KR102422776B1 - Gene panel for biopsy anaylsis and method for personalized therapy using the same - Google Patents

Abstract

Provided are a gene panel for biopsy analysis and a method of providing information for personalized treatment using the same. According to this, cancer gene mutations can be detected with high sensitivity in a sample such as blood, and information on the detected cancer gene mutations can be used to provide personalized treatment.

Description

Gene panel for biopsy anaylsis and method for personalized therapy using the same}

It relates to a gene panel for biopsy analysis and a personalized treatment method using the same.

A tumor is a disease that occurs due to the failure of the regulation of cell proliferation. The main cause is a loss of control of cell function due to gene mutation. Because these DNA mutations are responsible for cancer development, cancer progression, metastasis, and cancer characteristics such as anticancer drug reactivity and resistance, analyzing the characteristics of cancer genes is very important in cancer treatment strategies. It can also provide patients with useful information to avoid unnecessary treatment.

Among next generation sequencing (NGS) techniques, targeted NGS is a technique for analyzing the DNA sequence of a specific gene part to be targeted. Target NGS has advantages of high sequencing depth, low cost, and efficient gene analysis in a short time. By using NGS analysis, it is possible to improve the anticancer treatment effect by applying precision medicine that analyzes the genetic characteristics of cancer occurring in a patient (Korea Patent Publication No. 10-2019-0093445).

Liquid biopsy is a method of analyzing DNA from body fluids such as blood by genetic testing. Liquid biopsy is attracting attention as an alternative to tissue biopsy in that it is faster and simpler than biopsy and can analyze mutations peculiar to tumor cells. In particular, cell-free DNA (cfDNA) and circulating tumor cell (CTC) DNA in blood are known to show cancer gene diversity better than tissue biopsy. In addition, the gene analysis method using blood has the advantage of being able to repeatedly and non-invasively collect during the treatment and treatment of patients, so that the latest disease state can be confirmed.

Therefore, there is a need to develop a high-sensitivity gene NGS panel for biopsy analysis, such as liquid biopsy, to detect cancer mutations with high sensitivity.

Genetic panels for biopsy analysis are provided.

A method of providing information for personalized treatment using a gene panel for biopsy analysis is provided.

One aspect provides a panel of genes for biopsy analysis comprising at least 10 contiguous polynucleotides selected from a nucleotide sequence identical to or complementary to a polynucleotide of at least one gene selected from the group consisting of:

ALK (ALK receptor tyrosine kinase), AR (androgen receptor), BAP1 (BRCA1 associated protein 1), BRCA1 (BRCA1 DNA repair associated), BRCA2 (BRCA2 DNA repair associated), BTK (Bruton tyrosine kinase), CDK4 (cyclin dependent kinase) 4), ERBB2 (erb-b2 receptor tyrosine kinase 2), ERBB3 (erb-b2 receptor tyrosine kinase 3), ESR1 (estrogen receptor 1), FGFR1 (fibroblast growth factor receptor 1), FGFR2 (fibroblast growth factor receptor 2), FGFR3 (fibroblast growth factor receptor 3), FGFR4 (fibroblast growth factor receptor 4), MYCN (MYCN proto-oncogene, bHLH transcription factor), NOTCH1 (notch receptor 1), PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), PTCH1 (patched 1), RET (ret proto-oncogene), STK11 (serine/threonine kinase 11), TP53 (tumor protein p53), TSC1 (TSC complex subunit 1), and TSC2 (TSC complex subunit 2) .

The term "biopsy" refers to a method of diagnosing or treating a disease by analyzing a nucleic acid fragment in a biological sample. The sample for the biopsy analysis may be blood, plasma, serum, urine, saliva, mucosal secretion, sputum, tears, tissue, FFPE (Formaldehyde Fixed Paraffin Embedded), or a combination thereof. The biopsy may be a liquid biopsy. The sample for the biopsy analysis may be cell-free DNA (cfDNA), circulating tumor cell (CTC)-derived DNA, or a combination thereof. cfDNA refers to fragments of degraded DNA in body fluids such as plasma. Circulating tumor cell-derived DNA refers to DNA fragments isolated from tumor cells in body fluids.

The term “gene panel” refers to a genetic mutation testing apparatus or method using the same in which genetic mutations for a plurality of target genes are configured as one panel.

A “polynucleotide” may be DNA or RNA. The polynucleotide may also be in single-stranded or double-stranded form. The polynucleotide may include natural nucleotides, analogs of natural nucleotides, nucleotides in which sugar, base or phosphate sites of natural nucleotides are modified, and combinations thereof (Scheit, Nucleotide Analogs, John Wiley, New York (1980)) ; Uhlman and Peyman, Chemical Reviews, 90:543-584 (1990)).

The polynucleotide may include nucleotides with genetic variation. The genetic variation may include mutation and genetic recombination. The mutation may be a point mutation, an insertion mutation, a deletion mutation, or a substitution mutation. The genetic variation may be associated with the risk of developing a disease such as cancer, diagnosis, prognosis, and monitoring. The genetic mutation may be related to the therapeutic target of the therapeutic drug.

The gene panel may include 10 or more contiguous polynucleotides selected from the same or complementary nucleotide sequence to each polynucleotide of the gene. For example, for BRCA2, in the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 8, the nucleotide at position 7469 from the 5'-end may be "C or T". In this case, the gene panel comprises a "C or T" nucleotide at the 7469th position from the 5'-end thereof in the nucleotide sequence of SEQ ID NO: 8, and 10 or more contiguous polynucleotides selected from the nucleotide sequence of SEQ ID NO: 8 or It includes a single-stranded polynucleotide complementary thereto.

The polynucleotide of the gene may include a polynucleotide consisting of a nucleotide sequence of SEQ ID NO: selected from the group consisting of SEQ ID NOs: 1 to 51.

The 10 or more contiguous polynucleotides may be primers, probes, or antisense nucleic acids. The primer is a single-stranded single-stranded DNA that can serve as an initiation point for template-directed DNA synthesis under suitable conditions (e.g., four different nucleoside triphosphates and a polymerization enzyme) in a suitable buffer at a suitable temperature. oligonucleotides. The length of the primer may be 15 to 30 nucleotides (nucleotide: nt). The probe refers to a natural or modified monomer comprising deoxyribonucleotides and/or ribonucleotides capable of hybridizing to a specific polynucleotide sequence or a linear oligomer having a bond. For example, the probe may be single-stranded to increase hybridization efficiency. The length of the probe may be 5 to 100 nt, 10 to 90 nt, 15 to 80 nt, 20 to 70 nt, or 30 to 50 nt. The antisense nucleic acid (antisense oligonucleotide)" refers to a molecule that inhibits translation of a target gene as a short single DNA molecule that interacts with messenger RNA (mRNA). The antisense nucleic acid has a length of 10 nt or more, more specifically may be 10 to 200 nt, 10 to 150 nt, or 10 to 100 nt, but an appropriate length may be selected to increase detection specificity.

The primer, probe, or antisense nucleic acid is a nucleotide sequence that is completely complementary to the target sequence comprising the genetic variation site, or substantially within a range that does not interfere with specific hybridization to the target sequence comprising the genetic variation site. and may have a complementary nucleotide sequence. In addition, the primer, probe, or antisense nucleic acid may have modified nucleotides within a range that does not impair specific hybridization to a target sequence including a genetic mutation site.

The polynucleotide may be a polynucleotide labeled with a detectable label. The detectable label is a label material capable of generating a detectable signal, and may be a label material capable of generating a detectable signal including a fluorescent material, for example, substances such as Cy3 and Cy5. The detectable label may confirm the hybridization result of the nucleic acid.

The gene panel may be for targeted treatment of cancer. The cancer is ovarian cancer, cervical cancer, endometrial cancer, uterine sarcoma, vulvar cancer, breast cancer, skin cancer, head and neck cancer, pancreatic cancer, lung cancer, colorectal cancer, colorectal cancer, stomach cancer, prostate cancer, bladder cancer, urethral cancer, liver cancer, kidney cancer , skin cancer, cerebrospinal cancer, brain cancer, thymoma, mesothelioma, bronchial cancer, nasopharyngeal cancer, laryngeal cancer, esophageal cancer, biliary tract cancer, testicular cancer, germ cell tumor, thyroid cancer, parathyroid cancer, lymphoma, myelodysplastic syndromes (MDS), myelofibrosis (myelofibrosis), acute leukemia, chronic leukemia, multiple myeloma, endocrine system cancer, and may be selected from the group consisting of sarcoma. Since the gene panel is based on cancer genes for each individual patient, it can effectively target various diseases caused by genetic mutations, and can enhance the therapeutic effect of cancer through personalized gene therapy.

Another aspect comprises the steps of detecting a genetic variation of a genetic panel according to an aspect from a biological sample isolated from the subject; And it provides a method for providing information for personalized treatment, comprising the step of setting a gene in which the detected genetic mutation has occurred as a therapeutic target of the individual.

The method includes detecting a genetic variation in a genetic panel according to an aspect from a biological sample isolated from an individual.

The genetic panel and genetic variation are the same as described above.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat or cat. The subject may be an individual who has or is likely to have cancer.

The biological sample may be blood, plasma, serum, urine, saliva, mucosal secretions, sputum, tears, tissue, Formaldehyde Fixed Paraffin Embedded (FFPE), or a combination thereof.

The method includes setting a gene in which the detected genetic mutation has occurred as a therapeutic target for the subject.

The method may further include isolating the nucleic acid from the biological sample isolated from the subject. The method for isolating a nucleic acid from the biological sample may be performed by a method known to those skilled in the art. The nucleic acid may be cell-free DNA (cfDNA), circulating tumor cell (CTC)-derived DNA, or a combination thereof. The length of the isolated nucleic acid fragment is from about 10 bp (base pair) to about 2000 bp, from about 15 bp to about 1500 bp, from about 20 bp to about 1000 bp, from about 20 bp to about 500 bp, from about 20 bp to about 200 bp , or from about 20 bp to about 100 bp.

The detecting of the genetic mutation may include performing next-generation sequencing (NGS) by adding the isolated nucleic acid to the gene panel according to an aspect. The term “next-generation sequencing (NGS)” may be used interchangeably with massive parallel sequencing or second-generation sequencing. Next-generation sequencing is a technique for sequencing nucleic acids of millions of fragments simultaneously. Next-generation sequencing is, for example, 454 platform (Roche), GS FLX Titanium, Illumina MiSeq, Illumina HiSeq, Illumina Genome Analyzer, Solexa platform, SOLiD System (Applied Biosystems), Ion Proton (Life Technologies), Complete Genomics, Helicos Biosciences Heliscope, Pacific Biosciences' Single Molecule Real Time (SMRT™) technology, or a combination thereof, in parallel fashion.

The detecting of the genetic mutation may further include detecting the genetic mutation by comparing the nucleotide sequence information obtained as a result of performing next-generation sequencing with the nucleotide sequence information of the normal control. The normal control may be a sample isolated from a non-cancerous individual or a sample isolated from a cancer-free region. The normal control may be a peripheral blood mononuclear cell (PBMC) of the subject.

The information for the personalized treatment may be cancer diagnosis, cancer prognosis prediction, cancer progress monitoring, or a combination thereof.

The method indicates that the detected genetic mutations are T7469C mutation in BRCA2 , C3508G mutation in ERBB2 , C3149T mutation in ERBB2 , A1451G mutation in ESR1 , G1162A mutation in FGFR4 , C3785T mutation in PTCH1 , C1062G mutation in PTCH1, and C3943T mutation in STK11T . When it is selected from the group consisting of a T3524C mutation of TSC2 , the method may further include determining that the subject has or has a high probability of having gynecologic cancer. By precisely detecting the genetic mutation, it is possible to diagnose cancer, set a target gene for cancer treatment in an individual, perform effective targeted treatment, predict a prognosis, and monitor the progress of cancer.

According to a gene panel for biopsy analysis and a method of providing information for personalized treatment using the same, cancer gene mutations can be detected with high sensitivity in a biological sample such as blood, and personalized through information on the detected cancer gene mutations It can be used to provide treatment.

1 is a schematic diagram showing a next-generation genome sequencing method using blood.
Figure 2a is a graph of allele frequencies (%) detected with the NGS panel versus allele frequencies of the reference standard, and Figure 2b is a graph of the mean allele frequencies (%) detected with the NGS panel versus allele frequencies (%) of the reference standard ( %) (R ² =0.9955).
As shown in FIGS. 2A and 2B , mutations in NRAS (Q61K, A59T), EGFR (E756-A750, T790M, L858R), and KRAS (G12D) were detected up to 1% allele frequency by the manufactured NGS panel. . Mutations in EGFR (V769-D770insASV) and PIK3CA (E545K) were detectable even at 0.1% allele frequency (in FIG. 2b, R ² =0.9955).

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes of the present invention, and the scope of the present invention is not limited to these examples.

Example 1. Highly sensitive detection of mutations in cell-free DNA or circulating tumor cell DNA in blood samples

1. Preparation of DNA Samples

From patients (total of 20) diagnosed with gynecological cancer: ovarian cancer (9 patients), cervical cancer (4 patients), endometrial cancer (3 patients), uterine sarcoma (3 patients), or vulvar cancer (1 patient) Blood samples were collected.

Plasma was separated from 7 mL of collected whole blood by gradient centrifugation using Ficoll-Paque™ PLUS (GE Healthcare). Cell-free DNA (cfDNA) was extracted from the separated plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen).

For the peripheral blood mononuclear cell (PBMC) layer, circulating tumor cells (CTC) were collected using the CD-PRIME™ system (Clinomics Inc.). The CD-PRIME™ system is a centrifugal force-based size-sorting CTC separation platform capable of separating and concentrating CTCs from PBMC layers. According to the manufacturer's instructions, an equal volume of Ficoll-Paque™ PLUS solution was added to the peripheral blood, and the mixture was centrifuged at 800 x g. After centrifugation, the PBMC fraction was recovered from the blood cell fraction and the CD-PRIME™ system was used for CTC enrichment. CTCs were identified by identifying DAPI+, EpCAM/CK+, and CD45-, which are biomarkers of CTCs, and the correct number of CTCs was calculated under a fluorescence microscope at x40 magnification.

Using CD-PRIME™, concentrated CTCs were placed on the membrane on CD-CTC-discs. The membrane was transferred to a collection tube, and CTC DNA (ctcDNA) was extracted using the QIAamp DNA Micro Kit (Qiagen).

Meanwhile, as a normal control, DNA was extracted from PBMCs.

The extracted DNA was subjected to whole genome amplification (WGA) using the REPLI-g Mini Kit. DNA was analyzed qualitatively and quantitatively using a Qubit 3.0 fluorometer (Thermo-Fisher Scientific). In addition, the level of genomic DNA contamination in the collected DNA samples was verified with a 2100 Bioanalyzer (Agilent).

2. Genome Analysis

10 ng of cfDNA or ctcDNA prepared as described in Example 1.1 was used for library construction. Ion Ampliseq Library Kit 2.0 (Thermo-Fisher Scientific) was used and the library was prepared according to the manufacturer's instructions.

For sample multiplexing, the Ion Xpress Barcode Adapters Kit (Thermo-Fisher Scientific) was used, and the prepared library was purified with Agencourt AMPure XP reagent (Beckman Coulter). After that, the library was quantified using a Qubit 3.0 fluorometer and 2100 Bioanalyzer.

A template for the library was prepared using an Ion Chef Instrument (Thermo-Fisher Scientific) equipped with an Ion 540 Chef Kit (Thermo-Fisher Scientific). The multiplexed template was prepared on an Ion S5 XL system (Thermo-Fisher Scientific). A sequencing was performed.

3. Production of next-generation sequencing panel

Next generation sequencing capable of detecting single nucleotide variant (SNV), insertion-deletion polymorphism (INDEL), and copy-number variation (CNV) in the genome : NGS) panel was manufactured. As a candidate gene of the panel, it was selected from genes that could be used as a target anticancer drug when a mutation was found, which is related to the target anticancer drug. Arm panels were designed using Ampliseq Designer (5.4.1, Thermo-Fisher Scientific). A panel was prepared so that a total of 1,355 amplicons were made from two pairs of primer pools. The average size of the amplicon is about 125 bp to 175 bp, and the length of the nucleic acid covered by the amplicon is about 136.75 kb. Information on actionable genes used in the NGS panel is shown in Table 1 below.

gene name reference sequence
(NCBI Genbank No.) total number of exons number of amplicons Amplicon length (bp) ABL1 NM_007313 4/11 6 728 AKT1 NM_001014431 13/14 25 2,908 ALK NM_004304 29/29 64 7,093 AR NM_000044 8/8 29 3,293 BAP1 NM_004656 17/17 35 3,904 BRAF NM_004333 2/18 2 245 BRCA1 NM_007300 23/24 73 7,779 BRCA2 NM_000059 26/27 121 12,515 BTK NM_000061 1/19 One 127 CCND1 NM_053056 5/5 13 1,470 CD274 NM_014143 6/7 15 1,643 CDK4 NM_000075 7/8 13 1,469 CDK6 NM_001145306 7/8 16 1,775 CDKN2A NM_000077 2/3 8 935 DDR2 NM_006182 16/18 74 8,679 EGFR NM_005228 7/28 9 1,112 ERBB2 NM_004448 25/27 54 5,978 ERBB3 NM_001982 5/28 6 742 ERBB4 NM_005235 28/28 63 6,562 ESR1 NM_001122740 8/9 27 3,149 FGFR1 NM_001174067 18/19 39 4,364 FGFR2 NM_022970 17/18 45 4,742 FGFR3 NM_001163213 16/18 30 3,328 FGFR4 NM_213647 16/18 30 3,548 FLT3 NM_004119 4/24 4 485 GNA11 NM_002067 4/7 10 1,163 GNAQ NM_002072 4/7 7 813 HRAS NM_001130442 2/5 2 187 IDH1 NM_005896 1/10 One 123 IDH2 NM_002168 1/10 2 256 KIT NM_000222 9/21 10 1,217 KRAS NM_033360 3/6 3 336 MAP2K1 NM_002755 2/11 4 485 MET NM_001127500 5/21 7 851 MTOR NM_004958 4/58 5 613 MYCN NM_005378 2/3 8 824 NOTCH1 NM_017617 33/34 91 10,356 NRAS NM_002524 3/7 3 361 PDGFRA NM_006206 4/23 4 507 PIK3CA NM_006218 8/21 11 1,227 PPARG NM_015869 7/7 21 2,320 PTCH1 NM_000264 23/24 59 6,493 PTEN NM_000314 9/9 17 1,753 RAF1 NM_002880 4/17 4 500 RET NM_020975 7/20 57 6,638 ROS1 NM_002944 7/43 7 854 SMO NM_005631 5/12 5 637 STK11 NM_000455 9/10 16 1,924 TP53 NM_001276760 8/11 22 2,478 TSC1 NM_000368 21/23 49 5,521 TSC2 NM_000548 40/42 87 9,826

4. Performance verification of NGS panel

gene name reference sequence
(NCBI Genbank No.) total number of exons number of amplicons Amplicon length (bp) ALK NM_004304 29/29 64 7,093 AR NM_000044 8/8 29 3,293 BAP1 NM_004656 17/17 35 3,904 BRCA1 NM_007300 23/24 73 7,779 BRCA2 NM_000059 26/27 121 12,515 BTK NM_000061 1/19 One 127 CDK4 NM_000075 7/8 13 1,469 ERBB2 NM_004448 25/27 54 5,978 ERBB3 NM_001982 5/28 6 742 ESR1 NM_001122740 8/9 27 3,149 FGFR1 NM_001174067 18/19 39 4,364 FGFR2 NM_022970 17/18 45 4,742 FGFR3 NM_001163213 16/18 30 3,328 FGFR4 NM_213647 16/18 30 3,548 MYCN NM_005378 2/3 8 824 NOTCH1 NM_017617 33/34 91 10,356 PIK3CA NM_006218 8/21 11 1,227 PTCH1 NM_000264 23/24 59 6,493 RET NM_020975 7/20 57 6,638 STK11 NM_000455 9/10 16 1,924 TP53 NM_001276760 8/11 22 2,478 TSC1 NM_000368 21/23 49 5,521 TSC2 NM_000548 40/42 87 9,826

Multiplex 1 cfDNA Reference Standard set consists of 8 mutations in EGFR (mutations in L858R, E746-A750 del, T790M, and V769-D770insASV), KRAS (G12D mutation), NRAS (Q61K and A59T mutation), and PIK3A (E545K mutation) mutations. is constructed, configured, and has 0%, 0.1%, 1%, and 5% allele frequencies.

The results of analyzing the Multiplex 1 cfDNA Reference Standard set using the NGS panel prepared in Example 1.3 are shown in FIGS. 2a and 2b (in FIG. 2b, R2=0.9955).

As shown in FIGS. 2A and 2B , mutations in NRAS (Q61K, A59T), EGFR (E756-A750, T790M, L858R), and KRAS (G12D) were detected up to 1% allele frequency by the manufactured NGS panel. . Mutations in EGFR (V769-D770insASV) and PIK3CA (E545K) were detectable even at 0.1% allele frequency.

5. Validation of NGS Panels Using Blood Samples from Cancer Patients

The performance of the NGS panel prepared in Example 1.3 was verified using the blood of a gynecological cancer patient.

cfDNA and ctcDNA were isolated from the blood collected as described in Examples 1.1 and 1.2, and whole genome amplification was performed. PBMC-derived DNA was used as a normal control.

On the prepared patient-derived DNA samples (sample Nos. 1 to 20, respectively), NGS genetic analysis was performed using the NGS panel prepared in Example 1.3. As a result of NGS gene analysis, information related to cancer mutations or mutations specific to cfDNA and ctcDNA was collected by excluding PBMC analysis data from the entire analysis data. cfDNA-specific genetic mutations, ctcDNA-specific genetic mutations, and gene mutations common to cfDNA and ctcDNA detected in each patient-derived DNA sample were detected, and the results are shown in FIG. 3 .

As shown in FIG. 3 , 63 gene mutations in 23 genes were detected in the blood derived from 20 patients. Of the 63 gene mutations detected, 32 were cfDNA-specific and 22 were ctcDNA-specific. In addition, the nine gene mutations of BRCA2 (T7469C), ERBB2 (C3508G, C3149T), ESR1 (A1451G), FGFR4 (G1162A), PTCH1 (C3785T, C3943T), STK11 (C1062G), and TSC2 (T3524C) are cfDNA and ctcDNA was commonly detected in

Therefore, it was confirmed that, using the prepared NGS panel, cancer gene mutations can be detected with high sensitivity using a patient's blood sample, thereby being useful for cancer diagnosis, prognosis prediction, and monitoring.

<110> Clinomics Inc. <120> Gene panel for liquid biopsy anaylsis and method for personalized therapy using the same <130> P19-0901 <160> 51 <170> KoPatentIn 3.0 <210> 1 <211> 3450 <212> DNA <213> Artificial Sequence <220> <223> ABL1 <400> 1 atggggcagc agcctggaaa agtacttggg gaccaaagaa ggccaagctt gcctgccctg 60 cattttatca aaggagcagg gaagaaggaa tcatcgaggc atgggggtcc acactgcaat 120 gtttttgtgg aacatgaagc ccttcagcgg ccagtagcat ctgactttga gcctcagggt 180 ctgagtgaag ccgctcgttg gaactccaag gaaaaccttc tcgctggacc cagtgaaaat 240 gaccccaacc ttttcgttgc actgtatgat tttgtggcca gtggagataa cactctaagc 300 ataactaaag gtgaaaagct ccgggtctta ggctataatc acaatgggga atggtgtgaa 360 gcccaaacca aaaatggcca aggctgggtc ccaagcaact acatcacgcc agtcaacagt 420 ctggagaaac actcctggta ccatgggcct gtgtcccgca atgccgctga gtatctgctg 480 agcagcggga tcaatggcag cttcttggtg cgtgagagtg agagcagtcc tggccagagg 540 tccatctcgc tgagatacga agggagggtg taccattaca ggatcaacac tgcttctgat 600 ggcaagctct acgtctcctc cgagagccgc ttcaacaccc tggccgagtt ggttcatcat 660 cattcaacgg tggccgacgg gctcatcacc acgctccatt atccagcccc aaagcgcaac 720 aagcccactg tctatggtgt gtcccccaac tacgacaagt gggagatgga acgcacggac 780 atcaccatga agcacaagct gggcgggggc cagtacgggg aggtgtacga gggcgtgtgg 840 aagaaataca gcctgacggt ggccgtgaag accttgaagg aggacaccat ggaggtggaa 900 gagttcttga aagaagctgc agtcatgaaa gagatcaaac accctaacct ggtgcagctc 960 cttggggtct gcacccggga gcccccgttc tatatcatca ctgagttcat gacctacggg 1020 aacctcctgg actacctgag ggagtgcaac cggcaggagg tgaacgccgt ggtgctgctg 1080 tacatggcca ctcagatctc gtcagccatg gagtacctgg agaagaaaaa cttcatccac 1140 agagatcttg ctgcccgaaa ctgcctggta ggggagaacc acttggtgaa ggtagctgat 1200 tttggcctga gcaggttgat gacaggggac acctacacag cccatgctgg agccaagttc 1260 cccatcaaat ggactgcacc cgagagcctg gcctacaaca agttctccat caagtccgac 1320 gtctgggcat ttggagtatt gctttgggaa attgctacct atggcatgtc cccttacccg 1380 ggaattgacc tgtcccaggt gtatgagctg ctagagaagg actaccgcat ggagcgccca 1440 gaaggctgcc cagagaaggt ctatgaactc atgcgagcat gttggcagtg gaatccctct 1500 gaccggccct cctttgctga aatccaccaa gcctttgaaa caatgttcca ggaatccagt 1560 atctcagacg aagtggaaaa ggagctgggg aaacaaggcg tccgtggggc tgtgagtacc 1620 ttgctgcagg ccccagagct gcccaccaag acgaggacct ccaggagagc tgcagagcac 1680 agagacacca ctgacgtgcc tgagatgcct cactccaagg gccagggaga gagcgatcct 1740 ctggaccatg agcctgccgt gtctccattg ctccctcgaa aagagcgagg tccccccggag 1800 ggcggcctga atgaagatga gcgccttctc cccaaagaca aaaagaccaa cttgttcagc 1860 gccttgatca agaagaagaa gaagacagcc ccaacccctc ccaaacgcag cagctccttc 1920 cgggagatgg acggccagcc ggagcgcaga ggggccggcg aggaagaggg ccgagacatc 1980 agcaacgggg cactggcttt cacccccttg gacacagctg acccagccaa gtccccaaag 2040 cccagcaatg gggctggggt ccccaatgga gccctccggg agtccggggg ctcaggcttc 2100 cggtctcccc acctgtggaa gaagtccagc acgctgacca gcagccgcct agccaccggc 2160 gaggaggagg gcggtggcag ctccagcaag cgcttcctgc gctcttgctc cgcctcctgc 2220 gttccccatg gggccaagga cacggagtgg aggtcagtca cgctgcctcg ggacttgcag 2280 tccacgggaa gacagtttga ctcgtccaca tttggagggc acaaaagtga gaagccggct 2340 ctgcctcgga agagggcagg ggagaacagg tctgaccagg tgacccgagg cacagtaacg 2400 cctcccccca ggctggtgaa aaagaatgag gaagctgctg atgaggtctt caaagacatc 2460 atggagtcca gcccgggctc cagcccgccc aacctgactc caaaacccct ccggcggcag 2520 gtcaccgtgg cccctgcctc gggcctcccc cacaaggaag aagctggaaa gggcagtgcc 2580 ttagggaccc ctgctgcagc tgagccagtg accccccacca gcaaagcagg ctcaggtgca 2640 ccagggggca ccagcaaggg ccccgccgag gagtccagag tgaggaggca caagcactcc 2700 tctgagtcgc cagggaggga caaggggaaa ttgtccaggc tcaaacctgc cccgccgccc 2760 ccaccagcag cctctgcagg gaaggctgga ggaaagccct cgcagagccc gagccaggag 2820 gcggccgggg aggcagtcct gggcgcaaag acaaaagcca cgagtctggt tgatgctgtg 2880 aacagtgacg ctgccaagcc cagccagccg ggagagggcc tcaaaaagcc cgtgctcccg 2940 gccactccaa agccacagtc cgccaagccg tcggggaccc ccatcagccc agcccccgtt 3000 ccctccacgt tgccatcagc atcctcggcc ctggcagggg accagccgtc ttccaccgcc 3060 ttcatccctc tcatatcaac ccgagtgtct cttcggaaaa cccgccagcc tccagagcgg 3120 atcgccagcg gcgccatcac caagggcgtg gtcctggaca gcaccgaggc gctgtgcctc 3180 gccatctcta ggaactccga gcagatggcc agccacagcg cagtgctgga ggccggcaaa 3240 aacctctaca cgttctgcgt gagctatgtg gattccatcc agcaaatgag gaacaagttt 3300 gccttccgag aggccatcaa caaactggag aataatctcc gggagcttca gatctgcccg 3360 gcgacagcag gcagtggtcc agcggccact caggacttca gcaagctcct cagttcggtg 3420 aaggaaatca gtgacatagt gcagaggtag 3450 <210> 2 <211> 1443 <212> DNA <213> Artificial Sequence <220> <223> AKT1 <400> 2 atgagcgacg tggctattgt gaaggagggt tggctgcaca aacgagggga gtacatcaag 60 acctggcggc cacgctactt cctcctcaag aatgatggca ccttcattgg ctacaaggag 120 cggccgcagg atgtggacca acgtgaggct cccctcaaca acttctctgt ggcgcagtgc 180 cagctgatga agacggagcg gccccggccc aacaccttca tcatccgctg cctgcagtgg 240 accactgtca tcgaacgcac cttccatgtg gagactcctg aggagcggga ggagtggaca 300 accgccatcc agactgtggc tgacggcctc aagaagcagg aggaggagga gatggacttc 360 cggtcgggct cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag 420 cccaagcacc gcgtgaccat gaacgagttt gagtacctga agctgctggg caagggcact 480 ttcggcaagg tgatcctggt gaaggagaag gccacaggcc gctactacgc catgaagatc 540 ctcaagaagg aagtcatcgt ggccaaggac gaggtggccc acacactcac cgagaaccgc 600 gtcctgcaga actccaggca ccccttcctc acagccctga agtactcttt ccagacccac 660 gaccgcctct gctttgtcat gggatacgcc aacgggggcg agctgttctt ccacctgtcc 720 cgggagcgtg tgttctccga ggaccgggcc cgcttctatg gcgctgagat tgtgtcagcc 780 ctggactacc tgcactcgga gaagaacgtg gtgtaccggg acctcaagct ggagaacctc 840 atgctggaca aggacgggca cattaagatc acagacttcg ggctgtgcaa ggaggggatc 900 aaggacggtg ccaccatgaa gaccttttgc ggcacacctg agtacctggc ccccgaggtg 960 ctggaggaca atgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac 1020 gagatgatgt gcggtcgcct gcccttctac aaccaggacc atgagaagct ttttgagctc 1080 atcctcatgg aggagatccg cttcccgcgc acgcttggtc ccgaggccaa gtccttgctt 1140 tcagggctgc tcaagaagga ccccaagcag aggcttggcg ggggctccga ggacgccaag 1200 gagatcatgc agcatcgctt ctttgccggt atcgtgtggc agcacgtgta cgagaagaag 1260 ctcagcccac ccttcaagcc ccaggtcacg tcggagactg acaccaggta ttttgatgag 1320 gagttcacgg cccagatgat caccatcaca ccacctgacc aagatgacag catggagtgt 1380 gtggacagcg agcgcaggcc ccacttcccc cagttctcct actcggccag cggcacggcc 1440 tga 1443 <210> 3 <211> 6240 <212> DNA <213> Artificial Sequence <220> <223> ALK <400> 3 agatgcgatc cagcggctct gggggcggca gcggtggtag cagctggtac ctcccgccgc 60 ctctgttcgg agggtcgcgg ggcaccgagg tgctttccgg ccgccctctg gtcggccacc 120 caaagccgcg ggcgctgatg atgggtgagg agggggcggc aagatttcgg gcgcccctgc 180 cctgaacgcc ctcagctgct gccgccgggg ccgctccagt gcctgcgaac tctgaggagc 240 cgaggcgccg gtgagagcaa ggacgctgca aacttgcgca gcgcgggggc tgggattcac 300 gcccagaagt tcagcaggca gacagtccga agccttcccg cagcggagag atagcttgag 360 ggtgcgcaag acggcagcct ccgccctcgg ttcccgccca gaccgggcag aagagcttgg 420 aggagccaaa aggaacgcaa aaggcggcca ggacagcgtg cagcagctgg gagccgccgt 480 tctcagcctt aaaagttgca gagattggag gctgccccga gaggggacag accccagctc 540 cgactgcggg gggcaggaga ggacggtacc caactgccac ctcccttcaa ccatagtagt 600 tcctctgtac cgagcgcagc gagctacaga cggggggcgcg gcactcggcg cggagagcgg 660 gaggctcaag gtcccagcca gtgagcccag tgtgcttgag tgtctctgga ctcgcccctg 720 agcttccagg tctgtttcat ttagactcct gctcgcctcc gtgcagttgg gggaaagcaa 780 gagacttgcg cgcacgcaca gtcctctgga gatcaggtgg aaggagccgc tgggtaccaa 840 ggactgttca gagcctcttc ccatctcggg gagagcgaag ggtgaggctg ggcccggaga 900 gcagtgtaaa cggcctcctc cggcgggatg ggagccatcg ggctcctgtg gctcctgccg 960 ctgctgcttt ccacggcagc tgtgggctcc gggatgggga ccggccagcg cgcgggctcc 1020 ccagctgcgg ggccgccgct gcagccccgg gagccactca gctactcgcg cctgcagagg 1080 aagagtctgg cagttgactt cgtggtgccc tcgctcttcc gtgtctacgc ccgggaccta 1140 ctgctgccac catcctcctc ggagctgaag gctggcaggc ccgaggcccg cggctcgcta 1200 gctctggact gcgccccgct gctcaggttg ctggggccgg cgccgggggt ctcctggacc 1260 gccggttcac cagccccggc agaggcccgg acgctgtcca gggtgctgaa gggcggctcc 1320 gtgcgcaagc tccggcgtgc caagcagttg gtgctggagc tgggcgagga ggcgatcttg 1380 gagggttgcg tcgggccccc cggggaggcg gctgtggggc tgctccagtt caatctcagc 1440 gagctgttca gttggtggat tcgccaaggc gaagggcgac tgaggatccg cctgatgccc 1500 gagaagaagg cgtcggaagt gggcagagag ggaaggctgt ccgcggcaat tcgcgcctcc 1560 cagccccgcc ttctcttcca gatcttcggg actggtcata gctccttgga atcaccaaca 1620 aacatgcctt ctccttctcc tgattatttt acatggaatc tcacctggat aatgaaagac 1680 tccttccctt tcctgtctca tcgcagccga tatggtctgg agtgcagctt tgacttcccc 1740 tgtgagctgg agtattcccc tccactgcat gacctcagga accagagctg gtcctggcgc 1800 cgcatcccct ccgaggaggc ctcccagatg gacttgctgg atgggcctgg ggcagagcgt 1860 tctaaggaga tgcccagagg ctcctttctc cttctcaaca cctcagctga ctccaagcac 1920 accatcctga gtccgtggat gaggagcagc agtgagcact gcacactggc cgtctcggtg 1980 cacaggcacc tgcagccctc tggaaggtac attgcccagc tgctgcccca caacgaggct 2040 gcaagagaga tcctcctgat gcccactcca gggaagcatg gttggacagt gctccaggga 2100 agaatcgggc gtccagacaa cccatttcga gtggccctgg aatacatctc cagtggaaac 2160 cgcagcttgt ctgcagtgga cttctttgcc ctgaagaact gcagtgaagg aacatcccca 2220 ggctccaaga tggccctgca gagctccttc acttgttgga atgggacagt cctccagctt 2280 gggcaggcct gtgacttcca ccaggactgt gcccagggag aagatgagag ccagatgtgc 2340 cggaaactgc ctgtgggttt ttactgcaac tttgaagatg gcttctgtgg ctggacccaa 2400 ggcacactgt caccccacac tcctcaatgg caggtcagga ccctaaagga tgcccggttc 2460 caggaccacc aagaccatgc tctattgctc agtaccactg atgtccccgc ttctgaaagt 2520 gctacagtga ccagtgctac gtttcctgca ccgatcaaga gctctccatg tgagctccga 2580 atgtcctggc tcattcgtgg agtcttgagg ggaaacgtgt ccttggtgct agtggagaac 2640 aaaaccggga aggagcaagg caggatggtc tggcatgtcg ccgcctatga aggcttgagc 2700 ctgtggcagt ggatggtgtt gcctctcctc gatgtgtctg acaggttctg gctgcagatg 2760 gtcgcatggt ggggacaagg atccagagcc atcgtggctt ttgacaatat ctccatcagc 2820 ctggactgct acctcaccat tagcggagag gacaagatcc tgcagaatac agcacccaaa 2880 tcaagaaacc tgtttgagag aaacccaaac aaggagctga aacccgggga aaattcacca 2940 agacagaccc ccatctttga ccctacagtt cattggctgt tcaccacatg tggggccagc 3000 gggccccatg gccccaccca ggcacagtgc aacaacgcct accagaactc caacctgagc 3060 gtggaggtgg ggagcgaggg ccccctgaaa ggcatccaga tctggaaggt gccagccacc 3120 gacacctaca gcatctcggg ctacggagct gctggcggga aaggcgggaa gaacaccatg 3180 atgcggtccc acggcgtgtc tgtgctgggc atcttcaacc tggagaagga tgacatgctg 3240 tacatcctgg ttgggcagca gggagaggac gcctgcccca gtacaaacca gttaatccag 3300 aaagtctgca ttggagagaa caatgtgata gaagaagaaa tccgtgtgaa cagaagcgtg 3360 catgagtggg caggaggcgg aggaggaggg ggtggagcca cctacgtatt taagatgaag 3420 gatggagtgc cggtgcccct gatcattgca gccggaggtg gtggcagggc ctacggggcc 3480 aagacagaca cgttccaccc agagagactg gagaataact cctcggttct agggctaaac 3540 ggcaattccg gagccgcagg tggtggaggt ggctggaatg ataacacttc cttgctctgg 3600 gccggaaaat ctttgcagga gggtgccacc ggaggacatt cctgccccca ggccatgaag 3660 aagtgggggt gggagacaag agggggtttc ggagggggtg gaggggggtg ctcctcaggt 3720 ggaggaggcg gaggatatat aggcggcaat gcagcctcaa acaatgaccc cgaaatggat 3780 ggggaagatg gggtttcctt catcagtcca ctgggcatcc tgtacacccc agctttaaaa 3840 gtgatggaag gccacgggga agtgaatatt aagcattatc taaactgcag tcactgtgag 3900 gtagacgaat gtcacatgga ccctgaaagc cacaaggtca tctgcttctg tgaccacggg 3960 acggtgctgg ctgaggatgg cgtctcctgc attgtgtcac ccaccccgga gccacacctg 4020 ccactctcgc tgatcctctc tgtggtgacc tctgccctcg tggccgccct ggtcctggct 4080 ttctccggca tcatgattgt gtaccgccgg aagcaccagg agctgcaagc catgcagatg 4140 gagctgcaga gccctgagta caagctgagc aagctccgca cctcgaccat catgaccgac 4200 tacaacccca actactgctt tgctggcaag acctcctcca tcagtgacct gaaggaggtg 4260 ccgcggaaaa acatcaccct cattcggggt ctgggccatg gcgcctttgg ggaggtgtat 4320 gaaggccagg tgtccggaat gcccaacgac ccaagccccc tgcaagtggc tgtgaagacg 4380 ctgcctgaag tgtgctctga acaggacgaa ctggatttcc tcatggaagc cctgatcatc 4440 agcaaattca accaccagaa cattgttcgc tgcattgggg tgagcctgca atccctgccc 4500 cggttcatcc tgctggagct catggcgggg ggagacctca agtccttcct ccgagagacc 4560 cgccctcgcc cgagccagcc ctcctccctg gccatgctgg accttctgca cgtggctcgg 4620 gacattgcct gtggctgtca gtatttggag gaaaaccact tcatccaccg agacattgct 4680 gccagaaact gcctcttgac ctgtccaggc cctggaagag tggccaagat tggagacttc 4740 gggatggccc gagacatcta cagggcgagc tactatagaa agggaggctg tgccatgctg 4800 ccagttaagt ggatgccccc agaggccttc atggaaggaa tattcacttc taaaacagac 4860 acatggtcct ttggagtgct gctatgggaa atcttttctc ttggatatat gccatacccc 4920 agcaaaagca accaggaagt tctggagttt gtcaccagtg gaggccggat ggacccaccc 4980 aagaactgcc ctgggcctgt ataccggata atgactcagt gctggcaaca tcagcctgaa 5040 gacaggccca actttgccat cattttggag aggattgaat actgcaccca ggacccggat 5100 gtaatcaaca ccgctttgcc gatagaatat ggtccacttg tggaagagga agagaaagtg 5160 cctgtgaggc ccaaggaccc tgagggggtt cctcctctcc tggtctctca acaggcaaaa 5220 cgggaggagg agcgcagccc agctgcccca ccacctctgc ctaccacctc ctctggcaag 5280 gctgcaaaga aacccacagc tgcagagatc tctgttcgag tccctagagg gccggccgtg 5340 gaagggggac acgtgaatat ggcattctct cagtccaacc ctccttcgga gttgcacaag 5400 gtccacggat ccagaaacaa gccccaccagc ttgtggaacc caacgtacgg ctcctggttt 5460 acagagaaac ccaccaaaaa gaataatcct atagcaaaga aggagccaca cgacaggggt 5520 aacctggggc tggagggaag ctgtactgtc ccacctaacg ttgcaactgg gagacttccg 5580 ggggcctcac tgctcctaga gccctcttcg ctgactgcca atatgaagga ggtacctctg 5640 ttcaggctac gtcacttccc ttgtgggaat gtcaattacg gctaccagca acagggcttg 5700 cccttagaag ccgctactgc ccctggagct ggtcattacg aggataccat tctgaaaagc 5760 aagaatagca tgaaccagcc tgggccctga gctcggtcgc acactcactt ctcttccttg 5820 ggatccctaa gaccgtggag gagagagagg caatggctcc ttcacaaacc agagaccaaa 5880 tgtcacgttt tgttttgtgc caacctattt tgaagtacca ccaaaaaagc tgtattttga 5940 aaatgcttta gaaaggtttt gagcatgggt tcatcctatt ctttcgaaag aagaaaatat 6000 cataaaaatg agtgataaat acaaggccca gatgtggttg cataaggttt ttatgcatgt 6060 ttgttgtata cttccttatg cttctttcaa attgtgtgtg ctctgcttca atgtagtcag 6120 aattagctgc ttctatgttt catagttggg gtcatagatg tttccttgcc ttgttgatgt 6180 ggacatgagc catttgaggg gagagggaac ggaaataaag gagttatttg taatgactaa 6240 6240 <210> 4 <211> 1947 <212> DNA <213> Artificial Sequence <220> <223> AR <400> 4 atggaagtgc agttagggct gggaagggtc taccctcggc cgccgtccaa gacctaccga 60 ggagctttcc agaatctgtt ccagagcgtg cgcgaagtga tccagaaccc gggccccagg 120 cacccagagg ccgcgagcgc agcacctccc ggcgccagtt tgctgctgct gcagcagcag 180 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcaa 240 gagactagcc ccaggcagca gcagcagcag cagggtgagg atggttctcc ccaagcccat 300 cgtagaggcc ccacaggcta cctggtcctg gatgaggaac agcaaccttc acagccgcag 360 tcggccctgg agtgccaccc cgagagaggt tgcgtcccag agcctggagc cgccgtggcc 420 gccagcaagg ggctgccgca gcagctgcca gcacctccgg acgaggatga ctcagctgcc 480 ccatccacgt tgtccctgct gggccccact ttccccggct taagcagctg ctccgctgac 540 cttaaagaca tcctgagcga ggccagcacc atgcaactcc ttcagcaaca gcagcaggaa 600 gcagtatccg aaggcagcag cagcgggaga gcgagggagg cctcgggggc tcccacttcc 660 tccaaggaca attacttagg gggcacttcg accatttctg acaacgccaa ggagttgtgt 720 aaggcagtgt cggtgtccat gggcctgggt gtggaggcgt tggagcatct gagtccaggg 780 gaacagcttc ggggggattg catgtacgcc ccacttttgg gagttccacc cgctgtgcgt 840 cccactcctt gtgccccatt ggccgaatgc aaaggttctc tgctagacga cagcgcaggc 900 aagagcactg aagatactgc tgagtattcc cctttcaagg gaggttacac caaagggcta 960 gaaggcgaga gcctaggctg ctctggcagc gctgcagcag ggagctccgg gacacttgaa 1020 ctgccgtcta ccctgtctct ctacaagtcc ggagcactgg acgaggcagc tgcgtaccag 1080 agtcgcgact actacaactt tccactggct ctggccggac cgccgccccc tccgccgcct 1140 ccccatcccc acgctcgcat caagctggag aacccgctgg actacggcag cgcctgggcg 1200 gctgcggcgg cgcagtgccg ctatggggac ctggcgagcc tgcatggcgc gggtgcagcg 1260 ggacccggtt ctgggtcacc ctcagccgcc gcttcctcat cctggcacac tctcttcaca 1320 gccgaagaag gccagttgta tggaccgtgt ggtggtggtg ggggtggtgg cggcggcggc 1380 ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg aggcgggagc tgtagccccc 1440 tacggctaca ctcggccccc tcaggggctg gcgggccagg aaagcgactt caccgcacct 1500 gatgtgtggt accctggcgg catggtgagc agagtgccct atcccagtcc cacttgtgtc 1560 aaaagcgaaa tgggcccctg gatggatagc tactccggac cttacgggga catgcgtttg 1620 gagactgcca gggaccatgt tttgcccatt gactattact ttccacccca gaagacctgc 1680 ctgatctgtg gagatgaagc ttctgggtgt cactatggag ctctcacatg tggaagctgc 1740 aaggtcttct tcaaaagagc cgctgaaggg aaacagaagt acctgtgcgc cagcagaaat 1800 gattgcacta ttgataaatt ccgaaggaaa aattgtccat cttgtcgtct tcggaaatgt 1860 tatgaagcag ggatgactct gggagcagct gttgttgttt ctgaaagaat cttgagggtg 1920 tttggagtct cagaatggct tccttaa 1947 <210> 5 <211> 2190 <212> DNA <213> Artificial Sequence <220> <223> BAP1 <400> 5 atgaataagg gctggctgga gctggagagc gacccaggcc tcttcaccct gctcgtggaa 60 gatttcggtg tcaagggggt gcaagtggag gagatctacg accttcagag caaatgtcag 120 ggccctgtat atggatttat cttcctgttc aaatggatcg aagagcgccg gtccccggcga 180 aaggtctcta ccttggtgga tgatacgtcc gtgattgatg atgatattgt gaataacatg 240 ttctttgccc accagctgat acccaactct tgtgcaactc atgccttgct gagcgtgctc 300 ctgaactgca gcagcgtgga cctgggaccc accctgagtc gcatgaagga cttcaccaag 360 ggtttcagcc ctgagagcaa aggatatgcg attggcaatg ccccggagtt ggccaaggcc 420 cataatagcc atgccaggcc cgagccacgc cacctccctg agaagcagaa tggccttagt 480 gcagtgcgga ccatggaggc gttccacttt gtcagctatg tgcctatcac aggccggctc 540 tttgagctgg atgggctgaa ggtctacccc attgaccatg ggccctgggg ggaggacgag 600 gagtggacag acaaggcccg gcgggtcatc atggagcgta tcggcctcgc cactgcaggg 660 gagccctacc acgacatccg cttcaacctg atggcagtgg tgcccgaccg caggatcaag 720 tatgaggcca ggctgcatgt gctgaaggtg aaccgtcaga cagtactaga ggctctgcag 780 cagctgataa gagtaacaca gccagagctg attcagaccc acaagtctca agagtcacag 840 ctgcctgagg agtccaagtc agccagcaac aagtccccgc tggtgctgga agcaaacagg 900 gcccctgcag cctctgaggg caaccacaca gatggtgcag aggaggcggc tggttcatgc 960 gcacaagccc catcccacag ccctcccaac aaacccaagc tagtggtgaa gcctccaggc 1020 agcagcctca atggggttca ccccaacccc actcccattg tccagcggct gccggccttt 1080 ctagacaatc acaattatgc caagtccccc atgcaggagg aagaagacct ggcggcaggt 1140 gtgggccgca gccgagttcc agtccgccca ccccagcagt actcagatga tgaggatgac 1200 tatgaggatg acgaggagga tgacgtgcag aacaccaact ctgcccttag gtataagggg 1260 aagggaacag ggaagccagg ggcattgagc ggttctgctg atgggcaact gtcagtgctg 1320 cagcccaaca ccatcaacgt cttggctgag aagctcaaag agtcccagaa ggacctctca 1380 attcctctgt ccatcaagac tagcagcggg gctgggagtc cggctgtggc agtgcccaca 1440 cactcgcagc cctcacccac ccccagcaat gagagtacag acacggcctc tgagatcggc 1500 agtgctttca actcgccact gcgctcgcct atccgctcag ccaacccgac gcggccctcc 1560 agccctgtca cctcccacat ctccaaggtg ctttttggag aggatgacag cctgctgcgt 1620 gttgactgca tacgctacaa ccgtgctgtc cgtgatctgg gtcctgtcat cagcacaggc 1680 ctgctgcacc tggctgagga tggggtgctg agtcccctgg cgctgacaga gggtgggaag 1740 ggttcctcgc cctccatcag accaatccaa ggcagccagg ggtccagcag cccagtggag 1800 aaggaggtcg tggaagccac ggacagcaga gagaagacgg ggatggtgag gcctggcgag 1860 cccttgagtg gggagaaata ctcacccaag gagctgctgg cactgctgaa gtgtgtggag 1920 gctgagattg caaactatga ggcgtgcctc aaggaggagg tagagaagag gaagaagttc 1980 aagattgatg accagagaag gacccacaac tacgatgagt tcatctgcac ctttatctcc 2040 atgctggctc aggaaggcat gctggccaac ctagtggagc agaacatctc cgtgcggcgg 2100 cgccaagggg tcagcatcgg ccggctccac aagcagcgga agcctgaccg gcggaaacgc 2160 tctcgcccct acaaggccaa gcgccagtga 2190 <210> 6 <211> 2304 <212> DNA <213> Artificial Sequence <220> <223> BRAF <400> 6 atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc tctgttcaac 60 ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc ggctgcggac 120 cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac acaggaacat 180 atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat atatctggag 240 gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca acagttattg 300 gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat ggataccgtt 360 acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt ttttcaaaat 420 cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt tagagtcttc 480 ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt ccgagacagt 540 ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt ttacagaatt 600 caggatggag agaagaaacc aattggttgg gacactgata tttcctggct tactggagaa 660 gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt tgtacgaaaa 720 acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca gggtttccgc 780 tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc actgatgtgt 840 gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca ccacccaata 900 ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc cccttccgca 960 cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc aaaatccatt 1020 ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt tgggcaacga 1080 gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt caatattgat 1140 gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg tttgtctgct 1200 accccccctg cctcattacc tggctcacta actaacgtga aagccttaca gaaatctcca 1260 ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg aatgaaaaca 1320 cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat tacagtggga 1380 caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca tggtgatgtg 1440 gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc cttcaaaaat 1500 gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat gggctattcc 1560 acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt gtatcaccat 1620 ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc accacagact 1680 gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct caagagtaat 1740 aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct agctacagtg 1800 aaatctcgat ggagtgggtc ccatcagttt gaacagttgt ctggatccat tttgtggatg 1860 gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc agatgtatat 1920 gcatttggaa ttgttctgta tgaattgatg actggacagt taccttattc aaacatcaac 1980 aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga tctcagtaag 2040 gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct caaaaagaaa 2100 agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct ggcccgctca 2160 ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg tttccaaaca 2220 gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc agggggatat 2280 ggagaatttg cagccttcaa gtag 2304 <210> 7 <211> 2280 <212> DNA <213> Artificial Sequence <220> <223> BRCA1 <400> 7 atggatttat ctgctcttcg cgttgaagaa gtacaaaatg tcattaatgc tatgcagaaa 60 atcttagagt gtcccatctg tctggagttg atcaaggaac ctgtctccac aaagtgtgac 120 cacatatttt gcaaattttg catgctgaaa cttctcaacc agaagaaagg gccttcacag 180 tgtcctttat gtaagaatga tataaccaaa aggagcctac aagaaagtac gagatttagt 240 caacttgttg aagagctatt gaaaatcatt tgtgcttttc agcttgacac aggtttggag 300 tatgcaaaca gctataattt tgcaaaaaag gaaaataact ctcctgaaca tctaaaagat 360 gaagtttcta tcatccaaag tatgggctac agaaaccgtg ccaaaagact tctacagagt 420 gaacccgaaa atccttcctt gcaggaaacc agtctcagtg tccaactctc taaccttgga 480 actgtgagaa ctctgaggac aaagcagcgg atacaacctc aaaagacgtc tgtctacatt 540 gaattgggat ctgattcttc tgaagatacc gttaataagg caacttattg cagtgtggga 600 gatcaagaat tgttacaaat cacccctcaa ggaaccaggg atgaaatcag tttggattct 660 gcaaaaaagg ctgcttgtga attttctgag acggatgtaa caaatactga acatcatcaa 720 cccagtaata atgatttgaa caccactgag aagcgtgcag ctgagaggca tccagaaaag 780 tatcagggtg aagcagcatc tgggtgtgag agtgaaacaa gcgtctctga agactgctca 840 gggctatcct ctcagagtga cattttaacc actcagcaga gggataccat gcaacataac 900 ctgataaagc tccagcagga aatggctgaa ctagaagctg tgttagaaca gcatgggagc 960 cagccttcta acagctaccc ttccatcata agtgactctt ctgcccttga ggacctgcga 1020 aatccagaac aaagcacatc agaaaaagta ttaacttcac agaaaagtag tgaataccct 1080 ataagccaga atccagaagg cctttctgct gacaagtttg aggtgtctgc agatagttct 1140 accagtaaaa ataaagaacc aggagtggaa aggtcatccc cttctaaatg cccatcatta 1200 gatgataggt ggtacatgca cagttgctct gggagtcttc agaatagaaa ctacccatct 1260 caagaggagc tcattaaggt tgttgatgtg gaggagcaac agctggaaga gtctgggcca 1320 cacgatttga cggaaacatc ttacttgcca aggcaagatc tagagggaac cccttacctg 1380 gaatctggaa tcagcctctt ctctgatgac cctgaatctg atccttctga agacagagcc 1440 ccagagtcag ctcgtgttgg caacatacca tcttcaacct ctgcattgaa agttccccaa 1500 ttgaaagttg cagaatctgc ccagagtcca gctgctgctc atactactga tactgctggg 1560 tataatgcaa tggaagaaag tgtgagcagg gagaagccag aattgacagc ttcaacagaa 1620 agggtcaaca aaagaatgtc catggtggtg tctggcctga ccccagaaga atttatgctc 1680 gtgtacaagt ttgccagaaa acaccacatc actttaacta atctaattac tgaagagact 1740 actcatgttg ttatgaaaac agatgctgag tttgtgtgtg aacggacact gaaatatttt 1800 ctaggaattg cgggaggaaa atgggtagtt agctatttct gggtgaccca gtctattaaa 1860 gaaagaaaaa tgctgaatga gcatgatttt gaagtcagag gagatgtggt caatggaaga 1920 aaccaccaag gtccaaagcg agcaagagaa tcccaggaca gaaagatctt cagggggcta 1980 gaaatctgtt gctatgggcc cttcaccaac atgcccacag atcaactgga atggatggta 2040 cagctgtgtg gtgcttctgt ggtgaaggag ctttcatcat tcacccttgg cacaggtgtc 2100 cacccaattg tggttgtgca gccagatgcc tggacagagg acaatggctt ccatgcaatt 2160 gggcagatgt gtgaggcacc tgtggtgacc cgagagtggg tgttggacag tgtagcactc 2220 taccagtgcc aggagctgga cacctacctg ataccccaga tcccccacag ccactactga 2280 2280 <210> 8 <211> 10257 <212> DNA <213> Artificial Sequence <220> <223> BRCA2 <400> 8 atgcctattg gatccaaaga gaggccaaca ttttttgaaa tttttaagac acgctgcaac 60 aaagcagatt taggaccaat aagtcttaat tggtttgaag aactttcttc agaagctcca 120 ccctataatt ctgaacctgc agaagaatct gaacataaaa acaacaatta cgaaccaaac 180 ctatttaaaa ctccacaaag gaaaccatct tataatcagc tggcttcaac tccaataata 240 ttcaaagagc aagggctgac tctgccgctg taccaatctc ctgtaaaaga attagataaa 300 ttcaaattag acttaggaag gaatgttccc aatagtagac ataaaagtct tcgcacagtg 360 aaaactaaaa tggatcaagc agatgatgtt tcctgtccac ttctaaattc ttgtcttagt 420 gaaagtcctg ttgttctaca atgtacacat gtaacaccac aaagagataa gtcagtggta 480 tgtgggagtt tgtttcatac accaaagttt gtgaagggtc gtcagacacc aaaacatatt 540 tctgaaagtc taggagctga ggtggatcct gatatgtctt ggtcaagttc tttagctaca 600 ccacccaccc ttagttctac tgtgctcata gtcagaaatg aagaagcatc tgaaactgta 660 tttcctcatg atactactgc taatgtgaaa agctattttt ccaatcatga tgaaagtctg 720 aagaaaaatg atagatttat cgcttctgtg acagacagtg aaaacacaaa tcaaagagaa 780 gctgcaagtc atggatttgg aaaaacatca gggaattcat ttaaagtaaa tagctgcaaa 840 gaccacattg gaaagtcaat gccaaatgtc ctagaagatg aagtatatga aacagttgta 900 gatacctctg aagaagatag tttttcatta tgtttttcta aatgtagaac aaaaaatcta 960 caaaaagtaa gaactagcaa gactaggaaa aaaattttcc atgaagcaaa cgctgatgaa 1020 tgtgaaaaat ctaaaaacca agtgaaagaa aaatactcat ttgtatctga agtggaacca 1080 aatgatactg atccattaga ttcaaatgta gcaaatcaga agccctttga gagtggaagt 1140 gacaaaatct ccaaggaagt tgtaccgtct ttggcctgtg aatggtctca actaaccctt 1200 tcaggtctaa atggagccca gatggagaaa atacccctat tgcatatttc ttcatgtgac 1260 caaaatattt cagaaaaaga cctattagac acagagaaca aaagaaagaa agattttctt 1320 acttcagaga attctttgcc acgtatttct agcctaccaa aatcagagaa gccattaaat 1380 gaggaaacag tggtaaataa gagagatgaa gagcagcatc ttgaatctca tacagactgc 1440 attcttgcag taaagcaggc aatatctgga acttctccag tggcttcttc atttcagggt 1500 atcaaaaagt ctatattcag aataagagaa tcacctaaag agactttcaa tgcaagtttt 1560 tcaggtcata tgactgatcc aaactttaaa aaagaaactg aagcctctga aagtggactg 1620 gaaatacata ctgtttgctc acagaaggag gactccttat gtccaaattt aattgataat 1680 ggaagctggc cagccaccac cacacagaat tctgtagctt tgaagaatgc aggtttaata 1740 tccactttga aaaagaaaac aaataagttt atttatgcta tacatgatga aacatcttat 1800 aaaggaaaaa aaataccgaa agaccaaaaa tcagaactaa ttaactgttc agcccagttt 1860 gaagcaaatg cttttgaagc accacttaca tttgcaaatg ctgattcagg tttattgcat 1920 tcttctgtga aaagaagctg ttcacagaat gattctgaag aaccaacttt gtccttaact 1980 agctcttttg ggacaattct gaggaaatgt tctagaaatg aaacatgttc taataataca 2040 gtaatctctc aggatcttga ttataaagaa gcaaaatgta ataaggaaaa actacagtta 2100 tttattaccc cagaagctga ttctctgtca tgcctgcagg aaggacagtg tgaaaatgat 2160 ccaaaaagca aaaaagtttc agatataaaa gaagaggtct tggctgcagc atgtcaccca 2220 gtacaacatt caaaagtgga atacagtgat actgactttc aatcccagaa aagtctttta 2280 tatgatcatg aaaatgccag cactcttatt ttaactccta cttccaagga tgttctgtca 2340 aacctagtca tgatttctag aggcaaagaa tcatacaaaa tgtcagacaa gctcaaaggt 2400 aacaattatg aatctgatgt tgaattaacc aaaaatattc ccatggaaaa gaatcaagat 2460 gtatgtgctt taaatgaaaa ttataaaaac gttgagctgt tgccacctga aaaatacatg 2520 agagtagcat caccttcaag aaaggtacaa ttcaaccaaa acacaaatct aagagtaatc 2580 caaaaaaatc aagaagaaac tacttcaatt tcaaaaataa ctgtcaatcc agactctgaa 2640 gaacttttct cagacaatga gaataatttt gtcttccaag tagctaatga aaggaataat 2700 cttgctttag gaaatactaa ggaacttcat gaaacagact tgacttgtgt aaacgaaccc 2760 attttcaaga actctaccat ggttttatat ggagacacag gtgataaaca agcaacccaa 2820 gtgtcaatta aaaaagattt ggtttatgtt cttgcagagg agaacaaaaa tagtgtaaag 2880 cagcatataa aaatgactct aggtcaagat ttaaaatcgg acatctcctt gaatatagat 2940 aaaataccag aaaaaaataa tgattacatg aacaaatggg caggactctt aggtccaatt 3000 tcaaatcaca gttttggagg tagcttcaga acagcttcaa ataaggaaat caagctctct 3060 gaacataaca ttaagaagag caaaatgttc ttcaaagata ttgaagaaca atatcctact 3120 agtttagctt gtgttgaaat tgtaaatacc ttggcattag ataatcaaaa gaaactgagc 3180 aagcctcagt caattaatac tgtatctgca catttacaga gtagtgtagt tgtttctgat 3240 tgtaaaaata gtcatataac ccctcagatg ttattttcca agcaggattt taattcaaac 3300 cataatttaa cacctagcca aaaggcagaa attacagaac tttctactat attagaagaa 3360 tcaggaagtc agtttgaatt tactcagttt agaaaaccaa gctacatatt gcagaagagt 3420 acatttgaag tgcctgaaaa ccagatgact atcttaaaga ccacttctga ggaatgcaga 3480 gatgctgatc ttcatgtcat aatgaatgcc ccatcgattg gtcaggtaga cagcagcaag 3540 caatttgaag gtacagttga aattaaacgg aagtttgctg gcctgttgaa aaatgactgt 3600 aacaaaagtg cttctggtta tttaacagat gaaaatgaag tggggtttag gggcttttat 3660 tctgctcatg gcacaaaact gaatgtttct actgaagctc tgcaaaaagc tgtgaaactg 3720 tttagtgata ttgagaatat tagtgaggaa acttctgcag aggtacatcc aataagttta 3780 tcttcaagta aatgtcatga ttctgttgtt tcaatgttta agatagaaaa tcataatgat 3840 aaaactgtaa gtgaaaaaaa taataaatgc caactgatat tacaaaataa tattgaaatg 3900 actactggca cttttgttga agaaattact gaaaattaca agagaaatac tgaaaatgaa 3960 gataacaaat atactgctgc cagtagaaat tctcataact tagaatttga tggcagtgat 4020 tcaagtaaaa atgatactgt ttgtattcat aaagatgaaa cggacttgct atttactgat 4080 cagcacaaca tatgtcttaa attatctggc cagtttatga aggagggaaa cactcagatt 4140 aaagaagatt tgtcagattt aacttttttg gaagttgcga aagctcaaga agcatgtcat 4200 ggtaatactt caaataaaga acagttaact gctactaaaa cggagcaaaa tataaaagat 4260 tttgagactt ctgatacatt ttttcagact gcaagtggga aaaatattag tgtcgccaaa 4320 gagtcattta ataaaattgt aaatttcttt gatcagaaac cagaagaatt gcataacttt 4380 tccttaaatt ctgaattaca ttctgacata agaaagaaca aaatggacat tctaagttat 4440 gaggaaacag acatagttaa acacaaaata ctgaaagaaa gtgtcccagt tggtactgga 4500 aatcaactag tgaccttcca gggacaaccc gaacgtgatg aaaagatcaa agaacctact 4560 ctattgggtt ttcatacagc tagcgggaaa aaagttaaaa ttgcaaagga atctttggac 4620 aaagtgaaaa acctttttga tgaaaaagag caaggtacta gtgaaatcac cagttttagc 4680 catcaatggg caaagaccct aaagtacaga gaggcctgta aagaccttga attagcatgt 4740 gagaccattg agatcacagc tgccccaaag tgtaaagaaa tgcagaattc tctcaataat 4800 gataaaaacc ttgtttctat tgagactgtg gtgccaccta agctcttaag tgataattta 4860 tgtagacaaa ctgaaaatct caaaacatca aaaagtatct ttttgaaagt taaagtacat 4920 gaaaatgtag aaaaagaaac agcaaaaagt cctgcaactt gttacacaaa tcagtcccct 4980 tattcagtca ttgaaaattc agccttagct ttttacacaa gttgtagtag aaaaacttct 5040 gtgagtcaga cttcattact tgaagcaaaa aaatggctta gagaaggaat atttgatggt 5100 caaccagaaa gaataaatac tgcagattat gtaggaaatt atttgtatga aaataattca 5160 aacagtacta tagctgaaaa tgacaaaaat catctctccg aaaaacaaga tacttattta 5220 agtaacagta gcatgtctaa cagctattcc taccattctg atgaggtata taatgattca 5280 ggatatctct caaaaaataa acttgattct ggtattgagc cagtattgaa gaatgttgaa 5340 gatcaaaaaa acactagttt ttccaaagta atatccaatg taaaagatgc aaatgcatac 5400 ccacaaactg taaatgaaga tatttgcgtt gaggaacttg tgactagctc ttcaccctgc 5460 aaaaataaaa atgcagccat taaattgtcc atatctaata gtaataattt tgaggtaggg 5520 ccacctgcat ttaggatagc cagtggtaaa atcgtttgtg tttcacatga aacaattaaa 5580 aaagtgaaag acatatttac agacagtttc agtaaagtaa ttaaggaaaa caacgagaat 5640 aaatcaaaaa tttgccaaac gaaaattatg gcaggttgtt acgaggcatt ggatgattca 5700 gaggatattc ttcataactc tctagataat gatgaatgta gcacgcattc acataaggtt 5760 tttgctgaca ttcagagtga agaaatttta caacataacc aaaatatgtc tggattggag 5820 aaagtttcta aaatatcacc ttgtgatgtt agtttggaaa cttcagatat atgtaaatgt 5880 agtataggga agcttcataa gtcagtctca tctgcaaata cttgtgggat ttttagcaca 5940 gcaagtggaa aatctgtcca ggtatcagat gcttcattac aaaacgcaag acaagtgttt 6000 tctgaaatag aagatagtac caagcaagtc ttttccaaag tattgtttaa aagtaacgaa 6060 cattcagacc agctcacaag agaagaaaat actgctatac gtactccaga acatttaata 6120 tcccaaaaag gcttttcata taatgtggta aattcatctg ctttctctgg atttagtaca 6180 gcaagtggaa agcaagtttc cattttagaa agttccttac acaaagttaa gggagtgtta 6240 gaggaatttg atttaatcag aactgagcat agtcttcact attcacctac gtctagacaa 6300 aatgtatcaa aaatacttcc tcgtgttgat aagagaaacc cagagcactg tgtaaactca 6360 gaaatggaaa aaacctgcag taaagaattt aaattatcaa ataacttaaa tgttgaaggt 6420 ggttcttcag aaaataatca ctctattaaa gtttctccat atctctctca atttcaacaa 6480 gacaaacaac agttggtatt aggaaccaaa gtgtcacttg ttgagaacat tcatgttttg 6540 ggaaaagaac aggcttcacc taaaaacgta aaaatggaaa ttggtaaaac tgaaactttt 6600 tctgatgttc ctgtgaaaac aaatatagaa gtttgttcta cttactccaa agattcagaa 6660 aactactttg aaacagaagc agtagaaatt gctaaagctt ttatggaaga tgatgaactg 6720 acagattcta aactgccaag tcatgccaca cattctcttt ttacatgtcc cgaaaatgag 6780 gaaatggttt tgtcaaattc aagaattgga aaaagaagag gagagcccct tatcttagtg 6840 ggagaaccct caatcaaaag aaacttatta aatgaatttg acaggataat agaaaatcaa 6900 gaaaaatcct taaaggcttc aaaaagcact ccagatggca caataaaaga tcgaagatg 6960 tttatgcatc atgtttcttt agagccgatt acctgtgtac cctttcgcac aactaaggaa 7020 cgtcaagaga tacagaatcc aaattttacc gcacctggtc aagaatttct gtctaaatct 7080 catttgtatg aacatctgac tttggaaaaa tcttcaagca atttagcagt ttcaggacat 7140 ccattttatc aagtttctgc tacaagaaat gaaaaaatga gacacttgat tactacaggc 7200 agaccaacca aagtctttgt tccacctttt aaaactaaat cacattttca cagagttgaa 7260 cagtgtgtta ggaatattaa cttggaggaa aacagacaaa agcaaaacat tgatggacat 7320 ggctctgatg atagtaaaaa taagattaat gacaatgaga ttcatcagtt taacaaaaac 7380 aactccaatc aagcagtagc tgtaactttc acaaagtgtg aagaagaacc tttagattta 7440 attacaagtc ttcagaatgc cagagatata caggatatgc gaattaagaa gaaacaaagg 7500 caacgcgtct ttccacagcc aggcagtctg tatcttgcaa aaacatccac tctgcctcga 7560 atctctctga aagcagcagt aggaggccaa gttccctctg cgtgttctca taaacagctg 7620 tatacgtatg gcgtttctaa acattgcata aaaattaaca gcaaaaatgc agagtctttt 7680 cagtttcaca ctgaagatta ttttggtaag gaaagtttat ggactggaaa aggaatacag 7740 ttggctgatg gtggatggct cataccctcc aatgatggaa aggctggaaa agaagaattt 7800 tatagggctc tgtgtgacac tccaggtgtg gatccaaagc ttatttctag aatttgggtt 7860 tataatcact atagatggat catatggaaa ctggcagcta tggaatgtgc ctttcctaag 7920 gaatttgcta atagatgcct aagcccagaa agggtgcttc ttcaactaaa atacagatat 7980 gatacggaaa ttgatagaag cagaagatcg gctataaaaa agataatgga aagggatgac 8040 acagctgcaa aaacacttgt tctctgtgtt tctgacataa tttcattgag cgcaaatata 8100 tctgaaactt ctagcaataa aactagtagt gcagataccc aaaaagtggc cattattgaa 8160 cttacagatg ggtggtatgc tgttaaggcc cagttagatc ctcccctctt agctgtctta 8220 aagaatggca gactgacagt tggtcagaag attattcttc atggagcaga actggtgggc 8280 tctcctgatg cctgtacacc tcttgaagcc ccagaatctc ttatgttaaa gatttctgct 8340 aacagtactc ggcctgctcg ctggtatacc aaacttggat tctttcctga ccctagacct 8400 tttcctctgc ccttatcatc gcttttcagt gatggaggaa atgttggttg tgttgatgta 8460 attattcaaa gagcataccc tatacagtgg atggagaaga catcatctgg attatacata 8520 tttcgcaatg aaagagagga agaaaaggaa gcagcaaaat atgtggaggc ccaacaaaag 8580 agactagaag ccttattcac taaaattcag gaggaatttg aagaacatga agaaaacaca 8640 acaaaaccat atttaccatc acgtgcacta acaagacagc aagttcgtgc tttgcaagat 8700 ggtgcagagc tttatgaagc agtgaagaat gcagcagacc cagcttacct tgagggttat 8760 ttcagtgaag agcagttaag agccttgaat aatcacaggc aaatgttgaa tgataagaaa 8820 caagctcaga tccagttgga aattaggaag gccatggaat ctgctgaaca aaaggaacaa 8880 ggtttatcaa gggatgtcac aaccgtgtgg aagttgcgta ttgtaagcta ttcaaaaaaa 8940 gaaaaagatt cagttatact gagtatttgg cgtccatcat cagatttata ttctctgtta 9000 acagaaggaa agagatacag aatttatcat cttgcaactt caaaatctaa aagtaaatct 9060 gaaagagcta acatacagtt agcagcgaca aaaaaaactc agtatcaaca actaccggtt 9120 tcagatgaaa ttttatttca gatttaccag ccacgggagc cccttcactt cagcaaattt 9180 tagatccag actttcagcc atcttgttct gaggtggacc taataggatt tgtcgtttct 9240 gttgtgaaaa aaacaggact tgcccctttc gtctatttgt cagacgaatg ttacaattta 9300 ctggcaataa agttttggat agaccttaat gaggacatta ttaagcctca tatgttaatt 9360 gctgcaagca acctccagtg gcgaccagaa tccaaatcag gccttcttac tttatttgct 9420 ggagattttt ctgtgttttc tgctagtcca aaagagggcc actttcaaga gacattcaac 9480 aaaatgaaaa atactgttga gaatattgac atactttgca atgaagcaga aaacaagctt 9540 atgcatatac tgcatgcaaa tgatcccaag tggtccaccc caactaaaga ctgtacttca 9600 gggccgtaca ctgctcaaat cattcctggt acaggaaaca agcttctgat gtcttctcct 9660 aattgtgaga tatattatca aagtccttta tcactttgta tggccaaaag gaagtctgtt 9720 tccacacctg tctcagccca gatgacttca aagtcttgta aaggggagaa agagattgat 9780 gaccaaaaga actgcaaaaa gagaagagcc ttggatttct tgagtagact gcctttacct 9840 ccacctgtta gtcccatttg tacatttgtt tctccggctg cacagaaggc atttcagcca 9900 ccaaggagtt gtggcaccaa atacgaaaca cccataaaga aaaaagaact gaattctcct 9960 cagatgactc catttaaaaa attcaatgaa atttctcttt tggaaagtaa ttcaatagct 10020 gacgaagaac ttgcattgat aaatacccaa gctcttttgt ctggttcaac aggagaaaaa 10080 caatttatat ctgtcagtga atccactagg actgctccca ccagttcaga agattatctc 10140 agactgaaac gacgttgtac tacatctctg atcaaagaac aggagagttc ccaggccagt 10200 acggaagaat gtgagaaaaa taagcaggac acaattacaa ctaaaaaata tatctaa 10257 <210> 9 <211> 1452 <212> DNA <213> Artificial Sequence <220> <223> BTK <400> 9 atggccgcag tgattctgga gagcatcttt ctgaagcgat cccaacagaa aaagaaaaca 60 tcacctctaa acttcaagaa gcgcctgttt ctcttgaccg tgcacaaact ctcctactat 120 gagtatgact ttgaacgtgg gagaagaggc agtaagaagg gttcaataga tgttgagaag 180 atcacttgtg ttgaaacagt ggttcctgaa aaaaatcctc ctccagaaag acagatccg 240 agaagaggtg aagagtccag tgaaatggag caaatttcaa tcattgaaag gttcccttat 300 cccttccagg ttgtatatga tgaagggcct ctctacgtct tctccccaac tgaagaacta 360 aggaagcggt ggattcacca gctcaaaaac gtaatccggt acaacagtga tctggttcag 420 aaatatcacc cttgcttctg gatcgatggg cagtatctct gctgctctca gacagccaaa 480 aatgctatgg gctgccaaat tttggagaac aggaatggaa gcttaaaacc tgggagttct 540 caccggaaga caaaaaagcc tcttccccca acgcctgagg aggaccagat cttgaaaaag 600 ccactaccgc ctgagccagc agcagcacca gtctccacaa gtgagctgaa aaaggttgtg 660 gccctttatg attacatgcc aatgaatgca aatgatctac agctgcggaa gggtgatgaa 720 tattttatct tggaggaaag caacttacca tggtggagag cacgagataa aaatgggcag 780 gaaggctaca ttcctagtaa ctatgtcact gaagcagaag actccataga aatgtatgag 840 tggtattcca aacacatgac tcggagtcag gctgagcaac tgctaaagca agaggggaaa 900 gaaggaggtt tcattgtcag agactccagc aaagctggca aatatacagt gtctgtgttt 960 gctaaatcca caggggaccc tcaaggggtg atacgtcatt atgttgtgtg ttccacacct 1020 cagagccagt attacctggc agctcgaaac tgtttggtaa acgatcaagg agttgttaaa 1080 gtatctgatt tcggcctgtc caggtatgtc ctggatgatg aatacacaag ctcagtaggc 1140 tccaaatttc cagtccggtg gtccccaccg gaagtcctga tgtatagcaa gttcagcagc 1200 aaatctgaca tttgggcttt tggggttttg atgtgggaaa tttactccct ggggaagatg 1260 ccatatgaga gatttactaa cagtgagact gctgaacaca ttgcccaagg cctacgtctc 1320 tacaggcctc atctggcttc agagaaggta tataccatca tgtacagttg ctggcatgag 1380 aaagcagatg agcgtcccac tttcaaaatt cttctgagca atattctaga tgtcatggat 1440 gaagaatcct ga 1452 <210> 10 <211> 888 <212> DNA <213> Artificial Sequence <220> <223> CCND1 <400> 10 atggaacacc agctcctgtg ctgcgaagtg gaaaccatcc gccgcgcgta ccccgatgcc 60 aacctcctca acgaccgggt gctgcgggcc atgctgaagg cggaggagac ctgcgcgccc 120 tcggtgtcct acttcaaatg tgtgcagaag gaggtcctgc cgtccatgcg gaagatcgtc 180 gccacctgga tgctggaggt ctgcgaggaa cagaagtgcg aggaggaggt cttcccgctg 240 gccatgaact acctggaccg cttcctgtcg ctggagcccg tgaaaaagag ccgcctgcag 300 ctgctggggg ccacttgcat gttcgtggcc tctaagatga aggagaccat ccccctgacg 360 gccgagaagc tgtgcatcta caccgacaac tccatccggc ccgaggagct gctgcaaatg 420 gagctgctcc tggtgaacaa gctcaagtgg aacctggccg caatgacccc gcacgatttc 480 attgaacact tcctctccaa aatgccagag gcggaggaga acaaacagat catccgcaaa 540 cacgcgcaga ccttcgttgc cctctgtgcc acagatgtga agttcatttc caatccgccc 600 tccatggtgg cagcggggag cgtggtggcc gcagtgcaag gcctgaacct gaggagcccc 660 aacaacttcc tgtcctacta ccgcctcaca cgcttcctct ccagagtgat caagtgtgac 720 ccggactgcc tccgggcctg ccaggagcag atcgaagccc tgctggagtc aagcctgcgc 780 caggcccagc agaacatgga ccccaaggcc gccgaggagg aggaagagga ggaggaggag 840 gtggacctgg cttgcacacc caccgacgtg cgggacgtgg acatctga 888 <210> 11 <211> 738 <212> DNA <213> Artificial Sequence <220> <223> CD274 <400> 11 atgaggatat ttgctgtctt tatattcatg acctactggc atttgctgaa cgcatttact 60 gtcacggttc ccaaggacct atatgtggta gagtatggta gcaatatgac aattgaatgc 120 aaattcccag tagaaaaaca attagacctg gctgcactaa ttgtctattg ggaaatggag 180 gataagaaca ttattcaatt tgtgcatgga gaggaagacc tgaaggttca gcatagtagc 240 tacagacaga gggcccggct gttgaaggac cagctctccc tgggaaatgc tgcacttcag 300 atcacagatg tgaaattgca ggatgcaggg gtgtaccgct gcatgatcag ctatggtggt 360 gccgactaca agcgaattac tgtgaaagtc aatgccccat acaacaaaat caaccaaaga 420 attttggttg tggatccagt cacctctgaa catgaactga catgtcaggc tgaggggctac 480 cccaaggccg aagtcatctg gacaagcagt gaccatcaag tcctgagtgg taagaccacc 540 accaccaatt ccaagagaga ggagaagctt ttcaatgtga ccagcacact gagaatcaac 600 acaacaacta atgagatttt ctactgcact tttaggagat tagatcctga ggaaaaccat 660 acagctgaat tggtcatccc aggtaatatt ctgaatgtgt ccattaaaat atgtctaaca 720 ctgtccccta gcacctag 738 <210> 12 <211> 912 <212> DNA <213> Artificial Sequence <220> <223> CDK4 <400> 12 atggctacct ctcgatatga gccagtggct gaaattggtg tcggtgccta tgggacagtg 60 tacaaggccc gtgatcccca cagtggccac tttgtggccc tcaagagtgt gagagtcccc 120 aatggaggag gaggtggagg aggccttccc atcagcacag ttcgtgaggt ggctttactg 180 aggcgactgg aggcttttga gcatcccaat gttgtccggc tgatggacgt ctgtgccaca 240 tcccgaactg accgggagat caaggtaacc ctggtgtttg agcatgtaga ccaggaccta 300 aggacatatc tggacaaggc accccccacca ggcttgccag ccgaaacgat caaggatctg 360 atgcgccagt ttctaagagg cctagatttc cttcatgcca attgcatcgt tcaccgagat 420 ctgaagccag agaacattct ggtgacaagt ggtggaacag tcaagctggc tgactttggc 480 ctggccagaa tctacagcta ccagatggca cttacacccg tggttgttac actctggtac 540 cgagctcccg aagttcttct gcagtccaca tatgcaacac ctgtggacat gtggagtgtt 600 ggctgtatct ttgcagagat gtttcgtcga aagcctctct tctgtggaaa ctctgaagcc 660 gaccagttgg gcaaaatctt tgacctgatt gggctgcctc cagaggatga ctggcctcga 720 gatgtatccc tgccccgtgg agcctttccc cccagagggc cccgcccagt gcagtcggtg 780 gtacctgaga tggaggagtc gggagcacag ctgctgctgg aaatgctgac ttttaaccca 840 cacaagcgaa tctctgcctt tcgagctctg cagcactctt atctacataa ggatgaaggt 900 aatccggagt ga 912 <210> 13 <211> 981 <212> DNA <213> Artificial Sequence <220> <223> CDK6 <400> 13 atggagaagg acggcctgtg ccgcgctgac cagcagtacg aatgcgtggc ggagatcggg 60 gagggcgcct atgggaaggt gttcaaggcc cgcgacttga agaacggagg ccgtttcgtg 120 gcgttgaagc gcgtgcgggt gcagaccggc gaggagggca tgccgctctc caccatccgc 180 gaggtggcgg tgctgaggca cctggagacc ttcgagcacc ccaacgtggt caggttgttt 240 gatgtgtgca cagtgtcacg aacagacaga gaaaccaaac taactttagt gtttgaacat 300 gtcgatcaag acttgaccac ttacttggat aaagttccag agcctggagt gcccactgaa 360 accataaagg atatgatgtt tcagcttctc cgaggtctgg actttcttca ttcacaccga 420 gtagtgcatc gcgatctaaa accacagaac attctggtga ccagcagcgg acaaataaaa 480 ctcgctgact tcggccttgc ccgcatctat agtttccaga tggctctaac ctcagtggtc 540 gtcacgctgt ggtacagagc acccgaagtc ttgctccagt ccagctacgc cacccccgtg 600 gatctctgga gtgttggctg catatttgca gaaatgtttc gtagaaagcc tctttttcgt 660 ggaagttcag atgttgatca actaggaaaa atcttggacg tgattggact cccaggagaa 720 gaagactggc ctagagatgt tgcccttccc aggcaggctt ttcattcaaa atctgcccaa 780 ccaattgaga agtttgtaac agatatcgat gaactaggca aagacctact tctgaagtgt 840 ttgacattta acccagccaa aagaatatct gcctacagtg ccctgtctca cccatacttc 900 caggacctgg aaaggtgcaa agaaaacctg gattcccacc tgccgcccag ccagaacacc 960 tcggagctga atacagcctg a 981 <210> 14 <211> 351 <212> DNA <213> Artificial Sequence <220> <223> CDKN2A <400> 14 atggagccgg cggcggggag cagcatggag ccttcggctg actggctggc cacggccgcg 60 gcccggggtc gggtagagga ggtgcgggcg ctgctggagg cgggggcgct gcccaacgca 120 ccgaatagtt acggtcggag gccgatccag gtgggtagag ggtctgcagc gggagcaggg 180 gatggcgggc gactctggag gacgaagttt gcaggggaat tggaatcagg tagcgcttcg 240 attctccgga aaaaggggag gcttcctggg gagttttcag aaggggtttg taatcacaga 300 cctcctcctg gcgacgccct gggggcttgg gaagccaagg aagaggaatg a 351 <210> 15 <211> 2568 <212> DNA <213> Artificial Sequence <220> <223> DDR2 <400> 15 atgatcctga ttcccagaat gctcttggtg ctgttcctgc tgctgcctat cttgagttct 60 gcaaaagctc aggttaatcc agctatatgc cgctatcctc tgggcatgtc aggaggccag 120 attccagatg aggacatcac agcttccagt cagtggtcag agtccacagc tgccaaatat 180 ggaaggctgg actcagaaga aggggatgga gcctggtgcc ctgagattcc agtggaacct 240 gatgacctga aggagtttct gcagattgac ttgcacaccc tccattttat cactctggtg 300 gggacccagg ggcgccatgc aggaggtcat ggcatcgagt ttgcccccat gtacaagatc 360 aattacagtc gggatggcac tcgctggatc tcttggcgga accgtcatgg gaaacaggtg 420 ctggatggaa atagtaaccc ctatgacatt ttcctaaagg acttggagcc gcccattgta 480 gccagatttg tccggttcat tccagtcacc gaccactcca tgaatgtgtg tatgagagtg 540 gagctttacg gctgtgtctg gctagatggc ttggtgtctt acaatgctcc agctgggcag 600 cagtttgtac tccctggagg ttccatcatt tatctgaatg attctgtcta tgatggagct 660 gttggataca gcatgacaga agggctaggc caattgaccg atggtgtgtc tggcctggac 720 gatttcaccc agacccatga ataccacgtg tggcccggct atgactatgt gggctggcgg 780 aacgagagtg ccaccaatgg ctacattgag atcatgtttg aatttgaccg catcaggaat 840 ttcactacca tgaaggtcca ctgcaacaac atgtttgcta aaggtgtgaa gatctttaag 900 gaggtacagt gctacttccg ctctgaagcc agtgagtggg aacctaatgc catttccttc 960 ccccttgtcc tggatgacgt caaccccagt gctcggtttg tcacggtgcc tctccaccac 1020 cgaatggcca gtgccatcaa gtgtcaatac cattttgcag atacctggat gatgttcagt 1080 gagatcacct tccaatcaga tgctgcaatg tacaacaact ctgaagccct gcccacctct 1140 cctatggcac ccacaaccta tgatccaatg cttaaagttg atgacagcaa cactcggatc 1200 ctgattggct gcttggtggc catcatcttt atcctcctgg ccatcattgt catcatcctc 1260 tggaggcagt tctggcagaa aatgctggag aaggcttctc ggaggatgct ggatgatgaa 1320 atgacagtca gcctttccct gccaagtgat tctagcatgt tcaacaataa ccgctcctca 1380 tcacctagtg aacaagggtc caactcgact tacgatcgca tctttcccct tcgccctgac 1440 taccaggagc catccaggct gatacgaaaa ctcccagaat ttgctccagg ggaggaggag 1500 tcaggctgca gcggtgttgt gaagccagtc cagcccagtg gccctgaggg ggtgccccac 1560 tatgcagagg ctgacatagt gaacctccaa ggagtgacag gaggcaacac atactcagtg 1620 cctgccgtca ccatggacct gctctcagga aaagatgtgg ctgtggagga gttccccagg 1680 aaactcctaa ctttcaaaga gaagctggga gaaggacagt ttggggaggt tcatctctgt 1740 gaagtggagg gaatggaaaa attcaaagac aaagattttg ccctagatgt cagtgccaac 1800 cagcctgtcc tggtggctgt gaaaatgctc cgagcagatg ccaacaagaa tgccaggaat 1860 gattttctta aggagataaa gatcatgtct cggctcaagg acccaaacat catccatcta 1920 ttagctgtgt gtatcactga tgaccctctc tgtatgatca ctgaatacat ggagaatgga 1980 gatctcaatc agtttctttc ccgccacgag ccccctaatt cttcctccag cgatgtacgc 2040 actgtcagtt acaccaatct gaagtttatg gctacccaaa ttgcctctgg catgaagtac 2100 ctttcctctc ttaattttgt tcaccgagat ctggccacac gaaactgttt agtgggtaag 2160 aactacacaa tcaagatagc tgactttgga atgagcagga acctgtacag tggtgactat 2220 taccggatcc agggccgggc agtgctccct atccgctgga tgtcttggga gagtatcttg 2280 ctgggcaagt tcactacagc aagtgatgtg tgggcctttg gggttacttt gtgggagact 2340 ttcacctttt gtcaagaaca gccctattcc cagctgtcag atgaacaggt tattgagaat 2400 actggagagt tcttccgaga ccaagggagg cagacttacc tccctcaacc agccatttgt 2460 cctgactctg tgtataagct gatgctcagc tgctggagaa gagatacgaa gaaccgtccc 2520 tcattccaag aaatccacct tctgctcctt caacaaggcg acgagtga 2568 <210> 16 <211> 3276 <212> DNA <213> Artificial Sequence <220> <223> EGFR <400> 16 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg 60 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag 120 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg 180 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag 240 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct 300 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca 360 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta 420 cagggccaaa agtgtgatcc aagctgtccc aatgggagct gctggggtgc aggagaggag 480 aactgccaga aactgaccaa aatcatctgt gcccagcagt gctccgggcg ctgccgtggc 540 aagtccccca gtgactgctg ccacaaccag tgtgctgcag gctgcacagg cccccgggag 600 agcgactgcc tggtctgccg caaattccga gacgaagcca cgtgcaagga cacctgcccc 660 ccactcatgc tctacaaccc caccacgtac cagatggatg tgaaccccga gggcaaatac 720 agctttggtg ccacctgcgt gaagaagtgt ccccgtaatt atgtggtgac agatcacggc 780 tcgtgcgtcc gagcctgtgg ggccgacagc tatgagatgg aggaagacgg cgtccgcaag 840 tgtaagaagt gcgaagggcc ttgccgcaaa gtgtgtaacg gaataggtat tggtgaattt 900 aaagactcac tctccataaa tgctacgaat attaaacact tcaaaaactg cacctccatc 960 agtggcgatc tccacatcct gccggtggca tttaggggtg actccttcac acatactcct 1020 cctctggatc cacaggaact ggatattctg aaaaccgtaa aggaaatcac agggtttttg 1080 ctgattcagg cttggcctga aaacaggacg gacctccatg cctttgagaa cctagaaatc 1140 atacgcggca ggaccaagca acatggtcag ttttctcttg cagtcgtcag cctgaacata 1200 acatccttgg gattacgctc cctcaaggag ataagtgatg gagatgtgat aatttcagga 1260 aacaaaaatt tgtgctatgc aaatacaata aactggaaaa aactgtttgg gacctccggt 1320 cagaaaacca aaattataag caacagaggt gaaaacagct gcaaggccac aggccaggtc 1380 tgccatgcct tgtgctcccc cgagggctgc tggggcccgg agcccaggga ctgcgtctct 1440 tgccggaatg tcagccgagg cagggaatgc gtggacaagt gcaaccttct ggagggtgag 1500 ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc acccagagtg cctgcctcag 1560 gccatgaaca tcacctgcac aggacgggga ccagacaact gtatccagtg tgcccactac 1620 attgacggcc cccactgcgt caagacctgc ccggcaggag tcatgggaga aaacaacacc 1680 ctggtctgga agtacgcaga cgccggccat gtgtgccacc tgtgccatcc aaactgcacc 1740 tacggatgca ctgggccagg tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc 1800 atcgccactg ggatggtggg ggccctcctc ttgctgctgg tggtggccct ggggatcggc 1860 ctcttcatgc gaaggcgcca catcgttcgg aagcgcacgc tgcggaggct gctgcaggag 1920 agggagcttg tggagcctct tacacccagt ggagaagctc ccaaccaagc tctcttgagg 1980 atcttgaagg aaactgaatt caaaaagatc aaagtgctgg gctccggtgc gttcggcacg 2040 gtgtataagg gactctggat cccagaaggt gagaaagtta aaattcccgt cgctatcaag 2100 gaattaagag aagcaacatc tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg 2160 atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc 2220 gtgcagctca tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac 2280 aaagacaata ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg 2340 aactacttgg aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg 2400 aaaacaccgc agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa 2460 gagaaagaat accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca 2520 attttacaca gaatctatac ccaccagagt gatgtctgga gctacggggt gactgtttgg 2580 gagttgatga cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc 2640 atcctggaga aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg 2700 atcatggtca agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc 2760 atcgaattct ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gggggatgaa 2820 agaatgcatt tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa 2880 gacatggacg acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc 2940 agcccctcca cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc 3000 accgtggctt gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc 3060 ttgcagcgat acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc 3120 ttcctcccag tgcctggtga gtggcttgtc tggaaacagt cctgctcctc aacctcctcg 3180 acccactcag cagcagccag tctccagtgt ccaagccagg tgctccctcc agcatctcca 3240 gagggggaaa cagtggcaga tttgcagaca cagtga 3276 <210> 17 <211> 1812 <212> DNA <213> Artificial Sequence <220> <223> ERBB2 <400> 17 atgaagctgc ggctccctgc cagtcccgag acccacctgg acatgctccg ccacctctac 60 cagggctgcc aggtggtgca gggaaacctg gaactcacct acctgcccac caatgccagc 120 ctgtccttcc tgcaggatat ccaggaggtg cagggctacg tgctcatcgc tcacaaccaa 180 gtgaggcagg tcccactgca gaggctgcgg attgtgcgag gcacccagct ctttgaggac 240 aactatgccc tggccgtgct agacaatgga gacccgctga acaataccac ccctgtcaca 300 ggggcctccc caggaggcct gcgggagctg cagcttcgaa gcctcacaga gatcttgaaa 360 ggaggggtct tgatccagcg gaacccccag ctctgctacc aggacacgat tttgtggaag 420 gacatcttcc acaagaacaa ccagctggct ctcacactga tagacaccaa ccgctctcgg 480 gcctgccacc cctgttctcc gatgtgtaag ggctcccgct gctggggaga gagttctgag 540 gattgtcaga gcctgacgcg cactgtctgt gccggtggct gtgcccgctg caaggggcca 600 ctgcccactg actgctgcca tgagcagtgt gctgccggct gcaggggccc caagcactct 660 gactgcctgg cctgcctcca cttcaaccac agtggcatct gtgagctgca ctgcccagcc 720 ctggtcacct acaacacaga cacgtttgag tccatgccca atcccgaggg ccggtataca 780 ttcggcgcca gctgtgtgac tgcctgtccc tacaactacc tttctacgga cgtgggatcc 840 tgcaccctcg tctgccccct gcacaaccaa gaggtgacag cagaggatgg aacacagcgg 900 tgtgagaagt gcagcaagcc ctgtgcccga gtgtgctatg gtctgggcat ggagcacttg 960 cgagaggtga gggcagttac cagtgccaat atccaggagt ttgctggctg caagaagatc 1020 tttgggagcc tggcatttct gccggagagc tttgatgggg acccagcctc caacactgcc 1080 ccgctccagc cagagcagct ccaagtgttt gagactctgg aagagatcac aggttaccta 1140 tacatctcag catggccgga cagcctgcct gacctcagcg tcttccagaa cctgcaagta 1200 atccggggac gaattctgca caatggcgcc tactcgctga ccctgcaagg gctgggcatc 1260 agctggctgg ggctgcgctc actgagggaa ctgggcagtg gactggccct catccaccat 1320 aacacccacc tctgcttcgt gcacacggtg ccctgggacc agctctttcg gaacccgcac 1380 caagctctgc tccacactgc caaccggcca gaggacgagt gtgtgggcga gggcctggcc 1440 tgccaccagc tgtgcgcccg agggcactgc tggggtccag ggcccaccca gtgtgtcaac 1500 tgcagccagt tccttcgggg ccaggagtgc gtggaggaat gccgagtact gcaggggctc 1560 cccagggagt atgtgaatgc caggcactgt ttgccgtgcc accctgagtg tcagccccag 1620 aatggctcag tgacctgttt tggaccggag gctgaccagt gtgtggcctg tgccccactat 1680 aaggaccctc ccttctgcgt ggcccgctgc cccagcggtg tgaaacctga cctctcctac 1740 atgcccatct ggaagtttcc agatgaggag ggcgcatgcc agccttgccc catcaactgc 1800 acccactcgt ga 1812 <210> 18 <211> 4029 <212> DNA <213> Artificial Sequence <220> <223> ERBB3 <400> 18 atgagggcga acgacgctct gcaggtgctg ggcttgcttt tcagcctggc ccggggctcc 60 gaggtgggca actctcaggc agtgtgtcct gggactctga atggcctgag tgtgaccggc 120 gatgctgaga accaatacca gacactgtac aagctctacg agaggtgtga ggtggtgatg 180 gggaaccttg agattgtgct cacgggacac aatgccgacc tctccttcct gcagtggatt 240 cgagaagtga caggctatgt cctcgtggcc atgaatgaat tctctactct accattgccc 300 aacctccgcg tggtgcgagg gacccaggtc tacgatggga agtttgccat cttcgtcatg 360 ttgaactata acaccaactc cagccacgct ctgcgccagc tccgcttgac tcagctcacc 420 gagattctgt cagggggtgt ttatattgag aagaacgata agctttgtca catggacaca 480 attgactgga gggacatcgt gagggaccga gatgctgaga tagtggtgaa ggacaatggc 540 agaagctgtc ccccctgtca tgaggtttgc aaggggcgat gctggggtcc tggatcagaa 600 gactgccaga cattgaccaa gaccatctgt gctcctcagt gtaatggtca ctgctttggg 660 cccaacccca accagtgctg ccatgatgag tgtgccgggg gctgctcagg ccctcaggac 720 acagactgct ttgcctgccg gcacttcaat gacagtggag cctgtgtacc tcgctgtcca 780 cagcctcttg tctacaacaa gctaactttc cagctggaac ccaatcccca caccaagtat 840 cagtatggag gagtttgtgt agccagctgt ccccataact ttgtggtgga tcaaacatcc 900 tgtgtcaggg cctgtcctcc tgacaagatg gaagtagata aaaatgggct caagatgtgt 960 gagccttgtg ggggactatg tcccaaagcc tgtgagggaa caggctctgg gagccgcttc 1020 cagactgtgg actcgagcaa cattgatgga tttgtgaact gcaccaagat cctgggcaac 1080 ctggactttc tgatcaccgg cctcaatgga gacccctggc acaagatccc tgccctggac 1140 ccagagaagc tcaatgtctt ccggacagta cgggagatca caggttacct gaacatccag 1200 tcctggccgc cccacatgca caacttcagt gttttttcca atttgacaac cattggaggc 1260 agaagcctct acaaccgggg cttctcattg ttgatcatga agaacttgaa tgtcacatct 1320 ctgggcttcc gatccctgaa ggaaattagt gctgggcgta tctatataag tgccaatagg 1380 cagctctgct accaccactc tttgaactgg accaaggtgc ttcgggggcc tacggaagag 1440 cgactagaca tcaagcataa tcggccgcgc agagactgcg tggcagaggg caaagtgtgt 1500 gacccactgt gctcctctgg gggatgctgg ggcccaggcc ctggtcagtg cttgtcctgt 1560 cgaaattata gccgaggagg tgtctgtgtg acccactgca actttctgaa tggggagcct 1620 cgagaatttg cccatgaggc cgaatgcttc tcctgccacc cggaatgcca acccatggag 1680 ggcactgcca catgcaatgg ctcgggctct gatacttgtg ctcaatgtgc ccattttcga 1740 gatgggcccc actgtgtgag cagctgcccc catggagtcc taggtgccaa gggcccaatc 1800 tacaagtacc cagatgttca gaatgaatgt cggccctgcc atgagaactg cacccagggg 1860 tgtaaaggac cagagcttca agactgttta ggacaaacac tggtgctgat cggcaaaacc 1920 catctgacaa tggctttgac agtgatagca ggattggtag tgattttcat gatgctgggc 1980 ggcacttttc tctactggcg tgggcgccgg attcagaata aaagggctat gaggcgatac 2040 ttggaacggg gtgagagcat agagcctctg gaccccagtg agaaggctaa caaagtcttg 2100 gccagaatct tcaaagagac agagctaagg aagcttaaag tgcttggctc gggtgtcttt 2160 ggaactgtgc acaaaggagt gtggatccct gagggtgaat caatcaagat tccagtctgc 2220 attaaagtca ttgaggacaa gagtggacgg cagagttttc aagctgtgac agatcatatg 2280 ctggccattg gcagcctgga ccatgcccac attgtaaggc tgctgggact atgcccaggg 2340 tcatctctgc agcttgtcac tcaatatttg cctctgggtt ctctgctgga tcatgtgaga 2400 caacaccggg gggcactggg gccacagctg ctgctcaact ggggagtaca aattgccaag 2460 ggaatgtact accttgagga acatggtatg gtgcatagaa acctggctgc ccgaaacgtg 2520 ctactcaagt cacccagtca ggttcaggtg gcagattttg gtgtggctga cctgctgcct 2580 cctgatgata agcagctgct atacagtgag gccaagactc caattaagtg gatggccctt 2640 gagagtatcc actttgggaa atacacacac cagagtgatg tctggagcta tggtgtgaca 2700 gtttgggagt tgatgacctt cggggcagag ccctatgcag ggctacgatt ggctgaagta 2760 ccagacctgc tagagaaggg ggagcggttg gcacagcccc agatctgcac aattgatgtc 2820 tacatggtga tggtcaagtg ttggatgatt gatgagaaca ttcgcccaac ctttaaagaa 2880 ctagccaatg agttcaccag gatggcccga gacccaccac ggtatctggt cataaagaga 2940 gagagtgggc ctggaatagc ccctgggcca gagccccatg gtctgacaaa caagaagcta 3000 gaggaagtag agctggagcc agaactagac ctagacctag acttggaagc agaggaggac 3060 aacctggcaa ccaccacact gggctccgcc ctcagcctac cagttggaac acttaatcgg 3120 ccacgtggga gccagagcct tttaagtcca tcatctggat acaggcccat gaaccagggt 3180 aatcttgggg agtcttgcca ggagtctgca gtttctggga gcagtgaacg gtgcccccgt 3240 ccagtctctc tacacccaat gccacgggga tgcctggcat cagagtcatc agaggggcat 3300 gtaacaggct ctgaggctga gctccaggag aaagtgtcaa tgtgtaggag ccggagcagg 3360 agccggagcc cacggccacg cggagatagc gcctaccatt cccagcgcca cagtctgctg 3420 actcctgtta ccccactctc cccacccggg ttagaggaag aggatgtcaa cggttatgtc 3480 atgccagata cacacctcaa aggtactccc tcctccggg aaggcaccct ttcttcagtg 3540 ggtctcagtt ctgtcctggg tactgaagaa gaagatgaag atgaggagta tgaatacatg 3600 aaccggagga gaaggcacag tccacctcat ccccctaggc caagttccct tgaggagctg 3660 ggttatgagt acatggatgt ggggtcagac ctcagtgcct ctctgggcag cacacagagt 3720 tgcccactcc accctgtacc catcatgccc actgcaggca caactccaga tgaagactat 3780 gaatatatga atcggcaacg agatggaggt ggtcctgggg gtgattatgc agccatgggg 3840 gcctgcccag catctgagca agggtatgaa gagatgagag cttttcaggg gcctggacat 3900 caggcccccc atgtccatta tgcccgccta aaaactctac gtagcttaga ggctacagac 3960 tctgcctttg ataaccctga ttactggcat agcaggcttt tccccaaggc taatgcccag 4020 agaacgtaa 4029 <210> 19 <211> 3927 <212> DNA <213> Artificial Sequence <220> <223> ERBB4 <400> 19 atgaagccgg cgacaggact ttgggtctgg gtgagccttc tcgtggcggc ggggaccgtc 60 cagcccagcg attctcagtc agtgtgtgca ggaacggaga ataaactgag ctctctctct 120 gacctggaac agcagtaccg agccttgcgc aagtactatg aaaactgtga ggttgtcatg 180 ggcaacctgg agataaccag cattgagcac aaccgggacc tctccttcct gcggtctgtt 240 cgagaagtca caggctacgt gttagtggct cttaatcagt ttcgttacct gcctctggag 300 aatttacgca ttattcgtgg gacaaaactt tatgaggatc gatatgcctt ggcaatattt 360 ttaaactaca gaaaagatgg aaactttgga cttcaagaac ttggattaaa gaacttgaca 420 gaaatcctaa atggtggagt ctatgtagac cagaacaaat tcctttgtta tgcagacacc 480 attcattggc aagatattgt tcggaaccca tggccttcca acttgactct tgtgtcaaca 540 aatggtagtt caggatgtgg acgttgccat aagtcctgta ctggccgttg ctggggaccc 600 acagaaaatc attgccagac tttgacaagg acggtgtgtg cagaacaatg tgacggcaga 660 tgctacggac cttacgtcag tgactgctgc catcgagaat gtgctggagg ctgctcagga 720 cctaaggaca cagactgctt tgcctgcatg aatttcaatg acagtggagc atgtgttact 780 cagtgtcccc aaacctttgt ctacaatcca accacctttc aactggagca caatttcaat 840 gcaaagtaca catatggagc attctgtgtc aagaaatgtc cacataactt tgtggtagat 900 tccagttctt gtgtgcgtgc ctgccctagt tccaagatgg aagtagaaga aaatgggatt 960 aaaatgtgta aaccttgcac tgacatttgc ccaaaagctt gtgatggcat tggcacagga 1020 tcattgatgt cagctcagac tgtggattcc agtaacattg acaaattcat aaactgtacc 1080 aagatcaatg ggaatttgat ctttctagtc actggtattc atggggaccc ttacaatgca 1140 attgaagcca tagacccaga gaaactgaac gtctttcgga cagtcagaga gataacaggt 1200 ttcctgaaca tacagtcatg gccaccaaac atgactgact tcagtgtttt ttctaacctg 1260 gtgaccattg gtggaagagt actctatagt ggcctgtcct tgcttatcct caagcaacag 1320 ggcatcacct ctctacagtt ccagtccctg aaggaaatca gcgcaggaaa catctatatt 1380 actgacaaca gcaacctgtg ttattatcat accataact ggacaacact cttcagcaca 1440 atcaaccaga gaatagtaat ccgggacaac agaaaagctg aaaattgtac tgctgaagga 1500 atggtgtgca accatctgtg ttccagtgat ggctgttggg gacctgggcc agaccaatgt 1560 ctgtcgtgtc gccgcttcag tagaggaagg atctgcatag agtcttgtaa cctctatgat 1620 ggtgaatttc gggagtttga gaatggctcc atctgtgtgg agtgtgaccc ccagtgtgag 1680 aagatggaag atggcctcct cacatgccat ggaccgggtc ctgacaactg tacaaagtgc 1740 tctcatttta aagatggccc aaactgtgtg gaaaaatgtc cagatggctt acagggggca 1800 aacagtttca ttttcaagta tgctgatcca gatcgggagt gccacccatg ccatccaaac 1860 tgcacccaag ggtgtaacgg tcccactagt catgactgca tttactaccc atggacgggc 1920 cattccactt taccacaaca tgctagaact cccctgattg cagctggagt aattggtggg 1980 ctcttcattc tggtcattgt gggtctgaca tttgctgttt atgttagaag gaagagcatc 2040 aaaaagaaaa gagccttgag aagattcttg gaaacagagt tggtggaacc attaactccc 2100 agtggcacag cacccaatca agctcaactt cgtattttga aagaaactga gctgaagagg 2160 gtaaaagtcc ttggctcagg tgcttttgga acggtttata aaggtatttg ggtacctgaa 2220 ggagaaactg tgaagattcc tgtggctatt aagattctta atgagacaac tggtcccaag 2280 gcaaatgtgg agttcatgga tgaagctctg atcatggcaa gtatggatca tccacaccta 2340 gtccggttgc tgggtgtgtg tctgagccca accatccagc tggttactca acttatgccc 2400 catggctgcc tgttggagta tgtccacgag cacaaggata acatggatc acaactgctg 2460 cttaactggt gtgtccagat agctaaggga atgatgtacc tggaagaaag acgactcgtt 2520 catcgggatt tggcagcccg taatgtctta gtgaaatctc caaaccatgt gaaaatcaca 2580 gattttgggc tagccagact cttggaagga gatgaaaaag agtacaatgc tgatggagga 2640 aagatgccaa ttaaatggat ggctctggag tgtatacatt acaggaaatt cacccatcag 2700 agtgacgttt ggagctatgg agttactata tgggaactga tgacctttgg aggaaaaccc 2760 tatgatggaa ttccaacgcg agaaatccct gattattag agaaaggaga acgtttgcct 2820 cagcctccca tctgcactat tgacgtttac atggtcatgg tcaaatgttg gatgattgat 2880 gctgacagta gacctaaatt taaggaactg gctgctgagt tttcaaggat ggctcgagac 2940 cctcaaagat acctagttat tcagggtgat gatcgtatga agcttcccag tccaaatgac 3000 agcaagttct ttcagaatct cttggatgaa gaggatttgg aagatatgat ggatgctgag 3060 gagtacttgg tccctcaggc tttcaacatc ccacctccca tctatacttc cagagcaaga 3120 attgactcga ataggagtga aattggacac agccctcctc ctgcctacac ccccatgtca 3180 ggaaaccagt ttgtataccg agatggaggt tttgctgctg aacaaggagt gtctgtgccc 3240 tacagagccc caactagcac aattccagaa gctcctgtgg cacagggtgc tactgctgag 3300 atttttgatg actcctgctg taatggcacc ctacgcaagc cagtggcacc ccatgtccaa 3360 gaggacagta gcacccagag gtacagtgct gaccccaccg tgtttgcccc agaacggagc 3420 ccacgaggag agctggatga ggaaggttac atgactccta tgcgagacaa acccaaacaa 3480 gaatacctga atccagtgga ggagaaccct tttgtttctc ggagaaaaaa tggagacctt 3540 caagcattgg ataatcccga atatcacaat gcatccaatg gtccacccaa ggccgaggat 3600 gagtatgtga atgagccact gtacctcaac acctttgcca acaccttggg aaaagctgag 3660 tacctgaaga acaacatact gtcaatgcca gagaaggcca agaaagcgtt tgacaaccct 3720 gactactgga accacagcct gccacctcgg agcacccttc agcacccaga ctacctgcag 3780 gagtacagca caaaatattt ttataaacag aatgggcgga tccggcctat tgtggcagag 3840 aatcctgaat acctctctga gttctccctg aagccaggca ctgtgctgcc gcctccacct 3900 tacagacacc ggaatactgt ggtgtaa 3927 <210> 20 <211> 6357 <212> DNA <213> Artificial Sequence <220> <223> ESR1 <400> 20 gacaaacaga gatatatcgg agtctggcac ggggcacata aggcagcaca ttagagaaag 60 ccggcccctg gatccgtctt tcgcgtttat tttaagccca gtcttccctg ggccaccttt 120 agcagatcct cgtgcgcccc cgccccctgg ccgtgaaact cagcctctat ccagcagcga 180 cgacaagtaa agtggcccgc cggtttctga gccttctgcc ctgcggggac acggtctgca 240 ccctgcccgc ggccacggac catgaccatg accctccaca ccaaagcatc tgggatggcc 300 ctactgcatc agatccaagg gaacgagctg gagcccctga accgtccgca gctcaagatc 360 cccctggagc ggcccctggg cgaggtgtac ctggacagca gcaagcccgc cgtgtacaac 420 taccccgagg gcgccgccta cgagttcaac gccgcggccg ccgccaacgc gcaggtctac 480 ggtcagaccg gcctccccta cggccccggg tctgaggctg cggcgttcgg ctccaacggc 540 ctggggggtt tccccccact caacagcgtg tctccgagcc cgctgatgct actgcacccg 600 ccgccgcagc tgtcgccttt cctgcagccc cacggccagc aggtgcccta ctacctggag 660 aacgagccca gcggctacac ggtgcgcgag gccggcccgc cggcattcta caggccaaat 720 tcagataatc gacgccaggg tggcagagaa agattggcca gtaccaatga caagggaagt 780 atggctatgg aatctgccaa ggagactcgc tactgtgcag tgtgcaatga ctatgcttca 840 ggctaccatt atggagtctg gtcctgtgag ggctgcaagg ccttcttcaa gagaagtatt 900 caaggacata acgactatat gtgtccagcc accaaccagt gcaccattga taaaaacagg 960 aggaagagct gccaggcctg ccggctccgc aaatgctacg aagtgggaat gatgaaaggt 1020 gggatacgaa aagaccgaag aggagggaga atgttgaaac acaagcgcca gagagatgat 1080 ggggagggca ggggtgaagt ggggtctgct ggagacatga gagctgccaa cctttggcca 1140 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac 1200 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct 1260 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag 1320 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat 1380 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg 1440 cgctccatgg agcacccagg gaagctactg tttgctccta acttgctctt ggacaggaac 1500 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct 1560 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg 1620 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac 1680 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca 1740 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac 1800 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg 1860 gtgcccctct atgacctgct gctggagatg ctggacgccc accgcctaca tgcgcccact 1920 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct 1980 acttcatcgc attccttgca aaagtattac atcagggggg aggcagaggg tttccctgcc 2040 acggtctgag agctccctgg ctcccacacg gttcagataa tccctgctgc attttaccct 2100 catcatgcac cactttagcc aaattctgtc tcctgcatac actccggcat gcatccaaca 2160 ccaatggctt tctagatgag tggccattca tttgcttgct cagttcttag tggcacatct 2220 tctgtcttct gttgggaaca gccaaaggga ttccaaggct aaatctttgt aacagctctc 2280 tttccccctt gctatgttac taagcgtgag gattcccgta gctcttcaca gctgaactca 2340 gtctatgggt tggggctcag ataactctgt gcatttaagc tacttgtaga gacccaggcc 2400 tggagagtag acattttgcc tctgataagc actttttaaa tggctctaag aataagccac 2460 agcaaagaat ttaaagtggc tcctttaatt ggtgacttgg agaaagctag gtcaagggtt 2520 tattatagca ccctcttgta ttcctatggc aatgcatcct tttatgaaag tggtacacct 2580 taaagctttt atatgactgt agcagagtat ctggtgattg tcaattcatt ccccctatag 2640 gaatacaagg ggcacacagg gaaggcagat cccctagttg gcaagactat tttaacttga 2700 tacactgcag attcagatgt gctgaaagct ctgcctctgg ctttccggtc atgggttcca 2760 gttaattcat gcctcccatg gacctatgga gagcagcaag ttgatcttag ttaagtctcc 2820 ctatatgagg gataagttcc tgatttttgt ttttattttt gtgttacaaa agaaagccct 2880 ccctccctga acttgcagta aggtcagctt caggacctgt tccagtgggc actgtacttg 2940 gatcttcccg gcgtgtgtgt gccttacaca ggggtgaact gttcactgtg gtgatgcatg 3000 atgagggtaa atggtagttg aaaggagcag gggccctggt gttgcattta gccctggggc 3060 atggagctga acagtacttg tgcaggattg ttgtggctac tagagaacaa gagggaaagt 3120 agggcagaaa ctggatacag ttctgaggca cagccagact tgctcagggt ggccctgcca 3180 caggctgcag ctacctagga acattccttg cagaccccgc attgcccttt gggggtgccc 3240 tgggatccct ggggtagtcc agctcttctt catttcccag cgtggccctg gttggaagaa 3300 gcagctgtca cagctgctgt agacagctgt gttcctacaa ttggcccagc accctggggc 3360 acgggagaag ggtggggacc gttgctgtca ctactcaggc tgactggggc ctggtcagat 3420 tacgtatgcc cttggtggtt tagagataat ccaaaatcag ggtttggttt ggggaagaaa 3480 atcctccccc ttcctccccc gccccgttcc ctaccgcctc cactcctgcc agctcatttc 3540 cttcaatttc ctttgaccta taggctaaaa aagaaaggct cattccagcc acagggcagc 3600 cttccctggg cctttgcttc tctagcacaa ttatgggtta cttccttttt cttaacaaaa 3660 aagaatgttt gatttcctct gggtgacctt attgtctgta attgaaaccc tattgagagg 3720 tgatgtctgt gttagccaat gacccaggtg agctgctcgg gcttctcttg gtatgtcttg 3780 tttggaaaag tggatttcat tcatttctga ttgtccagtt aagtgatcac caaaggactg 3840 agaatctggg agggcaaaaa aaaaaaaaaa gtttttatgt gcacttaaat ttggggacaa 3900 ttttatgtat ctgtgttaag gatatgttta agaacataat tcttttgttg ctgtttgttt 3960 aagaagcacc ttagtttgtt taagaagcac cttatatagt ataatatata tttttttgaa 4020 attacattgc ttgtttatca gacaattgaa tgtagtaatt ctgttctgga tttaatttga 4080 ctgggttaac atgcaaaaac caaggaaaaa tatttagttt tttttttttt ttttgtatac 4140 ttttcaagct accttgtcat gtatacagtc atttatgcct aaagcctggt gattattcat 4200 ttaaatgaag atcacatttc atatcaactt ttgtatccac agtagacaaa atagcactaa 4260 tccagatgcc tattgttgga tactgaatga cagacaatct tatgtagcaa agattatgcc 4320 tgaaaaggaa aattattcag ggcagctaat tttgctttta ccaaaatatc agtagtaata 4380 tttttggaca gtagctaatg ggtcagtggg ttctttttaa tgtttatact tagattttct 4440 tttaaaaaaa ttaaaataaa acaaaaaaaa atttctagga ctagacgatg taataccagc 4500 taaagccaaa caattataca gtggaaggtt ttacattatt catccaatgt gtttctattc 4560 atgttaagat actactacat ttgaagtggg cagagaacat cagatgattg aaatgttcgc 4620 ccaggggtct ccagcaactt tggaaatctc tttgtatttt tacttgaagt gccactaatg 4680 gacagcagat attttctggc tgatgttggt attgggtgta ggaacatgat ttaaaaaaaa 4740 actcttgcct ctgctttccc ccactctgag gcaagttaaa atgtaaaaga tgtgatttat 4800 ctggggggct caggtatggt ggggaagtgg attcaggaat ctggggaatg gcaaatatat 4860 taagaagagt attgaaagta tttggaggaa aatggttaat tctgggtgtg caccagggtt 4920 cagtagagtc cacttctgcc ctggagacca caaatcaact agctccattt acagccattt 4980 ctaaaatggc agcttcagtt ctagagaaga aagaacaaca tcagcagtaa agtccatgga 5040 atagctagtg gtctgtgttt cttttcgcca ttgcctagct tgccgtaatg attctataat 5100 gccatcatgc agcaattatg agaggctagg tcatccaaag agaagaccct atcaatgtag 5160 gttgcaaaat ctaaccccta aggaagtgca gtctttgatt tgatttccct agtaaccttg 5220 cagatatgtt taaccaagcc atagcccatg ccttttgagg gctgaacaaa taagggactt 5280 actgataatt tacttttgat cacattaagg tgttctcacc ttgaaatctt atacactgaa 5340 atggccattg atttaggcca ctggcttaga gtactccttc ccctgcatga cactgattac 5400 aaatactttc ctattcatac tttccaatta tgagatggac tgtgggtact gggagtgatc 5460 actaacacca tagtaatgtc taatattcac aggcagatct gcttggggaa gctagttatg 5520 tgaaaggcaa atagagtcat acagtagctc aaaaggcaac cataattctc tttggtgcag 5580 gtcttgggag cgtgatctag attacactgc accattccca agttaatccc ctgaaaactt 5640 actctcaact ggagcaaatg aactttggtc ccaaatatcc atcttttcag tagcgttaat 5700 tatgctctgt ttccaactgc atttcctttc caattgaatt aaagtgtggc ctcgttttta 5760 gtcatttaaa attgttttct aagtaattgc tgcctctatt atggcacttc aattttgcac 5820 tgtcttttga gattcaagaa aaatttctat tctttttttt gcatccaatt gtgcctgaac 5880 ttttaaaata tgtaaatgct gccatgttcc aaacccatcg tcagtgtgtg tgtttagagc 5940 tgtgcaccct agaaacaaca tattgtccca tgagcaggtg cctgagacac agaccccttt 6000 gcattcacag agaggtcatt ggttatagag acttgaatta ataagtgaca ttatgccagt 6060 ttctgttctc tcacaggtga taaacaatgc tttttgtgca ctacatactc ttcagtgtag 6120 agctcttgtt ttatgggaaa aggctcaaat gccaaattgt gtttgatgga ttaatatgcc 6180 cttttgccga tgcatactat tactgatgtg actcggtttt gtcgcagctt tgctttgttt 6240 aatgaaacac acttgtaaac ctcttttgca ctttgaaaaa gaatccagcg ggatgctcga 6300 gcacctgtaa acaattttct caacctattt gatgttcaaa taaagaatta aactaaa 6357 <210> 21 <211> 2562 <212> DNA <213> Artificial Sequence <220> <223> FGFR1 <400> 21 atggaggcaa gggtcagttt gaaaaggagg atcgagctca ctgtggagta tccatggaga 60 tgtggagcct tgtcaccaac ctctaactgc agaactggga tgtggagctg gaagtgcctc 120 ctcttctggg ctgtgctggt cacagccaca ctctgcaccg ctaggccgtc cccgaccttg 180 cctgaacaag cccagccctg gggagcccct gtggaagtgg agtccttcct ggtccacccc 240 ggtgacctgc tgcagcttcg ctgtcggctg cgggacgatg tgcagagcat caactggctg 300 cgggacgggg tgcagctggc ggaaagcaac cgcacccgca tcacagggga ggaggtggag 360 gtgcaggact ccgtgcccgc agactccggc ctctatgctt gcgtaaccag cagcccctcg 420 ggcagtgaca ccacctactt ctccgtcaat gtttcagatg ctctcccctc ctcggaggat 480 gatgatgatg atgatgactc ctcttcagag gagaaagaaa cagataacac caaaccaaac 540 cccgtagctc catattggac atccccagaa aagatggaaa agaaattgca tgcagtgccg 600 gctgccaaga cagtgaagtt caaatgccct tccagtggga ccccaaaccc cacactgcgc 660 tggttgaaaa atggcaaaga attcaaacct gaccacagaa ttggaggcta caaggtccgt 720 tatgccacct ggagcatcat aatggactct gtggtgccct ctgacaaggg caactacacc 780 tgcattgtgg agaatgagta cggcagcatc aaccacacat accagctgga tgtcgtggag 840 cggtcccctc accggcccat cctgcaagca gggttgcccg ccaacaaaac agtggccctg 900 ggtagcaacg tggagttcat gtgtaaggtg tacagtgacc cgcagccgca catccagtgg 960 ctaaagcaca tcgaggtgaa tgggagcaag attggcccag acaacctgcc ttatgtccag 1020 atcttgaaga ctgctggagt taataccacc gacaaagaga tggaggtgct tcacttaaga 1080 aatgtctcct ttgaggacgc aggggagtat acgtgcttgg cgggtaactc tatcggactc 1140 tcccatcact ctgcatggtt gaccgttctg gaagccctgg aagagaggcc ggcagtgatg 1200 acctcgcccc tgtacctgga gatcatcatc tattgcacag gggccttcct catctcctgc 1260 atggtggggt cggtcatcgt ctacaagatg aagagtggta ccaagaagag tgacttccac 1320 agccagatgg ctgtgcacaa gctggccaag agcatccctc tgcgcagaca ggtaacagtg 1380 tctgctgact ccagtgcatc catgaactct ggggttcttc tggttcggcc atcacggctc 1440 tcctccagtg ggactcccat gctagcaggg gtctctgagt atgagcttcc cgaagaccct 1500 cgctgggagc tgcctcggga cagactggtc ttaggcaaac ccctgggaga gggctgcttt 1560 gggcaggtgg tgttggcaga ggctatcggg ctggacaagg acaaacccaa ccgtgtgacc 1620 aaagtggctg tgaagatgtt gaagtcggac gcaacagaga aagacttgtc agacctgatc 1680 tcagaaatgg agatgatgaa gatgatcggg aagcataaga atatcatcaa cctgctgggg 1740 gcctgcacgc aggatggtcc cttgtatgtc atcgtggagt atgcctccaa gggcaacctg 1800 cgggagtacc tgcaggcccg gaggccccca gggctggaat actgctacaa ccccagccac 1860 aacccagagg agcagctctc ctccaaggac ctggtgtcct gcgcctacca ggtggcccga 1920 ggcatggagt atctggcctc caagaagtgc atacaccgag acctggcagc caggaatgtc 1980 ctggtgacag aggacaatgt gatgaagata gcagactttg gcctcgcacg ggacattcac 2040 cacatcgact actataaaaa gacaaccaac ggccgactgc ctgtgaagtg gatggcaccc 2100 gaggcattat ttgaccggat ctacacccac cagagtgatg tgtggtcttt cggggtgctc 2160 ctgtgggaga tcttcactct gggcggctcc ccataccccg gtgtgcctgt ggaggaactt 2220 ttcaagctgc tgaaggaggg tcaccgcatg gacaagccca gtaactgcac caacgagctg 2280 tacatgatga tgcgggactg ctggcatgca gtgccctcac agagacccac cttcaagcag 2340 ctggtggaag acctggaccg catcgtggcc ttgacctcca accaggagta cctggacctg 2400 tccatgcccc tggaccagta ctcccccagc tttcccgaca cccggagctc tacgtgctcc 2460 tcaggggagg attccgtctt ctctcatgag ccgctgcccg aggagccctg cctgccccga 2520 cacccagccc agcttgccaa tggcggactc aaacgccgct ga 2562 <210> 22 <211> 1782 <212> DNA <213> Artificial Sequence <220> <223> FGFR2 <400> 22 atgtgtttaa acaaaacaaa acagctcttt ggcgttgcta agagactgcc attttggagg 60 aaagagcgat cgcctcaccg gcccatcctc caagccggac tgccggcaaa tgcctccaca 120 gtggtcggag gagacgtaga gtttgtctgc aaggtttaca gtgatgccca gccccacatc 180 cagtggatca agcacgtgga aaagaacggc agtaaatacg ggcccgacgg gctgccctac 240 ctcaaggttc tcaaggccgc cggtgttaac accacggaca aagagattga ggttctctat 300 attcggaatg taacttttga ggacgctggg gaatatacgt gcttggcggg taattctatt 360 gggatatcct ttcactctgc atggttgaca gttctgccag cgcctggaag agaaaaggag 420 attacagctt ccccagacta cctggagata gccatttact gcataggggt cttcttaatc 480 gcctgtatgg tggtaacagt catcctgtgc cgaatgaaga acacgaccaa gaagccagac 540 ttcagcagcc agccggctgt gcacaagctg accaaacgta tccccctgcg gagacaggta 600 acagtttcgg ctgagtccag ctcctccatg aactccaaca ccccgctggt gaggataaca 660 acacgcctct cttcaacggc agacaccccc atgctggcag gggtctccga gtatgaactt 720 ccagaggacc caaaatggga gtttccaaga gataagctga cactgggcaa gcccctggga 780 gaaggttgct ttgggcaagt ggtcatggcg gaagcagtgg gaattgacaa agacaagccc 840 aaggaggcgg tcaccgtggc cgtgaagatg ttgaaagatg atgccacaga gaaagacctt 900 tctgatctgg tgtcagagat ggagatgatg aagatgattg ggaaacacaa gaatatcata 960 aatcttcttg gagcctgcac acaggatggg cctctctatg tcatagttga gtatgcctct 1020 aaaggcaacc tccgagaata cctccgagcc cggaggccac ccgggatgga gtactcctat 1080 gacattaacc gtgttcctga ggagcagatg accttcaagg acttggtgtc atgcacctac 1140 cagctggcca gaggcatgga gtacttggct tcccaaaaat gtattcatcg agatttagca 1200 gccagaaatg ttttggtaac agaaaacaat gtgatgaaaa tagcagactt tggactcgcc 1260 agagatatca acaatataga ctattacaaa aagaccacca atgggcggct tccagtcaag 1320 tggatggctc cagaagccct gtttgataga gtatacactc atcagagtga tgtctggtcc 1380 ttcggggtgt taatgtggga gatcttcact ttagggggct cgccctaccc agggattccc 1440 gtggaggaac tttttaagct gctgaaggaa ggacacagaa tggataagcc agccaactgc 1500 accaacgaac tgtacatgat gatgagggac tgttggcatg cagtgccctc ccagagacca 1560 acgttcaagc agttggtaga agacttggat cgaattctca ctctcacaac caatgaggaa 1620 tacttggacc tcagccaacc tctcgaacag tattcaccta gttaccctga cacaagaagt 1680 tcttgttctt caggagatga ttctgttttt tctccagacc ccatgcctta cgaaccatgc 1740 cttcctcagt atccacacat aaacggcagt gttaaaacat ga 1782 <210> 23 <211> 2085 <212> DNA <213> Artificial Sequence <220> <223> FGFR3 <400> 23 atgggcgccc ctgcctgcgc cctcgcgctc tgcgtggccg tggccatcgt ggccggcgcc 60 tcctcggagt ccttggggac ggagcagcgc gtcgtggggc gagcggcaga agtcccgggc 120 ccagagcccg gccagcagga gcagttggtc ttcggcagcg gggatgctgt ggagctgagc 180 tgtccccccgc ccgggggtgg tcccatgggg cccactgtct gggtcaagga tggcacaggg 240 ctggtgccct cggagcgtgt cctggtgggg ccccagcggc tgcaggtgct gaatgcctcc 300 cacgaggact ccggggccta cagctgccgg cagcggctca cgcagcgcgt actgtgccac 360 ttcagtgtgc gggtgacaga cgctccatcc tcgggagatg acgaagacgg ggaggacgag 420 gctgaggaca caggtgtgga cacaggggcc ccttactgga cacggcccga gcggatggac 480 aagaagctgc tggccgtgcc ggccgccaac accgtccgct tccgctgccc agccgctggc 540 aaccccactc cctccatctc ctggctgaag aacggcaggg agttccgcgg cgagcaccgc 600 attggaggca tcaagctgcg gcatcagcag tggagcctgg tcatggaaag cgtggtgccc 660 tcggaccgcg gcaactacac ctgcgtcgtg gagaacaagt ttggcagcat ccggcagacg 720 tacacgctgg acgtgctgga gcgctccccg caccggccca tcctgcaggc ggggctgccg 780 gccaaccaga cggcggtgct gggcagcgac gtggagttcc actgcaaggt gtacagtgac 840 gcacagcccc acatccagtg gctcaagcac gtggaggtga atggcagcaa ggtgggcccg 900 gacggcacac cctacgttac cgtgctcaag gtgtccctgg agtccaacgc gtccatgagc 960 tccaacacac cactggtgcg catcgcaagg ctgtcctcag gggagggccc cacgctggcc 1020 aatgtctccg agctcgagct gcctgccgac cccaaatggg agctgtctcg ggcccggctg 1080 accctgggca agccccttgg ggagggctgc ttcggccagg tggtcatggc ggaggccatc 1140 ggcattgaca aggaccgggc cgccaagcct gtcaccgtag ccgtgaagat gctgaaagac 1200 gatgccactg acaaggacct gtcggacctg gtgtctgaga tggagatgat gaagatgatc 1260 gggaaacaca aaaacatcat caacctgctg ggcgcctgca cgcagggcgg gcccctgtac 1320 gtgctggtgg agtacgcggc caagggtaac ctgcgggagt ttctgcgggc gcggcggccc 1380 ccgggcctgg actactcctt cgacacctgc aagccgcccg aggagcagct caccttcaag 1440 gacctggtgt cctgtgccta ccaggtggcc cggggcatgg agtacttggc ctcccagaag 1500 tgcatccaca gggacctggc tgcccgcaat gtgctggtga ccgaggacaa cgtgatgaag 1560 atcgcagact tcgggctggc ccgggacgtg cacaacctcg actactacaa gaagacgacc 1620 aacggccggc tgcccgtgaa gtggatggcg cctgaggcct tgtttgaccg agtctacact 1680 caccagagtg acgtctggtc ctttggggtc ctgctctggg agatcttcac gctggggggc 1740 tccccgtacc ccggcatccc tgtggaggag ctcttcaagc tgctgaagga gggccaccgc 1800 atggacaagc ccgccaactg cacacacgac ctgtacatga tcatgcggga gtgctggcat 1860 gccgcgccct cccagaggcc caccttcaag cagctggtgg aggacctgga ccgtgtcctt 1920 accgtgacgt ccaccgacga gtacctggac ctgtcggcgc ctttcgagca gtactccccg 1980 ggtggccagg acacccccag ctccagctcc tcaggggacg actccgtgtt tgccccacgac 2040 ctgctgcccc cggccccacc cagcagtggg ggctcgcgga cgtga 2085 <210> 24 <211> 2409 <212> DNA <213> Artificial Sequence <220> <223> FGFR4 <400> 24 atgcggctgc tgctggccct gttgggggtc ctgctgagtg tgcctgggcc tccagtcttg 60 tccctggagg cctctgagga agtggagctt gagccctgcc tggctcccag cctggagcag 120 caagagcagg agctgacagt agcccttggg cagcctgtgc gtctgtgctg tgggcgggct 180 gagcgtggtg gccactggta caaggagggc agtcgcctgg cacctgctgg ccgtgtacgg 240 ggctggaggg gccgcctaga gattgccagc ttcctacctg aggatgctgg ccgctacctc 300 tgcctggcac gaggctccat gatcgtcctg cagaatctca ccttgattac aggtgactcc 360 ttgacctcca gcaacgatga tgaggacccc aagtcccata gggacccctc gaataggcac 420 agttaccccc agcaagcacc ctactggaca cacccccagc gcatggagaa gaaactgcat 480 gcagtacctg cggggaacac cgtcaagttc cgctgtccag ctgcaggcaa ccccacgccc 540 accatccgct ggcttaagga tggacaggcc tttcatgggg agaaccgcat tggaggcatt 600 cggctgcgcc atcagcactg gagtctcgtg atggagagcg tggtgccctc ggaccgcggc 660 acatacacct gcctggtaga gaacgctgtg ggcagcatcc gctataacta cctgctagat 720 gtgctggagc ggtccccgca ccggcccatc ctgcaggccg ggctcccggc caacaccaca 780 gccgtggtgg gcagcgacgt ggagctgctg tgcaaggtgt acagcgatgc ccagccccac 840 atccagtggc tgaagcacat cgtcatcaac ggcagcagct tcggagccga cggtttcccc 900 tatgtgcaag tcctaaagac tgcagacatc aatagctcag aggtggaggt cctgtacctg 960 cggaacgtgt cagccgagga cgcaggcgag tacacctgcc tcgcaggcaa ttccatcggc 1020 ctctcctacc agtctgcctg gctcacggtg ctgccagagg aggaccccac atggaccgca 1080 gcagcgcccg aggccaggta tacggacatc atcctgtacg cgtcgggctc cctggccttg 1140 gctgtgctcc tgctgctggc cgggctgtat cgagggcagg cgctccacgg ccggcacccc 1200 cgcccgcccg ccactgtgca gaagctctcc cgcttccctc tggcccgaca gttctccctg 1260 gagtcaggct cttccggcaa gtcaagctca tccctggtac gaggcgtgcg tctctcctcc 1320 agcggccccg ccttgctcgc cggcctcgtg agtctagatc tacctctcga cccactatgg 1380 gagttccccc gggacaggct ggtgcttggg aagcccctag gcgagggctg ctttggccag 1440 gtagtacgtg cagaggcctt tggcatggac cctgcccggc ctgaccaagc cagcactgtg 1500 gccgtcaaga tgctcaaaga caacgcctct gacaaggacc tggccgacct ggtctcggag 1560 atggaggtga tgaagctgat cggccgacac aagaacatca tcaacctgct tggtgtctgc 1620 acccaggaag ggcccctgta cgtgatcgtg gagtgcgccg ccaagggaaa cctgcgggag 1680 ttcctgcggg cccggcgccc cccaggcccc gacctcagcc ccgacggtcc tcggagcagt 1740 gaggggccgc tctccttccc agtcctggtc tcctgcgcct accaggtggc ccgaggcatg 1800 cagtatctgg agtcccggaa gtgtatccac cgggacctgg ctgcccgcaa tgtgctggtg 1860 actgaggaca atgtgatgaa gattgctgac tttgggctgg cccgcggcgt ccaccacatt 1920 gactactata agaaaaccag caacggccgc ctgcctgtga agtggatggc gcccgaggcc 1980 ttgtttgacc gggtgtacac acaccagagt gacgtgtggt cttttgggat cctgctatgg 2040 gagatcttca ccctcggggg ctccccgtat cctggcatcc cggtggagga gctgttctcg 2100 ctgctgcggg agggacatcg gatggaccga cccccacact gccccccaga gctgtacggg 2160 ctgatgcgtg agtgctggca cgcagcgccc tcccagaggc ctaccttcaa gcagctggtg 2220 gaggcgctgg acaaggtcct gctggccgtc tctgaggagt acctcgacct ccgcctgacc 2280 ttcggaccct attccccctc tggtggggac gccagcagca cctgctcctc cagcgattct 2340 gtcttcagcc acgaccccct gccattggga tccagctcct tccccttcgg gtctggggtg 2400 cagacatga 2409 <210> 25 <211> 2982 <212> DNA <213> Artificial Sequence <220> <223> FLT3 <400> 25 atgccggcgt tggcgcgcga cggcggccag ctgccgctgc tcgttgtttt ttctgcaatg 60 atatttggga ctattacaaa tcaagatctg cctgtgatca agtgtgtttt aatcaatcat 120 aagaacaatg attcatcagt ggggaagtca tcatcatatc ccatggtatc agaatccccg 180 gaagacctcg ggtgtgcgtt gagaccccag agctcaggga cagtgtacga agctgccgct 240 gtggaagtgg atgtatctgc ttccatcaca ctgcaagtgc tggtcgacgc cccagggaac 300 atttcctgtc tctgggtctt taagcacagc tccctgaatt gccagccaca ttttgattta 360 caaaacagag gagttgtttc catggtcatt ttgaaaatga cagaaaccca agctggagaa 420 tacctacttt ttattcagag tgaagctacc aattacacaa tattgtttac agtgagtata 480 agaaataccc tgctttacac attaagaaga ccttacttta gaaaaatgga aaaccaggac 540 gccctggtct gcatatctga gagcgttcca gagccgatcg tggaatgggt gctttgcgat 600 tcacaggggg aaagctgtaa agaagaaagt ccagctgttg ttaaaaagga ggaaaaagtg 660 cttcatgaat tatttgggac ggacataagg tgctgtgcca gaaatgaact gggcagggaa 720 tgcaccaggc tgttcacaat agatctaaat caaactcctc agaccacatt gccacaatta 780 tttcttaaag taggggaacc cttatggata aggtgcaaag ctgttcatgt gaaccatgga 840 ttcgggctca cctgggaatt agaaaacaaa gcactcgagg agggcaacta ctttgagatg 900 agtacctatt caacaaacag aactatgata cggattctgt ttgcttttgt atcatcagtg 960 gcaagaaacg acaccggata ctacacttgt tcctcttcaa agcatcccag tcaatcagct 1020 ttggttacca tcgtagaaaa gggatttata aatgctacca attcaagtga agattatgaa 1080 attgaccaat atgaagagtt ttgtttttct gtcaggttta aagcctaccc acaaatcaga 1140 tgtacgtgga ccttctctcg aaaatcattt ccttgtgagc aaaagggtct tgataacgga 1200 tacagcatat ccaagttttg caatcataag caccagccag gagaatatat attccatgca 1260 gaaaatgatg atgcccaatt taccaaaatg ttcacgctga atataagaag gaaacctcaa 1320 gtgctcgcag aagcatcggc aagtcaggcg tcctgtttct cggatggata cccattacca 1380 tcttggacct ggaagaagtg ttcagacaag tctcccaact gcacagaaga gatcacagaa 1440 ggagtctgga atagaaaggc taacagaaaa gtgtttggac agtgggtgtc gagcagtact 1500 ctaaacatga gtgaagccat aaaagggttc ctggtcaagt gctgtgcata caattccctt 1560 ggcacatctt gtgagacgat ccttttaaac tctccaggcc ccttcccttt catccaagac 1620 aacatctcat tctatgcaac aattggtgtt tgtctcctct tcattgtcgt tttaaccctg 1680 ctaatttgtc acaagtacaa aaagcaattt aggtatgaaa gccagctaca gatggtacag 1740 gtgaccggct cctcagataa tgagtacttc tacgttgatt tcagagaata tgaatatgat 1800 ctcaaatggg agtttccaag agaaaattta gagtttggga aggtactagg atcaggtgct 1860 tttggaaaag tgatgaacgc aacagcttat ggaattagca aaacaggagt ctcaatccag 1920 gttgccgtca aaatgctgaa agaaaaagca gacagctctg aaagagaggc actcatgtca 1980 gaactcaaga tgatgaccca gctgggaagc cacgagaata ttgtgaacct gctgggggcg 2040 tgcacactgt caggaccaat ttacttgatt tttgaatact gttgctatgg tgatcttctc 2100 aactatctaa gaagtaaaag agaaaaattt cacaggactt ggacagagat tttcaaggaa 2160 cacaatttca gtttttaccc cactttccaa tcacatccaa attccagcat gcctggttca 2220 agagaagttc agatacaccc ggactcggat caaatctcag ggcttcatgg gaattcattt 2280 cactctgaag atgaaattga atatgaaaac caaaaaaggc tggaagaaga ggaggacttg 2340 aatgtgctta catttgaaga tcttctttgc tttgcatatc aagttgccaa aggaatggaa 2400 tttctggaat ttaagtcgtg tgttcacaga gacctggccg ccaggaacgt gcttgtcacc 2460 cacgggaaag tggtgaagat atgtgacttt ggattggctc gagatatcat gagtgattcc 2520 aactatgttg tcaggggcaa tgcccgtctg cctgtaaaat ggatggcccc cgaaagcctg 2580 tttgaaggca tctacaccat taagagtgat gtctggtcat atggaatatt actgtgggaa 2640 atcttctcac ttggtgtgaa tccttaccct ggcattccgg ttgatgctaa cttctacaaa 2700 ctgattcaaa atggatttaa aatggatcag ccattttatg ctacagaaga aatatacatt 2760 ataatgcaat cctgctgggc ttttgactca aggaaacggc catccttccc taatttgact 2820 tcgtttttag gatgtcagct ggcagatgca gaagaagcga tgtatcagaa tgtggatggc 2880 cgtgtttcgg aatgtcctca cacctaccaa aacaggcgac ctttcagcag agagatggat 2940 ttggggctac tctctccgca ggctcaggtc gaagattcgt ag 2982 <210> 26 <211> 1080 <212> DNA <213> Artificial Sequence <220> <223> GNA11 <400> 26 atgactctgg agtccatgat ggcgtgttgc ctgagcgatg aggtgaagga gtccaagcgg 60 atcaacgccg agatcgagaa gcagctgcgg cgggacaagc gcgacgcccg gcgcgagctc 120 aagctgctgc tgctcggcac gggcgagagc gggaagagca cgttcatcaa gcagatgcgc 180 atcatccacg gcgccggcta ctcggaggag gacaagcgcg gcttcaccaa gctcgtctac 240 cagaacatct tcaccgccat gcaggccatg atccgggcca tggagacgct caagatcctc 300 tacaagtacg agcagaacaa ggccaatgcg ctcctgatcc gggaggtgga cgtggagaag 360 gtgaccacct tcgagcatca gtacgtcagt gccatcaaga ccctgtggga ggacccgggc 420 atccaggaat gctacgaccg caggcgcgag taccagctct ccgactctgc caagtactac 480 ctgaccgacg ttgaccgcat cgccaccttg ggctacctgc ccacccagca ggacgtgctg 540 cgggtccgcg tgcccaccac cggcatcatc gagtaccctt tcgacctgga gaacatcatc 600 ttccggatgg tggatgtggg gggccagcgg tcggagcgga ggaagtggat ccactgcttt 660 gagaacgtga catccatcat gtttctcgtc gccctcagcg aatacgacca agtcctggtg 720 gagtcggaca acgagaaccg gatggaggag agcaaagccc tgttccggac catcatcacc 780 tacccctggt tccagaactc ctccgtcatc ctcttcctca acaagaagga cctgctggag 840 gacaagatcc tgtactcgca cctggtggac tacttccccg agttcgatgg tccccagcgg 900 gacgcccagg cggcgcggga gttcatcctg aagatgttcg tggacctgaa ccccgacagc 960 gacaagatca tctactcaca cttcacgtgt gccaccgaca cggagaacat ccgcttcgtg 1020 ttcgcggccg tgaaggacac catcctgcag ctcaacctca aggagtacaa cctggtctga 1080 1080 <210> 27 <211> 1080 <212> DNA <213> Artificial Sequence <220> <223> GNAQ <400> 27 atgactctgg agtccatcat ggcgtgctgc ctgagcgagg aggccaagga agcccggcgg 60 atcaacgacg agatcgagcg gcagctccgc agggacaagc gggacgcccg ccgggagctc 120 aagctgctgc tgctcgggac aggagagagt ggcaagagta cgtttatcaa gcagatgaga 180 atcatccatg ggtcaggata ctctgatgaa gataaaaggg gcttcaccaa gctggtgtat 240 cagaacatct tcacggccat gcaggccatg atcagagcca tggacacact caagatccca 300 tacaagtatg agcacaataa ggctcatgca caattagttc gagaagttga tgtggagaag 360 gtgtctgctt ttgagaatcc atatgtagat gcaataaaga gtttatggaa tgatcctgga 420 atccaggaat gctatgatag acgacgagaa tatcaattat ctgactctac caaatactat 480 cttaatgact tggaccgcgt agctgaccct gcctacctgc ctacgcaaca agatgtgctt 540 agagttcgag tccccaccac agggatcatc gaatacccct ttgacttaca aagtgtcatt 600 ttcagaatgg tcgatgtagg gggccaaagg tcagagagaa gaaaatggat acactgcttt 660 gaaaatgtca cctctatcat gtttctagta gcgcttagtg aatatgatca agttctcgtg 720 gagtcagaca atgagaaccg aatggaggaa agcaaggctc tctttagaac aattatcaca 780 tacccctggt tccagaactc ctcggttatt ctgttcttaa acaagaaaga tcttctagag 840 gagaaaatca tgtattccca tctagtcgac tacttcccag aatatgatgg accccagaga 900 gatgcccagg cagcccgaga attcattctg aagatgttcg tggacctgaa cccagacagt 960 gacaaaatta tctactccca cttcacgtgc gccacagaca ccgagaatat ccgctttgtc 1020 tttgctgccg tcaaggacac catcctccag ttgaacctga aggagtacaa tctggtctaa 1080 1080 <210> 28 <211> 513 <212> DNA <213> Artificial Sequence <220> <223> HRAS <400> 28 atgacggaat ataagctggt ggtggtgggc gccggcggtg tgggcaagag tgcgctgacc 60 atccagctga tccagaacca ttttgtggac gaatacgacc ccactataga ggattcctac 120 cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgccggc 180 caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt 240 gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc 300 aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg 360 gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc 420 tacatcgaga cctcggccaa gacccggcag ggcagccgct ctggctctag ctccagctcc 480 gggaccctct gggacccccc gggacccatg tga 513 <210> 29 <211> 1245 <212> DNA <213> Artificial Sequence <220> <223> IDH1 <400> 29 atgtccaaaa aaatcagtgg cggttctgtg gtagagatgc aaggagatga aatgacacga 60 atcatttggg aattgattaa agagaaactc atttttccct acgtggaatt ggatctacat 120 agctatgatt taggcataga gaatcgtgat gccaccaacg accaagtcac caaggatgct 180 gcagaagcta taaagaagca taatgttggc gtcaaatgtg ccactatcac tcctgatgag 240 aagagggttg aggagttcaa gttgaaacaa atgtggaaat caccaaatgg caccatacga 300 aatattctgg gtggcacggt cttcagagaa gccattatct gcaaaaatat cccccggctt 360 gtgagtggat gggtaaaacc tatcatcata ggtcgtcatg cttatgggga tcaatacaga 420 gcaactgatt ttgttgttcc tgggcctgga aaagtagaga taacctacac accaagtgac 480 ggaacccaaa aggtgacata cctggtacat aactttgaag aaggtggtgg tgttgccatg 540 gggatgtata atcaagataa gtcaattgaa gattttgcac acagttcctt ccaaatggct 600 ctgtctaagg gttggccttt gtatctgagc accaaaaaca ctattctgaa gaaatatgat 660 gggcgtttta aagacatctt tcaggagata tatgacaagc agtacaagtc ccagtttgaa 720 gctcaaaaga tctggtatga gcataggctc atcgacgaca tggtggccca agctatgaaa 780 tcagagggag gcttcatctg ggcctgtaaa aactatgatg gtgacgtgca gtcggactct 840 gtggcccaag ggtatggctc tctcggcatg atgaccagcg tgctggtttg tccagatggc 900 aagacagtag aagcagaggc tgcccacggg actgtaaccc gtcactaccg catgtaccag 960 aaaggacagg agacgtccac caatcccatt gcttccattt ttgcctggac cagagggtta 1020 gcccacagag caaagcttga taacaataaa gagcttgcct tctttgcaaa tgctttggaa 1080 gaagtctcta ttgagacaat tgaggctggc ttcatgacca aggacttggc tgcttgcatt 1140 aaaggtttac ccaatgtgca acgttctgac tacttgaata catttgagtt catggataaa 1200 cttggagaaa acttgaagat caaactagct caggccaaac tttaa 1245 <210> 30 <211> 1203 <212> DNA <213> Artificial Sequence <220> <223> IDH2 <400> 30 atggatggtg atgagatgac ccgtattatc tggcagttca tcaaggagaa gctcatcctg 60 ccccacgtgg acatccagct aaagtatttt gacctcgggc tcccaaaccg tgaccagact 120 gatgaccagg tcaccattga ctctgcactg gccacccaga agtacagtgt ggctgtcaag 180 tgtgccacca tcacccctga tgaggcccgt gtggaagagt tcaagctgaa gaagatgtgg 240 aaaagtccca atggaactat ccggaacatc ctggggggga ctgtcttccg ggagcccatc 300 atctgcaaaa acatcccacg cctagtccct ggctggacca agcccatcac cattggcagg 360 cacgcccatg gcgaccagta caaggccaca gactttgtgg cagaccgggc cggcactttc 420 aaaatggtct tcaccccaaa agatggcagt ggtgtcaagg agtgggaagt gtacaacttc 480 cccgcaggcg gcgtgggcat gggcatgtac aacaccgacg agtccatctc aggttttgcg 540 cacagctgct tccagtatgc catccagaag aaatggccgc tgtacatgag caccaagaac 600 accatactga aagcctacga tgggcgtttc aaggacatct tccaggagat ctttgacaag 660 cactataaga ccgacttcga caagaataag atctggtatg agcaccggct cattgatgac 720 atggtggctc aggtcctcaa gtcttcgggt ggctttgtgt gggcctgcaa gaactatgac 780 ggagatgtgc agtcagacat cctggcccag ggctttggct cccttggcct gatgacgtcc 840 gtcctggtct gccctgatgg gaagacgatt gaggctgagg ccgctcatgg gaccgtcacc 900 cgccactatc gggagcacca gaagggccgg cccaccagca ccaaccccat cgccagcatc 960 tttgcctgga cacgtggcct ggagcaccgg gggaagctgg atgggaacca agacctcatc 1020 aggtttgccc agatgctgga gaaggtgtgc gtggagacgg tggagagtgg agccatgacc 1080 aaggacctgg cgggctgcat tcacggcctc agcaatgtga agctgaacga gcacttcctg 1140 aacaccacgg acttcctcga caccatcaag agcaacctgg acagagccct gggcaggcag 1200 tag 1203 <210> 31 <211> 2919 <212> DNA <213> Artificial Sequence <220> <223> KIT <400> 31 atgagaggcg ctcgcggcgc ctgggatttt ctctgcgttc tgctcctact gcttcgcgtc 60 cagacaggct cttctcaacc atctgtgagt ccaggggaac cgtctccacc atccatccat 120 ccaggaaaat cagacttaat agtccgcgtg ggcgacgaga ttaggctgtt atgcactgat 180 ccgggctttg tcaaatggac ttttgagatc ctggatgaaa cgaatgagaa taagcagaat 240 gaatggatca cggaaaaggc agaagccacc aacaccggca aatacacgtg caccaacaaa 300 cacggcttaa gcaattccat ttatgtgttt gttagagatc ctgccaagct tttccttgtt 360 gaccgctcct tgtatgggaa agaagacaac gacacgctgg tccgctgtcc tctcacagac 420 ccagaagtga ccaattattc cctcaagggg tgccagggga agcctcttcc caaggacttg 480 aggtttattc ctgaccccaa ggcgggcatc atgatcaaaa gtgtgaaacg cgcctaccat 540 cggctctgtc tgcattgttc tgtggaccag gagggcaagt cagtgctgtc ggaaaaattc 600 atcctgaaag tgaggccagc cttcaaagct gtgcctgttg tgtctgtgtc caaagcaagc 660 tatcttctta gggaagggga agaattcaca gtgacgtgca caataaaaga tgtgtctagt 720 tctgtgtact caacgtggaa aagagaaaac agtcagacta aactacagga gaaatataat 780 agctggcatc acggtgactt caattatgaa cgtcaggcaa cgttgactat cagttcagcg 840 agagttaatg attctggagt gttcatgtgt tatgccaata atacttttgg atcagcaaat 900 gtcacaacaa ccttggaagt agtagataaa ggattcatta atatcttccc catgataaac 960 actacagtat ttgtaaacga tggagaaaat gtagatttga ttgttgaata tgaagcattc 1020 cccaaacctg aacaccagca gtggatctat atgaacagaa ccttcactga taaatgggaa 1080 gattatccca agtctgagaa tgaaagtaat atcagatacg taagtgaact tcatctaacg 1140 agattaaaag gcaccgaagg aggcacttac acatcctag tgtccaattc tgacgtcaat 1200 gctgccatag catttaatgt ttatgtgaat acaaaaccag aaatcctgac ttacgacagg 1260 ctcgtgaatg gcatgctcca atgtgtggca gcaggattcc cagagcccac aatagattgg 1320 tatttttgtc caggaactga gcagagatgc tctgcttctg tactgccagt ggatgtgcag 1380 acactaaact catctgggcc accgtttgga aagctagtgg ttcagagttc tatagattct 1440 agtgcattca agcacaatgg cacggttgaa tgtaaggctt acaacgatgt gggcaagact 1500 tctgcctatt ttaactttgc atttaaagag caaatccatc cccacaccct gttcactcct 1560 ttgctgattg gtttcgtaat cgtagctggc atgatgtgca ttattgtgat gattctgacc 1620 tacaaatatt tacagaaacc catgtatgaa gtacagtgga aggttgttga ggagataaat 1680 ggaaacaatt atgtttacat agacccaaca caacttcctt atgatcacaa atgggagttt 1740 cccagaaaca ggctgagttt tgggaaaacc ctgggtgctg gagctttcgg gaaggttgtt 1800 gaggcaactg cttatggctt aattaagtca gatgcggcca tgactgtcgc tgtaaagatg 1860 ctcaagccga gtgcccattt gacagaacgg gaagccctca tgtctgaact caaagtcctg 1920 agttaccttg gtaatcacat gaatattgtg aatctacttg gagcctgcac cattggaggg 1980 cccaccctgg tcattacaga atattgttgc tatggtgatc ttttgaattt tttgagaaga 2040 aaacgtgatt catttatttg ttcaaagcag gaagatcatg cagaagctgc actttataag 2100 aatcttctgc attcaaagga gtcttcctgc agcgatagta ctaatgagta catggacatg 2160 aaacctggag tttcttatgt tgtcccaacc aaggccgaca aaaggagatc tgtgagaata 2220 ggctcataca tagaaagaga tgtgactccc gccatcatgg aggatgacga gttggcccta 2280 gacttagaag acttgctgag cttttcttac caggtggcaa agggcatggc tttcctcgcc 2340 tccaagaatt gtattcacag agacttggca gccagaaata tcctccttac tcatggtcgg 2400 atcacaaaga tttgtgattt tggtctagcc agagacatca agaatgattc taattatgtg 2460 gttaaaggaa acgctcgact acctgtgaag tggatggcac ctgaaagcat tttcaactgt 2520 gtatacacgt ttgaaagtga cgtctggtcc tatgggattt ttctttggga gctgttctct 2580 ttaggaagca gcccctatcc tggaatgccg gtcgattcta agttctacaa gatgatcaag 2640 gaaggcttcc ggatgctcag ccctgaacac gcacctgctg aaatgtatga cataatgaag 2700 acttgctggg atgcagatcc cctaaaaaga ccaacattca agcaaattgt tcagctaatt 2760 gagaagcaga tttcagagag caccaatcat atttactcca acttagcaaa ctgcagcccc 2820 aaccgacaga agcccgtggt agaccattct gtgcggatca attctgtcgg cagcaccgct 2880 tcctcctccc agcctctgct tgtgcacgac gatgtctga 2919 <210> 32 <211> 570 <212> DNA <213> Artificial Sequence <220> <223> KRAS <400> 32 atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag agagtggagg atgcttttta tacattggtg 480 agagagatcc gacaatacag attgaaaaaa atcagcaaag aagaaaagac tcctggctgt 540 gtgaaaatta aaaaatgcat tataatgtaa 570 <210> 33 <211> 1182 <212> DNA <213> Artificial Sequence <220> <223> MAP2K1 <400> 33 atgcccaaga agaagccgac gcccatccag ctgaacccgg cccccgacgg ctctgcagtt 60 aacgggacca gctctgcgga gaccaacttg gaggccttgc agaagaagct ggaggagcta 120 gagcttgatg agcagcagcg aaagcgcctt gaggcctttc ttacccagaa gcagaaggtg 180 ggagaactga aggatgacga ctttgagaag atcagtgagc tgggggctgg caatggcggt 240 gtggtgttca aggtctccca caagccttct ggcctggtca tggccagaaa gctaattcat 300 ctggagatca aacccgcaat ccggaaccag atcataaggg agctgcaggt tctgcatgag 360 tgcaactctc cgtacatcgt gggcttctat ggtgcgttct acagcgatgg cgagatcagt 420 atctgcatgg agcacatgga tggaggttct ctggatcaag tcctgaagaa agctggaaga 480 attcctgaac aaattttagg aaaagttagc attgctgtaa taaaaggcct gacatatctg 540 agggagaagc acaagatcat gcacagagat gtcaagccct ccaacatcct agtcaactcc 600 cgtggggaga tcaagctctg tgactttggg gtcagcgggc agctcatcga ctccatggcc 660 aactccttcg tgggcacaag gtcctacat tcgccagaaa gactccaggg gactcattac 720 tctgtgcagt cagacatctg gagcatggga ctgtctctgg tagagatggc ggttgggagg 780 tatcccatcc ctcctccaga tgccaaggag ctggagctga tgtttgggtg ccaggtggaa 840 ggagatgcgg ctgagacccc acccaggcca aggacccccg ggaggcccct tagctcatac 900 ggaatggaca gccgacctcc catggcaatt tttgagttgt tggattacat agtcaacgag 960 cctcctccaa aactgcccag tggagtgttc agtctggaat ttcaagattt tgtgaataaa 1020 tgcttaataa aaaaccccgc agagagagca gatttgaagc aactcatggt tcatgctttt 1080 atcaagagat ctgatgctga ggaagtggat tttgcaggtt ggctctgctc caccatcggc 1140 cttaaccagc ccagcacacc aacccatgct gctggcgtct aa 1182 <210> 34 <211> 2883 <212> DNA <213> Artificial Sequence <220> <223> MET <400> 34 atgggtcaat tcagcgaagt cctcttaaca tctatatcca ccttcattaa aggagacctc 60 accatagcta atcttgggac atcagagggt cgcttcatgc aggttgtggt ttctcgatca 120 ggaccatcaa cccctcatgt gaattttctc ctggactccc atccagtgtc tccagaagtg 180 attgtggagc atacattaaa ccaaaatggc tacacactgg ttatcactgg gaagaagatc 240 acgaagatcc cattgaatgg cttgggctgc agacatttcc agtcctgcag tcaatgcctc 300 tctgccccac cctttgttca gtgtggctgg tgccacgaca aatgtgtgcg atcggaggaa 360 tgcctgagcg ggacatggac tcaacagatc tgtctgcctg caatctacaa ggttttccca 420 aatagtgcac cccttgaagg agggacaagg ctgaccatat gtggctggga ctttggattt 480 cggaggaata ataaatttga tttaaagaaa actagagttc tccttggaaa tgagagctgc 540 accttgactt taagtgagag cacgatgaat acatgaaat gcacagttgg tcctgccatg 600 aataagcatt tcaatatgtc cataattatt tcaaatggcc acgggacaac acaatacagt 660 acattctcct atgtggatcc tgtaataaca agtatttcgc cgaaatacgg tcctatggct 720 ggtggcactt tacttacttt aactggaaat tacctaaaca gtgggaattc tagacacatt 780 tcaattggtg gaaaaacatg tactttaaaa agtgtgtgtcaa acagtattct tgaatgttat 840 accccagccc aaaccatttc aactgagttt gctgttaaat tgaaaattga cttagccaac 900 cgagagacaa gcatcttcag ttaccgtgaa gatcccattg tctatgaaat tcatccaacc 960 aaatctttta ttagtggtgg gagcacaata acaggtgttg ggaaaaacct gaattcagtt 1020 agtgtcccga gaatggtcat aaatgtgcat gaagcaggaa ggaactttac agtggcatgt 1080 caacatcgct ctaattcaga gataatctgt tgtaccactc cttccctgca acagctgaat 1140 ctgcaactcc ccctgaaaac caaagccttt ttcatgttag atgggatcct ttccaaatac 1200 tttgatctca tttatgtaca taatcctgtg tttaagcctt ttgaaaagcc agtgatgatc 1260 tcaatgggca atgaaaatgt actggaaatt aagggaaatg atattgaccc tgaagcagtt 1320 aaaggtgaag tgttaaaagt tggaaataag agctgtgaga atatacactt acattctgaa 1380 gccgttttat gcacggtccc caatgacctg ctgaaattga acagcgagct aaatatagag 1440 tggaagcaag caatttcttc aaccgtcctt ggaaaagtaa tagttcaacc agatcagaat 1500 ttcacaggat tgattgctgg tgttgtctca atatcaacag cactgttatt actacttggg 1560 tttttcctgt ggctgaaaaa gagaaagcaa attaaagatc tgggcagtga attagttcgc 1620 tacgatgcaa gagtacacac tcctcatttg gataggcttg taagtgcccg aagtgtaagc 1680 ccaactacag aaatggtttc aaatgaatct gtagactacc gagctacttt tccagaagat 1740 cagtttccta attcatctca gaacggttca tgccgacaag tgcagtatcc tctgacagac 1800 atgtccccca tcctaactag tggggactct gatatatcca gtccattact gcaaaatact 1860 gtccacattg acctcagtgc tctaaatcca gagctggtcc aggcagtgca gcatgtagtg 1920 attgggccca gtagcctgat tgtgcatttc aatgaagtca taggaagagg gcattttggt 1980 tgtgtatatc atgggacttt gttggacaat gatggcaaga aaattcactg tgctgtgaaa 2040 tccttgaaca gaatcactga cataggagaa gtttcccaat ttctgaccga gggaatcatc 2100 atgaaagatt ttagtcatcc caatgtcctc tcgctcctgg gaatctgcct gcgaagtgaa 2160 gggtctccgc tggtggtcct accatacatg aaacatggag atcttcgaaa tttcattcga 2220 aatgagactc ataatccaac tgtaaaagat cttattggct ttggtcttca agtagccaaa 2280 ggcatgaaat atcttgcaag caaaaagttt gtccacagag acttggctgc aagaaactgt 2340 atgctggatg aaaaattcac agtcaaggtt gctgattttg gtcttgccag agacatgtat 2400 gataaagaat actatagtgt acacaacaaa acaggtgcaa agctgccagt gaagtggatg 2460 gctttggaaa gtctgcaaac tcaaaagttt accaccaagt cagatgtgtg gtcctttggc 2520 gtgctcctct gggagctgat gacaagagga gccccacctt atcctgacgt aaacaccttt 2580 gatataactg tttacttgtt gcaagggaga agactcctac aacccgaata ctgcccagac 2640 cccttatatg aagtaatgct aaaatgctgg caccctaaag ccgaaatgcg cccatccttt 2700 tctgaactgg tgtcccggat atcagcgatc ttctctactt tcattgggga gcactatgtc 2760 catgtgaacg ctacttatgt gaacgtaaaa tgtgtcgctc cgtatccttc tctgttgtca 2820 tcagaagata acgctgatga tgaggtggac acacgaccag cctccttctg ggagacatca 2880 tag 2883 <210> 35 <211> 7650 <212> DNA <213> Artificial Sequence <220> <223> MTOR <400> 35 atgcttggaa ccggacctgc cgccgccacc accgctgcca ccacatctag caatgtgagc 60 gtcctgcagc agtttgccag tggcctaaag agccggaatg aggaaaccag ggccaaagcc 120 gccaaggagc tccagcacta tgtcaccatg gaactccgag agatgagtca agaggagtct 180 actcgcttct atgaccaact gaaccatcac atttttgaat tggtttccag ctcagatgcc 240 aatgagagga aaggtggcat cttggccata gctagcctca taggagtgga aggtgggaat 300 gccacccgaa ttggcagatt tgccaactat cttcggaacc tcctcccctc caatgaccca 360 gttgtcatgg aaatggcatc caaggccatt ggccgtcttg ccatggcagg ggacactttt 420 accgctgagt acgtggaatt tgaggtgaag cgagccctgg aatggctggg tgctgaccgc 480 aatgagggcc ggagacatgc agctgtcctg gttctccgtg agctggccat cagcgtccct 540 accttcttct tccagcaagt gcaacccttc tttgacaaca tttttgtggc cgtgtgggac 600 cccaaacagg ccatccgtga gggagctgta gccgcccttc gtgcctgtct gattctcaca 660 acccagcgtg agccgaagga gatgcagaag cctcagtggt acaggcacac atttgaagaa 720 gcagagaagg gatttgatga gaccttggcc aaagagaagg gcatgaatcg ggatgatcgg 780 atccatggag ccttgttgat ccttaacgag ctggtccgaa tcagcagcat ggagggagag 840 cgtctgagag aagaaatgga agaaatcaca cagcagcagc tggtacacga caagtactgc 900 aaagatctca tgggcttcgg aacaaaacct cgtcacatta cccccttcac cagtttccag 960 gctgtacagc cccagcagtc aaatgccttg gtggggctgc tggggtacag ctctcaccaa 1020 ggcctcatgg gatttgggac ctcccccagt ccagctaagt ccaccctggt ggagagccgg 1080 tgttgcagag acttgatgga ggagaaattt gatcaggtgt gccagtgggt gctgaaatgc 1140 aggaatagca agaactcgct gatccaaatg acaatcctta atttgttgcc ccgcttggct 1200 gcattccgac cttctgcctt cacagatacc cagtatctcc aagataccat gaaccatgtc 1260 ctaagctgtg tcaagaagga gaaggaacgt acagcggcct tccaagccct ggggctactt 1320 tctgtggctg tgaggtctga gtttaaggtc tatttgcctc gcgtgctgga catcatccga 1380 gcggccctgc ccccaaagga cttcgcccat aagaggcaga aggcaatgca ggtggatgcc 1440 acagtcttca cttgcatcag catgctggct cgagcaatgg ggccaggcat ccagcaggat 1500 atcaaggagc tgctggagcc catgctggca gtgggactaa gccctgccct cactgcagtg 1560 ctctacgacc tgagccgtca gattccacag ctaaagaagg acatcaaga tgggctactg 1620 aaaatgctgt ccctggtcct tatgcacaaa ccccttcgcc acccaggcat gcccaagggc 1680 ctggcccatc agctggcctc tcctggcctc acgaccctcc ctgaggccag cgatgtgggc 1740 agcatcactc ttgccctccg aacgcttggc agctttgaat ttgaaggcca ctctctgacc 1800 caatttgttc gccactgtgc ggatcatttc ctgaacagtg agcacaagga gatccgcatg 1860 gaggctgccc gcacctgctc ccgcctgctc acaccctcca tccacctcat cagtggccat 1920 gctcatgtgg ttagccagac cgcagtgcaa gtggtggcag atgtgcttag caaactgctc 1980 gtagttggga taacagatcc tgaccctgac attcgctact gtgtcttggc gtccctggac 2040 gagcgctttg atgcacacct ggcccaggcg gagaacttgc aggccttgtt tgtggctctg 2100 aatgaccagg tgtttgagat ccgggagctg gccatctgca ctgtgggccg actcagtagc 2160 atgaaccctg cctttgtcat gcctttcctg cgcaagatgc tcatccagat tttgacagag 2220 ttggagcaca gtgggattgg aagaatcaaa gagcagagtg cccgcatgct ggggcacctg 2280 gtctccaatg ccccccgact catccgcccc tacatggagc ctattctgaa ggcattaatt 2340 ttgaaactga aagatccaga ccctgatcca aacccaggtg tgatcaataa tgtcctggca 2400 acaataggag aattggcaca ggttagtggc ctggaaatga ggaaatgggt tgatgaactt 2460 tttattatca tcatggacat gctccaggat tcctctttgt tggccaaaag gcaggtggct 2520 ctgtggaccc tgggacagtt ggtggccagc actggctatg tagtagagcc ctacaggaag 2580 taccctactt tgcttgaggt gctactgaat tttctgaaga ctgagcagaa ccagggtaca 2640 cgcagagagg ccatccgtgt gttagggctt ttaggggctt tggatcctta caagcacaaa 2700 gtgaacattg gcatgataga ccagtcccgg gatgcctctg ctgtcagcct gtcagaatcc 2760 aagtcaagtc aggattcctc tgactatagc actagtgaaa tgctggtcaa catgggaaac 2820 ttgcctctgg atgagttcta cccagctgtg tccatggtgg ccctgatgcg gatcttccga 2880 gaccagtcac tctctcatca tcacaccatg gttgtccagg ccatcacctt catcttcaag 2940 tccctgggac tcaaatgtgt gcagttcctg ccccaggtca tgccccacgtt ccttaacgtc 3000 attcgagtct gtgatggggc catccgggaa tttttgttcc agcagctggg aatgttggtg 3060 tcctttgtga agagccacat cagaccttat atggatgaaa tagtcaccct catgagagaa 3120 ttctgggtca tgaacacctc aattcagagc acgatcattc ttctcattga gcaaattgtg 3180 gtagctcttg ggggtgaatt taagctctac ctgccccagc tgatcccaca catgctgcgt 3240 gtcttcatgc atgacaacag cccaggccgc attgtctcta tcaagttact ggctgcaatc 3300 cagctgtttg gcgccaacct ggatgactac ctgcatttac tgctgcctcc tattgttaag 3360 ttgtttgatg cccctgaagc tccactgcca tctcgaaagg cagcgctaga gactgtggac 3420 cgcctgacgg agtccctgga tttcactgac tatgcctccc ggatcattca ccctattgtt 3480 cgaacactgg accagagccc agaactgcgc tccacagcca tggacacgct gtcttcactt 3540 gtttttcagc tggggaagaa gtaccaaatt ttcattccaa tggtgaataa agttctggtg 3600 cgacaccgaa tcaatcatca gcgctatgat gtgctcatct gcagaattgt caagggatac 3660 acacttgctg atgaagagga ggatcctttg atttaccagc atcggatgct taggagtggc 3720 caaggggatg cattggctag tggaccagtg gaaacaggac ccatgaagaa actgcacgtc 3780 agcaccatca acctccaaaa ggcctggggc gctgccagga gggtctccaa agatgactgg 3840 ctggaatggc tgagacggct gagcctggag ctgctgaagg actcatcatc gccctccctg 3900 cgctcctgct gggccctggc acaggcctac aacccgatgg ccagggatct cttcaatgct 3960 gcatttgtgt cctgctggtc tgaactgaat gaagatcaac aggatgagct catcagaagc 4020 atcgagttgg ccctcacctc acaagacatc gctgaagtca cacagaccct cttaaacttg 4080 gctgaattca tggaacacag tgacaagggc cccctgccac tgagagatga caatggcatt 4140 gttctgctgg gtgagagagc tgccaagtgc cgagcatatg ccaaagcact acactacaaa 4200 gaactggagt tccagaaagg ccccacccct gccattctag aatctctcat cagcattaat 4260 aataagctac agcagccgga ggcagcggcc ggagtgttag aatatgccat gaaacacttt 4320 ggagagctgg agatccaggc tacctggtat gagaaactgc acgagtggga ggatgccctt 4380 gtggcctatg acaagaaaat ggacaccaac aaggacgacc cagagctgat gctgggccgc 4440 atgcgctgcc tcgaggcctt gggggaatgg ggtcaactcc accagcagtg ctgtgaaaag 4500 tggaccctgg ttaatgatga gacccaagcc aagatggccc ggatggctgc tgcagctgca 4560 tggggtttag gtcagtggga cagcatggaa gaatacacct gtatgatccc tcgggacacc 4620 catgatgggg cattttatag agctgtgctg gcactgcatc aggacctctt ctccttggca 4680 caacagtgca ttgacaaggc cagggacctg ctggatgctg aattaactgc gatggcagga 4740 gagagttaca gtcgggcata tggggccatg gtttcttgcc acatgctgtc cgagctggag 4800 gaggttatcc agtacaaact tgtccccgag cgacgagaga tcatccgcca gatctggtgg 4860 gagagactgc agggctgcca gcgtatcgta gaggactggc agaaaatcct tatggtgcgg 4920 tcccttgtgg tcagccctca tgaagacatg agaacctggc tcaagtatgc aagcctgtgc 4980 ggcaagagtg gcaggctggc tcttgctcat aaaactttag tgttgctcct gggagttgat 5040 ccgtctcggc aacttgacca tcctctgcca acagttcacc ctcaggtgac ctatgcctac 5100 atgaaaaaca tgtggaagag tgcccgcaag atcgatgcct tccagcacat gcagcatttt 5160 gtccagacca tgcagcaaca ggcccagcat gccatcgcta ctgaggacca gcagcataag 5220 caggaactgc acaagctcat ggcccgatgc ttcctgaaac ttggagagtg gcagctgaat 5280 ctacagggca tcaatgagag cacaatcccc aaagtgctgc agtactacag cgccgccaca 5340 gagcacgacc gcagctggta caaggcctgg catgcgtggg cagtgatgaa cttcgaagct 5400 gtgctacact acaaacatca gaaccaagcc cgcgatgaga agaagaaact gcgtcatgcc 5460 agcggggcca acatcaccaa cgccaccact gccgccacca cggccgccac tgccaccacc 5520 actgccagca ccgagggcag caacagtgag agcgaggccg agagcaccga gaacagcccc 5580 accccatcgc cgctgcagaa gaaggtcact gaggatctgt ccaaaaccct cctgatgtac 5640 acggtgcctg ccgtccaggg cttcttccgt tccatctcct tgtcacgagg caacaacctc 5700 caggatacac tcagagttct caccttatgg tttgattatg gtcactggcc agatgtcaat 5760 gaggccttag tggagggggt gaaagccatc cagattgata cctggctaca ggttatacct 5820 cagctcattg caagaattga tacgcccaga cccttggtgg gacgtctcat tcaccagctt 5880 ctcacagaca ttggtcggta ccacccccag gccctcatct acccactgac agtggcttct 5940 aagtctacca cgacagcccg gcacaatgca gccaacaaga ttctgaagaa catgtgtgag 6000 cacagcaaca ccctggtcca gcaggccatg atggtgagcg aggagctgat ccgagtggcc 6060 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg 6120 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg 6180 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag 6240 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cacccaagcc 6300 tgggacctct attatcatgt gttccgacga atctcaaagc agctgcctca gctcacatcc 6360 ttagagctgc aatatgtttc cccaaaactt ctgatgtgcc gggaccttga attggctgtg 6420 ccaggaacat atgaccccaa ccagccaatc attcgcattc agtccatagc accgtctttg 6480 caagtcatca catccaagca gaggccccgg aaattgacac ttatgggcag caacggacat 6540 gagtttgttt tccttctaaa aggccatgaa gatctgcgcc aggatgagcg tgtgatgcag 6600 ctcttcggcc tggttaacac ccttctggcc aatgacccaa catctcttcg gaaaaacctc 6660 agcatccaga gatacgctgt catcccttta tcgaccaact cgggcctcat tggctgggtt 6720 ccccactgtg acacactgca cgccctcatc cgggactaca gggagaagaa gaagatcctt 6780 ctcaacatcg agcatcgcat catgttgcgg atggctccgg actatgacca cttgactctg 6840 atgcagaagg tggaggtgtt tgagcatgcc gtcaataata cagctgggga cgacctggcc 6900 aagctgctgt ggctgaaaag ccccagctcc gaggtgtggt ttgaccgaag aaccaattat 6960 acccgttctt tagcggtcat gtcaatggtt gggtatattt taggcctggg agatagacac 7020 ccatccaacc tgatgctgga ccgtctgagt gggaagatcc tgcacattga ctttggggac 7080 tgctttgagg ttgctatgac ccgagagaag tttccagaga agattccatt tagactaaca 7140 agaatgttga ccaatgctat ggaggttaca ggcctggatg gcaactacag aatcacatgc 7200 cacacagtga tggaggtgct gcgagagcac aaggacagtg tcatggccgt gctggaagcc 7260 tttgtctatg accccttgct gaactggagg ctgatggaca caaataccaa aggcaacaag 7320 cgatcccgaa cgaggacgga ttcctactct gctggccagt cagtcgaaat tttggacggt 7380 gtggaacttg gagagccagc ccataagaaa acggggacca cagtgccaga atctattcat 7440 tctttcattg gagacggttt ggtgaaacca gaggccctaa ataagaaagc tatccagatt 7500 attaacaggg ttcgagataa gctcactggt cgggacttct ctcatgatga cactttggat 7560 gttccaacgc aagttgagct gctcatcaaa caagcgacat cccatgaaaa cctctgccag 7620 tgctatattg gctggtgccc tttctggtaa 7650 <210> 36 <211> 762 <212> DNA <213> Artificial Sequence <220> <223> MYCN <400> 36 atgcgggggg ctcctgggaa ctgtgttgga gccgagcaag cgctagccag gcgcaagcgc 60 gcacagactg tagccatccg aggacacccc cgccccccg gcccacccgg agacacccgc 120 gcagaatcgc ctccggatcc cctgcagtcg gcgggagatg atgaagatga tgaagaggaa 180 gatgaagagg aagaaatcga cgtggtcact gtggagaagc ggcgttcctc ctccaacacc 240 aaggctgtca ccacattcac catcactgtg cgtcccaaga acgcagccct gggtcccggg 300 agggctcagt ccagcgagct gatcctcaaa cgatgccttc ccatccacca gcagcacaac 360 tatgccgccc cctctcccta cgtggagagt gaggatgcac ccccacagaa gaagataaag 420 agcgaggcgt ccccacgtcc gctcaagagt gtcatccccc caaaggctaa gagcttgagc 480 ccccgaaact ctgactcgga ggacagtgag cgtcgcagaa accacaacat cctggagcgc 540 cagcgccgca acgaccttcg gtccagcttt ctcacgctca gggaccacgt gccggagttg 600 gtaaagaatg agaaggccgc caaggtggtc attttgaaaa aggccactga gtatgtccac 660 tccctccagg ccgaggagca ccagcttttg ctggaaaagg aaaaattgca ggcaagacag 720 cagcagttgc taaagaaaat tgaacacgct cggacttgct ag 762 <210> 37 <211> 7668 <212> DNA <213> Artificial Sequence <220> <223> NOTCH1 <400> 37 atgccgccgc tcctggcgcc cctgctctgc ctggcgctgc tgcccgcgct cgccgcacga 60 ggcccgcgat gctcccagcc cggtgagacc tgcctgaatg gcgggaagtg tgaagcggcc 120 aatggcacgg aggcctgcgt ctgtggcggg gccttcgtgg gcccgcgatg ccaggacccc 180 aacccgtgcc tcagcacccc ctgcaagaac gccgggacat gccacgtggt ggaccgcaga 240 ggcgtggcag actatgcctg cagctgtgcc ctgggcttct ctgggcccct ctgcctgaca 300 cccctggaca atgcctgcct caccaacccc tgccgcaacg ggggcacctg cgacctgctc 360 acgctgacgg agtacaagtg ccgctgcccg cccggctggt cagggaaatc gtgccagcag 420 gctgacccgt gcgcctccaa cccctgcgcc aacggtggcc agtgcctgcc cttcgaggcc 480 tcctacatct gccactgccc acccagcttc catggcccca cctgccggca ggatgtcaac 540 gagtgtggcc agaagcccgg gctttgccgc cacggaggca cctgccacaa cgaggtcggc 600 tcctaccgct gcgtctgccg cgccacccac actggcccca actgcgagcg gccctacgtg 660 ccctgcagcc cctcgccctg ccagaacggg ggcacctgcc gccccacggg cgacgtcacc 720 cacgagtgtg cctgcctgcc aggcttcacc ggccagaact gtgaggaaaa tatcgacgat 780 tgtccaggaa acaactgcaa gaacgggggt gcctgtgtgg acggcgtgaa cacctacaac 840 tgccgctgcc cgccagagtg gacaggtcag tactgtaccg aggatgtgga cgagtgccag 900 ctgatgccaa atgcctgcca gaacggcggg acctgccaca acacccacgg tggctacaac 960 tgcgtgtgtg tcaacggctg gactggtgag gactgcagcg agaacattga tgactgtgcc 1020 agcgccgcct gcttccacgg cgccacctgc catgaccgtg tggcctcctt ctactgcgag 1080 tgtccccatg gccgcacagg tctgctgtgc cacctcaacg acgcatgcat cagcaacccc 1140 tgtaacgagg gctccaactg cgacaccaac cctgtcaatg gcaaggccat ctgcacctgc 1200 ccctcggggt acaggggccc ggcctgcagc caggacgtgg atgagtgctc gctgggtgcc 1260 aacccctgcg agcatgcggg caagtgcatc aacacgctgg gctccttcga gtgccagtgt 1320 ctgcagggct acacgggccc ccgatgcgag atcgacgtca acgagtgcgt ctcgaacccg 1380 tgccagaacg acgccacctg cctggaccag attggggagt tccagtgcat ctgcatgccc 1440 ggctacgagg gtgtgcactg cgaggtcaac acagacgagt gtgccagcag cccctgcctg 1500 cacaatggcc gctgcctgga caagatcaat gagttccagt gcgagtgccc cacgggcttc 1560 actgggcatc tgtgccagta cgatgtggac gagtgtgcca gcaccccctg caagaatggt 1620 gccaagtgcc tggacggacc caacacttac acctgtgtgt gcacggaagg gtacacgggg 1680 acgcactgcg aggtggacat cgatgagtgc gaccccgacc cctgccacta cggctcctgc 1740 aaggacggcg tcgccacctt cacctgcctc tgccgcccag gctacacggg ccaccactgc 1800 gagaccaaca tcaacgagtg ctccagccag ccctgccgcc acgggggcac ctgccaggac 1860 cgcgacaacg cctacctctg cttctgcctg aaggggacca caggacccaa ctgcgagatc 1920 aacctggatg actgtgccag cagcccctgc gactcgggca cctgtctgga caagatcgat 1980 ggctacgagt gtgcctgtga gccgggctac acagggagca tgtgtaacat caacatcgat 2040 gagtgtgcgg gcaacccctg ccacaacggg ggcacctgcg aggacggcat caatggcttc 2100 acctgccgct gccccgaggg ctaccacgac cccacctgcc tgtctgaggt caatgagtgc 2160 aacagcaacc cctgcgtcca cggggcctgc cgggacagcc tcaacgggta caagtgcgac 2220 tgtgaccctg ggtggagtgg gaccaactgt gacatcaaca acaatgagtg tgaatccaac 2280 ccttgtgtca acggcggcac ctgcaaagac atgaccagtg gctacgtgtg cacctgccgg 2340 gagggcttca gcggtcccaa ctgccagacc aacatcaacg agtgtgcgtc caacccatgt 2400 ctgaaccagg gcacgtgtat tgacgacgtt gccgggtaca agtgcaactg cctgctgccc 2460 tacacaggtg ccacgtgtga ggtggtgctg gccccgtgtg cccccagccc ctgcagaaac 2520 ggcggggagt gcaggcaatc cgaggactat gagagcttct cctgtgtctg ccccacgggc 2580 tggcaagggc agacctgtga ggtcgacatc aacgagtgcg ttctgagccc gtgccggcac 2640 ggcgcatcct gccagaacac ccacggcggc taccgctgcc actgccaggc cggctacagt 2700 gggcgcaact gcgagaccga catcgacgac tgccggccca acccgtgtca caacgggggc 2760 tcctgcacag acggcatcaa cacggccttc tgcgactgcc tgcccggctt ccggggcact 2820 ttctgtgagg aggacatcaa cgagtgtgcc agtgacccct gccgcaacgg ggccaactgc 2880 acggactgcg tggacagcta cacgtgcacc tgccccgcag gcttcagcgg gatccactgt 2940 gagaacaaca cgcctgactg cacagagagc tcctgcttca acggtggcac ctgcgtggac 3000 ggcatcaact cgttcacctg cctgtgtcca cccggcttca cgggcagcta ctgccagcac 3060 gatgtcaatg agtgcgactc acagccctgc ctgcatggcg gcacctgtca ggacggctgc 3120 ggctcctaca ggtgcacctg cccccagggc tacactggcc ccaactgcca gaaccttgtg 3180 cactggtgtg actcctcgcc ctgcaagaac ggcggcaaat gctggcagac ccacacccag 3240 taccgctgcg agtgccccag cggctggacc ggcctttact gcgacgtgcc cagcgtgtcc 3300 tgtgaggtgg ctgcgcagcg acaaggtgtt gacgttgccc gcctgtgcca gcatggaggg 3360 ctctgtgtgg acgcgggcaa cacgcaccac tgccgctgcc aggcgggcta cacaggcagc 3420 tactgtgagg acctggtgga cgagtgctca cccagcccct gccagaacgg ggccacctgc 3480 acggactacc tgggcggcta ctcctgcaag tgcgtggccg gctaccacgg ggtgaactgc 3540 tctgaggaga tcgacgagtg cctctcccac ccctgccaga acgggggcac ctgcctcgac 3600 ctccccaaca cctacaagtg ctcctgccca cggggcactc agggtgtgca ctgtgagatc 3660 aacgtggacg actgcaatcc ccccgttgac cccgtgtccc ggagccccaa gtgctttaac 3720 aacggcacct gcgtggacca ggtgggcggc tacagctgca cctgcccgcc gggcttcgtg 3780 ggtgagcgct gtgaggggga tgtcaacgag tgcctgtcca atccctgcga cgcccgtggc 3840 acccagaact gcgtgcagcg cgtcaatgac ttccactgcg agtgccgtgc tggtcacacc 3900 gggcgccgct gcgagtccgt catcaatggc tgcaaaggca agccctgcaa gaatgggggc 3960 acctgcgccg tggcctccaa caccgcccgc gggttcatct gcaagtgccc tgcgggcttc 4020 gagggcgcca cgtgtgagaa tgacgctcgt acctgcggca gcctgcgctg cctcaacggc 4080 ggcacatgca tctccggccc gcgcagcccc acctgcctgt gcctgggccc cttcaggggc 4140 cccgaatgcc agttcccggc cagcagcccc tgcctgggcg gcaacccctg ctacaaccag 4200 gggacctgtg agcccacatc cgagagcccc ttctaccgtt gcctgtgccc cgccaaattc 4260 aacgggctct tgtgccacat cctggactac agcttcgggg gtggggccgg gcgcgacatc 4320 cccccgccgc tgatcgagga ggcgtgcgag ctgcccgagt gccaggagga cgcgggcaac 4380 aaggtctgca gcctgcagtg caacaaccac gcgtgcggct gggacggcgg tgactgctcc 4440 ctcaacttca atgacccctg gaagaactgc acgcagtctc tgcagtgctg gaagtacttc 4500 agtgacggcc actgtgacag ccagtgcaac tcagccggct gcctcttcga cggctttgac 4560 tgccagcgtg cggaaggcca gtgcaacccc ctgtacgacc agtactgcaa ggaccacttc 4620 agcgacgggc actgcgacca gggctgcaac agcgcggagt gcgagtggga cgggctggac 4680 tgtgcggagc atgtacccga gaggctggcg gccggcacgc tggtggtggt ggtgctgatg 4740 ccgccggagc agctgcgcaa cagctccttc cacttcctgc gggagctcag ccgcgtgctg 4800 cacaccaacg tggtcttcaa gcgtgacgca cacggccagc agatgatctt cccctactac 4860 ggccgcgagg aggagctgcg caagcacccc atcaagcgtg ccgccgaggg ctgggccgca 4920 cctgacgccc tgctgggcca ggtgaaggcc tcgctgctcc ctggtggcag cgagggtggg 4980 cggcggcgga gggagctgga ccccatggac gtccgcggct ccatcgtcta cctggagatt 5040 gacaaccggc agtgtgtgca ggcctcctcg cagtgcttcc agagtgccac cgacgtggcc 5100 gcattcctgg gagcgctcgc ctcgctgggc agcctcaaca tcccctacaa gatcgaggcc 5160 gtgcagagtg agaccgtgga gccgcccccg ccggcgcagc tgcacttcat gtacgtggcg 5220 gcggccgcct ttgtgcttct gttcttcgtg ggctgcgggg tgctgctgtc ccgcaagcgc 5280 cggcggcagc atggccagct ctggttccct gagggcttca aagtgtctga ggccagcaag 5340 aagaagcggc gggagcccct cggcgaggac tccgtgggcc tcaagcccct gaagaacgct 5400 tcagacggtg ccctcatgga cgacaaccag aatgagtggg gggacgagga cctggagacc 5460 aagaagttcc ggttcgagga gcccgtggtt ctgcctgacc tggacgacca gacagaccac 5520 cggcagtgga ctcagcagca cctggatgcc gctgacctgc gcatgtctgc catggccccc 5580 acaccgcccc agggtgaggt tgacgccgac tgcatggacg tcaatgtccg cgggcctgat 5640 ggcttcaccc cgctcatgat cgcctcctgc agcggggggcg gcctggagac gggcaacagc 5700 gaggaagagg aggacgcgcc ggccgtcatc tccgacttca tctaccaggg cgccagcctg 5760 cacaaccaga cagaccgcac gggcgagacc gccttgcacc tggccgcccg ctactcacgc 5820 tctgatgccg ccaagcgcct gctggaggcc agcgcagatg ccaacatcca ggacaacatg 5880 ggccgcaccc cgctgcatgc ggctgtgtct gccgacgcac aaggtgtctt ccagatcctg 5940 atccggaacc gagccacaga cctggatgcc cgcatgcatg atggcacgac gccactgatc 6000 ctggctgccc gcctggccgt ggagggcatg ctggaggacc tcatcaactc acacgccgac 6060 gtcaacgccg tagatgacct gggcaagtcc gccctgcact gggccgccgc cgtgaacaat 6120 gtggatgccg cagttgtgct cctgaagaac ggggctaaca aagatatgca gaacaacagg 6180 gaggagacac ccctgtttct ggccgcccgg gagggcagct acgagaccgc caaggtgctg 6240 ctggaccact ttgccaaccg ggacatcacg gatcatatgg accgcctgcc gcgcgacatc 6300 gcacaggagc gcatgcatca cgacatcgtg aggctgctgg acgagtacaa cctggtgcgc 6360 agcccgcagc tgcacggagc cccgctgggg ggcacgccca ccctgtcgcc cccgctctgc 6420 tcgcccaacg gctacctggg cagcctcaag cccggcgtgc agggcaagaa ggtccgcaag 6480 cccagcagca aaggcctggc ctgtggaagc aaggaggcca aggacctcaa ggcacggagg 6540 aagaagtccc aggacggcaa gggctgcctg ctggacagct ccggcatgct ctcgcccgtg 6600 gactccctgg agtcacccca tggctacctg tcagacgtgg cctcgccgcc actgctgccc 6660 tccccgttcc agcagtctcc gtccgtgccc ctcaaccacc tgcctgggat gcccgacacc 6720 cacctgggca tcgggcacct gaacgtggcg gccaagcccg agatggcggc gctgggtggg 6780 ggcggccggc tggcctttga gactggccca cctcgtctct cccacctgcc tgtggcctct 6840 ggcaccagca ccgtcctggg ctccagcagc ggaggggccc tgaatttcac tgtgggcggg 6900 tccaccagtt tgaatggtca atgcgagtgg ctgtcccggc tgcagagcgg catggtgccg 6960 aaccaataca accctctgcg ggggagtgtg gcaccaggcc ccctgagcac acaggccccc 7020 tccctgcagc atggcatggt aggcccgctg cacagtagcc ttgctgccag cgccctgtcc 7080 cagatgatga gctaccaggg cctgcccagc acccggctgg ccacccagcc tcacctggtg 7140 cagacccagc aggtgcagcc acaaaactta cagatgcagc agcagaacct gcagccagca 7200 aacatccagc agcagcaaag cctgcagccg ccaccaccac caccacagcc gcaccttggc 7260 gtgagctcag cagccagcgg ccacctgggc cggagcttcc tgagtggaga gccgagccag 7320 gcagacgtgc agccactggg ccccagcagc ctggcggtgc acactattct gccccaggag 7380 agccccgccc tgccccacgtc gctgccatcc tcgctggtcc cacccgtgac cgcagcccag 7440 ttcctgacgc ccccctcgca gcacagctac tcctcgcctg tggacaacac ccccagccac 7500 cagctacagg tgcctgagca ccccttcctc accccgtccc ctgagtcccc tgaccagtgg 7560 tccagctcgt ccccgcattc caacgtctcc gactggtccg agggcgtctc cagccctccc 7620 accagcatgc agtcccagat cgcccgcatt ccggaggcct tcaagtaa 7668 <210> 38 <211> 570 <212> DNA <213> Artificial Sequence <220> <223> NRAS <400> 38 atgactgagt acaaactggt ggtggttgga gcaggtggtg ttgggaaaag cgcactgaca 60 atccagctaa tccagaacca ctttgtagat gaatatgatc ccaccataga ggattcttac 120 agaaaacaag tggttataga tggtgaaacc tgtttgttgg acatactgga tacagctgga 180 caagaagagt acagtgccat gagagaccaa tacatgagga caggcgaagg cttcctctgt 240 gtatttgcca tcaataatag caagtcattt gcggatatta acctctacag ggagcagatt 300 aagcgagtaa aagactcgga tgatgtacct atggtgctag tgggaaacaa gtgtgatttg 360 ccaacaagga cagttgatac aaaacaagcc cacgaactgg ccaagagtta cgggattcca 420 ttcattgaaa cctcagccaa gaccagacag ggtgttgaag atgcttttta cacactggta 480 agagaaatac gccagtaccg aatgaaaaaa ctcaacagca gtgatgatgg gactcagggt 540 tgtatgggat tgccatgtgt ggtgatgtaa 570 <210> 39 <211> 2424 <212> DNA <213> Artificial Sequence <220> <223> PDGFRA <400> 39 atggggactt cccatccggc gttcctggtc ttaggctgtc ttctcacagg gctgagccta 60 atcctctgcc agctttcatt accctctatc cttccaaatg aaaatgaaaa ggttgtgcag 120 ctgaattcat ccttttctct gagatgcttt ggggagagtg aagtgagctg gcagtacccc 180 atgtctgaag aagagagctc cgatgtggaa atcagaaatg aagaaaacaa cagcggcctt 240 tttgtgacgg tcttggaagt gagcagtgcc tcggcggccc acacagggtt gtacacttgc 300 tattacaacc acactcagac agaagagaat gagcttgaag gcaggcacat ttacatctat 360 gtgccagacc cagatgtagc ctttgtacct ctaggaatga cggattattt agtcatcgtg 420 gaggatgatg attctgccat tataccttgt cgcacaactg atcccgagac tcctgtaacc 480 ttacacaaca gtgagggggt ggtacctgcc tcctacgaca gcagacaggg ctttaatggg 540 accttcactg tagggcccta tatctgtgag gccaccgtca aaggaaagaa gttccagacc 600 atcccattta atgtttatgc tttaaaagca acatcagagc tggatctaga aatggaagct 660 cttaaaaccg tgtataagtc aggggaaacg attgtggtca cctgtgctgt ttttaacaat 720 gaggtggttg accttcaatg gacttaccct ggagaagtga aaggcaaagg catcacaatg 780 ctggaagaaa tcaaagtccc atccatcaaa ttggtgtaca ctttgacggt ccccgaggcc 840 acggtgaaag acagtggaga ttacgaatgt gctgcccgcc aggctaccag ggaggtcaaa 900 gaaatgaaga aagtcactat ttctgtccat gagaaaggtt tcattgaaat caaacccacc 960 ttcagccagt tggaagctgt caacctgcat gaagtcaaac attttgttgt agaggtgcgg 1020 gcctacccac ctcccaggat atcctggctg aaaaacaatc tgactctgat tgaaaatctc 1080 actgagatca ccactgatgt ggaaaagatt caggaaataa ggtatcgaag caaattaaag 1140 ctgatccgtg ctaaggaaga agacagtggc cattatacta ttgtagctca aaatgaagat 1200 gctgtgaaga gctatacttt tgaactgtta actcaagttc cttcatccat tctggacttg 1260 gtcgatgatc accatggctc aactggggga cagacggtga ggtgcacagc tgaaggcacg 1320 ccgcttcctg atattgagtg gatgatatgc aaagatatta agaaatgtaa taatgaaact 1380 tcctggacta ttttggccaa caatgtctca aacatcatca cggagatcca ctcccgagac 1440 aggagtaccg tggagggccg tgtgactttc gccaaagtgg aggagaccat cgccgtgcga 1500 tgcctggcta agaatctcct tggagctgag aaccgagagc tgaagctggt ggctcccacc 1560 ctgcgttctg aactcacggt ggctgctgca gtcctggtgc tgttggtgat tgtgatcatc 1620 tcacttattg tcctggttgt catttggaaa cagaaaccga ggtatgaaat tcgctggagg 1680 gtcattgaat caatcagccc agatggacat gaatatattt atgtggaccc gatgcagctg 1740 ccttatgact caagatggga gtttccaaga gatggactag tgcttggtcg ggtcttgggg 1800 tctggagcgt ttgggaaggt ggttgaagga acagcctatg gattaagccg gtcccaacct 1860 gtcatgaaag ttgcagtgaa gatgctaaaa cccacggcca gatccagtga aaaacaagct 1920 ctcatgtctg aactgaagat aatgactcac ctggggccac atttgaacat tgtaaacttg 1980 ctgggagcct gcaccaagtc aggccccatt tacatcatca cagagtattg cttctatgga 2040 gatttggtca actatttgca taagaatagg gatagcttcc tgagccacca cccagagaag 2100 ccaaagaaag agctggatat ctttggattg aaccctgctg atgaaagcac acggagctat 2160 gttattttat cttttgaaaa caatggtgac tacatggaca tgaagcaggc tgatactaca 2220 cagtatgtcc ccatgctaga aaggaaagag gtttctaaat attccgacat ccagagatca 2280 ctctatgatc gtccagcctc atataagaag aaatctatgt taggctgggc tggtggagtt 2340 ggcacgagat gtcagaggaa cctgagtcat gctcaggccc aagccctgtt ggcaggcaga 2400 ccactgcttt ctggccttcc gtga 2424 <210> 40 <211> 3207 <212> DNA <213> Artificial Sequence <220> <223> PIK3CA <400> 40 atgcctccac gaccatcatc aggtgaactg tggggcatcc acttgatgcc cccaagaatc 60 ctagtagaat gtttactacc aaatggaatg atagtgactt tagaatgcct ccgtgaggct 120 acattaataa ccataaagca tgaactattt aaagaagcaa gaaaataccc cctccatcaa 180 cttcttcaag atgaatcttc ttacattttc gtaagtgtta ctcaagaagc agaaagggaa 240 gaattttttg atgaaacaag acgactttgt gaccttcggc tttttcaacc ctttttaaaa 300 gtaattgaac cagtaggcaa ccgtgaagaa aagatcctca atcgagaaat tggttttgct 360 atcggcatgc cagtgtgtga atttgatatg gttaaagatc cagaagtaca ggacttccga 420 agaaatattc tgaacgtttg taaagaagct gtggatctta gggacctcaa ttcacctcat 480 agtagagcaa tgtatgtcta tcctccaaat gtagaatctt caccagaatt gccaaagcac 540 atatataata aattagataa agggcaaata atagtggtga tctgggtaat agtttctcca 600 aataatgaca agcagaagta tactctgaaa atcaaccatg actgtgtacc agaacaagta 660 attgctgaag caatcaggaa aaaaactcga agtatgttgc tatcctctga acaactaaaa 720 ctctgtgttt tagaatatca gggcaagtat attttaaaag tgtgtggatg tgatgaatac 780 ttcctagaaa aatatcctct gagtcagtat aagtatataa gaagctgtat aatgcttggg 840 aggatgccca atttgatgtt gatggctaaa gaaagccttt attctcaact gccaatggac 900 tgttttacaa tgccatctta ttccagacgc atttccacag ctacaccata tatgaatgga 960 gaaacatcta caaaatccct ttgggttata aatagtgcac tcagaataaa aattctttgt 1020 gcaacctacg tgaatgtaaa tattcgagac attgataaga tctatgttcg aacaggtatc 1080 taccatggag gagaaccctt atgtgacaat gtgaacactc aaagagtacc ttgttccaat 1140 cccaggtgga atgaatggct gaattatgat atatacattc ctgatcttcc tcgtgctgct 1200 cgactttgcc tttccatttg ctctgttaaa ggccgaaagg gtgctaaaga ggaacactgt 1260 ccattggcat ggggaaatat aaacttgttt gattacacag acactctagt atctggaaaa 1320 atggctttga atctttggcc agtacctcat ggattagaag atttgctgaa ccctattggt 1380 gttactggat caaatccaaa taaagaaact ccatgcttag agttggagtt tgactggttc 1440 agcagtgtgg taaagttccc agatatgtca gtgattgaag agcatgccaa ttggtctgta 1500 tcccgagaag caggatttag ctattcccac gcaggactga gtaacagact agctagagac 1560 aatgaattaa gggaaaatga caaagaacag ctcaaagcaa tttctacacg agatcctctc 1620 tctgaaatca ctgagcagga gaaagatttt ctatggagtc acagacacta ttgtgtaact 1680 atccccgaaa ttctacccaa attgcttctg tctgttaaat ggaattctag agatgaagta 1740 gcccagatgt attgcttggt aaaagattgg cctccaatca aacctgaaca ggctatggaa 1800 cttctggact gtaattaccc agatcctatg gttcgaggtt ttgctgttcg gtgcttggaa 1860 aaatatttaa cagatgacaa actttctcag tatttaattc agctagtaca ggtcctaaaa 1920 tatgaacaat atttggataa cttgcttgtg agatttttac tgaagaaagc attgactaat 1980 caaaggattg ggcacttttt cttttggcat ttaaaatctg agatgcacaa taaaacagtt 2040 agccagaggt ttggcctgct tttggagtcc tattgtcgtg catgtgggat gtatttgaag 2100 cacctgaata ggcaagtcga ggcaatggaa aagctcatta acttaactga cattctcaaa 2160 caggagaaga aggatgaaac acaaaaggta cagatgaagt ttttagttga gcaaatgagg 2220 cgaccagatt tcatggatgc tctacagggc tttctgtctc ctctaaaccc tgctcatcaa 2280 ctaggaaacc tcaggcttga agagtgtcga attatgtcct ctgcaaaaag gccactgtgg 2340 ttgaattggg agaacccaga catcatgtca gagttactgt ttcagaacaa tgagatcatc 2400 tttaaaaatg gggatgattt acggcaagat atgctaacac ttcaaattat tcgtattatg 2460 gaaaatatct ggcaaaatca aggtcttgat cttcgaatgt taccttatgg ttgtctgtca 2520 atcggtgact gtgtgggact tattgaggtg gtgcgaaatt ctcacactat tatgcaaatt 2580 cagtgcaaag gcggcttgaa aggtgcactg cagttcaaca gccacacact acatcagtgg 2640 ctcaaagaca agaacaaagg agaaatatat gatgcagcca ttgacctgtt tacacgttca 2700 tgtgctggat actgtgtagc taccttcatt ttgggaattg gagatcgtca caatagtaac 2760 atcatggtga aagacgatgg acaactgttt catatagatt ttggacactt tttggatcac 2820 aagaagaaaa aatttggtta taaacgagaa cgtgtgccat ttgttttgac acaggatttc 2880 ttaatagtga ttagtaaagg agcccaagaa tgcacaaaga caagagaatt tgagaggttt 2940 caggagatgt gttacaaggc ttatctagct attcgacagc atgccaatct cttcataaat 3000 cttttctcaa tgatgcttgg ctctggaatg ccagaactac aatcttttga tgacattgca 3060 tacattcgaa agaccctagc cttagataaa actgagcaag aggctttgga gtatttcatg 3120 aaacaaatga atgatgcaca tcatggtggc tggacaacaa aaatggattg gatcttccac 3180 acaattaaac agcatgcatt gaactga 3207 <210> 41 <211> 1434 <212> DNA <213> Artificial Sequence <220> <223> PPARG <400> 41 atgaccatgg ttgacacaga gatgccattc tggcccacca actttgggat cagctccgtg 60 gatctctccg taatggaaga ccactcccac tcctttgata tcaagccctt cactactgtt 120 gacttctcca gcatttctac tccacattac gaagacattc cattcacaag aacagatcca 180 gtggttgcag attacaagta tgacctgaaa cttcaagagt accaaagtgc aatcaaagtg 240 gagcctgcat ctccacctta ttattctgag aagactcagc tctacaataa gcctcatgaa 300 gagccttcca actccctcat ggcaattgaa tgtcgtgtct gtggagataa agcttctgga 360 tttcactatg gagttcatgc ttgtgaagga tgcaagggtt tcttccggag aacaatcaga 420 ttgaagctta tctatgacag atgtgatctt aactgtcgga tccacaaaaa aagtagaaat 480 aaatgtcagt actgtcggtt tcagaaatgc cttgcagtgg ggatgtctca taatgccatc 540 aggtttgggc ggatgccaca ggccgagaag gagaagctgt tggcggagat ctccagtgat 600 atcgaccagc tgaatccaga gtccgctgac ctccgggccc tggcaaaaca tttgtatgac 660 tcatacataa agtccttccc gctgaccaaa gcaaaggcga gggcgatctt gacaggaaag 720 acaacagaca aatcaccatt cgttatctat gacatgaatt ccttaatgat gggagaagat 780 aaaatcaagt tcaaacacat cacccccctg caggagcaga gcaaagaggt ggccatccgc 840 atctttcagg gctgccagtt tcgctccgtg gaggctgtgc aggagatcac agagtatgcc 900 aaaagcattc ctggttttgt aaatcttgac ttgaacgacc aagtaactct cctcaaatat 960 ggagtccacg agatcattta cacaatgctg gcctccttga tgaataaaga tggggttctc 1020 atatccgagg gccaaggctt catgacaagg gagtttctaa agagcctgcg aaagcctttt 1080 ggtgacttta tggagcccaa gtttgagttt gctgtgaagt tcaatgcact ggaattagat 1140 gacagcgact tggcaatatt tattgctgtc attattctca gtggagaccg cccaggtttg 1200 ctgaatgtga agcccattga agacattcaa gacaacctgc tacaagccct ggagctccag 1260 ctgaagctga accaccctga gtcctcacag ctgtttgcca agctgctcca gaaaatgaca 1320 gacctcagac agattgtcac ggaacacgtg cagctactgc aggtgatcaa gaagacggag 1380 acagacatga gtcttcaccc gctcctgcag gagatctaca aggacttgta ctag 1434 <210> 42 <211> 3891 <212> DNA <213> Artificial Sequence <220> <223> PTCH1 <400> 42 atgtttaatc ctcaactcat gatacagacc cctaaagaag aaggtgctaa tgtcctgacc 60 acagaagcgc tcctacaaca cctggactcg gcactccagg ccagccgtgt ccatgtatac 120 atgtacaaca ggcagtggaa attggaacat ttgtgttaca aatcaggaga gcttatcaca 180 gaaacaggtt acatggatca gataatagaa tatctttacc cttgtttgat tattacacct 240 ttggactgct tctgggaagg ggcgaaatta cagtctggga cagcatacct cctaggtaaa 300 cctcctttgc ggtggacaaa cttcgaccct ttggaattcc tggaagagtt aaagaaaata 360 aactatcaag tggacagctg ggaggaaatg ctgaataagg ctgaggttgg tcatggttac 420 atggaccgcc cctgcctcaa tccggccgat ccagactgcc ccgccacagc ccccaacaaa 480 aattcaacca aacctcttga tatggccctt gttttgaatg gtggatgtca tggcttatcc 540 agaaagtata tgcactggca ggaggagttg attgtgggtg gcacagtcaa gaacagcact 600 ggaaaactcg tcagcgccca tgccctgcag accatgttcc agttaatgac tcccaagcaa 660 atgtacgagc acttcaaggg gtacgagtat gtctcacaca tcaactggaa cgaggacaaa 720 gcggcagcca tcctggaggc ctggcagagg acatatgtgg aggtggttca tcagagtgtc 780 gcacagaact ccactcaaaa ggtgctttcc ttcaccacca cgaccctgga cgacatcctg 840 aaatccttct ctgacgtcag tgtcatccgc gtggccagcg gctacttact catgctcgcc 900 tatgcctgtc taaccatgct gcgctgggac tgctccaagt cccagggtgc cgtggggctg 960 gctggcgtcc tgctggttgc actgtcagtg gctgcaggac tgggcctgtg ctcattgatc 1020 ggaatttcct ttaacgctgc aacaactcag gttttgccat ttctcgctct tggtgttggt 1080 gtggatgatg tttttcttct ggcccacgcc ttcagtgaaa caggacagaa taaaagaatc 1140 ccttttgagg acaggaccgg ggagtgcctg aagcgcacag gagccagcgt ggccctcacg 1200 tccatcagca atgtcacagc cttcttcatg gccgcgttaa tcccaattcc cgctctgcgg 1260 gcgttctccc tccaggcagc ggtagtagtg gtgttcaatt ttgccatggt tctgctcatt 1320 tttcctgcaa ttctcagcat ggatttatat cgacgcgagg acaggagact ggatattttc 1380 tgctgtttta caagcccctg cgtcagcaga gtgattcagg ttgaacctca ggcctacacc 1440 gacacacacg acaatacccg ctacagcccc ccacctccct acagcagcca cagctttgcc 1500 catgaaacgc agattaccat gcagtccact gtccagctcc gcacggagta cgacccccac 1560 acgcacgtgt actacaccac cgctgagccg cgctccgaga tctctgtgca gcccgtcacc 1620 gtgacacagg acaccctcag ctgccagagc ccagagagca ccagctccac aagggacctg 1680 ctctcccagt tctccgactc cagcctccac tgcctcgagc ccccctgtac gaagtggaca 1740 ctctcatctt ttgctgagaa gcactatgct cctttcctct tgaaaccaaa agccaaggta 1800 gtggtgatct tcctttttct gggcttgctg ggggtcagcc tttatggcac cacccgagtg 1860 agagacgggc tggaccttac ggacattgta cctcgggaaa ccagagaata tgactttatt 1920 gctgcacaat tcaaatactt ttctttctac aacatgtata tagtcaccca gaaagcagac 1980 tacccgaata tccagcactt actttacgac ctacacagga gtttcagtaa cgtgaagtat 2040 gtcatgttgg aagaaaacaa acagcttccc aaaatgtggc tgcactactt cagagactgg 2100 cttcagggac ttcaggatgc atttgacagt gactgggaaa ccgggaaaat catgccaaac 2160 aattacaaga atggatcaga cgatggagtc cttgcctaca aactcctggt gcaaaccggc 2220 agccgcgata agcccatcga catcagccag ttgactaaac agcgtctggt ggatgcagat 2280 ggcatcatta atcccagcgc tttctacatc tacctgacgg cttgggtcag caacgacccc 2340 gtcgcgtatg ctgcctccca ggccaacatc cggccacacc gaccagaatg ggtccacgac 2400 aaagccgact acatgcctga aacaaggctg agaatcccgg cagcagagcc catcgagtat 2460 gcccagttcc ctttctacct caacggcttg cgggacacct cagactttgt ggaggcaatt 2520 gaaaaagtaa ggaccatctg cagcaactat acgagcctgg ggctgtccag ttacccccaac 2580 ggctacccct tcctcttctg ggagcagtac atcggcctcc gccactggct gctgctgttc 2640 atcagcgtgg tgttggcctg cacattcctc gtgtgcgctg tcttccttct gaacccctgg 2700 acggccggga tcattgtgat ggtcctggcg ctgatgacgg tcgagctgtt cggcatgatg 2760 ggcctcatcg gaatcaagct cagtgccgtg cccgtggtca tcctgatcgc ttctgttggc 2820 ataggagtgg agttcaccgt tcacgttgct ttggcctttc tgacggccat cggcgacaag 2880 aaccgcaggg ctgtgcttgc cctggagcac atgtttgcac ccgtcctgga tggcgccgtg 2940 tccactctgc tgggagtgct gatgctggcg ggatctgagt tcgacttcat tgtcaggtat 3000 ttctttgctg tgctggcgat cctcaccatc ctcggcgttc tcaatgggct ggttttgctt 3060 cccgtgcttt tgtctttctt tggaccatat cctgaggtgt ctccagccaa cggcttgaac 3120 cgcctgccca caccctcccc tgagccaccc cccagcgtgg tccgcttcgc catgccgccc 3180 ggccacacgc acagcgggtc tgattcctcc gactcggagt atagttccca gacgacagtg 3240 tcaggcctca gcgaggagct tcggcactac gaggcccagc agggcgcggg aggccctgcc 3300 caccaagtga tcgtggaagc cacagaaaac cccgtcttcg cccactccac tgtggtccat 3360 cccgaatcca ggcatcaccc accctcgaac ccgagacagc agccccacct ggactcaggg 3420 tccctgcctc ccggacggca aggccagcag ccccgcaggg acccccccag agaaggcttg 3480 tggccacccc cctacagacc gcgcagagac gcttttgaaa tttctactga agggcattct 3540 ggccctagca atagggcccg ctggggccct cgcggggccc gttctcacaa ccctcggaac 3600 ccagcgtcca ctgccatggg cagctccgtg cccggctact gccagcccat caccactgtg 3660 acggcttctg cctccgtgac tgtcgccgtg cacccgccgc ctgtccctgg gcctgggcgg 3720 aacccccgag ggggactctg cccaggctac cctgagactg accacggcct gtttgaggac 3780 ccccacgtgc ctttccacgt ccggtgtgag aggagggatt cgaaggtgga agtcattgag 3840 ctgcaggacg tggaatgcga ggagaggccc cggggaagca gctccaactg a 3891 <210> 43 <211> 621 <212> DNA <213> Artificial Sequence <220> <223> PTEN <400> 43 atgatgtttg aaactattcc aatgttcagt ggcggaactt gcaatcctca gtttgtggtc 60 tgccagctaa aggtgaagat atattcctcc aattcaggac ccacacgacg ggaagacaag 120 ttcatgtact ttgagttccc tcagccgtta cctgtgtgtg gtgatatcaa agtagagttc 180 ttccacaaac agaacaagat gctaaaaaag gacaaaatgt ttcacttttg ggtaaataca 240 ttcttcatac caggaccaga ggaaacctca gaaaaagtag aaaatggaag tctatgtgat 300 caagaaatcg atagcatttg cagtatagag cgtgcagata atgacaagga atatctagta 360 cttactttaa caaaaaatga tcttgacaaa gcaaataaag acaaagccaa ccgatacttt 420 tctccaaatt ttaaggtgaa gctgtacttc acaaaaacag tagaggagcc gtcaaatcca 480 gaggctagca gttcaacttc tgtaacacca gatgttagtg acaatgaacc tgatcattat 540 agatattctg acaccactga ctctgatcca gagaatgaac cttttgatga agatcagcat 600 acacaaatta caaaagtctg a 621 <210> 44 <211> 1947 <212> DNA <213> Artificial Sequence <220> <223> RAF1 <400> 44 atggagcaca tacagggagc ttggaagacg atcagcaatg gttttggatt caaagatgcc 60 gtgtttgatg gctccagctg catctctcct acaatagttc agcagtttgg ctatcagcgc 120 cgggcatcag atgatggcaa actcacagat ccttctaaga caagcaacac tatccgtgtt 180 ttcttgccga acaagcaaag aacagtggtc aatgtgcgaa atggaatgag cttgcatgac 240 tgccttatga aagcactcaa ggtgaggggc ctgcaaccag agtgctgtgc agtgttcaga 300 cttctccacg aacacaaagg taaaaaagca cgcttagatt ggaatactga tgctgcgtct 360 ttgattggag aagaacttca agtagatttc ctggatcatg ttcccctcac aacacacaac 420 tttgctcgga aaggttcct gaagcttgcc ttctgtgaca tctgtcagaa attcctgctc 480 aatggatttc gatgtcagac ttgtggctac aaatttcatg agcactgtag caccaaagta 540 cctactatgt gtgtggactg gagtaacatc agacaactct tattgtttcc aaattccact 600 attggtgata gtggagtccc agcactacct tctttgacta tgcgtcgtat gcgagagtct 660 gtttccagga tgcctgttag ttctcagcac agatattcta cacctcacgc cttcaccttt 720 aacacctcca gtccctcatc tgaaggttcc ctctcccaga ggcagaggtc gacatccaca 780 cctaatgtcc acatggtcag caccaccctg cctgtggaca gcaggatgat tgaggatgca 840 attcgaagtc acagcgaatc agcctcacct tcagccctgt ccagtagccc caacaatctg 900 agcccaacag gctggtcaca gccgaaaacc cccgtgccag cacaaagaga gcgggcacca 960 gtatctggga cccaggagaa aaacaaaatt aggcctcgtg gacagagaga ttcaagctat 1020 tattgggaaa tagaagccag tgaagtgatg ctgtccactc ggattgggtc aggctctttt 1080 ggaactgttt ataagggtaa atggcacgga gatgttgcag taaagatcct aaaggttgtc 1140 gacccaaccc cagagcaatt ccaggccttc aggaatgagg tggctgttct gcgcaaaaca 1200 cggcatgtga acattctgct tttcatgggg tacatgacaa aggacaacct ggcaattgtg 1260 acccagtggt gcgagggcag cagcctctac aaacacctgc atgtccagga gaccaagttt 1320 cagatgttcc agctaattga cattgcccgg cagacggctc agggaatgga ctatttgcat 1380 gcaaagaaca tcatccatag agacatgaaa tccaacaata tatttctcca tgaaggctta 1440 acagtgaaaa ttggagattt tggtttggca acagtaaagt cacgctggag tggttctcag 1500 caggttgaac aacctactgg ctctgtcctc tggatggccc cagaggtgat ccgaatgcag 1560 gataacaacc cattcagttt ccagtcggat gtctactcct atggcatcgt attgtatgaa 1620 ctgatgacgg gggagcttcc ttattctcac atcaacaacc gagatcagat catcttcatg 1680 gtgggccgag gatatgcctc cccagatctt agtaagctat ataagaactg ccccaaagca 1740 atgaagaggc tggtagctga ctgtgtgaag aaagtaaagg aagagaggcc tctttttccc 1800 cagatcctgt cttccattga gctgctccaa cactctctac cgaagatcaa ccggagcgct 1860 tccgagccat ccttgcatcg ggcagcccac actgaggata tcaatgcttg cacgctgacc 1920 acgtccccga ggctgcctgt cttctag 1947 <210> 45 <211> 3219 <212> DNA <213> Artificial Sequence <220> <223> RET <400> 45 atggcgaagg cgacgtccgg tgccgcgggg ctgcgtctgc tgttgctgct gctgctgccg 60 ctgctaggca aagtggcatt gggcctctac ttctcgaggg atgcttactg ggagaagctg 120 tatgtggacc aggcagccgg cacgcccttg ctgtacgtcc atgccctgcg ggacgcccct 180 gaggaggtgc ccagcttccg cctgggccag catctctacg gcacgtaccg cacacggctg 240 catgagaaca actggatctg catccaggag gacaccggcc tcctctacct taaccggagc 300 ctggaccata gctcctggga gaagctcagt gtccgcaacc gcggctttcc cctgctcacc 360 gtctacctca aggtcttcct gtcacccaca tcccttcgtg agggcgagtg ccagtggcca 420 ggctgtgccc gcgtatactt ctccttcttc aacacctcct ttccagcctg cagctccctc 480 aagccccggg agctctgctt cccagagaca aggccctcct tccgcattcg ggagaaccga 540 cccccaggca ccttccacca gttccgcctg ctgcctgtgc agttcttgtg ccccaacatc 600 agcgtggcct acaggctcct ggagggtgag ggtctgccct tccgctgcgc cccggacagc 660 ctggaggtga gcacgcgctg ggccctggac cgcgagcagc gggagaagta cgagctggtg 720 gccgtgtgca ccgtgcacgc cggcgcgcgc gaggaggtgg tgatggtgcc cttcccggtg 780 accgtgtacg acgaggacga ctcggcgccc accttccccg cgggcgtcga caccgccagc 840 gccgtggtgg agttcaagcg gaaggaggac accgtggtgg ccacgctgcg tgtcttcgat 900 gcagacgtgg tacctgcatc aggggagctg gtgaggcggt acacaagcac gctgctcccc 960 ggggacacct gggcccagca gaccttccgg gtggaacact ggcccaacga gacctcggtc 1020 caggccaacg gcagcttcgt gcgggcgacc gtacatgact ataggctggt tctcaaccgg 1080 aacctctcca tctcggagaa ccgcaccatg cagctggcgg tgctggtcaa tgactcagac 1140 ttccagggcc caggagcggg cgtcctcttg ctccacttca acgtgtcggt gctgccggtc 1200 agcctgcacc tgcccagtac ctactccctc tccgtgagca ggagggctcg ccgatttgcc 1260 cagatcggga aagtctgtgt ggaaaactgc caggcattca gtggcatcaa cgtccagtac 1320 aagctgcatt cctctggtgc caactgcagc acgctagggg tggtcacctc agccgaggac 1380 acctcgggga tcctgtttgt gaatgacacc aaggccctgc ggcggcccaa gtgtgccgaa 1440 cttcactaca tggtggtggc caccgaccag cagacctcta ggcaggccca ggcccagctg 1500 cttgtaacag tggaggggtc atatgtggcc gaggaggcgg gctgccccct gtcctgtgca 1560 gtcagcaaga gacggctgga gtgtgaggag tgtggcggcc tgggctcccc aacaggcagg 1620 tgtgagtgga ggcaaggaga tggcaaaggg atcaccagga acttctccac ctgctctccc 1680 agcaccaaga cctgccccga cggccactgc gatgttgtgg agacccaaga catcaacatt 1740 tgccctcagg actgcctccg gggcagcatt gttgggggac acgagcctgg ggagccccgg 1800 gggattaaag ctggctatgg cacctgcaac tgcttccctg aggaggagaa gtgcttctgc 1860 gagcccgaag acatccagga tccactgtgc gacgagctgt gccgcacggt gatcgcagcc 1920 gctgtcctct tctccttcat cgtctcggtg ctgctgtctg ccttctgcat ccactgctac 1980 cacaagtttg cccacaagcc acccatctcc tcagctgaga tgaccttccg gaggcccgcc 2040 caggccttcc cggtcagcta ctcctcttcc ggtgcccgcc ggccctcgct ggactccatg 2100 gagaaccagg tctccgtgga tgccttcaag atcctggagg atccaaagtg ggaattccct 2160 cggaagaact tggttcttgg aaaaactcta ggagaaggcg aatttggaaa agtggtcaag 2220 gcaacggcct tccatctgaa aggcagagca gggtacacca cggtggccgt gaagatgctg 2280 aaagagaacg cctccccgag tgagctgcga gacctgctgt cagagttcaa cgtcctgaag 2340 caggtcaacc acccacatgt catcaaattg tatggggcct gcagccagga tggcccgctc 2400 ctcctcatcg tggagtacgc caaatacggc tccctgcggg gcttcctccg cgagagccgc 2460 aaagtggggc ctggctacct gggcagtgga ggcagccgca actccagctc cctggaccac 2520 ccggatgagc gggccctcac catgggcgac ctcatctcat ttgcctggca gatctcacag 2580 gggatgcagt atctggccga gatgaagctc gttcatcggg acttggcagc cagaaacatc 2640 ctggtagctg aggggcggaa gatgaagatt tcggatttcg gcttgtcccg agatgtttat 2700 gaagaggatt cctacgtgaa gaggagccag ggtcggattc cagttaaatg gatggcaatt 2760 gaatcccttt ttgatcatat ctacaccacg caaagtgatg tatggtcttt tggtgtcctg 2820 ctgtgggaga tcgtgaccct agggggaaac ccctatcctg ggattcctcc tgagcggctc 2880 ttcaaccttc tgaagaccgg ccaccggatg gagaggccag acaactgcag cgaggagatg 2940 taccgcctga tgctgcaatg ctggaagcag gagccggaca aaaggccggt gtttgcggac 3000 atcagcaaag acctggagaa gatgatggtt aagaggagag actacttgga ccttgcggcg 3060 tccactccat ctgactccct gatttatgac gacggcctct cagaggagga gacaccgctg 3120 gtggactgta ataatgcccc cctccctcga gccctccctt ccacatggat tgaaaacaaa 3180 ctctatggta gaatttccca tgcatttact agattctag 3219 <210> 46 <211> 7044 <212> DNA <213> Artificial Sequence <220> <223> ROS1 <400> 46 atgaagaaca tttactgtct tattccgaag cttgtcaatt ttgcaactct tggctgccta 60 tggatttctg tggtgcagtg tacagtttta aatagctgcc taaagtcgtg tgtaactaat 120 ctgggccagc agcttgacct tggcacacca cataatctga gtgaaccgtg tatccaagga 180 tgtcactttt ggaactctgt agatcagaaa aactgtgctt taaagtgtcg ggagtcgtgt 240 gaggttggct gtagcagcgc ggaaggtgca tatgaagagg aagtactgga aaatgcagac 300 ctaccaactg ctccctttgc ttcttccatt ggaagccaca atatgacatt acgatggaaa 360 tctgcaaact tctctggagt aaaatacatc attcagtgga aatatgcaca acttctggga 420 agctggactt atactaagac tgtgtccaga ccgtcctatg tggtcaagcc cctgcacccc 480 ttcactgagt acattttccg agtggtttgg atcttcacag cgcagctgca gctctactcc 540 cctccaagtc ccagttacag gactcatcct catggagttc ctgaaactgc acctttgatt 600 aggaatattg agagctcaag tcccgacact gtggaagtca gctgggatcc acctcaattc 660 ccaggtggac ctattttggg ttataactta aggctgatca gcaaaaatca aaaattagat 720 gcagggacac agagaaccag tttccagttt tactccactt taccaaatac tatctacagg 780 ttttctattg cagcagtaaa tgaagttggt gagggtccag aagcagaatc tagtattacc 840 acttcatctt cagcagttca acaagaggaa cagtggctct ttttatccag aaaaacttct 900 ctaagaaaga gatctttaaa acatttagta gatgaagcac attgccttcg gttggatgct 960 atataccata atattacagg aatatctgtt gatgtccacc agcaaattgt ttatttctct 1020 gaaggaactc tcatatgggc gaagaaggct gccaacatgt ctgatgtatc tgacctgaga 1080 attttttaca gaggttcagg attaatttct tctatctcca tagattggct ttatcaaaga 1140 atgtatttca tcatggatga actggtatgt gtctgtgatt tagagaactg ctcaaacatc 1200 gaggaaatta ctccaccctc tattagtgca cctcaaaaaa ttgtggctga ttcatacaat 1260 gggtatgtct tttacctcct gagagatggc atttatagag cagaccttcc tgtaccatct 1320 ggccggtgtg cagaagctgt gcgtattgtg gagagttgca cgttaaagga ctttgcaatc 1380 aagccacaag ccaagcgaat catttacttc aatgacactg cccaagtctt catgtcaaca 1440 tttctggatg gctctgcttc ccatctcatc ctacctcgca tcccctttgc tgatgtgaaa 1500 agttttgctt gtgaaaacaa tgactttctt gtcacagatg gcaaggtcat tttccaacag 1560 gatgctttgt cttttaatga attcatcgtg ggatgtgacc tgagtcacat agaagaattt 1620 gggtttggta acttggtcat ctttggctca tcctcccagc tgcaccctct gccaggccgc 1680 ccgcaggagc tttcggtgct gtttggctct caccaggctc ttgttcaatg gaagcctcct 1740 gcccttgcca taggagccaa tgtcatcctg atcagtgata ttattgaact ctttgaatta 1800 ggcccttctg cctggcagaa ctggacctat gaggtgaaag tatccaccca agaccctcct 1860 gaagtcactc atattttctt gaacataagt ggaaccatgc tgaatgtacc tgagctgcag 1920 agtgctatga aatacaaggt ttctgtgaga gcaagttctc caaagaggcc aggcccctgg 1980 tcagagccct cagtgggtac taccctggtg ccagctagtg aaccaccatt tatcatggct 2040 gtgaaagaag atgggctttg gagtaaacca ttaaatagct ttggcccagg agagttctta 2100 tcctctgata taggaaatgt gtcagacatg gattggtata acaacagcct ctactacagt 2160 gacacgaaag gcgacgtttt tgtgtggctg ctgaatggga cggatatctc agagaattat 2220 cacctaccca gcattgcagg agcaggggct ttagcttttg agtggctggg tcactttctc 2280 tactgggctg gaaagacata tgtgatacaa aggcagtctg tgttgagggg acacacagac 2340 attgttaccc acgtgaagct attggtgaat gacatggtgg tggattcagt tggtggatat 2400 ctctactgga ccacactcta ttcagtggaa agcaccagac taaatgggga aagttccctt 2460 gtactacaga cacagccttg gttttctggg aaaaaggtaa ttgctctaac tttagacctc 2520 agtgatgggc tcctgtattg gttggttcaa gacagtcaat gtattcacct gtacacagct 2580 gttcttcggg gacagagcac tggggatacc accatcacag aatttgcagc ctggagtact 2640 tctgaaattt cccagaatgc actgatgtac tatagtggtc ggctgttctg gatcaatggc 2700 tttaggatta tcacaactca agaaataggt cagaaaacca gtgtctctgt tttggaacca 2760 gccagattta atcagttcac aattattcag acatccctta agcccctgcc agggaacttt 2820 tcctttaccc ctaaggttat tccagattct gttcaagagt cttcatttag gattgaagga 2880 aatgcttcaa gttttcaaat cctgtggaat ggtccccctg cggtagactg gggtgtagtt 2940 ttctacagtg tagaatttag tgctcattct aagttcttgg ctagtgaaca acactcttta 3000 cctgtattta ctgtggaagg actggaacct tatgccttat ttaatctttc tgtcactcct 3060 tatacctact ggggaaaggg ccccaaaaca tctctgtcac ttcgagcacc tgaaacagtt 3120 ccatcagcac cagagaaccc cagaatattt atattaccaa gtggaaaatg ctgcaacaag 3180 aatgaagttg tggtggaatt taggtggaac aaacctaagc atgaaaatgg ggtgttaaca 3240 aaatttgaaa ttttctacaa tatatccaat caaagtatta caaacaaaac atgtgaagac 3300 tggattgctg tcaatgtcac tccctcagtg atgtcttttc aacttgaagg catgagtccc 3360 agatgcttta ttgccttcca ggttagggcc tttacatcta aggggccagg accatatgct 3420 gacgttgtaa agtctacaac atcagaaatc aacccatttc ctcacctcat aactcttctt 3480 ggtaacaaga tagttttttt agatatggat caaaatcaag ttgtgtggac gttttcagca 3540 gaaagagtta tcagtgccgt ttgctacaca gctgataatg agatgggata ttatgctgaa 3600 ggggactcac tctttcttct gcacttgcac aatcgctcta gctctgagct tttccaagat 3660 tcactggttt ttgatatcac agttattaca attgactgga tttcaaggca cctctacttt 3720 gcactgaaag aatcacaaaa tggaatgcaa gtatttgatg ttgatcttga acacaaggtg 3780 aaatatccca gagaggtgaa gattcacaat aggaattcaa caataatttc tttttctgta 3840 tatcctcttt taagtcgctt gtattggaca gaagtttcca attttggcta ccagatgttc 3900 tactacagta ttatcagtca caccttgcac cgaattctgc aacccacagc tacaaaccaa 3960 caaaacaaaa ggaatcaatg ttcttgtaat gtgactgaat ttgagttaag tggagcaatg 4020 gctattgata cctctaacct agagaaacca ttgatatact ttgccaaagc acaagagatc 4080 tgggcaatgg atctggaagg ctgtcagtgt tggagagtta tcacagtacc tgctatgctc 4140 gcaggaaaaa cccttgttag cttaactgtg gatggagatc ttatatactg gatcatcaca 4200 gcaaaggaca gcacacagat ttatcaggca aagaaaggaa atggggccat cgtttcccag 4260 gtgaaggccc taaggagtag gcatatcttg gcttacagtt cagttatgca gccttttcca 4320 gataaagcgt ttctgtctct agcttcagac actgtggaac caactatact taatgccact 4380 aacactagcc tcacaatcag attacctctg gccaagacaa acctcacatg gtatggcatc 4440 accagcccta ctccaacata cctggtttat tatgcagaag ttaatgacag gaaaaacagc 4500 tctgacttga aatatagaat tctggaattt caggacagta tagctcttat tgaagattta 4560 caaccatttt caacatacat gatacagata gctgtaaaaa attattattc agatcctttg 4620 gaacatttac caccaggaaa agagatttgg ggaaaaacta aaaatggagt accagaggca 4680 gtgcagctca ttaatacaac tgtgcggtca gacaccagcc tcattatatc ttggagagaa 4740 tctcacaagc caaatggacc taaagaatca gtccgttatc agttggcaat ctcacacctg 4800 gccctaattc ctgaaactcc tctaagacaa agtgaatttc caaatggaag gctcactctc 4860 cttgttacta gactgtctgg tggaaatatt tatgtgttaa aggttcttgc ctgccactct 4920 gaggaaatgt ggtgtacaga gagtcatcct gtcactgtgg aaatgtttaa cacaccagag 4980 aaaccttatt ccttggttcc agagaacact agtttgcaat ttaattggaa ggctccattg 5040 aatgttaacc tcatcagatt ttgggttgag ctacagaagt ggaaatacaa tgagttttac 5100 catgttaaaa cttcatgcag ccaaggtcct gcttatgtct gtaatatcac aaatctacaa 5160 ccttatactt catataatgt cagagtagtg gtggtttata agacgggaga aaatagcacc 5220 tcacttccag aaagctttaa gacaaaagct ggatcccaa ataaaccagg cattcccaaa 5280 ttactagaag ggagtaaaaa ttcaatacag tgggagaaag ctgaagataa tggatgtaga 5340 attacatact atatccttga gataagaaag agcacttcaa ataatttaca gaaccagaat 5400 ttaaggtgga agatgacatt taatggatcc tgcagtagtg tttgcacatg gaagtccaaa 5460 aacctgaaag gaatatttca gttcagagta gtagctgcaa ataatctagg gtttggtgaa 5520 tatagtggaa tcagtgagaa tattatatta gttggagatg atttttggat accagaaaca 5580 agtttcatac ttactattat agttggaata tttctggttg ttacaatccc actgaccttt 5640 gtctggcata gaagattaaa gaatcaaaaa agtgccaagg aaggggtgac agtgcttata 5700 aacgaagaca aagagttggc tgagctgcga ggtctggcag ccggagtagg cctggctaat 5760 gcctgctatg caatacatac tcttccaacc caagaggaga ttgaaaatct tcctgccttc 5820 cctcgggaaa aactgactct gcgtctcttg ctgggaagtg gagcctttgg agaagtgtat 5880 gaaggaacag cagtggacat cttaggagtt ggaagtggag aaatcaaagt agcagtgaag 5940 actttgaaga agggttccac agaccaggag aagattgaat tcctgaagga ggcacatctg 6000 atgagcaaat ttaatcatcc caacattctg aagcagcttg gagtttgtct gctgaatgaa 6060 ccccaataca ttatcctgga actgatggag ggaggagacc ttcttactta tttgcgtaaa 6120 gcccggatgg caacgtttta tggtccttta ctcaccttgg ttgaccttgt agacctgtgt 6180 gtagatattt caaaaggctg tgtctacttg gaacggatgc atttcattca cagggatctg 6240 gcagctagaa attgccttgt ttccgtgaaa gactatacca gtccacggat agtgaagatt 6300 ggagactttg gactcgccag agacatctat aaaaatgatt actatagaaa gagaggggaa 6360 ggcctgctcc cagttcggtg gatggctcca gaaagtttga tggatggaat cttcactact 6420 caatctgatg tatggtcttt tggaattctg atttgggaga ttttaactct tggtcatcag 6480 ccttatccag ctcattccaa ccttgatgtg ttaaactatg tgcaaacagg agggagactg 6540 gagccaccaa gaaattgtcc tgatgatctg tggaatttaa tgacccagtg ctgggctcaa 6600 gaacccgacc aaagacctac ttttcataga attcaggacc aacttcagtt attcagaaat 6660 tttttcttaa atagcattta taagtccaga gatgaagcaa acaacagtgg agtcataaat 6720 gaaagctttg aaggtgaaga tggcgatgtg atttgtttga attcagatga cattatgcca 6780 gttgctttaa tggaaacgaa gaaccgagaa gggttaaact atatggtact tgctacagaa 6840 tgtggccaag gtgaagaaaa gtctgagggt cctctaggct cccaggaatc tgaatcttgt 6900 ggtctgagga aagaagagaa ggaaccacat gcagacaaag atttctgcca agaaaaacaa 6960 gtggcttact gcccttctgg caagcctgaa ggcctgaact atgcctgtct cactcacagt 7020 ggatatggag atgggtctga ttaa 7044 <210> 47 <211> 2364 <212> DNA <213> Artificial Sequence <220> <223> SMO <400> 47 atggccgctg cccgcccagc gcgggggccg gagctcccgc tcctggggct gctgctgctg 60 ctgctgctgg gggacccggg ccggggggcg gcctcgagcg ggaacgcgac cgggcctggg 120 cctcggagcg cgggcgggag cgcgaggagg agcgcggcgg tgactggccc tccgccgccg 180 ctgagccact gcggccgggc tgccccctgc gagccgctgc gctacaacgt gtgcctgggc 240 tcggtgctgc cctacggggc cacctccaca ctgctggccg gagactcgga ctcccaggag 300 gaagcgcacg gcaagctcgt gctctggtcg ggcctccgga atgccccccg ctgctgggca 360 gtgatccagc ccctgctgtg tgccgtatac atgcccaagt gtgagaatga ccgggtggag 420 ctgcccagcc gtaccctctg ccaggccacc cgaggcccct gtgccatcgt ggagagggag 480 cggggctggc ctgacttcct gcgctgcact cctgaccgct tccctgaagg ctgcacgaat 540 gaggtgcaga acatcaagtt caacagttca ggccagtgcg aagtgccctt ggttcggaca 600 gacaacccca agagctggta cgaggacgtg gagggctgcg gcatccagtg ccagaacccg 660 ctcttcacag aggctgagca ccaggacatg cacagctaca tcgcggcctt cggggccgtc 720 acgggcctct gcacgctctt caccctggcc acattcgtgg ctgactggcg gaactcgaat 780 cgctaccctg ctgttattct cttctacgtc aatgcgtgct tctttgtggg cagcattggc 840 tggctggccc agttcatgga tggtgcccgc cgagagatcg tctgccgtgc agatggcacc 900 atgaggcttg gggagcccac ctccaatgag actctgtcct gcgtcatcat ctttgtcatc 960 gtgtactacg ccctgatggc tggtgtggtt tggtttgtgg tcctcaccta tgcctggcac 1020 acttccttca aagccctggg caccacctac cagcctctct cgggcaagac ctcctacttc 1080 cacctgctca cctggtcact cccctttgtc ctcactgtgg caatccttgc tgtggcgcag 1140 gtggatgggg actctgtgag tgggatttgt tttgtgggct acaagaacta ccgataccgt 1200 gcgggcttcg tgctggcccc aatcggcctg gtgctcatcg tgggaggcta cttcctcatc 1260 cgaggagtca tgactctgtt ctccatcaag agcaaccacc ccgggctgct gagtgagaag 1320 gctgccagca agatcaacga gaccatgctg cgcctgggca tttttggctt cctggccttt 1380 ggctttgtgc tcattacctt cagctgccac ttctacgact tcttcaacca ggctgagtgg 1440 gagcgcagct tccgggacta tgtgctatgt caggccaatg tgaccatcgg gctgcccacc 1500 aagcagccca tccctgactg tgagatcaag aatcgcccga gccttctggt ggagaagatc 1560 aacctgtttg ccatgtttgg aactggcatc gccatgagca cctgggtctg gaccaaggcc 1620 acgctgctca tctggaggcg tacctggtgc aggttgactg ggcagagtga cgatgagcca 1680 aagcggatca agaagagcaa gatgattgcc aaggccttct ctaagcggca cgagctcctg 1740 cagaacccag gccaggagct gtccttcagc atgcacactg tgtccccacga cgggcccgtg 1800 gcgggcttgg cctttgacct caatgagccc tcagctgatg tctcctctgc ctgggcccag 1860 catgtcacca agatggtggc tcggagagga gccatactgc cccaggatat ttctgtcacc 1920 cctgtggcaa ctccagtgcc cccagaggaa caagccaacc tgtggctggt tgaggcagag 1980 atctccccag agctgcagaa gcgcctgggc cggaagaaga agaggaggaa gaggaagaag 2040 gaggtgtgcc cgctggcgcc gccccctgag cttcaccccc ctgcccctgc ccccagtacc 2100 attcctcgac tgcctcagct gccccggcag aaatgcctgg tggctgcagg tgcctgggga 2160 gctggggact cttgccgaca gggagcgtgg accctggtct ccaacccatt ctgcccagag 2220 cccagtcccc ctcaggatcc atttctgccc agtgcaccgg cccccgtggc atgggctcat 2280 ggccgccgac agggcctggg gcctattcac tcccgcacca acctgatgga cacagaactc 2340 atggatgcag actcggactt ctga 2364 <210> 48 <211> 1302 <212> DNA <213> Artificial Sequence <220> <223> STK11 <400> 48 atggaggtgg tggacccgca gcagctgggc atgttcacgg agggcgagct gatgtcggtg 60 ggtatggaca cgttcatcca ccgcatcgac tccaccgagg tcatctacca gccgcgccgc 120 aagcgggcca agctcatcgg caagtacctg atgggggacc tgctggggga aggctcttac 180 ggcaaggtga aggaggtgct ggactcggag acgctgtgca ggagggccgt caagatcctc 240 aagaagaaga agttgcgaag gatccccaac ggggaggcca acgtgaagaa ggaaattcaa 300 ctactgagga ggttacggca caaaaatgtc atccagctgg tggatgtgtt atacaacgaa 360 gagaagcaga aaatgtatat ggtgatggag tactgcgtgt gtggcatgca ggaaatgctg 420 gacagcgtgc cggagaagcg tttcccagtg tgccaggccc acgggtactt ctgtcagctg 480 attgacggcc tggagtacct gcatagccag ggcattgtgc acaaggacat caagccgggg 540 aacctgctgc tcaccaccgg tggcaccctc aaaatctccg acctgggcgt ggccgaggca 600 ctgcacccgt tcgcggcgga cgacacctgc cggaccagcc agggctcccc ggctttccag 660 ccgcccgaga ttgccaacgg cctggacacc ttctccggct tcaaggtgga catctggtcg 720 gctggggtca ccctctacaa catcaccacg ggtctgtacc ccttcgaagg ggacaacatc 780 tacaagttgt ttgagaacat cgggaagggg agctacgcca tcccgggcga ctgtggcccc 840 ccgctctctg acctgctgaa agggatgctt gagtacgaac cggccaagag gttctccatc 900 cggcagatcc ggcagcacag ctggttccgg aagaaacatc ctccggctga agcaccagtg 960 cccatcccac cgagcccaga caccaaggac cggtggcgca gcatgactgt ggtgccgtac 1020 ttggaggacc tgcacggcgc ggacgaggac gaggacctct tcgacatcga ggatgacatc 1080 atctacactc aggacttcac ggtgcccgga caggtcccag aagaggaggc cagtcacaat 1140 ggacagcgcc ggggcctccc caaggccgtg tgtatgaacg gcacagaggc ggcgcagctg 1200 agcaccaaat ccagggcgga gggccgggcc cccaaccctg cccgcaaggc ctgctccgcc 1260 agcagcaaga tccgccggct gtcggcctgc aagcagcagt ga 1302 <210> 49 <211> 1065 <212> DNA <213> Artificial Sequence <220> <223> TP53 <400> 49 atggatgatt tgatgctgtc cccggacgat attgaacaat ggttcactga agacccaggt 60 ccagatgaag ctcccagaat gccagaggct gctccccccg tggcccctgc accagcagct 120 cctacaccgg cggcccctgc accagccccc tcctggcccc tgtcatcttc tgtcccttcc 180 cagaaaacct accagggcag ctacggtttc cgtctgggct tcttgcattc tgggacagcc 240 aagtctgtga cttgcacgta ctcccctgcc ctcaacaaga tgttttgcca actggccaag 300 acctgccctg tgcagctgtg ggttgattcc acacccccgc ccggcacccg cgtccgcgcc 360 atggccatct acaagcagtc acagcacatg acggaggttg tgaggcgctg cccccaccat 420 gagcgctgct cagatagcga tggtctggcc cctcctcagc atcttatccg agtggaagga 480 aatttgcgtg tggagtattt ggatgacaga aacacttttc gacatagtgt ggtggtgccc 540 tatgagccgc ctgaggttgg ctctgactgt accaccatcc actacaacta catgtgtaac 600 agttcctgca tgggcggcat gaaccggagg cccatcctca ccatcatcac actggaagac 660 tccagtggta atctactggg acggaacagc tttgaggtgc gtgtttgtgc ctgtcctggg 720 agagaccggc gcacagagga agagaatctc cgcaagaaag gggagcctca ccacgagctg 780 cccccaggga gcactaagcg agcactgccc aacaacacca gctcctctcc ccagccaaag 840 aagaaaccac tggatggaga atatttcacc cttcagatcc gtgggcgtga gcgcttcgag 900 atgttccgag agctgaatga ggccttggaa ctcaaggatg cccaggctgg gaaggagcca 960 ggggggagca gggctcactc cagccacctg aagtccaaaa agggtcagtc tacctcccgc 1020 cataaaaaac tcatgttcaa gacagaaggg cctgactcag actga 1065 <210> 50 <211> 3495 <212> DNA <213> Artificial Sequence <220> <223> TSC1 <400> 50 atggcccaac aagcaaatgt cggggagctt cttgccatgc tggactcccc catgctgggt 60 gtgcgggacg acgtgacagc tgtctttaaa gagaacctca attctgaccg tggccctatg 120 cttgtaaaca ccttggtgga ttattacctg gaaaccagct ctcagccggc attgcacatc 180 ctgaccacct tgcaagagcc acatgacaag cacctcttgg acaggattaa cgaatatgtg 240 ggcaaagccg ccactcgttt atccatcctc tcgttactgg gtcatgtcat aagactgcag 300 ccatcttgga agcataagct ctctcaagca cctcttttgc cttctttact aaaatgtctc 360 aagatggaca ctgacgtcgt tgtcctcaca acaggcgtct tggtgttgat aaccatgcta 420 ccaatgattc cacagtctgg gaaacagcat cttcttgatt tctttgacat ttttggccgt 480 ctgtcatcat ggtgcctgaa gaaaccaggc cacgtggcgg aagtctatct cgtccatctc 540 catgccagtg tgtacgcact ctttcatcgc ctttatggaa tgtacccttg caacttcgtc 600 tcctttttgc gttctcatta cagtatgaaa gaaaacctgg agacttttga agaagtggtc 660 aagccaatga tggagcatgt gcgaattcat ccggaattag tgactggatc caaggaccat 720 gaactggacc ctcgaaggtg gaagagatta gaaactcatg atgttgtgat cgagtgtgcc 780 aaaatctctc tggatcccac agaagcctca tatgaagatg gctattctgt gtctcaccaa 840 atctcagccc gctttcctca tcgttcagcc gatgtcacca ccagccctta tgctgacaca 900 cagaatagct atgggtgtgc tacttctacc ccttactcca cgtctcggct gatgttgtta 960 aatatgccag ggcagctacc tcagactctg agttccccat cgacacggct gataactgaa 1020 ccaccacaag ctactctttg gagcccatct atggtttgtg gtatgaccac tcctccaact 1080 tctcctggaa atgtcccacc tgatctgtca cacccttaca gtaaagtctt tggtacaact 1140 gcaggtggaa aaggaactcc tctgggaacc ccagcaacct ctcctcctcc agccccactc 1200 tgtcattcgg atgactacgt gcacatttca ctcccccagg ccacagtcac accccccagg 1260 aaggaagaga gaatggattc tgcaagacca tgtctacaca gacaacacca tcttctgaat 1320 gacagaggat cagaagagcc acctggcagc aaaggttctg tcactctaag tgatcttcca 1380 gggtttttag gtgatctggc ctctgaagaa gatagtattg aaaaagataa agaagaagct 1440 gcaatatcta gagaactttc tgagatcacc acagcagagg cagagcctgt ggttcctcga 1500 ggaggctttg actctccctt ttaccgagac agtctcccag gttctcagcg gaagacccac 1560 tcggcagcct ccagttctca gggcgccagc gtgaaccctg agcctttaca ctcctccctg 1620 gacaagcttg ggcctgacac accaaagcaa gcctttactc ccatagacct gccctgcggc 1680 agtgctgatg aaagccctgc gggagacagg gaatgccaga cttctttgga gaccagtatc 1740 ttcactccca gtccttgtaa aattccacct ccgacgagag tgggctttgg aagcgggcag 1800 cctcccccgt atgatcatct ttttgaggtg gcattgccaa agacagccca tcattttgtc 1860 atcaggaaga ctgaggagct gttaaagaaa gcaaaaggaa acacagagga agatggtgtg 1920 ccctctacct ccccaatgga agtgctggac agactgatac agcagggagc agacgcgcac 1980 agcaaggagc tgaacaagtt gcctttaccc agcaagtctg tcgactggac ccactttgga 2040 ggctctcctc cttcagatga gatccgcacc ctccgagacc agttgctttt actgcacaac 2100 cagttactct atgagcgttt taagaggcag cagcatgccc tccggaacag gcggctcctc 2160 cgcaaggtga tcaaagcagc agctctggag gaacataatg ctgccatgaa agatcagttg 2220 aagttacaag agaaggacat ccagatgtgg aaggttagtc tgcagaaaga acaagctaga 2280 tacaatcagc tccaggagca gcgtgacact atggtaacca agctccacag ccagatcaga 2340 cagctgcagc atgaccgaga ggaattctac aaccagagcc aggaattaca gacgaagctg 2400 gaggactgca ggaacatgat tgcggagctg cggatagaac tgaagaaggc caacaacaag 2460 gtgtgtcaca ctgagctgct gctcagtcag gtttcccaaa agctctcaaa cagtgagtcg 2520 gtccagcagc agatggagtt cttgaacagg cagctgttgg ttcttgggga ggtcaacgag 2580 ctctatttgg aacaactgca gaacaagcac tcagatacca caaaggaagt agaaatgatg 2640 aaagccgcct atcggaaaga gctagaaaaa aacagaagcc atgttctcca gcagactcag 2700 aggcttgata cctcccaaaa acggattttg gaactggaat ctcacctggc caagaaagac 2760 caccttcttt tggaacagaa gaaatatcta gaggatgtca aactccaggc aagaggacag 2820 ctgcaggccg cagagagcag gtatgaggct cagaaaagga taacccaggt gtttgaattg 2880 gagatcttag atttatatgg caggttggag aaagatggcc tcctgaaaaa acttgaagaa 2940 gaaaaagcag aagcagctga agcagcagaa gaaaggcttg actgttgtaa tgacgggtgc 3000 tcagatcca tggtagggca caatgaagag gcatctggcc acaacggtga gaccaagacc 3060 cccaggccca gcagcgcccg gggcagtagt ggaagcagag gtggtggagg cagcagcagc 3120 agcagcagcg agctttctac cccagagaaa cccccacacc agagggcagg cccattcagc 3180 agtcggtggg agacgactat gggagaagcg tctgccagca tccccaccac tgtgggctca 3240 cttcccagtt caaaaagctt cctgggtatg aaggctcgag agttatttcg taataagagc 3300 gagagccagt gtgatgagga cggcatgacc agtagccttt ctgagagcct aaagacagaa 3360 ctgggcaaag acttgggtgt ggaagccaag attcccctga acctagatgg ccctcacccg 3420 tctcccccga ccccggacag tgttggacag ctacatatca tggactacaa tgagactcat 3480 catgaacaca gctaa 3495 <210> 51 <211> 5079 <212> DNA <213> Artificial Sequence <220> <223> TSC2 <400> 51 atggaatgtg gcctcaacaa tcgcatccgg atgatagggc agatttgtga agtcgcaaaa 60 accaagaaat ttgaagagca cgcagtggaa gcactctgga aggcggtcgc ggatctgttg 120 cagccggagc ggccgctgga ggcccggcac gcggtgctgg ctctgctgaa ggccatcgtg 180 caggggcagg gcgagcgttt gggggtcctc agagccctct tctttaaggt catcaaggat 240 tacccttcca acgaagacct tcacgaaagg ctggaggttt tcaaggccct cacagacaat 300 gggagacaca tcacctactt ggaggaagag ctggctgact ttgtcctgca gtggatggat 360 gttggcttgt cctcggaatt ccttctggtg ctggtgaact tggtcaaatt caatagctgt 420 tacctcgacg agtacatcgc aaggatggtt cagatgatct gtctgctgtg cgtccggacc 480 gcgtcctctg tggacataga ggtctccctg caggtgctgg acgccgtggt ctgctacaac 540 tgcctgccgg ctgagagcct cccgctgttc atcgttaccc tctgtcgcac catcaacgtc 600 aaggagctct gcgagccttg ctggaagctg atgcggaacc tccttggcac ccacctgggc 660 cacagcgcca tctacaacat gtgccacctc atggaggaca gagcctacat ggaggacgcg 720 cccctgctga gaggagccgt gttttttgtg ggcatggctc tctggggagc ccaccggctc 780 tattctctca ggaactcgcc gacatctgtg ttgccatcat tttaccaggc catggcatgt 840 ccgaacgagg tggtgtccta tgagatcgtc ctgtccatca ccaggctcat caagaagtat 900 aggaaggagc tccaggtggt ggcgtgggac attctgctga acatcatcga acggctcctt 960 cagcagctcc agaccttgga cagcccggag ctcaggacca tcgtccatga cctgttgacc 1020 acggtggagg agctgtgtga ccagaacgag ttccacgggt ctcaggagag atactttgaa 1080 ctggtggaga gatgtgcgga ccagaggcct gagtcctccc tcctgaacct gatctcctat 1140 agagcgcagt ccatccaccc ggccaaggac ggctggattc agaacctgca ggcgctgatg 1200 gagagattct tcaggagcga gtcccgaggc gccgtgcgca tcaaggtgct ggacgtgctg 1260 tcctttgtgc tgctcatcaa caggcagttc tatgaggagg agctgattaa ctcagtggtc 1320 atctcgcagc tctcccacat ccccgaggat aaagaccacc aggtccgaaa gctggccacc 1380 cagttgctgg tggacctggc agagggctgc cacacacacc acttcaacag cctgctggac 1440 atcatcgaga aggtgatggc ccgctccctc tccccacccc cggagctgga agaaagggat 1500 gtggccgcat actcggcctc cttggaggat gtgaagacag ccgtcctggg gcttctggtc 1560 atccttcaga ccaagctgta caccctgcct gcaagccacg ccacgcgtgt gtatgagatg 1620 ctggtcagcc acatcagct ccactacaag cacagctaca ccctgccaat cgcgagcagc 1680 atccggctgc aggcctttga cttcctgttg ctgctgcggg ccgactcact gcaccgcctg 1740 ggcctgccca acaaggatgg agtcgtgcgg ttcagcccct actgcgtctg cgactacatg 1800 gagccagaga gaggctctga gaagaagacc agcggccccc tttctcctcc cacagggcct 1860 cctggcccgg cgcctgcagg ccccgccgtg cggctggggt ccgtgcccta ctccctgctc 1920 ttccgcgtcc tgctgcagtg cttgaagcag gagtctgact ggaaggtgct gaagctggtt 1980 ctgggcaggc tgcctgagtc cctgcgctat aaagtgctca tctttacttc cccttgcagt 2040 gtggaccagc tgtgctctgc tctctgctcc atgctttcag gcccaaagac actggagcgg 2100 ctccgaggcg ccccagaagg cttctccaga actgacttgc acctggccgt ggttccagtg 2160 ctgacagcat taatctctta ccataactac ctggacaaaa ccaaacagcg cgagatggtc 2220 tactgcctgg agcagggcct catccaccgc tgtgccagcc agtgcgtcgt ggccttgtcc 2280 atctgcagcg tggagatgcc tgacatcatc atcaaggcgc tgcctgttct ggtggtgaag 2340 ctcacgcaca tctcagccac agccagcatg gccgtcccac tgctggagtt cctgtccact 2400 ctggccaggc tgccgcacct ctacaggaac tttgccgcgg agcagtatgc cagtgtgttc 2460 gccatctccc tgccgtacac caacccctcc aagtttaatc agtacatcgt gtgtctggcc 2520 catcacgtca tagccatgtg gttcatcagg tgccgcctgc ccttccggaa ggattttgtc 2580 cctttcatca ctaagggcct gcggtccaat gtcctcttgt cttttgatga cacccccgag 2640 aaggacagct tcagggcccg gagtactagt ctcaacgaga gacccaagag caggatacag 2700 acgtccctca ccagtgccag cttggggtct gcagatgaga actccgtggc ccaggctgac 2760 gatagcctga aaaacctcca cctggagctc acggaaacct gtctggacat gatggctcga 2820 tacgtcttct ccaacttcac ggctgtcccg aagaggtctc ctgtgggcga gttcctccta 2880 gcgggtggca ggaccaaaac ctggctggtt gggaacaagc ttgtcactgt gacgacaagc 2940 gtgggaaccg ggacccggtc gttactaggc ctggactcgg gggagctgca gtccggcccg 3000 gagtcgagct ccagccccgg ggtgcatgtg agacagacca aggaggcgcc ggccaagctg 3060 gagtcccagg ctgggcagca ggtgtcccgt ggggcccggg atcgggtccg ttccatgtcg 3120 gggggccatg gtcttcgagt tggcgccctg gacgtgccgg cctcccagtt cctgggcagt 3180 gccacttctc caggaccacg gactgcacca gccgcgaaac ctgagaaggc ctcagctggc 3240 acccgggttc ctgtgcagga gaagacgaac ctggcggcct atgtgcccct gctgacccag 3300 ggctgggcgg agatcctggt ccggaggccc acagggaaca ccagctggct gatgagcctg 3360 gagaacccgc tcagcccttt ctcctcggac atcaacaaca tgcccctgca ggagctgtct 3420 aacgccctca tggcggctga gcgcttcaag gagcaccggg acacagccct gtacaagtca 3480 ctgtcggtgc cggcagccag cacggccaaa ccccctcctc tgcctcgctc caacacagac 3540 tccgccgtgg tcatggagga gggaagtccg ggcgaggttc ctgtgctggt ggagccccca 3600 gggttggagg acgttgaggc agcgctaggc atggacaggc gcacggatgc ctacagcagg 3660 tcgtcctcag tctccagcca ggaggagaag tcgctccacg cggaggagct ggttggcagg 3720 ggcatcccca tcgagcgagt cgtctcctcg gagggtggcc ggccctctgt ggacctctcc 3780 ttccagccct cgcagcccct gagcaagtcc agctcctctc ccgagctgca gactctgcag 3840 gacatcctcg gggaccctgg ggacaaggcc gacgtgggcc ggctgagccc tgaggttaag 3900 gcccggtcac agtcagggac cctggacggg gaaagtgctg cctggtcggc ctcgggcgaa 3960 gacagtcggg gccagcccga gggtcccttg ccttccagct ccccccgctc gcccagtggc 4020 ctccggcccc gaggttacac catctccgac tcggccccat cacgcagggg caagagagta 4080 gagagggacg ccttaaagag cagagccaca gcctccaatg cagagaaagt gccaggcatc 4140 aaccccagtt tcgtgttcct gcagctctac cattccccct tctttggcga cgagtcaaac 4200 aagccaatcc tgctgcccaa tgagtcacag tcctttgagc ggtcggtgca gctcctcgac 4260 cagatcccat catacgacac ccacaagatc gccgtcctgt atgttggaga aggccagagc 4320 aacagcgagc tcgccatcct gtccaatgag catggctcct acaggtacac ggagttcctg 4380 acgggcctgg gccggctcat cgagctgaag gactgccagc cggacaaggt gtacctggga 4440 ggcctggacg tgtgtggtga ggacggccag ttcacctact gctggcacga tgacatcatg 4500 caagccgtct tccacatcgc caccctgatg cccaccaagg acgtggacaa gcaccgctgc 4560 gacaagaagc gccacctggg caacgacttt gtgtccattg tctacaatga ctccggtgag 4620 gacttcaagc ttggcaccat caagggccag ttcaactttg tccacgtgat cgtcaccccg 4680 ctggactacg agtgcaacct ggtgtccctg cagtgcagga aagacatgga gggccttgtg 4740 gacaccagcg tggccaagat cgtgtctgac cgcaacctgc ccttcgtggc ccgccagatg 4800 gccctgcacg caaatatggc ctcacaggtg catcatagcc gctccaaccc caccgatatc 4860 tacccctcca agtggattgc ccggctccgc cacatcaagc ggctccgcca gcggatctgc 4920 gaggaagccg cctactccaa ccccagccta cctctggtgc accctccgtc ccatagcaaa 4980 gcccctgcac agactccagc cgagcccaca cctggctatg aggtgggcca gcggaagcgc 5040 ctcatctcct cggtggagga cttcaccgag tttgtgtga 5079

Claims (16)

delete delete delete delete delete delete delete delete It relates to a method of providing information for personalized treatment,
detecting a genetic mutation in a gene panel for biopsy analysis for target treatment of cancer prepared in advance from a biological sample isolated from an individual;
setting a gene in which the detected genetic mutation has occurred as a therapeutic target for the individual; and
isolating a nucleic acid from a biological sample isolated from the subject;
The nucleic acid is cell-free DNA (cell-free DNA: cfDNA) and circulating tumor cell (CTC)-derived DNA is included together,
The information for the personalized treatment is cancer diagnosis, cancer prognosis prediction, cancer progress monitoring, or a combination thereof,
The detected genetic mutations were T7469C mutation in BRCA2 , C3508G mutation in ERBB2 , C3149T mutation in ERBB2 , A1451G mutation in ESR1 , G1162A mutation in FGFR4 , C3785T mutation in PTCH1 , C3524C mutation in PTCH1 , C1062G mutation in PTCH1 If it is selected from the group consisting of mutations, further comprising the step of determining the subject as having or having a high probability of having gynecological cancer,
The genetic panel,
ALK (ALK receptor tyrosine kinase), AR (androgen receptor), BAP1 (BRCA1 associated protein 1), BRCA1 (BRCA1 DNA repair associated), BRCA2 (BRCA2 DNA repair associated), BTK (Bruton tyrosine kinase), CDK4 (cyclin dependent kinase) 4), ERBB2 (erb-b2 receptor tyrosine kinase 2), ERBB3 (erb-b2 receptor tyrosine kinase 3), ESR1 (estrogen receptor 1), FGFR1 (fibroblast growth factor receptor 1), FGFR2 (fibroblast growth factor receptor 2), FGFR3 (fibroblast growth factor receptor 3), FGFR4 (fibroblast growth factor receptor 4), MYCN (MYCN proto-oncogene, bHLH transcription factor), NOTCH1 (notch receptor 1), PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), PTCH1 (patched 1), RET (ret proto-oncogene), STK11 (serine/threonine kinase 11), TP53 (tumor protein p53), TSC1 (TSC complex subunit 1), and TSC2 (TSC complex subunit 2) A method of providing information for personalized treatment comprising a. delete delete The method according to claim 9, wherein the step of detecting the genetic variation comprises adding the isolated nucleic acid to the gene panel to perform next-generation sequencing (NGS). The method of claim 12 , wherein the detecting of the genetic variation comprises detecting the genetic variation by comparing the nucleotide sequence information obtained as a result of performing next-generation sequencing with the nucleotide sequence information of the normal control. The method of claim 13 , wherein the normal control is a peripheral blood mononuclear cell (PBMC) of the subject. delete delete

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