CN112322483A - Microbial activity determination system and microbial activity determination method - Google Patents
Microbial activity determination system and microbial activity determination method Download PDFInfo
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- CN112322483A CN112322483A CN202011148556.7A CN202011148556A CN112322483A CN 112322483 A CN112322483 A CN 112322483A CN 202011148556 A CN202011148556 A CN 202011148556A CN 112322483 A CN112322483 A CN 112322483A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 61
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 61
- 239000001301 oxygen Substances 0.000 claims abstract description 61
- 238000006243 chemical reaction Methods 0.000 claims abstract description 48
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 18
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 57
- 229910002651 NO3 Inorganic materials 0.000 claims description 21
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 21
- 230000001651 autotrophic effect Effects 0.000 claims description 20
- 239000011159 matrix material Substances 0.000 claims description 18
- 238000005273 aeration Methods 0.000 claims description 15
- 230000012010 growth Effects 0.000 claims description 15
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 239000010865 sewage Substances 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 10
- 238000005259 measurement Methods 0.000 claims description 9
- 238000003556 assay Methods 0.000 claims description 8
- 229910021529 ammonia Inorganic materials 0.000 claims description 7
- 230000036284 oxygen consumption Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 230000009604 anaerobic growth Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 238000006396 nitration reaction Methods 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 239000010802 sludge Substances 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 239000005416 organic matter Substances 0.000 abstract description 2
- 125000001477 organic nitrogen group Chemical group 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/40—Detection of gases
- C12Q2304/44—Oxygen
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to a microbial activity measuring system and a microbial activity measuring method, wherein the system comprises: the device comprises a tester, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument and a dissolved oxygen DO measuring instrument; the measuring instrument is connected with the reaction tank through a plurality of sets of pipelines, one end of each pipeline is fixedly arranged on the measuring instrument, and the other end of each pipeline is positioned below the liquid level in the reaction tank; the multiple sets of pipelines are respectively provided with an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument and a dissolved oxygen DO measuring instrument; one or two of the multiple sets of pipelines are standby pipelines; one end of the pipeline provided with the air pump and positioned in the reaction tank extends to be close to the bottommost part of the reaction tank; the tester is connected with a system control cabinet. The invention can accurately control the concentration of dissolved oxygen in the reaction process; the biodegradability of the organic matter is rapidly measured; overcoming the influence of the degradation of organic nitrogen on the determination of the nitrification rate; the denitrification potential of the activated sludge is conveniently measured; the reactor temperature is controllable and is not affected by the ambient temperature.
Description
Technical Field
The invention relates to a microbial activity measuring system and a microbial activity measuring method, in particular to a microbial activity measuring system and a microbial activity measuring method, which can conveniently acquire relevant signals and set parameters and can acquire relevant parameters from an interface by an operator after a reaction period is finished.
Background
The microbial activity analyzer (ABAM) is a specialized instrument developed by engineers of the american biochemical science and technology company to measure mainly some of the most important reaction rates of activated sludge. The ABAM has two modules, an electronic control module and a reactor module, wherein the reactor module consists of two batch reactors, and each reactor 4L is provided with a temperature control device, a stirrer and an aeration device, so that anaerobic, anoxic, aerobic and other conditions can be simulated. Each reactor is provided with various electrodes, and indexes such as NH3-N/NO3-N/DO/pH/ORP, temperature and the like can be measured. All power and signal lines of the reactor module are connected to the electronic control module. The electronic control module is an ABAM control center and is provided with a notebook computer, and the ABAM is controlled to operate by software installed in the computer to acquire relevant operation data of the reactor.
The ABAM is a sequencing batch reactor with an intelligent control function and is provided with a stirrer, a temperature control device and an aeration device, and can simulate actual operation conditions such as anaerobic operation, anoxic operation, aerobic operation and the like. And according to the experimental purpose, the water inlet characteristics and the sludge activity condition of the sewage plant, such as the biodegradability of organic matters, nitrification and denitrification potentials, the biological removal capacity of phosphorus and other information are analyzed. Through measurement and by combining water inlet and outlet data, technological process control parameters are optimized, and the treatment requirement of stable standard discharge of a sewage plant is met.
Disclosure of Invention
In view of the above problems, the present invention provides a system and a method for measuring microbial activity, which can conveniently collect relevant signals and set parameters, and after a reaction cycle is completed, an operator can obtain relevant parameters from an interface.
The invention solves the technical problems through the following technical scheme: a microbial activity assay system; the microbial activity assay system comprises: a determinator, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument, a dissolved oxygen DO measuring instrument,
The measuring instrument is connected with the reaction tank through a plurality of sets of pipelines, one end of each pipeline is fixedly arranged on the measuring instrument, and the other end of each pipeline is positioned below the liquid level in the reaction tank;
the multiple sets of pipelines are respectively provided with an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument and a dissolved oxygen DO measuring instrument; one or two of the multiple sets of pipelines are standby pipelines;
one end of the pipeline provided with the air pump and positioned in the reaction tank extends to be close to the bottommost part of the reaction tank;
the tester is connected with a system control cabinet.
In a specific embodiment of the present invention: and a heating gasket is arranged below the reaction tank and is connected to the tester through an electric wire.
In a specific embodiment of the present invention: the heating gasket is provided with temperature control.
In a specific embodiment of the present invention: the thickness range of the heating gasket is as follows: 2-20 cm.
In a specific embodiment of the present invention: and a stirrer is arranged in the reaction tank.
In a specific embodiment of the present invention: the dissolved oxygen DO measuring instrument is a dissolved oxygen DO measuring instrument with a temperature electrode.
In a specific embodiment of the present invention: the reaction tank is a 3-8L reaction tank.
A method for measuring the microbial activity by using the system comprises the following steps:
step (1): installing a tester, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument, a dissolved oxygen DO measuring instrument, a plurality of sets of pipelines, a stirrer, a heating gasket and a system control cabinet;
step (2): oxygen consumption rate determination OUR;
and (3): the nitration rate is used for measuring NUR;
and (4): the rate of denitrification determines DNUR.
In a specific embodiment of the present invention: the oxygen consumption rate OUR determination in the step (2) specifically comprises the following steps:
(1) inputting a dissolved oxygen target value including a high value and a low value into a system control cabinet; starting an automatic aeration control program;
(2) when the maximum target value of the dissolved oxygen is not reached, starting aeration; and when the maximum target value of the dissolved oxygen is reached, stopping aeration:
(3) and when the dissolved oxygen in the sewage is reduced to the set minimum target value of the dissolved oxygen, starting aeration:
(4) and calculating the aerobic rate OUR through a formula (1) according to the time change by changing the concentration of the dissolved oxygen: the concrete formula is as follows:
wherein Y isH-heterotrophic bacteria productivity factor;
YA-autotrophyThe bacteria yield coefficient;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNH-mass concentration of dissolved ammonia nitrogen;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-heterotrophic bacteria concentration.
In a specific embodiment of the present invention: the method for measuring NUR by the nitrification rate in the step (3) specifically comprises the following steps:
(1) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(2) when the ammonia nitrogen concentration measurement value in the biological pond is stable, quantitatively adding a known ammonia nitrogen concentration reagent through a peristaltic pump, and when the ammonia nitrogen concentration measurement value is recovered to the reagent adding level, solving an ODEs equation through a formula (2) to calculate the nitrification rate, wherein the concrete formula is as follows:
wherein the content of the first and second substances,
is the nitrification rate;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNHsubstances of soluble ammonia nitrogenQuantitative concentration;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of the heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-a heterotrophic bacteria concentration;
in a specific embodiment of the present invention: the DNUR determination method based on the denitrification rate in the step (4) specifically comprises the following steps:
(1) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(2) when the measured value of the nitrate concentration in the biological pond is stable, quantitatively adding a known nitrate concentration reagent through a peristaltic pump, and when the measured value of the nitrate concentration is recovered to the added reagent level, calculating the nitrification rate by solving an ODEs equation according to a formula (3), wherein the specific formula is as follows:
wherein the content of the first and second substances,
is the nitrate conversion rate;
(3)
wherein the content of the first and second substances,
is the nitrate conversion rate;
μH-maximum specific growth rate of heterotrophic bacteria;
SNO3-mass concentration of soluble nitrate;
SOconcentration of dissolved oxygenDegree;
SCOD-fast biodegradable matrix concentration;
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,H-the oxygen half-saturation coefficient;
KNO3-the half-saturation factor of the nitrate,
XB,H-a heterotrophic bacteria concentration;
ηg-correction factor for anaerobic growth of heterotrophic bacteria.
The positive progress effects of the invention are as follows: the microbial activity tester provided by the invention has the following advantages: the concentration of dissolved oxygen in the reaction process can be accurately controlled; the biodegradability of the organic matter is rapidly measured; overcoming the influence of the degradation of organic nitrogen on the determination of the nitrification rate; the denitrification potential of the activated sludge is conveniently measured; the reactor temperature is controllable and is not affected by the ambient temperature.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
The following are the names corresponding to the reference numbers in the invention:
the device comprises a determinator 1, a reaction tank 2, an air pump 3, a peristaltic pump 4, an ammonia nitrogen measuring instrument 5, a nitrate nitrogen measuring instrument 6, a dissolved oxygen DO measuring instrument 7, a stirrer 8, a standby pipeline 9, a system control cabinet 10 and a heating gasket 11.
Detailed Description
The following provides a detailed description of the preferred embodiments of the present invention with reference to the accompanying drawings.
Fig. 1 is a schematic view of the overall structure of the present invention. As shown in FIG. 1, the present invention provides a system for measuring microbial activity; the microbial activity assay system includes: the system comprises a tester 1, a reaction tank 2, an air pump 3, a peristaltic pump 4, an ammonia nitrogen measuring instrument 5, a nitrate nitrogen measuring instrument 6 and a dissolved oxygen DO measuring instrument 7; the tester 1 is connected with the reaction tank 2 through a plurality of sets of pipelines, one end of each pipeline is fixedly installed on the tester 1, and the other end of each pipeline is located below the liquid level in the reaction tank.
The air pump 3, the peristaltic pump 4, the ammonia nitrogen measuring instrument 5, the nitrate nitrogen measuring instrument 6 and the dissolved oxygen DO measuring instrument 7 are respectively arranged on the plurality of sets of pipelines; one or two of the plurality of sets of pipes are spare pipes 9.
One end of the pipeline provided with the air pump and positioned in the reaction tank extends to be close to the bottommost part of the reaction tank. The measuring instrument 1 is connected with a system control cabinet 10.
A heating pad 11 is arranged below the reaction tank 2, and the heating pad 11 is connected to the tester 1 through an electric wire. The hot pad 14 is a heating pad provided with temperature control, and the thickness range of the heating pad is as follows: 2-20 cm.
A stirrer 8 is installed in the reaction tank 2. The dissolved oxygen DO measuring instrument is a dissolved oxygen DO measuring instrument with a temperature electrode. The reaction tank is 3-8L. In a specific implementation process, the above parameters may be selected according to specific requirements.
The invention provides a method for measuring microbial activity, which comprises the following steps:
step (1): installing a tester, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument, a dissolved oxygen DO measuring instrument, a plurality of sets of pipelines, a stirrer, a heating gasket and a system control cabinet;
step (2): oxygen consumption rate determination OUR;
and (3): the nitration rate is used for measuring NUR;
and (4): the rate of denitrification determines DNUR.
The oxygen consumption rate OUR determination in the step (2) specifically comprises the following steps:
(201) inputting a dissolved oxygen target value including a high value and a low value into a system control cabinet; starting an automatic aeration control program;
(202) when the maximum target value of the dissolved oxygen is not reached, starting aeration; and when the maximum target value of the dissolved oxygen is reached, stopping aeration:
(203) and when the dissolved oxygen in the sewage is reduced to the set minimum target value of the dissolved oxygen, starting aeration:
(204) and calculating the aerobic rate OUR through a formula (1) according to the time change by changing the concentration of the dissolved oxygen: the concrete formula is as follows:
wherein Y isH-heterotrophic bacteria productivity factor;
YA-an autotrophic bacteria productivity factor;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNH-mass concentration of dissolved ammonia nitrogen;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-heterotrophic bacteria concentration.
The method for measuring NUR by the nitrification rate in the step (3) specifically comprises the following steps:
(301) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(302) when the ammonia nitrogen concentration measurement value in the biological pond is stable, quantitatively adding a known ammonia nitrogen concentration reagent through a peristaltic pump, and when the ammonia nitrogen concentration measurement value is recovered to the reagent adding level, solving an ODEs equation through a formula (2) to calculate the nitrification rate, wherein the concrete formula is as follows:
wherein the content of the first and second substances,
is the nitrification rate;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNH-mass concentration of dissolved ammonia nitrogen;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of the heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-heterotrophic bacteria concentration.
The DNUR determination method based on the denitrification rate in the step (4) specifically comprises the following steps:
(401) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(402) when the measured value of the nitrate concentration in the biological pond is stable, quantitatively adding a known nitrate concentration reagent through a peristaltic pump, and when the measured value of the nitrate concentration is recovered to the added reagent level, calculating the nitrification rate by solving an ODEs equation according to a formula (3), wherein the specific formula is as follows:
wherein the content of the first and second substances,
is the nitrate conversion rate;
(3)
wherein the content of the first and second substances,
is the nitrate conversion rate;
μH-maximum specific growth rate of heterotrophic bacteria;
SNO3-mass concentration of soluble nitrate;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,H-the oxygen half-saturation coefficient;
KNO3-the half-saturation factor of the nitrate,
XB,H-a heterotrophic bacteria concentration;
ηg-correction factor for anaerobic growth of heterotrophic bacteria.
The invention is installed in a laboratory and provides a visual interface for users. Sampling by an operator and putting into a reaction tank. The invention collects relative signal and parameter, to realize automatic aeration and automatic drug feeding. After the reaction period is finished, the operator can obtain relevant parameters from the interface.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims and their equivalents.
Claims (11)
1. A microbial activity assay system; the method is characterized in that: the microbial activity assay system comprises: a determinator, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument, a dissolved oxygen DO measuring instrument,
The measuring instrument is connected with the reaction tank through a plurality of sets of pipelines, one end of each pipeline is fixedly arranged on the measuring instrument, and the other end of each pipeline is positioned below the liquid level in the reaction tank;
the multiple sets of pipelines are respectively provided with an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument and a dissolved oxygen DO measuring instrument; one or two of the multiple sets of pipelines are standby pipelines;
one end of the pipeline provided with the air pump and positioned in the reaction tank extends to be close to the bottommost part of the reaction tank;
the tester is connected with a system control cabinet.
2. The system according to claim 1, wherein a heating pad is installed below the reaction tank, and the heating pad is connected to the measuring instrument through a wire.
3. The microbial activity assay system of claim 2, wherein the heated pad is a heated pad provided with temperature control.
4. A microbiological activity assay system according to claim 2 or 3 wherein the thickness of said heated pad is in the range: 2-20 cm.
5. The system according to claim 1, wherein an agitator is installed in the reaction tank.
6. The microbial activity assay system of claim 1, wherein said dissolved oxygen DO meter is a dissolved oxygen DO meter with a temperature electrode.
7. The system according to claim 1, wherein the reaction tank is a 3-8L reaction tank.
8. A method for measuring a microbial activity, which comprises the steps of:
step (1): installing a tester, a reaction tank, an air pump, a peristaltic pump, an ammonia nitrogen measuring instrument, a nitrate nitrogen measuring instrument, a dissolved oxygen DO measuring instrument, a plurality of sets of pipelines, a stirrer, a heating gasket and a system control cabinet;
step (2): oxygen consumption rate determination OUR;
and (3): the nitration rate is used for measuring NUR;
and (4): the rate of denitrification determines DNUR.
9. The method for measuring microbial activity according to claim 8, wherein the oxygen consumption rate OUR measurement in step (2) comprises the steps of:
(1) inputting a dissolved oxygen target value including a high value and a low value into a system control cabinet; starting an automatic aeration control program;
(2) when the maximum target value of the dissolved oxygen is not reached, starting aeration; and when the maximum target value of the dissolved oxygen is reached, stopping aeration:
(3) and when the dissolved oxygen in the sewage is reduced to the set minimum target value of the dissolved oxygen, starting aeration:
(4) and calculating the aerobic rate OUR through a formula (1) according to the time change by changing the concentration of the dissolved oxygen: the concrete formula is as follows:
wherein Y isH-heterotrophic bacteria productivity factor;
YA-an autotrophic bacteria productivity factor;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNH-mass concentration of dissolved ammonia nitrogen;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-heterotrophic bacteria concentration.
10. The method for measuring microbial activity according to claim 1, wherein the measurement of NUR by the nitrification rate in the step (3) specifically comprises the steps of:
(1) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(2) when the ammonia nitrogen concentration measurement value in the biological pond is stable, quantitatively adding a known ammonia nitrogen concentration reagent through a peristaltic pump, and when the ammonia nitrogen concentration measurement value is recovered to the reagent adding level, solving an ODEs equation through a formula (2) to calculate the nitrification rate, wherein the concrete formula is as follows:
wherein the content of the first and second substances,
is the nitrification rate;
μA-the maximum specific growth rate of autotrophic bacteria;
μH-maximum specific growth rate of heterotrophic bacteria;
SNH-mass concentration of dissolved ammonia nitrogen;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KNH-the ammonia half-saturation factor of the autotrophic bacteria,
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,A-the oxygen half-saturation coefficient of autotrophic bacteria;
KO,H-the oxygen half-saturation coefficient of the heterotrophic bacteria;
XB,A-autotrophic bacteria concentration;
XB,H-a heterotrophic bacteria concentration;
11. the method for measuring microbial activity according to claim 1, wherein the DNUR determination of the denitrification rate in the step (4) comprises the following steps:
(1) collecting sewage mixed liquor of the biological tank, and stirring and operating for 3-5 hours;
(2) when the measured value of the nitrate concentration in the biological pond is stable, quantitatively adding a known nitrate concentration reagent through a peristaltic pump, and when the measured value of the nitrate concentration is recovered to the added reagent level, calculating the nitrification rate by solving an ODEs equation according to a formula (3), wherein the specific formula is as follows:
wherein the content of the first and second substances,
is the nitrate conversion rate;
(3)
wherein the content of the first and second substances,
is the nitrate conversion rate;
μH-maximum specific growth rate of heterotrophic bacteria;
SNO3-mass concentration of soluble nitrate;
SO-dissolved oxygen concentration;
SCOD-fast biodegradable matrix concentration;
KCOD-the half-saturation factor of the rapidly biodegradable matrix;
KO,H-the oxygen half-saturation coefficient;
KNO3-the half-saturation factor of the nitrate,
XB,H-a heterotrophic bacteria concentration;
ηg-correction factor for anaerobic growth of heterotrophic bacteria.
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