CN112335716B - Method for rapidly picking otoliths of flounder larvae - Google Patents
Method for rapidly picking otoliths of flounder larvae Download PDFInfo
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- CN112335716B CN112335716B CN202011207239.8A CN202011207239A CN112335716B CN 112335716 B CN112335716 B CN 112335716B CN 202011207239 A CN202011207239 A CN 202011207239A CN 112335716 B CN112335716 B CN 112335716B
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- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22B—SLAUGHTERING
- A22B3/00—Slaughtering or stunning
- A22B3/08—Slaughtering or stunning for poultry or fish, e.g. slaughtering pliers, slaughtering shears
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention provides a method for quickly picking otoliths of flounder larvae, which aims at the structural characteristics of the flounder larvae, cuts the flounder larvae backwards from the middle position of two eyes to the rear edge of a gill cover from an anastomosis section, then cuts off the fish head from the top of the fish head along the rear edge of the gill cover in the direction vertical to the spine, and separates the head of the flounder larvae from the body to obtain the head; then, the skull of the head of the fish is cut off to expose the brain tissue of the fish, the brain tissue is turned forward from the cut part at the back to expose the sagittaria pinnata in the spherical sac, the sagittaria pinnata is firstly picked out, and then the sagittaria pinnata and the micro-tragus pinnata are picked out. The method provided by the invention can quickly, accurately and completely pick out the three pairs of otoliths of the flounder juvenile fish, save the time for picking the otoliths of the flounder juvenile fish, shorten the picking period and have wide application prospect.
Description
Technical Field
The invention belongs to the technical field of biological sample preparation, and particularly relates to a method for quickly picking otoliths of flounder larvae.
Background
Teleostoma species have 1 pair of each of a trabecterite (Lapilli), a Sagittae (Sagittae) and an Asteriscus (Asteriscus), are respectively positioned in an oval sac, a sacculus and an auditory kettle of an inner ear, and play important roles in fish balance and auditory sense. With the development of science and technology in recent years, researchers can effectively reflect the environmental characteristics of fish living water areas and the difference of fish populations by analyzing trace elements deposited in otoliths, provide references for researches on environmental changes, fish growth and development, living environments, resource distribution and the like of fishes in the whole life history, provide important basic information for researching the age, the growth and the migration of the fishes, and have important reference values in the research on fish biology and resource assessment.
Picking up intact fish otoliths is a prerequisite for the development of the above otolith-related research. At present, most of otoliths are picked up by adopting dissecting scissors along the direction of the spinal vertebrae of a fish body to cut the skull bone of the head of the fish body, take out brain tissues, then take out organic matters and colloid below the back of the brain and search for the otoliths. A method for rapidly picking sagitta from fusiform and flat fish (CN 105028281A): the fusiform fish turns over gills, and punctures the inner ear ball sac of the fish by using forceps to obtain sagittaria; the fish in lateral flat shape is obtained by cutting the back part obliquely above the fish eye and picking the sagittaria from the inner ear sacculus of the fish. The method for picking the fish otoliths in the prior art is not suitable for flounder juvenile fishes and mainly has the following problems: 1. according to the conventional method for picking the otolith, the organic matters and the colloid of the brain are more, confusion is easy to cause, and the process for picking the otolith is complex and time-consuming. During extraction of otoliths and micro otoliths, otoliths may be lost as the gum moves. 2. The method for picking the fusiform fish otolith can quickly pick the sagittaria sagittifolia, but the search for the micro otolith and the stellerite is difficult. 3. The side flat fish is opened on the back above the eye slant, which is easy to cause the cracking of the ear stones of flounder and flounder, and the integrity of the ear stones is difficult to ensure. 4. The conventional picking method is easy to cause the loss of otoliths. The flounder juvenile fish is small in size, small otoliths are not easy to find in the process of picking the otoliths, and two eyes of the flounder juvenile fish are positioned at one side of the head.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for quickly and completely picking three pairs of otoliths of flounder juvenile fishes.
The invention aims to provide a method for quickly picking otoliths of flounder larvae.
The invention provides a method for rapidly picking otoliths of flounder larvae, which comprises the following steps:
(1) separating the head of flounder juvenile fish from the body to obtain the head of flounder juvenile fish;
(2) placing the head of the flounder juvenile fish upright, and then cutting off the skull to obtain the head of the treated flounder juvenile fish;
(3) placing the head of the treated flounder juvenile fish under a microscope to expose the brain tissue of the fish;
(4) exposing the sagittaria trabeculosa in the spherical sac, taking out the stellerite, and then taking out the sagittaria trabeculosa and the micro-otolith to obtain three pairs of complete otoliths.
The invention provides a mode for rapidly picking otolith of flounder larvae, which comprises the steps of firstly separating the head of flounder larvae from a body according to the characteristics of the flounder larvae per se to obtain the head, then placing the head upright with the head top facing upwards to expose the skull of the head, cutting off the skull, observing exposed fish brain tissues under a microscope, then turning the fish brain tissues out from the position without cutting off the head to the front to expose sagitta in two spherical sacs, and firstly picking the sagitta and then picking the sagitta and the microotolith in the process of picking the otolith. The method provided by the invention can rapidly, efficiently and completely pick three pairs of otoliths of flounder juvenile fishes; in the process of picking the otoliths of the flounder larvae, the head is separated according to the body characteristics, the otoliths in the auditory kettle are picked firstly when the otoliths are picked, and then the greater-shaped sagitta otoliths and the greater-shaped microcristallites are picked, so that the loss of the smaller otoliths along with brain tissues in the picking process is avoided, and the time for picking three pairs of otoliths is shortened.
Preferably, in the step (1), the specific method for separating the head from the body of the flounder juvenile fish is as follows: firstly, the head of the flounder larva is cut backwards from the middle position of two eyes of the flounder larva along the gill cover rear edge from the anastomosis section, then the head of the flounder larva is cut off along the vertical vertebra of the gill cover rear edge from the head position, and the head is discarded to obtain the head.
According to the physiological structure of the flounder juvenile fish, the flounder juvenile fish is cut backwards from the mouth of the flounder juvenile fish along the middle position of two eyes to the rear edge of the gill cover, then the head of the flounder juvenile fish is cut off by the vertical vertebra without plugging the rear edge with salt from the head, and three pairs of complete otoliths are positioned in the head of the flounder juvenile fish. The method provided by the invention can be used for quickly and accurately shearing the head of the flounder juvenile fish, and the otoliths in the flounder juvenile fish are not damaged, so that the otolith picking time is shortened, and the otolith picking step is simplified.
Preferably, in the step (2), after the flounder larva is placed with its head facing upwards, the method further comprises removing muscle tissue above the skull of the fish head to expose the skull of the fish. According to the invention, after the fish head is cut off, the fish head comprises a part of tissue interfering with otolith extraction, and before the skull is treated, the muscle tissue on the skull is removed, so that the extraction efficiency is effectively improved.
Preferably, in the step (2), the specific method for cutting off the skull comprises the following steps: the skull of the fish head is cut open and then cut off.
In the method for rapidly picking the otoliths of the flounder larvae, the skull of the fish head is preferably cut to a depth of 0.8-1.2 mm.
Preferably, in the step (4), the brain tissue is firstly turned out from the rear cut part and then the sagittaria in the spherical sac is exposed.
Preferably, the method for rapidly picking otoliths of flounder larvae further comprises post-treatment of sagittaria, otoliths and otoliths in the step (4).
Preferably, the post-treatment comprises placing three pairs of otoliths in a surface dish, dripping deionized water, clamping the otoliths, then removing outer envelope and mucus of the otoliths, and drying. Further preferably, the otolith is held by a pointed forceps and the outer envelope and mucus of the otolith are removed by another pointed forceps. Further preferably, the drying is natural airing.
The invention has the beneficial effects that:
the method for quickly picking the otoliths of the flounder larvae, provided by the invention, is characterized in that the middle positions of two eyes of the scion section are cut backwards to the rear edge of a gill cover according to the structural characteristics of the flounder larvae, then the fish head is cut off from the top of the fish head in the direction vertical to the spine along the rear edge of the gill cover, and the head of the flounder larvae is separated from the body to obtain the head; then, the skull of the head of the fish is cut off to expose the brain tissue of the fish, the brain tissue is turned forward from the cut part at the back to expose the sagittaria pinnata in the spherical sac, the sagittaria pinnata is firstly picked out, and then the sagittaria pinnata and the micro-tragus pinnata are picked out. The method provided by the invention can quickly, accurately and completely pick out the three pairs of otoliths of the flounder juvenile fish, save the time for picking the otoliths of the flounder juvenile fish, shorten the picking period and have wide application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic microscopic view of three pairs of otoliths of juvenile flounder obtained in the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
The invention provides a method for quickly picking otoliths of flounder larvae, which specifically comprises the following steps:
(1) separating the head of flounder juvenile fish from the body to obtain the head of flounder juvenile fish; taking the juvenile flounder as an example, preferably, firstly, a scalpel is used for self-cutting the segment from the middle position of two eyes of the juvenile flounder to the rear edge of the gill cover, then the head of the juvenile flounder is not cut off from the head top position along the rear edge of the gill cover and perpendicular to the vertebra, and the body of the juvenile flounder is discarded to obtain the head of the juvenile flounder; after the treatment, the head of the juvenile flounder is quickly separated from the body, and three pairs of otoliths are completely retained in the head;
(2) placing the head of the juvenile flounder upright with the head facing upwards, and then cutting off the skull to obtain the treated head of the juvenile flounder; preferably, the head of the juvenile flounder cut in the step (1) is placed upright with the head top facing upwards, muscle tissue above the skull of the fish head is removed, the skull of the fish head is exposed, a scalpel is used for cutting the skull of the fish head by 0.8-1.2mm, and the skull is cut off at the depth;
(3) placing the treated head of the juvenile flounder under a microscope to expose the fish brain tissue; preferably, the head of the treated juvenile flounder is placed under a microscope, and the microscope is adjusted to clearly see the structure of the head of the juvenile flounder under a stereomicroscope;
(4) exposing sagittaria in the spherical sac, taking out the stellerite, and then taking out the sagittaria and the micro-otoliths to obtain three pairs of complete otoliths; preferably, under a stereoscopic microscope, a pair of pointed-end tweezers is respectively held by the left hand and the right hand, wherein the head of the tweezers is pressed, the fish brain tissue is turned out from the cut part at the rear part by the other pair of tweezers, the sagitta in the two spherical sacs can be exposed in the step, the problem that the tragus stones in the auditory kettle on the side wall are pulled out and lost by pulling the brain tissue from the two sides is avoided, then the tragus stones are picked, in the process of picking the tragus stones, two smaller tragus stones on the side wall are preferably picked, and then two pairs of larger tragus stones and micro tragus stones are picked by the tweezers; according to the step of picking otolith, the otolith is picked firstly, so that the loss of the otolith along with brain tissue is avoided, therefore, the otolith in the auditory canal kettle is found for the first time in the process of picking the otolith, and after the otolith is picked, the otolith with larger shape and micro otolith are picked, so that three pairs of otoliths of the juvenile flounder can be picked completely and quickly;
(5) and (3) post-treatment: and (4) carrying out post-treatment on the three pairs of otoliths taken out in the step (4), placing the three pairs of otoliths in a watch glass, dripping deionized water into the watch glass, clamping the otoliths by using a pointed forceps, removing an outer envelope and mucus of the otoliths by using another pointed forceps, and naturally drying to obtain clean and complete three pairs of otoliths.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (8)
1. A method for rapidly picking otoliths of flounder larvae is characterized by comprising the following steps:
(1) separating the head of flounder juvenile fish from the body to obtain the head of flounder juvenile fish;
(2) placing the head of the flounder juvenile fish upright, and then cutting off the skull to obtain the head of the treated flounder juvenile fish;
(3) placing the head of the treated flounder juvenile fish under a microscope to expose the brain tissue of the fish;
(4) exposing sagittaria in the spherical sac, taking out the stellerite, and then taking out the sagittaria and the micro-otoliths to obtain three pairs of complete otoliths;
the specific method for separating the head from the body of the flounder juvenile fish comprises the following steps: firstly, cutting the head part of the flounder juvenile fish from the head part of the flounder juvenile fish along the vertical vertebra of the rear edge of the gill cover from the middle position of the two eyes of the flounder juvenile fish of the kish flounder to the rear edge of the gill cover, and abandoning the fish body to obtain the head part;
after the head top of the flounder juvenile fish is placed upright, the method also comprises the step of rejecting muscle tissues above the head cranium of the fish to expose the head cranium of the fish.
2. The method for rapidly picking otolith of flounder larvae according to claim 1, wherein the specific method for cutting off the skull in the step (2) comprises the following steps: the skull of the fish head is cut open and then cut off.
3. The method for rapidly picking otolith of flounder larvae according to claim 1, wherein the skull of the fish head is cut to a depth of 0.8 to 1.2 mm.
4. The method for rapidly picking otolith of flounder larvae according to claim 1, wherein in the step (4), the brain tissue is first turned out from the posterior cut to expose the otolith vector in the sacculus.
5. The method for rapidly picking otolith of flounder larvae according to claim 1, wherein the step (4) further comprises post-treatment of sagittaria, microcolonia and otolith.
6. The method for rapidly picking otolith from flounder larvae according to claim 5, wherein the post-treatment comprises placing three pairs of otoliths in a petri dish, adding deionized water dropwise to the petri dish, holding the otoliths, removing outer envelope and mucus of the otoliths, and drying the otoliths.
7. The method for rapidly picking otolith of flounder larvae according to claim 6, wherein the otolith is held by a pointed forceps and the external envelope and mucus of the otolith are removed by another pointed forceps.
8. The method for rapidly picking otolith of flounder larvae according to claim 6, wherein the drying is natural air drying.
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| CN202011207239.8A CN112335716B (en) | 2020-11-03 | 2020-11-03 | Method for rapidly picking otoliths of flounder larvae |
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| CN202011207239.8A CN112335716B (en) | 2020-11-03 | 2020-11-03 | Method for rapidly picking otoliths of flounder larvae |
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| CN112335716B true CN112335716B (en) | 2022-04-15 |
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Citations (6)
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| CN202656203U (en) * | 2012-05-31 | 2013-01-09 | 中国水产科学研究院长江水产研究所 | Scissors for picking up miniature fish otolith |
| CN104198260A (en) * | 2014-09-15 | 2014-12-10 | 中国水产科学研究院黑龙江水产研究所 | Method for acquiring clean long-placed fish otolith |
| CN105028281A (en) * | 2015-08-17 | 2015-11-11 | 江苏省海洋水产研究所 | Method for rapidly picking sagitta from fusiform and compressiform fish |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
| CN105158035A (en) * | 2015-08-20 | 2015-12-16 | 江苏省海洋水产研究所 | Production method of little yellow croaker larval otolith sagittal plane sections for micro-chemical analysis |
| JP2019066442A (en) * | 2017-10-05 | 2019-04-25 | 国立大学法人東京海洋大学 | Primary embedding block for observing otolith, sample block for observing otolith, and method of preparing otolith sample piece |
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2020
- 2020-11-03 CN CN202011207239.8A patent/CN112335716B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202656203U (en) * | 2012-05-31 | 2013-01-09 | 中国水产科学研究院长江水产研究所 | Scissors for picking up miniature fish otolith |
| CN104198260A (en) * | 2014-09-15 | 2014-12-10 | 中国水产科学研究院黑龙江水产研究所 | Method for acquiring clean long-placed fish otolith |
| CN105028281A (en) * | 2015-08-17 | 2015-11-11 | 江苏省海洋水产研究所 | Method for rapidly picking sagitta from fusiform and compressiform fish |
| CN105158035A (en) * | 2015-08-20 | 2015-12-16 | 江苏省海洋水产研究所 | Production method of little yellow croaker larval otolith sagittal plane sections for micro-chemical analysis |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
| JP2019066442A (en) * | 2017-10-05 | 2019-04-25 | 国立大学法人東京海洋大学 | Primary embedding block for observing otolith, sample block for observing otolith, and method of preparing otolith sample piece |
Non-Patent Citations (2)
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| 一种新的鱼龄判别法;宋树恢;《世界农业》;19881110(第11期);全文 * |
| 叉尾斗鱼仔鱼耳石形态发育与日轮形成特征;赵天等;《中国水产科学》;20101115(第06期);全文 * |
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