CN112375903A - Method for enhancing leaching of arsenic pyrite microorganisms - Google Patents

Method for enhancing leaching of arsenic pyrite microorganisms Download PDF

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CN112375903A
CN112375903A CN202011263263.3A CN202011263263A CN112375903A CN 112375903 A CN112375903 A CN 112375903A CN 202011263263 A CN202011263263 A CN 202011263263A CN 112375903 A CN112375903 A CN 112375903A
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arsenopyrite
leaching
magnetite
enhancing
microbial
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CN112375903B (en
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邓莎
顾帼华
贺国帅
杨玮
龙涛
肖巍
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Xian University of Architecture and Technology
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    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
    • C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B30/00—Obtaining antimony, arsenic or bismuth
    • C22B30/04—Obtaining arsenic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P10/00—Technologies related to metal processing
    • Y02P10/20—Recycling

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  • Chemical & Material Sciences (AREA)
  • Manufacturing & Machinery (AREA)
  • Materials Engineering (AREA)
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  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Geochemistry & Mineralogy (AREA)
  • Geology (AREA)
  • Manufacture And Refinement Of Metals (AREA)

Abstract

The invention discloses a method for enhancing the leaching of arsenic pyrite microorganisms. By adding magnetite into the arsenic pyrite microbial leaching system, the mass ratio of the magnetite to the arsenic pyrite is not more than 2: 1. Under the synergistic effect of the microorganisms and the primary battery formed between the arsenopyrite and the magnetite, the oxidative decomposition of the arsenopyrite is enhanced, the leaching rate is obviously improved, the leaching period is shortened, and compared with the leaching result of the microorganisms without the magnetite, the leaching rate is improved by 19-63%. The invention provides technical guidance for the practice of microbial preoxidation production of refractory gold ores which take arsenopyrite as a main gold-carrying mineral.

Description

Method for enhancing leaching of arsenic pyrite microorganisms
Technical Field
The invention belongs to the technical field of sulfide ore microbial metallurgy, and particularly relates to a method for enhancing arsenic pyrite microbial leaching.
Background
Gold mine is an important non-renewable mineral resource, and because of long-term exploitation, the storage capacity of the easily-treated gold mine is gradually reduced, and how to effectively develop the difficultly-treated gold mine becomes a research hotspot of scholars. The refractory gold ore is also called refractory gold ore, and generally, if the leaching rate of gold after the gold ore is subjected to a conventional cyanidation process is lower than 70%, the refractory gold ore is called refractory gold ore. Gold in refractory gold ores is difficult to contact directly with cyanide, mainly because gold is often encapsulated in other sulfide ores, such as pyrite and arsenopyrite, in a sub-microscopic form. In order to expose the encapsulated gold, the ore must be pretreated prior to cyanidation for gold extraction. Compared with other pretreatment processes, the microbial oxidation pretreatment has the advantages of convenient operation, less equipment investment, low production cost, small harm to the surrounding environment and the like, thereby having wide industrial application prospect.
However, the microbial oxidation process has the problems of long oxidation period, low pulp concentration, strict requirements on raw materials and the like, and particularly when the arsenic pyrite is treated into gold-loaded mineral difficultly-treated gold ore, the gold recovery rate is very low, so that the resource is seriously wasted. The main reason is that a large amount of arsenic is generated in the arsenic pyrite microbial oxidation process, and the high-concentration arsenic has obvious inhibition on the growth of microbial communities, so that the catalytic oxidation effect on the arsenic pyrite is inhibited, and the recovery of gold in the subsequent cyaniding gold leaching process is further influenced. Therefore, the development of methods for reducing the toxicity of arsenic to microorganisms, culturing arsenic-resistant microorganisms and accelerating the oxidation speed of arsenopyrite is the key point for promoting the progress of the microorganism preoxidation technology of refractory gold ores.
Disclosure of Invention
In order to overcome the defects of the prior art and solve the problems of long oxidation period and low leaching rate of the microorganisms on the arsenopyrite, the invention aims to provide a method for strengthening the leaching of the arsenopyrite microorganisms, namely, a certain amount of magnetite is added into an arsenopyrite microorganism leaching system to form a primary battery with the arsenopyrite, so that the aims of accelerating the leaching speed of the arsenopyrite microorganisms and improving the leaching rate of the arsenopyrite are fulfilled, and technical guidance is provided for the practice of the microorganism preoxidation production of refractory gold ores taking the arsenopyrite as a main gold-carrying mineral.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for enhancing the leaching of arsenopyrite microorganisms comprises the following steps:
crushing an arsenopyrite ore sample and a magnetite ore sample into particles of 0.037-0.074 mm;
step (2), the prepared 9K culture medium is filled into a shake flask and sterilized at high temperature and high pressure;
step (3), adding an arsenopyrite ore sample into the shake flask filled with the 9K culture medium, adding the magnetite ore sample, and inoculating ore leaching microorganisms;
and (4) placing the shake flask obtained in the step (3) into a constant-temperature shaking table for cultivation.
The 9K culture medium is an iron-free 9K culture medium and comprises the following components: (NH)4)2SO4 3g/L,K2HPO4 0.5g/L,MgSO4·7H2O 0.5g/L,Ca(NO3)2 0.01g/L,KCl 0.1g/L。
After the arsenopyrite ore sample is added in the step (3), the concentration of the ore pulp of the arsenopyrite ore sample is not more than 5% by weight.
The mass ratio of the magnetite sample to the arsenopyrite sample is not more than 2: 1.
The mineral leaching microorganism is acidophilic archaea Ferroplasma thermophilum, Fe2+Or S0One or more of cultured Sulfobacillus thermophilus thermosulfoxides and Sulfobacillus acidobacter caldus.
The bacterial concentration of the bacterial liquid initially supplied for inoculation in the step (3) is 1.0 multiplied by 108one/ml-5.0X 108One/ml, the bacterial concentration in the leaching system after inoculation is 1.0 multiplied by 107one/ml-5.0X 107One/ml, the initial pH of the leaching system is the optimum pH for bacterial growth.
And (4) in the step (4), the temperature of the constant-temperature shaking table is 45-53 ℃, the rotating speed is 160-180 rpm, and the leaching time is 4-18 days.
According to the invention, a certain amount of magnetite is added into a microbial leaching system of the arsenic pyrite to form a primary battery with the arsenic pyrite, so that under the synergistic effect of the microbes and the primary battery, the oxidative decomposition of the arsenic pyrite is remarkably promoted, and the leaching rate of arsenic is improved. Compared with the method for leaching arsenic pyrite by microorganisms without adding magnetite, the leaching rate is improved by 19-63%. The method has strong pertinence, and solves the problems of long leaching period and low leaching efficiency of the arsenic pyrite.
Detailed Description
The following examples are provided to explain embodiments of the present invention in detail.
The invention relates to a method for strengthening arsenic pyrite microbial leaching, which comprises the following steps:
and (1) crushing the arsenopyrite ore sample and the magnetite ore sample into particles of 0.037-0.074 mm.
In the invention, the arsenopyrite ore sample comprises the following components:
Fe33.93％，As43.58％，S22.49％。
the magnetite sample comprises the following components:
fe69.67%, O25.82%, and the balance impurities.
And (2) filling the prepared 9K culture medium into a shake flask, and performing high-temperature high-pressure sterilization.
Specifically, the invention selects an iron-free 9K culture medium, which comprises the following components: (NH)4)2SO4 3g/L,K2HPO40.5g/L,MgSO4·7H2O 0.5g/L,Ca(NO3)2 0.01g/L,KCl 0.1g/L。
And (3) adding an arsenopyrite sample into the shake flask filled with the 9K culture medium, wherein the concentration of ore pulp of the arsenopyrite sample is not more than 5% by weight, adding the magnetite sample, and inoculating mineral leaching microorganisms, wherein the mass ratio of the magnetite sample to the arsenopyrite sample is not more than 2: 1.
In the invention, the mineral leaching microorganism is acidophilic archaea Ferroplasma thermophilum (preservation number: CBCBSUCSU208123, strain number: L1) and Fe2+Or S0One or more of cultured mineral-leaching microorganisms such as Sulfobacillus thermothiooxidans (preservation number: CBCBSUCSU208043, strain number: YN22) and Thiobacillus Acidithiobacillus caldus (preservation number: CBSUCSU208026, strain number: S1) are preserved in China center for type culture Collection and located in the Wuchang region of Wuhan city, Hubei provinceThe above strain has been published in patent CN 105734285A in Wuhan university school, No. 299, eight-road.
The bacterial concentration of the bacterial liquid for initial inoculation is 1.0 multiplied by 108one/ml-5.0X 108One/ml, the bacterial concentration in the leaching system after inoculation is 1.0 multiplied by 107one/ml-5.0X 107One/ml, the initial pH of the leaching system is the optimum pH for bacterial growth.
And (4) placing the shake flask obtained in the step (3) in a constant-temperature shaking table for culturing, wherein the temperature of the constant-temperature shaking table is 45-53 ℃, the rotating speed is 160-180 rpm, and the leaching time is 4-18 days.
Hereinafter, the leaching scheme and effect of the present invention will be described by taking the above three strains as examples.
Example 1
A system for leaching arsenopyrite from acidophilic archaea Ferroplasma thermophilum and adding magnetite: the arsenopyrite and magnetite ore are crushed into particles of 0.037-0.074 mm, the ore pulp concentration of the arsenopyrite in a leaching system is 1%, the ore pulp concentration of the magnetite is 1%, and the bacteria liquid concentration for initial inoculation is 5 multiplied by 108One/ml, the bacterial concentration in the leaching system after inoculation is 5 multiplied by 107Per milliliter; the initial pH of the leaching system was 1.0. The shaker speed was 165rpm and the temperature was 48 ℃. The leaching rate of arsenic pyrite leached for 18 days is only 15.59 percent in an arsenic pyrite bacteria leaching system without adding magnetite; the leaching rate of the arsenopyrite in 18 days by the bacteria leaching system with the mass ratio of the magnetite to the arsenopyrite of 1:1 reaches 35.52%, and is improved by 19.93%.
Example 2
Fe2+The cultured Sulfobacillus thermophilus thermosulfoxidooxidans leaches a system of arsenopyrite and magnetite: the arsenopyrite and magnetite ore are crushed into particles of 0.037-0.074 mm, the ore pulp concentration of the arsenopyrite in a leaching system is 1%, the ore pulp concentration of the magnetite is 1%, and the bacteria concentration of bacteria liquid initially supplied for inoculation is 2 multiplied by 108One/ml, the bacterial concentration in the leaching system after inoculation is 2 multiplied by 107Per milliliter; the initial pH of the leaching system was 1.6. The shaker speed was 165rpm and the temperature was 48 ℃. Without addition of additivesIn the arsenopyrite bacteria leaching system with magnetite, the leaching rate of arsenopyrite in 4 days is only 18.18%; the leaching rate of the arsenopyrite in 4 days by the bacteria-containing leaching system with the mass ratio of the magnetite to the arsenopyrite being 1:1 reaches 63.24%, and is improved by 45.06%.
Example 3
S0The cultured Sulfobacillus thermophilus thermosulfoxidooxidans leaches a system of arsenopyrite and magnetite: the arsenopyrite and magnetite ore are crushed into particles of 0.037-0.074 mm, the ore pulp concentration of the arsenopyrite in a leaching system is 1%, the ore pulp concentration of the magnetite is 1%, and the bacteria liquid concentration for initial inoculation is 5 multiplied by 108One/ml, the bacterial concentration in the leaching system after inoculation is 5 multiplied by 107Per milliliter; the initial pH of the leaching system was 1.6. The shaker speed was 165rpm and the temperature was 48 ℃. The leaching rate of arsenopyrite leached for 10 days is only 28.76 percent in an arsenopyrite bacteria leaching system without adding magnetite; the leaching rate of arsenopyrite in 10 days by the bacteria leaching system with the mass ratio of magnetite to arsenopyrite of 1:1 reaches 63.54%, and is improved by 34.78%.
Example 4
Fe2+A system for leaching arsenic pyrite and adding magnetite by using cultured Sulfobacillus thermophilus thermosulfoxides and Acidithiobacillus caldus mixed bacteria: the arsenopyrite and magnetite ore are crushed into particles of 0.037-0.074 mm, the ore pulp concentration of the arsenopyrite in a leaching system is 1%, the ore pulp concentration of the magnetite is 1%, and the bacteria concentration of bacteria liquid initially supplied for inoculation is 2 multiplied by 108One/ml, the bacterial concentration in the leaching system after inoculation is 2 multiplied by 107Per milliliter; the initial pH of the leaching system was 1.6. The shaker speed was 165rpm and the temperature was 48 ℃. The two bacteria are mixed according to the concentration ratio of 2: 1. The leaching rate of arsenic pyrite leached for 4 days is only 25.35 percent in an arsenic pyrite bacteria leaching system without adding magnetite; the leaching rate of the arsenopyrite in 4 days by the bacteria-containing leaching system with the mass ratio of the magnetite to the arsenopyrite being 1:1 reaches 88.49%, and is improved by 63.14%.
The principle of the invention is as follows:
many minerals in nature have semiconductor properties, and the electrostatic potential of various minerals is different in size. The minerals with different electrostatic potential sizes are contacted with each other to form a primary battery, the minerals with lower electrostatic potential are used as anodes to generate oxidation reaction, the dissolution is accelerated, and the minerals with higher electrostatic potential are used as cathodes to be protected or reduced. Some conductive materials with higher electrostatic potential are added in the leaching process of the arsenic pyrite to generate primary battery reaction with the arsenic pyrite, so that the dissolution of the arsenic pyrite is promoted. The magnetite has strong conductivity, high electrostatic potential, stable structure and strong magnetism, is easy to recycle, and can be used as an ideal cathode material for strengthening leaching of arsenic pyrite in a primary battery.
After the microorganism is added, under the synergistic action of the microorganism and the primary battery formed between the arsenopyrite and the magnetite, the oxidative decomposition of the arsenopyrite is further enhanced, the leaching rate is obviously improved, the leaching period is shortened, and compared with the leaching result of the microorganism without the magnetite, the leaching rate can be improved by 19-63%.

Claims (7)

1. The method for enhancing the microbial leaching of arsenopyrite is characterized by comprising the following steps:
crushing an arsenopyrite ore sample and a magnetite ore sample into particles of 0.037-0.074 mm;
step (2), the prepared 9K culture medium is filled into a shake flask and sterilized at high temperature and high pressure;
step (3), adding an arsenopyrite ore sample into the shake flask filled with the 9K culture medium, adding the magnetite ore sample, and inoculating ore leaching microorganisms;
and (4) placing the shake flask obtained in the step (3) into a constant-temperature shaking table for cultivation.
2. The method of enhancing arsenopyrite microbial leaching according to claim 1, wherein said 9K medium is an iron-free 9K medium consisting of: (NH)4)2SO4 3g/L,K2HPO4 0.5g/L,MgSO4·7H2O 0.5g/L,Ca(NO3)20.01g/L,KCl 0.1g/L。
3. The method for enhancing microbial leaching of arsenopyrite according to claim 1, wherein the ore pulp concentration of the arsenopyrite sample is no greater than 5% by weight after the arsenopyrite sample is added in step (3).
4. The method for enhancing microbial leaching of arsenopyrite according to claim 1 or 3, wherein the mass ratio of magnetite-like samples to arsenopyrite-like samples is not more than 2: 1.
5. The method for enhanced microbial leaching of arsenopyrite according to claim 1, wherein said leaching microorganisms are the acidophilic fungus Ferroplasma thermophilum, Fe2+Or S0One or more of cultured Sulfobacillus thermophilus thermosulfoxides and Sulfobacillus acidobacter caldus.
6. The method for enhancing the microbial leaching of arsenopyrite according to claim 1 or 5, wherein the bacterial concentration of the bacterial solution initially inoculated in step (3) is 1.0 x 108one/ml-5.0X 108One/ml, the bacterial concentration in the leaching system after inoculation is 1.0 multiplied by 107one/ml-5.0X 107One/ml, the initial pH of the leaching system is the optimum pH for bacterial growth.
7. The method for enhancing the microbial leaching of arsenopyrite according to claim 1, wherein in the step (4), the temperature of the constant-temperature shaking table is 45-53 ℃, the rotating speed is 160-180 rpm, and the leaching time is 4-18 days.
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CN113293299A (en) * 2021-05-07 2021-08-24 云南省生态环境科学研究院 Resource utilization method for arsenic-containing hazardous waste

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Publication number Priority date Publication date Assignee Title
CN113293299A (en) * 2021-05-07 2021-08-24 云南省生态环境科学研究院 Resource utilization method for arsenic-containing hazardous waste

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