluidic chip 2 to sort the aerosol particles, has the advantages of less sample consumption, simple structure, short time and the like, can well meet the requirement of efficiently sorting the particles, and particularly can sort out the particles without special properties for the insulating particles; and because the driving device has controllability on aerosol samples and auxiliary gas, aerosol particles with different particle sizes can be easily separated, namely 1nm to 10 nm6The particles with the size of nm can be screened and captured; when the particle sizes of the aerosol are various, the particles with various sizes can be sorted by utilizing the plasticity of the microfluidic chip 2.
In one embodiment, the driving device includes: first drive unit 12, second drive unit 13 and temperature control unit 11, wherein, first drive unit 12 with temperature control unit 11 connects, first drive unit 12 through advance the appearance pipe with the sample cell of micro-fluidic chip 2 is connected, is used for the drive the aerosol sample is followed the one end of first passageway 21 is injected in micro-fluidic chip 2, temperature control unit 11 set up in the sample cell department of micro-fluidic chip 2 is used for control the temperature of aerosol sample provides interior energy for the aerosol sample, second drive unit 13 is used for the drive auxiliary gas is injected from the passageway outside first passageway 21 in micro-fluidic chip 2, wherein, the other end of first passageway 21 through go out the appearance pipe with collect the chamber 4 and be connected. The temperature control unit 11 is arranged inside the microfluidic chip 2, specifically, arranged at a sample cell of the microfluidic chip 2, the temperature control unit 11 can be a small integrated intelligent controller, the temperature of the aerosol sample is controlled to be about 45 ℃, the aerosol sample is heated at an inlet of the microfluidic chip 2, and the aerosol sample can expand due to the compressibility of gas, so that the sample gas can be diluted, and the dilution of 1-2 times can be generally realized due to the limited volume of a sample inlet and the limited heating temperature. Meanwhile, the heated heat energy provides power for injecting aerosol sample particles into the microfluidic chip 2, so that the aerosol particle particles have kinetic energy, and the aerosol sample can be driven to be injected into the microfluidic chip 2 together with the external first driving unit 12, the singleness of a driving mode is broken, and the separation efficiency is effectively improved by increasing the internal energy of the particles. And the temperature control unit 11 is arranged at the sample cell of the microfluidic chip 2, and achieves the effect of diluting the sample through temperature rise and expansion, thereby simplifying the operation process.
As shown in fig. 2, the microfluidic chip 2 has a cross shape and has a first channel 21 and a second channel 22 perpendicular to each other. The first channel 21 is a main flow channel of an aerosol sample, one end of the first channel 21 is connected with the sample cell through a sample inlet pipe, and the other end of the first channel 21 is connected with the collection cavity 4 through a sample outlet pipe; the second channel 22 is perpendicular to the first channel 21, both ends of the second channel 22 are used for injecting auxiliary gas, the auxiliary gas injected from both ends of the second channel 22 flows towards the first channel 21 and generates an extrusion effect on the aerosol sample in the first channel 21, so that the aerosol sample gradually narrows from the original inlet width after entering the microfluidic chip 2, and aerosol particles enter the collection cavity 4 through the first channel 21 one by one and orderly in a single particle form by setting a speed ratio.
The second driving unit 13 can simultaneously drive the auxiliary gas to enter the microfluidic chip 2 from two ends of the second channel 22, and optionally, the driving apparatus further includes a third driving unit 14, and the third driving unit 14 and the second driving unit 13 drive the auxiliary gas to be injected into the microfluidic chip 2 from two ends of the second channel 22, respectively.
In an alternative embodiment, the first driving unit 12, the second driving unit 13 and the third driving unit 14 are all mounted at the bottom end of the chip gripper 3, for example, they may be mounted as one body by a gripper base embedding structure, simplifying the device configuration.
The first driving unit 12, the second driving unit 13, and the third driving unit 14 are external mechanical driving gas devices, have a precision of 0.01uL/s, and may be a syringe pump, an air pump, or the like. When the speed ratio of the auxiliary gas and the aerosol sample is set by the driving device, the setting can be realized by setting parameters of the first driving unit 12, the second driving unit 13, and the third driving unit 14, for example, the setting parameters of the syringe pump include flow rate, duration, syringe size, and the like, and the setting parameters of the air pump include: air pressure, duration, etc. FIG. 3 is a graph of the velocity ratio of the assist gas to the aerosol sample and the focusing width in accordance with the present invention, as shown in FIG. 3, at an aerosol sample density of 1.03g/cm3For example, the speed ratio (sheath sample speed ratio) and the focusing width are in one-to-one correspondence, and by setting the speed ratio, the size of the focusing width can be adjusted, so that the focusing width is 1-2 times of the particle size of the particles to be screened, only one particle can be accommodated, and the single particle can enter the collection cavity 4 after passing through the focusing region one by one (the focusing region is an aerosol sample flowing region formed after the auxiliary gas extrusion). The focusing width refers to a flow width of the aerosol sample in the first channel 21, and specifically, the flow width of the aerosol sample is formed after the aerosol sample and the auxiliary gas are converged at a cross of the microfluidic chip 2 and are squeezed by the auxiliary gas.
In the present invention, it can be determined that aerosol particles are in the form of single particles, which pass through the first passage 21 one by one into the collection chamber 4, specifically, it includes: since the signal characteristics of the particle swarm and the single particle are different, the corresponding particle signals can be displayed through an integrated optical detection system; alternatively, it is directly observed by an integrated optical microscope. When the focusing width is 1-2 times of the particle size of the particles to be screened, the aerosol samples can be ensured to pass through one by one in a single particle mode.
In one embodiment, a plurality of micropore units 41 with a set size are arranged in the collection cavity 4, the micropore units 41 are arranged in a staggered array, and only particles meeting the set size can pass through the micropore units and enter the collection cavity 4, so that further accurate screening is realized, and the separation and capture process of aerosol particles is completed. Wherein, micropore unit 41 is the netted or cylindrical micropore structure of round hole, plays further screening, the collection effect to aerosol sample granule.
Fig. 4 is a schematic flow chart of the aerosol particle sorting method according to the present invention, and as shown in fig. 4, the aerosol particle sorting method according to the present invention includes the following steps:
step S1, mounting the microfluidic chip 2 on the chip holder 3, where the microfluidic chip 2 has at least two channels that intersect with each other, and regarding the specific structure of the chip holder 3, the present invention is not specifically limited, and among the channels of the microfluidic chip 2, the first channel 21 is used as a main flow channel of the aerosol sample, and the other channels are used as channels of the auxiliary gas;
step S2, setting the injection speed ratio of the auxiliary gas and the aerosol sample through a driving device, wherein the speed ratio is set on the basis that the flowing width of the aerosol sample in the first channel 21 is 1-2 times of the particle size of the particles to be screened;
step S3, injecting the aerosol sample into the microfluidic chip 2 from one end of the first channel 21 and injecting the auxiliary gas into the microfluidic chip 2 from a channel other than the first channel 21 at a set speed ratio, so that the aerosol sample passes through the first channel 21 of the microfluidic chip 2 one by one in a single particle form and enters the collection chamber 4.
It should be noted that different speed ratios correspond to different focusing widths, so that particle screening of different particle sizes can be realized, and the range of samples is wide. When screening multiple different-size particles in the same aerosol sample, different speed ratios can be set in sequence according to the order of the particle sizes of the particles to be screened from small to large, so that the particles with smaller particle sizes firstly pass through the focusing area in the first channel 21, and then the particles with larger particle sizes are selected, thereby sequentially screening the aerosol particles with different particle sizes.
In one embodiment, the driving device includes: a first drive unit 12 and a second drive unit 13, wherein the ratio of the injection speeds of the secondary gas and the aerosol sample is set by a drive device, comprising: the speed of the aerosol sample injected into the microfluidic chip 2 from one end of the first channel 21 is set by the first driving unit 12, and the speed of the auxiliary gas injected into the microfluidic chip 2 from a channel other than the first channel 21 is set by the second driving unit 13, wherein the other end of the first channel 21 is connected with the collection cavity 4 through a sample outlet pipe.
When the microfluidic chip 2 is cross-shaped, it has a first channel 21 and a second channel 22 perpendicular to each other. The first channel 21 is a main flow channel of an aerosol sample, one end of the first channel 21 is connected with the sample cell through a sample inlet pipe, and the other end of the first channel 21 is connected with the collection cavity 4 through a sample outlet pipe; the second channel 22 is perpendicular to the first channel 21, both ends of the second channel 22 are used for injecting auxiliary gas, and the auxiliary gas injected from both ends of the second channel 22 flows towards the first channel 21 and generates a squeezing effect on the aerosol sample in the first channel 21. The second driving unit 13 can simultaneously drive the auxiliary gas to enter the microfluidic chip 2 from two ends of the second channel 22, and optionally, the driving apparatus further includes a third driving unit 14, and the auxiliary gas is driven by the third driving unit 14 and the second driving unit 13 to be respectively injected into the microfluidic chip 2 from two ends of the second channel 22.
The first driving unit 12, the second driving unit 13, and the third driving unit 14 are external mechanical driving gas devices, have a precision of 0.01uL/s, and may be a syringe pump, an air pump, or the like. When the speed ratio of the auxiliary gas and the aerosol sample is set by the driving device, the setting can be realized by setting parameters of the first driving unit 12, the second driving unit 13, and the third driving unit 14, for example, the setting parameters of the syringe pump include flow rate, duration, syringe size, and the like, and the setting parameters of the air pump include: air pressure, duration, etc.
In one embodiment, the driving device further comprises a temperature control unit 11, and before the aerosol sample is injected into the microfluidic chip 2, the sorting method further comprises: the aerosol sample to be injected is heated by a temperature control unit 11, wherein the temperature control unit 11 is arranged at the sample cell of the microfluidic chip 2. The temperature control unit 11 is arranged inside the microfluidic chip 2, specifically, arranged at a sample cell of the microfluidic chip 2, the temperature control unit 11 can be a small integrated intelligent controller, the temperature of the aerosol sample is controlled to be about 45 ℃, the aerosol sample is heated at an inlet of the microfluidic chip 2, and the aerosol sample can expand due to the compressibility of gas, so that the sample gas can be diluted, and the dilution of 1-2 times can be generally realized due to the limited volume of a sample inlet and the limited heating temperature. Meanwhile, the heated heat energy provides power for injecting aerosol sample particles into the microfluidic chip 2, so that the aerosol particle particles have kinetic energy, and the aerosol sample can be driven to be injected into the microfluidic chip 2 together with the external first driving unit 12, the singleness of a driving mode is broken, and the separation efficiency is effectively improved by increasing the internal energy of the particles. And the temperature control unit 11 is arranged at the sample cell of the microfluidic chip 2, and achieves the effect of diluting the sample through temperature rise and expansion, thereby simplifying the operation process.
In one embodiment, the sorting method further comprises: aerosol particles are sorted and collected by a plurality of pore units 41 with set sizes arranged in the collection cavity 4. Wherein, a plurality of micropore units 41 are arranged in a staggered array, and only particles meeting the set size can pass through and enter the collection cavity 4, so that further accurate screening is realized, and the separation and capture process of aerosol particles is completed. Wherein, micropore unit 41 is the micropore structure of round hole form or cylindric, plays further screening effect to aerosol sample granule.
In one embodiment, before injecting the aerosol sample and the auxiliary gas into the microfluidic chip 2, the method further includes: and introducing auxiliary gas to clean and conduct the interior of the microfluidic chip 2.
The aerosol particle sorting process is described in detail below by taking the microfluidic chip 2 as a cross-shaped microfluidic chip 2 as an example.
Firstly, the microfluidic chip 2 is installed on a chip clamp 3, a first driving unit 12, a second driving unit 13 and a third driving unit 14 are installed on a base of the chip clamp 3, the microfluidic chip and the chip clamp can be integrally installed through a clamp base embedding structure, and a temperature control unit 11 is installed at a sample cell of the microfluidic chip 2;
then, according to the particle size of the particles to be screened, parameters of the first driving unit 12, the second driving unit 13 and the third driving unit 14 are set, so that the speed ratio of the auxiliary gas to the aerosol sample injected into the microfluidic chip 2 is set, and the focusing width of the aerosol sample in the first channel 21 is controlled, so that the focusing width is 1-2 times of the particle size of the particles to be screened.
Then, clean auxiliary gas is introduced to clean and conduct the internal structure of the microfluidic chip 2.
Then, starting the temperature control unit 11, introducing an aerosol sample, and controlling the temperature of the aerosol sample to be about 45 ℃ so that the aerosol sample is heated and expanded;
and finally, starting the first driving unit 12, the second driving unit 13 and the third driving unit 14 to drive the aerosol sample to be injected into the microfluidic chip 2 from one end of the first channel 21, respectively injecting auxiliary gas into the microfluidic chip 2 from two ends of the second channel 22, and forming an aerosol sample circulation area with a certain focusing width in the first channel 21 through the extrusion effect of the auxiliary gas, so that particles meeting the particle size pass through the focusing area and then enter the collection cavity 4, and the separation of the aerosol particles is completed.
The aerosol particle sorting system and the sorting method are suitable for aerosol particle sorting and related fields, the focusing width is controlled by setting the speed ratio of the auxiliary gas to the aerosol sample, the requirement of sorting aerosol particles containing one or more particles can be met, the sample range is wide, meanwhile, due to the focusing effect of microfluid, the aerosol sample can rapidly pass through the microfluidic chip 2 and finally enter the micropore unit 41 for further screening, the sorting efficiency is greatly improved, the sorting precision is improved, the equipment complexity is reduced, and the operation flow is simplified.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An aerosol particle sorting system, comprising:
the driving device is used for injecting the auxiliary gas and the aerosol sample into the screening device at a set speed ratio;
the screening device is used for sorting the aerosol samples;
wherein the screening device comprises:
the microfluidic chip is at least provided with two channels which are mutually crossed and used for shunting the injected aerosol sample;
the chip clamp is used for clamping the microfluidic chip;
the collection cavity is positioned below the microfluidic chip and is used for collecting particles in the aerosol sample;
and injecting an aerosol sample and auxiliary gas through a driving device, and driving the aerosol sample to pass through the first channel in the microfluidic chip one by one in a single-particle form to enter the collection cavity.
2. The aerosol particle sorting system of claim 1, wherein the drive device comprises: first drive unit, second drive unit and temperature control unit, wherein, first drive unit with the temperature control unit is connected, first drive unit through advance the appearance pipe with the sample cell of micro-fluidic chip is connected, is used for the drive the aerosol sample is followed the one end of first passageway is injected into in the micro-fluidic chip, the temperature control unit set up in the sample cell department of micro-fluidic chip is used for controlling the temperature of aerosol sample, the second drive unit is used for driving supplementary gas is injected from the passageway outside the first passageway in the micro-fluidic chip, wherein, the other end of first passageway through go out the appearance pipe with collect the chamber and connect.
3. The aerosol particle sorting system of claim 2, wherein the microfluidic chip is cross-shaped with a first channel and a second channel perpendicular to each other.
4. The aerosol particle sorting system according to claim 3, wherein the driving device further comprises a third driving unit, and the third driving unit and the second driving unit drive the auxiliary gas to be injected into the microfluidic chip from both ends of the second channel, respectively.
5. The aerosol particle sorting system of claim 4, wherein the first drive unit, the second drive unit, and the third drive unit are all mounted at a bottom end of the chip gripper.
6. The aerosol particle sorting system of claim 1, wherein the collection chamber has a plurality of micro-porous units of a predetermined size disposed therein, the plurality of micro-porous units being arranged in a staggered array.
7. A method of sorting aerosol particles, comprising the steps of:
mounting a microfluidic chip on a chip clamp, wherein the microfluidic chip has at least two channels which are intersected with each other;
setting the injection speed ratio of the auxiliary gas and the aerosol sample through a driving device;
and injecting the aerosol sample into the microfluidic chip from one end of the first channel according to a set speed ratio, and injecting the auxiliary gas into the microfluidic chip from a channel except the first channel, so that the aerosol sample passes through the first channel of the microfluidic chip one by one in a single particle form and enters the collection cavity.
8. The method of claim 7, further comprising, prior to injecting the aerosol sample into the microfluidic chip:
and heating the aerosol sample to be injected through a temperature control unit, wherein the temperature control unit is arranged at a sample pool of the microfluidic chip.
9. The aerosol particle sorting method of claim 7, further comprising: aerosol particles are sorted and collected by a plurality of micropore units with set sizes arranged in the collection cavity.
10. The method of claim 7, further comprising, prior to injecting the aerosol sample and the assist gas into the microfluidic chip: and introducing auxiliary gas to clean and conduct the interior of the microfluidic chip.
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