CN112438964A - Composite antibacterial film spraying agent of lysostaphin and chitosan - Google Patents
Composite antibacterial film spraying agent of lysostaphin and chitosan Download PDFInfo
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Abstract
The invention discloses a lysostaphin and chitosan composite antibacterial film spraying agent; the raw materials for preparing the composite gel comprise, by mass volume, 1-20% of lysostaphin, 0.1-5% of chitosan, 0.5-10% of a surface tension regulator, 0.5-5% of a pH regulator and the balance of medical purified water; the preparation method comprises the following preparation steps: s1, adjusting the pH value of the medical pure water under aseptic condition; s2, adding chitosan into the S1 mixed solution to form a solution; s3, adding lysostaphin into the solution in the S2; the composite antibacterial film spraying agent can form a film quickly, has long maintenance time, can stop bleeding and blood coagulation quickly, forms a water-insoluble protective film on a wound surface, has certain air permeability, has a wide antibacterial range, does not stimulate the skin, is added with a surface tension regulator, is helpful for forming the water-insoluble protective film, and is not easy to demould.
Description
Technical Field
The invention belongs to the technical field of biological preparations, and particularly relates to a lysostaphin and chitosan composite antibacterial film spraying agent.
Background
In life, people often suffer from medium and micro wounds, bleeding and swelling occur to wounds, and along with bacterial infection, people mostly adopt band-aid, iodophor or antibacterial and anti-inflammatory spray for treatment at present, but the defects of inconvenient nursing after treatment, poor wound healing capability, aggravated infection after exposure to water and the like exist. If the wound cannot be effectively protected and treated and the wound can cause secondary infection of wound bacteria, wound fester, septicemia and other adverse effects, staphylococcus aureus is the most main gram-positive pathogenic bacterium, and the gram-negative pathogenic bacterium inducing wound infection, such as pseudomonas aeruginosa, is a dangerous bacterium with drug resistance to various antibiotics. In recent years, along with the use of a large amount of antibiotics, the sensitivity of germs to the antibiotics is gradually reduced, common antibiotics are likely to have no effect when being used for treatment, the drug resistance problem of germs is increasingly prominent, and great harm is caused to the life health of human beings.
In recent years, film spray agents have attracted much attention. The spray film agent is also called spray film agent, combines the advantages of film agent and spray agent, is stored in a solution state, can be sprayed in a mist form to quickly form a biological film with good air permeability on the wound surface, and has wide development space in the aspect of external preparations. The usual components include film-forming materials, solvents and some transdermal enhancers. However, the common film spraying agent has a problem that a film formed on the surface of a wound after a liquid medicine is sprayed cannot be well attached to the wound due to the influence of surface tension, so that a film releasing phenomenon sometimes occurs, and secondary infection of the wound is caused.
The antibacterial peptide spray film-forming agent and the preparation method thereof, which are disclosed in the publication No. CN1899600B, can form a film within a few minutes after the antibacterial peptide spray film-forming agent is sprayed on an affected part, not only have a direct protection effect on the affected part, rapidly kill bacteria and prevent the affected part from further infection, but also have good affinity and air permeability to skin; the spraying film forming agent contains a humectant component, can absorb exudate and moisture of an affected part, has a good moisturizing effect, and can have a good treatment effect on burn infection, but does not solve the problems that the existing spraying film agent cannot form a breathable film when in use, is easy to dissolve in water, is easy to cause irritation to skin, is easy to cause film falling and the like, and therefore the composite antibacterial spraying film agent containing lysostaphin and chitosan is provided.
Disclosure of Invention
The invention aims to provide a composite antibacterial film spraying agent of lysostaphin and chitosan, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the raw materials for preparing the composite gel comprise, by mass volume, 1-20% of lysostaphin, 0.1-5% of chitosan, 0.5-10% of a surface tension regulator, 0.5-5% of a pH regulator and the balance of medical purified water.
Preferably, the chitosan molecular weight is 10000-500000 and the degree of deacetylation is > 80%.
Preferably, the surface tension regulator comprises one or more of short chain alcohol, polysiloxane and derivatives thereof, polyoxyethylene and derivatives thereof, and fluorine surfactant.
Preferably, the pH regulator includes lactic acid, glacial acetic acid, hydrochloric acid, and a buffer salt.
5. The compound antibacterial film spraying agent of lysostaphin and chitosan according to claim 1, which is characterized in that: the preparation method comprises the following preparation steps:
s1, adjusting the pH value of the medical pure water under aseptic conditions: mixing medical purified water with a proper amount of pH regulator, and slowly mixing and stirring the medical purified water when regulating the pH value of the purified water;
s2, adding chitosan into the mixed solution S1 to form a solution: fully swelling chitosan, stirring and uniformly mixing to obtain a chitosan solution, adjusting the pH value to 3.0-6.0, then carrying out ultrasonic defoaming and filtering;
s3, adding lysostaphin to the solution in S2: adding lysostaphin and surface tension regulator into the filtrate obtained in S2, stirring, mixing, quantitatively loading the obtained film-spraying solution into pressure-resistant container, and spraying the solution in the pressure-resistant container onto affected part.
Preferably, in the step S1, the stirring motor is used for stirring, and the rotation speed of the stirring motor is 100-500 r/min.
Preferably, the pH value in S1 is adjusted within the range of 0.5-4.0.
Preferably, the rotation speed of the stirring motor in S2 is 1500-.
Preferably, the ultrasonic frequency of the ultrasonic wave defoaming in S2 is 20000-40000Hz, and the filter screen for filtering is a silver wire filter screen.
Preferably, the rotation speed of the stirring motor for stirring and mixing in S3 is 1000-.
Compared with the prior art, the invention has the beneficial effects that:
after the composite antibacterial film spraying agent disclosed by the invention is sprayed on a skin wound, a film can be formed quickly, the film forming maintenance time is long, bleeding and blood coagulation can be quickly realized, complicated steps of replacing gauze and the like and damaging the wound can be avoided, the original bacterial infection wound surface is isolated by a water-insoluble protective film formed on the wound surface, the isolation and sterilization effects are realized, and meanwhile, the film can prevent external bacterial secondary infection while ensuring certain air permeability of the wound.
The composite antibacterial film spraying agent disclosed by the invention has extremely strong killing effect on gram-positive bacteria and gram-negative bacteria, is wide in antibacterial range, has no stimulation to skin, and is safe and convenient to use.
The surface tension regulator is added into the composite antibacterial film spraying agent disclosed by the invention, so that a water-insoluble protective film is formed on a wound surface by chitosan, the film is not easy to remove, the film maintenance time is long, and the medicine can effectively enter an affected part to generate a biochemical reaction to generate a better medicine effect.
Drawings
FIG. 1 is a schematic view of the step structure of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution:
the first embodiment is as follows:
the raw materials for preparing the composite gel comprise, by mass volume, 1% of lysostaphin, 0.5% of chitosan, 0.1% of a surface tension regulator, 0.6% of a pH regulator and the balance of medical purified water.
In order to make the molecules fine enough to make the formed film form a breathable film, the composite antibacterial film spraying agent can effectively kill gram-positive and gram-negative bacteria, promote wound healing and tissue repair, and has no irritation to skin, in this embodiment, preferably, the chitosan has a molecular weight of 10000 and a deacetylation degree of 80%.
In order to enable the surface of the film to form tension, not to cause the film to fall off, or not to cause the skin to be pulled and injured again, the composite antibacterial film spraying agent can effectively and rapidly form a water-insoluble protective film on the wound surface, the film shape is well maintained for a long time, the film is not easy to deform, the wound surface can be effectively and strongly isolated, the wound healing is promoted, and the infection is prevented, in the embodiment, preferably, the surface tension regulator comprises one or more of short-chain alcohol, polysiloxane and derivatives thereof, polyoxyethylene and derivatives thereof, and fluorine surfactant.
In order to adjust the PH and prevent the PH from irritating the skin, in this embodiment, the surface of the film formed by the composite antibacterial film spraying agent is smooth and uniform in thickness, and has good air permeability, and preferably, the PH adjusting agent includes lactic acid, glacial acetic acid, hydrochloric acid, and buffer salt.
In order to realize the preparation of the composite antibacterial film spraying agent, in this embodiment, the following preparation steps are preferably included:
s1, adjusting the pH value of the medical pure water under aseptic conditions: mixing medical purified water with a proper amount of pH regulator, and slowly mixing and stirring the medical purified water when regulating the pH value of the purified water;
s2, adding chitosan into the mixed solution S1 to form a solution: fully swelling chitosan, stirring and uniformly mixing to obtain a chitosan solution, adjusting the pH to 4.0, then carrying out ultrasonic defoaming and filtering;
s3, adding lysostaphin to the solution in S2: adding lysostaphin and surface tension regulator into the filtrate obtained in S2, stirring, mixing, quantitatively loading the obtained film-spraying solution into pressure-resistant container, and spraying the solution in the pressure-resistant container onto affected part.
In order to prevent the solution from being sprayed when the PH is adjusted, in this embodiment, it is preferable that the slow mixing and stirring in S1 is performed by using a stirring motor, and the rotation speed of the stirring motor is 100 r/min.
In order to make the PH of the solution moderate and to buffer the subsequent solution addition, in this embodiment, it is preferable that the PH adjustment range in S1 is 5.0.
In order to increase the mixing speed of the solution and prevent the substances in the solution from concentrating and being unable to be effectively mixed, in this embodiment, the rotation speed of the stirring motor in S2 is preferably 1500 r/min.
In order to filter the foam and the impurities in the solution and achieve the disinfection and sterilization effects, in this embodiment, it is preferable that the ultrasonic frequency of the ultrasonic defoaming in S2 is 20000Hz, and a silver wire filter screen is adopted as the filter screen for filtering.
In order to achieve the mixing of the solution without causing precipitation due to too high speed, in this embodiment, it is preferable that the rotation speed of the stirring motor for stirring and mixing in S3 is 1000 r/min.
Example two:
the raw materials for preparing the composite gel comprise, by mass volume, 5% of lysostaphin, 2% of chitosan, 2% of a surface tension regulator, 0.9% of a pH regulator and the balance of medical purified water.
In order to make the molecules fine enough to form a breathable film, the composite antibacterial film spraying agent can effectively kill gram-positive and gram-negative bacteria, promote wound healing and tissue repair, and has no irritation to skin, in this embodiment, preferably, the molecular weight of the chitosan is 500000, and the deacetylation degree is 85%.
In order to enable the surface of the film to form tension, not to cause the film to fall off, or not to cause the skin to be pulled and injured again, the composite antibacterial film spraying agent can effectively and rapidly form a water-insoluble protective film on the wound surface, the film shape is well maintained for a long time, the film is not easy to deform, the wound surface can be effectively and strongly isolated, the wound healing is promoted, and the infection is prevented, in the embodiment, preferably, the surface tension regulator comprises one or more of short-chain alcohol, polysiloxane and derivatives thereof, polyoxyethylene and derivatives thereof, and fluorine surfactant.
In order to adjust the PH and prevent the PH from irritating the skin, in this embodiment, the surface of the film formed by the composite antibacterial film spraying agent is smooth and uniform in thickness, and has good air permeability, and preferably, the PH adjusting agent includes lactic acid, glacial acetic acid, hydrochloric acid, and buffer salt.
In order to realize the preparation of the composite antibacterial film spraying agent, in this embodiment, the following preparation steps are preferably included:
s1, adjusting the pH value of the medical pure water under aseptic conditions: mixing medical purified water with a proper amount of pH regulator, and slowly mixing and stirring the medical purified water when regulating the pH value of the purified water;
s2, adding chitosan into the mixed solution S1 to form a solution: fully swelling chitosan, stirring and uniformly mixing to obtain a chitosan solution, adjusting the pH value to 3.0, then carrying out ultrasonic defoaming and filtering;
s3, adding lysostaphin to the solution in S2: adding lysostaphin and surface tension regulator into the filtrate obtained in S2, stirring, mixing, quantitatively loading the obtained film-spraying solution into pressure-resistant container, and spraying the solution in the pressure-resistant container onto affected part.
In order to prevent the solution from being sprayed when the PH is adjusted, in this embodiment, it is preferable that the slow mixing and stirring in S1 is performed by using a stirring motor, and the rotation speed of the stirring motor is 500 r/min.
In order to make the PH of the solution moderate and to buffer the subsequent solution addition, in this embodiment, it is preferable that the PH adjustment range in S1 is 4.0.
In order to increase the mixing speed of the solution and prevent the substances in the solution from concentrating and being unable to be effectively mixed, in this embodiment, the rotation speed of the stirring motor in S2 is preferably 2000 r/min.
In order to filter the foam and the impurities in the solution and achieve the disinfection and sterilization effects, in this embodiment, it is preferable that the ultrasonic frequency of the ultrasonic defoaming in S2 is 40000Hz, and the filter screen for filtering is a silver wire filter screen.
In order to achieve the mixing of the solution without causing precipitation due to too high speed, in this embodiment, it is preferable that the rotation speed of the stirring motor for stirring and mixing in S3 is 1500 r/min.
Example three:
preparing 1% (mass volume percentage) hydrochloric acid solution by using medical purified water and hydrochloric acid under the aseptic condition, adding 3% (mass volume percentage) chitosan with molecular weight of 200000 and deacetylation degree of 90%, stirring to fully swell to obtain chitosan solution, adjusting pH to 3.0-6.0, filtering after ultrasonic defoaming, adding 10% (mass volume percentage) lysostaphin and 4% (mass volume percentage) surface tension regulator into the filtrate, stirring and mixing uniformly, quantitatively filling the prepared spray film solution into a pressure-resistant container, and uniformly spraying the solution in the pressure-resistant container on an affected part when in use.
Example four:
preparing hydrochloric acid solution with the concentration of 3% (mass volume percentage) by using medical purified water and hydrochloric acid under the aseptic condition, adding 5% (mass volume percentage) of chitosan with the molecular weight of 500000 and the deacetylation degree of 95%, stirring to fully swell the chitosan solution to obtain chitosan solution, adjusting the pH value to 3.0-6.0, filtering after ultrasonic defoaming, adding 20% (mass volume percentage) of lysostaphin and 5% (mass volume percentage) of surface tension regulator into the filtrate, stirring and mixing uniformly, quantitatively filling the prepared spray film solution into a pressure-resistant container, and uniformly spraying the solution in the pressure-resistant container on an affected part when in use.
According to the quality detection in GB T14449-:
| item | Index (I) |
| PH value (25 ℃, stock solution) | 3.0-6.0 |
| Methanol content (mg/kg) | 0 |
| Arsenic content (mg/kg) | 0 |
| Heavy Metal content (mg/kg) | 0 |
According to the experiment, the preparation is a pure natural product, is non-toxic and harmless, cannot cause irritation to skin, and is convenient to use;
| item | Index improvement |
| Air tightness | The content is increased by 150 percent |
| Zhang Li | Increased by 100 percent |
| Adhesive ability | The improvement is 130 percent |
| Whether or not it is soluble in water | Is insoluble in water |
| Duration of maintenance | The improvement is 140 percent |
Through the experimental detection, the product can effectively realize the adhesion capability, so that the product can be adhered for a longer time without falling off, the air tightness of the film is better, anaerobic respiration of cells can be prevented, wound compounding is prevented, and the long-time adhesion can improve the pharmacology and increase the absorption of the drug effect.
The working principle and the using process of the invention are as follows:
firstly, adjusting the pH value of medical pure water under aseptic condition: mixing medical purified water with a proper amount of pH regulator, and slowly mixing and stirring the medical purified water when regulating the pH value of the purified water;
secondly, adding chitosan into the S1 mixed solution to form a solution: fully swelling chitosan, stirring and uniformly mixing to obtain a chitosan solution, adjusting the pH value to 3.0, then carrying out ultrasonic defoaming and filtering;
thirdly, adding lysostaphin to the solution in S2: adding lysostaphin and surface tension regulator into the filtrate obtained in S2, stirring, mixing, quantitatively loading the obtained film-spraying solution into pressure-resistant container, and spraying the solution in the pressure-resistant container onto affected part.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A composite antibacterial film spraying agent of lysostaphin and chitosan is characterized in that: the raw materials for preparing the composite gel comprise, by mass volume, 1-20% of lysostaphin, 0.1-5% of chitosan, 0.5-10% of a surface tension regulator, 0.5-5% of a pH regulator and the balance of medical purified water.
2. The compound antibacterial film spraying agent of lysostaphin and chitosan according to claim 1, which is characterized in that: the chitosan molecular weight is 10000-500000 and the degree of deacetylation is > 80%.
3. The compound antibacterial film spraying agent of lysostaphin and chitosan according to claim 1, which is characterized in that: the surface tension regulator comprises one or more of short-chain alcohol, polysiloxane and derivatives thereof, polyoxyethylene and derivatives thereof, and fluorine surfactant.
4. The compound antibacterial film spraying agent of lysostaphin and chitosan according to claim 1, which is characterized in that: the pH regulator comprises lactic acid, glacial acetic acid, hydrochloric acid and buffer salt.
5. The compound antibacterial film spraying agent of lysostaphin and chitosan according to claim 1, which is characterized in that: the preparation method comprises the following preparation steps:
s1, adjusting the pH value of the medical pure water under aseptic conditions: mixing medical purified water with a proper amount of pH regulator, and slowly mixing and stirring the medical purified water when regulating the pH value of the purified water;
s2, adding chitosan into the mixed solution S1 to form a solution: fully swelling chitosan, stirring and uniformly mixing to obtain a chitosan solution, adjusting the pH value to 3.0-6.0, then carrying out ultrasonic defoaming and filtering;
s3, adding lysostaphin to the solution in S2: adding lysostaphin and surface tension regulator into the filtrate obtained in S2, stirring, mixing, quantitatively loading the obtained film-spraying solution into pressure-resistant container, and spraying the solution in the pressure-resistant container onto affected part.
6. The composite antibacterial film spraying agent of lysostaphin and chitosan according to claim 5, which is characterized in that: in the step S1, the stirring motor is used for stirring, and the rotation speed of the stirring motor is 100-500 r/min.
7. The composite antibacterial film spraying agent of lysostaphin and chitosan according to claim 5, which is characterized in that: the pH value in the S1 is adjusted within the range of 0.5-4.0.
8. The composite antibacterial film spraying agent of lysostaphin and chitosan according to claim 5, which is characterized in that: the rotating speed of the stirring motor in the S2 is 1500-2000 r/min.
9. The composite antibacterial film spraying agent of lysostaphin and chitosan according to claim 5, which is characterized in that: the ultrasonic frequency range of the ultrasonic wave defoaming in the S2 is 20000-40000Hz, and the filtering screen adopts a silver wire filtering screen.
10. The composite antibacterial film spraying agent of lysostaphin and chitosan according to claim 5, which is characterized in that: the rotating speed rate of the stirring motor for stirring and mixing in the S3 is 1000-1500 r/min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113440645A (en) * | 2021-06-29 | 2021-09-28 | 西安汇朴成医疗科技有限公司 | Composite lysozyme liquid dressing for wound surface and preparation method thereof |
| CN114668006A (en) * | 2022-04-19 | 2022-06-28 | 陕西康禾立丰生物科技药业有限公司 | Biological disinfectant based on natural tartaric acid and preparation process thereof |
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| CN104856978A (en) * | 2015-05-18 | 2015-08-26 | 福建中医药大学 | Chitosan spraying film agent and preparation method thereof |
| CN107174573A (en) * | 2017-05-04 | 2017-09-19 | 艾伯尔生物科技（重庆）有限公司 | Wound surface protection film spray and preparation method thereof |
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| CN101721691A (en) * | 2008-11-04 | 2010-06-09 | 上海高科联合生物技术研发有限公司 | Preparation for treating and restoring infective wound surface and preparation method thereof |
| CN104856978A (en) * | 2015-05-18 | 2015-08-26 | 福建中医药大学 | Chitosan spraying film agent and preparation method thereof |
| CN107174573A (en) * | 2017-05-04 | 2017-09-19 | 艾伯尔生物科技（重庆）有限公司 | Wound surface protection film spray and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113440645A (en) * | 2021-06-29 | 2021-09-28 | 西安汇朴成医疗科技有限公司 | Composite lysozyme liquid dressing for wound surface and preparation method thereof |
| CN114668006A (en) * | 2022-04-19 | 2022-06-28 | 陕西康禾立丰生物科技药业有限公司 | Biological disinfectant based on natural tartaric acid and preparation process thereof |
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