CN112353730A - Fenugreek seed extract body lotion and preparation method thereof - Google Patents

Fenugreek seed extract body lotion and preparation method thereof Download PDF

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CN112353730A
CN112353730A CN202011407930.0A CN202011407930A CN112353730A CN 112353730 A CN112353730 A CN 112353730A CN 202011407930 A CN202011407930 A CN 202011407930A CN 112353730 A CN112353730 A CN 112353730A
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fenugreek
parts
quercetin
zein
lecithin
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邓春林
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Abstract

The invention discloses a fenugreek seed extract body lotion and a preparation method thereof. The palmitic acid triglyceride can adsorb horny layer, and the residual alkalescence neutralizes skin pH value to prevent allergy. The fenugreek sterol ester improves cell activity, forms a protective film to lock water, and enhances moisturizing effect. Quercetin can scavenge free radicals in vivo, reduce iron overload in organism, and zein-quercetin-lecithin compound can improve lipid metabolism and provide nutrients for skin. The invention achieves the effects of preventing allergy, enhancing moisture retention, improving lipid metabolism and the like by adding the repairing substance and the reaction of the repairing substance in the organism.

Description

Fenugreek seed extract body lotion and preparation method thereof
Technical Field
The invention relates to the technical field of cosmetics, in particular to a body lotion extracted from fenugreek seeds and a preparation method thereof
The preparation method is as follows.
Background
The natural moisturizing system in human skin is mainly composed of water, lipids, and natural moisturizing factors. Lipids fill the stratum corneum cells in layers, and mainly function to form a water barrier to prevent water loss. The skin contains a structure that retains and releases water, and within the stratum corneum, the water content gradually decreases from the bottom layer to the upper layer. The bottom most layer of the stratum corneum acts as a water barrier, water permeating from the inside to the outside and to the surrounding atmosphere. In dry weather, the body is very easy to have dry skin, and the most remarkable main manifestation is that a lot of white dandruff appears on the skin, so that the skin is dry. Because of dehydration of skin, the water circulation of skin is not smooth, the metabolism of skin is slowed down, blood vessels supplying nutrition to skin are atrophied, the vitality of epidermal cells is weakened, subcutaneous fat is reduced, collagen, elastin and the like are lost, and finally, the formation of false wrinkles of skin can be caused. If water is not supplemented in time, the water circulation of the skin is unblocked, the skin can be loosened and lose elasticity after a long time, and the skin can be cracked continuously and then becomes painful or itchy, which is caused by dry skin. Water shortage is an indication, and the vicious circle of water is the source. Skin damage caused by water shortage troubles women, skin is unbalanced in water and oil, pores are large, comedones are bred, oil is seriously discharged, skin is aged due to dry skin, the skin cannot be automatically repaired, dark yellow and opaque skin color is caused, and dark pigments are accumulated on the face. The protective action of the top sebum membrane on the skin is also weakened, which causes the skin to become dry and itchy, and the resistance to external irritation is weakened, which makes the skin easily allergic. Skin problems have been increasing over the long term. The skin with sufficient moisture can resist the damage of external factors to the skin in a good state, and properly moisturizes water to keep the skin healthy.
In daily life, people usually only pay attention to the ultraviolet protection, fear that the skin is sensitive to acne growth, but neglect comprehensive water replenishing and repairing of the skin, so that the design of the fenugreek seed extract body lotion capable of repairing the skin allergy while replenishing water is necessary.
Disclosure of Invention
The invention aims to provide fenugreek seed extract body lotion to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme that the fenugreek seed extract body lotion comprises the following raw materials in parts by weight:
50-60 parts of fenugreek seeds, 30-50 parts of triglyceride palmitate, 40-60 parts of fenugreek palm oil, 40-60 parts of glycerol, 10-15 parts of fenugreek palmitic acid, 10-20 parts of fenugreek sterol, 10-20 parts of fatty acid, 5-10 parts of fenugreek sterol ester, 5-10 parts of fatty oil, 10-15 parts of zein, 10-15 parts of lecithin, 10-15 parts of quercetin, 5-10 parts of zein-quercetin-lecithin compound, 10-15 parts of sorbitol, 10-20 parts of absolute ethyl alcohol, 2-5 parts of preservative, 3-5 parts of essence and deionized water.
Preferably, the fenugreek palmitic acid is fenugreek fatty acid, after saponification, the soap solution is acidified by dilute sulfuric acid, the mixture is kept stand to obtain clear liquid, ether is used as an extracting agent to extract free fatty acid of the fenugreek, the fraction is obtained through reduced pressure distillation, and the fraction is cooled to obtain the fenugreek palmitic acid.
Preferably, the palmitic acid triglyceride and the derivatives thereof are prepared by carrying out catalytic reaction on fenugreek palmitic acid and glycerol, dissolving the reaction product in n-hexane, and then using superbase as a catalyst.
Preferably, the fenugreek sterol ester is prepared by esterification reaction of fenugreek sterol and fatty acid.
Preferably, the zein-quercetin-lecithin compound is prepared by mixing zein, quercetin and lecithin under magnetic stirring with heated ethanol.
Preferably, the preservative is one of formaldehyde-releasing preservatives such as sodium hydroxymethylglycinate, organic acid preservatives such as potassium sorbate, p-hydroxy and benzoate preservatives such as methylparaben, and the like.
In the technical scheme, the fenugreek palmitic acid is used as a softening agent and an emulsifying agent, and the saturated fatty acid is a main component in the fatty oil, can be used as a cleaning agent or a foaming agent and has the effects of moistening and cleaning the skin.
In the technical scheme, the palmitic acid triglyceride solid small particles can adsorb dark substances such as dead skin, dust and the like on the skin through rubbing and massage, and play a role in removing cutin. The trace residual alkalinity neutralizes the acid-base degree of skin, improves the instability of skin state, improves the photo-thermal stability, and has the effect of preventing allergy.
In the technical scheme, the free sterol is easier to be absorbed by the organism, and the cell membrane growth is promoted from the inside of the organism. The stigmasterol ester which can not be absorbed by organism remains in vitro, and a layer of protective film is formed on the surface of skin to lock the moisture of the skin, so that the moisturizing effect is enhanced.
In the technical scheme, the quercetin can eliminate free radicals in vivo, inhibit lipid peroxidation, reduce iron overload in organisms and reduce oxidative damage caused by iron overload. The zein-quercetin-lecithin compound enhances the thermal stability of the structure, improves lipid metabolism, and provides sufficient nutrients such as water and oxygen for the skin.
The invention provides a preparation method of fenugreek seed extract body milk, which comprises the following steps:
(1) extracting raw materials from fenugreek seeds:
extracting fatty acid of fenugreek, sterol of fenugreek, lecithin and quercetin;
(2) preparing fenugreek palmitic acid;
(3) preparing fenugreek palmitic acid triglyceride;
(4) preparing fenugreek sterol ester;
(5) preparing a zein-quercetin-lecithin compound;
(6) mixing sorbitol and glycerol, stirring, and adding into the substance obtained in step (2);
(7) adding the substance obtained in the step (3) into the step (5), and uniformly stirring;
(8) adding the substance obtained in the step (4) into the step (6), and uniformly stirring;
(9) adding the substance obtained in the step (7) into the step (8), and uniformly stirring;
(10) and (4) adding absolute ethyl alcohol, essence and preservative into the step (9), and mixing and stirring uniformly to obtain a finished product.
Preferably, the preparation method of the fenugreek palmitic acid in the step (2) comprises the following steps: adding a certain amount of fenugreek fatty acid, lacquer wax, a sodium hydroxide aqueous solution and a plurality of zeolites into a three-neck flask provided with a stirrer and a thermometer, electrically heating and boiling, refluxing and saponifying at 100-150 ℃, stopping heating after reacting for 3-5 h, acidifying the soap solution with dilute sulfuric acid after the saponified solution is cooled to 20-40 ℃ to enable the pH value of the soap solution to be about 4.0, then moving the soap solution into a separating funnel, standing and layering, separating out a clear solution layer, washing the residual solution with saturated salt water to enable the pH value to be neutral, extracting free fatty acid in the solution with diethyl ether as an extracting agent, distilling the extract at normal pressure, recovering the diethyl ether as a solvent for recycling, distilling in a reduced pressure distillation device, collecting fractions, and cooling to obtain the fenugreek palmitic acid.
Preferably, the preparation method of the fenugreek palmitic acid triglyceride in the step (3) comprises the following steps: glycerol and fenugreek palmitic acid are placed in a four-neck flask on a constant-temperature heating sleeve according to the mass ratio of 1:5, super-strong base is used as a catalyst, the reaction is carried out for 5-7 hours at the temperature of 150-250 ℃, nitrogen is continuously introduced as protective gas during the reaction, the temperature is reduced to room temperature after the reaction is finished, solid is separated out, n-hexane is added for dissolution, the super-strong base is removed through filtration, and the finished product is obtained through reduced pressure concentration.
Preferably, the preparation method of fenugreek sterol ester in the step (4) comprises the following steps: under the vacuum condition, excessive molten fatty acid is used as a reaction solvent, sodium bisulfate is used as a catalyst, the mass ratio of the fatty acid to the fenugreek sterol is 1.2:1, the reaction temperature is 100-200 ℃, and esterification reaction is directly carried out to generate fenugreek sterol ester.
Preferably, the method for preparing the zein-quercetin-lecithin compound in the step (5) comprises the following steps: dissolving 1.0g of zein in 100ml of 70% absolute ethyl alcohol, magnetically stirring for 1-2 h, adding lecithin to enable the mass ratio of the zein to the lecithin to be 2:3, magnetically stirring for 2-4 h, then adding 0.1g of quercetin into the mixed solution, magnetically stirring for 1-3 h, adding 20ml of zein-lecithin-quercetin ethanol solution into 60ml of deionized water, magnetically stirring for 20-40 min, evaporating ethanol remained in a dispersion body at 45 ℃ through a rotary evaporator, adjusting the dispersion body to PH40 by using a PH regulator, centrifuging for 20-30 min at 1500-2500 r/min to remove large particles and free quercetin, finally further analyzing the dispersion body, and freeze-drying for 40-60 h to obtain a finished product.
In the step (2), the fenugreek palmitic acid is used as a softening agent and an emulsifying agent, and the saturated fatty acid is a main component in the fatty oil and has the effects of moistening and cleaning the skin.
In the step (3), the palmitic acid triglyceride solid small particles can adsorb dark substances such as dead skin, dust and the like on the skin through rubbing and massage, and have the function of removing cutin. The trace residual alkalinity neutralizes the acid-base degree of skin, improves skin state instability, and has effect of preventing allergy.
In the step (4), the free sterol is more easily absorbed into the body, and the cell membrane growth is promoted from the inside of the body. The stigmasterol ester which can not be absorbed by organism remains in vitro, and a layer of protective film is formed on the surface of skin to lock the moisture of the skin, so that the moisturizing effect is enhanced.
In the step (5), the quercetin can eliminate in vivo free radicals, inhibit lipid peroxidation, reduce iron overload in organisms and reduce oxidative damage caused by iron overload. The zein-quercetin-lecithin compound enhances the thermal stability of the structure, improves lipid metabolism, provides sufficient nutrients such as water and oxygen for the skin, and repairs the damaged skin.
Compared with the prior art, the invention has the following beneficial effects:
(1) adding palmitic acid triglyceride. The fenugreek palmitic acid and glycerol are subjected to catalytic reaction, n-hexane is added for dissolving, super-strong base is used as a catalyst to synthesize small solid particles of palmitic acid triglyceride with adsorption effect, dead skin, dust and other dark substances on skin can be adsorbed by kneading massage to form large solid particles, and the large solid particles are taken away from an organism along with water flow to play a role in removing cutin. The residual palmitic acid triglyceride permeates into skin and reacts with lipoprotein lipase in the body to generate free fatty acid, the free fatty acid is absorbed by tissues and organs of the body to generate heat, the activity of acetylcholine in the body is activated, and the acetylcholine stimulates sweat glands to sweat and dispel damp and cold in the body through the allocation of sympathetic nerves. The trace residual alkalinity neutralizes the acid-base degree of the skin, improves the instability of the skin state, improves the photo-thermal stability and prevents allergy.
(2) Fenugreek sterol ester is added. Carrying out esterification reaction on the fenugreek sterol and the fatty acid to generate the fenugreek sterol ester. Due to the physical characteristics of hydrophobicity and lipophobicity, the fenugreek sterol ester is difficult to dissolve in water, has limited solubility in an oil phase, is poor in lipid solubility and is difficult to absorb by organisms. The fenugreek sterol fatty acid ester is generated through the catalysis of lipase in vivo, the fat solubility of the fenugreek sterol is improved, free sterol is generated through the hydrolysis of cholesterol esterase, the free sterol is easier to be absorbed by organisms, the cell activity is improved from the interior of the organisms, the cell membrane growth is promoted, and the skin is moisturized. The residual stigmasterol ester in vitro can not be absorbed by skin, so a layer of protective film is formed on the surface of skin, the moisture of the skin is locked, the moisturizing effect is enhanced, and the skin is moisturized and smooth.
(3) Adding zein: zein, quercetin and lecithin are stirred under the action of heating ethanol to generate a zein-quercetin-lecithin compound, and the zein is used as a bonding agent to connect the quercetin and the lecithin, so that the antioxidation of the lecithin is preserved, the allergy inhibiting effect of the quercetin is also preserved, and the water solubility of the quercetin is improved by utilizing the hydrophilicity of the phospholipid to cell membranes. After permeating into human body from pores, quercetin can eliminate free radicals in vivo, inhibit lipid peroxidation, reduce iron overload in organism, and reduce oxidation injury caused by iron overload. The zein-quercetin-lecithin compound enhances the thermal stability of the structure, improves lipid metabolism, and provides sufficient nutrients such as water and oxygen for the skin, thereby realizing smooth skin.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 shows hydroxyl radical scavenging values for example 1 and comparative examples 1, 2, 3, and 4.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A fenugreek seed extract body lotion comprises the following raw materials in parts by weight:
50-60 parts of fenugreek seeds, 30-50 parts of triglyceride palmitate, 40-60 parts of fenugreek palm oil, 40-60 parts of glycerol, 10-15 parts of fenugreek palmitic acid, 10-20 parts of fenugreek sterol, 10-20 parts of fatty acid, 5-10 parts of fenugreek sterol ester, 5-10 parts of fatty oil, 10-15 parts of zein, 10-15 parts of lecithin, 10-15 parts of quercetin, 5-10 parts of zein-quercetin-lecithin compound, 10-15 parts of sorbitol, 10-20 parts of absolute ethyl alcohol, 2-5 parts of preservative, 3-5 parts of essence and deionized water.
Preferably, the fenugreek palmitic acid is fenugreek fatty acid, after saponification, the soap solution is acidified by dilute sulfuric acid, the mixture is kept stand to obtain clear liquid, ether is used as an extracting agent to extract free fatty acid of the fenugreek, the fraction is obtained through reduced pressure distillation, and the fraction is cooled to obtain the fenugreek palmitic acid.
Preferably, the palmitic acid triglyceride and the derivatives thereof are prepared by carrying out catalytic reaction on fenugreek palmitic acid and glycerol, dissolving the reaction product in n-hexane, and then using superbase as a catalyst.
Preferably, the fenugreek sterol ester is prepared by esterification reaction of fenugreek sterol and fatty acid.
Preferably, the zein-quercetin-lecithin compound is prepared by mixing zein, quercetin and lecithin under magnetic stirring with heated ethanol.
Preferably, the preservative is one of formaldehyde-releasing preservatives such as sodium hydroxymethylglycinate, organic acid preservatives such as potassium sorbate, p-hydroxy and benzoate preservatives such as methylparaben, and the like.
In the technical scheme, the fenugreek palmitic acid is used as a softening agent and an emulsifying agent, and the saturated fatty acid is a main component in the fatty oil, can be used as a cleaning agent or a foaming agent and has the effects of moistening and cleaning the skin.
In the technical scheme, the palmitic acid triglyceride solid small particles can adsorb dark substances such as dead skin, dust and the like on the skin through rubbing and massage, and play a role in removing cutin. The trace residual alkalinity neutralizes the acid-base degree of skin, improves the instability of skin state, improves the photo-thermal stability, and has the effect of preventing allergy.
In the technical scheme, the free sterol is easier to be absorbed by the organism, and the cell membrane growth is promoted from the inside of the organism. The stigmasterol ester which can not be absorbed by organism remains in vitro, and a layer of protective film is formed on the surface of skin to lock the moisture of the skin, so that the moisturizing effect is enhanced.
In the technical scheme, the quercetin can eliminate free radicals in vivo, inhibit lipid peroxidation, reduce iron overload in organisms and reduce oxidative damage caused by iron overload. The zein-quercetin-lecithin compound enhances the thermal stability of the structure, improves lipid metabolism, and provides sufficient nutrients such as water and oxygen for the skin.
The invention provides a preparation method of fenugreek seed extract body milk, which comprises the following steps:
(1) extracting raw materials from fenugreek seeds:
extracting fatty acid of fenugreek, sterol of fenugreek, lecithin and quercetin;
(2) preparing fenugreek palmitic acid;
(3) preparing fenugreek palmitic acid triglyceride;
(4) preparing fenugreek sterol ester;
(5) preparing a zein-quercetin-lecithin compound;
(6) mixing sorbitol and glycerol, stirring, and adding into the substance obtained in step (2);
(7) adding the substance obtained in the step (3) into the step (5), and uniformly stirring;
(8) adding the substance obtained in the step (4) into the step (6), and uniformly stirring;
(9) adding the substance obtained in the step (7) into the step (8), and uniformly stirring;
(10) and (4) adding absolute ethyl alcohol, essence and preservative into the step (9), and mixing and stirring uniformly to obtain a finished product.
Preferably, the preparation method of the fenugreek palmitic acid in the step (2) comprises the following steps: adding a certain amount of fenugreek fatty acid, lacquer wax, a sodium hydroxide aqueous solution and a plurality of zeolites into a three-neck flask provided with a stirrer and a thermometer, electrically heating and boiling, refluxing and saponifying at 100-150 ℃, stopping heating after reacting for 3-5 h, acidifying the soap solution with dilute sulfuric acid after the saponified solution is cooled to 20-40 ℃ to enable the pH value of the soap solution to be about 4.0, then moving the soap solution into a separating funnel, standing and layering, separating out a clear solution layer, washing the residual solution with saturated salt water to enable the pH value to be neutral, extracting free fatty acid in the solution with diethyl ether as an extracting agent, distilling the extract at normal pressure, recovering the diethyl ether as a solvent for recycling, distilling in a reduced pressure distillation device, collecting fractions, and cooling to obtain the fenugreek palmitic acid.
Preferably, the preparation method of the fenugreek palmitic acid triglyceride in the step (3) comprises the following steps: glycerol and fenugreek palmitic acid are placed in a four-neck flask on a constant-temperature heating sleeve according to the mass ratio of 1:5, super-strong base is used as a catalyst, the reaction is carried out for 5-7 hours at the temperature of 150-250 ℃, nitrogen is continuously introduced as protective gas during the reaction, the temperature is reduced to room temperature after the reaction is finished, solid is separated out, n-hexane is added for dissolution, the super-strong base is removed through filtration, and the finished product is obtained through reduced pressure concentration.
Preferably, the preparation method of fenugreek sterol ester in the step (4) comprises the following steps: under the vacuum condition, excessive molten fatty acid is used as a reaction solvent, sodium bisulfate is used as a catalyst, the mass ratio of the fatty acid to the fenugreek sterol is 1.2:1, the reaction temperature is 100-200 ℃, and esterification reaction is directly carried out to generate fenugreek sterol ester.
Preferably, the method for preparing the zein-quercetin-lecithin compound in the step (5) comprises the following steps: dissolving 1.0g of zein in 100ml of 70% absolute ethyl alcohol, magnetically stirring for 1-2 h, adding lecithin to enable the mass ratio of the zein to the lecithin to be 2:3, magnetically stirring for 2-4 h, then adding 0.1g of quercetin into the mixed solution, magnetically stirring for 1-3 h, adding 20ml of zein-lecithin-quercetin ethanol solution into 60ml of deionized water, magnetically stirring for 20-40 min, evaporating ethanol remained in a dispersion body at 45 ℃ through a rotary evaporator, adjusting the dispersion body to PH40 by using a PH regulator, centrifuging for 20-30 min at 1500-2500 r/min to remove large particles and free quercetin, finally further analyzing the dispersion body, and freeze-drying for 40-60 h to obtain a finished product.
In the step (2), the fenugreek palmitic acid is used as a softening agent and an emulsifying agent, and the saturated fatty acid is a main component in the fatty oil and has the effects of moistening and cleaning the skin.
In the step (3), the palmitic acid triglyceride solid small particles can adsorb dark substances such as dead skin, dust and the like on the skin through rubbing and massage, and have the function of removing cutin. The trace residual alkalinity neutralizes the acid-base degree of skin, improves skin state instability, and has effect of preventing allergy.
In the step (4), the free sterol is more easily absorbed into the body, and the cell membrane growth is promoted from the inside of the body. The stigmasterol ester which can not be absorbed by organism remains in vitro, and a layer of protective film is formed on the surface of skin to lock the moisture of the skin, so that the moisturizing effect is enhanced.
In the step (5), the quercetin can eliminate in vivo free radicals, inhibit lipid peroxidation, reduce iron overload in organisms and reduce oxidative damage caused by iron overload. The zein-quercetin-lecithin compound enhances the thermal stability of the structure, improves lipid metabolism, provides sufficient nutrients such as water and oxygen for the skin, and repairs the damaged skin.
Example 1: a fenugreek seed extract body lotion I:
a fenugreek seed extract body lotion comprises the following components in parts by weight: 60 parts of fenugreek seeds, 40 parts of palmitic acid triglyceride, 50 parts of fenugreek palm oil, 40 parts of glycerol, 15 parts of fenugreek palmitic acid, 15 parts of fenugreek sterol, 15 parts of fenugreek fatty acid, 5 parts of fenugreek sterol ester, 5 parts of fatty oil, 15 parts of zein, 10 parts of lecithin, 15 parts of quercetin, 5 parts of zein-quercetin-lecithin compound, 10 parts of sorbitol, 10 parts of absolute ethyl alcohol, 2 parts of preservative, 3 parts of essence and deionized water.
The preparation method of the body lotion comprises the following steps:
(1) extracting raw materials from fenugreek seeds:
extracting fatty acid of fenugreek, sterol of fenugreek, lecithin and quercetin;
(2) the preparation method of the fenugreek palmitic acid comprises the following steps: weighing 10 parts of fenugreek fatty acid, adding lacquer wax, a sodium hydroxide aqueous solution and a plurality of zeolites with equal mass into a three-neck flask provided with a stirrer and a thermometer, electrically heating and boiling, refluxing and saponifying at 100 ℃, stopping heating after reacting for 3h, acidifying the soap solution with dilute sulfuric acid after the saponified solution is cooled to 20 ℃ to enable the pH value of the soap solution to be about 4.0, then moving the soap solution into a separating funnel, standing and layering the soap solution, separating a clear solution layer, washing the residual solution with saturated salt water to enable the pH value to be neutral, extracting free fatty acid in the soap solution by using ether as an extracting agent, distilling the extract at normal pressure, recovering the ether as a solvent for recycling, distilling in a reduced pressure distillation device, collecting fractions, and cooling to obtain the fenugreek palmitic acid.
(3) The preparation method of the fenugreek palmitic acid triglyceride comprises the following steps: weighing 10 parts of glycerol and 10 parts of fenugreek palmitic acid, placing the glycerol and the fenugreek palmitic acid in a four-neck flask on a constant-temperature heating sleeve according to a mass ratio of 1:5, reacting for 5 hours at 150 ℃ with super-strong base as a catalyst, continuously introducing nitrogen as protective gas during the reaction, cooling to room temperature after the reaction is finished, precipitating solids, adding n-hexane for dissolution, filtering to remove the super-strong base, and concentrating under reduced pressure to obtain a finished product.
(4) The preparation method of the fenugreek sterol ester comprises the following steps: weighing 5 parts of fenugreek sterol, taking excessive molten fatty acid as a reaction solvent and sodium bisulfate as a catalyst under a vacuum condition, wherein the mass ratio of the fatty acid to the fenugreek sterol is 1.2:1, and the esterification reaction is directly carried out at the reaction temperature of 100 ℃ to generate the fenugreek sterol ester.
(5) The preparation method of the zein-quercetin-lecithin compound comprises the following steps: dissolving 1.0g of zein in 100ml of 70% absolute ethyl alcohol, magnetically stirring for 1h, adding lecithin to enable the mass ratio of the zein to the lecithin to be 2:3, magnetically stirring for 2h, then adding 0.1g of quercetin into the mixed solution, magnetically stirring for 1h, adding 20ml of zein-lecithin-quercetin ethanol solution into 60ml of deionized water, magnetically stirring for 20min, evaporating ethanol remained in a dispersion body at 45 ℃ through a rotary evaporator, adjusting the dispersion body to PH 32 by using a PH regulator, centrifuging for 40min at 1500r/min to remove large particles and free quercetin, finally further analyzing the dispersion body, and freeze-drying for 40h to obtain a finished product.
(6) Mixing and stirring 5 parts of sorbitol and 5 parts of glycerol, and adding 10 parts by weight of the substance obtained in the step (2);
(7) adding 10 parts of the substance obtained in the step (3) into 2 parts of the substance obtained in the step (5), and stirring at the temperature of 40 ℃, the rotation speed of 400r/min and the time of 1 h;
(8) adding the substance obtained in the step (4) in the step (6), and stirring at 40 ℃ at the rotating speed of 400r/min for 2 h;
(9) adding 1 part of the substance obtained in the step (7) into the step (8), and stirring at 40 ℃ and 500r/min for 2 h;
(10) and (4) sequentially adding 4 parts by weight of absolute ethyl alcohol, 2 parts by weight of essence and 2 parts by weight of preservative into the step (9), mixing and stirring at the temperature of 30 ℃ and at the rotating speed of 100r/min, and thus obtaining a finished product.
Example 2: a second body lotion of fenugreek seed extract:
a fenugreek seed extract body lotion comprises the following components in parts by weight: 60 parts of fenugreek seeds, 50 parts of palmitic acid triglyceride, 60 parts of fenugreek palm oil, 40 parts of glycerol, 10 parts of fenugreek palmitic acid, 20 parts of fenugreek sterol, 10 parts of fenugreek fatty acid, 10 parts of fenugreek sterol ester, 10 parts of fatty oil, 10 parts of zein, 15 parts of lecithin, 10 parts of quercetin, 10 parts of zein-quercetin-lecithin compound, 15 parts of sorbitol, 20 parts of absolute ethyl alcohol, 5 parts of preservative, 5 parts of essence and deionized water.
The preparation method of the body lotion comprises the following steps:
(1) extracting raw materials from fenugreek seeds:
extracting fatty acid of fenugreek, sterol of fenugreek, lecithin and quercetin;
(2) the preparation method of the fenugreek palmitic acid comprises the following steps: weighing 15 parts of fenugreek fatty acid, adding lacquer wax, a sodium hydroxide aqueous solution and a plurality of zeolites with equal mass into a three-neck flask provided with a stirrer and a thermometer, electrically heating and boiling, refluxing and saponifying at 150 ℃, stopping heating after reacting for 5h, acidifying the soap solution with dilute sulfuric acid after the saponified solution is cooled to 40 ℃ to enable the pH value of the soap solution to be about 4.0, then moving the soap solution into a separating funnel, standing and layering the soap solution, separating a clear solution layer, washing the residual solution with saturated salt water to enable the pH value to be neutral, extracting free fatty acid in the soap solution by using ether as an extracting agent, distilling the extract at normal pressure, recovering the ether as a solvent for recycling, distilling in a reduced pressure distillation device, collecting fractions, and cooling to obtain the fenugreek palmitic acid.
(3) The preparation method of the fenugreek palmitic acid triglyceride comprises the following steps: weighing 20 parts of glycerol and 10 parts of fenugreek palmitic acid, placing the glycerol and the fenugreek palmitic acid in a four-neck flask on a constant-temperature heating sleeve according to a mass ratio of 1:5, reacting at 250 ℃ for 7 hours with super-strong base as a catalyst, continuously introducing nitrogen as protective gas during the reaction, cooling to room temperature after the reaction is finished, precipitating solids, adding n-hexane for dissolution, filtering to remove the super-strong base, and concentrating under reduced pressure to obtain a finished product.
(4) The preparation method of the fenugreek sterol ester comprises the following steps: weighing 10 parts of fenugreek sterol, taking excessive molten fatty acid as a reaction solvent and sodium bisulfate as a catalyst under a vacuum condition, wherein the mass ratio of the fatty acid to the fenugreek sterol is 1.2:1, and the esterification reaction is directly carried out at the reaction temperature of 200 ℃ to generate the fenugreek sterol ester.
(5) The preparation method of the zein-quercetin-lecithin compound comprises the following steps: dissolving 1.0g of zein in 100ml of 70% absolute ethyl alcohol, magnetically stirring for 2 hours, adding lecithin to enable the mass ratio of the zein to the lecithin to be 2:3, magnetically stirring for 4 hours, then adding 0.1g of quercetin into the mixed solution, magnetically stirring for 3 hours, adding 20ml of zein-lecithin-quercetin ethanol solution into 60ml of deionized water, magnetically stirring for 40 minutes, evaporating ethanol remained in a dispersion body at 45 ℃ by using a rotary evaporator, adjusting the dispersion body to be PH40 by using a PH regulator, centrifuging for 3530 minutes at 2500r/min to remove large particles and free quercetin, finally further analyzing the dispersion body, and freeze-drying for 60 hours to obtain a finished product.
(6) Mixing and stirring 10 parts of sorbitol and 10 parts of glycerol, and adding 10 parts by weight of the substance obtained in the step (2);
(7) adding 10 parts of the substance obtained in the step (3) into 2 parts of the substance obtained in the step (5), and stirring at the temperature of 60 ℃, the rotation speed of 600r/min and the time of 2 h;
(8) adding the substance obtained in the step (4) in the step (6), and stirring at 60 ℃ at the rotating speed of 600r/min for 4 h;
(9) adding 1 part of the substance obtained in the step (7) into the step (8), and stirring at the temperature of 60 ℃, at the rotating speed of 700r/min and for 4 hours;
(10) and (4) sequentially adding 10 parts by weight of absolute ethyl alcohol, 3 parts by weight of essence and 3 parts by weight of preservative into the step (9), mixing and stirring at the temperature of 40 ℃ and at the rotating speed of 200r/min, and thus obtaining a finished product.
Comparative example 1, ordinary body milk
The common body milk component comprises 20 parts of ethanol, 25 parts of glycerol, 15 parts of sorbitol, 5 parts of preservative, 5 parts of essence and deionized water in parts by weight.
The preparation method of the body lotion comprises the following steps:
(1) weighing 25 parts by weight of glycerol and 15 parts by weight of sorbitol, stirring and mixing, adding a proper amount of deionized water, stirring at the temperature of 60 ℃, at the rotating speed of 200r/min, and stirring for 10 min;
(2) and (2) sequentially adding 20 parts by weight of ethanol, 5 parts by weight of preservative and 5 parts by weight of essence into the substance obtained in the step (1), stirring until the ethanol, the preservative and the essence are completely dissolved, wherein the stirring temperature is 60 ℃, the rotating speed is 300r/min, and the stirring time is 2 hours, so as to obtain a finished product.
Comparative example 2 preparation of body milk of fenugreek extract without the addition of fenugreek palmitic acid triglycerides:
the formulation of the fenugreek extract body milk without the addition of fenugreek palmitic acid triglycerides was the same as in example 1. The preparation method of the body milk is different from that of example 1 only in that the preparation treatment of the step (3) is not performed, and the rest of the preparation steps are the same as those of example 1.
Comparative example 3 preparation of body milk of fenugreek extract without added fenugreek sterol ester:
the formulation of fenugreek extract body lotion without fenugreek sterol ester was the same as in example 1. The preparation method of the body lotion is different from that of example 1 only in that the preparation treatment of the step (4) is not performed, and the rest of the preparation steps are the same as those of example 1.
Comparative example 4 preparation of fenugreek extract body milk without zein-quercetin-lecithin addition:
the formulation of the fenugreek extract body milk without zein-quercetin-lecithin was the same as in example 1. The preparation method of the body milk is different from that of example 1 only in that the preparation treatment of the step (5) is not performed, and the rest of the preparation steps are the same as those of example 1.
Test example 1, the body milk of the fenugreek seed extract of the present invention was compared with the body milk of normal body, the body milk of the fenugreek extract without the addition of fenugreek palmitic acid triglyceride, the body milk of the fenugreek extract without the addition of fenugreek sterol ester, and the body milk of the fenugreek extract without the addition of zein-quercetin-lecithin:
1. test method
(1) 2.5ml of the fenugreek seed extract body lotion prepared in example 2 was taken, 2.5ml of 0.2mol/L phosphate buffer solution having a pH of 6.6 and 2.5ml of 1% potassium ferricyanide aqueous solution were added, the mixture was rapidly cooled after 50 minutes in water bath, 2.5ml of 10% trichloroacetic acid aqueous solution was added, the mixture was centrifuged at 3000r/min for 10 minutes, 5ml of supernatant was taken, 4ml of distilled water and 1ml of 0.1% ferric trichloride aqueous solution were added, the mixture was mixed well and then taken for 10 minutes, and the absorbance at 700nm was measured, and the larger the absorbance, the stronger the reducing power was indicated.
(2) 2.5ml of the ordinary body lotion prepared in comparative example 1 was taken, 2.5ml of a phosphate buffer solution containing 6.6 mol/LPH and 2.5ml of an aqueous solution containing 1% potassium ferricyanide were added, rapidly cooled after 20min in a 50 water bath, 2.5ml of an aqueous solution containing 10% trichloroacetic acid was added, centrifuged at 3000r/min for 10min, 5ml of the supernatant was taken, 4ml of distilled water and 1ml of an aqueous solution containing 0.1% ferric trichloride were added, mixed well and then 10min was carried out, and the absorbance value was measured at 700 nm.
(3) Taking 2.5ml of the fenugreek seed extract body lotion which is prepared in comparative example 2 and is not added with fenugreek palmitic acid triglyceride, adding 2.5ml of 0.2mol/LPH (6.6) phosphate buffer solution and 2.5ml of 1% potassium ferricyanide aqueous solution, rapidly cooling after 50-water bath for 20min, adding 2.5ml of 10% trichloroacetic acid aqueous solution, centrifuging at 3000r/min for 10min, taking 5ml of supernatant, adding 4ml of distilled water and 1ml of 0.1% ferric trichloride aqueous solution, uniformly mixing for 10min, and measuring the light absorption value at 700 nm.
(4) Taking 2.5ml of the fenugreek seed extract body lotion which is prepared in the comparative example 3 and is not added with fenugreek sterol ester, adding 2.5ml of 0.2mol/LPH (6.6) phosphate buffer solution and 2.5ml of 1% potassium ferricyanide aqueous solution, rapidly cooling after 50 water bath for 20min, adding 2.5ml of 10% trichloroacetic acid aqueous solution, centrifuging for 10min at 3000r/min, taking 5ml of supernatant, adding 4ml of distilled water and 1ml of 0.1% ferric trichloride aqueous solution, uniformly mixing for 10min, and measuring the light absorption value at 700 nm.
(5) Taking 2.5ml of the fenugreek seed extract body milk which is prepared in the comparative example 4 and is not added with the zein-quercetin-lecithin, adding 2.5ml of phosphate buffer solution with the mol/LPH being 6.6 and 2.5ml of aqueous solution containing 1% of potassium ferricyanide, rapidly cooling after 50-water bath for 20min, adding 2.5ml of aqueous solution containing 10% of trichloroacetic acid, centrifuging for 10min at 3000r/min, taking 5ml of supernate, adding 4ml of distilled water and 1ml of aqueous solution containing 0.1% of ferric trichloride, uniformly mixing for 10min, and measuring the light absorption value at 700 nm.
2. Results of the experiment
The reducing power of example 1 and comparative examples 1, 2, 3, 4 are shown in table 1.
Table 1 example 1 compares the reducing power of example 1 with that of comparative examples 1, 2, 3 and 4
Figure BDA0002817268100000161
The comparison in the table shows that, in the same time, the reduction capability of the common body milk, the body milk of the fenugreek extract without the addition of the fenugreek palmitic acid triglyceride, the body milk of the fenugreek extract without the addition of the fenugreek sterol ester and the body milk of the fenugreek extract without the addition of the zein-quercetin-lecithin are poorer than that of the body milk of the fenugreek seed extract, indicating that the body milk of the fenugreek seed extract has excellent capability of repairing damaged skin and keeping skin activity.
Test example 2 comparison of the ability of a fenugreek seed extract body milk of the present invention to scavenge hydroxyl radicals with normal body milk, fenugreek extract body milk without the addition of fenugreek palmitic acid triglyceride, fenugreek extract body milk without the addition of fenugreek sterol ester, and fenugreek extract body milk without the addition of zein-quercetin-lecithin:
1. test method
DPPH19.7mg was weighed accurately and made into 0.2 mmoleL DPPH solution by diluting to 250mL with 95% ethanol. Taking 2mL of the prepared DPPH solution, respectively adding 1mL of the fenugreek seed extract body milk prepared in example 1, the common body milk prepared in comparative example 1, the fenugreek extract body milk prepared in comparative example 2 without adding fenugreek palmitic acid triglyceride, the fenugreek extract body milk prepared in proportion 3 without adding fenugreek sterol ester, the fenugreek extract body milk prepared in proportion 4 without adding zein-quercetin-lecithin, supplementing to 4mL with 95% ethanol, mixing, standing for 30min, and measuring the absorbance A at the wavelength of 517nm1. Adding 2mL of 95% ethanol into the hydrolysate to replace the DPPH solution A2. Adding 1ml of ethanol as reference solution A into blank control solutioncThe clearance is calculated according to the formula: clearance (%) - (1- (A)1-A2)/Ac]*100
2. Test results
The scavenging hydroxyl radical values of example 1 and comparative examples 1, 2, 3, 4 are shown in figure 1.
As is apparent from the comparison in the table above, it is indicated that the general body milk prepared in comparative example 1, the body milk of the fenugreek extract without fenugreek palmitic acid triglyceride prepared in comparative example 2, the body milk of the fenugreek extract without fenugreek sterol ester prepared in proportion 3, and the body milk of the fenugreek extract without zein-quercetin-lecithin prepared in proportion 4 are respectively lower than the body milk of the fenugreek seed extract in the present invention in their ability to scavenge hydroxyl radicals, which indicates that the body milk of the fenugreek seed extract in the present invention has stronger oxidation resistance, inhibits lipid peroxidation in vivo, and reduces oxidative damage caused by iron overload.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The fenugreek seed extract body lotion is characterized by comprising the following raw materials in parts by weight: 50-60 parts of fenugreek seeds, 30-50 parts of triglyceride palmitate, 40-60 parts of fenugreek palm oil, 40-60 parts of glycerol, 10-15 parts of fenugreek palmitic acid, 10-20 parts of fenugreek sterol, 10-20 parts of fatty acid, 5-10 parts of fenugreek sterol ester, 5-10 parts of fatty oil, 10-15 parts of zein, 10-15 parts of lecithin, 10-15 parts of quercetin, 5-10 parts of zein-quercetin-lecithin compound, 10-15 parts of sorbitol, 10-20 parts of absolute ethyl alcohol, 2-5 parts of preservative, 3-5 parts of essence and deionized water.
2. The fenugreek seed extract body lotion according to claim 1, wherein: after the fenugreek palmitic acid is fatty acid of fenugreek, the soap solution is acidified by dilute sulphuric acid, the mixture is kept stand to obtain clear liquid, ether is used as an extracting agent to extract free fatty acid of the fenugreek, reduced pressure distillation is carried out to obtain fraction, and the fraction is cooled to obtain the fenugreek palmitic acid.
3. The fenugreek seed extract body lotion according to claim 1, wherein: the palmitic acid triglyceride is prepared by performing catalytic reaction on fenugreek palmitic acid and glycerol, dissolving in n-hexane, and using superbase as a catalyst.
4. The fenugreek seed extract body lotion according to claim 1, wherein: the fenugreek sterol ester is prepared by esterification reaction of fenugreek sterol and fatty acid.
5. The fenugreek seed extract body lotion according to claim 1, wherein: the zein-quercetin-lecithin compound is prepared by mixing zein, quercetin and lecithin under magnetic stirring with heated ethanol.
6. The fenugreek seed extract body lotion according to claim 5, wherein: the preservative is one of sodium hydroxymethyl glycinate, potassium sorbate and methylparaben.
7. A preparation method of fenugreek seed extract body lotion is characterized by comprising the following specific steps:
(1) extracting raw materials from fenugreek seeds:
extracting fatty acid of fenugreek, sterol of fenugreek, lecithin and quercetin;
(2) preparing fenugreek palmitic acid;
(3) preparing fenugreek palmitic acid triglyceride;
(4) preparing fenugreek sterol ester;
(5) preparing a zein-quercetin-lecithin compound;
(6) mixing sorbitol and glycerol, stirring, and adding into the substance obtained in step (2);
(7) adding the substance obtained in the step (3) into the step (5), and uniformly stirring;
(8) adding the substance obtained in the step (4) into the step (6), and uniformly stirring;
(9) adding the substance obtained in the step (7) into the step (8), and uniformly stirring;
(10) and (4) adding absolute ethyl alcohol, essence and preservative into the step (9), and mixing and stirring uniformly to obtain a finished product.
8. The method for preparing fenugreek seed extract body lotion according to claim 7, wherein the steps (2) and (3) of preparing fenugreek palmitic acid and palmitic triglyceride are both: adding a certain amount of fenugreek fatty acid, lacquer wax, a sodium hydroxide aqueous solution and a plurality of zeolites into a three-neck flask provided with a stirrer and a thermometer, electrically heating and boiling, refluxing and saponifying at 100-150 ℃, stopping heating after reacting for 3-5 h, acidifying the soap solution with dilute sulfuric acid after the saponified solution is cooled to 20-40 ℃ to enable the pH value of the soap solution to be about 4.0, then moving the soap solution into a separating funnel, standing and layering, separating out a clear solution layer, washing the residual solution with saturated salt water to enable the pH value to be neutral, extracting free fatty acid in the solution with diethyl ether as an extracting agent, distilling the extract at normal pressure, recovering the diethyl ether as a solvent for recycling, distilling in a reduced pressure distillation device, collecting fractions, and cooling to obtain the fenugreek palmitic acid; glycerol and fenugreek palmitic acid are placed in a four-neck flask on a constant-temperature heating sleeve according to the mass ratio of 1:5, super-strong base is used as a catalyst, the reaction is carried out for 5-7 hours at the temperature of 150-250 ℃, nitrogen is continuously introduced as protective gas during the reaction, the temperature is reduced to room temperature after the reaction is finished, solid is separated out, n-hexane is added for dissolution, the super-strong base is removed through filtration, and the finished product is obtained through reduced pressure concentration.
9. The method for preparing fenugreek seed extract body lotion according to claim 8, wherein the fenugreek sterol ester in the step (4) is prepared by: under the vacuum condition, excessive molten fatty acid is used as a reaction solvent, sodium bisulfate is used as a catalyst, the mass ratio of the fatty acid to the fenugreek sterol is 1.2:1, the reaction temperature is 100-200 ℃, and esterification reaction is directly carried out to generate fenugreek sterol ester.
10. The method for preparing a fenugreek seed extract body lotion according to claim 9, wherein the method for preparing the zein-quercetin-lecithin compound in the step (5) comprises: dissolving 1.0g of zein in 100ml of 70% absolute ethyl alcohol, magnetically stirring for 1-2 h, adding lecithin to enable the mass ratio of the zein to the lecithin to be 2:3, magnetically stirring for 2-4 h, then adding 0.1g of quercetin into the mixed solution, magnetically stirring for 1-3 h, adding 20ml of zein-lecithin-quercetin ethanol solution into 60ml of deionized water, magnetically stirring for 20-40 min, evaporating ethanol remained in a dispersion body at 45 ℃ through a rotary evaporator, adjusting the dispersion body to PH40 by using a PH regulator, centrifuging for 20-30 min at 1500-2500 r/min to remove large particles and free quercetin, finally further analyzing the dispersion body, and freeze-drying for 40-60 h to obtain a finished product.
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110755347A (en) * 2019-11-11 2020-02-07 中国科学院西北高原生物研究所 Skin moistening humectant and preparation method and application thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110755347A (en) * 2019-11-11 2020-02-07 中国科学院西北高原生物研究所 Skin moistening humectant and preparation method and application thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
季宇彬: "《抗癌中药药理与应用》", 28 February 1999, 黑龙江科学技术出版社 *
白首龙: ""1,3-二油酸-2-棕榈酸甘油三酯合成的工艺路线"", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 *
许青青等: ""植物甾醇酯的化学合成及其分离研究进展"", 《中国粮油学报》 *
邓卓丹等: ""玉米醇溶蛋白-卵磷脂-槲皮素纳米颗粒的制备与抗氧化特性"", 《食品与发酵工业》 *
陈先勇等: ""建始漆蜡的品质分析及棕榈酸的分离提纯"", 《湖北民族学院学报》 *

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