CN112280668A - Citric acid fermentation system and fermentation method thereof - Google Patents
Citric acid fermentation system and fermentation method thereof Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 261
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 title claims abstract description 87
- 238000000034 method Methods 0.000 title claims abstract description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 36
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- 238000007599 discharging Methods 0.000 claims description 9
- 238000010564 aerobic fermentation Methods 0.000 claims description 6
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
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- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
- B08B9/0813—Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
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Abstract
The invention provides a citric acid fermentation system and a fermentation method thereof, wherein the citric acid fermentation system comprises: fermenting installation and control system, control system with fermenting installation connects and controls fermenting installation's operating condition in order to realize, fermenting installation is by the parallelly connected formation of one-level fermentation cylinder and second grade fermentation cylinder, the one-level fermentation cylinder and the top of second grade fermentation cylinder all is provided with the entering of fungus liquid import and feed liquid import in order to be used for fungus liquid and feed liquid, the bottom of one-level fermentation cylinder and second grade fermentation cylinder is provided with the liquid outlet in order to be used for the fermentation product to discharge. The citric acid fermentation system solves the problem that air and fermentation liquor cannot be fully mixed in a fermentation tank in the prior art.
Description
Technical Field
The invention relates to the field of fermentation, and particularly relates to a citric acid fermentation system and a fermentation method thereof.
Background
Industrial fermentation is an industrial process for converting fermentation raw materials into microbial products required by human beings through the vital activities of microorganisms. In our country, the fermentation industry has been developed greatly in recent years as an important branch in biotechnology, and new fermentation industries (e.g., amino acids, enzyme preparations, organic acids, single-cell proteins, starch sugars, etc.) have been increasing at an average rate of 21% per year. To date, China has formed a fermentation industrial system with various varieties, complete categories and a considerable scale, and the product application covers various industries such as medicine, sanitation, light industry, agriculture, energy, environmental protection and the like.
In aerobic fermentation, the dissolved oxygen content is a limiting factor of thallus growth and fermentation, and in the traditional aerobic fermentation process, the dissolved oxygen concentration in the fermentation liquor is generally only 7-8 ppm, so that the fermentation efficiency is low. Therefore, it is an important direction for the development of modern fermentation industry to seek a fermentation apparatus with higher gas-liquid mass transfer efficiency to increase the dissolved oxygen concentration and improve the fermentation yield.
In addition, the existing fermentation process requires a lot of manpower, including access to the inside of the tank through a manhole for washing, and on-site operation of valves of various pipes. Therefore, the labor cost is improved, and the production safety is reduced.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The first purpose of the invention is to provide a citric acid fermentation system, which is characterized in that a micro-interface generator and a liquid ejector are arranged in a fermentation device, on one hand, the liquid ejector can efficiently crush water for cleaning a tank body into micron-sized liquid drops, and the micron-sized liquid drops are collided above a fermentation tank through the ejector to replace manual cleaning, on the other hand, air is efficiently crushed into micron-sized bubbles through the micro-interface generator and dispersed into fermentation liquor to form a micro-interface system, so that the gas-liquid phase interface area in reverse gas-liquid is increased by tens of times, the mass transfer rate of oxygen to the fermentation liquor is greatly increased, the concentration of dissolved oxygen and the macroscopic fermentation rate are increased, furthermore, the intelligent cleaning device is adopted to replace manual cleaning, a PLC (or DCS, PLC and DCS) control system is adopted to remotely control production, and the production is in line with the production.
The second purpose of the invention is to provide a fermentation method by adopting the citric acid fermentation system, the fermentation method is simple and convenient to operate, the obtained fermentation product has high purity and good fermentation effect, and the method is worthy of wide popularization and application.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
the invention provides a citric acid fermentation system, which comprises: the fermentation device is formed by connecting a primary fermentation tank and a secondary fermentation tank in parallel, the tops of the primary fermentation tank and the secondary fermentation tank are provided with a bacterial liquid inlet and a feed liquid inlet for the entry of bacterial liquid and feed liquid, and the bottoms of the primary fermentation tank and the secondary fermentation tank are provided with liquid outlets for discharging fermentation products;
the fermentation tank comprises a primary fermentation tank, a secondary fermentation tank and a liquid ejector, wherein the primary fermentation tank and the secondary fermentation tank are internally provided with a plurality of liquid ejectors and micro-interface generators, the side wall of the fermentation tank is provided with a cleaning water inlet and an air inlet, the air inlet is communicated into the micro-interface generators through a pipeline, the air inlet corresponds to the micro-interface generators one by one, the liquid ejectors are connected with the cleaning water inlet through pipelines, and the cleaning water inlet corresponds to the liquid ejectors one by one.
In the citric acid fermentation system in the prior art, the fermentation yield is not high generally due to low gas-liquid mass transfer efficiency in the aerobic fermentation process, and the invention provides a novel citric acid fermentation system for solving the technical problems.
The fermentation device is formed by connecting the primary fermentation tank and the secondary fermentation tank in parallel, and the fermentation effect can be improved practically by the parallel operation mode of the two fermentation tanks in order to improve the fermentation effect and the mass transfer effect in the fermentation process. Meanwhile, the working mode can also improve the fermentation treatment capacity and shorten the treatment time.
Preferably, the number of the micro-interface generators in the primary fermentation tank is two, the number of the liquid ejectors is three, the micro-interface generator positioned at the upper part is close to the top of the fermentation tank, the micro-interface generator positioned at the lower part is close to the bottom of the fermentation tank, the liquid ejectors are both arranged at the upper part in the fermentation tank, two of the liquid ejectors are respectively arranged on the side wall of the fermentation tank, and the other liquid ejector is arranged opposite to the micro-interface generator at the upper part.
Preferably, the number of the micro-interface generators in the secondary fermentation tank is three, the number of the liquid ejectors is one, the micro-interface generators are sequentially arranged from top to bottom along the vertical direction, the micro-interface generator positioned on the upper portion is close to the top of the fermentation tank, the micro-interface generator positioned on the lower portion is close to the bottom of the fermentation tank, and the liquid ejectors are all arranged on the upper portion in the fermentation tank.
Preferably, the two liquid jets arranged on the side walls of each fermenter are vertically offset from one another.
Preferably, the liquid ejector is located directly above the micro-interface generator in the upper part.
Preferably, the top of the liquid ejector is a semicircular arc surface, a plurality of injection ports are sequentially arranged on the semicircular arc surface, and the cleaning water inlet is connected with the bottom of the liquid ejector through a pipeline.
The liquid ejector is mainly connected with a cleaning water inlet through a pipeline, cleaning water is introduced into the liquid ejector and is ejected through the ejection openings on the semicircular arc surface of the liquid ejector, the semicircular arc surface structure is designed to improve the cleaning effect and ensure that the ejected cleaning water can carry out all-around cleaning on the wall surface of a fermentation tank, and in addition, preferably, a net surface with a plurality of micropores uniformly distributed is paved in each ejection opening, so that the ejected water is broken and dispersed into fog to improve the mass transfer effect and also play a role in achieving the corresponding effect of the micro-interface generator.
In the primary fermentation tank, three liquid sprayers are specifically matched for use, wherein one liquid sprayer is positioned in the middle, and the other two liquid sprayers are positioned on the inner wall of the fermentation tank, so that the direction of spraying water mist by each liquid sprayer is different, and the inside of the fermentation tank is cleaned from various angles. The two liquid ejectors on the inner wall are also positioned in a staggered manner to prevent the two liquid ejectors from interfering with each other.
It should be noted that, two micro-interface generators are preferably arranged at a lower position in the fermentation tank, so that air can enter and fill the whole fermentation tank, if the position is too high, the dispersion effect between oxygen and bacteria liquid and feed liquid can be influenced, and the other micro-interface generator is arranged at a position close to the upper liquid ejector.
In the secondary fermentation tank, the liquid ejectors are single, and the secondary fermentation tank is mainly used for matching with the primary fermentation tank to act, so that the number of the liquid ejectors arranged in the secondary fermentation tank can be properly adjusted, and the secondary fermentation tank does not need to be too many. The micro-interface generator in the secondary fermentation tank is three and is arranged in sequence along the vertical direction, the connecting rods are arranged between the micro-interface generator and the secondary fermentation tank to play a role in strengthening stability, the space in the fermentation tank is large, if only two spaces which can be dispersed and crushed are limited, the good effect cannot be exerted, and each area in the fermentation tank can be just fully utilized through the arrangement of the three micro-interface generators, so that the dispersing and crushing effect is optimal.
Moreover, the micro-interface generators arranged in the primary fermentation tank and the secondary fermentation tank are matched with each other to work, and the specific type of the micro-interface generator is preferably a pneumatic micro-interface generator, because the pneumatic type is relatively low in cost and easy to install. And each micro-interface generator corresponds to an air inlet respectively, so that each micro-interface generator can realize the effect of dispersing and crushing incoming air at the first time, and preferably, two micro-interface generators do not correspond to the same air inlet at the same time, but air is blown into the fermentation tank in a one-to-one correspondence mode.
The micro-interface generator breaks air into micro-bubbles with micron scale, and releases the micro-bubbles into the fermentation liquid, so as to increase the mass transfer area of the phase boundary between oxygen and the fermentation liquid in the fermentation process, ensure that two phases are fully contacted, improve the concentration of dissolved oxygen in the fermentation liquid, improve the fermentation efficiency and shorten the fermentation time.
It will be appreciated by those skilled in the art that the micro-interface generator used in the present invention is described in the prior patents of the present inventor, such as the patents of application numbers CN201610641119.6, CN201610641251.7, CN201710766435.0, CN106187660, CN105903425A, CN109437390A, CN205833127U and CN 207581700U. The detailed structure and operation principle of the micro bubble generator (i.e. micro interface generator) is described in detail in the prior patent CN201610641119.6, which describes that "the micro bubble generator comprises a body and a secondary crushing member, wherein the body is provided with a cavity, the body is provided with an inlet communicated with the cavity, the opposite first end and second end of the cavity are both open, and the cross-sectional area of the cavity decreases from the middle of the cavity to the first end and second end of the cavity; the secondary crushing member is disposed at least one of the first end and the second end of the cavity, a portion of the secondary crushing member is disposed within the cavity, and an annular passage is formed between the secondary crushing member and the through holes open at both ends of the cavity. The micron bubble generator also comprises an air inlet pipe and a liquid inlet pipe. "the specific working principle of the structure disclosed in the application document is as follows: liquid enters the micro-bubble generator tangentially through the liquid inlet pipe, and gas is rotated at a super high speed and cut to break gas bubbles into micro-bubbles at a micron level, so that the mass transfer area between a liquid phase and a gas phase is increased, and the micro-bubble generator in the patent belongs to a pneumatic micro-interface generator.
In addition, the first patent 201610641251.7 describes that the primary bubble breaker has a circulation liquid inlet, a circulation gas inlet and a gas-liquid mixture outlet, and the secondary bubble breaker communicates the feed inlet with the gas-liquid mixture outlet, which indicates that the bubble breakers all need to be mixed with gas and liquid, and in addition, as can be seen from the following drawings, the primary bubble breaker mainly uses the circulation liquid as power, so that the primary bubble breaker belongs to a hydraulic micro-interface generator, and the secondary bubble breaker simultaneously introduces the gas-liquid mixture into an elliptical rotating ball for rotation, thereby realizing bubble breaking in the rotating process, so that the secondary bubble breaker actually belongs to a gas-liquid linkage micro-interface generator. In fact, the micro-interface generator is a specific form of the micro-interface generator, whether it is a hydraulic micro-interface generator or a gas-liquid linkage micro-interface generator, however, the micro-interface generator adopted in the present invention is not limited to the above forms, and the specific structure of the bubble breaker described in the prior patent is only one of the forms that the micro-interface generator of the present invention can adopt.
Furthermore, the prior patent 201710766435.0 states that the principle of the bubble breaker is that high-speed jet flows are used to achieve mutual collision of gases, and also states that the bubble breaker can be used in a micro-interface strengthening reactor to verify the correlation between the bubble breaker and the micro-interface generator; moreover, in the prior patent CN106187660, there is a related description on the specific structure of the bubble breaker, see paragraphs [0031] to [0041] in the specification, and the accompanying drawings, which illustrate the specific working principle of the bubble breaker S-2 in detail, the top of the bubble breaker is a liquid phase inlet, and the side of the bubble breaker is a gas phase inlet, and the liquid phase coming from the top provides the entrainment power, so as to achieve the effect of breaking into ultra-fine bubbles, and in the accompanying drawings, the bubble breaker is also seen to be of a tapered structure, and the diameter of the upper part is larger than that of the lower part, and also for better providing the entrainment power for the liquid phase.
Since the micro-interface generator was just developed in the early stage of the prior patent application, the micro-interface generator was named as a micro-bubble generator (CN201610641119.6), a bubble breaker (201710766435.0) and the like in the early stage, and is named as a micro-interface generator in the later stage along with the continuous technical improvement, and the micro-interface generator in the present invention is equivalent to the micro-bubble generator, the bubble breaker and the like in the prior art, and has different names.
In summary, the micro-interface generator of the present invention belongs to the prior art, although some micro-interface generators belong to the pneumatic type micro-interface generator, some micro-interface generators belong to the hydraulic type micro-interface generator, and some micro-interface generators belong to the gas-liquid linkage type micro-interface generator, the difference between the types is mainly selected according to the different specific working conditions, and the connection between the micro-interface generator and the reactor and other devices, including the connection structure and the connection position, is determined according to the structure of the micro-interface generator, which is not limited.
Preferably, the citric acid fermentation system comprises a liquid strain premixing device and a strain tank, the strain tank is communicated with the strain inlet, and strains premixed by the liquid strain premixing device are stored in the strain tank.
Preferably, the liquid strain premixing device comprises a shunting premixing pipe and a converging premixing pipe, different kinds of bacteria liquid are converged into the converging premixing pipe through the shunting premixing pipe for premixing, and the converging premixing pipe is communicated with the bacteria liquid tank.
The strain premixing device is designed to realize premixing among multiple liquid strains, different types of bacteria liquid firstly pass through different shunting premixing pipes and then are gathered into a converging premixing pipe to be mixed among different bacteria liquids, so that premixing rate of the strains is improved after different bacteria liquids are mixed, and subsequent fermentation effect is also improved.
Preferably, the citric acid fermentation system comprises a feed liquid tank, and the feed liquid tank is communicated with the feed liquid inlet through a pipeline. The feed liquid stored in the feed liquid tank enters the fermentation tank through the feed liquid inlet.
Preferably, the two sides of the bottom of the fermentation tank are flat, a bulge is formed upwards in the center of the bottom of the fermentation tank, and the liquid outlets are respectively arranged in the flat positions on the two sides of the bottom of the fermentation tank. The reason why the center position is protruded upward is to discharge the fermentation product generated by aerobic fermentation from the discharge hole efficiently, and a small amount of the fermentation product remains if the bottom is flat, so it is preferable to perform the protrusion of the center position of the bottom of the fermentation tank upward according to the embodiment of the present invention.
Preferably, a stirring paddle is arranged at the central bulge of the bottom in the fermentation tank to play a role in accelerating discharging. The bellied position sets up the stirring rake also is in order to improve fermentation product exhaust efficiency, can also promote fermentation efficiency through the stirring moreover.
Preferably, the citric acid fermentation system further comprises a plurality of high-temperature steam pipelines for sterilizing the fermentation tank, and the high-temperature steam pipelines are used for respectively sterilizing the feed liquid, the bacterial liquid and the cleaning water.
Preferably, an ultrasonic sterilization device is arranged in the fermentation tank and is tightly attached to the inner wall of the fermentation tank, so that steam can be introduced into the fermentation tank through a steam pipeline and the ultrasonic sterilization device can be used for assisting sterilization.
Preferably, the position close to the bottom of the fermentation tank is provided with the cleaning water inlet so as to clean the bottom in the fermentation tank. Therefore, the effect of cleaning the upper part in the fermentation tank can be achieved, and the effect of cleaning the lower part in the fermentation tank can also be achieved.
In addition, the invention also provides a fermentation method, which comprises the following steps:
crushing the cleaning water into micron-sized micro-droplets to clean the interior of the fermentation device;
dispersing and crushing the air micro interface, mixing with a bacterial liquid and a feed liquid, carrying out aerobic fermentation, discharging and collecting a fermentation product.
In a word, the fermentation method of the invention is characterized in that a micro-interface generator is arranged in each fermentation tank in the fermentation device, so that before the air and the fermentation liquor are fermented, the micro-interface generator breaks the air into micro bubbles with the diameter of more than or equal to 1 mu m and less than 1mm, the air is contacted with the fermentation liquor in a micro bubble state, the phase boundary mass transfer area between the oxygen and the fermentation liquor in the fermentation process is increased, the two phases are fully contacted, the dissolved oxygen concentration in the fermentation liquor is improved, the fermentation efficiency is improved, the fermentation time is shortened, and the problem of low fermentation efficiency in the prior art is solved.
Especially in this citric acid fermentation system, realized automatic fermentation cylinder cleaning process and intelligent control process through adding control system, further practiced thrift manufacturing cost.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the citric acid fermentation system, the micro-interface generator and the liquid ejector are arranged in the fermentation tank, so that on one hand, the liquid ejector can efficiently crush water in the cleaning tank body into micron-sized liquid drops, and the micron-sized liquid drops are collided above the fermentation tank through the ejector to replace manpower for cleaning, on the other hand, air is efficiently crushed into micron-sized bubbles through the micro-interface generator and dispersed into fermentation liquor to form a micro-interface system, the gas-liquid phase interface area in reverse gas-liquid is increased by tens of times, the mass transfer rate of oxygen to the fermentation liquor is greatly increased, and the concentration of dissolved oxygen and the macroscopic fermentation rate are increased;
(2) according to the citric acid fermentation system, an intelligent cleaning device is adopted to replace manual cleaning, and a PLC (or DCS, PLC and DCS) control system is adopted to remotely control production, so that the production intelligence is met;
(3) the fermentation method is simple and convenient to operate, and the obtained fermentation product has high purity and good fermentation effect, and is worthy of wide popularization and application.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a schematic structural diagram of a citric acid fermentation system provided by an embodiment of the invention;
FIG. 2 is a view showing the internal structure of the primary fermenter shown in FIG. 1;
FIG. 3 is a view showing the internal structure of the secondary fermentation tank of FIG. 1;
fig. 4 is a schematic structural diagram of a mesh surface of a citric acid fermentation system provided in an embodiment of the present invention.
Description of the drawings:
10-primary fermentation tank; 101-a bacterium liquid inlet;
102-feed liquid inlet; 103-a liquid ejector;
104-a micro-interface generator; 105-an air inlet;
106-wash water inlet; 1031-jet orifice;
1032-mesh side; 107-a liquid outlet;
108-a stirring paddle; 109-ultrasonic sterilization device;
110-a fungal solution tank; 111-feed solution tank;
112-liquid spawn premixing device; 1121-shunting the premix tube;
1122-sink premix tube; 20-a control system;
30-second stage fermentation tank.
Detailed Description
The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and the detailed description, but those skilled in the art will understand that the following described embodiments are some, not all, of the embodiments of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In order to more clearly illustrate the technical solution of the present invention, the following description is made in the form of specific embodiments.
Examples
Referring to fig. 1, a citric acid fermentation system according to an embodiment of the present invention mainly includes a fermentation apparatus and a control system 20, the fermentation apparatus is composed of a primary fermentation tank 10 and a secondary fermentation tank 30 connected in parallel, specifically, a structural schematic diagram of the interior of the primary fermentation tank 10 is shown in fig. 2, a structural schematic diagram of the interior of the secondary fermentation tank is shown in fig. 3, three liquid ejectors 103 and two micro-interface generators 104 are disposed in the primary fermentation tank 10, the micro-interface generator 104 located at an upper portion is close to a top portion of the primary fermentation tank 10, the micro-interface generator 104 located at a lower portion is close to a bottom portion of the primary fermentation tank 10, the liquid ejectors 103 are disposed at an upper portion of the primary fermentation tank 10, wherein the two liquid ejectors 103 are respectively disposed on a side wall of the primary fermentation tank 10 and are staggered with each other, the micro-interface generator 104 is arranged right above the micro-interface generator 104, a cleaning water inlet 106 and an air inlet 105 are arranged on the side wall of the primary fermentation tank 10, a bacteria liquid inlet 101 and a material liquid inlet 102 are formed in the top of the primary fermentation tank 10 and used for allowing bacteria liquid and material liquid to enter, the micro-interface generator 104 is connected with the air inlet 105 through a pipeline, the cleaning water inlet 106 corresponds to the liquid ejector 103 in a one-to-one mode and used for dispersing and crushing the cleaning water in the liquid ejector 103, the air inlet 105 corresponds to the micro-interface generator 104 in a one-to-one mode and used for crushing air into micro-bubbles with micron-sized diameters before fermentation reaction, so that the phase boundary mass transfer area between oxygen and fermentation liquor in the fermentation process is increased, two phases are in full contact, the dissolved oxygen concentration in the fermentation.
The number of the micro-interface generators 104 of the secondary fermentation tank 30 is three, the number of the liquid ejectors 103 is one, the micro-interface generators 104 are sequentially arranged from top to bottom along the vertical direction, the micro-interface generator 104 positioned at the upper part is close to the top of the secondary fermentation tank 30, the micro-interface generator 104 positioned at the lower part is close to the bottom of the secondary fermentation tank 30, and the liquid ejectors 103 are all arranged at the upper part in the secondary fermentation tank 30. The liquid injector 103 is located directly above the upper micro-interface generator 104.
This fermentation system includes liquid bacterial species premixing device 112, fungus liquid jar 110 and feed liquid jar 111, and liquid bacterial species premixing device 112 includes reposition of redundant personnel premix tube 1121 and converges and premix tube 1122, and different kinds of fungus liquid converge through reposition of redundant personnel premix tube 1121 and converge and premix in premix tube 1122 and carry out the premix, converge and premix tube 1122 and fungus liquid jar 110 intercommunication, and feed liquid jar 111 passes through the pipeline with feed liquid import 102 and communicates, and fungus liquid jar 110 passes through the pipeline with fungus liquid import 101 and communicates.
The liquid ejector 103 is connected with a cleaning water inlet 106 through a pipeline, the top surface of the liquid ejector 103 is in a semicircular arc surface, a plurality of injection ports 1031 are sequentially arranged on the semicircular arc surface, the cleaning water inlet 106 is connected with the bottom of the liquid ejector 103 through a pipeline, and the cleaning water is crushed into micro-droplets with micron-sized diameters and is sprayed out of the injection ports 1031 protruding from the surface so as to impact the inside of the tank body to carry out efficient cleaning. In order to improve the cleaning effect, a mesh surface 1032 with a plurality of uniformly distributed micropores is laid in the spray opening 1031, the number of the mesh surfaces 1032 is not limited, in order to spray the cleaning water in a mist form and improve the cleaning mass transfer effect, and the structure of the mesh surface 1032 is specifically shown in fig. 4.
The bottoms of the first-stage fermentation tank 10 and the second-stage fermentation tank 30 are provided with liquid outlets 107 for discharging waste water and waste gas and discharging products. The bottom both sides of one-level fermentation cylinder 10 and second grade fermentation cylinder 30 level, and central point puts upwards to form the arch, levels the position in the both sides of one-level fermentation cylinder 10 and second grade fermentation cylinder 30 bottom and is provided with respectively liquid outlet 107, the effect of row material is provided with stirring rake 108 in order to play with higher speed in the protruding department of bottom center in one-level fermentation cylinder 10 and the second grade fermentation cylinder 30, and the direction of blade is up to be used for the stirring when one-level fermentation cylinder 10 and second grade fermentation cylinder 30 bottom are washed, are fermented and are discharged liquid, the rotational speed can.
In order to cooperate with the cleaning, a cleaning water inlet 106 is correspondingly arranged at the bottom of the side wall of the primary fermentation tank 10 and the secondary fermentation tank 30. This enables efficient cleaning of the inside of the primary fermentation tank 10 and the secondary fermentation tank 30 at various positions.
The primary fermentation tank 10 and the secondary fermentation tank 30 of the invention can also realize the disinfection function, each feeding pipeline is correspondingly provided with a steam pipeline, and the inner side wall surfaces of the primary fermentation tank 10 and the secondary fermentation tank 30 are correspondingly provided with an ultrasonic sterilization device 109 to assist the steam pipeline in sterilization.
This embodiment also includes a PLC (or DCS, PLC and DCS) control system 20: and the sensors are connected with the sensors of the first-stage fermentation tank 10 and the second-stage fermentation tank 30 and are used for intelligently controlling the process operation and parameters, so that the remote control of production is realized, and the production intelligence is met.
The specific fermentation process of the erythromycin of the embodiment of the invention is as follows:
(1) cleaning: the cleaning water above the first-stage fermentation tank 10 and the second-stage fermentation tank 30 is conveyed to the inside of the liquid sprayer 103, sprayed out through the spray opening 1031 on the arc surface, efficiently crushed into micron-sized (d is more than or equal to 1 mu m and less than 1mm) liquid drops through the mesh surface 1032, sprayed out through the spray opening 1031 protruding from the surface of the liquid sprayer 103, collided with the inside of the first-stage fermentation tank 10 and the second-stage fermentation tank 30, and cleaned above. The liquid drops after cleaning form a liquid level at the bottom of the tank, when the liquid level rises to the middle part of the tank body, the upper water conveying valve is closed, and the stirring paddle 108 at the bottom is opened to 200 rpm. Because the installation direction of the blade of the stirring paddle 108 is downward, the liquid above the blade is pumped to the two sides below, and vortexes are formed on the two sides of the stirring paddle 108, so that the two sides of the lower parts of the first-stage fermentation tank 10 and the second-stage fermentation tank 30 are washed and cleaned. And simultaneously, a water channel at the lower part is opened, so that water flow enters from a cleaning water inlet 106 at the lower part, the input water flow cleans the wall of the high-side tank under the action of a stirring paddle 108, a water conveying valve is closed after the water flow is washed for 30min, the rotating speed is reduced to 100rpm/min, waste water is discharged through liquid outlets 107 at two sides of the lower end of the tank body, the liquid outlet 107 is closed, and the rotating speed is reduced to 50 rpm.
(2) And (3) disinfection: opening a high-temperature steam pipeline, introducing 121-180 ℃, sterilizing and disinfecting by 3 kg of steam, and maintaining the stirring speed at 50rpm while introducing the steam so that the steam in the tank body is uniformly distributed. The ultrasonic sterilization device 109 on the inner wall of the upper side of the tank body can be opened to assist sterilization at the same time of steam sterilization. And (3) closing the steam pipeline after 30-50 min, discharging water condensed by the steam from liquid outlets 107 at two sides of the bottom of the tank, closing the liquid outlets 107, and cooling the tank body to room temperature.
(3) Feeding: and opening the feed liquid inlets 102 at the tops of the first-stage fermentation tank 10 and the second-stage fermentation tank 30, feeding the erythromycin from the feed liquid inlets 102 through pipelines, and closing the valves to stop feeding when the liquid level reaches a certain height and the feeding is finished.
(4) And (3) disinfection: opening a high-temperature steam pipeline, introducing 121-180 ℃, sterilizing and disinfecting the pipeline passing through the material liquid and the material liquid by 3 kg of steam respectively, maintaining the rotating speed of the stirring paddle 108 at 50rpm, closing a steam valve after 30-50 min, and cooling the tank body to room temperature.
(5) Ventilating: after the feed liquid is cooled to room temperature, fermented fungi are introduced from the bacteria liquid inlet 101, the stirring speed is increased to 100rpm, the bacteria inlet pipeline is closed, and air is introduced into the micro-interface generator 104 from the air inlet 105. The micro-interface generator 104 breaks the air into micro-bubbles with micron scale, and releases the micro-bubbles into the fermentation liquid, so as to increase the mass transfer area of the phase boundary between the oxygen and the fermentation liquid in the fermentation process, make the two phases fully contacted, improve the concentration of dissolved oxygen in the fermentation liquid, improve the fermentation efficiency, and shorten the fermentation time.
(6) Discharging: after the fermentation is completed, stopping ventilation, opening the liquid outlet 107 to discharge materials, reducing the stirring speed to 50rpm, after the materials are completely discharged, the material liquid is used for subsequent separation and purification, the waste gas in the fermentation tank 10 is replaced by air after being stirred for a period of time, and the discharge valve is closed to perform the next circular fermentation process.
The operation and technological parameters in the fermentation process are completely controlled by a control system 20 of a PLC (or DCS, PLC and DCS), and the control system 20 is connected with sensors on the primary fermentation tank 10 and the secondary fermentation tank 30, so that the automatic intelligent control is realized, and the labor cost is saved.
In the above embodiment, the number of the micro-interface generators 104 is plural, the pressure energy of the gas and/or the kinetic energy of the liquid are converted into the surface energy of the bubbles and transferred to the bubbles, so that the bubbles are broken into micro-bubbles with a diameter of 1 μm or more and a diameter of 1mm or less, and the micro-bubbles are divided into the pneumatic micro-interface generator 104, the hydraulic micro-interface generator 104 and the gas-liquid linkage micro-interface generator 104 according to an energy input mode or a gas-liquid ratio, wherein the pneumatic micro-interface generator 104 is driven by the gas, and the input gas amount is far larger than the liquid amount; the hydraulic micro-interface generator 104 is driven by liquid, and the input gas amount is generally smaller than the liquid amount; the gas-liquid linkage type micro-interface generator 104 is driven by gas and liquid at the same time, and the input gas quantity is close to the liquid quantity. The micro-interface generator 104 is selected from one or more of a pneumatic micro-interface generator 104, a hydraulic micro-interface generator 104 and a gas-liquid linkage micro-interface generator 104.
In order to increase the dispersion and mass transfer effects, an additional micro-interface generator 104 can be additionally arranged, the installation position is not limited in fact, the micro-interface generator can be arranged externally or internally, and the micro-interface generator can be arranged on the side wall in the kettle in a relative mode when the micro-interface generator is arranged internally, so that micro-bubbles discharged from the outlet of the micro-interface generator 104 are opposite.
In the above embodiment, the number of the pump bodies is not specifically required, and the pump bodies may be arranged at corresponding positions as required.
In the above embodiment, the fermentation temperature may be normal temperature or temperature required by the strain, the fermentation pressure is normal pressure, and the fermented substance may be erythromycin, penicillin, etc. besides citric acid.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A citric acid fermentation system, comprising: the fermentation device is formed by connecting a primary fermentation tank and a secondary fermentation tank in parallel, the tops of the primary fermentation tank and the secondary fermentation tank are provided with a bacterial liquid inlet and a feed liquid inlet for the entry of bacterial liquid and feed liquid, and the bottoms of the primary fermentation tank and the secondary fermentation tank are provided with liquid outlets for discharging fermentation products;
the fermentation tank comprises a primary fermentation tank and a secondary fermentation tank, wherein the primary fermentation tank and the secondary fermentation tank are internally provided with a plurality of liquid ejectors and micro-interface generators, the side walls of the primary fermentation tank and the secondary fermentation tank are provided with cleaning water inlets and air inlets, the air inlets are communicated into the micro-interface generators through pipelines, the air inlets are in one-to-one correspondence with the micro-interface generators, the liquid ejectors are connected with the cleaning water inlets through pipelines, and the cleaning water inlets are in one-to-one correspondence with the liquid ejectors.
2. The fermentation system of claim 1, wherein the number of the micro-interface generators in the primary fermentation tank is two, the number of the liquid ejectors is three, the micro-interface generator located at the upper part is close to the top of the fermentation tank, the micro-interface generator located at the lower part is close to the bottom of the primary fermentation tank, and the liquid ejectors are both arranged at the upper part in the primary fermentation tank, wherein two liquid ejectors are respectively arranged on the side wall of the primary fermentation tank, and the other liquid ejector is arranged opposite to the micro-interface generator at the upper part.
3. The fermentation system of claim 1, wherein the number of the micro-interface generators in the secondary fermentation tank is three, the number of the liquid ejectors is one, the micro-interface generators are sequentially arranged from top to bottom along the vertical direction, the micro-interface generator positioned at the upper part is close to the top of the fermentation tank, the micro-interface generator positioned at the lower part is close to the bottom of the secondary fermentation tank, and the liquid ejectors are arranged at the upper part in the secondary fermentation tank.
4. The fermentation system of claim 2, wherein the two liquid injectors disposed on the side wall of the primary fermenter are vertically offset from each other.
5. The citric acid fermentation system of claim 2 or 3, wherein the liquid injector is located directly above the upper micro-interface generator.
6. The citric acid fermentation system of claim 1, wherein the citric acid fermentation system comprises a liquid strain premixing device and a strain tank, the strain tank is communicated with the strain inlet, and strains premixed by the liquid strain premixing device are stored in the strain tank.
7. The citric acid fermentation system of claim 6, wherein the liquid strain premixing device comprises a shunting premixing pipe and a converging premixing pipe, different kinds of bacteria liquid are converged into the converging premixing pipe through the shunting premixing pipe for premixing, and the converging premixing pipe is communicated with the bacteria liquid tank.
8. The citric acid fermentation system of claim 1, wherein the top of the liquid ejector is a semicircular arc surface, a plurality of injection ports are sequentially arranged on the semicircular arc surface, and the cleaning water inlet is connected with the bottom of the liquid ejector through a pipeline.
9. The citric acid fermentation system of any one of claims 1-8, wherein the bottom of each fermentation tank is flat on two sides, a central position of the fermentation tank is convex upward, and the liquid outlets are respectively arranged on the flat positions on two sides of the bottom of the fermentation tank.
10. A fermentation method using the citric acid fermentation system according to any one of claims 1 to 9, comprising the steps of:
crushing the cleaning water into micron-sized micro-droplets to clean the interior of the fermentation device;
dispersing and crushing the air micro interface, mixing with a bacterial liquid and a feed liquid, carrying out aerobic fermentation, discharging and collecting a fermentation product.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011128559.4A CN112280668A (en) | 2020-10-21 | 2020-10-21 | Citric acid fermentation system and fermentation method thereof |
| PCT/CN2020/122872 WO2022082617A1 (en) | 2020-10-21 | 2020-10-22 | Citric acid fermentation system and fermentation method therefor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011128559.4A CN112280668A (en) | 2020-10-21 | 2020-10-21 | Citric acid fermentation system and fermentation method thereof |
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| CN113684115A (en) * | 2021-08-31 | 2021-11-23 | 南京延长反应技术研究院有限公司 | Micro-interface mass transfer enhanced fermentation system and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2543307C3 (en) * | 1975-09-27 | 1981-06-19 | Standard-Messo Duisburg, Gesellschaft für Chemietechnik mbH & Co, 4100 Duisburg | Method and device for fermenting carbohydrate-containing nutrient substrate |
| CN201127917Y (en) * | 2007-11-30 | 2008-10-08 | 马庚 | Universal sprayer |
| CN202131308U (en) * | 2011-07-01 | 2012-02-01 | 南阳启伟微生态基因科技开发有限公司 | Micro-nano bubble generation device and fermentation device using same |
| CN103695319B (en) * | 2013-12-23 | 2016-02-17 | 安徽丰原发酵技术工程研究有限公司 | A kind ofly produce the bacterial strain of citric acid and the method for preparation of citric acid by fermentation thereof |
| CN203923188U (en) * | 2014-06-30 | 2014-11-05 | 孙百虎 | Aerobic fermentation tank |
| CN104293647B (en) * | 2014-09-19 | 2017-09-12 | 利晟生物科技（北京）有限公司 | For the fermentation equipment and zymotechnique continuously fermented or gap is fermented |
| CN206854309U (en) * | 2017-06-29 | 2018-01-09 | 光明乳业股份有限公司 | A kind of CIP purging systems and fermentation tank |
| CN108624480A (en) * | 2018-06-27 | 2018-10-09 | 津药新瑞制药股份有限公司 | A kind of material distribution device being used for two groups of aerobic fermentation tanks |
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2020
- 2020-10-21 CN CN202011128559.4A patent/CN112280668A/en not_active Withdrawn
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| CN113684115A (en) * | 2021-08-31 | 2021-11-23 | 南京延长反应技术研究院有限公司 | Micro-interface mass transfer enhanced fermentation system and method |
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