CN112359080A - Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose content - Google Patents
Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose content Download PDFInfo
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Abstract
The invention discloses a preparation method of xylo-oligosaccharide with high xylobiose and xylotriose content, which relates to the technical field of xylo-oligosaccharide preparation, and specifically comprises S1 and raw material pretreatment; s2, preparing a polyxylose aqueous solution; s3, carrying out solid-liquid separation; s4, preparing a polyxylose liquid; s5, impurity removal, decoloration and interception and S6, and residual raw material treatment. According to the preparation method of xylo-oligosaccharide with high xylo-disaccharide and xylotriose content, edible acetic acid is added for fermentation through pretreatment of raw materials, so that xylan in the raw materials is more easily dissolved out when reacting with water and a weak acid catalyst, the dissolving-out amount of xylan is improved, the xylo-disaccharide and xylotriose content of xylo-oligosaccharide is improved, and the filtered raw material waste residue can be used for dissolving out residual xylan in the waste residue and forming feeding xylo-oligosaccharide through fermentation after the reaction of the weak acid catalyst and a feed fermenting agent, so that reasonable resource utilization is facilitated, the problem of subsequent treatment of the raw material waste residue is solved, and environmental pollution and resource waste are avoided.
Description
Technical Field
The invention relates to the technical field of xylo-oligosaccharide preparation, in particular to a preparation method of xylo-oligosaccharide with high xylobiose and xylotriose content.
Background
Xylo-oligosaccharide is also called xylo-oligosaccharide, is a functional polysaccharide formed by combining 2-7 xylose molecules by beta-1, 4 glycosidic bonds, can selectively promote the proliferation activity of intestinal bifidobacteria, the commonly used preparation method of xylo-oligosaccharide at present adopts a hydrothermal method mostly, and the xylo-oligosaccharide powder can be formed by mixing corn straws or wheat straws with water, adding a catalyst for cooking to dissolve out the xylo-oligosaccharide in the raw materials, adjusting the pH value of a xylan aqueous solution for enzymolysis reaction, then decoloring, filtering and compressing to prepare xylo-oligosaccharide syrup, and spray drying.
The existing hydrothermal method for preparing xylo-oligosaccharide has the advantages of no pollution, low production cost and the like, but the xylo-oligosaccharide prepared by the method has low xylobiose and xylotriose content which is about 40 percent of the total xylo-oligosaccharide due to the fact that the reaction between the raw materials and the catalyst is not violent.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of xylooligosaccharide with high xylobiose and xylotriose content, which solves the problems that the existing hydrothermal preparation method of xylooligosaccharide in the background art has the advantages of no pollution, low production cost and the like, but the prepared xylooligosaccharide has low xylobiose and xylotriose content which is about 40 percent of the total xylooligosaccharide due to the fact that the reaction between raw materials and a catalyst is not severe.
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents comprises the following operation steps:
s1, raw material pretreatment:
uniformly spraying edible acetic acid on the surface of the crushed raw material, and fermenting;
s2, preparing a polyxylose aqueous solution:
mixing the fermented raw material with clean water according to a weight ratio, adding a weak acid catalyst, and cooking until xylan in the raw material is dissolved out;
s3, solid-liquid separation:
filtering the prepared xylan aqueous solution, and separating residual raw material residues;
s4, preparing xylose-accumulating liquid:
adjusting the pH value of the polyxylose aqueous solution, and adding xylanase in proportion to carry out enzymolysis reaction;
s5, impurity removal, decoloration and interception:
removing impurities and decoloring the prepared xylooligosaccharide liquid through active carbon and exchange resin, intercepting macromolecular xylan through a macromolecular interception membrane, filtering out xylooligosaccharide liquid, continuously performing enzymolysis on the macromolecular xylan to generate xylooligosaccharide liquid, and performing vacuum concentration and spray drying on the xylooligosaccharide liquid to form xylooligosaccharide powder;
s6, residual raw material treatment:
collecting and compressing residual raw material residues, spraying a weak acid catalyst, adding a leavening agent for culture and fermentation, and scattering the mixture after the fermentation is finished to be uniformly mixed with animal feed.
Optionally, in step S1, the weight ratio of the raw material to the edible acetic acid is 1:0.2 to 1: 0.4.
Optionally, in step S1, the fermentation time is 0.2h to 0.35 h.
Optionally, in step S2, the raw material and the clean water are mixed according to a weight ratio of 1:6 to 1:10, and a weak acid catalyst is added, wherein the weak acid catalyst is added, and the weight percentage of the raw material is 0.2% to 1.5%.
Optionally, in step S2, the cooking temperature is 155 ℃ to 180 ℃, and the cooking time is 0.3h to 1 h. .
Optionally, in step S4, the PH of the polyxylose aqueous solution is adjusted to 5.0 to 6.0, and 50 to 85UI units of xylanase are added per gram of the polyxylose aqueous solution.
Optionally, the temperature during the enzymolysis reaction is 45 ℃ to 60 ℃, and the time is controlled to be 4h to 10 h.
Optionally, in step S6, the weight ratio of the residual raw material slag to the weak acid catalyst is 1: 0.15.
Optionally, in the step S6, the fermentation agent is a feed fermentation agent, and the addition amount of the feed fermentation agent is 0.2% of the weight percentage of the residual raw material residue.
The invention provides a preparation method of xylo-oligosaccharide with high xylobiose and xylotriose content, which has the following beneficial effects:
according to the preparation method of xylo-oligosaccharide with high xylo-disaccharide and xylotriose content, edible acetic acid is added for fermentation through pretreatment of raw materials, so that xylan in the raw materials is more easily dissolved out when reacting with water and a weak acid catalyst, the dissolving-out amount of xylan is improved, the xylo-disaccharide and xylotriose content of xylo-oligosaccharide is improved, and the filtered raw material waste residue can be used for dissolving out residual xylan in the waste residue and forming feeding xylo-oligosaccharide through fermentation after the reaction of the weak acid catalyst and a feed fermenting agent, so that reasonable resource utilization is facilitated, the problem of subsequent treatment of the raw material waste residue is solved, and environmental pollution and resource waste are avoided.
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FIG. 1 is a comparison diagram of the content detection results of xylobiose and xylotriose.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1, the present invention provides a technical solution: a method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents comprises the following operation steps:
s1, raw material pretreatment:
uniformly spraying edible acetic acid on the surface of the crushed raw material, and fermenting;
s2, preparing a polyxylose aqueous solution:
mixing the fermented raw material with clean water according to a weight ratio, adding a weak acid catalyst, and cooking until xylan in the raw material is dissolved out;
s3, solid-liquid separation:
filtering the prepared xylan aqueous solution, and separating residual raw material residues;
s4, preparing xylose-accumulating liquid:
adjusting the pH value of the polyxylose aqueous solution, and adding xylanase in proportion to carry out enzymolysis reaction;
s5, impurity removal, decoloration and interception:
removing impurities and decoloring the prepared xylooligosaccharide liquid through active carbon and exchange resin, intercepting macromolecular xylan through a macromolecular interception membrane, filtering out xylooligosaccharide liquid, continuously performing enzymolysis on the macromolecular xylan to generate xylooligosaccharide liquid, and performing vacuum concentration and spray drying on the xylooligosaccharide liquid to form xylooligosaccharide powder;
s6, residual raw material treatment:
collecting and compressing residual raw material residues, spraying a weak acid catalyst, adding a leavening agent for culture and fermentation, and scattering the mixture after the fermentation is finished to be uniformly mixed with animal feed.
In step S1, the weight ratio of the raw material to the edible acetic acid is 1:0.2 to 1: 0.4.
In step S1, the fermentation time is 0.2h to 0.35 h.
In step S2, the raw material and the clean water are mixed according to the weight ratio of 1:6 to 1:10, and a weak acid catalyst with the raw material weight percentage of 0.2% to 1.5% is added.
In step S2, the cooking temperature is 155 ℃ to 180 ℃, and the cooking time is 0.3h to 1 h. .
In step S4, the pH value of the polyxylose aqueous solution is adjusted to 5.0-6.0, and 50-85 UI units of xylanase is added into each gram of polyxylose aqueous solution.
The temperature of the enzymolysis reaction is 45 ℃ to 60 ℃, and the time is controlled to be 4h to 10 h.
In step S6, the weight ratio of the residual raw material slag to the weak acid catalyst is 1: 0.15.
In the step S6, the starter culture is a feed starter culture, and the addition amount of the feed starter culture is 0.2 percent of the weight percentage of the residual raw material slag;
detecting the content of xylobiose and xylotriose in the xylo-oligosaccharide prepared by different preparation methods,
the same amount of xylo-oligosaccharides prepared in different preparation methods are used for detection, and the difference lies in whether the raw materials are pretreated, namely whether edible acetic acid is sprayed on the surface of the raw materials, and the result is shown in fig. 1:
thereby obtaining xylo-oligosaccharide prepared by pretreatment, wherein the content of xylobiose and xylotriose of the xylo-oligosaccharide is obviously greater than that of xylobiose and xylotriose of the xylo-oligosaccharide prepared without pretreatment;
according to the preparation method of xylo-oligosaccharide with high xylo-disaccharide and xylotriose content, edible acetic acid is added for fermentation through pretreatment of raw materials, so that xylan in the raw materials is more easily dissolved out when reacting with water and a weak acid catalyst, the dissolving-out amount of xylan is improved, the xylo-disaccharide and xylotriose content of xylo-oligosaccharide is improved, and the filtered raw material waste residue can be used for dissolving out residual xylan in the waste residue and forming feeding xylo-oligosaccharide through fermentation after the reaction of the weak acid catalyst and a feed fermenting agent, so that reasonable resource utilization is facilitated, the problem of subsequent treatment of the raw material waste residue is solved, and environmental pollution and resource waste are avoided.
In conclusion, when the method for preparing xylo-oligosaccharide with high xylobiose and xylotriose content is used, the following operation steps are firstly carried out:
s1, raw material pretreatment: uniformly spraying edible acetic acid on the surface of the crushed raw material, and fermenting;
s2, preparing a polyxylose aqueous solution: mixing the fermented raw material with clean water according to a weight ratio, adding a weak acid catalyst, and cooking until xylan in the raw material is dissolved out;
s3, solid-liquid separation: filtering the prepared xylan aqueous solution, and separating residual raw material residues;
s4, preparing xylose-accumulating liquid: adjusting the pH value of the polyxylose aqueous solution, and adding xylanase in proportion to carry out enzymolysis reaction;
s5, impurity removal, decoloration and interception: removing impurities and decoloring the prepared xylooligosaccharide liquid through active carbon and exchange resin, intercepting macromolecular xylan through a macromolecular interception membrane, filtering out xylooligosaccharide liquid, continuously performing enzymolysis on the macromolecular xylan to generate xylooligosaccharide liquid, and performing vacuum concentration and spray drying on the xylooligosaccharide liquid to form xylooligosaccharide powder;
s6, residual raw material treatment: collecting and compressing residual raw material residues, spraying a weak acid catalyst, adding a leavening agent for culture and fermentation, and scattering the mixture after the fermentation is finished to be uniformly mixed with animal feed;
according to the preparation method of xylo-oligosaccharide with high xylo-disaccharide and xylotriose content, edible acetic acid is added for fermentation through pretreatment of raw materials, so that xylan in the raw materials is more easily dissolved out when reacting with water and a weak acid catalyst, the dissolving-out amount of xylan is improved, the xylo-disaccharide and xylotriose content of xylo-oligosaccharide is improved, and the filtered raw material waste residue can be used for dissolving out residual xylan in the waste residue and forming feeding xylo-oligosaccharide through fermentation after the reaction of the weak acid catalyst and a feed fermenting agent, so that reasonable resource utilization is facilitated, the problem of subsequent treatment of the raw material waste residue is solved, and environmental pollution and resource waste are avoided.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (9)
1. A method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents is characterized by comprising the following steps: the method comprises the following operation steps:
s1, raw material pretreatment:
uniformly spraying edible acetic acid on the surface of the crushed raw material, and fermenting;
s2, preparing a polyxylose aqueous solution:
mixing the fermented raw material with clean water according to a weight ratio, adding a weak acid catalyst, and cooking until xylan in the raw material is dissolved out;
s3, solid-liquid separation:
filtering the prepared xylan aqueous solution, and separating residual raw material residues;
s4, preparing xylose-accumulating liquid:
adjusting the pH value of the polyxylose aqueous solution, and adding xylanase in proportion to carry out enzymolysis reaction;
s5, impurity removal, decoloration and interception:
removing impurities and decoloring the prepared xylooligosaccharide liquid through active carbon and exchange resin, intercepting macromolecular xylan through a macromolecular interception membrane, filtering out xylooligosaccharide liquid, continuously performing enzymolysis on the macromolecular xylan to generate xylooligosaccharide liquid, and performing vacuum concentration and spray drying on the xylooligosaccharide liquid to form xylooligosaccharide powder;
s6, residual raw material treatment:
collecting and compressing residual raw material residues, spraying a weak acid catalyst, adding a leavening agent for culture and fermentation, and scattering the mixture after the fermentation is finished to be uniformly mixed with animal feed.
2. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S1, the weight ratio of the raw material to the edible acetic acid is 1:0.2 to 1: 0.4.
3. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S1, the fermentation time is 0.2h to 0.35 h.
4. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S2, the raw material and the clean water are mixed according to the weight ratio of 1:6 to 1:10, and a weak acid catalyst with the raw material weight percentage of 0.2% to 1.5% is added.
5. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S2, the cooking temperature is 155 ℃ to 180 ℃, and the cooking time is 0.3h to 1 h. .
6. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S4, the pH value of the polyxylose aqueous solution is adjusted to be 5.0-6.0, and 50-85 UI units of xylanase is added into each gram of the polyxylose aqueous solution.
7. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 6, wherein the steps of: the temperature of the enzymolysis reaction is 45 ℃ to 60 ℃, and the time is controlled to be 4h to 10 h.
8. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S6, the weight ratio of the residual raw material slag to the weak acid catalyst is 1: 0.15.
9. The method for preparing xylo-oligosaccharide with high xylobiose and xylotriose contents according to claim 1, wherein the method comprises the following steps: in the step S6, the starter culture is a feed starter culture, and the addition amount of the feed starter culture is 0.2% of the weight percentage of the residual raw material residues.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403591A (en) * | 2001-09-05 | 2003-03-19 | 中国农业大学 | Prepn of oligoxylose |
| CN102080116A (en) * | 2010-12-14 | 2011-06-01 | 南京林业大学 | Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis |
| CN104164520A (en) * | 2014-07-15 | 2014-11-26 | 南京林业大学 | Method using acetic acid for controlled catalysis of orientational hydrolysis of xylan to prepare multicomponent xylooligosaccharide |
| WO2017110975A1 (en) * | 2015-12-25 | 2017-06-29 | 東レ株式会社 | Method for producing xylooligosaccharide composition |
| CN108070621A (en) * | 2018-01-16 | 2018-05-25 | 南阳师范学院 | A kind of method of comprehensive utilization of maize straw |
| CN108707632A (en) * | 2018-05-25 | 2018-10-26 | 浙江畯和生物科技有限公司 | A kind of preparation method of xylo-oligosaccharide |
-
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- 2020-11-03 CN CN202011211798.6A patent/CN112359080A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403591A (en) * | 2001-09-05 | 2003-03-19 | 中国农业大学 | Prepn of oligoxylose |
| CN102080116A (en) * | 2010-12-14 | 2011-06-01 | 南京林业大学 | Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis |
| CN104164520A (en) * | 2014-07-15 | 2014-11-26 | 南京林业大学 | Method using acetic acid for controlled catalysis of orientational hydrolysis of xylan to prepare multicomponent xylooligosaccharide |
| WO2017110975A1 (en) * | 2015-12-25 | 2017-06-29 | 東レ株式会社 | Method for producing xylooligosaccharide composition |
| CN108070621A (en) * | 2018-01-16 | 2018-05-25 | 南阳师范学院 | A kind of method of comprehensive utilization of maize straw |
| CN108707632A (en) * | 2018-05-25 | 2018-10-26 | 浙江畯和生物科技有限公司 | A kind of preparation method of xylo-oligosaccharide |
Non-Patent Citations (5)
| Title |
|---|
| XIXUN HAO等: "Production of xylooligosaccharides and monosaccharides from hydrogen peroxide-acetic acid-pretreated poplar by two-step enzymatic hydrolysis", 《BIORESOURCE TECHNOLOGY》 * |
| 勇强等: "低聚木糖的生产与应用", 《新饲料》 * |
| 汪勇主编: "《粮油副产物加工学》", 31 January 2019 * |
| 王杏文等: "稀酸预处理促进酶对低聚木糖生产废渣的降解作用", 《第八届中国酶工程学术研讨会论文集》 * |
| 鞠美庭等编: "《生物质固废资源化技术手册》", 31 March 2014 * |
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Application publication date: 20210212 |