CN112304929A - Method and device for detecting biogenic amine in food materials - Google Patents
Method and device for detecting biogenic amine in food materials Download PDFInfo
- Publication number
- CN112304929A CN112304929A CN201910672704.6A CN201910672704A CN112304929A CN 112304929 A CN112304929 A CN 112304929A CN 201910672704 A CN201910672704 A CN 201910672704A CN 112304929 A CN112304929 A CN 112304929A
- Authority
- CN
- China
- Prior art keywords
- biogenic amine
- amine
- biogenic
- food
- sensitive material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
A method for detecting biogenic amine in food materials comprises the following steps: providing a biogenic amine detection element, wherein the biogenic amine detection element comprises a biogenic amine gas adsorption substrate, and the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporized gas or biogenic amine water vapor; disposing a biogenic amine sensitive material on the biogenic amine gas adsorption substrate; combining the biogenic amine detection element with a food material package to be detected or a food material to be detected; and generating and displaying a biogenic amine index by using the biogenic amine sensitive material immediately when the biogenic amine detecting element contacts a food material biogenic amine vaporized gas or biogenic amine water vapor.
Description
Technical Field
The invention relates to a method and a device for detecting biogenic amine (biogenic amine) in food materials; in particular to a non-contact method for detecting biogenic amine vaporized gas (monomer or vapor) of food materials and a device thereof; more particularly, to a method and an apparatus for detecting biogenic amine in food materials by using biogenic amine sensitive materials.
Background
For example, the present invention discloses a method for rapidly detecting biogenic amine, which is disclosed in the patent application of "a method for rapidly detecting biogenic amine" of Chinese patent publication No. CN-102650628. The method for rapidly detecting biogenic amine comprises the following steps: derivatizing a biological amine standard substance and a sample to be detected by using a derivatizing agent, then carrying out point application on a chromatography material polyamide film, then developing by using a developing agent, stopping chromatography when the front edge of the developing agent is 0.5-1 cm away from the top end of the film, generating color development, and observing the color development result.
In view of the above, CN-102650628 adopts a simple and rapid analysis method, which uses Thin Layer Chromatography (TLC) for determination, and when compared with other analysis methods, it has the advantages of no need of expensive analysis apparatus, low detection cost and rapid detection, but it still needs to extract and derivatize biogenic amine contained in food for detection and determination.
Another commonly used technology for detecting biogenic amine, for example, Chinese patent application No. CN-103149317, "a method for rapidly determining biogenic amine in food", discloses a method for rapidly determining biogenic amine in food. The method for rapidly determining biogenic amine in food comprises the following steps: 1. extracting biogenic amines from a food sample; 2. purifying a biological amine extracting solution; 3. derivatizing the biogenic amine extract; and 4, optimizing and analyzing by thin layer chromatography by using a developing agent.
In summary, the aforementioned CN-103149317 also adopts a simple and rapid analysis method, which also uses Thin Layer Chromatography (TLC) for determination, and when compared with other analysis methods, it has the advantages of no need of expensive analysis equipment, low detection cost and rapid detection, but it still needs to extract and derivatize biogenic amine contained in food for detection and determination.
Another commonly used technique for detecting biogenic amine, such as "a method for detecting histamine using an immunobiosensor" patent application of China patent publication No. CN-104897897, discloses a method for rapidly determining biogenic amine in food, comprising the steps of: s1, preparing an immune biosensor, and detecting the working curve of the sensor for standby; s2, preparing a group of amine monoclonal antibodies; s3, preparing an enzyme-labeled antibody; s4, histamine is detected by the sensor. And (3) uniformly mixing the same volume of a sample solution to be tested and the enzyme-labeled antibody prepared in the step (S3), dripping the mixture on an electrode working area, soaking the electrode in a test solution, adding hydrogen peroxide, and quantitatively determining the concentration of histamine according to the change of the front peak current value and the rear peak current value.
In summary, the method for detecting biogenic amine by using a biosensor in CN-104897897 has the advantages of low detection limit, high sensitivity, wide linear range, and applicability to field detection. However, the prior art of this method of biosensor detection of biogenic amines is still not very mature, there are few reliable kits on the market, and they are expensive.
Another commonly used technology for detecting biogenic amine, such as the invention patent of "portable meat freshness detector" in Taiwan patent publication No. TW-I467171, discloses a portable meat freshness detector. The portable meat freshness detector measures an impedance value and a capacitive reactance value of meat by a sensing unit, and calculates the measured impedance value and capacitive reactance value by an iterative operation program arranged in a processing unit to obtain an index parameter value; then, the index parameter value is compared with the standard parameter value recorded in the memory, so as to obtain the calculation result of the freshness of the meat, and the calculation result is displayed on a display unit.
In view of the above, the TW-I467171 th technique of calculating the impedance value and the capacitance value of the meat surface by contact measurement to obtain the freshness of the meat has advantages of no destruction of the meat, rapid detection, and convenient operation, but the contact measurement cannot be performed on the packaged food, and thus the freshness of the packaged food or the food material cannot be confirmed.
Another common technique for detecting biogenic amines, for example, the invention patent application of "cyclic aromatic functionalized gold nanoparticles and preparation method thereof" in chinese patent publication No. CN-104148626, discloses a cyclic aromatic functionalized gold nanoparticle and preparation method thereof. A gold nanoparticle modifies a cyclic aromatic hydrocarbon CEC4 to the surface of the gold nanoparticle through a gold-thiol bond, and the concentration ratio of the cyclic aromatic hydrocarbon CEC4 to the gold nanoparticle is 2000000: 23, the particle size of the gold nanoparticle is 13nm, and the CEC4 functionalized gold nanoparticle has specificity in the detection of biogenic amine.
In view of the above, CN-104148626 utilizes the color change of a compound reacted with biogenic amine, thereby detecting the freshness of food. Although this method has the advantage of simple and fast determination, it still needs to be in contact with the extraction liquid for reaction, which belongs to the contact or semi-contact measurement method. Since the contact measurement cannot be performed for the packaged food, the freshness of the packaged food or the food material cannot be confirmed.
Another commonly used technique for detecting biogenic amines, such as the Polymeric food choice sensor patent of U.S. patent publication No. US-6593142, discloses a food spoilage (choice) detector. The food deterioration (freshness) detector comprises a polymer (polymer) containing a composite material (polyaza macrococcus transition metal complex) which releases biogenic amine when contacting deteriorated food microorganisms (microorganisms) and generates color change (color change) after reacting with biogenic amine.
Accordingly, the aforementioned U.S. Pat. No. 4, 6593142 also utilizes the color change of a compound after reacting with biogenic amine, thereby detecting the freshness of food. Although this method has the advantage of simple and fast determination, it still needs to be in contact with the extraction liquid for reaction, which belongs to the contact or semi-contact measurement method. Since the contact measurement cannot be performed for the packaged food, the freshness of the packaged food or the food material cannot be confirmed.
Another commonly used biological amine adsorption removal technique, such as the invention patent application of "Biogenic amine oxidizer or nonreactive adsorbent" in U.S. Pat. No. 4, 2014/0087034, discloses a biological amine adsorbing oxidant (oxidazer). The biogenic amine adsorbing oxidant is used for adsorbing biogenic amine which removes food when the food is stored (storage) or packaged (packaging).
As mentioned above, the biogenic amine adsorption oxidant of the aforementioned US-2014/0087034 is only suitable for adsorbing biogenic amine for removing food, or it is suitable for reducing biogenic amine of food, but it is not used to show biogenic amine or freshness of food or food material.
Another commonly used biogenic amine adsorption removal technique, such as the "Sensores cromogenics para amides" patent application, Spanish patent publication No. ES-2557332, discloses a biogenic amine sensitive material. The film M1 and the film M1sen of the biogenic amine sensitive material adopt complex components and complex preparation operation.
In summary, the biogenic amine sensitive material of the aforementioned No. ES-2557332 has no biogenic amine gas adsorbing substrate and no predetermined adsorbing area, so as to bind with the biogenic amine sensitive material, and the biogenic amine sensitive material is not used for indicating biogenic amine or freshness of food or food materials.
Another commonly used technique for detecting biogenic amines, such as "chemical sensing hydrogel for biogenic amine detection" patent application of chinese patent publication No. CN-107037044, discloses a chemical sensing hydrogel for biogenic amine detection, i.e. it belongs to wet hydrogel or wet hydrogel mixture, and the wet hydrogel or wet hydrogel mixture needs to be combined with a container for packaging food in a sealing manner.
In view of the above, the wet hydrogel of the chemical sensing hydrogel for biogenic amine detection of CN-107037044 has the disadvantage of complicated manufacturing process, and the wet hydrogel does not have biogenic amine gas absorption substrate and predetermined absorption region thereof at all, and the biogenic amine sensitive material does not show biogenic amine or freshness of food or food material by dry substrate.
However, there is a need for further improvement of the aforementioned detection apparatus and method for the same in the aforementioned Chinese patent publication No. CN-102650628, Chinese patent publication No. CN-103149317, Chinese patent publication No. CN-104897897, Taiwan patent publication No. TW-I467171, Chinese patent publication No. CN-104148626, U.S. patent publication No. US-6593142, U.S. patent publication No. US-2014/0087034, Spanish patent publication No. ES-2557332, and Chinese patent publication No. CN-107037044.
The aforementioned chinese patent publication No. CN-102650628, chinese patent publication No. CN-103149317, chinese patent publication No. CN-104897897, taiwan patent publication No. TW-I467171, chinese patent publication No. CN-104148626, U.S. patent publication No. US-6593142, U.S. patent publication No. US-2014/0087034, spanish patent publication No. ES-2557332, and chinese patent publication No. CN-107037044 are only for reference of the background of the present invention and for illustrating the state of the art, and are not intended to limit the scope of the present invention.
In view of the above, the present invention provides a method and an apparatus for detecting biogenic amine in food materials, wherein a biogenic amine sensitive material is disposed on a biogenic amine gas adsorption substrate to form a biogenic amine detection element, and the biogenic amine detection element is combined with a food material package or a food material to be detected, and once the biogenic amine detection element contacts with a food material biogenic amine vapor or a biogenic amine vapor, the biogenic amine sensitive material is used to generate and display a biogenic amine index, so as to improve the technical disadvantages of the conventional method and apparatus for detecting biogenic amine in food materials.
Disclosure of Invention
The invention mainly aims to provide a method and a device for detecting biogenic amine in food materials, wherein a biogenic amine sensitive material is arranged on a biogenic amine gas adsorption base material to form a biogenic amine detection element, the biogenic amine detection element is combined with a food material package to be detected or a food material to be detected, and once the biogenic amine detection element contacts with food material biogenic amine vaporized gas or biogenic amine water vapor, the biogenic amine sensitive material is immediately utilized to generate and display a biogenic amine index, so that the aims of simplifying the operation of detecting the biogenic amine in the food materials, accelerating the detection efficiency and reducing the detection cost are fulfilled.
In order to achieve the above object, the apparatus for detecting biogenic amine in food according to the preferred embodiment of the present invention comprises:
a biogenic amine gas adsorption substrate, which is provided with a preset adsorption area, and the preset adsorption area of the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporized gas or biogenic amine water vapor;
a biogenic amine sensitive material which is configured on the biogenic amine gas adsorption base material to form a biogenic amine detection element; and
an element combining part which is arranged on the biogenic amine detection element and is combined with a food material package to be detected or food material to be detected;
wherein the biogenic amine sensitive material is used to generate a product exhibiting a biogenic amine index [ color change ] upon contact of the biogenic amine detecting element with a food material biogenic amine boil-off gas or biogenic amine vapor.
The biogenic amine gas adsorption substrate of the preferred embodiment of the present invention is selected from the group consisting of a water-absorbent substrate and a bio-compatible substrate.
The biogenic amine gas adsorption substrate of the preferred embodiment of the invention is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof.
The biogenic amine sensitive material of the preferred embodiment of the invention comprises a shape-imparting layer and a color-changing layer.
The biogenic amine sensitive material of the preferred embodiment of the invention is selected from a mononitrated benzene ring molecular material.
The molecular material of the nitrated benzene ring of the preferred embodiment of the present invention has one nitrated functional group, two nitrated functional groups or three nitrated functional groups.
In order to achieve the above object, a method for detecting biogenic amine in food material according to another preferred embodiment of the present invention comprises:
providing a biogenic amine detection element, wherein the biogenic amine detection element comprises a biogenic amine gas adsorption substrate, and the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporized gas or biogenic amine water vapor;
disposing a biogenic amine sensitive material on the biogenic amine gas adsorption substrate;
combining the biogenic amine detection element with a food material package to be detected or a food material to be detected; and
once the biogenic amine detection element contacts a food material biogenic amine boil-off gas or biogenic amine vapor, the biogenic amine sensitive material is immediately used to generate a product exhibiting a biogenic amine index [ color change ].
The biogenic amine index of the preferred embodiment of the present invention is a biogenic amine concentration, such as: 50ppm, 100ppm, 500ppm, 1000ppm or other concentrations.
The concentration of biogenic amine in the preferred embodiment of the present invention comprises a biogenic amine safe concentration, a biogenic amine warning concentration or a biogenic amine hazardous concentration.
The biogenic amine safe concentration of the preferred embodiment of the invention is less than 20ppm, the biogenic amine warning concentration is 20-50ppm, and the biogenic amine hazardous concentration is greater than 50 ppm.
The biogenic amine index of the preferred embodiment of the invention is a visible light discoloration indicator, such as: original as primary green, over 50ppm as dark red or orange brown.
The biogenic amine detection element of the preferred embodiment of the present invention produces a biogenic amine index which shows a biogenic amine index at least within 1 hour, or preferably at least within 30 minutes, or more preferably at least within 10 minutes, or more preferably at least within 5 minutes, or more preferably at least within 1 minute when contacted with food material biogenic amine boil-off gas or biogenic amine steam.
The biogenic amine detection element of the preferred embodiment of the invention comprises an element combining part, and the element combining part is combined with a food material package to be detected or food material to be detected.
The component combining portion of the preferred embodiment of the present invention is a pasting portion, a label patch, a combining buckle, a label buckle or a combining portion of other structures.
The invention has the beneficial effects that:
the invention provides a device and a method for detecting biogenic amine in food, wherein a biogenic amine sensitive material is arranged on a biogenic amine gas adsorption base material to form a biogenic amine detection element, the biogenic amine detection element is combined with a food package to be detected or a food to be detected, and once the biogenic amine detection element contacts with food biogenic amine vapor or biogenic amine water vapor, the biogenic amine sensitive material is immediately used for generating and displaying a biogenic amine index, so that the aims of simplifying the operation of detecting the biogenic amine in the food, accelerating the detection efficiency and reducing the detection cost are fulfilled.
Drawings
Fig. 1 is a schematic side view of a food material biogenic amine detection device according to a first preferred embodiment of the present invention.
Fig. 2 is a schematic flow chart of a method for detecting biogenic amine in food materials according to a preferred embodiment of the invention.
Fig. 3 is a schematic side view of a food material biogenic amine detection device according to a second preferred embodiment of the invention.
Fig. 4 is a schematic side view of a food material biogenic amine detection device according to a third preferred embodiment of the invention.
Fig. 5 is a schematic view of the food material biogenic amine detection device according to the preferred embodiment of the invention, wherein biogenic amine sensitive materials and biogenic amines act together.
Fig. 6 is a schematic chemical structure diagram of the food material biogenic amine detection device adopting biogenic amine sensitive materials and biogenic amine action mechanisms according to the preferred embodiment of the invention.
Fig. 7A is a schematic chemical structure diagram of a conjugated molecule of a first sensitive material adopted by the apparatus for detecting biogenic amine in food material according to the preferred embodiment of the invention.
Fig. 7B is a schematic chemical structure diagram of a conjugated molecule of a second sensitive material adopted by the apparatus for detecting biogenic amine in food materials according to the preferred embodiment of the invention.
Fig. 7C is a schematic chemical structure diagram of a conjugated molecule of a third sensitive material adopted by the apparatus for detecting biogenic amine in food materials according to the preferred embodiment of the invention.
Fig. 8A is a schematic chemical structure diagram of the food material biogenic amine detection device adopting the first control molecule material according to the preferred embodiment of the invention.
Fig. 8B is a schematic chemical structure diagram of the food material biogenic amine detection device adopting the second control molecule material according to the preferred embodiment of the invention.
Reference numerals
1: a biogenic amine gas adsorption substrate; 10: a device for detecting biogenic amine gas in food materials; 11: an element bonding portion; 11 a: combining a retaining ring; 2: a biogenic amine-sensitive material; 3: an adhesive layer; g1: a sensitive material; g2: a control molecule material; s1: a step of; s2: a step of; s3: a step of; s4: and (5) carrying out the following steps.
Detailed Description
In order that the present invention may be fully understood, the following detailed description of the preferred embodiments is given by way of example only, and not by way of limitation, with reference to the accompanying drawings.
The method and the device for detecting the biogenic amine in the food material can properly select automatic, semi-automatic or non-automatic processing treatment or be applied to the surfaces of various agricultural, forestry, fishery and animal husbandry products, processed products or byproducts thereof; furthermore, the apparatus for detecting biogenic amine in food material according to the preferred embodiment of the present invention can be suitably applied to various related agricultural, forestry, fishery, animal husbandry products, processed products thereof, byproducts thereof, or related industries, but is not intended to limit the scope of the present invention.
For example, the method and apparatus for detecting biogenic amine in food material according to the preferred embodiment of the present invention are suitable for various vegetable and fruit products (e.g., sweet potato leaves, water spinach, green pepper, watermelon, strawberry, mango, pineapple, grape, papaya, sweet potato, radish, carrot, eggplant, red pepper or other vegetable and fruit products), agricultural products (e.g., pericarp surface of agricultural products, exocarpium of beans, nut shell, cheese block surface or other agricultural products) or fresh products (e.g., meat, seafood or other fresh products), but are not intended to limit the scope of the present invention.
In the preferred embodiment of the present invention, the technical term "biogenic amine" or "food biogenic amine" includes amines emitted during the putrefaction of food materials such as "histamine", "putrescine" or "cadaverine", and the technical term "food biogenic amine gas" or "food biogenic amine vaporized gas" is defined as "food biogenic amine vapor", "food biogenic amine vaporized gas" or "food biogenic amine vaporized gas", but the scope of the present invention is not limited thereto.
Fig. 1 is a schematic side view of a food material biogenic amine detection device according to a first preferred embodiment of the present invention. Referring to fig. 1, a food material biogenic amine gas detection device 10 according to a first preferred embodiment of the present invention includes a biogenic amine gas adsorption substrate 1, a biogenic amine sensitive material 2 and a component combining portion 11, and the biogenic amine gas adsorption substrate 1, the biogenic amine sensitive material 2 and the component combining portion 11 are properly assembled to form the food material biogenic amine gas detection device 10.
Referring again to fig. 1, for example, the biogenic amine gas adsorption substrate 1 comprises a first surface (or upper surface, not labeled) and a second surface (or lower surface, not labeled). The biogenic amine gas adsorption substrate 1 has a predetermined adsorption area (e.g. open gas contact area), and the predetermined adsorption area of the biogenic amine gas adsorption substrate 1 is suitable for adsorbing biogenic amine vaporized gas (i.e. biogenic amine vaporized gas) or biogenic amine vapor (i.e. biogenic amine vapor).
Referring again to fig. 1, for example, the biogenic amine gas adsorbing substrate 1 can be selected from a water absorbent substrate or a bio-compatible substrate. Alternatively, the biogenic amine gas adsorption substrate 1 can be made of a pulp material, a cotton material, a sponge material, a textile material or any combination thereof (or other suitable material with water absorption or water vapor adsorption function) or a dry substrate (e.g., filter paper, cotton sheet or other suitable dry substrate, i.e., non-wet hydrogel substrate) to assist in adsorbing the biogenic amine gas.
Referring to fig. 1 again, for example, the biogenic amine sensitive material 2 is disposed on any suitable position (e.g., in a suitable manner) of the first surface of the biogenic amine gas adsorbing substrate 1 to form a biogenic amine detecting element or an element with biogenic amine detecting function. The biogenic amine sensitive material 2 can be selected to form a biogenic amine sensitive layer, and the biogenic amine sensitive layer comprises a first surface (or upper surface, not shown) and a second surface (or lower surface, not shown).
Referring to fig. 1 again, for example, the biogenic amine sensitive material 2 is a sensitive material that can react with biogenic amine, and the biogenic amine sensitive material 2 includes a shape-imparting layer and a color-changing layer (e.g., color-changing region, color-changing pattern, color-changing text or other color-changing design), and the color-changing layer includes a biogenic amine color-changing material. In addition, the biological amine sensitive material 2 can be selected from a mononitrated benzene ring molecular material or other various chemical substances capable of being combined and reacted with biological amine, and the nitrated benzene ring molecular material has one nitrated functional group, two nitrated functional groups, three nitrated functional groups or other materials with similar functional groups.
Referring to fig. 1 again, for example, the element combining portion 11 is disposed on the biogenic amine detecting element, and the element combining portion 11 is combined with a package of a food material to be detected or a food material to be detected (e.g., properly sleeved or disposed on the food material to be detected). In addition, the element combining portion 11 can be selectively disposed on any suitable position of the first surface or the second surface of the biogenic amine gas adsorption substrate 1.
Fig. 2 is a flow chart of a method for detecting biogenic amine in food material according to a preferred embodiment of the present invention, which comprises four main steps S1 to S4, but the steps are not limited to the order of the steps in the present invention, and the order of the steps in the preferred embodiment of the present invention can be modified, divided, added, combined or reduced without departing from the scope of the present invention.
Referring to fig. 1 and fig. 2, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention includes step S1: first, for example, one or more biogenic amine detection elements are provided in a suitable manner, and the biogenic amine detection element comprises the biogenic amine gas adsorption substrate 1, and the biogenic amine gas adsorption substrate 1 is used for adsorbing a biogenic amine vaporized gas.
Referring to fig. 1 and fig. 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention includes step S2: then, for example, the biogenic amine sensitive material 2 can be optionally pre-disposed (e.g., dip-coated, spray-coated, drop-coated or other technical means) on the biogenic amine gas adsorbing substrate 1 in a suitable manner so as to detect the freshness of the food material to be detected, its related reference index or other detection reference index.
Referring to fig. 1 and fig. 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention includes step S3: then, for example, in the food processing operation, or other food material packaging operation, the biogenic amine detection element can be selectively combined with any food material package to be detected (for example, the inner surface of the package, not shown) or any food material to be detected (for example, any part of the food material, not shown).
Referring to fig. 1 and fig. 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention includes step S4: then, for example, once the bio-amine detecting element contacts a food material bio-amine vaporization gas or a bio-amine vapor, the bio-amine sensitive material 2 is immediately used to generate a bio-amine index (color change) which is a visually recognizable change. The method for detecting biogenic amine in food materials in the preferred embodiment of the invention does not need to touch the food materials of biogenic amine or liquid seeped out of the biogenic amine, and the biogenic amine in the food materials can be detected only by absorbing the gas vaporized from the biogenic amine emitted by the food materials to be detected.
Referring again to fig. 1 and 2, for example, the biogenic amine index may be selected to be a change that is clearly discernible to the naked eye, such as: color change, transparency change, display of a warning symbol, display of warning text, or other discernible physical or chemical change.
Referring again to fig. 1 and 2, for example, the biogenic amine index can be selectively set to a biogenic amine concentration, such as: 50ppm, 100ppm, 500ppm, 1000ppm or other concentrations. For example, the U.S. Food and Drug Administration (FDA) stipulates that the histamine content of aquatic products should not exceed 50ppm, and once the histamine content reaches 500ppm, it is feared to harm human health.
Referring to fig. 1 and fig. 2 again, for example, the concentration of biogenic amine includes a safe concentration of biogenic amine, a warning concentration of biogenic amine, or a hazardous concentration of biogenic amine. The biogenic amine safe concentration is below 20ppm, the biogenic amine warning concentration is 20-50ppm, and the biogenic amine hazardous concentration is above 50 ppm. The biogenic amine index is a visible light discoloration indicator, such as: originally in the primary color green, more than 50ppm dark red or orange brown, but this is not intended to limit the scope of the invention.
For example, if the biogenic amine concentration of the food material to be detected is close to 50ppm (i.e., biogenic amine warning concentration), the food material to be detected can be selected as a lower rack food material, and the lower rack food material is further processed into another processed food material or processed food, so that improper waste of the food material caused by the excessive biogenic amine concentration of the food material to be detected is reduced.
The biogenic amine detection element of the preferred embodiment of the present invention produces a biogenic amine index which shows a biogenic amine index at least within 1 hour, or preferably at least within 30 minutes, or more preferably at least within 10 minutes, or more preferably at least within 5 minutes, or more preferably at least within 1 minute when contacted with food material biogenic amine boil-off gas or biogenic amine steam.
Fig. 3 is a schematic side view of a food material biogenic amine detection device according to a second preferred embodiment of the invention. Referring to fig. 3, compared to the first embodiment, the apparatus for detecting biogenic amine as food material according to the second preferred embodiment of the present invention is provided with at least one adhesive layer 3 on the second surface (or lower surface) or other positions (first surface or upper surface) of the biogenic amine gas adsorbing substrate 1.
Referring to fig. 3 again, for example, compared to the second embodiment, another preferred embodiment of the present invention is to arrange the adhesive layer 3 on the first surface (or the upper surface) of the biogenic amine sensitive material 2, and the biogenic amine gas adsorbing substrate 1 is used for adsorbing biogenic amine gas, biogenic amine vaporized gas or biogenic amine vapor.
Fig. 4 is a schematic side view of a food material biogenic amine detection device according to a third preferred embodiment of the invention. Referring to fig. 4, in a device for detecting biogenic amine as a food material according to a third preferred embodiment of the present invention, a combination retaining ring 11a is disposed on one side of the biogenic amine gas adsorbing substrate 1.
Referring to fig. 1 and 4, for example, according to another preferred embodiment of the present invention, the component combining portion 11 can be selected from a pasting portion, a label patch (suitable for various non-water-containing food materials), a label buckle (suitable for various aquatic products or various meat products), or other combining portions according to various requirements.
Fig. 5 is a schematic diagram of the food material biogenic amine detection device according to the preferred embodiment of the invention, wherein biogenic amine sensitive materials and biogenic amines act together. Referring to fig. 1 and 5, for example, the biogenic amine sensitive material 2 includes a sensitive material G1, a polymer carrier material and a control molecular material G2, and a predetermined blending ratio between the sensitive material G1 and the control molecular material G2 is set so that the biogenic amine sensitive material 2 can adjust and control a color change range according to the concentration of biogenic amine.
Referring to fig. 1 and 5 again, for example, the sensitive material G1 has a reactive group capable of reacting with biogenic amine and an organic color-changing group (i.e., a conjugated double bond) that is a conjugated double bond. The control molecular material G2 is used to control environmental value, and the control molecular material G2 has the property of binding with biogenic amine [ competition reaction ], thereby controlling the concentration of discoloration, as shown in the upper half of fig. 5. In addition, the polymer carrier material is suitable for dipping or spraying the biogenic amine sensitive material 2 on filter paper, cotton sheets or other suitable dry-type base materials of the biogenic amine gas adsorption base material 1.
Referring to fig. 5 again, for example, after biogenic amines (steam or gas) on food material are detected and combined with the reactive group of the sensitive material G1, as shown in the left side of fig. 5, electrons thereof are transmitted to the conjugated portion through the reactive group to generate a color change reaction, as shown in the middle portion of fig. 5, so that the conjugated double bond structure of the organic color change group is changed to finally cause a color change (e.g., change a predetermined color or change a shade) thereof, as shown in the right side of fig. 5.
Fig. 6 is a schematic chemical structure diagram of an apparatus for detecting biogenic amine in food according to a preferred embodiment of the present invention, which employs biogenic amine sensitive materials and biogenic amine action mechanisms, and corresponds to fig. 5. Referring to fig. 5 and 6, for example, when a biological amine (steam or gas) and the conjugated portion generate a substitution or addition reaction (as shown in the left side of fig. 6), the conjugated double bond conduction of the conjugated portion of the sensing material G1 causes the conjugated portion to generate a color change, the control molecule of the control molecule material G2 can control the color change range of the conjugated portion, and the polymer carrier material can absorb the volatile amine, fix the color change molecule of the conjugated portion and fix the control molecule.
Fig. 7A shows a schematic chemical structure diagram of a conjugated molecule of a first sensitive material G1, which corresponds to the conjugated portion of fig. 5, adopted by the food material biogenic amine detection apparatus according to the preferred embodiment of the present invention. Fig. 7B shows a schematic chemical structure diagram of a conjugated molecule of a second sensing material G1, which corresponds to the conjugated portion of fig. 5, adopted by the food material biogenic amine detection apparatus according to the preferred embodiment of the present invention. Fig. 7C shows a schematic chemical structure diagram of a conjugated molecule of a third sensing material G1, which corresponds to the conjugated portion in fig. 5, adopted by the food material biogenic amine detection apparatus according to the preferred embodiment of the present invention.
Fig. 8A shows a schematic chemical structure diagram of a food material biogenic amine detection device adopting a first control molecule material G2 according to a preferred embodiment of the present invention, which corresponds to the control molecule material in fig. 5. Fig. 8B shows a schematic chemical structure diagram of a second control molecule material G2 adopted by the food material biogenic amine detection device according to the preferred embodiment of the invention, which corresponds to the control molecule material in fig. 5.
The foregoing description of the preferred embodiments is merely exemplary of the invention and its technical features, and the techniques of the embodiments may be suitably modified and/or substituted in various substantially equivalent manners; therefore, the scope of the present invention is defined by the appended claims.
Claims (10)
1. An apparatus for detecting biogenic amine in food materials, comprising:
a biogenic amine gas adsorption substrate, which is provided with a preset adsorption area, and the preset adsorption area of the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporized gas or biogenic amine water vapor;
a biogenic amine sensitive material which is configured on the biogenic amine gas adsorption base material to form a biogenic amine detection element and comprises a sensitive material, a high molecular carrier material and a control molecular material, wherein the sensitive material is provided with a reaction group capable of reacting with biogenic amine and an organic color-changing group containing conjugated double bonds; and
an element combining part which is arranged on the biogenic amine detection element and is combined with a food material package to be detected or food material to be detected;
wherein the biogenic amine sensitive material is immediately utilized to generate and display a color change once a predetermined adsorption area of the biogenic amine gas adsorption substrate of the biogenic amine detection element is contacted with a food material biogenic amine vaporized gas or biogenic amine water vapor.
2. The food material biogenic amine detection device of claim 1, wherein the biogenic amine gas adsorption substrate is selected from a water-absorbing substrate or a bio-compatible substrate.
3. The food material biogenic amine detection device as claimed in claim 1, wherein the biogenic amine gas adsorption substrate is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof.
4. The food material biogenic amine detection device as claimed in claim 1, wherein the biogenic amine sensitive material comprises a shape-imparting layer and a color-changing layer.
5. The food material biogenic amine detection device as claimed in claim 1, wherein the biogenic amine sensitive material is selected from a mononitrated benzene ring molecular material.
6. A method for detecting biogenic amine in food materials, comprising:
providing a biological amine detection element, wherein the biological amine detection element comprises a biological amine gas adsorption substrate, and a preset adsorption area of the biological amine gas adsorption substrate is used for adsorbing biological amine vaporized gas or biological amine water vapor;
disposing a biogenic amine sensitive material on the biogenic amine gas adsorption substrate, wherein the biogenic amine sensitive material comprises a sensitive material, a high molecular carrier material and a control molecular material, the sensitive material is provided with a reaction group capable of reacting with biogenic amine and an organic color-changing group containing conjugated double bonds, after the biogenic amine vaporized gas or biogenic amine vapor is firstly combined with the reaction group, electrons of the biogenic amine vaporized gas or biogenic amine vapor are transferred to the conjugated part through the reaction group to generate color-changing reaction, and the control molecular material is combined with biogenic amine to further control the color-changing concentration;
combining the biogenic amine detection element with an element combination part on a food material package to be detected or a food material to be detected; and
once the preset adsorption area of the biogenic amine gas adsorption substrate of the biogenic amine detection element is contacted with food biogenic amine vaporized gas or biogenic amine water vapor, the biogenic amine sensitive material is immediately utilized to generate and display a color change.
7. The method of claim 6, wherein the biogenic amine index is a biogenic amine concentration.
8. The method as claimed in claim 7, wherein the biogenic amine concentration comprises a biogenic amine safe concentration, a biogenic amine warning concentration or a biogenic amine hazard concentration.
9. The method of claim 6, wherein the biogenic amine detection element comprises an element binding portion, and the element binding portion is bound to a package of food material to be detected or a food material to be detected.
10. The method as claimed in claim 9, wherein the component combining portion is a pasting portion, a label patch, a combining buckle, a label buckle or a combination of other structures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910672704.6A CN112304929B (en) | 2019-07-24 | 2019-07-24 | Method and device for detecting biogenic amine in food material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910672704.6A CN112304929B (en) | 2019-07-24 | 2019-07-24 | Method and device for detecting biogenic amine in food material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112304929A true CN112304929A (en) | 2021-02-02 |
| CN112304929B CN112304929B (en) | 2023-10-27 |
Family
ID=74328799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910672704.6A Active CN112304929B (en) | 2019-07-24 | 2019-07-24 | Method and device for detecting biogenic amine in food material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112304929B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030100118A1 (en) * | 2000-04-10 | 2003-05-29 | Kelly Craig A. | Polymeric food spoilage sensor |
| US20040072359A1 (en) * | 2002-09-19 | 2004-04-15 | Southard Glen E. | Sensor for monitoring an analyte |
| MXPA05007224A (en) * | 2002-12-30 | 2005-09-12 | Kimberly Clark Co | Sensor for monitoring an analyte. |
| WO2006024848A1 (en) * | 2004-09-01 | 2006-03-09 | Cranfield University | Food spoilage sensor |
| US20080299669A1 (en) * | 2007-03-23 | 2008-12-04 | Lavigne John J | Methods and devices for analytical sensing of biogenic amines |
| US20110306140A1 (en) * | 2010-06-10 | 2011-12-15 | City University Of Hong Kong | Molecular Heterobimetallic Chemodosimetric Sensor for the Detection of Biogenic Amines |
| CN102650628A (en) * | 2012-05-18 | 2012-08-29 | 中国农业大学 | Method for quickly detecting biogenic amine |
| US20140087034A1 (en) * | 2012-09-25 | 2014-03-27 | Multisorb Technologies, Inc. | Biogenic amine oxidizer or unreactive absorber |
| CN107037044A (en) * | 2016-02-01 | 2017-08-11 | 香港城市大学 | Chemical sensitisation hydrogel for determination of biogenic amines |
| CN107300553A (en) * | 2017-07-04 | 2017-10-27 | 江苏大学 | It is a kind of based on can storage type gas sensor piscine organism amine content detection method |
| CN109946349A (en) * | 2019-04-02 | 2019-06-28 | 武汉轻工大学 | Organic field effect tube and preparation method thereof and biogenic amine gas sensor |
-
2019
- 2019-07-24 CN CN201910672704.6A patent/CN112304929B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030100118A1 (en) * | 2000-04-10 | 2003-05-29 | Kelly Craig A. | Polymeric food spoilage sensor |
| US20040072359A1 (en) * | 2002-09-19 | 2004-04-15 | Southard Glen E. | Sensor for monitoring an analyte |
| MXPA05007224A (en) * | 2002-12-30 | 2005-09-12 | Kimberly Clark Co | Sensor for monitoring an analyte. |
| WO2006024848A1 (en) * | 2004-09-01 | 2006-03-09 | Cranfield University | Food spoilage sensor |
| US20080299669A1 (en) * | 2007-03-23 | 2008-12-04 | Lavigne John J | Methods and devices for analytical sensing of biogenic amines |
| US20110306140A1 (en) * | 2010-06-10 | 2011-12-15 | City University Of Hong Kong | Molecular Heterobimetallic Chemodosimetric Sensor for the Detection of Biogenic Amines |
| CN102650628A (en) * | 2012-05-18 | 2012-08-29 | 中国农业大学 | Method for quickly detecting biogenic amine |
| US20140087034A1 (en) * | 2012-09-25 | 2014-03-27 | Multisorb Technologies, Inc. | Biogenic amine oxidizer or unreactive absorber |
| CN107037044A (en) * | 2016-02-01 | 2017-08-11 | 香港城市大学 | Chemical sensitisation hydrogel for determination of biogenic amines |
| CN107300553A (en) * | 2017-07-04 | 2017-10-27 | 江苏大学 | It is a kind of based on can storage type gas sensor piscine organism amine content detection method |
| CN109946349A (en) * | 2019-04-02 | 2019-06-28 | 武汉轻工大学 | Organic field effect tube and preparation method thereof and biogenic amine gas sensor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112304929B (en) | 2023-10-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Determination of phthalate acid esters plasticizers in plastic by ultrasonic solvent extraction combined with solid-phase microextraction using calix [4] arene fiber | |
| Balasubramanian et al. | Solid-phase microextraction (SPME) techniques for quality characterization of food products: a review | |
| Eskilsson et al. | Analytical-scale microwave-assisted extraction | |
| CA1137333A (en) | Self-contained gaseous contaminant dosimeter | |
| Ahmad et al. | Leaching of styrene and other aromatic compounds in drinking water from PS bottles | |
| Wzorek et al. | Application of GC-MS with a SPME and thermal desorption technique for determination of dimethylamine and trimethylamine in gaseous samples for medical diagnostic purposes | |
| US6043096A (en) | Device and method for the determination of water | |
| Hashemian et al. | Cellulose acetate/MOF film-based colorimetric ammonia sensor for non-destructive remote monitoring of meat product spoilage | |
| Zheng et al. | A review on rapid detection of modified quartz crystal microbalance sensors for food: Contamination, flavour and adulteration | |
| Del Nobile et al. | Water transport properties of cellophane flexible films intended for food packaging applications | |
| Souza-Silva et al. | Recent advances in solid-phase microextraction for contaminant analysis in food matrices | |
| CN112304929A (en) | Method and device for detecting biogenic amine in food materials | |
| CN104749175A (en) | Method for detecting organophosphorus pesticide residues in fruits and vegetables | |
| Geramizadegan et al. | Molecularly imprinted polymers for selective extraction and determination of toxic herbicide bentazon in water samples using liquid chromatography and assessment of mean square error using artficial neural network model | |
| Bang et al. | Sol–gel‐adsorbent‐coated extraction needles to detect volatile compounds in spoiled fish | |
| TWI714821B (en) | Method and device for detecting biogenic amine of food materials | |
| TWI767092B (en) | Simplified method and device for detecting biogenic amine of food materials | |
| US10126259B2 (en) | Radio frequency identification (RFID) devices for detecting volatile substances | |
| EP3803321A1 (en) | Devices and methods for collecting and storing fluid samples for analysis | |
| Kudlejova et al. | Application of solid-phase microextraction in food and fragrance analysis | |
| CN110132782A (en) | A kind of activated carbon fibre Low Concentration of Benzene Dynamic Adsorption value detection method | |
| Pournamdari et al. | Assessment and selective extraction megestrol drug by molecularly imprinted polymers method in human fluid samples using liquid chromatography | |
| CN108828114A (en) | A kind of method of solid phase microextraction-mass spectrometry on-line preconcentration detection Performance Liquid Chromatography Analysis for Nicotine in Tobacco class compound | |
| Ding et al. | Molecularly imprinted solid‐phase extraction of epicatechin from tea beverage | |
| EP2247934A1 (en) | Modified cartridge with adsorbent polymer for solid-phase extraction ( spe) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |