CN112400807A - Species and quantity identification method for collecting plant pollen based on pollinating insect osmia - Google Patents
Species and quantity identification method for collecting plant pollen based on pollinating insect osmia Download PDFInfo
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- 241000560543 Osmia Species 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 57
- 241000894007 species Species 0.000 title claims abstract description 15
- 241000238631 Hexapoda Species 0.000 claims abstract description 95
- 230000010152 pollination Effects 0.000 claims abstract description 51
- 238000011835 investigation Methods 0.000 claims abstract description 31
- 241000196324 Embryophyta Species 0.000 claims description 78
- 238000005070 sampling Methods 0.000 claims description 17
- 238000005516 engineering process Methods 0.000 claims description 8
- 230000000877 morphologic effect Effects 0.000 claims description 8
- 241000257303 Hymenoptera Species 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 37
- 239000000463 material Substances 0.000 description 9
- 239000002689 soil Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 3
- 239000012804 pollen sample Substances 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000560540 Megachilidae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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- A—HUMAN NECESSITIES
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a species and quantity identification method for collecting plant pollen based on pollination insects, which is characterized by comprising the following steps: designing a pollination insect nest tube placing mode according to a plant diversity investigation target; placing osmia hive tubes and releasing pollinating insects; recovering pollinated insect nest pipes and collecting pollen ball samples; identifying the pollen type in the pollen mass and determining the amount thereof.
Description
Technical Field
The invention belongs to the field of ecological civilization construction and biodiversity assessment, and particularly relates to an identification method for assessing the variety, the number and the biodiversity of flowering plants by collecting plant pollen samples based on pollinating insect osmia.
Background
"biodiversity" is the sum of the ecocomplexes formed by various organisms (including animals, plants, microorganisms) and the environment and various ecological processes associated therewith, including three levels of ecosystem, species and genes. Biodiversity is the condition on which human beings live, is the basis of sustainable development of the economy and society, and is the guarantee of ecological safety and food safety. Therefore, protection of biodiversity is of great significance to our mankind. Important to protect biodiversity is the development of biodiversity research studies. Sample collection, species classification and identification in the target research area are fundamental links for conducting biodiversity investigation studies. How to objectively collect a regional sample and quickly and simply identify the type and number of plant species becomes a key technical problem.
Osmia is a general name of Osmia of the family melidae (apoideae) the family melissa americana (Megachilidae), and is named because it nests in gaps and holes of walls by using materials such as soil. Osmia occurs for 1 generation each year, and the time of activities outside the nest is mainly in spring and early summer. In spring, the osmia panzer breaks cocoons to emerge, and the osmia panzer is mated, pollen is collected, and the osmia panzer is nested to lay eggs. The osmia panzer can collect and eat pollen of various plants. Food collection and nesting occupy 85% -90% of the activity time of osmia, and female bees need to fly back and forth dozens of times to complete a cell. The pollen group prepared by the female osmia veneriformis for offspring mainly comprises pollen and a small amount of nectar. The distance between pollen and honey of osmia japonica is about 50-150 m. Biological characteristics of osmia panzer include, 1, collecting pollen nectar: the osmia panzer can collect pollen and nectar of various plants back and forth within a certain distance range; 2. nesting habit: the osmia panzer can build a nest chamber in a manually placed bee nest pipe (made of reeds or special materials); 3. storing the pollen dough: osmia panzer carries pollen nectar collected from various plants into a nest, makes a pollen dough, and stores in the nest room, and then spawns. The pollen ball becomes the food source of the offspring osmia. The method is characterized in that a pollen cluster is prepared according to various plant pollen nectar collected by osmia in an area range, and a plant species and quantity identification method based on pollen collected by pollinating insect osmia is established by taking a plant body, namely the pollen nectar, and the pollen cluster in a nest pipe as a main line.
Disclosure of Invention
The invention aims to disclose a species and quantity identification method for collecting plant pollen based on pollination insects
The purpose of the invention is realized by the following technical scheme:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen mass and determining the amount thereof.
Further, the pollinating insect is a bee.
Further, the pollinating insect is osmia, preferably osmia labialis.
Further, in the step (1), the placing mode is as follows: within the target investigation region, an n-point sampling method is employed, preferably n is 5.
Further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
and D, setting the pollination insect moving distance of the points of the nest pipes.
Further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: placing 100-200 nest tubes and 200-500 osmia panzer heads in each nest box; the nest box is placed at a height of 40-100cm, preferably 50cm, the height of the nest box is 0.6-2 m, a piece of moist soil is arranged in the range of about 1 m, which is convenient for the osmia collection to be used as a nesting material to make mud seal of the nest room.
Further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 10-20 days, and then the osmia panzer nest tubes are collected into a room for storage; the pollen clusters of each cell in the nest tube are labeled indoors and stored in a 0-5 ℃ refrigerated cabinet.
Further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
Further preferably, the pollinating insect has a male-female ratio of 1: 1.
The application of the variety and quantity identification method based on the collection of plant pollen by pollinating insects in detecting and evaluating the variety and quantity of plant pollen and biodiversity in a target investigation region.
Has the advantages that:
1) the method can effectively collect plant pollen in a specific time length and a certain area range;
2) the method uniformly collects plant pollen samples in a certain area, thereby avoiding artificial sampling deviation;
3) the method saves human resources and economic cost;
description of the drawings:
FIG. 1 shows the arrangement of osmia panzer honeycomb tubes in a plant diversity investigation region;
Detailed Description
The following experimental examples and examples are intended to further illustrate but not limit the invention.
Example 1:
a kind and quantity identification method for collecting plant pollen based on pollinating insects comprises the following steps:
1) parcel osmia nest pipe
2) The osmia hive is placed on the trunk
3) Osmia panzer cocoon put into nest box to prevent rain indoors
4) Nest building, pollen collecting and spawning of osmia panzer
5) Osmia hive tube pollen samples;
example 2:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
further, the pollinating insect is a bee.
Further, in the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: each nest box is provided with 100 nest tubes and 200 osmia panzer heads; the nest box is placed for 40-100cm, preferably 50cm in height, the nest box is 0.6-2 m, preferably about 1 m, a piece of moist soil is guaranteed, and therefore the osmia panzer can be conveniently collected to be used as a nesting material to manufacture a mud seal of a cell.
Further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 10 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
Example 3:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
the pollinating insect is osmia labialis;
further, in the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: 300 nest tubes and 300 osmia are placed in each nest box; the nest box is placed at the height of 50cm, and the range of about 1 m of the nest box ensures that moist soil exists, so that the osmia is conveniently collected to be used as a nesting material to manufacture mud seal of a nest room;
further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 15 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
Example 4:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
the pollinating insect is osmia;
further, in the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: each nest box is provided with 200 nest tubes and 500 osmia bees; the nest box is placed at the height of 50cm, and the range of about 1 m of the nest box ensures that moist soil exists, so that the osmia is conveniently collected to be used as a nesting material to manufacture mud seal of a nest room;
further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 20 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
Example 5:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
the pollinating insect is osmia labialis.
In the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: each nest box is provided with 180 nest tubes and 400 nosed osmia panzer; the nest box is placed at a height of 50cm, a piece of moist soil is guaranteed within a range of about 1 m of the nest box, and therefore the osmia panzer is convenient to collect and serve as a nesting material to manufacture a mud seal of a nest room.
Further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 18 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
Example 6:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
further, the pollinating insect is osmia labialis;
further, in the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: each nest box is provided with 170 nest tubes and 300 osmia bees; the nest box is placed at a height of 55cm, and a piece of moist soil is guaranteed in a range of about 1.2 m, so that the osmia panzer is conveniently collected to be used as a nesting material to manufacture a mud seal of a cell;
further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 10-20 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; making the pollen dough into a determination sample, and identifying the species of the plant pollen by using a DNA barcode and a DNA metabaracoding technology;
the male-female ratio of the pollinating insects is 1: 1; the method is used for checking and evaluating the variety and quantity of plant pollen in a target investigation region and biodiversity, and the results are shown in a table 1:
TABLE 1 analysis of wild flowering plants present in the habitat surrounding apple orchard based on species and quantity identification method of pollinating insects collecting plant pollen
Example 7:
a method for identifying the variety and the quantity of plant pollen collected based on pollinating insects comprises the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen cluster and determining the number thereof;
the pollinating insect is osmia;
further, in the step (1), the placing mode is as follows: in a target investigation region, a 5-point sampling method is adopted;
further, the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; a central circular area is determined by taking d as a radius,
1-5 of Site: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
d, setting the moving distance of the pollination insects at the setting points of the 5 nest pipes;
further, in the step (2), a osmia hive is respectively placed at the position of each of the 5 sample points: placing 100-200 nest tubes and 200-500 osmia panzer heads in each nest box; the nest box is placed at a height of 45cm, and a piece of moist soil is guaranteed in a range of about 0.9 m, so that the osmia panzer is conveniently collected to be used as a nesting material to manufacture a mud seal of a nest chamber;
further, in the step (3), the nest tubes and the osmia panzer in the plant diversity investigation region are placed for 10-20 days, and then the osmia panzer nest tubes are collected into a room for storage; marking the pollen clusters of each cell in the nest pipe indoors and storing the pollen clusters in a 0-5 ℃ refrigerated cabinet;
further, in the step (4), the refrigerated pollen dough is dried in the air; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; making the pollen dough into a determination sample, and identifying the species of the plant pollen by using a DNA barcode and a DNA metabaracoding technology;
the male-female ratio of the pollinating insects is 1: 1;
the method is used for detecting and evaluating the variety and the quantity of the plant pollen in a target investigation region and biodiversity.
Claims (10)
1. A kind and quantity identification method for collecting plant pollen based on pollinating insects is characterized by comprising the following steps:
(1) designing a pollination insect nest tube placing mode according to a plant diversity investigation target;
(2) placing pollination insect nest tubes and releasing pollination insects;
(3) recovering pollinated insect nest pipes and collecting pollen ball samples;
(4) identifying the pollen type in the pollen mass and determining the amount thereof.
2. The method for identifying the type and quantity of plant pollen collected based on pollinating insects of claim 1, wherein said pollinating insects are bees.
3. The method for identifying the kind and quantity of plant pollen collected based on pollinating insects of claim 1, wherein said pollinating insects are osmia bees, preferably osmia labialis bees.
4. The method of claim 1 for identifying the type and quantity of plant pollen collected based on pollinating insects, comprising: in the step (1), the placing mode is as follows: within the target investigation region, an n-point sampling method is employed, preferably n is 5.
5. The method of claim 4 for identifying the type and quantity of pollen collected from a plant by pollinating insects, comprising: the 5-point sampling method comprises the following steps: firstly, setting a first sample point at the middle position of the area as a central point Site 1; determining a Site1 circular area by taking d as a radius according to a central point Site1, respectively arranging four sample points, namely Site2, Site3, Site4 and Site5, in four directions by taking 2d as a distance, wherein the two adjacent directions are vertical to each other; determining a central circle area by taking d as a radius, wherein Site 1-5: representing the setting point of the pollination insect nest pipe;
d, setting the moving distance of the pollination insects at the setting point of each nest pipe;
and D, setting the pollination insect moving distance of the points of the nest pipes.
6. The method of claim 1 for identifying the type and quantity of plant pollen collected based on pollinating insects, comprising: in the step (2), a osmia hive is respectively placed at the positions of 5 sample points: placing 100-200 nest tubes and 200-500 osmia panzer heads in each nest box; the nest box is placed 40-100cm, preferably 50cm high.
7. The method of claim 1 for identifying the type and quantity of plant pollen collected based on pollinating insects, comprising: placing the nest tubes and osmia panzer in the plant diversity investigation region for 10-20 days, and then collecting the osmia panzer nest tubes into a room for storage; the pollen clusters of each cell in the nest tube are labeled indoors and stored in a 0-5 ℃ refrigerated cabinet.
8. The method of claim 1 for identifying the type and quantity of plant pollen collected based on pollinating insects, comprising: in the step (4), airing the refrigerated pollen dough; making a sample for experimental observation and determination; identifying the plant species category of pollen grains in each pollen cluster by combining an electronic scanning electron microscope and a morphological characteristic classification method, and quantifying the number of pollen of each plant; the pollen dough is made into a determination sample, and the plant pollen species category is identified by using a DNA barcode and a DNA metabaracoding technology.
9. The method of claim 1 for identifying the type and quantity of plant pollen collected based on pollinating insects, comprising: the male-female ratio of the pollinating insects is 1: 1.
10. The application of the variety and quantity identification method based on the collection of plant pollen by pollinating insects in detecting and evaluating the variety and quantity of plant pollen and biodiversity in a target investigation region.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113917082A (en) * | 2021-10-11 | 2022-01-11 | 中国林业科学研究院资源昆虫研究所 | Method for identifying influence of local commercial crop pollination on exotic commercial crops |
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| CN1301536A (en) * | 1999-12-29 | 2001-07-04 | 中国科学院动物研究所 | Bee pollination inducing agent |
| EP2175022A1 (en) * | 2003-02-21 | 2010-04-14 | Phylogica Limited | Methods of construction biodiverse gene fragment libraries |
| CN106818641A (en) * | 2017-02-21 | 2017-06-13 | 中国农业科学院蜜蜂研究所 | The method that Eastern bee wild stocks quantity is assessed using drone accumulation regions |
| CN108872096A (en) * | 2018-07-11 | 2018-11-23 | 中国农业科学院蜜蜂研究所 | A kind of multifarious measurement method of honeybee herborization pollen |
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| CN1301536A (en) * | 1999-12-29 | 2001-07-04 | 中国科学院动物研究所 | Bee pollination inducing agent |
| EP2175022A1 (en) * | 2003-02-21 | 2010-04-14 | Phylogica Limited | Methods of construction biodiverse gene fragment libraries |
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| CN108872096A (en) * | 2018-07-11 | 2018-11-23 | 中国农业科学院蜜蜂研究所 | A kind of multifarious measurement method of honeybee herborization pollen |
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| TOPITZHOFER ELLEN: "Assessment of Pollen Diversity Available to Honey Bees (Hymenoptera: Apidae) in Major Cropping Systems During Pollination in the Western United States", 《JOURNAL OF ECONOMIC ENTOMOLOGY》 * |
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| CN113917082A (en) * | 2021-10-11 | 2022-01-11 | 中国林业科学研究院资源昆虫研究所 | Method for identifying influence of local commercial crop pollination on exotic commercial crops |
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