CN112285064A - SPR sensor, single cell detection device and single cell detection method based on SPR - Google Patents
SPR sensor, single cell detection device and single cell detection method based on SPR Download PDFInfo
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- 239000002184 metal Substances 0.000 claims abstract description 21
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- 238000012576 optical tweezer Methods 0.000 claims description 9
- 239000013307 optical fiber Substances 0.000 claims description 5
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- 239000003574 free electron Substances 0.000 description 1
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- 230000009878 intermolecular interaction Effects 0.000 description 1
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G—PHYSICS
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
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- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
- G01J2003/425—Reflectance
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
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Abstract
The invention discloses an SPR sensor which comprises a metal film, wherein a second surface of the metal film is provided with a cell to-be-detected position for placing a cell to be detected, an inclined surface is arranged on a position, opposite to the cell to-be-detected position, of a first surface of the metal film opposite to the second surface, and the inclined surface has a second slope different from a first slope of the first surface. The accuracy of single cell detection is improved by the invention.
Description
Technical Field
The invention relates to the technical field of optical biochemical sensing, in particular to an SPR sensor, a single cell detection device based on SPR and a single cell detection method based on SPR.
Background
Single cell research is one of the hot spots of great interest in the biomedical field today. Traditional biology is used for identifying cells, and marking modes such as staining are often needed, so that the cells are damaged and even die, and further analysis and application of the same specific cells are limited. Based on SPR (Surface Plasmon Resonance) detection technology, the method has the advantages of real-time monitoring, no need of marking samples, few samples, convenient and quick detection process and higher sensitivity.
The SPR sensor uses evanescent waves generated by total reflection of light on a medium and a metal interface to trigger collective oscillation of free electrons on the metal surface so as to generate surface plasma waves, when the wavelength or the incident angle of incident light is a certain proper value, the wave vector component of the incident light along the interface is equal to the wave vector of the surface plasma waves, the incident light and the surface plasma waves resonate, the energy of the incident light is absorbed, the energy of the reflected light is rapidly reduced, a lowest intensity value, namely an SPR resonance peak, appears on the intensity spectrum of the reflected light, the resonance angle changes along with the change of the refractive index of the medium on the surface of the metal film, and the change of the refractive index is in direct proportion to the mass of molecules combined on the metal surface. Thus, by analyzing the resonance angle, information on the intermolecular interaction can be obtained.
However, when the conventional SPR sensor is used for single cell detection, if the spot of incident light emitted from the light source is small, the light is difficult to be accurately incident to the position of the single cell, which causes inaccurate cell detection; if the light spot of the incident light emitted by the light source is too large and the single cell is too small, most of background noise exists in the acquired spectral information, and the background noise affects the accuracy of cell detection. Therefore, the existing traditional SPR sensor has certain difficulty and inaccuracy in the control and detection of single cells.
Disclosure of Invention
Accordingly, the present invention is directed to provide an SPR sensor, and an SPR-based single cell detection apparatus and method, which solve the above-mentioned problems and improve the accuracy of single cell detection.
In order to achieve the above object, the present invention provides an SPR sensor including a metal film, a cell to-be-detected spot on a second surface of the metal film for placing a cell to be detected, and an inclined surface having a second slope different from a first slope of a first surface of the metal film opposite to the second surface and facing the cell to-be-detected spot on the first surface.
Preferably, the first slope and the second slope simultaneously satisfy that total reflection can occur when incident light irradiates the first surface.
Preferably, the size of the cell to be mapped matches the size of an individual cell.
Preferably, the area of the inclined surface projected onto the second surface matches the area of the cell to be mapped.
In order to achieve the above object, the present invention provides a single cell detection device based on SPR, comprising the SPR sensor as described above, a light source, fiber optical tweezers, a microscope and a first receiving device;
the optical fiber tweezers are used for placing a single cell to be detected in a cell to-be-detected position of the SPR sensor;
the microscope is used for controlling the fiber optical tweezers to move the single cell to be detected under the observation of the microscope;
the light source is used for projecting detection light rays onto the SPR sensor, and the projection position of the detection light rays meets the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor;
the first receiving device is used for receiving the first SPR reflected light reflected by the inclined plane of the SPR sensor.
Preferably, the first receiving device is further configured to receive a second SPR light reflected by the surface of the SPR sensor when no single cell to be detected is placed on the spot to be detected of the cell of the SPR sensor.
Preferably, the detection device further comprises a second receiving device, when the cell to be detected is placed on the cell to be detected of the SPR sensor, the first receiving device is used for receiving the first SPR reflected light reflected by the inclined plane of the SPR sensor, and the second receiving device is used for simultaneously receiving the second SPR light reflected by the surface of the SPR sensor.
In order to achieve the above object, the present invention provides a single cell detection method based on SPR, comprising:
placing the single cell to be detected on the cell to be detected position of the SPR sensor under a microscope through optical fiber tweezers;
projecting detection light rays onto the SPR sensor, wherein the projection positions of the detection light rays meet the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor;
the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor.
Preferably, the method comprises a step-by-step detection method, and specifically comprises:
the first step, when no single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a second SPR reflected light ray reflected by the surface of the SPR sensor;
and secondly, when the single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a first SPR reflected light ray reflected by the inclined plane of the SPR sensor.
Preferably, the method for synchronously detecting specifically comprises: when the cell to be detected is placed on the cell to be detected position of the SPR sensor, the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor, and the second receiving device simultaneously receives the second SPR reflected light reflected by the surface of the SPR sensor.
Compared with the prior art, the SPR sensor, the single cell detection device based on SPR and the single cell detection method have the following beneficial effects: the position of the single cell can be accurately positioned; even if the light spot of the incident light source is large, the reflected light signal of the position of the single cell can be accurately captured, and the single cell detection precision is improved; the scheme is simple in design and simple and convenient to operate, and can measure and analyze the spectral information of the single cells in real time without damage.
Drawings
FIG. 1 is a system schematic of a SPR sensor according to one embodiment of the present invention.
FIG. 2 is a schematic diagram of an SPR sensor according to one embodiment of the present invention.
FIG. 3 is a light ray schematic of a SPR sensor according to one embodiment of the present invention.
FIG. 4 is a system schematic of a single cell detection device based on SPR in accordance with one embodiment of the present invention.
FIG. 5 is a schematic flow diagram of a SPR-based single cell detection method according to one embodiment of the present invention.
Detailed Description
The present invention will be described in detail with reference to the specific embodiments shown in the drawings, which are not intended to limit the present invention, and structural, methodological, or functional changes made by those skilled in the art according to the specific embodiments are included in the scope of the present invention.
In an embodiment of the present invention as shown in fig. 1, the present invention provides an SPR sensor including a metal film 01, a cell site 03 for placing a cell to be detected is disposed on a second surface 02 of the metal film, an inclined surface 05 is disposed on a first surface 04 of the metal film opposite to the second surface, the inclined surface 05 having a second slope different from a first slope of the first surface, and the first slope of the inclined surface is opposite to the cell site.
The traditional SPR sensor is provided with the inclined plane, when light spots of light beams of the light source irradiate the surface of the SPR sensor, reflected light reflected by the light incident on the inclined plane is separated from the reflected light incident on the surface to form two SPR reflected light spots, so that the single cell can be accurately detected even if the light spots of the light beams of the light source are large in single cell detection by using the SPR sensor.
The second surface of the metal film of the SPR sensor is provided with a cell site to be detected, and the labeled cell site to be detected is used for placing a cell to be detected, as shown in fig. 2. The metal film is a 50nm metal film with a coated surface. The substrate of the SPR sensor may be formed of a thin layer of glass. And an inclined surface is arranged on the first surface of the metal film opposite to the second surface, the inclined surface is opposite to the position of the cell to be measured, and the inclined surface has a second slope different from the first slope of the first surface. The first slope and the second slope simultaneously meet the requirement that incident light can be totally reflected when irradiating the first surface, and light emitted by the light source is incident on the SPR sensor to form two beams of SPR reflected light. As shown in fig. 3. Therefore, when the light spot of the incident light source is large, the reflected light spot of the position where the single cell is located can be accurately received, and the single cell detection precision can be improved. The size of the cell to be mapped matches the size of the individual cells. The area of the tilted surface projected onto the second surface matches the area of the cell to be mapped.
In one embodiment of the invention as shown in fig. 4, the invention provides a single cell detection device based on SPR, comprising an SPR sensor 40 as described above, a light source 41, fiber optical tweezers 42, a microscope 43 and a first receiving device 44,
the optical fiber tweezers 42 are used for placing the single cell to be detected in the cell to-be-detected position of the SPR sensor 40;
the microscope 43 is used for controlling the fiber optical tweezers 42 to move the single cell to be detected under the observation of the microscope 43;
the light source 41 is used for projecting detection light rays onto the SPR sensor 40, and the projection positions of the detection light rays meet the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor 40;
the first receiving device 44 is used for receiving the first SPR reflected light reflected by the inclined plane of the SPR sensor 40.
The SPR sensor is marked with cells to be detected on a metal film and can be observed by a microscope. Under the assistance of a microscope and under the control of the fiber optical tweezers, placing the single cell to be detected on the position to be detected of the cell. The light source emits detection light, the detection light enters the SPR sensor, and the projection position of the detection light is enabled to meet the requirement that two beams of SPR reflection light can be formed when the detection light is reflected from the SPR sensor by adjusting the position of the light source. The first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor. When the spot of the light source is irradiated on the SPR sensor during single cell detection, reflected light reflected by the light incident on the inclined surface is separated from the reflected light incident on the surface to form two SPR reflection spots, so that the single cell can be accurately detected even if the spot of the light beam of the light source is large.
The detection device in this embodiment only includes the first receiving device, detection needs to be performed step by step when detecting a single cell, the first receiving device needs to collect SPR optical signals in two steps, in the first step, no single cell to be detected is placed on the position to be detected of the cell, and when a first beam of light emitted by the light source is incident on the surface of the SPR sensor, the first receiving device receives a second SPR light reflected by the surface of the SPR sensor; and secondly, placing a single cell to be detected on the position to be detected of the cell, wherein when a second beam of light emitted by the light source enters the surface of the SPR sensor, the first receiving device receives a first SPR reflected light ray reflected by an inclined plane of the SPR sensor, the inclined plane is opposite to the position to be detected of the cell, and the spectral information of the first SPR reflected light ray comprises the reflectivity of a single cell medium. And performing spectral analysis according to the received first SPR reflected light signal and the second SPR reflected light signal to acquire the analysis information of the single cell. The first receiving device comprises a first CCD camera.
According to a specific embodiment of the present invention, in the above embodiment, the reflected light signal needs to be acquired in two steps, which results in slow data acquisition and low efficiency. The detection device further comprises a second receiving device 45, when the cell to be detected is placed on the position to be detected of the cell of the SPR sensor, the first receiving device receives a first SPR reflected light ray reflected by an inclined plane of the SPR sensor, the inclined plane is opposite to the position to be detected of the cell, and the spectral information of the first SPR reflected light ray comprises the reflectivity of the single-cell medium. The second receiving device simultaneously receives second SPR light rays reflected by the SPR sensor surface, wherein the second SPR light rays comprise spectral information of a background around the single cell. And performing spectral analysis according to the received first SPR reflected light signal and the second SPR reflected light signal to acquire the analysis information of the single cell. The second receiving device comprises a second CCD camera. By using the first receiving device and the second receiving device, SPR reflected light signals can be synchronously collected, and the single cell detection efficiency is improved.
In one embodiment of the present invention as shown in fig. 5, the present invention provides a single cell detection method based on SPR, the method comprising:
s501, placing the single cell to be detected on the cell to be detected position of an SPR sensor under a microscope through fiber optical tweezers;
s502, projecting detection light rays onto the SPR sensor, wherein the projection positions of the detection light rays meet the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor;
s503, the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor.
Under the assistance of a microscope and under the control of the fiber optical tweezers, placing the single cell to be detected on the position to be detected of the cell. By adjusting the position of the light source, the detection light emitted by the light source is incident on the SPR sensor, so that the projection position of the detection light is satisfied, and two beams of SPR reflection light can be formed when the detection light is reflected from the SPR sensor. The first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor.
In a specific embodiment of the present invention, the method includes a step-by-step detection method, which specifically includes: the first step, when no single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a second SPR reflected light ray reflected by the surface of the SPR sensor; and secondly, when the single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a first SPR reflected light ray reflected by the inclined plane of the SPR sensor. And performing spectral analysis according to the received first SPR reflected light signal and the second SPR reflected light signal to acquire the analysis information of the single cell.
In another embodiment of the present invention, the method for synchronous detection specifically includes: when the cell to be detected is placed on the cell to be detected position of the SPR sensor, the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor, and the second receiving device simultaneously receives the second SPR reflected light reflected by the surface of the SPR sensor. And performing spectral analysis according to the received first SPR reflected light signal and the second SPR reflected light signal to acquire the analysis information of the single cell.
Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.
Claims (10)
1. An SPR sensor is characterized by comprising a metal film, wherein a second surface of the metal film is provided with a cell to-be-detected position for placing a cell to be detected, an inclined surface is arranged on a position, opposite to the cell to-be-detected position, of a first surface of the metal film opposite to the second surface, and the inclined surface has a second slope different from a first slope of the first surface.
2. The SPR sensor of claim 1 wherein said first slope and said second slope are both such that total reflection occurs when incident light impinges on said first surface.
3. The SPR sensor of claim 1, wherein the size of the spot to be mapped to the size of a single cell.
4. The SPR sensor of claim 1, wherein the area of the inclined plane projected onto the second surface matches the area of the spot to be probed of the cell.
5. A single cell detection device based on SPR, comprising the SPR sensor of any one of claims 1 to 4, a light source, fiber optical tweezers, a microscope and a first receiving means; the optical fiber tweezers are used for placing a single cell to be detected in a cell to-be-detected position of the SPR sensor;
the microscope is used for controlling the fiber optical tweezers to move the single cell to be detected under the observation of the microscope;
the light source is used for projecting detection light rays onto the SPR sensor, and the projection position of the detection light rays meets the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor;
the first receiving device is used for receiving the first SPR reflected light reflected by the inclined plane of the SPR sensor.
6. The SPR-based single cell detection apparatus according to claim 5, wherein said first receiving means is further adapted to receive the second SPR light reflected from the surface of said SPR sensor when no single cell to be detected is located on the spot to be detected of the cell of said SPR sensor.
7. The SPR-based single cell detection apparatus of claim 5, wherein said detection apparatus further comprises a second receiving means, said first receiving means is adapted to receive a first SPR reflected light beam reflected from said inclined surface of said SPR sensor when said cell to be detected is placed on a cell spot to be detected of said SPR sensor, said second receiving means is adapted to simultaneously receive a second SPR reflected light beam reflected from said surface of said SPR sensor.
8. A method of detection of a single cell SPR-based detection apparatus according to any one of claims 5 to 7, said method comprising:
placing the single cell to be detected on the cell to be detected position of the SPR sensor under a microscope through optical fiber tweezers;
projecting detection light rays onto the SPR sensor, wherein the projection positions of the detection light rays meet the requirement that two SPR reflection light rays can be formed when the detection light rays are reflected from the SPR sensor;
the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor.
9. The SPR-based single cell detection method according to claim 8, wherein said method comprises a step-by-step detection method, in particular comprising:
the first step, when no single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a second SPR reflected light ray reflected by the surface of the SPR sensor;
and secondly, when the single cell to be detected is placed on the position to be detected of the cell, the first receiving device receives a first SPR reflected light ray reflected by the inclined plane of the SPR sensor.
10. The SPR-based single cell detection method according to claim 8, wherein said method of simultaneous detection specifically comprises:
when the cell to be detected is placed on the cell to be detected position of the SPR sensor, the first receiving device receives the first SPR reflected light reflected by the inclined plane of the SPR sensor, and the second receiving device simultaneously receives the second SPR reflected light reflected by the surface of the SPR sensor.
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| JPH02145943A (en) * | 1988-11-28 | 1990-06-05 | Fuji Electric Co Ltd | Reflection measurement for limited fine point part |
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| CN103604777A (en) * | 2013-12-02 | 2014-02-26 | 暨南大学 | Orthogonal polarization optical fiber biological refractive index sensor and detecting method thereof |
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| CN105717071A (en) * | 2016-02-19 | 2016-06-29 | 清华大学 | Surface plasma resonance sensing chip and cell response detection system and method |
-
2020
- 2020-10-22 CN CN202011140068.1A patent/CN112285064B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH02145943A (en) * | 1988-11-28 | 1990-06-05 | Fuji Electric Co Ltd | Reflection measurement for limited fine point part |
| WO2013122072A1 (en) * | 2012-02-13 | 2013-08-22 | 国立大学法人東京医科歯科大学 | Method and device for measuring blood information |
| CN103604777A (en) * | 2013-12-02 | 2014-02-26 | 暨南大学 | Orthogonal polarization optical fiber biological refractive index sensor and detecting method thereof |
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| Title |
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| OSCAR FERNANDO D’URSO: "Listeria monocytogenes detection with Surface plasmon", 《IEEE》 * |
| TAO LI: "Power-Referenced Optical Fiber Refractometer", 《IEEE》 * |
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