CN112430662B - Kit for predicting lung squamous cell carcinoma prognosis risk and application thereof - Google Patents
Kit for predicting lung squamous cell carcinoma prognosis risk and application thereof Download PDFInfo
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Abstract
The invention discloses a kit for predicting lung squamous cell carcinoma prognosis risk and application thereof, and relates to the technical field of genetic engineering and oncology, the kit can detect sequence information of a target region, the target region is selected from regions 1-127 in table 1 and is a specific region located in ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR, OR7A5 and RUNX1 genes, and the kit can effectively and quickly predict the prognosis risk of a patient with lung squamous cell carcinoma tumor by detecting the sequence information of the target region, and compared with other clinical markers, the mutation of the target regions has higher specificity and is convenient and quick to detect.
Description
Technical Field
The invention relates to the technical field of genetic engineering and oncology, in particular to a kit for predicting lung squamous cell carcinoma prognosis risk and application thereof.
Background
Lung cancer is one of the most rapidly growing malignancies that threaten human health and life. Currently, surgical resection remains the first means of cancer therapy to improve patient survival. Especially for patients with squamous cell lung carcinoma. Squamous cell carcinoma of the lung, also known as squamous cell carcinoma of the lung, including spindle cell carcinoma, is the most common type, accounting for 40% -51% of primary lung cancers. Squamous cell lung cancer is commonly seen in old men and has close relation with smoking. Squamous cell lung cancer is common in central lung cancer, and tends to grow in the chest cavity, and early squamous cell lung cancer often causes bronchoconstriction or obstructive pulmonary inflammation. Squamous cell lung carcinoma has slow growth, late metastasis, more chance of surgical resection, and higher survival rate in 5 years, and is less sensitive to radiotherapy and chemotherapy than small cell undifferentiated carcinoma.
Even locally advanced squamous cell lung carcinoma patients should undergo surgical resection and select adjuvant radiotherapy or chemotherapy according to their pathological characteristics, as long as they are compatible with the surgical indications (e.g., surgically resectable stage iiia). The key is whether or not to select the postoperative adjuvant therapy and how to select the postoperative adjuvant therapy. Whether the adjuvant radiotherapy and chemotherapy should be received after the operation is mainly based on the cancer stage according to the consensus of the existing experts (2018 version of the expert of thoracic surgery in the adjuvant therapy of non-small cell lung cancer after the operation, which is hereinafter referred to as consensus). However, consensus does not give high confidence recommendations for patients in certain cases (e.g., stage IIa patients with tumors >4cm, ≦ 5cm, stage IIIa patients). In clinical practice, it is usually necessary to combine other factors to evaluate the prognosis of the operation and to determine the treatment regimen of the postoperative chemotherapy. Therefore, finding an accurate, rapid, simple and convenient post-operative prognostic marker is an urgent clinical need for determining post-operative chemoradiotherapy protocols.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a kit for predicting lung squamous cell carcinoma prognosis risk and application thereof.
The invention is realized by the following steps:
in a first aspect, the embodiments of the present invention provide a use of an agent for detecting a target region for preparing a kit for predicting a risk of prognosis of squamous cell lung cancer, wherein the target region comprises: a part or all of at least 1 region among the regions 1 to 127 shown in Table 1;
TABLE 1 target regions
In a second aspect, embodiments provide a kit for predicting the prognostic risk of squamous cell lung cancer, comprising reagents for detecting a target region comprising part or all of at least 1 region of regions 1-127 as set forth in table 1.
In a third aspect, the embodiment provides a method for predicting the lung squamous carcinoma prognosis risk, which comprises the steps of predicting the lung squamous carcinoma prognosis risk based on the obtained sequence information of the corresponding target region in the genome of a sample to be tested; the target area comprises part or all of at least 1 area in areas 1-127 shown in Table 1; the methods are not directed towards the treatment or diagnosis of disease.
In a fourth aspect, embodiments provide an apparatus for predicting a prognostic risk of squamous cell lung cancer, comprising:
the prediction module is used for predicting the prognosis risk of the sample to be detected according to the sequence information of the corresponding target region in the genome of the sample to be detected;
wherein the target region comprises part or all of at least 1 region in the regions 1-127 shown in Table 1.
In a fifth aspect, an embodiment provides an electronic device, including: a memory, a processor and a computer program stored on the memory and executable on the processor, the processor when executing the computer program implementing the method for predicting a prognostic risk of squamous cell lung cancer as described in the previous embodiments.
In a sixth aspect, embodiments provide a computer readable medium storing a computer program executable by a processor to implement the method for predicting a risk of prognosis of squamous cell lung cancer as described in the previous embodiments.
The invention has the following beneficial effects:
the kit can effectively and quickly predict the prognosis risk of patients with squamous cell lung carcinoma by detecting the mutation condition of a target region, wherein the target region is selected from regions 1-127 in table 1 and is a specific region located in ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR, OR7A5 and RUNX1 genes, and the prognosis risk of patients with squamous cell lung carcinoma can be effectively and quickly predicted by detecting the sequence information of the target region, and the mutation of the target region has higher specificity and is convenient and quick to detect compared with other clinical markers.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a graph of survival plots for 188 patients carrying and not carrying mutations in the target region in example 3 of the present invention;
FIG. 2 is a ROC graph of the prediction result of KNN method in example 3 of the present invention;
FIG. 3 is a ROC plot for test group 1 in example 4 of the present invention;
FIG. 4 is a ROC plot for test group 2 in example 4 of the present invention;
FIG. 5 is a ROC plot for test group 3 in example 4 of the present invention;
FIG. 6 is a ROC plot for test group 4 in example 4 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Firstly, the embodiment of the invention provides an application of a reagent for detecting a target region in preparing a kit for predicting lung squamous carcinoma prognosis risk, wherein the target region comprises part or all of at least 1 region in regions 1-127 shown in table 1;
the detection target region being a reagent means sequence information of the detection target region. The regions 1 to 20 belong to the specific target region of the ARID1A gene, the region 21 to the specific target region of the SLC43A2 gene, the regions 22 to 45 to the specific target region of the ANKS1A gene, the regions 46 to 83 to the specific target region of the CHD2 gene, the regions 84 to 117 to the specific target region of the RPTOR gene, the region 118 to the specific target region of the OR7A5 gene, and the regions 119 to 127 to the specific target region of the RUNX1 gene. In addition, the information of the target region is in the version of the human genome hg 19.
The "prognostic risk" as described above may refer to the recovery, or survival, of a patient after surgery. The population with long survival time is the population with low risk, the population with short survival time is the population with low risk, and the length of the survival time is relatively speaking, and the survival time of the population with high risk is shorter than that of the population with low risk. A preset time limit can be set, the people with low risk are judged if the life time is longer than the preset time limit, and the people with high risk are judged if the life time is shorter than the preset time limit. The preset period can be set to any value in 1-80 months, and specifically can be 36, 48 or 60 months.
Through a series of creative efforts, the inventors find that the mutation conditions of specific regions of ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR, OR7A5 and RUNX1 genes have high correlation with the postoperative risk of squamous cell lung cancer patients, and the mutation of the specific regions has higher specificity (more than 90 percent) relative to other clinical markers, is convenient and quick to detect, and can effectively and quickly predict the postoperative prognosis of the squamous cell lung cancer patients.
Preferably, the target region comprises at least 44 of regions 1-127; preferably, the target region includes at least 82 regions of regions 1-127. Preferably, the target region includes at least 117 of regions 1-127. Preferably, the target region includes at least 118 of regions 1-127.
Preferably, the reagent includes any one of a probe, a primer and a chip. The nucleic acid sequences used for the primers, probes and chips are not particularly limited, and any reagents capable of detecting the target region can be used for preparing a kit for predicting the prognosis risk of squamous cell lung carcinoma, and the kit is within the protection scope of the present application. Preferably, the probes comprise at least 1 probe in the probes 1-288; the sequences of the probes 1-288 are respectively shown in SEQ ID Nos. 1-288.
Secondly, the embodiment of the invention provides a kit for predicting the prognosis risk of squamous cell lung cancer, which comprises a reagent for detecting a target region, wherein the target region comprises at least 1 region in the regions 1-127, and the information of the regions 1-127 refers to the table 1. Preferably, the target region comprises at least 44 of regions 1-127; preferably, the target region comprises at least 82 regions of regions 1-127; preferably, the target region includes at least 117 of regions 1-127. Preferably, the target region comprises regions 1-127.
Through a series of researches, the inventor finds that the detection of the sequences of specific regions of ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR, OR7A5 and RUNX1 genes by using the kit can effectively evaluate the postoperative prognosis risk of patients based on the detection condition (whether mutation occurs).
Preferably, the reagent includes any one of a probe, a primer and a chip; preferably, the probes comprise at least 1 probe in the probes 1-288; the sequences of the probes 1-288 are respectively shown in SEQ ID Nos. 1-288.
The embodiment of the invention also provides a method for predicting the lung squamous cell carcinoma prognosis risk, which comprises the steps of predicting the lung squamous cell carcinoma prognosis risk based on the obtained sequence information of the corresponding target region in the genome of a sample to be tested; the methods are not directed towards the treatment or diagnosis of disease.
The target area comprises at least 1 area in the areas 1-127, and the information of the areas 1-127 refers to the table 1.
The method is not targeted to living human or animal bodies and is not directed towards obtaining disease diagnosis or health status. But only a method of detecting a sample of tissue, body fluid or excreta that has been detached from a human or animal body to obtain information as a risk of prognosis. It should be noted that the detection method can be operated only by a third-party detection company, and the detection result can be used for auxiliary diagnosis or auxiliary treatment, but the diagnosis result of the disease or the health condition of the patient cannot be directly obtained according to the result content of the prognosis risk. The detection of the target region may be by any common high throughput sequencing method, such as amplicon sequencing (PCR-based amplification sequencing), or probe and region hybridization sequencing (Hybrid capture-based target amplification sequencing). The length of the region with a coverage of more than 30X in the target region must account for 100% of the total detection region length, and the sequencing results must pass the quality control criteria of the sequencing service provider.
Preferably, the target region comprises at least 44 of regions 1-127; preferably, the target area comprises at least 82 areas of the areas 1-127, preferably the target area comprises the areas 1-127. Preferably, the method comprises the steps of detecting by using at least 1 probe in the probes 1-288; the base sequences of the probes 1-288 are shown as SEQ ID Nos. 1-288 in sequence.
Preferably, the predicting the prognosis risk of squamous cell lung carcinoma comprises: and based on the sequence information, judging the sample to be detected with mutation in the target region as a high-risk sample, otherwise, judging the sample to be detected as a low-risk sample.
The embodiment of the invention also provides a device for predicting the lung squamous carcinoma prognosis risk, which comprises:
the prediction module is used for predicting the prognosis risk of the sample to be detected according to whether the sequence information of the corresponding target region in the genome of the sample to be detected is mutated; the target area comprises at least 1 area in the areas 1-127, and the information of the areas 1-127 refers to the table 1. Preferably, the target region comprises at least 44 of regions 1-127; preferably, the target region comprises at least 82 regions of regions 1-127; preferably, the target region comprises at least 117 of regions 1-127; preferably, the target region comprises regions 1-127.
Preferably, the prediction module further comprises: and based on the sequence information, marking the sample to be detected with mutation in the target region as a high-risk sample, and otherwise, marking the sample to be detected as a low-risk sample. Preferably, the apparatus further comprises: and the acquisition module is used for acquiring the sequence information of the corresponding target region in the genome of the sample to be detected.
An embodiment of the present invention further provides an electronic device, which includes: a memory, a processor and a computer program stored on the memory and executable on the processor, the processor implementing the method for predicting risk of prognosis of squamous cell lung cancer as described in any of the previous embodiments when the computer program is executed by the processor.
It should be noted that the electronic device may include a memory, a processor, a bus, and a communication interface, which are electrically connected directly or indirectly to each other to realize the transmission or interaction of data. For example, the components may be electrically connected to each other via one or more buses or signal lines. The processor may process information and/or data related to target identification to perform one or more of the functions described herein.
The Memory may be, but is not limited to, a Read Only Memory (ROM), an Erasable Programmable Read-Only Memory (EPROM), a Programmable Read-Only Memory (PROM), a Random Access Memory (RAM), an electrically Erasable Programmable Read-Only Memory (EEPROM), and the like. The processor may be an integrated circuit chip having signal processing capabilities. The Processor may be a general-purpose Processor, including a Network Processor (NP), a Central Processing Unit (CPU), and the like; but also Digital Signal Processors (DSPs), Field-Programmable Gate arrays (FPGAs), Application Specific Integrated Circuits (ASICs) or other Programmable logic devices, discrete Gate or transistor logic devices, discrete hardware components. The components in the electronic device may be implemented in hardware, software, or a combination thereof. In practical applications, the electronic device may be a server, a tablet computer, a notebook computer, a cloud platform, a mobile phone, an ultra-mobile personal computer (UMPC), a Personal Digital Assistant (PDA), a netbook, a handheld computer, a wearable electronic device, a virtual reality device, and the like, and therefore, the type of the electronic device is not limited in the embodiments of the present application.
Embodiments of the present invention also provide a computer readable medium storing a computer program, which can be executed by a processor to implement the method for predicting risk of lung squamous carcinoma prognosis as described in any of the foregoing embodiments.
The computer readable storage medium may be mutually equivalent to the memory in the foregoing embodiments.
Example 1
The present embodiments provide a kit for predicting the prognostic risk of squamous cell lung cancer, comprising reagents for detecting a target region. The target area includes areas 1-127, and the specific information of the target area is described with reference to table 1. The reagent comprises probes 1-288, the sequences of the probes 1-288 are shown in SEQ ID No. 1-288 in sequence, and refer to Table 2 specifically.
TABLE 2 Probe
Example 2
The present example provides a method for predicting the prognostic risk of squamous cell lung cancer, which comprises detecting the prognostic risk of squamous cell lung cancer in a nucleic acid sample from a patient with squamous cell lung cancer using the reagent provided in example 1.
In this embodiment, the probes 1 to 288 are used to detect the regions 1 to 127. It is noted that in other embodiments, detection of the target region may be performed using existing high throughput sequencing methods. The following steps were performed after sequencing: (a) sequencing data quality was checked using Fastqc; (b) removing reads with average sequencing quality less than 28 and reads with length less than 74bp (read length) by using TRIMMATIC; (c) mapping the sequencing result to a human genomic reference sequence using bwa, or equivalent software; (d) obtaining a processed BAM file by using a Data pre-processing flow in the GATK Best Practices workflow; (e) somatic variations (including point mutations and short indels) in tumor samples were detected using GATK mutect 2; (f) and filtering out the mutation with the mutant reads support number less than 3 by using GATK Filter MutectCalls to obtain a VCF file for mutation detection.
And judging the sample to be detected with mutation in the target region as a high-risk sample based on the sequence information of the target region obtained by sequencing, and otherwise, judging the sample to be detected as a low-risk sample. The method specifically comprises the following steps: and f, the VCF file in the step f is annotated by the oncotor software to obtain a maf result file. An entry with any one of the Hugo _ Symbol column values ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR, OR7A5, RUNX1 is selected in this file. An entry having the variable _ Classification column "noise _ detail" is removed. If the remaining entries are present within the range of the target regions listed in Table 1, then this sample is judged to be a high risk sample, otherwise the sample is a low risk sample.
Example 3
In this example, 188 patients with squamous cell lung carcinoma of 41-86 years of age were collected, wherein 71 patients in stage I, 65 patients in stage II, 50 patients in stage III, and 2 patients in stage IV were determined to have tumor tissues carrying sequence information of specific regions (regions 1-127) of ARID1A, SLC43a2, ANKS1A, CHD2, RPTOR, OR7a5, and RUNX1 genes in the method provided in example 2, and the relationship between the patients and the patients after determining the sequence information of the target region and the survival period after surgery was analyzed, and the analysis results are shown in table 3.
TABLE 3P and HR values of the genes in different survival assays
| Gene | One-way Log rank analysis | Multifactor Cox analysis |
| ARID1A | 6.08x10-5 | 1.40x10-5(4.03) |
| SLC43A2 | 6.05x10-4 | 1.93x10-5(4.12) |
| ANKS1A | 3.34x10-2 | 7.64x10-3(3.89) |
| CHD2 | 1.08x10-3 | 1.30x10-3(2.89) |
| RPTOR | 2.98x10-3 | 1.66x10-3(2.99) |
| OR7A5 | 4.41x10-5 | 6.92x10-6(160.00) |
| RUNX1 | 1.79x10-3 | 3.67x10-3(5.06) |
Remarking: the HR values of the gene in the multifactorial Cox analysis are shown in parentheses.
The P and HR values (Hazard ratio, same below) for the seven genes in the single and multifactorial survival assays are shown in Table 3, each of which is highly correlated with patient survival (P values less than 0.01) and is a poor prognostic factor (HR value greater than 1). The average HR was 26.14, i.e. the high risk population had a 26.14 times higher risk of death than the low risk population.
Furthermore, the postoperative life span of the patient is more than or equal to 57 months, which is defined as good prognosis; conversely, a prognosis is poor < 57 months. Patients carrying at least one of the regions 1-127 with a mutation to a mutant form, otherwise called wild-type without a mutation, were tested using the Fisher exact test hypothesis. See fig. 1 and table 4 for test results.
TABLE 4 Fisher exact test hypothesis test results
| Mutant forms | Wild type | Total of | |
| Good prognosis | 74(63.8％) | 68(94.4％) | 142 |
| Poor prognosis | 42(36.2％) | 4(5.6％) | 46 |
| Total of | 116(100％) | 72(100％) | 188 |
From the results, it was found that mutations in specific regions (target regions) of seven genes were significantly negatively correlated with prognosis (table 4). Further, 57 months was defined as the threshold values for good prognosis (low risk) and poor prognosis (high risk), and the learning curve of the results was predicted using the KNN (K-Nearest Neighbors) method, with reference to fig. 2. In fig. 2, the horizontal axis represents the false positive rate and the vertical axis represents the true positive rate, and it can be seen from the results that the sensitivity of the method for predicting the prognosis risk of squamous cell lung cancer provided in example 2 was 0.520 when the area under the ROC curve was 0.712 and the specificity was 0.909.
Example 4
In this example, 4 test groups were set, and 188 patients with squamous cell lung carcinoma of 41-86 years of age (same as example 3) were tested according to the method of example 2. The detection difference of the 4 test groups is the difference of the detection areas, and the table 5 is specifically referred.
TABLE 5 test results
Remarking: the detection regions of test group 1 were all regions (regions 1 to 118) corresponding to ARID1A, SLC43A2, ANKS1A, CHD2, RPTOR and OR7A5 genes in Table 1; by analogy, the detection areas of the test group 2 are areas 1-117, the detection areas of the test group 3 are areas 46-127, and the detection areas of the test group 4 are areas 84-127.
The results are shown in Table 5 and FIGS. 3 to 6.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
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<400> 18
agcagccagc accctcgacg ctctcccagc aggctgcgta tcctcagccc cagtctcagc 60
agtcccagca aactgcctat tcccagcagc gcttccctcc accgcaggta agatatccct 120
<210> 19
<211> 120
<212> DNA
<213> Artificial sequence
<400> 19
aggagctatc tcaagattca tttgggtctc aggcatcctc agccccctca atgacctcca 60
gtaagggagg gcaagaagat atgaacctga gccttcagtc aagaccctcc agcttgcctg 120
<210> 20
<211> 120
<212> DNA
<213> Artificial sequence
<400> 20
ttgaaagtag aatctttctg cctaatatta ctaatccatg ttcttatata tatgttctag 60
gatctatctg gttcaataga tgacctcccc atggggacag aaggagctct gagtcctgga 120
<210> 21
<211> 120
<212> DNA
<213> Artificial sequence
<400> 21
gtgagcacat cagggatttc cagcagccaa ggagagcaga gtaatccagc tcagtctcct 60
ttctctcctc atacctcccc tcacctgcct ggcatccgag gcccttcccc gtcccctgtt 120
<210> 22
<211> 120
<212> DNA
<213> Artificial sequence
<400> 22
ggctctcccg ccagtgttgc tcagtctcgc tcaggaccac tctcgcctgc tgcagtgcca 60
ggtaccctca agtgctgggc tttagggaga gggaaaggtg actgccccca gtaatattaa 120
<210> 23
<211> 120
<212> DNA
<213> Artificial sequence
<400> 23
ctttgtctgg agcaggcaac cagatgccac ctcggccacc cagtggccag tcggacagca 60
tcatgcatcc ttccatgaac caatcaagca ttgcccaaga tcgaggtgag agcctgggtg 120
<210> 24
<211> 120
<212> DNA
<213> Artificial sequence
<400> 24
taaagctaat aactatatgg atgctaccca caaataggtt atatgcagag gaacccccag 60
atgccccagt acagttcccc ccagcccggc tcagccttat ctccgcgtca gccttccgga 120
<210> 25
<211> 120
<212> DNA
<213> Artificial sequence
<400> 25
ggacagatac acacaggcat gggctcctac cagcagaact ccatggggag ctatggtccc 60
caggggggtc agtatggccc acaaggtcag tatactaccc agttaggagt agatacgggt 120
<210> 26
<211> 120
<212> DNA
<213> Artificial sequence
<400> 26
agtcttgtct tcctcccctc ccaggtggct accccaggca gccaaactat aatgccttgc 60
ccaatgccaa ctaccccagt gcaggcatgg ctggaggcat aaaccccatg ggtgccggag 120
<210> 27
<211> 120
<212> DNA
<213> Artificial sequence
<400> 27
gtcaaatgca tggacagcct ggcatcccac cttatggcac actccctcca gggaggatga 60
gtcacgcctc catgggcaac cggccttatg gccctaacat ggccaatatg ccacctcagg 120
<210> 28
<211> 120
<212> DNA
<213> Artificial sequence
<400> 28
ttgggtcagg gatgtgtccc ccaccagggg gcatgaaccg gaaaacccaa gaaactgctg 60
tcgccatgca tgttgctgcc aactctatcc aaaacaggta aggcctggga agcagagagg 120
<210> 29
<211> 120
<212> DNA
<213> Artificial sequence
<400> 29
ctcagagtct aacctttgtc tctctcactt tccatcttct tccttaggcc gccaggctac 60
cccaatatga atcaaggggg catgatggga actggacctc cttatggaca agggattaat 120
<210> 30
<211> 120
<212> DNA
<213> Artificial sequence
<400> 30
agtatggctg gcatgatcaa ccctcaggga cccccatatt ccatgggtgg aaccatggcc 60
aacaattctg caggtaagtg ctagtcattc tcactaggga tttcttcaag agtcacatca 120
<210> 31
<211> 120
<212> DNA
<213> Artificial sequence
<400> 31
tacagggatg gcagccagcc cagagatgat gggccttggg gatgtaaagt taactccagc 60
caccaaaatg aacaacaagg cagatgggac acccaagaca gaatccaaat ccaaggtagt 120
<210> 32
<211> 120
<212> DNA
<213> Artificial sequence
<400> 32
acctgaacct tccagaaatc cagttcttct actacaacca atgagaagat caccaagttg 60
tatgagctgg gtggtgagcc tgagaggaag atgtgggtgg accgttatct ggccttcact 120
<210> 33
<211> 120
<212> DNA
<213> Artificial sequence
<400> 33
gaggagaagg ccatgggcat gacaaatctg cctgctgtgg gtaggaaacc tctggacctc 60
tatcgcctct atgtgtctgt gaaggagatt ggtggattga ctcaggtgag tgggcgcctg 120
<210> 34
<211> 120
<212> DNA
<213> Artificial sequence
<400> 34
ttggtttggt tatacaggtc aacaagaaca aaaaatggcg ggaacttgca accaacctca 60
atgtgggcac atcaagcagt gctgccagct ccttgaaaaa gcagtatatc cagtgtctct 120
<210> 35
<211> 120
<212> DNA
<213> Artificial sequence
<400> 35
atgcctttga atgcaagatt gaacggggag aagaccctcc cccagacatc tttgcagctg 60
ctgattccaa gaagtcccag cccaagatcc agcctccctc tcctggtaag gatggggtca 120
<210> 36
<211> 120
<212> DNA
<213> Artificial sequence
<400> 36
gagttaaaca ctgtcatgcc aagcaaacta ctcaacttgt atctctgtcc acagcgggat 60
caggatctat gcaggggccc cagactcccc agtcaaccag cagttccatg gcagaaggag 120
<210> 37
<211> 120
<212> DNA
<213> Artificial sequence
<400> 37
gagacttaaa gccaccaact ccagcatcca caccacacag tcagatcccc ccattgccag 60
gcatgaggta aggccaagag caggggcaga tggttgggag gatggctgaa gataagtgca 120
<210> 38
<211> 120
<212> DNA
<213> Artificial sequence
<400> 38
tttgtggttt acttggtttt cctcactctg gagcaggagc aattcagttg ggatccagga 60
tgcctttaat gatggaagtg actccacatt ccagaagcgg aattccatga ctccaaaccc 120
<210> 39
<211> 120
<212> DNA
<213> Artificial sequence
<400> 39
tgggtatcag cccagtatga atacctctga catgatgggg cgcatgtcct atgagccaaa 60
taaggatcct tatggcagca tgaggaaagg tgactgatct gattgctatt tgaacttgtg 120
<210> 40
<211> 120
<212> DNA
<213> Artificial sequence
<400> 40
ttgtcaactt accagtttgt tcaccgcttg cctttctacg ctcagctcca gggagtgatc 60
ccttcatgtc ctcagggcag ggccccaacg gcgggatggg tgacccctac agtcgtgctg 120
<210> 41
<211> 120
<212> DNA
<213> Artificial sequence
<400> 41
ccggccctgg gctaggaaat gtggcgatgg gaccacgaca gcactatccc tatggaggtc 60
cttatgacag agtgaggtaa gcatgacccc agctcctgtc cactccccca gcaccctgaa 120
<210> 42
<211> 120
<212> DNA
<213> Artificial sequence
<400> 42
cagcaccctg aagctatagt gggctcaatc tgcctctcca attttgttta ggacggagcc 60
tggaataggg cctgagggaa acatgagcac tggggcccca cagccgaatc tcatgccttc 120
<210> 43
<211> 120
<212> DNA
<213> Artificial sequence
<400> 43
caacccagac tcggggatgt attctcctag ccgctacccc ccgcagcagc agcagcagca 60
gcagcaacgg tgagtaaagc ctggtctcgg tgctgctatg gatcaggctt cgccactgcc 120
<210> 44
<211> 120
<212> DNA
<213> Artificial sequence
<400> 44
tgcctcctat agacatgatt cctatggcaa tcagttctcc acccaaggca ccccttctgg 60
cagccccttc cccagccagc agactacaat gtatcaacag caacagcagg tgaggagggt 120
<210> 45
<211> 120
<212> DNA
<213> Artificial sequence
<400> 45
tgtgtccacc aagcatctgg ttgtagccat cttggcatct gtgggcttta tgtccctgag 60
tgcagagtat taacttcccc tctgcttgtc tctgccttag aattacaagc ggccaatgga 120
<210> 46
<211> 119
<212> DNA
<213> Artificial sequence
<400> 46
tggcacatat ggccctcctg ccaagcggca cgaaggggag atgtacagcg tgccatacag 60
cactgggcag gggcagcctc agcagcagca gttgccccca gcccagcccc agcctgcca 119
<210> 47
<211> 120
<212> DNA
<213> Artificial sequence
<400> 47
cagcaacaag ctgcccagcc ttcccctcag caagatgtat acaaccagta tggcaatgcc 60
tatcctgcca ctgccacagc tgctactgag cgccgaccag caggcggccc ccagaaccaa 120
<210> 48
<211> 120
<212> DNA
<213> Artificial sequence
<400> 48
tttccattcc agtttggccg agaccgtgtc tctgcacccc ctggcaccaa tgcccagcaa 60
aacatgccac cacaaatgat gggcggcccc atacaggcat cagctgaggt tgctcagcaa 120
<210> 49
<211> 120
<212> DNA
<213> Artificial sequence
<400> 49
ggcaccatgt ggcaggggcg taatgacatg acctataatt atgccaacag gcagagcacg 60
ggctctgccc cccagggccc cgcctatcat ggcgtgaacc gaacagatga aatgctgcac 120
<210> 50
<211> 120
<212> DNA
<213> Artificial sequence
<400> 50
acagatcaga gggccaacca cgaaggctcg tggccttccc atggcacacg ccagccccca 60
tatggtccct ctgcccctgt gccccccatg acaaggcccc ctccatctaa ctaccagccc 120
<210> 51
<211> 120
<212> DNA
<213> Artificial sequence
<400> 51
ccaccaagca tgcagaatca cattcctcag gtatccagcc ctgctcccct gccccggcca 60
atggagaacc gcacctctcc tagcaagtct ccattcctgc actctgggat gaaaatgcag 120
<210> 52
<211> 120
<212> DNA
<213> Artificial sequence
<400> 52
aaggcaggtc ccccagtacc tgcctcgcac atagcacctg cccctgtgca gccccccatg 60
attcggcggg atatcacctt cccacctggc tctgttgaag ccacacagcc tgtgttgaag 120
<210> 53
<211> 120
<212> DNA
<213> Artificial sequence
<400> 53
cagaggaggc ggctcacaat gaaagacatt ggtaaggaga tcttcctcat tcggttgcct 60
aatctgcccc tttccatctt gtccattgtt ccctccacct tactattctg gatgaggttg 120
<210> 54
<211> 120
<212> DNA
<213> Artificial sequence
<400> 54
ggtgcctcca gccaacctgg gcttggtgga tagacgacat ggaggtttat ttcaggaacc 60
ccggaggcat ggcgggtaat gatgtccctc aagtctggtc tcctggcaga gagcacatgg 120
<210> 55
<211> 120
<212> DNA
<213> Artificial sequence
<400> 55
gcattagata ccatcaacat cctgctgtat gatgacaaca gcatcatgac cttcaacctc 60
agtcaggtga gtatcagtgc ctggggaaga ttgagagggt ttgggatctt cttagtgtag 120
<210> 56
<211> 120
<212> DNA
<213> Artificial sequence
<400> 56
tttcctgttc tttctctttt tagctcccag ggttgctaga gctccttgta gaatatttcc 60
gacgatgcct gattgagatc tttggcattt taaaggagta tgaggtgggt gacccaggac 120
<210> 57
<211> 119
<212> DNA
<213> Artificial sequence
<400> 57
agagaacgct actggatcct gggaggttca gcaaggtgtc tagtccagct cccatggagg 60
gtggggaaga agaagaagaa cttctaggtc ctaaactaga agaggaagaa gaagaggaa 119
<210> 58
<211> 120
<212> DNA
<213> Artificial sequence
<400> 58
gtagttgaaa atgatgagga gatagccttt tcaggcaagg acaagccagc ttcagagaat 60
agtgaggaga agctgatcag taagtttgac aagcttccag taaagatcgt acagaagaat 120
<210> 59
<211> 120
<212> DNA
<213> Artificial sequence
<400> 59
gatccatttg tggtggactg ctcagataag cttgggcgtg tgcaggagtt tgacagtggc 60
ctgctgcact ggcggattgg tgggggggac accactgagc atatccagac ccacttcgag 120
<210> 60
<211> 120
<212> DNA
<213> Artificial sequence
<400> 60
agcaagacag agctgctgcc ttcccggcct cacgcaccct gcccaccagc ccctcggaag 60
catgtgacaa cagcagaggg tacaccaggg acaacagacc aggaggggcc cccacctgat 120
<210> 61
<211> 120
<212> DNA
<213> Artificial sequence
<400> 61
ggacctccag aaaaacggat cacagccact atggatgaca tgttgtctac tcggtctagc 60
accttgaccg aggatggagc taagagttca gaggccatca aggagagcag caagtttcca 120
<210> 62
<211> 120
<212> DNA
<213> Artificial sequence
<400> 62
tttggcatta gcccagcaca gagccaccgg aacatcaaga tcctagagga cgaaccccac 60
agtaaggatg agaccccact gtgtaccctt ctggactggc aggattctct tgccaagcgc 120
<210> 63
<211> 120
<212> DNA
<213> Artificial sequence
<400> 63
tgcgtctgtg tgtccaatac cattcgaagc ctgtcatttg tgccaggcaa tgactttgag 60
atgtccaaac acccagggct gctgctcatc ctgggcaagc tgatcctgct gcaccacaag 120
<210> 64
<211> 120
<212> DNA
<213> Artificial sequence
<400> 64
cacccagaac ggaagcaggc accactaact tatgaaaagg aggaggaaca ggaccaaggg 60
gtgagctgca acaaagtgga gtggtggtgg gactgcttgg agatgctccg ggaaaacacc 120
<210> 65
<211> 120
<212> DNA
<213> Artificial sequence
<400> 65
ttggttacac tcgccaacat ctcggggcag ttggacctat ctccataccc cgagagcatt 60
tgcctgcctg tcctggacgg actcctacac tgggcagttt gcccttcagc tgaagcccag 120
<210> 66
<211> 120
<212> DNA
<213> Artificial sequence
<400> 66
gacccctttt ccaccctggg ccccaatgcc gtcctttccc cgcagagact ggtcttggaa 60
accctcagca aactcagcat ccaggacaac aatgtggacc tgattctggc cacacccccc 120
<210> 67
<211> 120
<212> DNA
<213> Artificial sequence
<400> 67
ttcagccgcc tggagaagtt gtatagcact atggtgcgct tcctcagtga ccgaaagaac 60
ccggtgtgcc gggagatggc tgtggtactg ctggccaacc tggctcaggg ggacagcctg 120
<210> 68
<211> 120
<212> DNA
<213> Artificial sequence
<400> 68
gcagctcgtg ccattgcagt gcagaagggc agtatcggca acctcctggg cttcctagag 60
gacagccttg ccgccacaca gttccagcag agccaggcca gcctcctcca catgcagaac 120
<210> 69
<211> 120
<212> DNA
<213> Artificial sequence
<400> 69
ccaccctttg agccaactag tgtggacatg atgcggcggg ctgcccgcgc gctgcttgcc 60
ttggccaagg tggacgagaa ccactcagag tttactctgt acgaatcacg gctgttggac 120
<210> 70
<211> 120
<212> DNA
<213> Artificial sequence
<400> 70
atctcggtat caccgttgat gaactcattg gtttcacaag tcatttgtga tgtactgttt 60
ttgattggcc agtcatgaca gccgtgggac acctcccccc cccgtgtgtg tgtgcgtgtg 120
<210> 71
<211> 120
<212> DNA
<213> Artificial sequence
<400> 71
cctccccctt aatatttaag aattaaaaga tgatgagaaa taaggacaaa agccaagagg 60
aggacagttc gctacacagc aatgcatcga ggtatgagct atcaactttc ttcctttttg 120
<210> 72
<211> 120
<212> DNA
<213> Artificial sequence
<400> 72
ataagtcact cagcctctga agaagcttcg ggttcagact caggcagtca gtcggaaagt 60
gagcagggaa gtgatccagg aagtggacat ggcagcgagt cgaacagcag ctctgaatct 120
<210> 73
<211> 120
<212> DNA
<213> Artificial sequence
<400> 73
tctgagagtc agtcggaatc tgagagcgaa tcagcaggtt ccaaatccca gccagtcctc 60
ccagaagcca aagagaagcc agcctctaag aaggaacgga tagctgatgt gaagaaggta 120
<210> 74
<211> 120
<212> DNA
<213> Artificial sequence
<400> 74
gtctcttaat tttacagatg tgggaagaat atcctgatgt ttatggggtc aggcggtcaa 60
accgaagcag acaagaacca tcgcgattta atattaagga agaggtaagg aaaaaatgtt 120
<210> 75
<211> 120
<212> DNA
<213> Artificial sequence
<400> 75
aatcattttt cccccctcac acactgaagg caagtagcgg gtctgagagt gggagcccaa 60
aaagaagagg ccagaggcag ctgaaaaaac agtaagtctt tcatggggga aattattcaa 120
<210> 76
<211> 120
<212> DNA
<213> Artificial sequence
<400> 76
atcacagaga aaaatggaaa caggaaccct cagaagatga acaggaacaa ggcaccagtg 60
cagagagtga gccagaacaa aaaaaagtaa aagccagaag acctgtcccc agaaggtgca 120
<210> 77
<211> 120
<212> DNA
<213> Artificial sequence
<400> 77
accaaatgat aaagtgaaga cttagccttt tgtttctctt ctcattttag aacagtgccc 60
aaacctcgtg ttaaaaagca gccgaagact cagcgtggaa agagaaaaaa gcaagattct 120
<210> 78
<211> 120
<212> DNA
<213> Artificial sequence
<400> 78
tctgatgagg atgatgatga tgacgaagct cccaaaaggc agactcgtcg aagagcggct 60
aaaaacgtta ggtaagttgt accccagaag tgtttcactg gtgtgatgtg ataaagtagt 120
<210> 79
<211> 120
<212> DNA
<213> Artificial sequence
<400> 79
gtttaattct gaagagatca tttctcattt attcagcatt attcctcttg cagttacaaa 60
gaagatgatg actttgagac tgactcagat gatctcattg aaatgactgg agaaggagtt 120
<210> 80
<211> 120
<212> DNA
<213> Artificial sequence
<400> 80
gatgaacagc aagataatag tgaaactatt gaaaaggtct tagattcaag actgggaaag 60
aaaggaggta tgtgtatttg gggagcaaat tacattttat gtatgcttag taagacttag 120
<210> 81
<211> 120
<212> DNA
<213> Artificial sequence
<400> 81
tttgcagcca ctggagcatc tactactgta tatgcgattg aagctaatgg cgaccctagt 60
ggtgactttg acactgaaaa ggatgaaggt gaaatccagt acctcatcaa gtggaagggt 120
<210> 82
<211> 120
<212> DNA
<213> Artificial sequence
<400> 82
tggtcttaca tccacagcac atgggagagt gaagaatcct tacagcaaca gaaagtgaag 60
ggcctaaaaa aactagagaa cttcaagaaa aaagaggacg aaatcaaaca atggtatatt 120
<210> 83
<211> 120
<212> DNA
<213> Artificial sequence
<400> 83
tgccattcag gttagggaaa gtttctcctg aagatgtaga atatttcaat tgccaacagg 60
agctggcttc agagttgaat aaacagtatc agatagtaga aagagtaata ggtaagtaca 120
<210> 84
<211> 120
<212> DNA
<213> Artificial sequence
<400> 84
tgtttataac ttttctgtct tattttttat ttgtttagct gtgaagacaa gtaaatctac 60
attgggtcaa acagattttc caggtaagca agaaatttta tttataaatg ttcttcaaca 120
<210> 85
<211> 120
<212> DNA
<213> Artificial sequence
<400> 85
ctaatctata aatttttttt atatgaaata gctcatagtc ggaagccggc accctcaaat 60
gagcccgaat atctatgtaa atggatggga ctcccctatt cagagtgtag ctgggaagat 120
<210> 86
<211> 120
<212> DNA
<213> Artificial sequence
<400> 86
gaagccctca ttggaaagaa attccagaat tgcattgaca gcttccacag taggaacaac 60
tcaaaaacca tcccaacaag agaatgcaag gtatggtgat ggttggcttt tgttttttca 120
<210> 87
<211> 120
<212> DNA
<213> Artificial sequence
<400> 87
gttttaacat agtagcagta agaacatttt ctcagacttg ggcttttgtt tttcaggccc 60
tgaagcagag accacgattt gtagctttaa agaaacaacc tgcatattta ggaggggaga 120
<210> 88
<211> 120
<212> DNA
<213> Artificial sequence
<400> 88
atctggaact tcgagattat cagctagaag gtctaaactg gctagctcat tcctggtgca 60
agtaggtaga aaaatatgag tgcaattttc cttactgttt ctggcttctt tattgttaga 120
<210> 89
<211> 120
<212> DNA
<213> Artificial sequence
<400> 89
ttctttctgc agaaataata gtgtaatcct tgctgatgaa atgggcctag gaaagaccat 60
ccagaccata tcattcctct cctacctgtt ccaccaacac cagctgtatg gcccctttct 120
<210> 90
<211> 120
<212> DNA
<213> Artificial sequence
<400> 90
tatagtcgtc cctttatcca ccctcacctc atggcagaga gagtttgaaa tctgggcacc 60
agagattaac gtagtggttt acataggtga cctgatgagc agaaatacgg tgtgtaaaca 120
<210> 91
<211> 120
<212> DNA
<213> Artificial sequence
<400> 91
gtttttttct tatagatacg ggaatatgaa tggattcatt cccaaaccaa aagattgaag 60
ttcaacgcac ttataacaac atatgagatc ctcttgaaag ataaggtgtg taattaatat 120
<210> 92
<211> 120
<212> DNA
<213> Artificial sequence
<400> 92
tcgttctgtt ttgtttttac tttagactgt gctgggcagt attaactggg cctttctggg 60
agtggatgaa gcccatcggt tgaagaatga tgactcttta ttgtataaaa ctctgattga 120
<210> 93
<211> 120
<212> DNA
<213> Artificial sequence
<400> 93
tttcaagtcc aaccataggc tcctgattac ggggacccct cttcagaatt ccctcaaaga 60
gctctggtcc ttgctgcact ttattatgcc ggagaagtaa gctccttcct gtgtatttca 120
<210> 94
<211> 120
<212> DNA
<213> Artificial sequence
<400> 94
ataacactat actgtttctt ccctaggttt gaattttggg aagattttga agaagaccat 60
gggaagggga gagaaaatgg ctaccagagt cttcataagg tgctagagcc tttccttctc 120
<210> 95
<211> 120
<212> DNA
<213> Artificial sequence
<400> 95
cggagagtca aaaaagatgt ggagaaatcc cttcctgcta aagtggaaca gattctcagg 60
gtggagatgt cagcccttca gaaacagtat tacaagtaag ttcttgtttg ggttaggcct 120
<210> 96
<211> 120
<212> DNA
<213> Artificial sequence
<400> 96
ttttgtatct agtagtatca ttatcatttt aatttgcagg tggattctga ccaggaatta 60
caaggctctt gccaaaggaa caagaggcag cacatctggt tttcttaata ttgtgatgga 120
<210> 97
<211> 120
<212> DNA
<213> Artificial sequence
<400> 97
actgaaaaaa tgttgcaacc actgctatct gattaaaccc cctgaagaaa atgaaaggga 60
aaatggacag gagattcttc tggtaggtag ttcctcataa ttactttctc aaaaaaaacg 120
<210> 98
<211> 120
<212> DNA
<213> Artificial sequence
<400> 98
tttgtgtttc aacataatta ttggaatgtc ttttaaatct tcagtccctc ataaggagca 60
gtgggaagtt gattttatta gacaaactgt tgacaagact tcgagaaagg gggaatcgag 120
<210> 99
<211> 120
<212> DNA
<213> Artificial sequence
<400> 99
tgcttatctt ctctcagatg gtgagaatgt tggatatcct ggctgaatac ctaactatta 60
aacactatcc tttccaggta aggtgatttc agtaattgct gtggggggaa tcaatctctc 120
<210> 100
<211> 120
<212> DNA
<213> Artificial sequence
<400> 100
gttttcttgt ggtttatttt tacagcgtct ggatggttcc atcaagggag aaatccgaaa 60
acaggcactg gaccacttca atgcagatgg gtctgaggta tactatgcat ggctttgtta 120
<210> 101
<211> 120
<212> DNA
<213> Artificial sequence
<400> 101
tctgagaagg ccactgcata aagtagtata ttgcattgtg tacaggactt ctgtttcctg 60
ctctcgacaa gggctggtgg cctgggaatc aatttggctt cagcggacac agtcgtcatc 120
<210> 102
<211> 120
<212> DNA
<213> Artificial sequence
<400> 102
tttgactctg actggaaccc ccagaatgac ttgcaggcac aagcccgagc gcatagaatt 60
ggtcaaaaga agcaggtcag tatggagagg cttctggaaa ttgctttagg gttgggggcc 120
<210> 103
<211> 120
<212> DNA
<213> Artificial sequence
<400> 103
ttggggggtg gttcaggcat aagcataaca gttccttttc ctacaggtaa atatttaccg 60
cttagttaca aaggggactg tggaggagga gatcatagaa cgggccaaaa agaagatggt 120
<210> 104
<211> 120
<212> DNA
<213> Artificial sequence
<400> 104
attagatcat ctggtgattc agcgcatgga caccactggc cggacgatcc tggaaaacaa 60
ctcaggaagg tccaagtaag tgccaggaag attgggaggt aggcagaatc aaattgattc 120
<210> 105
<211> 120
<212> DNA
<213> Artificial sequence
<400> 105
ttacttccac agctcaaatc cttttaataa agaagagctg acagctattt tgaaatttgg 60
agcagaggat ctcttcaaag aactggaagg ggaggaatca gaacctcagg taattaacaa 120
<210> 106
<211> 120
<212> DNA
<213> Artificial sequence
<400> 106
ttttgttctt ttaggaaatg gatatagatg aaattttgcg gttggctgaa acgagagaga 60
atgaagtgtc aacaagtgca acagatgaac ttctatcaca gtttaaggta tgaagatctt 120
<210> 107
<211> 120
<212> DNA
<213> Artificial sequence
<400> 107
agggaaatgt cagacaatgg gttgtctgtt ttaaggttgc caactttgca acaatggaag 60
atgaagaaga gctagaagag cgtcctcaca aggactggga tgagatcatt ccagaggaac 120
<210> 108
<211> 120
<212> DNA
<213> Artificial sequence
<400> 108
aaaggaaaaa agtagaggag gaagagcggc agaaggagct agaagaaatt tatatgctgc 60
ctcgaattcg gagttccact aaaaaggtga tcaagtgaga tgaaagatat gaaattattt 120
<210> 109
<211> 120
<212> DNA
<213> Artificial sequence
<400> 109
gttacagtgt gactttgcct cgatctttct caggctcaga caaatgacag tgactctgac 60
actgagtcta agaggcaggc ccagagatcc tctgcttctg agagtgaaac ggaagactct 120
<210> 110
<211> 120
<212> DNA
<213> Artificial sequence
<400> 110
gatgatgaca agaagccaaa gcgcagaggg cgtccgagga gtgtgcggaa ggacctcgtg 60
gagggattta ctgatgcaga gatccgaagg ttggtggagg ctctgttctc actgagcttg 120
<210> 111
<211> 120
<212> DNA
<213> Artificial sequence
<400> 111
ctctgttttt ttaatatgtt ttattgatcc tattcttagg ttcatcaagg cttataagaa 60
gtttggtctc cctcttgaac ggtaagttca gtgtaatagt cccttatctt cctctttttt 120
<210> 112
<211> 120
<212> DNA
<213> Artificial sequence
<400> 112
agaagagtgg gcacagggtc ctaaagtgtc cccatatttg ttcctcctca ggctggagtg 60
catagcacgt gatgctgagc tggtagataa gtcggtggca gatctgaagc gcctgggtga 120
<210> 113
<211> 120
<212> DNA
<213> Artificial sequence
<400> 113
actgatccac aacagctgtg tgtcagcaat gcaggaatac gaagagcagc tgaaagaaaa 60
tgccagcgag ggtaagcgaa gttggcttta gtgagtcttc gcaacctggc actcttggac 120
<210> 114
<211> 120
<212> DNA
<213> Artificial sequence
<400> 114
tggaacttct gcagattttc atttaactaa tgtgaaactg gtatttttca ggaaaaggac 60
cagggaaaag gagaggtcca acaatcaaga tatccggagt tcaggttaat gtgaaatcca 120
<210> 115
<211> 120
<212> DNA
<213> Artificial sequence
<400> 115
ttatccaaca tgaagaggag tttgagatgc tgcataaatc tatccctgtg gaccctgaag 60
aaaaaaaaaa gtgagtatat tttgtgtaca tgcttagatg gtcgtaccgt aagaaaatag 120
<210> 116
<211> 120
<212> DNA
<213> Artificial sequence
<400> 116
aaaggcccct cttctaatgt cttcctgttt ttaaactttc tttagatact gcttaacctg 60
tcgtgtcaaa gctgcacatt ttgatgtaga gtggggggtg gaagatgatt ctcgcctgtt 120
<210> 117
<211> 120
<212> DNA
<213> Artificial sequence
<400> 117
gctggggatt tatgaacatg gctatggaaa ctgggagtta attaaaacag acccagagct 60
taaattaact gacaaagtaa gtaaccctac catgctagag atttctagaa gatttgttgt 120
<210> 118
<211> 120
<212> DNA
<213> Artificial sequence
<400> 118
ctgatccact aaccaggatg tgggtggtgg cgggtgcttc tcttcctttc ttgttgaaga 60
ttctgccggt ggagacagat aaaaagcctc aggggaagca gctacagacc cgagcggatt 120
<210> 119
<211> 120
<212> DNA
<213> Artificial sequence
<400> 119
acttgttgaa gctgctcaga aagggtctgg agaagaaggg ggctgtgaca ggtggggaag 60
aggtgagtac gctgccagct ggttgttttt caggggcctg aggctcctac cctgcagaat 120
<210> 120
<211> 120
<212> DNA
<213> Artificial sequence
<400> 120
tatgcttttg agtgtcttga tttgtatttt aatcatcatt ttctctcctt ttccaggcca 60
aattaaagaa gcggaagcct cgggtaaaga aggaaaacaa agtgcccagg ctgaaagagg 120
<210> 121
<211> 120
<212> DNA
<213> Artificial sequence
<400> 121
agcatggaat tgagctttca tctcctaggc attcagataa tccatcagaa gagggagaag 60
tgaaagtatg aagtggggtt tcggttgagg gttatttatt tattttagct atacttatca 120
<210> 122
<211> 119
<212> DNA
<213> Artificial sequence
<400> 122
ggggcagtgc ttctttttcc agaaagtaca taagtagata taaaatttgt agcaaaaatt 60
catagccctg ttttgtttcc taggatgatg gcttggaaaa aagtccaatg aaaaaaaaa 119
<210> 123
<211> 120
<212> DNA
<213> Artificial sequence
<400> 123
tcaaaagata agaaagagaa ggtaatgatg cccttctgtt catgcagata tccacagcct 60
ttgcaattcg ccatttggat ttagaagtag aatgagattt gagggccctg gaataattgt 120
<210> 124
<211> 120
<212> DNA
<213> Artificial sequence
<400> 124
gcacggtagg attgcacaaa tgacaatgac tccttgtaac tcttttttaa aagcctaaaa 60
gtggtgatgc caaatcttcg agtaaatcaa agcgatctca gggtcctgtc catattacag 120
<210> 125
<211> 120
<212> DNA
<213> Artificial sequence
<400> 125
caggaagtga acctgtcccc attggagagg atgaggatga tgatctggac caggagacat 60
tcagcatagt aagtcttgaa atcggggggt gccagtgtct gcagccgcgg tacttgctgt 120
<210> 126
<211> 120
<212> DNA
<213> Artificial sequence
<400> 126
tttatgtctt tactctgttg tcctgttgca gtgtaaggag aggatgaggc ccgtgaaaaa 60
ggcactgaaa cagctcgaca aacctgacaa ggggctcaac gtgcaagaac agctggaaca 120
<210> 127
<211> 120
<212> DNA
<213> Artificial sequence
<400> 127
cacccggaac tgcctgctga aaatcggaga ccggatagcc gagtgcctta aagcctactc 60
agatcaggag cacatcaaac tctggaggag gtaaccactt tggcctcgtc tgcccagttt 120
<210> 128
<211> 120
<212> DNA
<213> Artificial sequence
<400> 128
tttcttttta ggaacctatg gatttttgtt tccaagttta cagaatttga tgctcgaaaa 60
ctgcataagt tatacaagat ggctcataag aaaaggtctc aagaagaaga ggtaaagtac 120
<210> 129
<211> 120
<212> DNA
<213> Artificial sequence
<400> 129
ttttactctt taggagcaaa agaagaaaga cgacgtgact gggggtaaga aaccatttcg 60
tccagaggcc tcaggctcca gccgggactc tctgatatct cagtcccata cctcacacaa 120
<210> 130
<211> 120
<212> DNA
<213> Artificial sequence
<400> 130
ccttcaccct cagaagcctc atttgcctgc ctcccatggc ccacagatgc atggacaccc 60
aagagataac tacaatcacc ccaacaagag acacttcagt aatgcaggta ggtcattaag 120
<210> 131
<211> 120
<212> DNA
<213> Artificial sequence
<400> 131
gaaagtgaaa ttcatccatt tcttgcagtc atcagatcat tctttctttt cctgcagatc 60
gaggagactg gcagagggaa agaaagttca actatggtgg tggcaacaac aatccaccat 120
<210> 132
<211> 120
<212> DNA
<213> Artificial sequence
<400> 132
ggggaagcga caggcaccat cagtatgagc agcactggta caaggaccac cattatgggg 60
accggcgaca tatggatgcc caccgttccg gaagctatcg acccaacaac atgtccagaa 120
<210> 133
<211> 120
<212> DNA
<213> Artificial sequence
<400> 133
agaggcctta tgaccagtac agcagtgacc gagaccaccg gggacacaga gattattatg 60
acaggtatgc aaaaggctgt gagacaccag gtgccaacct ttgccaggag ctgtttctag 120
<210> 134
<211> 120
<212> DNA
<213> Artificial sequence
<400> 134
atctctattt caggcaccat catgactcca agcggaggag atccgatgaa tttaggcctc 60
aaaattacca ccagcaggat ttccgacgaa tgtctgatca ccgccccgct atgggctacc 120
<210> 135
<211> 120
<212> DNA
<213> Artificial sequence
<400> 135
atggccaggg accctcagac cattaccgct ctttccacac agataaactg ggggaatata 60
aacagcctct acccccattg caccctgcag tctcagatcc tcgctcaccc ccttctcaga 120
<210> 136
<211> 120
<212> DNA
<213> Artificial sequence
<400> 136
aatctcctca cgattccaag tcacccctgg atcataggtc tcctttggag agatcactag 60
aacagaaaaa caacccagat tataactgga atgttcggaa aacataaagg acagctcgta 120
<210> 137
<211> 120
<212> DNA
<213> Artificial sequence
<400> 137
gccatccacc agcggcgccg atgggcagtg gccagggtgg gcgccatggt gcggcgcggc 60
gcggctccgg ctccggctcc ggctctgcac cacctgcgca cagaactcgg cgtcagcggc 120
<210> 138
<211> 120
<212> DNA
<213> Artificial sequence
<400> 138
cggcccttgc ccagcccctg cggccgcccc cagcagcccc gaccgctgcc gcagggctcg 60
ggattgccac ccctccccgg ccgcggcggc cggtgcgcgc agcagaggaa gcgaacccca 120
<210> 139
<211> 120
<212> DNA
<213> Artificial sequence
<400> 139
gccctgcccg gacctgggac accccgagcc cgcggccggc tcgtaccgct gccctccgct 60
acgacagagc cgccccggcc aggcagggag acgtctcccg cagctcactc cgaggtaaac 120
<210> 140
<211> 120
<212> DNA
<213> Artificial sequence
<400> 140
tgaggcatgg aggcaagaag gggctaacgc atgctcaggg ccgtggaact agtcctggcc 60
tgcagcagga ctgacatcgc ctggggccag catggaacgc agcgtgaggt gggcaggggc 120
<210> 141
<211> 120
<212> DNA
<213> Artificial sequence
<400> 141
agatccgagg gctgcctctc ccagggaccc tgacaggtgc cctcccgccc gcgcccgaga 60
cccttcccca gagctggttt cttcaaccac aagatcagcg cccagaccta cggaacggcc 120
<210> 142
<211> 120
<212> DNA
<213> Artificial sequence
<400> 142
cgccccggcg cgctcattgg ccggacggcg caggaactgc cgcgccattg gccgccgtct 60
aggtcgctag gcgctgcggc cacgcgaacc cagcgtggga gacgaggagc cggttcaaac 120
<210> 143
<211> 120
<212> DNA
<213> Artificial sequence
<400> 143
cggtggcgcg agccagccgc gcgaccccca tctcgggcgt tcccccggca actcgcccgt 60
ttccatggcc tcggaccccc gcgcctccat tccccagccc gcatctgttc gtcatctctt 120
<210> 144
<211> 120
<212> DNA
<213> Artificial sequence
<400> 144
ccctggcggc ccctcatcgc tccatctctc tcctcgttcc cacgttttct ccctacgccc 60
cttcggcggc cgcttctgcc cccgcgccct cgccccggcc gcgatcccct ccctgctgca 120
<210> 145
<211> 120
<212> DNA
<213> Artificial sequence
<400> 145
gctcacccgg ctcctccggc gtgtgtcggg cccgcccggc gccttttctg ccctctttta 60
ttccagccct tttattccga ccccaagaag aaaacactgc cgcccgctga ttggctgagc 120
<210> 146
<211> 120
<212> DNA
<213> Artificial sequence
<400> 146
ggggctggaa acaggcgtcg accaatcagc agccagcccc ccgcccgccc cgccgcgagc 60
agaagccggt tccggccgga ctcggagaca aacaagagag atgggggacg ggaggggcct 120
<210> 147
<211> 120
<212> DNA
<213> Artificial sequence
<400> 147
gggcgctgcg ggccgggcct cggctttgca gccttcaacc cgagtcacag cgcggccggg 60
aatccccggc gacatcgccc cgcggcctct ccagcggcct gaccctgcga ggctggggct 120
<210> 148
<211> 120
<212> DNA
<213> Artificial sequence
<400> 148
ccggctccgc gtcccggccc ccagcggact ccccccagaa gccgccgggc caccagcgcc 60
cccagccggg ctctcccgcg gccgccccgc cgtccccagc tgccccctgc gcgcgggtca 120
<210> 149
<211> 120
<212> DNA
<213> Artificial sequence
<400> 149
ccacgcccag atgcgccggc tccgcacggt ggttccactc tcgaatcccc accccgcctc 60
ctccccacgc agcgccgccg tccccgccgc ctcgggcttt gggtcctggt tcctcgctcc 120
<210> 150
<211> 120
<212> DNA
<213> Artificial sequence
<400> 150
cccgccccgg tggttcccgt cccggcgtat tcggggcctg ggatcccgga cccggtccag 60
ccgtggcgcc ccccgctgat gcaccgaccc cttcccctct ccccggtggc gtctgcccgg 120
<210> 151
<211> 120
<212> DNA
<213> Artificial sequence
<400> 151
tccctggaag ttggcccttg gaaatctgca aggggatttg gggctggtct gtcccgtcct 60
aggatggggt ctcctgagct ccccccatca gactgacctt tctccgcccg tgttcacgca 120
<210> 152
<211> 120
<212> DNA
<213> Artificial sequence
<400> 152
ctgctcggtc ctccggggac cctccaccct tgatcgtcat ctgggtgctc cctgcccgcg 60
ctggccacct tcaccccgtt ccccgtccct ttcagggaag gtcccctctg cagggcgatg 120
<210> 153
<211> 120
<212> DNA
<213> Artificial sequence
<400> 153
ctacgggcca cgtccgccag ctggtacaag ctagggaggg ttcgaggggg ctccgggtcc 60
ccgtctgata agcccgggct ccgaaagctc agctccccgt cttcgtcccc cggattcgag 120
<210> 154
<211> 119
<212> DNA
<213> Artificial sequence
<400> 154
gcactaaccc gacccgggaa gaccctctga ctgccccaga atcgccttgg tcaccctggc 60
tcgctccggg gggctggctt agcaccccag ggctgccgcg cggtgtccac gtctggaat 119
<210> 155
<211> 120
<212> DNA
<213> Artificial sequence
<400> 155
catctctacc aaaataaaaa ccaaaaataa aaccccacca ccaccccagg gctgtgggag 60
ccccaaacgc ctcttgctcc acctgcccca gtccgagtgt gccccctact gtctagaatc 120
<210> 156
<211> 120
<212> DNA
<213> Artificial sequence
<400> 156
ctgtctagaa tccgcccggg ccacctggcg agggcaacct cccccagaca acgcttggag 60
ctgtcagcct gctggggggc ggctccagct ggtagcctac ccggttcctc accctgtgga 120
<210> 157
<211> 120
<212> DNA
<213> Artificial sequence
<400> 157
tcaccctgtg gaacccttcc cactcccaag gtttcaactg ccatctgaaa aatctctttc 60
ttcacccttc cctcctcttt gagcagcctg acggcgctgc agggctcaaa catgcctcaa 120
<210> 158
<211> 120
<212> DNA
<213> Artificial sequence
<400> 158
aacatgcctc aaatatcccc cagaactgtc ctccacattt tctctcagat aaccatacta 60
ctactgtcta ctgaagttag ataccaagaa ccctgtcttt tttctttttc tttttttttt 120
<210> 159
<211> 120
<212> DNA
<213> Artificial sequence
<400> 159
catgactcac agtgccctgc tgagaaccat cctttcatca gacatttgtt aggcagtcgc 60
tgtgattctg cctctccctc accgcatccc catcaaactg atacagctgt atctcctaca 120
<210> 160
<211> 120
<212> DNA
<213> Artificial sequence
<400> 160
tccctcaccg catccccatc aaactgatac agctgtatct cctacatatt tacctaatcg 60
gtccatctgt gcccttaggc ccgcaactac tgttgttgag ccaccttcag atttcagttc 120
<210> 161
<211> 120
<212> DNA
<213> Artificial sequence
<400> 161
tctcaaaaaa ataaacaaaa tttaaaagtt ccagttcccc aatttgaccc acacaggctc 60
ctccctctcc ctccctctga aggacatctg acctgcctgg tccccgatgc tccagtcaca 120
<210> 162
<211> 120
<212> DNA
<213> Artificial sequence
<400> 162
tgaaggacat ctgacctgcc tggtccccga tgctccagtc acatggaatt gcctgtggtt 60
tctgcacctg ctgttctctc tcctctagct cgcgacatag cctcattttg gacggcacct 120
<210> 163
<211> 120
<212> DNA
<213> Artificial sequence
<400> 163
cgcttgctgc caaggactcc cccaccccct cccccactga tggagtccga aatgctgcaa 60
tcgcctcttc tgggcctggg ggaggaagat gaggctgatc ttacagactg gaacctacct 120
<210> 164
<211> 120
<212> DNA
<213> Artificial sequence
<400> 164
ttggctttta tgaaaaagag gcactgtgag aaaattgaag gctccaaatc cttagctcag 60
agctggagga tgaaggatcg ggtaagtgga ttctgaaggc accccttgaa cttggtagtt 120
<210> 165
<211> 120
<212> DNA
<213> Artificial sequence
<400> 165
tgttttcaga tgaagacagt cagtgttgcc ttagttttgt gcctgaatgt tggtgtggac 60
cctcccgatg tggtgaagac cacgccctgt gcacgcttgg aatgctggat cggtgagtat 120
<210> 166
<211> 120
<212> DNA
<213> Artificial sequence
<400> 166
ttttccattg cttcctcaga tcctctgtcg atgggtcctc agaaagctct ggaaaccatc 60
ggtgcaaatt tacagaagca gtacgagaac tggcagccaa gggtaagtgt gcttcacttg 120
<210> 167
<211> 120
<212> DNA
<213> Artificial sequence
<400> 167
ggagtccgtg gtaaatttct tcatttcttc tcctgcaaca ggcccggtac aagcagagcc 60
ttgacccaac tgtggatgaa gtcaagaagc tctgcacgtc cttacgtcgc aacgccaagg 120
<210> 168
<211> 120
<212> DNA
<213> Artificial sequence
<400> 168
aggagcgagt cctctttcac tacaatggcc acggggtgcc ccggcccaca gtcaacgggg 60
aggtctgggt cttcaacaag gtgggtgtgc cttccagctt ccttcccgtt tctgccaaaa 120
<210> 169
<211> 120
<212> DNA
<213> Artificial sequence
<400> 169
tattcctgag acgcacatgt aacgagcgca ctttgtgtgt tttctagaac tacacgcagt 60
acatccctct gtccatatat gacctgcaga cgtggatggg cagcccgtcg atcttcgtct 120
<210> 170
<211> 120
<212> DNA
<213> Artificial sequence
<400> 170
acgactgctc caatgctggc ttgatcgtca agtccttcaa gcagttcgca ctacagcggg 60
agcaggagct ggaggtgagc gctctggtgc ttggagagcg gtgctgggtt tggttttgtt 120
<210> 171
<211> 120
<212> DNA
<213> Artificial sequence
<400> 171
acactctctt ttcccgaatg ttctgccctt caggtagctg caatcaaccc aaatcaccct 60
cttgctcaga tgcctttgcc tccgtcgatg aaaaactgca tccagctggc agcctgcgag 120
<210> 172
<211> 120
<212> DNA
<213> Artificial sequence
<400> 172
gccaccgagc tgctgcccat gatccccgac ctcccggctg acctattcac ctcctgcctc 60
accaccccca tcaagatcgc cctgcgctgg tgagtggccc ctgctgtgcc cctgggaccc 120
<210> 173
<211> 120
<212> DNA
<213> Artificial sequence
<400> 173
atgccgttca cattcttttc tttctctgca ggttttgcat gcagaaatgt gtcagtctgg 60
tgcctggcgt cacactggat ttgatagaaa agtaagtagg atttttaagc tcttgtggct 120
<210> 174
<211> 120
<212> DNA
<213> Artificial sequence
<400> 174
tgttttctag gatccctggc cgcctgaacg acaggaggac gcccctgggt gaactgaact 60
ggatcttcac agccatcaca gacaccatcg cgtggaacgt gctcccccgg ggtgaggcgc 120
<210> 175
<211> 120
<212> DNA
<213> Artificial sequence
<400> 175
tagacacaag tcactgtaga ctccttttta tcttttatct gccctcagat ctcttccaaa 60
agctcttcag acaggacttg ctggtggcta gtctgtttcg aaatttttta ttggcggaaa 120
<210> 176
<211> 120
<212> DNA
<213> Artificial sequence
<400> 176
ggattatgag gtcgtataac tgcactcccg tcagcagccc gcgtctgccg cccacgtaca 60
tgcacgccat gtggtgagtg tttcaggatc ctccaggtgc cgtgctcccc cgccctccgt 120
<210> 177
<211> 120
<212> DNA
<213> Artificial sequence
<400> 177
gatttcctct gttctctccg caggcaagcc tgggacctgg ctgttgacat ctgtctgtct 60
cagctgccga cgatcatcga ggaaggcact gcgtttcggg tgagtccctc caagggcacc 120
<210> 178
<211> 120
<212> DNA
<213> Artificial sequence
<400> 178
gccccgcagc acagcccgtt cttcgccgag cagctgaccg cattccaggt gtggctcacc 60
atgggcgtgg agaaccgaaa cccacccgaa cagctgccca tcgtcctgca ggtgagtttc 120
<210> 179
<211> 120
<212> DNA
<213> Artificial sequence
<400> 179
tttcttcttg ttcttccagg tgctgttaag ccaagtgcac cggctgagag cattggactt 60
gcttggaaga tttttggacc tgggtccctg ggcagtgagc ctggtgcgtg ccttccgcat 120
<210> 180
<211> 120
<212> DNA
<213> Artificial sequence
<400> 180
cccaggcctt gtctgtcggc atcttcccct acgtgctgaa gctgctccag agctcggccc 60
gagagctgcg gccacttctc gttttcatct gggccaagat cctcgcagtg gacagcgtga 120
<210> 181
<211> 120
<212> DNA
<213> Artificial sequence
<400> 181
ctcctctgtc tctttctgtc cagtcgtgcc aagcggacct cgtgaaggac aacggccaca 60
agtacttcct gtcggtcctg gcggacccct acatgccagt aaggacgggg cagcacgctc 120
<210> 182
<211> 120
<212> DNA
<213> Artificial sequence
<400> 182
cgaccaggac tggtttttgt tttccaggct gaacaccgga ccatgacggc tttcattctc 60
gccgtgatcg tcaacagcta tcacacgggg caggtgagcc ccccagccac ccccagcccc 120
<210> 183
<211> 120
<212> DNA
<213> Artificial sequence
<400> 183
cctgtctgtg gtcccgcctc tgcaggaagc ctgccttcag ggaaacctca ttgccatctg 60
cctggagcag ctcaacgacc cgcacccctt gctgcgccag tgggtggcca tctgcctcgg 120
<210> 184
<211> 120
<212> DNA
<213> Artificial sequence
<400> 184
caggatctgg cagaacttcg actcggcgag gtggtgcggc gtgagggaca gcgctcatga 60
gaagctctac agcctcctct ccgaccccat tcccgaggtg agtcaggcgg gggctcagag 120
<210> 185
<211> 120
<212> DNA
<213> Artificial sequence
<400> 185
gcagcatggc ccggtgtggg acatgcctgt gaccccccgc cgccttgcag gtccgctgcg 60
cagcggtctt cgcccttggc acgttcgtgg gcaactctgc agagaggacg gaccactcca 120
<210> 186
<211> 120
<212> DNA
<213> Artificial sequence
<400> 186
ccaccatcga ccacaacgtg gccatgatgc tggcccagct ggtcagcgac gggagcccca 60
tggtccggaa ggtgcgtgaa cccccagccc ggcagcagca gggcacccag gctggacccc 120
<210> 187
<211> 120
<212> DNA
<213> Artificial sequence
<400> 187
ggagctggtg gtggctctga gtcatcttgt ggttcagtat gaaagcaatt tctgcaccgt 60
ggccctgcag ttcatagaag aggaaaagaa ctacgccttg ccttctccag caaccacagg 120
<210> 188
<211> 120
<212> DNA
<213> Artificial sequence
<400> 188
ggcctccacc tggaagccca ttgtaatttg tctttctcct tctttcccag agggagggag 60
tttgacccca gtgcgagaca gcccgtgcac ccccagactt cgttctgtga gctcctatgg 120
<210> 189
<211> 120
<212> DNA
<213> Artificial sequence
<400> 189
aaacatccgt gctgtcgcca cagccaggag cctcaacaaa tctttgcaga acctgagttt 60
gacagaggaa tgtaagatcc tggaaatcgg gttattggat tgggagagat tggggttgtt 120
<210> 190
<211> 120
<212> DNA
<213> Artificial sequence
<400> 190
gtcccgggag caccccactg accccgttgc gtctcgggca gctggtggcg cggtggcgtt 60
ctcccccgga aacctcagca ccagcagcag cgccagcagc accctgggca gccccgagaa 120
<210> 191
<211> 120
<212> DNA
<213> Artificial sequence
<400> 191
tgaggagcat atcctgtcct tcgagaccat cgacaagatg cgccgcgcca gctcctactc 60
ctccctcaac tccctcatcg gtgagtccgc ctgccccttt ctgcttccga ggggccccga 120
<210> 192
<211> 120
<212> DNA
<213> Artificial sequence
<400> 192
gccttaggag cctcattggc agctactttc cactaaaaca tcttccattt ctctctctca 60
ggagtttcct ttaacagtgt ttacactcag atttggagag tcctgctgca cctggctgct 120
<210> 193
<211> 120
<212> DNA
<213> Artificial sequence
<400> 193
gacccctatc cagaggtctc ggacgtggcc atgaaagtac tcaacagcat cgcctacaag 60
gtacgtgccg ggcgctcccc accgcgctcc agctgctggt tctcggttac gagtatgcat 120
<210> 194
<211> 120
<212> DNA
<213> Artificial sequence
<400> 194
tgcccctagg ccaccgtgaa cgcccggccg cagcgcgtcc tggacacctc ctccctcacg 60
cagtcggccc ccgccagccc caccaacaag ggcgtgcaca tccaccaggc ggggtaagtg 120
<210> 195
<211> 120
<212> DNA
<213> Artificial sequence
<400> 195
tgcagtgttt gtttttcttc caatggcagg ggctcccctc cggcgtccag caccagcagc 60
tccagcctga ccaacgatgt ggccaagcag ccggtcagcc gagacttgcc ttctggccgg 120
<210> 196
<211> 120
<212> DNA
<213> Artificial sequence
<400> 196
ccgggcacca caggccccgc tggggcgcag tacacccctc actcccacca gttcccccgg 60
acacggaaga tgttcgacaa gggcccagag caggtacggg agcccggctg cctggtgatc 120
<210> 197
<211> 120
<212> DNA
<213> Artificial sequence
<400> 197
tgaagactgc ggacgacgcg gacgatgctg ctggacacaa aagtttcatc tccgccacgg 60
tgcagacggg gttctgcgac tggagcgccc gctattttgc ccagcccgtc atgaaggtgc 120
<210> 198
<211> 120
<212> DNA
<213> Artificial sequence
<400> 198
tgttgaagat cccagaagag cacgacctgg agagtcagat ccgcaaggag cgggagtggc 60
ggttcctgcg aaacagccgt gtcaggaggc aggcccagca agtcattcag aagggtaagg 120
<210> 199
<211> 120
<212> DNA
<213> Artificial sequence
<400> 199
caggcattac gagattggac gaccaaatat ttctgaacag gaaccccggc gtcccctctg 60
tggtgaaatt ccaccccttc acgccgtgca tcgccgtagc cgacaaggac agcatctggt 120
<210> 200
<211> 120
<212> DNA
<213> Artificial sequence
<400> 200
tttgcagttt cctaatgact tttttattcc tctcttcttc ccttaagctt ttgggactgg 60
gagaaagggg agaagctgga ttatttccac aatgggaacc ctcggtacac gagggtcact 120
<210> 201
<211> 120
<212> DNA
<213> Artificial sequence
<400> 201
gccatggagt atctgaacgg ccaggactgc tcgcttctgc tgacggccac aggtgagcgg 60
ggtttgcaca gccaggattg gaagccaggg tctggaggag tggcggggag ggtgtgtgat 120
<210> 202
<211> 120
<212> DNA
<213> Artificial sequence
<400> 202
ctccccagac gatggtgcca tcagggtctg gaagaatttt gctgatttgg aaaagaaccc 60
agagatggtg accgcgtggc aggggctctc ggacatgctg ccaacgacgc gaggtgggtc 120
<210> 203
<211> 120
<212> DNA
<213> Artificial sequence
<400> 203
tctccaggag ctgggatggt ggtggactgg gagcaggaga ccggcctcct catgagctca 60
ggagacgtgc ggatcgtccg gatctgggac acagaccgtg agatgaaggt gcaggtaacc 120
<210> 204
<211> 120
<212> DNA
<213> Artificial sequence
<400> 204
gcagggaggg tggctcggtg ccccggtctt caccgggctg cctgtgtttg gctctaggac 60
atccctacgg gcgcagacag ctgtgtgacg agtctgtcct gtgattccca ccgctcactc 120
<210> 205
<211> 120
<212> DNA
<213> Artificial sequence
<400> 205
atcgtggctg gcctcggtga cggctccatc cgcgtctacg acagaaggat ggcactcagc 60
gaatggtacc ttgaccctgt cctctccctc cccgagtgct ggcagggtac cttccaggtg 120
<210> 206
<211> 120
<212> DNA
<213> Artificial sequence
<400> 206
tgccccctcc cctacagccg cgtcatgacg taccgggagc acacagcctg ggtggtgaag 60
gcctccctgc agaagcgtcc cgacggccac atcgtgagtg tgaggtgagg agcgccgata 120
<210> 207
<211> 120
<212> DNA
<213> Artificial sequence
<400> 207
agcgtcaatg gagatgtgcg catctttgat ccccggatgc ctgagtcggt aaatgtgctt 60
cagatcgtga aggggctgac ggccctggac atccaccccc aggcggacct gatcgcatgg 120
<210> 208
<211> 120
<212> DNA
<213> Artificial sequence
<400> 208
cggcctgtcc ccgcggtctc ggcatctgcg cccatcttcc atcctcaaag aagagggcag 60
tgatgccggg accctttctc tccccacagt ggctccgtca atcagttcac cgccatctac 120
<210> 209
<211> 119
<212> DNA
<213> Artificial sequence
<400> 209
aacagcagcg gagagctcat caacaacatc aagtactacg acggcttcat gggccagcgg 60
gtcggcgcca tcagctgcct ggccttccac ccgcactggg tcagtgtggc ggtgggtgg 119
<210> 210
<211> 120
<212> DNA
<213> Artificial sequence
<400> 210
tgctcacccc ttctctctcc ttgcagcctc acctggccgt gggaagcaac gactactaca 60
tctccgtgta ctcggtggag aagcgtgtca gatagcggcg tgacccgggc ccaccaggcc 120
<210> 211
<211> 120
<212> DNA
<213> Artificial sequence
<400> 211
ctgttgcact tgagtgtgag ttgcgggtgg cagcagaact aaggtacacc cctaggattg 60
caccataaaa taaggagaca actgagaggt gagatgcaca ggtggaaaat gccttgtact 120
<210> 212
<211> 120
<212> DNA
<213> Artificial sequence
<400> 212
tcccctgagc tgatgagatt gcatgtatgg aagaaattat cttagagtaa gagtaaagga 60
tcccagtcag gggacctcca cccagcagcg caactgtaaa atatatcacc atgtgattaa 120
<210> 213
<211> 120
<212> DNA
<213> Artificial sequence
<400> 213
gaaagctatc agaacaagca agttggatga cctgattaag ttcacagaaa aagtggggga 60
tttctaaggc tgtgcagaag gacagccgta ctaccattaa gatttgtagc aaggaataca 120
<210> 214
<211> 120
<212> DNA
<213> Artificial sequence
<400> 214
gagcactcat ggtccaggat gctagaacca gcagtccaca gaggtgaggg ttcataatga 60
ccatgtagtg cagggggtga cagatggcca caaaccggtc ataggccatc acggacagga 120
<210> 215
<211> 120
<212> DNA
<213> Artificial sequence
<400> 215
ggaagttttc aaatccagca aagagtatga aaaaatacat ctgcatgagg caggctatgt 60
aggtgatgac tttgttctgt gtctggatgt tcatcagcat ttttggaatg gtggtggaag 120
<210> 216
<211> 120
<212> DNA
<213> Artificial sequence
<400> 216
taacacaaat gtcagcaaag gacaggttgg agaggaagaa gtacatgggg gtgtggaggt 60
gggagtctga gattgtggcc aggatgatga gcaggttccc gagcacagtg accaggtaca 120
<210> 217
<211> 120
<212> DNA
<213> Artificial sequence
<400> 217
tggacaggaa cagcccaaag aggaagggtt gcagtccagg ttcttgtgaa aatcccagaa 60
gaagaaattc tgaaatttgt gtatcatttc ctggttccat ttgattgaag tgactatcag 120
<210> 218
<211> 120
<212> DNA
<213> Artificial sequence
<400> 218
agagagagag agaaagaggg aaacgagaca ggcatgcatt gctaaccttt cagaaatacc 60
atcatttata ttttatagcc aataaattat cttacatttt gtgtatgaat ctggttctct 120
<210> 219
<211> 119
<212> DNA
<213> Artificial sequence
<400> 219
ttaagggatc tccatgtcat ctctgattag gaactccttc ccactctcag ccttaactga 60
agacagcgta cattctgcag ttagaataag aacttctggc tgggcacggt ggcttatgc 119
<210> 220
<211> 120
<212> DNA
<213> Artificial sequence
<400> 220
gatgggacat ggtgtcagag gatgttgcag aataagggga cctggctgtc aaaacatcaa 60
gggacaaagt gtccatctgt ctagatgctg ttgcacctgc actttattaa ctggactaga 120
<210> 221
<211> 120
<212> DNA
<213> Artificial sequence
<400> 221
tgttgcacct gcactttatt aactggacta gaaaggtgtc acatcaattg aaatgcatcc 60
cctctttatt tccttctggt gtcttgggtt ctggcctctg tttcattggt ctcctttata 120
<210> 222
<211> 119
<212> DNA
<213> Artificial sequence
<400> 222
ttctggcctc tgtttcattg gtctccttta taataaaata tattgaggcc aggtccctct 60
tgctctgaaa cctgctgtca ctccaaagac atgcagccac acactcactc acacacaca 119
<210> 223
<211> 120
<212> DNA
<213> Artificial sequence
<400> 223
catgagctac tgcatttggc tcaaggaaag cttttaatat cagattcttt tttccctaca 60
gaatgtagaa ttggtacaag aaattccagt tcacaagtct gtatacacat gtaaacttgg 120
<210> 224
<211> 120
<212> DNA
<213> Artificial sequence
<400> 224
caagaaattc cagttcacaa gtctgtatac acatgtaaac ttggacttcc attttggtat 60
gactgttttc agccctgtgt ttaaagaaac ataaaagagc tgttcaataa acagagtgag 120
<210> 225
<211> 119
<212> DNA
<213> Artificial sequence
<400> 225
gtgtttaaag aaacataaaa gagctgttca ataaacagag tgagagaaat taaacagacc 60
ccagtaagga aaaagtctaa taaactcagg taatccctca gcaaaaggac aaaaataca 119
<210> 226
<211> 120
<212> DNA
<213> Artificial sequence
<400> 226
ctggggatct tataggatgg aaaataaaag cgaccctggt tatggttaca tttcagcttc 60
cattgtatct cctcgatgcc tggcaaaata aataaaagat caaaaataaa gaattctgac 120
<210> 227
<211> 120
<212> DNA
<213> Artificial sequence
<400> 227
tgcctggcaa aataaataaa agatcaaaaa taaagaattc tgacatcttt attgacttca 60
tggtttttaa tttaacaata gtatttaatt atgtcggttt taatgattat taaagtttta 120
<210> 228
<211> 119
<212> DNA
<213> Artificial sequence
<400> 228
aatagtattt aattatgtcg gttttaatga ttattaaagt tttagctttc cttttggaac 60
tagaaataag taaataaagt tatccaaaag acaagactgt ggagtcctaa ttagggaaa 119
<210> 229
<211> 120
<212> DNA
<213> Artificial sequence
<400> 229
gatagctgct acccatgaca aaaactaatg ataccttttt tggcaacaaa ggtacagatc 60
gcatatccta atagaaaaat ggagcaaaga gaataagcca acaattcaca aaaagaaaca 120
<210> 230
<211> 120
<212> DNA
<213> Artificial sequence
<400> 230
taagccaaca attcacaaaa agaaacatac aaacaatgac aagatttggg catttttata 60
tgaaacagta aaagactcat ttttttctgt gcctattcca tatttacttg atcaattggt 120
<210> 231
<211> 120
<212> DNA
<213> Artificial sequence
<400> 231
tattccatat ttacttgatc aattggtttt tatatgaatt gagagtggat gaagcagtcc 60
ctttctcact tttcttgtat ttcctgtgtg gcctttattt tattttatta ttttattatt 120
<210> 232
<211> 120
<212> DNA
<213> Artificial sequence
<400> 232
gcctcagtag ggcctccaca cggcctcctc caggcgcgcg gagggcgcca tgttggtggg 60
ggagttgctg tggctgccct cggcctccac cacgtcgctc tggttcggga ggctggggtt 120
<210> 233
<211> 120
<212> DNA
<213> Artificial sequence
<400> 233
gagcagcgcg gagccggtgg aggcgttggt gcagggcggc aggatgcgcg gcggcgagcg 60
ctcgccgccc accatggaga actggtagga gccggccgag gcgccgtagt acaggtggta 120
<210> 234
<211> 120
<212> DNA
<213> Artificial sequence
<400> 234
ggagggcgag ctggcttgga acgggcctcc ctgcgcttgc gacgagccgg ggtagggcgg 60
cggcaggtag gtgtggtagc gcgtggccga gcccatggcc gacatgccga tgccgatgcc 120
<210> 235
<211> 120
<212> DNA
<213> Artificial sequence
<400> 235
cgaggtgacc ggcgtcgggg agtaggtgaa ggcgcctgga tagtgcatgc gggggtcgga 60
gatggagggc agcgcgggga actggcgcgg gtcgctgaac gctgtcaggt cgggtgccgc 120
<210> 236
<211> 120
<212> DNA
<213> Artificial sequence
<400> 236
ccagctcagc tgcaaagaat gtgttttcaa gtggcttact tgagagtcga ctggaaagtt 60
ctgcagagag ggttgtcatg ccgctggcac gtccaggtga aatgggcgtt gctgggtgca 120
<210> 237
<211> 120
<212> DNA
<213> Artificial sequence
<400> 237
cagaaggaga ggcaatggat cccaggtatt ggtaggactg atcgtaggac cacggtgggg 60
atggttggat ctgccttgta tctgaagaga atcagaaagg tcaattatat gtaaagtggg 120
<210> 238
<211> 120
<212> DNA
<213> Artificial sequence
<400> 238
ctatcctggc tggggagagg gatggacaga agaactgact tctgccttaa catctccagg 60
gtgctgtgtc ttcctctgag ggagccatgt gtgaccaggg agaaagttcg aaaataagga 120
<210> 239
<211> 120
<212> DNA
<213> Artificial sequence
<400> 239
gcagtgggct ccatctggta cttaccctgc atctgactct gaggctgagg gttaaaggca 60
gtggagtggt tcagggaggc acgagggttg ggcgtggggg ctgggtggtg tgggctgacc 120
<210> 240
<211> 120
<212> DNA
<213> Artificial sequence
<400> 240
ctcatggctg tgcgccgcag ctgctccagt tcactgagcc gctcggaaaa ggacaagctc 60
ccgggcttgg tctgatcatc tagtttctgc cgatgtccta ttgtggggag cagggagggg 120
<210> 241
<211> 120
<212> DNA
<213> Artificial sequence
<400> 241
gcacttactt cgaggttctc ggggcccatc cactgtgatt ttgatggctc tgtggtaggt 60
ggcgacttgc ggtgggtttg tgaagacagt gatggtcaga gtgaagcttt tccctgtggg 120
<210> 242
<211> 120
<212> DNA
<213> Artificial sequence
<400> 242
ccatgaaacg tgtttcaagc atagttttga cagataacgt acctcttcca cttcgaccga 60
caaacctgag gtcattaaat cttgcaacct ggttcttcat ggctgcggta gcatttctca 120
<210> 243
<211> 120
<212> DNA
<213> Artificial sequence
<400> 243
gctcagccga gtagttttca tcattgccag ccatcacagt gaccagagtg ccatctggaa 60
catcccctag ggccaccacc taaacaccag tcaaaggaca aatgcagaca tcagggatgt 120
<210> 244
<211> 120
<212> DNA
<213> Artificial sequence
<400> 244
cgccggcctc cgcctgtcct cccaccaccc tctccgggcc agtaccttga aagcgatggg 60
cagggtcttg ttgcagcgcc agtgcgtagg cagcacggag cagaggaagt tggggctgtc 120
<210> 245
<211> 120
<212> DNA
<213> Artificial sequence
<400> 245
ggtgcgcacc agctcgcccg ggtggtcggc cagcacctcc accatgctgc ggtcgccgct 60
cctcagcttg ccggccaggg cagcgccggc gtccggggcg cccagcggca acgcctcgct 120
<210> 246
<211> 120
<212> DNA
<213> Artificial sequence
<400> 246
catcttgcct gggctcagcg cggtggaagg cggcgtgaag cggcggctcg tgctggcatc 60
tacggggata cgcatcacaa caagccgatt gagttaggac cctgcaaaca gctcctacca 120
<210> 247
<211> 120
<212> DNA
<213> Artificial sequence
<400> 247
tgtgtacttt atttaaaaat ataacttgga atttaacata ccgtggacgt ctctagaagg 60
attcattcca agtatgcatt ctgaaataac agaaagtagg aaaataaaag taatgcaagt 120
<210> 248
<211> 120
<212> DNA
<213> Artificial sequence
<400> 248
agtagatatt acaagaccag catgtactca cctctcatga agcactgtgg gtacgaagga 60
aatgactcaa atatgctgtc tgaagccatc gcttcctcct gaaaatgcac cctcttctga 120
<210> 249
<211> 120
<212> DNA
<213> Artificial sequence
<400> 249
gggggtggtg tccccagccg gtttgggggg tgcgttgccc ggagacggaa agtttgggag 60
cccgagcagg ctcggctgca gcctcgggga gggggtccag cgggtggcgg ccctggggat 120
<210> 250
<211> 119
<212> DNA
<213> Artificial sequence
<400> 250
ggggaaggag caggagctgc tggaggcggc ccgcaccggg cacctcccgg cggtggagaa 60
gctgctgtcc gggaagcggc tctcctcagg ctttgggggc ggcggcggcg gtggctctg 119
<210> 251
<211> 120
<212> DNA
<213> Artificial sequence
<400> 251
gcggcggcgg cggcggcggc ctcggctctt ccagccaccc cctctccagt ctgctcaggt 60
gggtacgcgc cagggccggg ccgctgcctg cagacccttt ctcccccacc cgtctcttgg 120
<210> 252
<211> 120
<212> DNA
<213> Artificial sequence
<400> 252
cttttttttt tccctgatag catgtggaga gggccaaatg tgaactgtgt tgacagcact 60
ggctacacac ccctgcacca tgctgctttg aatggccata agtaagtatc aatgtactac 120
<210> 253
<211> 120
<212> DNA
<213> Artificial sequence
<400> 253
cttctgagtt ctaccaactc atgattgatt tttgccttct agggatgtgg tcgaggttct 60
tctgaggaac gatgcgctga ccaacgtggc tgactcaaaa ggctgctacc ctctgcattt 120
<210> 254
<211> 120
<212> DNA
<213> Artificial sequence
<400> 254
ggcagcctgg aaaggagatg cccagatagt gcggttgctc atccatcaag ggccttcaca 60
caccagagtc aatgaacagg tcggaaggaa gggaggcttt ccttcctcca ttcagctata 120
<210> 255
<211> 120
<212> DNA
<213> Artificial sequence
<400> 255
tttctgatgt gatctgcttg ttaacccttt agaacaatga caacgagaca gccctgcatt 60
gtgcagcgca gtatggccac acagaggtgg tgaaggtgct cttagaggag ctgacggacc 120
<210> 256
<211> 120
<212> DNA
<213> Artificial sequence
<400> 256
ccaccatgcg caacaacaaa ttcgagaccc ctttggacct ggcagcactg tacgggcgac 60
tggaggtggt gaaaatgctc cttaatgcac accccaacct cctgagctgc aacactaaga 120
<210> 257
<211> 120
<212> DNA
<213> Artificial sequence
<400> 257
agcacacccc tctgcacttg gcagcaagga atggccacaa agccgtggtc caggtcctcc 60
tcgatgctgg catggacagc aactaccagg tagcagggcg ggtgggggct cctccaatct 120
<210> 258
<211> 120
<212> DNA
<213> Artificial sequence
<400> 258
ctgcgctctc gtttgctttc cagacggaga tgggcagtgc tttgcatgag gctgctttgt 60
ttggcaagac cgatgtggtg caaatcctgc tggctgcagg tgagaggtcg gcagcgctct 120
<210> 259
<211> 120
<212> DNA
<213> Artificial sequence
<400> 259
cctttctagg aactgacgtc aacataaaag ataaccatgg actgactgcc ctagacactg 60
ttcgggaact gccttctcaa aagagccagc aaatagcagc attaattgaa ggtatcatcc 120
<210> 260
<211> 120
<212> DNA
<213> Artificial sequence
<400> 260
tccatgtaga tcacatgact ggaaaaagaa gtacaaaaga agtagataaa acccccccac 60
cccagccacc tctcatctcc agtatggact ccatatcaca gaagtctcag ggtaaggtaa 120
<210> 261
<211> 120
<212> DNA
<213> Artificial sequence
<400> 261
ttgccctcca tcctcctctc aggtgacgtg gagaaagcag tgactgaact gattatagat 60
tttgatgcaa atgctgaaga agagggtccc tacgaagctc tgtataatgc catctcctgc 120
<210> 262
<211> 120
<212> DNA
<213> Artificial sequence
<400> 262
cattcgttgg acagcatggc cagcgggcga tcatctgacc aagactccac gaacaaggag 60
gctgaggcag caggagtgaa acctgctgga gtgaggcctg tatgtgaccc ggggcttaca 120
<210> 263
<211> 120
<212> DNA
<213> Artificial sequence
<400> 263
tttctcttct gcagagggaa cgtccaccac ctccagcaaa gccaccgccc gatgaagagg 60
aagaagacca catagataag aagtattttc ccttgacagc ttctgaggta gagggttgtg 120
<210> 264
<211> 120
<212> DNA
<213> Artificial sequence
<400> 264
gactgcctgt cttggtatta gccacatgca caagatacac tggatgtttc tctcccttag 60
gttctgtcca tgagacctag gattcatggg agtgcagccc gggaagaaga cgaacaccct 120
<210> 265
<211> 120
<212> DNA
<213> Artificial sequence
<400> 265
tatgaactgt tgttaacagc agagacaaag aaagtggtgt tggtggatgg aaaaacaaaa 60
ggtacgttcc ccacaactcc tggcagaacc aactccaaag ggatttttaa agtttgtgat 120
<210> 266
<211> 120
<212> DNA
<213> Artificial sequence
<400> 266
ctccaagacc acaggcggag cagcagcagc cggagccagg actctgcgga ggggcaggac 60
gggcaggtcc cagagcagtt ctcaggcctc ctccacggct cctccccggt gtgcgaggtg 120
<210> 267
<211> 120
<212> DNA
<213> Artificial sequence
<400> 267
gggcaggacc ctttccagct gctctgtacc gctggccaga gccatccaga cgggtccccc 60
cagcagggcg cctgccacaa ggccagcatg cagctggagg agacgggtgt gcatgctcct 120
<210> 268
<211> 120
<212> DNA
<213> Artificial sequence
<400> 268
ggagcctccc agcccagtgc cctggaccag agcaagagag tgggctacct cacaggcctg 60
cccaccacca acagccgctc gcaccctgaa actttgactc acacagcatc tccgcaccct 120
<210> 269
<211> 120
<212> DNA
<213> Artificial sequence
<400> 269
ggtggtgctg aggaaggaga ccggagtggg gccaggagcc gagcgcctcc cactagcaaa 60
cccaaagctg aactcaaact cagccgcagc ttgtccaagt ctgactctga tctcctgacc 120
<210> 270
<211> 120
<212> DNA
<213> Artificial sequence
<400> 270
tgctcaccca cagaggacgc taccatgggg agtcggagtg agtccttatc caactgcagc 60
attgggaaga aaaggctaga gaagtcaccc tccttcgcct cggagtggga tgaggtaagg 120
<210> 271
<211> 120
<212> DNA
<213> Artificial sequence
<400> 271
tcctctttgt tctttcttcc atttcagatt gagaaaatca tgagttctat tggagaaggg 60
attgactttt ctcaggaacg gcagaagatc tcaggtaccg tactaagtgg ccattttccc 120
<210> 272
<211> 120
<212> DNA
<213> Artificial sequence
<400> 272
tcatcaggtt tacggacgct ggagcagagt gtcggggagt ggctggagtc gattgggctg 60
cagcagtatg agagcaagtt gcttctgaat ggctttgacg atgtccactt cctggtaagt 120
<210> 273
<211> 120
<212> DNA
<213> Artificial sequence
<400> 273
tctgctctca ggggtctaat gtgatggaag agcaggacct gcgggacatc ggcatcagcg 60
acccacagca ccggcggaag ctgctccagg cggcacgctc cctacccaag gtgaccatcg 120
<210> 274
<211> 120
<212> DNA
<213> Artificial sequence
<400> 274
gtgagtgctg agatgtcagt ttcacctccc tccttgccct gtaggtgaag gctctgggtt 60
atgacgggaa cagcccccct agcgtgccct cctggctgga ctccctgggg ctgcaggact 120
<210> 275
<211> 120
<212> DNA
<213> Artificial sequence
<400> 275
acgtccattc cttcttgtca agtggttaca gctccattga caccgtgaag aacctctggg 60
agctagagct cgtcaatgtg agtagtccct gcgtggcccg gcctggactc tccagaagtc 120
<210> 276
<211> 120
<212> DNA
<213> Artificial sequence
<400> 276
cacccaggtc ctgaaggtcc agctgctcgg ccatcgcaag cgcatcatcg cctccctcgc 60
agacagaccg tacgaggagc cgccccagaa gccccccaga ttctcccagc tgagggtgag 120
<210> 277
<211> 120
<212> DNA
<213> Artificial sequence
<400> 277
gagggttgca agttccacct ggatttttcc ctccacagtg ccaagatttg ctctcccaga 60
cgtcatcccc actgagtcag aatgattcct gcactgggcg gtcggcagat ctgctgctgc 120
<210> 278
<211> 120
<212> DNA
<213> Artificial sequence
<400> 278
ctccagggga cacaggcagg aggcgccatg acagtctcca tgaccctgcg gcaccctccc 60
gagcggagcg cttcaggatc caggtggggc agggggagtg gaggtgcagc caggctctac 120
<210> 279
<211> 120
<212> DNA
<213> Artificial sequence
<400> 279
aagcccatgt gctcctctgg agcaaggagc aggtgtccaa ttgcgtgtgt ttcgcaggag 60
gagcaccgtg aggccaagct gaccctgcgg cccccgagcc tggcagcccc ctacgcccca 120
<210> 280
<211> 120
<212> DNA
<213> Artificial sequence
<400> 280
gtgcagagtt ggcaacacca gccagagaaa ctcatcttcg agtcctgtgg ttatgaagcc 60
aatgtgagtt gctcccaccc tcccagcagg gccggcctcc ctgctccttc tcggccaggc 120
<210> 281
<211> 120
<212> DNA
<213> Artificial sequence
<400> 281
gcctggccac tgctcgcccc cacagtatct gggctccatg ctgatcaaag atctgcgagg 60
gacagaatcc acgcaagacg cctgtgccaa gatgcgggta gggtgcctgt gtgggctgga 120
<210> 282
<211> 120
<212> DNA
<213> Artificial sequence
<400> 282
ccccttgttt cctgtagaaa tctacggagc acatgaagaa gatccccacc atcatcctgt 60
ccatcacata caaaggtgtc aagttcatcg atgcctccaa caaggtgtgc tgcttacagg 120
<210> 283
<211> 120
<212> DNA
<213> Artificial sequence
<400> 283
ccagaggcat gcctgagcct gagaattcca gaacacggct ttccccagca gaacgtcatt 60
gcagagcacg agatccggaa catttcctgt gcggcccagg acccggagga cctctgtacc 120
<210> 284
<211> 120
<212> DNA
<213> Artificial sequence
<400> 284
tttgcctaca tcaccaagga cctgcagacc agccaccact attgccatgt gttcagcacc 60
gtggatgtgg tgggtggggt cctggggccg ggtggggcag gcttgggttg cagccctgag 120
<210> 285
<211> 120
<212> DNA
<213> Artificial sequence
<400> 285
accaggtgct taagtggtga tctgcttcct cccagaacct gacctacgag atcatcctga 60
cgctggggca ggccttcgaa gtggcctatc agttggccct gcaggcccag aagtccaggg 120
<210> 286
<211> 120
<212> DNA
<213> Artificial sequence
<400> 286
cgacgggcgc ctctgcagct gagatgattg aaacaaaatc ttccaaaccg gtgcctaagc 60
ctcgggtcgg cgtgaggaaa tccgcagtac gtgggcccca ctggccaaga tccccctctc 120
<210> 287
<211> 120
<212> DNA
<213> Artificial sequence
<400> 287
ttgtttggca gctggaacca cctgatatgg accaagatgc ccaatcccat gccagtgtct 60
cctgggttgt ggaccccaaa ccagactcta agcggagcct cagcaccaag tatgagacca 120
<210> 288
<211> 120
<212> DNA
<213> Artificial sequence
<400> 288
accgtccgca ggccccattg gttaaccctt tcctcccccc attgtttgct tctgccaagc 60
tgagccactg aggaaccaca ctgtgctgcg gaaacataga cgtgggggca taggctccca 120
Claims (10)
1. Use of a reagent for detecting a target region in the manufacture of a kit for predicting the risk of prognosis of squamous cell lung cancer, wherein the target region comprises: regions 1 to 127 shown in the following table;
。
2. The use of claim 1, wherein the reagent comprises any one of a probe, a primer and a chip.
3. The use of claim 2, wherein the probes comprise at least 1 probe of probes 1-288; the sequences of the probes 1-288 are respectively shown in SEQ ID Nos. 1-288.
4. An apparatus for predicting the prognostic risk of squamous cell lung cancer, comprising:
the prediction module is used for judging whether mutation occurs according to the sequence information of the corresponding target region in the genome of the sample to be detected so as to predict the prognosis risk of the sample to be detected;
wherein the target region comprises regions 1-127 shown in the following table;
。
5. The apparatus for predicting prognostic risk of squamous cell lung cancer according to claim 4, wherein the prediction module further comprises: and based on the sequence information, marking the sample to be detected with mutation in the target region as a high-risk sample, and otherwise, marking the sample to be detected as a low-risk sample.
6. The apparatus for predicting prognostic risk of squamous cell lung cancer according to claim 5, wherein the apparatus further comprises: and the acquisition module is used for acquiring the sequence information of the corresponding target region in the genome of the sample to be detected.
7. An electronic device, comprising: the system comprises a memory, a processor and a computer program stored on the memory and capable of running on the processor, wherein the processor executes the computer program to realize a method for predicting the lung squamous carcinoma prognosis risk, and the method comprises the steps of predicting the lung squamous carcinoma prognosis risk based on the acquired sequence information of the corresponding target region in the genome of a sample to be tested;
the target region comprises regions 1-127 shown in the following table;
。
8. The electronic device of claim 7, wherein the method comprises detecting with at least 1 probe of probes 1-288, and the base sequences of probes 1-288 are shown as SEQ ID Nos. 1-288 in sequence.
9. A computer-readable medium storing a computer program executable by a processor to implement a method for predicting a risk of lung squamous cancer prognosis, the method comprising predicting a risk of lung squamous cancer prognosis based on sequence information of a corresponding target region in a genome of a test sample obtained;
the target region comprises regions 1-127 shown in the following table;
。
10. The computer-readable medium of claim 9, wherein the method comprises detecting with at least 1 probe of probes 1-288, and the base sequences of probes 1-288 are shown as SEQ ID Nos. 1-288 in sequence.
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| US20140045915A1 (en) * | 2010-08-31 | 2014-02-13 | The General Hospital Corporation | Cancer-related biological materials in microvesicles |
| SG11201608403TA (en) * | 2014-04-28 | 2016-11-29 | Sigma Aldrich Co Llc | Epigenetic modification of mammalian genomes using targeted endonucleases |
| CN104975082B (en) * | 2015-06-05 | 2018-11-02 | 复旦大学附属肿瘤医院 | One group of gene and its application for assessing lung cancer for prognosis |
| CN108009400B (en) * | 2018-01-11 | 2018-07-06 | 至本医疗科技（上海）有限公司 | Full-length genome Tumor mutations load forecasting method, equipment and storage medium |
| CN109859801B (en) * | 2019-02-14 | 2023-09-19 | 辽宁省肿瘤医院 | Model for predicting lung squamous carcinoma prognosis by using seven genes as biomarkers and establishing method |
| CN109880910B (en) * | 2019-04-25 | 2020-07-17 | 南京世和基因生物技术股份有限公司 | Detection site combination, detection method, detection kit and system for tumor mutation load |
| CN110499364A (en) * | 2019-07-30 | 2019-11-26 | 北京凯昂医学诊断技术有限公司 | A kind of probe groups and its kit and application for detecting the full exon of extended pattern hereditary disease |
| CN110317875A (en) * | 2019-07-30 | 2019-10-11 | 苏州呼呼健康科技有限公司 | One kind methylated genes relevant to lung cancer and its detection kit |
| CN110957007B (en) * | 2019-11-26 | 2023-04-28 | 上海交通大学 | Multi-group analysis method based on tissue exosome phosphorylated proteome |
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