CN112345319B - 一种痰液样本的消化方法 - Google Patents
一种痰液样本的消化方法 Download PDFInfo
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- CN112345319B CN112345319B CN202011050880.5A CN202011050880A CN112345319B CN 112345319 B CN112345319 B CN 112345319B CN 202011050880 A CN202011050880 A CN 202011050880A CN 112345319 B CN112345319 B CN 112345319B
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- sputum
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- digesting
- sputum sample
- peptone
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- 206010036790 Productive cough Diseases 0.000 title claims abstract description 155
- 208000024794 sputum Diseases 0.000 title claims abstract description 155
- 210000003802 sputum Anatomy 0.000 title claims abstract description 153
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000029087 digestion Effects 0.000 title claims abstract description 30
- 230000001079 digestive effect Effects 0.000 claims abstract description 50
- 239000001888 Peptone Substances 0.000 claims abstract description 40
- 108010080698 Peptones Proteins 0.000 claims abstract description 40
- 235000019319 peptone Nutrition 0.000 claims abstract description 40
- 239000004094 surface-active agent Substances 0.000 claims abstract description 35
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 34
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 34
- 239000003223 protective agent Substances 0.000 claims abstract description 25
- 230000035939 shock Effects 0.000 claims abstract description 24
- 239000005416 organic matter Substances 0.000 claims abstract description 23
- 239000013530 defoamer Substances 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 42
- 235000018102 proteins Nutrition 0.000 claims description 33
- 239000002518 antifoaming agent Substances 0.000 claims description 28
- 230000009514 concussion Effects 0.000 claims description 25
- 239000002736 nonionic surfactant Substances 0.000 claims description 16
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 11
- 229920000053 polysorbate 80 Polymers 0.000 claims description 11
- 241001465754 Metazoa Species 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
- 150000001720 carbohydrates Chemical group 0.000 claims description 5
- -1 polyoxyethylene Polymers 0.000 claims description 5
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 229920001223 polyethylene glycol Polymers 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 229920001353 Dextrin Polymers 0.000 claims description 2
- 239000004375 Dextrin Substances 0.000 claims description 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
- 229940098773 bovine serum albumin Drugs 0.000 claims description 2
- 239000005018 casein Substances 0.000 claims description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000021240 caseins Nutrition 0.000 claims description 2
- 235000019425 dextrin Nutrition 0.000 claims description 2
- 229940093429 polyethylene glycol 6000 Drugs 0.000 claims description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 2
- 229920005862 polyol Polymers 0.000 claims 2
- 150000003077 polyols Chemical class 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 1
- 238000002133 sample digestion Methods 0.000 abstract description 6
- 238000011081 inoculation Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 32
- 239000000725 suspension Substances 0.000 description 32
- 150000001875 compounds Chemical class 0.000 description 24
- 206010062717 Increased upper airway secretion Diseases 0.000 description 9
- 238000002156 mixing Methods 0.000 description 9
- 208000026435 phlegm Diseases 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 239000003866 digestant Substances 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- JBDGDEWWOUBZPM-XYPYZODXSA-N ambroxol Chemical compound NC1=C(Br)C=C(Br)C=C1CN[C@@H]1CC[C@@H](O)CC1 JBDGDEWWOUBZPM-XYPYZODXSA-N 0.000 description 6
- 229960005174 ambroxol Drugs 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 239000002504 physiological saline solution Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000000721 bacterilogical effect Effects 0.000 description 4
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- 239000002609 medium Substances 0.000 description 4
- 150000002894 organic compounds Chemical class 0.000 description 4
- 239000011368 organic material Substances 0.000 description 3
- 230000010355 oscillation Effects 0.000 description 3
- 150000005846 sugar alcohols Polymers 0.000 description 3
- 241000605447 Anemarrhena Species 0.000 description 2
- 229930191283 anemarrhena Natural products 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
- 241000588655 Moraxella catarrhalis Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 108010027597 alpha-chymotrypsin Proteins 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 208000003265 stomatitis Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011050880.5A CN112345319B (zh) | 2020-09-29 | 2020-09-29 | 一种痰液样本的消化方法 |
| PCT/CN2021/120824 WO2022068751A1 (fr) | 2020-09-29 | 2021-09-27 | Procédé de digestion d'échantillon d'expectoration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011050880.5A CN112345319B (zh) | 2020-09-29 | 2020-09-29 | 一种痰液样本的消化方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112345319A CN112345319A (zh) | 2021-02-09 |
| CN112345319B true CN112345319B (zh) | 2024-05-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011050880.5A Active CN112345319B (zh) | 2020-09-29 | 2020-09-29 | 一种痰液样本的消化方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112345319B (fr) |
| WO (1) | WO2022068751A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345319B (zh) * | 2020-09-29 | 2024-05-17 | 郑州安图生物工程股份有限公司 | 一种痰液样本的消化方法 |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4053363A (en) * | 1976-06-28 | 1977-10-11 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Sputum analysis method |
| EP0571203A1 (fr) * | 1992-05-22 | 1993-11-24 | Becton, Dickinson and Company | Milieu pour le transport d'un spécimen de micro-organismes contenant un agent qui lyse les cellules blanches de sang |
| CN104232621A (zh) * | 2014-09-23 | 2014-12-24 | 宁波有成生物医药科技有限公司 | 一种从痰液中提取核酸的试剂盒及提取方法 |
| CN104254597A (zh) * | 2012-02-29 | 2014-12-31 | Bd公司 | 从阳性血培养中隔离活性微生物的流程与配方 |
| CN104531659A (zh) * | 2014-12-16 | 2015-04-22 | 四川新健康成生物股份有限公司 | 胰蛋白酶消化液、制备方法及即用型痰消化装置 |
| CN105506085A (zh) * | 2015-12-28 | 2016-04-20 | 北京澳利文生物科技有限公司 | 一种粘稠液状标本的处理方法 |
| CN107667168A (zh) * | 2015-05-27 | 2018-02-06 | 凯杰有限公司 | 用于破碎组织材料的组合物和方法 |
| WO2019222862A1 (fr) * | 2018-05-25 | 2019-11-28 | Qvella Corporation | Procédés et compositions destinées à la lyse sélective de cellules sanguines et à la séparation de cellules microbiennes |
| CN111238893A (zh) * | 2020-01-19 | 2020-06-05 | 湖北泰康医疗设备有限公司 | 一种用于检查肺癌的体液细胞的提取方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101158647A (zh) * | 2007-11-13 | 2008-04-09 | 天津市疾病预防控制中心 | 嗜肺军团菌生化反应的鉴定方法 |
| CN103146799A (zh) * | 2013-03-28 | 2013-06-12 | 中国人民解放军第三军医大学 | 微生态检测用试剂盒 |
| CN104561230B (zh) * | 2015-01-22 | 2015-09-02 | 四川金域医学检验中心有限公司 | 一种医学检测痰液标本匀质化方法及试剂的制备 |
| WO2017088169A1 (fr) * | 2015-11-27 | 2017-06-01 | Coyote Bioscience Co., Ltd. | Procédés et systèmes d'amplification d'acides nucléiques |
| CN206531717U (zh) * | 2017-02-23 | 2017-09-29 | 郑州安图生物工程股份有限公司 | 自动添加痰消化液的加样系统 |
| CN112345319B (zh) * | 2020-09-29 | 2024-05-17 | 郑州安图生物工程股份有限公司 | 一种痰液样本的消化方法 |
-
2020
- 2020-09-29 CN CN202011050880.5A patent/CN112345319B/zh active Active
-
2021
- 2021-09-27 WO PCT/CN2021/120824 patent/WO2022068751A1/fr active Application Filing
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4053363A (en) * | 1976-06-28 | 1977-10-11 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Sputum analysis method |
| EP0571203A1 (fr) * | 1992-05-22 | 1993-11-24 | Becton, Dickinson and Company | Milieu pour le transport d'un spécimen de micro-organismes contenant un agent qui lyse les cellules blanches de sang |
| CN104254597A (zh) * | 2012-02-29 | 2014-12-31 | Bd公司 | 从阳性血培养中隔离活性微生物的流程与配方 |
| CN104232621A (zh) * | 2014-09-23 | 2014-12-24 | 宁波有成生物医药科技有限公司 | 一种从痰液中提取核酸的试剂盒及提取方法 |
| CN104531659A (zh) * | 2014-12-16 | 2015-04-22 | 四川新健康成生物股份有限公司 | 胰蛋白酶消化液、制备方法及即用型痰消化装置 |
| CN107667168A (zh) * | 2015-05-27 | 2018-02-06 | 凯杰有限公司 | 用于破碎组织材料的组合物和方法 |
| CN105506085A (zh) * | 2015-12-28 | 2016-04-20 | 北京澳利文生物科技有限公司 | 一种粘稠液状标本的处理方法 |
| WO2019222862A1 (fr) * | 2018-05-25 | 2019-11-28 | Qvella Corporation | Procédés et compositions destinées à la lyse sélective de cellules sanguines et à la séparation de cellules microbiennes |
| CN111238893A (zh) * | 2020-01-19 | 2020-06-05 | 湖北泰康医疗设备有限公司 | 一种用于检查肺癌的体液细胞的提取方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112345319A (zh) | 2021-02-09 |
| WO2022068751A1 (fr) | 2022-04-07 |
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