CN112314797A - Feed additive for ruminants and preparation method and application thereof - Google Patents
Feed additive for ruminants and preparation method and application thereof Download PDFInfo
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- CN112314797A CN112314797A CN202011319076.2A CN202011319076A CN112314797A CN 112314797 A CN112314797 A CN 112314797A CN 202011319076 A CN202011319076 A CN 202011319076A CN 112314797 A CN112314797 A CN 112314797A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 60
- 241000282849 Ruminantia Species 0.000 title claims abstract description 34
- 239000003674 animal food additive Substances 0.000 title claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 34
- 229940088598 enzyme Drugs 0.000 claims abstract description 34
- 230000000813 microbial effect Effects 0.000 claims abstract description 27
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 19
- 239000011707 mineral Substances 0.000 claims abstract description 19
- 235000010755 mineral Nutrition 0.000 claims abstract description 19
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 17
- 229920001661 Chitosan Polymers 0.000 claims abstract description 13
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 13
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 13
- 239000010457 zeolite Substances 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 12
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 11
- 229940106157 cellulase Drugs 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 11
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- 239000000654 additive Substances 0.000 claims abstract description 9
- 230000000996 additive effect Effects 0.000 claims abstract description 9
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 8
- 239000003085 diluting agent Substances 0.000 claims abstract description 7
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 6
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 5
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 5
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 5
- 235000007715 potassium iodide Nutrition 0.000 claims abstract 2
- 238000002156 mixing Methods 0.000 claims description 20
- 241000235342 Saccharomycetes Species 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 11
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 241001494479 Pecora Species 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000015278 beef Nutrition 0.000 description 4
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920001221 xylan Polymers 0.000 description 3
- 150000004823 xylans Chemical class 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of feed additives, in particular to a feed additive for ruminants and a preparation method and application thereof. The additive comprises a complex enzyme preparation, a microbial preparation, a complex mineral substance and a diluent; the enzyme activity of the compound enzyme preparation comprises the following components: 800-1600U/g cellulase, 3500-4500U/g xylanase and 1000-2000U/g pectinase; the microbial preparation comprises the following components: bacillus subtilis at least 5 x 109CFU/g, the bacillus licheniformis is more than or equal to 5*109CFU/g, yeast is more than or equal to 2 x 108CFU/g; the compound mineral substance is a mixture of potassium iodide, ferrous sulfate and calcium phosphate; the diluent is chitosan and zeolite powder. The additive improves the energy conversion rate and the growth rate, can improve the immunity of the ruminant organism, does not contain any chemical additive, and is green and environment-friendly.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a feed additive for ruminants and a preparation method and application thereof.
Background
The feed additive is a small amount or trace substance added in the processes of feed production, processing and use, but has obvious effect and is used for improving the production performance of animals and ensuring the health of the animals.
In order to improve the production performance of ruminants, antibiotics and other essential substances are added into the cultured daily ration at present, but with the increasing use amount of antibiotics, the problems of drug resistance, residue, environmental pollution, product residue in livestock and poultry bodies and the like are more and more prominent, particularly the problem of drug resistance, so that the development of a safe, efficient and pollution-free feed additive for improving the growth performance of the ruminants, ensuring the health of the ruminants, saving the cost and the like has important significance.
Disclosure of Invention
Aiming at the problems existing in the breeding process of the ruminants in the prior art and the hazards brought by common medicines in the prior art, the invention provides an additive which is formed by mutually matching a complex enzyme preparation and a microbial preparation and is used for improving the growth performance of the ruminants and improving the immunity of the ruminants. The invention provides a feed additive for ruminants, which comprises a complex enzyme preparation, a microbial preparation, a complex mineral substance and a diluent;
the enzyme activity of the compound enzyme preparation comprises the following components: 800-1600U/g cellulase, 3500-4500U/g xylanase and 1000-2000U/g pectinase;
the microbial preparation comprises the following components: bacillus subtilis at least 5 x 109CFU/g, bacillus licheniformis is more than or equal to 5 x 109CFU/g, yeast is more than or equal to 2 x 108CFU/g；
The compound mineral substance is a mixture of potassium iodide, ferrous sulfate and calcium carbonate;
the diluent is chitosan and zeolite powder.
Preferably, the enzyme activity of the complex enzyme preparation comprises the following components: the cellulase is 1000-1500U/g, the xylanase is 3600-4200U/g, and the pectinase is 1200-1800U/g.
Preferably, the additive comprises the following raw materials in parts by weight:
15-30 parts of complex enzyme preparation, 10-30 parts of microbial preparation, 5-15 parts of complex mineral, 20-30 parts of chitosan and 10-20 parts of zeolite powder.
Preferably, the microbial preparation is bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and saccharomycetes and a culture thereof; the preparation method of the microbial preparation comprises the following steps:
s1, respectively carrying out activation culture on the bacillus subtilis strain, the bacillus licheniformis strain and the saccharomycete strain, and then carrying out amplification culture to obtain corresponding strains and culture solutions thereof;
s2, concentrating the strain obtained in the step S1 and a culture solution thereof, and freeze-drying to obtain bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and saccharomycetes and a culture thereof;
s3, mixing each strain obtained in S2 and the culture thereof to obtain the microbial preparation.
The invention also provides a preparation method of the feed additive for the ruminants, which comprises the following steps:
uniformly mixing cellulase, xylanase and pectinase to obtain a complex enzyme preparation;
respectively carrying out activation amplification culture on bacillus subtilis, bacillus licheniformis and saccharomycete, concentrating, freeze-drying corresponding culture solutions to obtain bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and saccharomycete and a culture thereof, and mixing to obtain a microbial preparation;
and fully mixing the compound mineral, the chitosan, the zeolite powder, the compound enzyme preparation, the microbial preparation and the compound mineral to obtain the feed additive for the ruminant.
The invention also provides application of the feed additive for the ruminants, wherein the addition amount of the feed additive for the ruminants in each ton of feed is 300-500 g.
Has the advantages that:
the feed additive for the ruminants disclosed by the invention adopts the mutual matching of the complex enzyme preparation and the microbial preparation, so that on one hand, the degradation of non-starch substances in the raw materials can be improved, the raw materials are easy to digest and absorb by the ruminants, the energy conversion rate is improved, the growth rate is improved, and meanwhile, the immunity of the organism of the ruminants can be improved, and the feed additive does not contain any chemical additive and is green and environment-friendly.
The composite mineral substance is added, so that the biological activity of the composite enzyme can be promoted, the promotion effect of the additive is improved, and the stability of the composite enzyme preparation can be ensured by adding the chitosan and the zeolite powder as diluents.
Detailed Description
In order to clearly illustrate the technical content of the present invention, the detailed description is given in conjunction with specific examples, and it is obvious that the examples are only the preferred embodiments of the technical solution, and other technical solutions which can be obviously derived by those skilled in the art from the technical content disclosed still belong to the protection scope of the present invention.
The enzyme adopted by the invention is related enzyme of Hunan Lierkang biological corporation, wherein the enzyme activities of the enzymes are respectively as follows: 30000U/g xylanase, 20000U/g cellulase and 30000U/g pectinase.
The activation medium adopted by the invention is as follows: 5g of beef extract, 8g of sodium chloride, 15g of peptone, 3g of glucose, 4g of ammonium sulfate, 8g of potassium hydrogen phosphate, 2g of calcium chloride, agar and 1000ml of distilled water, wherein the pH value is 7.0-7.2, and the beef extract is obtained by sterilization treatment.
The amplification culture solution adopted by the invention is as follows: glucose 1.2%, peptone 0.5%, beef extract 0.6%, dipotassium hydrogen phosphate 1.2%, trehalose 2%, calcium sulfate 0.1%, magnesium chloride 0.2%, sodium citrate 0.2%, and distilled water in balance, wherein the pH value is 7.0-7.2, and the beef extract is obtained through sterilization treatment.
Example 1
And uniformly mixing 4 parts of cellulase, 15 parts of xylan and 4 parts of pectinase to obtain the complex enzyme preparation.
Respectively placing Bacillus subtilis strain, Bacillus licheniformis strain and yeast strain in activation culture medium for activation culture, inoculating to amplification culture medium for amplification culture to obtain strain with concentration of 2 x 108CFU/ml, concentrating, freeze drying to obtain Bacillus subtilis and its culture mediumThe thallus concentration of the nutrient, the Bacillus licheniformis and the culture thereof, the yeast and the culture thereof is more than or equal to 2 x 108CFU/g, mixing 10 parts of bacillus subtilis and a culture thereof, 5 parts of bacillus licheniformis and a culture thereof, and 2 parts of saccharomycetes and a culture thereof to obtain a microbial preparation;
mixing 4 parts of potassium iodide, 4 parts of ferrous sulfate and 6 parts of calcium phosphate to obtain a composite mineral substance, mixing 26 parts of chitosan and 20 parts of zeolite powder, and uniformly mixing the composite enzyme preparation, the microbial preparation, the composite mineral substance, the chitosan and the zeolite powder to obtain the feed additive for the ruminant.
Example 2
And uniformly mixing 6 parts of cellulase, 13 parts of xylan and 6 parts of pectinase to obtain the complex enzyme preparation.
Respectively placing Bacillus subtilis strain, Bacillus licheniformis strain and yeast strain in activation culture medium for activation culture, inoculating to amplification culture medium for amplification culture to obtain strain with concentration of 2 x 108CFU/ml, concentrating, freeze drying to obtain Bacillus subtilis and its culture, Bacillus licheniformis and its culture, and yeast and its culture, with thallus concentration of 2 x 10 or more8CFU/g, mixing 6 parts of bacillus subtilis and a culture thereof, 12 parts of bacillus licheniformis and a culture thereof, and 2 parts of saccharomycetes and a culture thereof to obtain a microbial preparation;
5 parts of potassium iodide, 5 parts of ferrous sulfate and 5 parts of calcium phosphate are mixed to obtain a composite mineral substance, 25 parts of chitosan and 15 parts of zeolite powder are mixed uniformly to obtain the feed additive for the ruminant.
Example 3
And uniformly mixing 8 parts of cellulase, 12 parts of xylan and 6 parts of pectinase to obtain the complex enzyme preparation.
Respectively placing Bacillus subtilis strain, Bacillus licheniformis strain and yeast strain in activation culture medium for activation culture, inoculating to amplification culture medium for amplification culture to obtain strain with concentration of 2 x 108CFU/ml, concentrating and freeze drying to obtain Bacillus subtilisIts culture, Bacillus licheniformis and its culture, and yeast and its culture, and its thallus concentration is greater than or equal to 2 x 108CFU/g, mixing 10 parts of bacillus subtilis and a culture thereof, 10 parts of bacillus licheniformis and a culture thereof, and 4 parts of saccharomycetes and a culture thereof to obtain a microbial preparation;
mixing 6 parts of potassium iodide, 4 parts of ferrous sulfate and 6 parts of calcium phosphate to obtain a composite mineral substance, mixing 20 parts of chitosan and 16 parts of zeolite powder, and uniformly mixing the composite enzyme preparation, the microbial preparation, the composite mineral substance, the chitosan and the zeolite powder to obtain the feed additive for the ruminant.
Comparative example 1
The complex enzyme preparation in the embodiment 2 is omitted, and the other raw material compositions and the preparation method are completely the same as the embodiment 2.
Comparative example 2
The microbial preparation in example 2 was omitted, and the other raw material composition and the preparation method were exactly the same as in example 2.
Comparative example 3
The complex mineral in example 2 was omitted and the original composition and preparation method were exactly the same as in example 2.
Application example
The method comprises the steps of selecting fattening sheep with the age of 4 months and the weight of 20.25 +/-1.08, adding the feed additive for the ruminants obtained in examples 1-3 and comparative examples 1-2 into common feed in the embodiment, adding 400g of the feed per ton, uniformly stirring, applying the feed additive to the fattening sheep, feeding the fattening sheep for 3 months, measuring the influence of the feed additive on the production performance of the fattening sheep, and taking the common feed as a control group, wherein 5 fattening sheep are parallelly arranged in each group. The measurement results are shown in table 1.
TABLE 1 results of the influence of feed additives for ruminants on the production performance of fattening sheep
| Group of | Total weight gain (kg) | Daily gain (g) | Number of heads with diarrhea |
| Example 1 | 51.63±2.31 | 573.57±23.54 | 0 |
| Example 2 | 53.17±1.67 | 590.39±26.12 | 0 |
| Example 3 | 52.53±1.94 | 583.11±27.31 | 0 |
| Comparative example 1 | 48.67±1.62 | 540.58±19.68 | 0 |
| Comparative example 2 | 48.23±1.77 | 535.12±25.22 | 0 |
| Comparative example 3 | 49.69±2.53 | 552.37±26.42 | 0 |
| Control group | 44.06±1.96 | 489.64±17.68 | 2 |
As can be seen from Table 1, when the feed additive for the ruminants obtained in the embodiments 1 to 3 of the present invention is added to a common feed, the total weight gain and the daily gain rate are both higher than those of a control group, and the diarrhea rate is greatly reduced, which indicates that the additive obtained in the technical scheme of the present invention has a good effect of improving the growth rate of the ruminants, and has a certain effect of regulating diarrhea, and the improvement effect is weak in the comparative examples and the comparative examples 1 to 3, which indicates that a synergistic effect exists between each enzyme and a microbial preparation.
The above-mentioned embodiments are only preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical scope of the present invention, and equivalents and modifications of the technical solutions and concepts of the present invention should be covered by the scope of the present invention.
Claims (6)
1. A feed additive for ruminants is characterized in that the additive comprises a complex enzyme preparation, a microbial preparation, a complex mineral substance and a diluent;
the enzyme activity of the compound enzyme preparation comprises the following components: 800-1600U/g cellulase, 3500-4500U/g xylanase and 1000-2000U/g pectinase;
the microbial preparation comprises the following components: bacillus subtilis at least 5 x 109CFU/g, bacillus licheniformis is more than or equal to 5 x 109CFU/g, yeast is more than or equal to 2 x 108CFU/g；
The compound mineral substance is a mixture of potassium iodide, ferrous sulfate and calcium phosphate;
the diluent is chitosan and zeolite powder.
2. The feed additive for ruminants according to claim 1, wherein the enzyme activity of the complex enzyme preparation comprises the following components: the cellulase is 1000-1500U/g, the xylanase is 3600-4200U/g, and the pectinase is 1200-1800U/g.
3. The feed additive for ruminants as claimed in claim 1, wherein the feed additive comprises the following raw materials in parts by weight:
15-30 parts of complex enzyme preparation, 10-30 parts of microbial preparation, 5-15 parts of complex mineral, 20-30 parts of chitosan and 10-20 parts of zeolite powder.
4. The feed additive for ruminants according to claim 1, wherein the microbial agent is bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and yeast and a culture thereof; the preparation method of the microbial preparation comprises the following steps:
s1, respectively carrying out activation culture on the bacillus subtilis strain, the bacillus licheniformis strain and the saccharomycete strain, and then carrying out amplification culture to obtain corresponding strains and culture solutions thereof;
s2, concentrating the strain obtained in the step S1 and a culture solution thereof, and freeze-drying to obtain bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and saccharomycetes and a culture thereof;
s3, mixing each strain obtained in S2 and the culture thereof to obtain the microbial preparation.
5. The preparation method of the feed additive for the ruminants is characterized by comprising the following steps of:
uniformly mixing cellulase, xylanase and pectinase to obtain a complex enzyme preparation;
respectively carrying out activation amplification culture on bacillus subtilis, bacillus licheniformis and saccharomycete, concentrating, freeze-drying corresponding culture solutions to obtain bacillus subtilis and a culture thereof, bacillus licheniformis and a culture thereof, and saccharomycete and a culture thereof, and mixing to obtain a microbial preparation;
and fully mixing the compound mineral, the chitosan, the zeolite powder, the compound enzyme preparation, the microbial preparation and the compound mineral to obtain the feed additive for the ruminant.
6. Use of the feed additive for any ruminant as prepared in claims 1-4 or claim 5, characterised in that the feed additive for ruminants is added in an amount of 300-500g per ton of feed.
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| CN113414226A (en) * | 2021-06-30 | 2021-09-21 | 云南丽天环保科技有限公司 | Kitchen waste leavening agent and preparation method and application thereof |
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