CN112315505A - Virus detection method for throat swab with isolation, inactivation and protection functions - Google Patents

Virus detection method for throat swab with isolation, inactivation and protection functions Download PDF

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Publication number
CN112315505A
CN112315505A CN202011130945.7A CN202011130945A CN112315505A CN 112315505 A CN112315505 A CN 112315505A CN 202011130945 A CN202011130945 A CN 202011130945A CN 112315505 A CN112315505 A CN 112315505A
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collection
swab
inactivation
detection
chamber
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CN202011130945.7A
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杨树林
杨帆
崔萌
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Shandong First Medical University and Shandong Academy of Medical Sciences
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Shandong First Medical University and Shandong Academy of Medical Sciences
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Publication of CN112315505A publication Critical patent/CN112315505A/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045Devices for taking samples of body liquids
    • A61B10/0051Devices for taking samples of body liquids for taking saliva or sputum samples

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pulmonology (AREA)
  • Pathology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a pharynx swab virus detection method with isolation inactivation protection, and relates to a virus detection method. The invention realizes the complete isolation of the collection personnel and the personnel to be detected, and achieves the all-round safety protection; the collection personnel no longer have the burden in mind when effective protection, ensure the collection personnel more high-efficient accurate collection of time more, combine the ring beam light source structure, accuracy and efficiency are also corresponding high, and the protective detection is gathered to the pharynx swab of the extremely dangerous infectious disease source of specially adapted.

Description

Virus detection method for throat swab with isolation, inactivation and protection functions
Technical Field
The invention relates to a virus detection method, in particular to a pharynx swab virus detection method with isolation, inactivation and protection, which is suitable for a pharynx swab detection and collection implementation process of high-risk infectious diseases such as new coronavirus.
Background
In view of the high-risk infection and hidden characteristics of the new coronavirus, detection aiming at the new coronavirus also becomes important in all countries in the world, however, throat swab detection of the new coronavirus belongs to high-risk operation aiming at medical staff, meanwhile, the risk of infection also exists aiming at staff participating in detection, a collection staff needs to contact with detected staff face to face, the new coronavirus is difficult to be directly detected, and therefore the problem of false negative rate is often caused by collection nurses in order to avoid the risk. If Guo Jing of university of capital medicine is obtained in comparison of nucleic acid detection of 31 novel coronavirus infection cases pharynx swab and sputum specimen virus, the novel coronavirus mainly attacks and infects lower respiratory tract cells, so that the collection of the lower respiratory tract specimen has more advantages on virus detection, the detection rate of the pharynx swab is low, and the pharynx swab is possibly related to different virus contents of nurses in sampling manipulation and sampling positions close to the outer side of pharynx or in courses of patients.
Patent number 202010504862.3 south university Xiangya two hospitals bear force introduced a portable nasopharynx swab sampling device, including operating handle, transparent baffle, intubate and sampling head, operating handle be 7 style of calligraphy settings to at the anterior mounting of this undergauge installation department have the module of making a video recording and illumination module, although this patent has realized portable installation and detection, this protection mode protection scope is limited.
Patent No. 202010126565. X populus teaches a closed pharyngeal swab sampling device. The mouth and nose parts of the patient are covered properly in a closed manner, so that the extremely high risk that medical staff directly face respiratory tract pathogen infection of the patient when collecting throat swab samples of the patient is avoided; the oral cavity real-time image is displayed outside the body by leading in a camera with a light source in the oral cavity, and medical staff can correctly and quickly acquire an ideal throat swab specimen by real-time image guidance. Patent No. 202010156523.0, shanghai chang zheng hospital li ling etc. introduced a pharynx swab collection assembly, and this subassembly adopts the collection face guard to reduce the possibility that medical personnel contacted the droplet, reduces the infection risk, also can illuminate patient's oral cavity pharynx simultaneously, thereby has solved medical personnel and has seen the problem that unclear collection position can't accurately gather the sample and lead to false negative rate height.
Patent No. 202010146057.8, Ningxia medical university general hospital week yoga introduced a protection device is gathered to pharynx swab, and the device adopts the vertical division board of arranging, and the division board adopts transparent material to make, and one side of division board is for observing the side, and the other end side of division board is the inspection side, and the tongue depressor, components such as layer board are arranged and are had disposable protection film.
Although the isolation is realized to a certain degree, absolute isolation environments of detection personnel and detected people are not realized, so that complete isolation between collection personnel and the detection personnel cannot be realized, and corresponding subsequent virus attaching and adsorbing inactivation measures are not provided.
Disclosure of Invention
The invention aims to provide a method for detecting virus by using a pharyngeal swab with isolation and inactivation protection, which designs a brief detection flow and provides necessary parameters and equipment, so that a detector for detecting the virus is completely isolated from a detected person, the adsorption and inactivation treatment of the virus is ensured, the detection accuracy is improved, and the detection efficiency is accelerated.
The purpose of the invention is realized by the following technical scheme:
a method for detecting virus by using a pharyngeal swab with isolation and inactivation protection comprises the following steps of collecting, transporting and inactivating:
1) throat swab collection
(1) Detecting that a worker waits for operation outside a window of the collection room;
(2) the personnel to be tested enters an idle collection room;
(3) a detection person takes down the collection chamber through the protective gloves, hangs the collection chamber, and installs a throat swab collection gun;
(4) a detection person holds a hand to open a power supply of the collecting gun for detection, and operates the tongue depressor with the other hand to perform throat swab detection;
(5) under the protection of absolute safety protection, the detection personnel can carry out various complex sampling operations safely;
(6) after collection, a detection person pinches off the throat swab and places the test sample in the throat swab sample;
the person to be detected leaves the collection chamber, and the detection chamber door is closed;
2) transport process control
(1) A detection person operates a button, and the throat swab fixing seat is positioned at a sample transportation inlet by the belt conveyor through the photoelectric sensor;
(2) a detection person opens the sample transport inlet cover and places the throat swab sample on the throat swab fixing seat;
(3) starting a transportation button, and stably transporting to a collection chamber at the speed of 0.2 m/s;
(4) the staff in the collection room hears the alarm to take out the throat swab sample due to operation;
placing the pharynx swab sample in a pharynx swab freezing and storing box by a collection room worker;
3) inactivation process control
(1) During the transport process of the throat swab sample, a detector starts an inactivation button;
(2) the operating radius of the ultraviolet inactivation lamp is 2 meters, the inactivation time is started according to the characteristics of the detected pathogen, and the time is controllable;
(3) after inactivation is finished, the detection personnel hear the prompt tone to operate and open the air valve of the detection chamber;
(4) blowing the detection chamber by 0.4Mpa compressed air for 5min, and opening the collection chamber door;
(5) the next person to be tested enters the collection room;
4) sampling control
(1) When collecting a certain amount of throat swabs or after a certain time interval, workers in the collecting chamber carry out inactivation operation on the collecting chamber and operation in the collecting chamber;
(2) collecting chamber staff start purging the detecting chamber for 5min by using 0.4Mpa compressed air for the collecting chamber and then open a collecting door;
(3) the pharyngeal swab sample was removed for assay and the collection chamber door was closed.
According to the method for detecting the virus by using the pharyngeal swab with the isolation and inactivation protection, the collection is required to be provided with arrays according to the scale of a collection mechanism, and a collection chamber is required to be a sealing door and is provided with an observation window.
In the transport process, after being collected by a sample detector, the sample detector quickly passes through the transport inlet and the inlet sealing cover and is positioned and conveyed to the throat swab freezing and storing box of the collecting chamber through the servo belt system.
The pharynx swab virus detection method with the isolation and inactivation protection function comprises a servo speed reduction motor, a pharynx swab fixing seat and a carrier roller group.
According to the pharynx swab virus detection method with isolation, inactivation and protection, the collection chamber is used for purging the detection chamber by adopting compressed air, the air source and air path structure of the collection chamber is air of an air compressor and is arranged in two paths, one path of servo belt system is arranged in each collection chamber and each collection chamber, and the other path of air source and air path structure is arranged in each collection chamber and each collection chamber.
The pharynx swab detection virus method with isolation and inactivation protection, the collection gun metal part is made of copper materials, the collection gun end is provided with the locking nut to fix the pharynx swab, the direct injection annular illuminating lamp is adopted, and the middle hole avoids shielding, so that the pharynx swab operation position is irradiated in a concentrated mode.
The virus detection method for the pharyngeal swab with the isolation and inactivation protection comprises a collection chamber, a servo belt system, protective gloves, an air path structure, a collection gun, an ultraviolet inactivation lamp, a pharyngeal swab sample and a pharyngeal swab freezing and storing box.
The invention has the advantages and effects that:
the invention realizes the complete isolation of the collection staff and the patients, simultaneously avoids the cross infection among the patients, stops the further propagation of new coronavirus diseases and achieves the all-round safety protection; the collection personnel can not bear in mind any more while effectively protecting, the collection personnel can be guaranteed to collect more efficiently and accurately for a longer time, and the accuracy and the efficiency are improved.
The method has the following specific technical effects:
the complete isolation is formed between the detected persons by dividing each collection chamber;
the detector adopts the glove box operation to realize complete isolation from the detected person;
the detection facility metal part adopts a copper structure to reduce the virus adsorption survival time;
after the detection is finished, an ultraviolet inactivation lamp is used for carrying out adsorption inactivation treatment operation on the acquisition room;
the inspector and the inspector realize face-to-face operation, and the inspector can be relieved to operate the inspector carefully because the inspector and the inspector are completely isolated; a detector operates the annular detection light source collecting gun by one hand and operates the tongue depressor by the other hand, so that the light source is orthographically acted on the detection part of the detector, and the detection accuracy is greatly improved;
after inactivation is finished, the detection chamber is treated by a compressed air source, so that the detection chamber can be put into use again after 5min of inactivation;
when the detection is finished, the detection personnel immediately convey the throat swab sample to the storage chamber through the belt, so that the working efficiency is improved, and the sample is rapidly stored;
the independent detection room of array has realized detecting simultaneously of a large amount of detection personnel, and forms absolute isolation space between each other, waits to detect personnel and has also realized absolute isolation around with the detection room, and the person that detects has also realized absolute isolation with being detected personnel.
Drawings
FIG. 1 is a schematic three-dimensional structure of an isolation inactivation protection device according to the present invention;
FIG. 2 is a front view of the isolation and deactivation prevention device of the present invention;
FIG. 3 is a top view of an isolation and inactivation protection device A-A of the present invention;
FIG. 4 is a side view of an isolation and deactivation guard of the present invention B- -B;
FIG. 5 is a front view of a servo belt system;
FIG. 6 is a top view of the servo belt system;
FIG. 7 is a left side view of the servo belt system configuration;
FIG. 8 is a front view of the collection gun configuration of the present invention;
fig. 9 is a top view of the collection gun configuration of the present invention.
Reference numbers in the figures: collection chamber 1, collection chamber 2, servo belt system 3, protective gloves 4, gas circuit structure 5, gather rifle 6, ultraviolet deactivation lamp 7, pharynx swab sample 8, the freezing case of keeping 9 of pharynx swab, sealing door 10, observation window 11, transport inlet 12, the sealed lid of entry 13, servo gear motor 14, pharynx swab fixing base 15, bearing roller group 16, pharynx swab rupture 17, pharynx swab 18, lock nut 19, direct injection annular light 20, light fixing base 21, handle 22, fixed hole site 23.
Detailed Description
The present specification will be described in further detail below by way of examples with reference to the accompanying drawings. The following examples are only one example of the present invention and do not represent the scope of the claims.
Throat swab collection
Detecting that a worker waits for operation outside a window of the collection room;
the personnel to be tested enters an idle collection room;
a detection person takes down the throat swab collecting gun hung in the collecting chamber (6) through the protective gloves and installs the throat swab;
a detection person holds a hand to open a power supply of the collecting gun for detection, and operates the tongue depressor with the other hand to perform throat swab detection;
under the protection of absolute safety protection, the detection personnel can carry out various complex sampling operations safely;
after collection, a detection person pinches off the throat swab and places the test sample in the throat swab sample;
the person to be detected leaves the collection chamber, and the detection chamber door is closed;
transport process control
A detection person operates a button, and the throat swab fixing seat is positioned at a sample transportation inlet by the belt conveyor through the photoelectric sensor;
the detection personnel open the sample transport inlet cover and place the throat swab sample on the throat swab fixing seat.
Starting a transportation button, and stably transporting to a collection chamber at the speed of 0.2 m/s;
the staff in the collection room hears the alarm to take out the throat swab sample due to operation;
collection room staff placed the pharyngeal swab samples in the pharyngeal swab cryopreservation box.
Inactivation process control
During the transport process of the throat swab sample, a detector starts an inactivation button;
the operating radius of the ultraviolet inactivation lamp is 2 meters, the inactivation time is started according to the characteristics of the detected pathogen, and the time is controllable;
after inactivation is finished, the detection personnel hear the prompt tone to operate and open the air valve of the detection chamber;
blowing the detection chamber by 0.4Mpa compressed air for 5min, and opening the collection chamber door;
the next person to be tested enters the collection room;
sampling control
When collecting a certain amount of throat swabs or after a certain time interval, workers in the collecting chamber carry out inactivation operation on the collecting chamber and operation in the collecting chamber;
collecting chamber staff start purging the detecting chamber for 5min by using 0.4Mpa compressed air for the collecting chamber and then open a collecting door;
the pharyngeal swab sample was removed for assay and the collection chamber door was closed.
The collection chamber 1 can be seen and collection mechanism scale sets up the array, and every collection chamber of group comprises taking sealing door 10 specially, collection chamber scale is length 1~2m, and the width is 1~2m, highly is 2~4m, and every collection chamber observation window 11 is transparent quartz glass, disposes protective gloves 4 and organizes, and protective gloves are vacuum glove box gloves commonly used, and the interval is 0.5~0.8m, highly is 1~1.5m and realizes gathering personnel's portable operation. The sample testing personnel rapidly pass through the transportation inlet 12 and the inlet sealing cover 13 after collection and are positioned and conveyed to the collection chamber 2 through the servo belt system 3. The system is provided with a pharynx swab freezing storage box 9, and a collector uses the protective gloves 1-2 groups to store pharynx swab samples 8 collected in the collection room. The servo belt system 3 consists of a servo speed reducing motor 14, a throat swab fixing seat 15 and a carrier roller group 16; the servo belt conveying is low-speed transmission, the transmission speed is 0.05-0.3 m/s, and stable transmission and accurate positioning of swab samples can be realized; a bracket and a position sensor are arranged at a fixed position of each acquisition room, when an acquisition worker finishes acquisition, an operation button is pressed, and the belt conveyor stops the fixed bracket at a specified position; the belt conveyor is also provided with a photoelectric sensor for detecting whether a sample exists in the collection chamber, and when the sample exists at the collection position of the collection chamber, the belt conveyor immediately stops transmission and reminds a collector to carry out related operation. The air source air path structure 5 comprises air of an air compressor, is arranged in two paths, a path of servo belt system and each collection chamber, and is used for continuously keeping the pressure of 0.12Mpa in the collection chambers; the other path of pressure is 0.3-0.5 Mpa, the air outlets are arranged in each collection chamber, 1-2 collection chambers are arranged in each collection chamber, the ultraviolet inactivation lamp 7 starts to operate after the collection of the throat swab is finished, the operation time is started according to the characteristics of the detected pathogen, and the time is controllable; and after the operation is finished, the valve is quickly opened to blow compressed air of 0.3-0.5 Mpa for 5min, so that the acquisition chamber can be immediately recovered to be used after the air blowing. Gather rifle 6's metal parts and adopt copper material entirely, including throat swab rupture 17, throat swab 18, lock nut 19, direct projection annular lighting lamp 20, light fixing base 21, handle 22, installation fixed hole site 23, gather rifle tip and adopt lock nut 19, fixed throat swab adopts direct projection annular lighting lamp 20, and the light diameter is 30~40mm, and middle part hole design can avoid sheltering from to realize concentrating shining throat swab operation position. The equipment and tools used by the invention comprise a collection chamber 1, a collection chamber 2, a servo belt system 3, protective gloves 4, an air channel structure 5, a collection gun 6, an ultraviolet inactivation lamp 7, a throat swab sample 8, a throat swab freezing storage box 9, a sealing door 10, an observation window 11, a transportation inlet 12, an inlet sealing cover 13, a servo speed-reducing motor 14, a throat swab fixing seat 15, a carrier roller group 16, a throat swab breaking part 17, a throat swab 18, a locking nut 19, a direct injection annular illuminating lamp 20, an illuminating lamp fixing seat 21, a handle 22 and a fixing hole site 23.
The invention is particularly suitable for the pharyngeal swab collection protective detection of extremely dangerous infectious disease sources.

Claims (7)

1. A method for detecting virus by using pharyngeal swab with isolation and inactivation protection is characterized by comprising the following steps of collecting, transporting and inactivating:
the implementation process comprises four operation steps of collection, transportation, inactivation and sampling;
1) throat swab collection
(1) Detecting that a worker waits for operation outside a window of the collection room;
(2) the personnel to be tested enters an idle collection room;
(3) a detection person takes down the collection chamber through the protective gloves, hangs the collection chamber, and installs a throat swab collection gun;
(4) a detection person holds a hand to open a power supply of the collecting gun for detection, and operates the tongue depressor with the other hand to perform throat swab detection;
(5) under the protection of absolute safety protection, the detection personnel can carry out various complex sampling operations safely;
(6) after collection, a detection person pinches off the throat swab and places the test sample in the throat swab sample;
the person to be detected leaves the collection chamber, and the detection chamber door is closed;
2) transport process control
(1) A detection person operates a button, and the throat swab fixing seat is positioned at a sample transportation inlet by the belt conveyor through the photoelectric sensor;
(2) a detection person opens the sample transport inlet cover and places the throat swab sample on the throat swab fixing seat;
(3) starting a transportation button, and stably transporting to a collection chamber at the speed of 0.2 m/s;
(4) the staff in the collection room hears the alarm to take out the throat swab sample due to operation;
placing the pharynx swab sample in a pharynx swab freezing and storing box by a collection room worker;
3) inactivation process control
(1) During the transport process of the throat swab sample, a detector starts an inactivation button;
(2) the operating radius of the ultraviolet inactivation lamp is 2 meters, the inactivation time is started according to the characteristics of the detected pathogen, and the time is controllable;
(3) after inactivation is finished, the detection personnel hear the prompt tone to operate and open the air valve of the detection chamber;
(4) blowing the detection chamber by 0.4Mpa compressed air for 5min, and opening the collection chamber door;
(5) the next person to be tested enters the collection room;
4) sampling control
(1) When collecting a certain amount of throat swabs or after a certain time interval, workers in the collecting chamber carry out inactivation operation on the collecting chamber and operation in the collecting chamber;
(2) collecting chamber staff start purging the detecting chamber for 5min by using 0.4Mpa compressed air for the collecting chamber and then open a collecting door;
(3) the pharyngeal swab sample was removed for assay and the collection chamber door was closed.
2. A method for detecting virus with isolation and inactivation protection for pharyngeal swab as claimed in claim 1, wherein the collection should be arranged in array according to the scale of the collection mechanism, the collection chamber must be a sealing door (10), and a viewing window (11) is provided.
3. A pharyngeal swab virus detection method with isolation and inactivation protection function according to claim 1, characterized in that during the transportation process, after the sample detection personnel collect the samples, the samples are rapidly transported to the collection chamber (2) through the transportation inlet (12) and the inlet sealing cover (13) and then are positioned and transported to the pharyngeal swab freezing and storing box (9) through the servo belt system (3).
4. A pharyngeal swab virus detection method with isolation and inactivation protection function according to claim 3, characterized in that the servo belt system (3) comprises a servo speed reduction motor (14), a pharyngeal swab fixing seat (15) and a carrier roller set (16).
5. The method for detecting virus by using pharyngeal swab with isolation, inactivation and protection as claimed in claim 1, wherein the collection chamber is purged by using compressed air, the air source and air path structure (5) is air compressor and is disposed in two paths, one path of servo belt system and each collection chamber and collection chamber, and the other path is disposed at the air outlet of each collection chamber and collection chamber.
6. The pharynx swab virus detection method with isolation and inactivation protection as claimed in claim 1, wherein the collection gun (6) is made of copper metal parts, the pharynx swab is fixed at the collection gun end through a locking nut (19), a direct-injection ring-shaped illuminating lamp (20) is adopted, and a middle hole avoids shielding, so that concentrated irradiation on a pharynx swab operation position is realized.
7. A pharynx swab virus detection method with isolation and inactivation protection function according to claim 1, characterized in that the method uses a device comprising a collection chamber (1), a collection chamber (2), a servo belt system (3), protective gloves (4), an air channel structure (5), a collection gun (6), an ultraviolet inactivation lamp (7), a pharynx swab sample (8) and a pharynx swab cryopreservation box (9).
CN202011130945.7A 2020-10-21 2020-10-21 Virus detection method for throat swab with isolation, inactivation and protection functions Withdrawn CN112315505A (en)

Priority Applications (1)

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CN202011130945.7A CN112315505A (en) 2020-10-21 2020-10-21 Virus detection method for throat swab with isolation, inactivation and protection functions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011130945.7A CN112315505A (en) 2020-10-21 2020-10-21 Virus detection method for throat swab with isolation, inactivation and protection functions

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202021105477U1 (en) 2020-04-21 2021-10-21 Stefanie Merse Medical technology holding device and holding device kit each for holding general medical, surgical or diagnostic devices or instruments and their use, in particular for data acquisition

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202021105477U1 (en) 2020-04-21 2021-10-21 Stefanie Merse Medical technology holding device and holding device kit each for holding general medical, surgical or diagnostic devices or instruments and their use, in particular for data acquisition
EP3900639A1 (en) * 2020-04-21 2021-10-27 Stefanie Merse Medical holding device for holding general medical, surgical or diagnostic devices or instruments and their use, in particular for data acquisition
DE102021126231A1 (en) 2020-04-21 2022-10-20 Stefanie Merse Medical technology holding device and holding device kit, each for holding general medical, surgical or diagnostic devices and their use in particular for data acquisition and manufacturing processes

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