CN112274227A - Golden hair follicle transplantation method - Google Patents
Golden hair follicle transplantation method Download PDFInfo
- Publication number
- CN112274227A CN112274227A CN202011310718.2A CN202011310718A CN112274227A CN 112274227 A CN112274227 A CN 112274227A CN 202011310718 A CN202011310718 A CN 202011310718A CN 112274227 A CN112274227 A CN 112274227A
- Authority
- CN
- China
- Prior art keywords
- hair follicle
- hair
- transplanting
- follicle
- fine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3468—Trocars; Puncturing needles for implanting or removing devices, e.g. prostheses, implants, seeds, wires
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/32—Surgical cutting instruments
- A61B17/322—Skin grafting apparatus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/32—Surgical cutting instruments
- A61B17/322—Skin grafting apparatus
- A61B2017/3225—Skin grafting apparatus with processing of harvested tissue
Landscapes
- Health & Medical Sciences (AREA)
- Surgery (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medical Informatics (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Pathology (AREA)
- Plastic & Reconstructive Surgery (AREA)
- Transplantation (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a golden hair follicle transplantation method, which comprises the following steps: extracting fine and soft hair follicles from a hair line part of the forehead of a patient by using a hair follicle extractor; extracting healthy hair follicles from the back occiput of the patient using a hair follicle extractor; culturing the fine hair follicle and the healthy hair follicle extracted from the occiput in a culture medium at 0-3 deg.C; transplanting the cultured healthy hair follicles of the occiput into an area to be planted, and connecting the healthy hair follicles with the primary hairs; transplanting the cultured fine and soft hair follicle to the newly shaped hairline position to ensure the naturalness of the hairline. The invention solves the problems of poor hair follicle transplanting effect, unnatural hair joining and easy hair follicle necrosis existing in the prior art.
Description
Technical Field
The invention relates to the field of hair follicle transplantation, in particular to a golden hair follicle transplantation method.
Background
With the rapid progress of society, the pace of life is also continuously accelerated, so that the pressure on people in life and work is greatly increased, and compared with too many people, the problems of alopecia and hair loss occur because of too much life pressure. The normal hair loss is usually the hair in the catagen or the telogen period, the hair in the anagen period is supplemented continuously, the two forms dynamic balance, and the hair in the healthy state can maintain the normal quantity unchanged. However, if the stress of life is too great, the balance is broken by the loss of hair due to the stress of life, resulting in a larger amount of hair being lost than newly grown hair. More and more people face the trouble of alopecia, and heavy psychological and physiological burden is brought to patients, and the most effective treatment means at present is hair transplantation. However, the conventional hair transplantation only transplants hairs from other parts to the target part, and cannot fundamentally solve the problem.
The hair follicle stem cell is one of the outer root sheath bulge of human hair follicles. The hair follicle stem cells belong to adult stem cells and have a capability of exhibiting a remarkable proliferation in vivo under the action of a living internal environment. The research finds that the hair follicle stem cell has the multidirectional differentiation potential, can be differentiated into epidermis, hair follicle and sebaceous gland, and is involved in the process of healing skin wounds. However, the existing hair follicle transplantation technology has the problems that the hairs at the transplanted part are not uniform, the connection is not natural, and the transplanted hair follicle is easy to become necrotic.
Disclosure of Invention
Therefore, the invention provides a method for transplanting the hair follicle to solve the problems of poor hair follicle transplanting effect, unnatural hair joining and easy hair follicle necrosis existing in the prior art.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention discloses a golden hair follicle transplantation method, which comprises the following steps:
extracting fine and soft hair follicles from a hair line part of the forehead of a patient by using a hair follicle extractor;
extracting healthy hair follicles from the back occiput of the patient using a hair follicle extractor;
culturing the fine hair follicle and the healthy hair follicle extracted from the occiput in a culture medium at 0-3 deg.C;
transplanting the cultured healthy hair follicles of the occiput into an area to be planted, and connecting the healthy hair follicles with the primary hairs;
transplanting the cultured fine and soft hair follicle to the newly shaped hairline position to ensure the naturalness of the hairline.
Further, the hair follicle extractor has an extraction caliber of 0.4mm-0.6mm, and the hair follicle extraction process is carried out in a sterile environment.
Furthermore, the hair follicle extractor perforates from the position of the hairline and the back occiput, extracts the hair follicle with hair, and separates the hair from the hair follicle to obtain hair follicle stem cells at different parts.
Furthermore, the fine and soft hair follicle belongs to the hair follicle where the hair with the forehead hairline part shorter than the normal hair follicle diameter is located, and the extracted fine and soft hair follicle stem cells are subjected to proliferation culture in a serum-free culture medium to obtain the serum-free cultured hair follicle stem cells.
Further, the healthy hair follicles extracted from the posterior occiput were cultured by proliferation of stem cells in a serum-free medium to obtain serum-free cultured hair follicle stem cells, and the hair grown from the healthy hair follicles in the posterior occiput was thicker than the hair grown from the delicate hair follicles.
Further, the healthy hair follicles of the occiput are cultured and proliferated, injected and planted on the part to be generated for multiple times, and the injection and planting operation is carried out according to the interval period by adopting dispersive injection.
Furthermore, the fine and soft hair follicles are implanted at the position of the newly molded hairline by injection, so that the fine and soft hair follicles grow at the position of the hairline and are close to the original hairline.
Furthermore, the fine and soft hair follicles can be transplanted to an area needing to be encrypted, and the hair follicles after the thick and strong hair follicles are planted can be replanted, so that higher planting density can be achieved.
The invention has the following advantages:
the invention discloses a golden hair follicle transplanting method, which comprises the steps of extracting fine and soft hair follicles and rear occipital healthy hair follicles at hairline positions through a hair follicle extractor, transplanting the rear occipital healthy hair follicles to a region to be planted after cultivation, connecting the hair follicles with primary hair, transplanting the fine and soft hair follicles to the newly molded hairline positions, ensuring the naturalness of the hairline, and achieving the beautiful effect; avoiding unnatural connection, avoiding nutrition competition between the transplanted healthy hair follicle and the thin and soft hair follicle, and improving the survival rate of the hair follicle.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
Fig. 1 is a flowchart of a method for transplanting a golden hair follicle according to an embodiment of the present invention;
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
The embodiment discloses a golden hair follicle transplantation method, which comprises the following steps:
extracting fine and soft hair follicles from a hair line part of the forehead of a patient by using a hair follicle extractor;
extracting healthy hair follicles from the back occiput of the patient using a hair follicle extractor;
culturing the fine hair follicle and the healthy hair follicle extracted from the occiput in a culture medium at 0-3 deg.C;
transplanting the cultured healthy hair follicles of the occiput into an area to be planted, and connecting the healthy hair follicles with the primary hairs;
transplanting the cultured fine and soft hair follicle to the newly shaped hairline position to ensure the naturalness of the hairline.
"golden hair follicle" refers to a hair with a shorter diameter than a normal hair follicle at the hairline region on the forehead of a patient; the 'golden hair follicle transplantation operation' is to take out the healthy hair follicle tissues of the part through the microsurgery technology, transplant the hair follicle of the part to the new plastic hair line position of the patient or other positions needing to be encrypted according to the requirements of the patient after processing and culturing, the regenerated hair after transplantation can keep the original characteristics, can ensure the natural beauty degree of the new plastic hair line, and ensure that the connection of the newly moulded hair line and the original hair is more natural so as to achieve the effect of more natural and beautiful appearance.
The hair follicle extractor extracts the aperture of 0.4mm-0.6mm, the hair follicle extraction process is carried out in an aseptic environment, the hair follicle extractor punches holes from the position of a hairline and the back occiput, extracts the hair follicle with hair, and separates the hair from the hair follicle to obtain hair follicle stem cells at different parts. The extraction of normal primary hair follicles basically uses an extractor with the extracting caliber of about 0.6-1.2mm to extract hair follicles, while the extraction of the hair follicles of the standard hair follicles only needs to use an extractor with the extracting caliber of 0.4-0.6mm to extract the hair follicles, and also, the caliber of an implanting instrument used in the hair follicle implanting process is relatively reduced, so that the transplantation of the standard hair follicles can achieve better density.
The forehead hairline position of a normal person can be distributed with a certain proportion of fine and soft hair follicles, so that a more natural and attractive effect can be achieved, the hair follicles transplanted by using the traditional hair transplantation technology are all robust healthy hair follicles extracted from the occiput, the forehead hairline position after being planted is relatively stiff and slightly unnatural, and the hairline transplanted by using the 'golden hair follicle technology' is accurately transplanted on the newly-modeled hairline after being transplanted in the traditional way, so that the newly-modeled hairline is infinitely close to the original hairline, and the more natural and attractive effect can be achieved.
The fine and soft hair follicles belong to hair follicles where hairs with forehead hairline parts shorter than normal hair follicles in diameter are located, and the extracted fine and soft hair follicle stem cells are subjected to proliferation culture in a serum-free culture medium to obtain culture serum-free hair follicle stem cells; the healthy hair follicle extracted from the occipital part is cultured by stem cells in a serum-free culture medium in a proliferation manner to obtain serum-free cultured hair follicle stem cells, and the hair grown from the healthy hair follicle of the occipital part is thicker than the hair grown from the delicate hair follicle.
Serum-free culture media for stem cells common in the art comprise the following components: 10-15ng/ml of basic fibroblast growth factor, 0.01-0.1ng/ml of thrombin, 1-3mg/ml of recombinant human insulin, 0.05-0.12mg/ml of human transferrin, 0.1-0.2mg/ml of human albumin, 0.1-0.5 mu g/ml of sodium selenite, 0.5-1.2mM of levocarnitine and 5-10 mu m of danshensu.
The stem cell serum-free medium in this example comprises the following components: 12ng/ml of basic fibroblast growth factor, 0.02ng/ml of thrombin, 2mg/ml of recombinant human insulin, 0.1mg/ml of human transferrin, 0.14mg/ml of human serum albumin, 0.2 mu g/ml of sodium selenite, 1mM of levocarnitine and 8 mu m of danshensu.
After the healthy hair follicles of the back occiput are cultured and proliferated, injecting and planting the healthy hair follicles to a part to be grown for multiple times, adopting dispersive injection, and performing injection planting operation according to an interval period; the thin and soft hair follicle is injected and planted at the position of the newly shaped hairline, so that the thin and soft hair follicle grows at the position of the hairline and is close to the original hairline.
The forehead hairline position of normal person all can have the thin and soft hair follicle of a certain quantity proportion, if use traditional hair transplantation technique directly to link up from primary position, the condition that can appear linking position existence a certain amount of thin and soft hair follicle in later stage growth process exists, because the thickness degree inconsistent of hair follicle, so comparatively obvious linking unnatural area can appear in this region, there is great influence to pleasing to the eye degree, and use "accurate hair follicle transplantation," transplant, extract this region thin and soft hair follicle, then the pillow portion draws healthy sturdy hair follicle and draws back primary hairline position surplus hair follicle sturdy degree unanimous basically, just so can effectively avoid appearing linking unnatural area, let the hair planting effect more natural pleasing to the eye.
The fine and soft hair follicles are extracted from the primary hairline positions, so that the engagement degree can be effectively improved, the density and the naturalness can be enhanced, the nutrition competition between the fine and soft hair follicles and the transplanted hair follicles after the traditional hair transplantation operation can be effectively avoided, if the traditional operation mode is adopted, the fine and soft hair follicles belong to the primary hair follicles and have the original attributes, the nutrition obtaining efficiency after the transplantation greatly exceeds that of the transplanted hair follicles, the survival rate of the transplanted hair follicles is greatly influenced, namely the reason why a plurality of hairline planting patients are not ideal for recovery, the fine and soft hair follicles at the primary hairline positions are extracted by the gold hair follicle transplantation operation, the rest hairs are healthy hair follicles, the nutrition obtaining values of the hair follicles are relatively constant, the problem of a large amount of nutrition contention can not be generated, the effect on the recovery of newly transplanted hairs is good, and the fine and soft hair follicles are transplanted to the front end or the central position of the whole transplantation area, therefore, the transplanted hair follicles are similar in the competing ability, so that the condition of unbalanced nutrition distribution does not exist, and the survival rate of the hair follicles is effectively increased.
The transplantation patient needing encryption transplants the fine and soft hair follicles after processing and culturing into the area needing encryption, and the aperture of the fine and soft hair is small, so that the fine and soft hair follicles can be replanted in the gaps after the thick and strong hair follicles are planted, and better density can be achieved. The hair of the transplanted patient grows more naturally, and the rational effect is achieved.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. A method for transplanting a golden hair follicle, which is characterized by comprising the following steps:
extracting fine and soft hair follicles from a hair line part of the forehead of a patient by using a hair follicle extractor;
extracting healthy hair follicles from the back occiput of the patient using a hair follicle extractor;
culturing the fine hair follicle and the healthy hair follicle extracted from the occiput in a culture medium at 0-3 deg.C;
transplanting the cultured healthy hair follicles of the occiput into an area to be planted, and connecting the healthy hair follicles with the primary hairs;
transplanting the cultured fine and soft hair follicle to the newly shaped hairline position to ensure the naturalness of the hairline.
2. The method for transplanting the hair follicle in the golden standard of claim 1, wherein the hair follicle extractor extracts the hair follicle with the caliber of 0.4mm-0.6mm, and the process of extracting the hair follicle is carried out in a sterile environment.
3. The method for transplanting the hair follicle as claimed in claim 2, wherein the hair follicle extractor perforates the hair follicle from the position of the hairline and the posterior occipital region, extracts the hair follicle with the hair, and separates the hair from the hair follicle to obtain the hair follicle stem cells in different parts.
4. The method for transplanting the golden hair follicle according to claim 1, wherein the fine and soft hair follicle belongs to a hair follicle in which a hair with a forehead hairline portion shorter than a normal hair follicle diameter is located, and the extracted fine and soft hair follicle stem cells are subjected to proliferation culture in a serum-free culture medium to obtain cultured serum-free hair follicle stem cells.
5. The method for transplanting the golden hair follicle as claimed in claim 1, wherein the healthy hair follicle extracted from the posterior occipital part is cultured by proliferation of stem cells in a serum-free culture medium to obtain serum-free cultured hair follicle stem cells, and the hair grown from the healthy hair follicle in the posterior occipital part is thicker than the hair grown from the delicate hair follicle.
6. The method for transplanting the hair follicle of claim 1, wherein the healthy hair follicle of the posterior occipital part is implanted to the area to be developed by multiple injections after culture and proliferation, and the injection implantation operation is performed at intervals by using the dispersed injection.
7. The method for transplanting the golden hair follicle as claimed in claim 1, wherein the fine soft hair follicle is implanted at a newly molded hairline position by injection, so that the fine soft hair follicle grows at the hairline position close to a native hairline.
8. The method for transplanting the golden hair follicle as claimed in claim 1, wherein the fine and soft hair follicles can be transplanted to an area needing to be encrypted, and the hair follicles after the thick and strong hair follicles are planted can be replanted to achieve higher planting density.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011310718.2A CN112274227A (en) | 2020-11-20 | 2020-11-20 | Golden hair follicle transplantation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011310718.2A CN112274227A (en) | 2020-11-20 | 2020-11-20 | Golden hair follicle transplantation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112274227A true CN112274227A (en) | 2021-01-29 |
Family
ID=74399383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011310718.2A Pending CN112274227A (en) | 2020-11-20 | 2020-11-20 | Golden hair follicle transplantation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112274227A (en) |
-
2020
- 2020-11-20 CN CN202011310718.2A patent/CN112274227A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20030161815A1 (en) | Cell delivery system | |
| US6399057B1 (en) | Method for the propagation of hair | |
| CN109908329A (en) | Hair follicle cell activity factor hair growth accelerating preparation production technique and application thereof | |
| CN108236614B (en) | Implant for biological transplantation for promoting hair growth | |
| CN106635967A (en) | Hair follicle stem cell transplantation method | |
| CN113564111B (en) | Method for culturing umbilical cord-derived mesenchymal stem cells in low-oxygen mode | |
| CN105112353A (en) | Mixed cultivation method of keratinocyte and melanocyte and application | |
| CN115627256B (en) | Multi-layer tissue engineering skin composed of hair follicle cells and preparation method and application thereof | |
| Inaba et al. | Androgenetic alopecia: modern concepts of pathogenesis and treatment | |
| CN107164310A (en) | Method for reconstructing hair follicle in vivo | |
| WO1999001034A1 (en) | Method for producing new hair growth | |
| CN101264344B (en) | Preparation of artificial skin containing hair follicle and artificial skin prepared by the same | |
| Lee et al. | Evaluation of survival rate after follicular unit transplantation using the KNU implanter | |
| CN109988742A (en) | Autologous fibroblasts cultural method | |
| JP7526405B2 (en) | Method for isolating and mass-producing hair papilla cells derived from scalp tissue | |
| WO2020096539A2 (en) | Hair transplantation technique that reduces the hair growth process and reaches a higher number of hair follicle | |
| CN108324993B (en) | Stem cell complex for inducing hair regeneration, preparation method and application thereof | |
| Caroli et al. | Current status of hair restoration surgery | |
| CN112274227A (en) | Golden hair follicle transplantation method | |
| CN112891517A (en) | Cytokine composition for promoting scalp hair follicle regeneration and preparation method and application thereof | |
| Lam et al. | The potential for fat transplantation | |
| Jiménez-Acosta et al. | Follicular unit hair transplantation: current technique | |
| JP2001302520A (en) | Method for regenerating hair by cultured hair papilla cell | |
| JPH11180878A (en) | Trichogenous inducer and trichogenic method | |
| KR102254911B1 (en) | Method for transplanting dermal papilla cells through perforation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210129 |