CN112280650A

CN112280650A - Storage device for detecting drug resistance of bacteria

Info

Publication number: CN112280650A
Application number: CN202011127364.8A
Authority: CN
Inventors: 杜纪英; 曹便利; 马晓娟; 原增艳; 单会东
Original assignee: Sanquan College of Xinxiang Medical University
Current assignee: Sanquan College of Xinxiang Medical University
Priority date: 2020-10-20
Filing date: 2020-10-20
Publication date: 2021-01-29
Anticipated expiration: 2040-10-20
Also published as: CN112280650B

Abstract

The invention discloses a storage device for detecting bacterial drug resistance, which comprises a storage body, wherein a first fixing plate and a second fixing plate are fixedly connected inside the storage body, a placing opening is formed in the side surface of the storage body, a culture dish is placed at the top of the first fixing plate, a water guide pipe is fixedly connected inside the storage body, and an electric telescopic rod is fixedly connected to the side surface of the storage body. According to the invention, the temperature in the storage body can be controlled through expansion with heat and contraction with cold, so that the survival rate of bacteria can be improved, the detection of drug resistance of the bacteria is facilitated, the survival factors of the bacteria are eliminated, the accuracy of the detection of the drug resistance of the bacteria can be improved, a proper environment is provided for the research of the drug resistance of the bacteria, and when the drug resistance of the bacteria is detected, the contact time of a culture dish and air in the detection process is reduced by controlling the opening and closing of the culture dish, so that the phenomenon that the bacteria in the culture dish are polluted can be effectively avoided.

Description

Storage device for detecting drug resistance of bacteria

Technical Field

The invention relates to the technical field of bacteria storage, in particular to a storage device for detecting bacterial drug resistance.

Background

The bacteria refer to one of the main groups of organisms, belong to the bacterial domain, are the most abundant group in all organisms, have quite various shapes, mainly have a spherical shape, a rod shape and a spiral shape, are pathogens of a plurality of diseases, can spread the diseases among normal human bodies in various ways, have strong infectivity and have great harm to the society.

At present, when bacteria are detected, the drug resistance of the bacteria is often detected, and the drug resistance of the bacteria can be judged, however, when the bacteria are detected, the pollution of the bacteria in the air is often ignored, the detection result is not accurate, and meanwhile, when the bacteria are stored, the problem of low survival rate can occur, so that the storage device for detecting the drug resistance of the bacteria is provided.

Disclosure of Invention

The invention aims to solve the defect of low storage survival rate in the prior art, and provides a storage device for detecting bacterial drug resistance.

In order to achieve the purpose, the invention adopts the following technical scheme:

a storage device for detecting drug resistance of bacteria comprises a storage body, wherein a first fixing plate and a second fixing plate are fixedly connected inside the storage body, a placing opening is formed in the side face of the storage body, a culture dish is placed at the top of the first fixing plate, a water guide pipe is fixedly connected inside the storage body, an electric telescopic rod is fixedly connected to the side face of the storage body, a first cavity is connected to the top end of the storage body in a sliding mode, a second cavity is fixedly connected to the bottom of the first cavity, the inside of the second cavity is divided into an upper cavity and a lower cavity through a partition plate, a sliding block is connected between the upper cavity and the lower cavity in a sliding mode and is connected with the upper cavity through a second spring, a first connecting rod is rotatably connected to the sliding block through a rotating shaft and is located on the outer side of the second cavity, and a second connecting rod is rotatably connected to the first connecting rod through a rotating, the side fixedly connected with gag lever post of second cavity, the gag lever post rotates through the axis of rotation and is connected with the gear, the one end that first connecting rod was kept away from to the second connecting rod is connected with the gear rotation, the one end fixedly connected with second permanent magnet of gear, the top sliding connection of second fixed plate has first permanent magnet, the first spring of one end fixedly connected with of second permanent magnet is kept away from to first permanent magnet, fixed connection between the one end that first permanent magnet was kept away from to first spring and the inside wall of storage body, the internal connection of storage body has fixture and cooling mechanism.

Preferably, fixture include with electric telescopic handle between fixed connection's the pipeline, sliding connection has two slides on the pipeline, the top of slide is rotated through the axis of rotation and is connected with the third connecting rod, the bottom of slide is rotated through the axis of rotation and is connected with the fourth connecting rod, electric telescopic handle's the spacing frame of side fixedly connected with, the inside sliding connection of spacing frame has two limiting plates, two one side difference fixedly connected with spacing clamp that the limiting plate is close to each other.

Preferably, cooling mechanism includes the loading case with the side fixed connection of memory bank, the one end of aqueduct runs through the memory bank and extends to the inside of loading the case, the fixed pipe of inside fixedly connected with of memory bank, the inside sliding connection of fixed pipe has the piston, the one end of fixed pipe runs through the memory bank and extends to the inside of loading the case.

Preferably, the inside fixedly connected with rack of memory bank, the meshing is connected between rack and the gear, the fixed push pedal that has cup jointed on the second cavity, the push pedal slides on the inside wall of memory bank.

Preferably, two the top fixed connection of slide is passed through axis of rotation and spacing frame to the one end that the slide was kept away from to the third connecting rod, the one end that the slide was kept away from to the fourth connecting rod is passed through the axis of rotation and is connected with the limiting plate rotation, spout sliding connection has the ejector pad through the inside of pipeline, two gleitbretters of fixedly connected with on the ejector pad, the gleitbretter slides on the pipeline, fixed connection between gleitbretter and the slide.

Preferably, a microscope is fixedly connected inside the storage body, the microscope is positioned right in front of the culture dish, and the slide block is provided with a plurality of circulation holes.

Compared with the prior art, the invention has the beneficial effects that:

1. in the invention, the temperature in the storage body can be controlled by expansion with heat and contraction with cold, so that the survival rate of bacteria can be improved, the detection of drug resistance of the bacteria is convenient, the survival factors of the bacteria are eliminated, the accuracy of the detection of the drug resistance of the bacteria can be improved, a suitable environment is provided for the research of the drug resistance of the bacteria, the difficulty of experimental operators is reduced, the problem that the temperature in the storage body cannot be accurately controlled in the prior art is solved, the piston can drive cooling liquid to cool the storage body under the action of pressure according to the temperature in the storage body, so that the problem of low survival rate of the bacteria can be effectively avoided, meanwhile, when the drug resistance of the bacteria is detected, the contact time of a culture dish and air in the detection process can be reduced by controlling the opening and closing of the culture dish, so that the phenomenon of bacterial pollution in the culture dish can be, can ensure going on smoothly of experiment, when controlling the culture dish closure, control the detection liquid simultaneously, can further avoid during the outside air gets into the culture dish, also can control the volume of dropping liquid to can be convenient detect the bacterial drug resistance.

2. Can fix the culture dish through fixture to can avoid the unrestrained of bacterial liquid, and then further avoid the memory bank to receive the pollution and the culture dish in the bacterium impaired, meanwhile, at the in-process of centre gripping, can further promote the flow of condensate water in the pipeline, can strengthen the effect of the internal cooling of memory.

Drawings

FIG. 1 is a schematic structural diagram of a storage device for detecting bacterial drug resistance according to the present invention;

FIG. 2 is a schematic view of a state structure of a storage device for detecting bacterial drug resistance according to the present invention;

FIG. 3 is an enlarged view taken at A in FIG. 1;

FIG. 4 is an enlarged view at B in FIG. 2;

FIG. 5 is a left side view of the gear mesh of FIG. 1;

FIG. 6 is a top view of the clamping mechanism of FIG. 1;

fig. 7 is a left side view of the duct of fig. 6.

In the figure: 1 storage body, 2 first fixed plate, 3 second fixed plate, 4 microscope, 5 first spring, 6 first cavity, 7 push plate, 8 second cavity, 9 first permanent magnet, 10 electric telescopic rod, 11 spacing frame, 12 spacing plate, 13 spacing clip, 14 loading box, 15 aqueduct, 16 fixed tube, 17 piston, 18 slider, 19 second spring, 20 baffle, 21 circulation hole, 22 first connecting rod, 23 second connecting rod, 24 spacing rod, 25 rack, 26 gear, 27 second permanent magnet, 28 pipeline, 29 slide plate, 30 third connecting rod, 31 fourth connecting rod, 32 push block, 33 slide plate, 34 culture dish.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Referring to fig. 1-7, a storage device for detecting bacterial drug resistance, comprising a storage body 1, a first fixing plate 2 and a second fixing plate 3 are fixedly connected inside the storage body 1, a placing opening is formed in a side surface of the storage body 1, the placing opening is located on a back surface of the storage body 1, a culture dish 34 is placed on a top portion of the first fixing plate 2, the culture dish 34 is placed inside the storage body 1 through the placing opening, bacteria liquid is contained inside the culture dish 34, a water guide pipe 15 is fixedly connected inside the storage body 1, an electric telescopic rod 10 is fixedly connected on a side surface of the storage body 1, the electric telescopic rod 10 can push a pipeline 28, so that the culture dish 34 is fixed, the culture dish 34 can be effectively prevented from inclining in the storage body 1, detection of bacterial drug resistance is facilitated, a first cavity 6 is slidably connected to a top end of the storage body 1, and a detection liquid can enter an upper cavity of a second cavity 8 through the first cavity, the detection liquid is controlled, the bottom of the first cavity 6 is fixedly connected with a second cavity 8, the inside of the second cavity 8 is divided into an upper cavity and a lower cavity by a partition plate 20, a slide block 18 is connected between the upper cavity and the lower cavity in a sliding manner, the air tightness between the slide block 18 and the second cavity 8 as well as between the slide block 21 is good, the slide block 18 is connected with the upper cavity by a second spring 19, the slide block 18 is rotatably connected with a first connecting rod 22 by a rotating shaft, the first connecting rod 22 is positioned at the outer side of the second cavity 8, the first connecting rod 22 is rotatably connected with a second connecting rod 23 by the rotating shaft, the side surface of the second cavity 8 is fixedly connected with a limiting rod 24, the limiting rod 24 is rotatably connected with a gear 26 by the rotating shaft, one end of the second connecting rod 23 far away from the first connecting rod 22 is rotatably connected with the gear 26, one end of the gear 26 is fixedly connected, one end of the first permanent magnet 9, which is far away from the second permanent magnet 27, is fixedly connected with a first spring 5, one end of the first spring 5, which is far away from the first permanent magnet 9, is fixedly connected with the inner side wall of the storage body 1, the inside of the storage body 1 is fixedly connected with a rack 25, the rack 25 is meshed with a gear 26, a push plate 7 is fixedly sleeved on the second cavity 8, the air tightness between the push plate 7 and the storage body 1 is good, the push plate 7 slides on the inner side wall of the storage body 1, the inside of the storage body 1 is fixedly connected with a microscope 4, the microscope 4 can conveniently detect bacteria in a culture dish 34, the microscope 4 is positioned right in front of the culture dish 34, the slide block 18 is provided with a plurality of circulation holes 21, the circulation holes 21 formed in the slide block 18 are hollow, the part below the circulation holes 21 formed in the slide block 18 is solid, the plurality of circulation holes 21 can control a detection liquid to enter the lower circulation cavity from the upper, thereby realize controlling detection liquid, when the bottom of second cavity 8 is close culture dish 34, detect in liquid can get into culture dish 34 through second cavity 8, detect the drug resistance of bacterium, avoid the bacterium liquid to receive the pollution, and can further avoid detecting the outside that the liquid splashes culture dish 34, when second cavity 8 is close culture dish 34, the one end magnetic pole that is close to each other between first permanent magnet 9 and the second permanent magnet 27 is the same, produces the repulsion.

The storage body 1 is internally connected with a clamping mechanism and a cooling mechanism, the clamping mechanism comprises a pipeline 28 fixedly connected with the electric telescopic rod 10, cooling water is filled in the pipeline 28, two pushing blocks 32 in the pipeline 28 do not contain condensed water, two sliding plates 29 are slidably connected on the pipeline 28, the sliding plates 29 are arranged in a U shape and can conveniently drive the pushing blocks 32 to slide, the top of each sliding plate 29 is rotatably connected with a third connecting rod 30 through a rotating shaft, the bottom of each sliding plate 29 is rotatably connected with a fourth connecting rod 31 through a rotating shaft, the side surfaces of the electric telescopic rod 10 are fixedly connected with a limiting frame 11, the pipeline 28 penetrates through the limiting frame 11 and surrounds the bottom of a culture dish 34, two limiting plates 12 are slidably connected inside the limiting frame 11, one sides, close to each other, of the two limiting plates 12 are respectively and fixedly connected with a limiting clamp 13, the limiting clamps 13 clamp the culture dish, the pushing block 32 can further promote the flow of condensed water in the pipeline and enhance the cooling effect in the storage body 1, one end of the two third connecting rods 30, which is far away from the sliding plate 29, is fixedly connected with the top of the limiting frame 11 through a rotating shaft, one end of the fourth connecting rod 31, which is far away from the sliding plate 29, is rotatably connected with the limiting plate 12 through a rotating shaft, the inside of the pipeline 28 is slidably connected with the pushing block 32 through a sliding chute, two sliding sheets 33 are fixedly connected on the pushing block 32, the airtightness between the sliding sheets 33 and the pipeline 28 is good, the sliding sheets 33 slide on the pipeline 28, the sliding sheets 33 are fixedly connected with the sliding plate 29, the cooling mechanism comprises a loading box 14 fixedly connected with the side surface of the storage body 1, the inside of the loading box 14 is filled with condensed fluid, one end of the water guide pipe 15 penetrates through the storage body 1 and extends to the inside of the loading box 14, the inside of, piston 17 can drive the coolant liquid and lower the temperature to the storage body 1 according to the internal temperature of storage under the effect of pressure to can effectively avoid the problem that the bacterial survival rate is low, the one end of fixed pipe 16 runs through storage body 1 and extends to the inside of loading case 14.

The invention can be illustrated by the following operating modes:

firstly, the condensed water is filled in the loading box 14, the cooling water is filled in the pipeline 28, the culture dish 34 filled with the bacteria liquid is placed in the storage body 1 through the placing port, at the moment, the culture dish 34 is positioned under the first permanent magnet 9, then, the button of the electric telescopic rod 10 is pressed, under the pushing of the electric telescopic rod 10, the third connecting rod 30 and the fourth connecting rod 31 start to rotate, so that the sliding plate 29 can be driven to start to slide on the pipeline 28, the limiting plate 12 can be driven to slide on the limiting frame 11, the limiting clamp 13 fixedly connected with the limiting plate 12 and positioned on the side surface of the culture dish 34 starts to clamp the culture dish 34, meanwhile, due to the sliding of the sliding plate 29, the pushing block 32 in the pipeline 28 can be driven to slide, the cooling water in the pipeline 28 can be pushed, and the interior of the storage body 1 is cooled.

Then, when the drug resistance detection is performed on bacteria in the culture dish 34, a drug is added into the first cavity 6 to push the first cavity 6, the second cavity 8 descends along with the descending of the first cavity 6, in the descending process of the second cavity 8, due to the meshing connection between the gear 26 and the rack 25, as the second cavity 8 moves downwards, the gear 26 slides on the rack 25, so that the rotation of the gear 26 can be driven, at the moment, the second permanent magnet 27 starts to change towards the magnetic pole at the lower end along with the rotation of the gear 26, the changed magnetic pole is the same as the magnetic pole at the end, which is close to the first permanent magnet 9, so as to generate a repulsive force, the first permanent magnet 9 can be moved away from the upper part of the culture dish 34, meanwhile, the second connecting rod 23 is driven to rotate when the gear 26 rotates, so that the first connecting rod 22 can be driven to rotate, and the sliding block 18 can be further driven to slide in the second cavity 8 through the rotation of the first connecting rod 22, through the arrangement, at this time, the circulation hole 21 formed on the slider 18 moves to the lower end of the partition plate 21 from the upper end of the partition plate 21 along with the movement of the slider 18, the liquid medicine added into the first cavity 6 enters the lower cavity of the second cavity 8 through the circulation hole, and then drops on the bacteria liquid of the culture dish 34, so that the drug resistance of the bacteria liquid can be detected, and similarly, the first cavity 6 is not pushed any more, the first permanent magnet 9 and the second permanent magnet 27 recover to the original positions under the action of the second spring 19 and the pressure, the culture dish 34 is sealed again by the first permanent magnet 9, because the air tightness between the push plate 7 and the storage body 1 is good, the movement of the piston 17 can be pushed in the moving process of the second cavity 8, and meanwhile, the movement of the piston 17 is pushed according to the change of the internal temperature of the storage body 1 through the principle of thermal expansion and cold contraction, so that the internal temperature of the storage body 1 can be adjusted, the storage of bacteria is facilitated, and the change of the bacteria inside the culture dish 34 can be observed through the microscope 4.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The utility model provides a storage device for bacterial drug resistance detection, includes storage body (1), its characterized in that, the inside fixedly connected with first fixed plate (2) and second fixed plate (3) of storage body (1), the side of storage body (1) has seted up places the mouth, culture dish (34) have been placed at the top of first fixed plate (2), the inside fixedly connected with aqueduct (15) of storage body (1), the side fixedly connected with electric telescopic handle (10) of storage body (1), the top sliding connection of storage body (1) has first cavity (6), the bottom fixedly connected with second cavity (8) of first cavity (6), the inside of second cavity (8) is divided into upper chamber and lower cavity through baffle (20), sliding connection has slider (18) between upper chamber and the lower cavity, slider (18) are connected with the upper chamber through second spring (19), the sliding block (18) is connected with a first connecting rod (22) in a rotating mode through a rotating shaft, the first connecting rod (22) is located on the outer side of the second cavity (8), the first connecting rod (22) is connected with a second connecting rod (23) in a rotating mode through the rotating shaft, a limiting rod (24) is fixedly connected to the side face of the second cavity (8), a gear (26) is connected to the limiting rod (24) in a rotating mode through the rotating shaft, one end, far away from the first connecting rod (22), of the second connecting rod (23) is connected with the gear (26) in a rotating mode, one end of the gear (26) is fixedly connected with a second permanent magnet (27), the top of the second fixing plate (3) is connected with a first permanent magnet (9) in a sliding mode, one end, far away from the second permanent magnet (27), of the first permanent magnet (9) is fixedly connected with a first spring (5), one end, far away from the first permanent magnet (9), of the, the inside of the storage body (1) is connected with a clamping mechanism and a cooling mechanism.

2. The storage device for detecting the drug resistance of bacteria according to claim 1, wherein the clamping mechanism comprises a pipeline (28) fixedly connected with the electric telescopic rod (10), two sliding plates (29) are slidably connected onto the pipeline (28), a third connecting rod (30) is rotatably connected to the top of each sliding plate (29) through a rotating shaft, a fourth connecting rod (31) is rotatably connected to the bottom of each sliding plate (29) through a rotating shaft, a limiting frame (11) is fixedly connected to the side surface of the electric telescopic rod (10), two limiting plates (12) are slidably connected to the inside of each limiting frame (11), and two limiting clamps (13) are respectively fixedly connected to the sides of the two limiting plates (12) close to each other.

3. The storage device for detecting the drug resistance of bacteria according to claim 1, wherein the temperature reduction mechanism comprises a loading box (14) fixedly connected with the side surface of the storage body (1), one end of the water conduit (15) penetrates through the storage body (1) and extends to the inside of the loading box (14), a fixed pipe (16) is fixedly connected to the inside of the storage body (1), a piston (17) is slidably connected to the inside of the fixed pipe (16), and one end of the fixed pipe (16) penetrates through the storage body (1) and extends to the inside of the loading box (14).

4. The storage device for detecting the drug resistance of bacteria according to claim 1, wherein a rack (25) is fixedly connected inside the storage body (1), the rack (25) is engaged with a gear (26), a push plate (7) is fixedly sleeved on the second cavity (8), and the push plate (7) slides on the inner side wall of the storage body (1).

5. The storage device for detecting the drug resistance of bacteria according to claim 2, wherein one ends of the two third connecting rods (30) far away from the sliding plate (29) are fixedly connected with the top of the limiting frame (11) through rotating shafts, one ends of the four fourth connecting rods (31) far away from the sliding plate (29) are rotatably connected with the limiting plate (12) through rotating shafts, the inside of the pipeline (28) is slidably connected with a push block (32) through a sliding groove, the push block (32) is fixedly connected with two sliding pieces (33), the sliding pieces (33) slide on the pipeline (28), and the sliding pieces (33) are fixedly connected with the sliding plate (29).

6. The storage device for detecting the drug resistance of bacteria according to claim 1, wherein a microscope (4) is fixedly connected to the inside of the storage body (1), the microscope (4) is positioned right in front of a culture dish (34), and the slide block (18) is provided with a plurality of flow holes (21).