CN112300965A - Strain DDZR-YB001 and application thereof in high-temperature fermentation degradation of antibiotics - Google Patents
Strain DDZR-YB001 and application thereof in high-temperature fermentation degradation of antibiotics Download PDFInfo
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- CN112300965A CN112300965A CN202011226596.9A CN202011226596A CN112300965A CN 112300965 A CN112300965 A CN 112300965A CN 202011226596 A CN202011226596 A CN 202011226596A CN 112300965 A CN112300965 A CN 112300965A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Business, Economics & Management (AREA)
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Abstract
The invention provides a strain DDZR-YB001 and application thereof in high-temperature fermentation and degradation of antibiotics, wherein the strain DDZR-YB001 is preserved in the China general microbiological culture Collection center in 2016, 8, 15 and the preservation number is CGMCC NO. 12847; soil and human and animal excreta containing high-dose antibiotics are uniformly mixed with the strain DDZR-YB001 according to the weight ratio of 1: 1; ventilating and fermenting at 90-100 deg.C for 42 days, and turning over the tank every 7 days. The invention can promote the antibiotic content in soil and human and animal excreta to be obviously reduced, part of antibiotic is completely decomposed and the degradation rate can reach more than 99.7 percent after fermentation is finished by high-temperature fermentation for 42 days.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a strain DDZR-YB001 and application thereof in high-temperature fermentation and degradation of antibiotics.
Background
In recent years, there are many reports about antibiotic pollutants detected in water, sediments and soil at home and abroad, and even antibiotic residues are found in products such as vegetables, milk, meat and the like. In order to solve the problem of antibiotic contamination, in addition to reducing the abuse of antibiotics, how to remove residual antibiotics in environmental systems has become a hot spot of research in recent years.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a strain DDZR-YB001 and application thereof in high-temperature fermentation and antibiotic degradation so as to degrade antibiotics in soil and human and animal excreta.
In order to achieve the aim, the invention provides a strain DDZR-YB001, wherein the strain DDZR-YB001 is preserved in the China general microbiological culture Collection center in 2016, 8, 15 and the preservation number is CGMCC NO. 12847.
As a further illustration of the strain DDZR-YB001, preferably, the strain DDZR-YB001 is a Bacillus subtilis.
In order to realize another purpose of the invention, the invention also provides application of the strain DDZR-YB001 in fermentation degradation of degradation products of antibiotic pollutants.
As a further illustration of the application of the invention, preferably, soil and human and animal excreta containing 100-150ppb antibiotics are uniformly mixed with the strain DDZR-YB001 in the weight ratio of 1:1-1.2, and are subjected to ventilation fermentation at the fermentation temperature of 90-100 ℃ for 10-15m3In terms of hours. More preferably, the hair is ventilatedThe fermentation time was 42 days.
As a further illustration of the use according to the invention, preferably the antibiotic is selected from the group consisting of ofloxacin (ofc)), ciprofloxacin (cfc)), enrofloxacin (efc), diflunisal (difc), chloramphenicol (chlorohexitol (cpc), lincomycin (lim), sulfadimetrazine (smz), sulfamonomethoxine (smm), sulfaquinoxaline (sxx sql), sulfadimethoxine (sdm).
As a further illustration of the use according to the invention, it is preferred that the tank turnover treatment is carried out every 7 days during the fermentation.
The strain DDZR-YB001 of the invention can promote the antibiotic content in the soil and the human and animal excreta to be obviously reduced after the fermentation is finished by fermenting the soil and the human and animal excreta containing high-dose antibiotic for 42 days, and the degradation rate can reach more than 99.7 percent.
Detailed Description
The present invention will be further described with reference to the following examples, which are only for illustrating the technical solutions of the present invention and are not to be construed as limiting the present invention.
The invention provides a strain DDZR-YB001, wherein the strain DDZR-YB001 is preserved in the China general microbiological culture Collection center in 2016, 8, 15 and the preservation number is CGMCC NO. 12847.
Example 1
Firstly, 200kg of soil and human and animal excreta containing high dose (100ppb) antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflunisal, chloramphenicol, lincomycin, sulfamethazine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine are respectively taken for detection, the types of the antibiotics contained in the soil and the human and animal excreta are detected, then 200kg of strain DDZR-YB001 is taken to uniformly mix the soil and the excrement of human and animals containing high-dose antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflufenic, chloramphenicol, lincomycin, sulfadimidine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine with the strain DDZR-YB001 according to the weight ratio of 1:1, ventilating and fermenting for 42 days at the fermentation temperature of 90 ℃, turning the tank once every 7 days, and A, B, C three points were randomly selected as sampling points to detect the content change of each antibiotic.
Example 2
Firstly, 200kg of soil and human and animal excreta containing high dose (150ppb) antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflunisal, chloramphenicol, lincomycin, sulfamethazine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine are respectively taken for detection, the type and the content of the antibiotics are detected, then 200kg of strain DDZR-YB001 is taken to uniformly mix the soil and the excrement of human and animals containing high-dose antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflufenic, chloramphenicol, lincomycin, sulfadimidine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine with the strain DDZR-YB001 according to the weight ratio of 1:1, ventilating and fermenting for 42 days at the fermentation temperature of 95 ℃, turning over the tank once every 7 days, and A, B, C three points were randomly selected as sampling points to detect the content change of each antibiotic.
Example 3
Firstly, 200kg of soil and human and animal excreta containing high dose (125ppb) antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflunisal, chloramphenicol, lincomycin, sulfamethazine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine are respectively taken for detection, the type and the content of the antibiotics are detected, then 200kg of strain DDZR-YB001 is taken to uniformly mix the soil and the excrement of human and animals containing high-dose antibiotics ofloxacin, ciprofloxacin, enrofloxacin, diflufenic, chloramphenicol, lincomycin, sulfadimidine, sulfamonomethoxine, sulfaquinoxaline or sulfadimethoxine with the strain DDZR-YB001 according to the weight ratio of 1:1, ventilating and fermenting for 42 days at 100 deg.C, turning over the tank once every 7 days, and A, B, C three points were randomly selected as sampling points to detect the content change of each antibiotic.
Example 4
Tables 1 to 3 below show the experimental data of the average value of the variation in the content of each antibiotic obtained by testing A, B, C samples of examples 1 to 3. Wherein: table 1 shows the average value of the change in the content of each antibiotic at the sampling points A in examples 1 to 3; table 2 shows the average value of the variation of the content of each antibiotic at the B sampling points in examples 1-3; table 3 shows the average values of the antibiotic content at the C sampling points in examples 1-3.
TABLE 1
Table 2 shows the mean change in the content of each antibiotic at the B-sampling points in examples 1 to 3
Table 3 shows the mean change in the content of each antibiotic at the C-sampling points in examples 1 to 3
As can be seen from tables 1 to 3, the antibiotics detected in the soil and the human and animal excreta containing high-dose antibiotics include ofloxacin, ciprofloxacin, enrofloxacin, difloxacin, chloramphenicol, lincomycin, sulfadimidine, sulfamonomethoxine, sulfaquinoxaline and sulfadimethoxine, and the antibiotics are fermented at high temperature for up to 42 days by mixing with the strain DDZR-YB001, so that the concentration content is remarkably reduced after the fermentation is completed, and partial antibiotics are even completely decomposed.
Furthermore, a large number of experiments prove that the degradation rate of the strain DDZR-YB001 inoculated into a culture medium of any group of ofloxacin, ciprofloxacin, enrofloxacin, diflunisal, chloramphenicol, lincomycin, sulfadimidine, sulfamonomethoxine, sulfaquinoxaline and sulfadimethoxine can still reach more than 99.7%.
It should be noted that the above summary and the detailed description are intended to demonstrate the practical application of the technical solutions provided by the present invention, and should not be construed as limiting the scope of the present invention. Various modifications, equivalent substitutions, or improvements may be made by those skilled in the art within the spirit and principles of the invention. The scope of the invention is to be determined by the appended claims.
Claims (7)
1. A strain DDZR-YB001 is characterized in that the strain DDZR-YB001 is preserved in the China general microbiological culture Collection center in 2016, 8, 15 and the preservation number is CGMCC No. 12847.
2. The strain DDZR-YB001 according to claim 1, wherein said strain DDZR-YB001 is bacillus subtilis.
3. Use of the strain DDZR-YB001 according to claim 1 or 2 in the fermentation of degradation products that degrade antibiotic-type contaminants.
4. The use as claimed in claim 3, characterized in that the soil and the human and animal excreta containing 100-150ppb antibiotics are mixed uniformly with the strain DDZR-YB001 in a weight ratio of 1:1-1.2, and are subjected to fermentation with aeration at a fermentation temperature of 90-100 ℃ for 10-15m3In terms of hours.
5. The use of claim 4, wherein the time period for the aeration fermentation is 42 days.
6. The use of claim 4, wherein the antibiotic is selected from the group consisting of ofloxacin, ciprofloxacin, enrofloxacin, diflufenic acid, chloramphenicol, lincomycin, sulfamethazine, sulfamonomethoxine, sulfaquinoxaline, sulfadimethoxine.
7. Use according to any one of claims 4 to 6, wherein the tumbling treatment is carried out every 7 days during the fermentation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011226596.9A CN112300965B (en) | 2020-11-05 | 2020-11-05 | Strain DDZR-YB001 and application thereof in high-temperature fermentation degradation of antibiotics |
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| CN202011226596.9A CN112300965B (en) | 2020-11-05 | 2020-11-05 | Strain DDZR-YB001 and application thereof in high-temperature fermentation degradation of antibiotics |
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| CN112300965B CN112300965B (en) | 2022-09-30 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6391618B1 (en) * | 2001-03-01 | 2002-05-21 | Ultra Biotech Limited | Methods and compositions for degrading environmental toxins |
| CN102731177A (en) * | 2012-07-02 | 2012-10-17 | 深圳市芭田生态工程股份有限公司 | Two-step bioremediation method of organic pollutants in compost organic raw materials |
| CN108251331A (en) * | 2017-12-29 | 2018-07-06 | 中国农业科学院农业环境与可持续发展研究所 | Water balsamine Bacillus alcaligenes and microbial bacterial agent and their applications in animal waste recycling |
| CN108641984A (en) * | 2018-05-18 | 2018-10-12 | 北京农业生物技术研究中心 | One plant of urea bacillus SC-7 and its application in compost |
| CN109022318A (en) * | 2018-08-15 | 2018-12-18 | 河南道地生物科技有限公司 | Bacillus subtilis L1 and its application that lincomycin is remained in degradation lincomycin fungi residues |
| CN109293411A (en) * | 2018-11-01 | 2019-02-01 | 河北省微生物研究所 | The preparation method of microorganism-decomposing agent and its application in reduction feces of livestock and poultry antibiotic residue |
-
2020
- 2020-11-05 CN CN202011226596.9A patent/CN112300965B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6391618B1 (en) * | 2001-03-01 | 2002-05-21 | Ultra Biotech Limited | Methods and compositions for degrading environmental toxins |
| CN102731177A (en) * | 2012-07-02 | 2012-10-17 | 深圳市芭田生态工程股份有限公司 | Two-step bioremediation method of organic pollutants in compost organic raw materials |
| CN108251331A (en) * | 2017-12-29 | 2018-07-06 | 中国农业科学院农业环境与可持续发展研究所 | Water balsamine Bacillus alcaligenes and microbial bacterial agent and their applications in animal waste recycling |
| CN108641984A (en) * | 2018-05-18 | 2018-10-12 | 北京农业生物技术研究中心 | One plant of urea bacillus SC-7 and its application in compost |
| CN109022318A (en) * | 2018-08-15 | 2018-12-18 | 河南道地生物科技有限公司 | Bacillus subtilis L1 and its application that lincomycin is remained in degradation lincomycin fungi residues |
| CN109293411A (en) * | 2018-11-01 | 2019-02-01 | 河北省微生物研究所 | The preparation method of microorganism-decomposing agent and its application in reduction feces of livestock and poultry antibiotic residue |
Non-Patent Citations (1)
| Title |
|---|
| 王强锋等: "三种农用抗生素降解真菌的筛选及其降解性能", 《农业资源与环境学报》 * |
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