CN112337514A - Micro-fluidic chip - Google Patents
Micro-fluidic chip Download PDFInfo
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- CN112337514A CN112337514A CN201910735572.7A CN201910735572A CN112337514A CN 112337514 A CN112337514 A CN 112337514A CN 201910735572 A CN201910735572 A CN 201910735572A CN 112337514 A CN112337514 A CN 112337514A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
Abstract
The invention relates to a micro-fluidic chip, which comprises a cover plate layer and a substrate layer, wherein the cover plate layer and the substrate layer are combined into a whole through an adhesive layer, the substrate layer comprises an inlet filtering section, a screening section, a channel section and a waste liquid groove section, a sample inlet is arranged on the cover plate layer and is communicated with the inlet filtering section, a sample liquid enters the inlet filtering section through the sample inlet to filter impurities and then enters the screening section, the sample liquid is separated into a plurality of screening liquids containing particles with different particle sizes through the screening section, and the screening liquids enter a waste liquid groove of the waste liquid groove section through the channel section. The invention adopts the preposed filtering structure and the screening structure to filter impurities in the sample liquid to be detected, separates the sample liquid into a plurality of screening liquids containing particles with different particle sizes, and carries out synchronous detection, and has the characteristics of good detection effect and high efficiency.
Description
Technical Field
The invention relates to a micro-fluidic chip, in particular to a high-efficiency micro-fluidic chip, and belongs to the field of micro-fluidic chips.
Background
Microfluidics is a technology of a system that processes or manipulates minute fluids of nanoliter to attoliter volumes using microchannels of tens to hundreds of micrometers in size. Microfluidics has the following characteristics: the low-energy consumption device has the advantages of small capacity, nano-liter, pico-liter and fly-liter levels, and small occupied volume; the microfluidics is used for controlling the fluid under the microscale and is a multidisciplinary cross technology of engineering, physics, chemistry, micromachining, bioengineering and the like; and the spatial characteristic dimension range of the microfluidic research is between 1 micron and 1 millimeter. Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as Lab-on-a-chips (labs). One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. Laminar flow is a flow condition of a fluid, i.e. laminar flow, and the fluid exhibits laminar flow when flowing at a low speed in a pipe, and its mass points move smoothly and linearly in a direction parallel to the pipe axis, and the flow speed of the fluid is maximum at the center of the pipe and minimum at the near wall. The liquid droplets are fine liquid particles which can settle under static conditions and remain in suspension under turbulent conditions, and have a primary particle size of 200 μm or less. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods.
Disclosure of Invention
The invention discloses a novel scheme, which adopts a preposed filtering structure and a screening structure to filter impurities in a sample liquid to be detected, separates the sample liquid into a plurality of screening liquids containing particles with different particle sizes, and performs synchronous detection, thereby solving the problems that the impurities in the sample liquid cannot be removed by the prior similar products and the detection efficiency is low.
The microfluidic chip comprises a cover plate layer and a substrate layer, wherein the cover plate layer and the substrate layer are combined into a whole through an adhesive layer to form the microfluidic chip, the substrate layer comprises an inlet filtering section, a screening section, a channel section and a waste liquid groove section, a sample inlet is formed in the cover plate layer and is communicated with the inlet filtering section, sample liquid enters the inlet filtering section through the sample inlet, is filtered from impurities and then enters the screening section, the sample liquid is separated into a plurality of screening liquids containing particles with different particle sizes through the screening section, and the screening liquids enter a waste liquid groove of the waste liquid groove section through the channel section.
Furthermore, the inlet filtering section comprises a filtering tank, a filtering membrane A is arranged in the filtering tank, the filtering membrane A divides the interior of the filtering tank into an upper liquid inlet chamber and a lower filtrate chamber, the filtrate chamber is communicated with an upper inlet of the screening section through a filtrate channel A, sample liquid entering the liquid inlet chamber enters the filtrate chamber through the filtering membrane A to form filtrate, and the filtrate enters the screening section through the filtrate channel A under the pressure action of the sample liquid in the upper liquid inlet chamber.
Furthermore, a filter membrane B is arranged in the filter liquid chamber, the filter membrane B divides the interior of the filter liquid chamber into an upper primary filter chamber and a lower fine filter chamber, the fine filter chamber is communicated with an upper inlet of the screening section through a filter liquid channel A, filter liquid entering the primary filter chamber enters the fine filter chamber through the filter membrane B to form fine filter liquid, and the fine filter liquid enters the screening section through the filter liquid channel A under the pressure action of primary filter liquid in the upper primary filter chamber.
Furthermore, the screening section of this scheme includes the filtrate entry on central upper portion, the filtrate chamber of central authorities, central authorities' outlying screening room, is equipped with a plurality of one-level screening boards that are annular equidistance and arrange between filtrate chamber and the screening room, and the indoor filtrate of filtrate gets into the screening room through the screening clearance between adjacent one-level screening board and forms one-level screening liquid, and the indoor filtrate of filtrate gets into the passageway section through filtrate passageway B with filtrate chamber bottom intercommunication, and the indoor one-level screening liquid of screening gets into the passageway section through the one-level screening liquid passageway with screening room top intercommunication.
Furthermore, a plurality of second grade screening plates which are arranged in an annular equidistance mode are arranged in the screening chamber, the screening chamber is divided into a first grade screening chamber with an inner ring and a second grade screening chamber with an outer ring by the aid of the second grade screening plates, filtrate in the filtrate chamber enters the first grade screening chamber through screening gaps between adjacent first grade screening plates to form first grade screening liquid, the first grade screening liquid in the first grade screening chamber enters the channel section through a first grade screening liquid channel communicated with the top of the first grade screening chamber, the first grade screening liquid in the first grade screening chamber enters the second grade screening chamber through screening gaps between adjacent second grade screening plates to form second grade screening liquid, and the second grade screening liquid in the second grade screening chamber enters the channel section through a second grade screening liquid channel communicated with the top of the second grade screening chamber.
Still further, the passageway section of this scheme includes filtrating test channel, one-level screening liquid test channel, second grade screening liquid test channel, filtrating test channel's entry and filtrating channel B intercommunication, filtrating test channel's export and waste liquid groove intercommunication, one-level screening liquid test channel's entry and one-level screening liquid channel intercommunication, one-level screening liquid channel's export and waste liquid groove intercommunication, second grade screening liquid test channel's entry and second grade screening liquid channel intercommunication, second grade screening liquid channel's export and waste liquid groove intercommunication.
Still further, the filtrating test channel, the first grade sifting liquid test channel, the second grade sifting liquid test channel of this scheme are the test channel with the same structure, the test channel includes the first grade test section, the second grade test section, the first grade test section is the channel of zigzag extension, the export of the first grade test section communicates with the entry of the second grade test section, the export of the second grade test section communicates with the waste liquid groove.
Furthermore, the waste liquid groove of the scheme is internally provided with a waste liquid absorbing substance.
Further, the waste liquid absorbent of the present embodiment is a water absorbent sponge.
The micro-fluidic chip disclosed by the invention filters impurities in the sample liquid to be detected by adopting the preposed filtering structure and screening structure, separates the sample liquid into various screening liquids containing particles with different particle sizes, and performs synchronous detection, and has the characteristics of good detection effect and high efficiency.
Drawings
Fig. 1 is a schematic top view of a microfluidic chip.
Fig. 2 is a schematic front view of a microfluidic chip.
FIG. 3 is a schematic cross-sectional view of an example one of the inlet filter segments.
FIG. 4 is a schematic cross-sectional view of example two of the inlet filter segment.
Figure 5 is a schematic cross-sectional view of an example one of the screening sections.
Figure 6 is a schematic cross-sectional view of example two of the screening section.
Wherein 100 is a cover sheet layer, 110 is a sample inlet, 200 is a substrate layer, 210 is an inlet filtration section, 211 is a filter membrane a, 212 is a filtrate channel a, 213 is a filter membrane B, 220 is a sieving section, 221 is a primary sieving plate, 222 is a filtrate channel B, 223 is a primary sieving liquid channel, 224 is a secondary sieving plate, 225 is a secondary sieving liquid channel, 230 is a channel section, 231 is a primary detection section, 232 is a secondary detection section, 240 is a waste liquid groove section, 241 is a waste liquid groove, and 300 is an adhesive layer.
Detailed Description
As shown in fig. 1-2, the microfluidic chip of the present invention includes a cover layer and a substrate layer, the cover layer is integrated with the substrate layer by an adhesive layer to form a microfluidic chip, the substrate layer includes an inlet filter section, a sieve section, a channel section, and a waste liquid tank section, the cover layer is provided with a sample inlet, the sample inlet is communicated with the inlet filter section, a sample liquid enters the inlet filter section through the sample inlet to filter impurities, the sample liquid enters the sieve section through the sieve section, the sample liquid is separated into a plurality of sieve liquids containing particles with different particle sizes through the sieve section, and the sieve liquid enters a waste liquid tank of the waste liquid tank section through the channel section. Above-mentioned scheme adopts leading filtration, screening structure filtering to examine the impurity in the sample liquid, separates into the multiple screening liquid that contains different particle diameter granule with sample liquid, carries out synchronous detection, specifically is that sample liquid filters harmful particle wherein after getting into the entry fillter section, and the filtrating gets into the sample liquid that is separated into to have different particle diameter granule after the segment of screening, can detect to the multiple sample liquid that separates simultaneously in step, has improved the effect and the efficiency that detect by a wide margin.
In order to realize the function of the inlet filtering section, as shown in fig. 3, the inlet filtering section of the present scheme includes a filtering tank, a filtering membrane a is arranged in the filtering tank, the filtering membrane a divides the interior of the filtering tank into an upper liquid inlet chamber and a lower filtrate chamber, the filtrate chamber is communicated with an upper inlet of the screening section through a filtrate channel a, a sample liquid entering the liquid inlet chamber enters the filtrate chamber through the filtering membrane a to form a filtrate, and the filtrate enters the screening section through the filtrate channel a under the pressure action of the sample liquid in the upper liquid inlet chamber. Based on the scheme, in order to further filter harmful particles in the sample liquid, as shown in fig. 4, a filter membrane B is arranged in the filtrate chamber of the scheme, the filter membrane B divides the interior of the filtrate chamber into an upper primary filter chamber and a lower fine filter chamber, the fine filter chamber is communicated with an upper inlet of the screening section through a filter channel a, the filter liquid entering the primary filter chamber enters the fine filter chamber through the filter membrane B to form fine filter liquid, and the fine filter liquid enters the screening section through the filter channel a under the pressure action of the primary filter liquid in the upper primary filter chamber. Above scheme adopts two-layer filterable scheme, improves the filter effect, but this scheme is not restricted to two-layer filtration, can also be three-layer and above filtration scheme.
In order to realize the function of the screening section, as shown in fig. 5, the screening section of the scheme comprises a filtrate inlet at the upper part of the center, a filtrate chamber at the center and a screening chamber at the periphery of the center, wherein a plurality of primary screening plates which are annularly and equidistantly arranged are arranged between the filtrate chamber and the screening chamber, filtrate in the filtrate chamber enters the screening chamber through a screening gap between adjacent primary screening plates to form primary screening liquid, the filtrate in the filtrate chamber enters the channel section through a filtrate channel B communicated with the bottom of the filtrate chamber, and the primary screening liquid in the screening chamber enters the channel section through a primary screening liquid channel communicated with the top of the screening chamber. Based on the above scheme, in order to sieve the sample liquid that more have different particle size granule, divide the classification into separately, as shown in fig. 6, be equipped with a plurality of second grade screening boards that are annular equidistance and arrange in the screening room of this scheme, above-mentioned a plurality of second grade screening boards divide the screening room internal partitioning into the first order screening room of inner ring, the second grade screening room of outer loop, the interior filtrating of filtrate room gets into the first order screening room through the screening clearance between adjacent first order screening inter-plate and forms first order screening liquid, the first order screening liquid in the first order screening room gets into the passageway section through the first order screening liquid passageway with first order screening room top intercommunication, the first order screening liquid in the first order screening room gets into the second order screening room through the screening clearance between adjacent second grade screening inter-plate and forms second order screening liquid, the interior second order screening liquid of second order screening room gets into the passageway section through the second order screening liquid passageway with. The scheme adopts a two-stage separation scheme, so that the detection efficiency is improved, but the scheme is not limited to two-stage separation and can also be a three-stage or more separation scheme.
Based on above scheme, in order to realize the function of passageway section, cooperation multiple sample liquid synchronous detection, as shown in fig. 1, the passageway section of this scheme includes filtrating detection channel, the liquid detection channel of one-level screening, the liquid detection channel of second grade screening, the entry and the filtrating channel B intercommunication of filtrating detection channel, the export and the waste liquid groove intercommunication of filtrating detection channel, the entry and the liquid channel intercommunication of one-level screening of liquid detection channel, the export and the waste liquid groove intercommunication of one-level screening liquid channel, the entry and the liquid channel intercommunication of second grade screening of liquid detection channel, the export and the waste liquid groove intercommunication of second grade screening liquid channel. Further, in order to realize the detection function of passageway section, the filtrating test passage of this scheme, one-level screening liquid test passage, second grade screening liquid test passage are the test passage that the structure is the same, and test passage includes one-level test section, second grade test section, and one-level test section is the passageway of zigzag extension, and the export of one-level test section and the entry intercommunication of second grade test section, the export and the waste liquid groove intercommunication of second grade test section. The scheme of the multi-section detection channel further improves the detection effect.
In order to realize the liquid storage function of the waste liquid tank and avoid the overflow of waste liquid, the waste liquid tank is internally provided with a waste liquid absorbing substance. Further, the waste liquid absorbing substance of the present embodiment is a water absorbing sponge.
The microfluidic chip of the present solution is not limited to the disclosure in the specific embodiment, the technical solutions presented in the examples can be extended based on the understanding of the person skilled in the art, and the simple alternatives made by the person skilled in the art according to the present solution in combination with the common general knowledge also belong to the scope of the present solution.
Claims (9)
1. The microfluidic chip is characterized by comprising a cover plate layer and a substrate layer, wherein the cover plate layer and the substrate layer are combined into a whole through an adhesive layer, the substrate layer comprises an inlet filtering section, a screening section, a channel section and a waste liquid groove section, a sample inlet is formed in the cover plate layer and is communicated with the inlet filtering section, a sample liquid enters the inlet filtering section through the sample inlet, impurities are filtered out of the sample liquid and then enters the screening section, the sample liquid is separated into a plurality of screening liquids containing particles with different particle sizes through the screening section, and the screening liquids enter the waste liquid groove of the waste liquid groove section through the channel section.
2. The microfluidic chip according to claim 1, wherein the inlet filtering section comprises a filtering tank, a filtering membrane a is disposed in the filtering tank, the filtering membrane a divides the inside of the filtering tank into an upper liquid inlet chamber and a lower filtrate chamber, the filtrate chamber is communicated with an upper inlet of the sieving section through a filtrate channel a, a sample liquid entering the liquid inlet chamber enters the filtrate chamber through the filtering membrane a to form a filtrate, and the filtrate enters the sieving section through the filtrate channel a under the pressure of the sample liquid in the upper liquid inlet chamber.
3. The microfluidic chip according to claim 2, wherein a filter membrane B is disposed in the filtrate chamber, the filter membrane B divides the interior of the filtrate chamber into an upper primary filter chamber and a lower fine filter chamber, the fine filter chamber is communicated with the upper inlet of the screen segment through the filtrate channel a, the filtrate entering the primary filter chamber enters the fine filter chamber through the filter membrane B to form a fine filter, and the fine filter enters the screen segment through the filtrate channel a under the pressure of the primary filtrate in the upper primary filter chamber.
4. The microfluidic chip according to claim 1, wherein the sieving section comprises a filtrate inlet at the upper part of the center, a filtrate chamber at the center, and a sieving chamber at the periphery of the center, a plurality of primary sieving plates are arranged between the filtrate chamber and the sieving chamber in an annular and equidistant manner, the filtrate in the filtrate chamber enters the sieving chamber through a sieving gap between the adjacent primary sieving plates to form primary sieving liquid, the filtrate in the filtrate chamber enters the channel section through a filtrate channel B communicated with the bottom of the filtrate chamber, and the primary sieving liquid in the sieving chamber enters the channel section through a primary sieving liquid channel communicated with the top of the sieving chamber.
5. The microfluidic chip according to claim 4, wherein a plurality of secondary sieving plates are arranged in the sieving chamber at equal intervals in a ring shape, the screening chamber is divided into a first-stage screening chamber with an inner ring and a second-stage screening chamber with an outer ring by the secondary screening plates, the filtrate in the filtrate chamber enters the primary screening chamber through the screening gap between the adjacent primary screening plates to form primary screening liquid, the primary screening liquid in the primary screening chamber enters the channel section through a primary screening liquid channel communicated with the top of the primary screening chamber, the primary screening liquid in the primary screening chamber enters the secondary screening chamber through the screening gaps between the adjacent secondary screening plates to form secondary screening liquid, and the secondary screening liquid in the secondary screening chamber enters the channel section through a secondary screening liquid channel communicated with the top of the secondary screening chamber.
6. The microfluidic chip according to claim 5, wherein the channel segment comprises a filtrate detection channel, a primary screening liquid detection channel, and a secondary screening liquid detection channel, wherein an inlet of the filtrate detection channel is communicated with the filtrate channel B, an outlet of the filtrate detection channel is communicated with the waste liquid tank, an inlet of the primary screening liquid detection channel is communicated with the primary screening liquid channel, an outlet of the primary screening liquid channel is communicated with the waste liquid tank, an inlet of the secondary screening liquid detection channel is communicated with the secondary screening liquid channel, and an outlet of the secondary screening liquid channel is communicated with the waste liquid tank.
7. The microfluidic chip according to claim 6, wherein the filtrate detection channel, the primary screening liquid detection channel, and the secondary screening liquid detection channel are detection channels having the same structure, and the detection channels comprise a primary detection section and a secondary detection section, the primary detection section is a zigzag extending channel, an outlet of the primary detection section is communicated with an inlet of the secondary detection section, and an outlet of the secondary detection section is communicated with the waste liquid tank.
8. The microfluidic chip according to claim 1, wherein the waste liquid tank contains a waste liquid absorbent.
9. The microfluidic chip according to claim 8, wherein the waste fluid absorbent is a water absorbent sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910735572.7A CN112337514A (en) | 2019-08-09 | 2019-08-09 | Micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910735572.7A CN112337514A (en) | 2019-08-09 | 2019-08-09 | Micro-fluidic chip |
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| CN112337514A true CN112337514A (en) | 2021-02-09 |
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| CN201910735572.7A Pending CN112337514A (en) | 2019-08-09 | 2019-08-09 | Micro-fluidic chip |
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| CN108896751A (en) * | 2018-05-24 | 2018-11-27 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
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2019
- 2019-08-09 CN CN201910735572.7A patent/CN112337514A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20170128941A1 (en) * | 2013-09-05 | 2017-05-11 | Bio-Rad Laboratories, Inc. | On-demand particle dispensing system |
| CN106190774A (en) * | 2016-07-22 | 2016-12-07 | 深圳睿思生命科技有限公司 | For capturing the micro-fluidic chip of circulating tumor cell and capture thereof and authentication method |
| CN106770085A (en) * | 2016-11-21 | 2017-05-31 | 大连海事大学 | A kind of ballast water for ship device for fast detecting and method based on micro-fluidic chip |
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Effective date of registration: 20210816 Address after: No.66210, 3rd floor, Pudong Free Trade Zone, Shanghai, China Applicant after: Shanghai Renxin Biotechnology Co.,Ltd. Address before: 201203 Pudong New Area, Shanghai, China (Shanghai) free trade trial area, No. 3, 1 1, Fang Chun road. Applicant before: SHANGHAI RENJING BIOLOGICAL TECHNOLOGY Co.,Ltd. |
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