CN112273372B - Symmetrical jet cooling and rewarming workbench - Google Patents
Symmetrical jet cooling and rewarming workbench Download PDFInfo
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- CN112273372B CN112273372B CN202011170708.3A CN202011170708A CN112273372B CN 112273372 B CN112273372 B CN 112273372B CN 202011170708 A CN202011170708 A CN 202011170708A CN 112273372 B CN112273372 B CN 112273372B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a symmetrical jet cooling and rewarming workbench, and belongs to the technical field of biological low-temperature storage. The device comprises a shell, a jet mechanism, a clamping mechanism and a controller; the jet mechanism comprises a pair of jet generating mechanisms, namely an upper jet generating mechanism and a lower jet generating mechanism; the clamping mechanism comprises an electric cylinder, the electric cylinder is fixed outside the shell through an electric cylinder fixing plate, and a piston rod of the electric cylinder is fixedly connected with the upper jet flow generating mechanism to drive the upper jet flow generating mechanism to move relative to the lower jet flow generating mechanism; a sample box which is cooled or reheated is placed on a lower clamping plate of a lower jet flow generation mechanism, and an electric cylinder drives an upper clamping plate of the upper jet flow generation mechanism to move, so that the upper clamping plate and the lower clamping plate are correspondingly matched; starting an upper jet generator and a lower jet generator to symmetrically implement liquid nitrogen jet cooling or warm water jet rewarming operation on the sample box; the two sides of the sample box exchange heat simultaneously, so that the heat exchange efficiency is effectively improved, and the temperature gradients of the two sides of the sample box are reduced.
Description
Technical Field
The invention belongs to the technical field of biological low-temperature preservation, and particularly relates to a cooling and rewarming workbench.
Background
Vitrification preservation is an important method in biological low-temperature preservation, is applied to long-term preservation of germ cells, stem cells, rare plant seeds and the like, and has the technical difficulty that the preserved objects are cooled and rewarming at high speed. The jet impact heat exchange refers to that working media (such as liquid nitrogen, cooling water and the like) are impacted on the surface of a cooled object at a high speed to exchange heat, and because a jet boundary layer is extremely thin, the jet impact has high heat exchange efficiency, and is a key technology for realizing high-speed cooling or rewarming of a large-volume sample. The cooling or rewarming workbench is a device for cooling or rewarming a sample in vitrification preservation and comprises a jet generator and a clamping mechanism. The fluidic generator is the working medium export when the efflux is efflux, has important influence to efflux heat transfer effect, and current fluidic generator is perpendicular installation in sample top usually, and working medium only strikes at the sample surface from one side, and heat is slowly transferred to the opposite side from sample one side under this kind of unilateral efflux, has restricted heat exchange efficiency, and there is great temperature gradient in sample both sides, has reduced the survival rate of sample. Clamping machine constructs and is used for fixed sample to prevent that it from droing when the efflux from strikeing, and the manual clamping is used commonly among the prior art or presss from both sides tightly with equipment such as pneumatic cylinder, cylinder: positioning errors are easy to generate during manual clamping to influence the movement of the jet flow working medium, so that the consistency of the impact effect is poor, and the large potential safety hazard exists in manual operation of low-temperature equipment due to the extremely low temperature of the jet flow cooling working medium; the hydraulic cylinder or the air cylinder has large vibration and low motion control precision, large pressure (clamping force) deviation is easy to generate, a sample falls off due to too small pressure, and the biological sample is damaged due to too large pressure.
Disclosure of Invention
The invention provides a symmetrical jet type cooling and rewarming workbench, aiming at solving the problems of low heat exchange efficiency, high temperature gradient, poor consistency of a clamping device, low safety, large vibration, low pressure precision and the like caused by only single-side jet flow in the conventional cooling and rewarming workbench.
A symmetrical jet type cooling and rewarming workbench comprises a machine shell 1, a jet mechanism, a clamping mechanism and a controller;
the jet flow mechanism comprises a pair of jet flow generating mechanisms, namely an upper jet flow generating mechanism and a lower jet flow generating mechanism; the upper jet flow generating mechanism comprises an upper jet flow generator 13 and an upper splint 3, and the lower jet flow generating mechanism comprises a lower jet flow generator 14 and a lower splint 2; the lower jet flow generation mechanism is fixed in the machine shell 1, the upper jet flow generation mechanism is movably positioned in the machine shell 1, and a jet orifice of the upper jet flow generator 13 is vertically corresponding to a jet orifice of the lower jet flow generator 14;
the clamping mechanism comprises an electric cylinder 9, the electric cylinder 9 is fixedly arranged outside the casing 1, a piston rod 6 of the electric cylinder 9 extends into the casing 1 and is fixedly connected with the upper jet flow generating mechanism to drive the upper jet flow generating mechanism to move relative to the lower jet flow generating mechanism;
placing the cooled or rewarmed sample box on the lower clamping plate 2 of the lower jet flow generation mechanism, starting the controller, and driving the upper clamping plate 3 of the upper jet flow generation mechanism to move through the electric cylinder 9 to realize the corresponding matching clamping of the upper clamping plate 3 and the lower clamping plate 2; and starting the upper jet flow generator 13 and the lower jet flow generator 14 to symmetrically implement liquid nitrogen jet flow cooling or warm water jet flow rewarming operation on the sample box.
The technical scheme for further limiting is as follows:
the upper jet flow generation mechanism also comprises a transition plate 5, the transition plate 5 is parallel to the upper clamping plate 3, and the transition plate 5 and the upper clamping plate 3 are fixedly connected by more than two connecting rods; the upper jet flow generator 13 is fixedly arranged on the upper splint 3 and is positioned between the transition plate 5 and the upper splint 3; a through hole is formed in the middle of the upper splint 3, and a jet port of the upper jet generator 13 is correspondingly communicated with the through hole of the upper splint 3; a piston rod 6 of the electric cylinder 9 extends into the machine shell 1 and is fixedly connected with the transition plate 5 through a threaded connecting piece.
The lower jet generator 14 is fixedly arranged on the lower splint 2; a through hole is formed in the middle of the lower splint 2, and a jet port of the lower jet generator 14 is correspondingly communicated with the through hole of the lower splint 2; the lower splint 2 is fixed in the casing 1 by more than two fixing rods.
The clamping mechanism further comprises an electric cylinder fixing plate 8, a lower fixing plate 7 and an upper fixing plate 12; the electric cylinder 9 is fixedly arranged outside the casing 1 through an electric cylinder fixing plate 8; the lower fixing plate 7 which is parallel to the electric cylinder fixing plate 8 is fixedly arranged in the machine shell 1; the upper fixing plate 12 is positioned outside the axial end part of the electric cylinder 9 and is fixedly connected with the machine shell 1 and the lower fixing plate 7 through four bolts; a pressure sensor 11 and a buffer spring 10 are sequentially arranged between the upper fixing plate 12 and the axial end part of the electric cylinder 9.
The distance between the upper clamping plate 3 and the lower clamping plate 2 is 10 mm.
The electric cylinder 9 is a servo electric cylinder, the maximum stroke of the piston rod is 50mm, and the speed is 0-60 mm/s.
During operation, the jet time of the upper jet generator 13 and the jet time of the lower jet generator 14 are both 2-10s, and the jet pressure between the upper splint 3 and the lower splint 2 is 10-100N.
The buffer spring 10 is a compression spring.
The beneficial technical effects of the invention are embodied in the following aspects:
1. the invention adopts the mode of installing jet flow generators on both sides of the sample to generate symmetrical jet flow, so that both sides of the sample exchange heat simultaneously, and under the condition of unchanging system thermal resistance, two heat sources are added simultaneously, compared with the heat exchange energy on one side, the heat exchange efficiency is effectively improved by at least 50 percent, simultaneously the temperature on both sides of the sample can be synchronously changed, and the temperature gradient on both sides is reduced to below 1 percent; the electric cylinder is used as a power element, the pressure control precision is improved to 1% from 10% of that of a traditional cylinder workbench by adjusting the motion mode of the electric cylinder through data of the pressure sensor, the vibration of equipment can be effectively reduced, the safety and consistency of the equipment are improved, and the problems of poor consistency, low safety, large vibration, low pressure precision and the like of the traditional method are effectively solved.
2. Adopt two-way jet generator to produce the symmetry efflux, through the pressure that pressure sensor perception sample box receives to the speed of travel and the distance of automatically regulated electronic jar make it press from both sides the sample box tightly according to predetermined pressure value, avoid pressure too big to cause the damage to the sample, sample box when also can preventing the efflux impact drops. In addition, electronic jar can promote splint fast and make the sample box break away from the workstation in several seconds, makes things convenient for the quick installation and the uninstallation of sample box, and this can effectively avoid the adverse effect that external environment brought the low temperature sample heating.
Drawings
FIG. 1 is a schematic view of the structure of the present invention.
Fig. 2 is a schematic view of the working state of the present invention.
Sequence numbers in the upper figure: 1. the device comprises a machine shell 1, a lower clamping plate 2, an upper clamping plate 3, a locking nut 4, a transition plate 5, a piston rod 6, a lower fixing plate 7, an electric cylinder fixing plate 8, an electric cylinder 9, a buffer spring 10, a pressure sensor 11, an upper fixing plate 12, an upper jet generator 13, a lower jet generator 14 and a sample box 15.
Detailed Description
The invention will now be further described by way of example with reference to the accompanying drawings.
Example 1
Referring to fig. 1, a symmetrical jet cooling and rewarming workbench comprises a machine shell 1, a jet mechanism, a clamping mechanism and a controller.
The jet mechanism comprises a pair of jet generating mechanisms, namely an upper jet generating mechanism and a lower jet generating mechanism.
The upper jet flow generating mechanism is movably positioned in the machine shell 1; the upper jet flow generating mechanism comprises an upper jet flow generator 13, an upper splint 3 and a transition plate 5; the transition plate 5 is parallel to the upper splint 3, and the transition plate 5 and the upper splint 3 are fixedly connected by four connecting rods. The upper jet flow generator 13 is fixedly arranged on the upper splint 3 and is positioned between the transition plate 5 and the upper splint 3; a through hole is formed in the middle of the upper splint 3, and a jet port of the upper jet generator 13 is correspondingly communicated with the through hole of the upper splint 3; a piston rod 6 of the electric cylinder 9 extends into the machine shell 1 and is fixedly connected with a transition plate 5 through a threaded connecting piece, and the upper jet flow generating mechanism is driven to move relative to the lower jet flow generating mechanism.
The lower jet flow generating mechanism is fixed in the machine shell 1 and comprises a lower jet flow generator 14 and a lower splint 2; the lower jet generator 14 is fixedly arranged on the lower splint 2; a through hole is formed in the middle of the lower splint 2, and a jet port of the lower jet generator 14 is correspondingly communicated with the through hole of the lower splint 2; the lower clamping plate 2 is fixed in the machine shell 1 through four fixing rods.
The jet orifice of the upper jet generator 13 and the jet orifice of the lower jet generator 14 correspond up and down, and the distance between the upper splint 3 and the lower splint 2 is 10 mm.
The clamping mechanism comprises an electric cylinder 9, an electric cylinder fixing plate 8, a lower fixing plate 7 and an upper fixing plate 12. The electric cylinder 9 is a servo electric cylinder, the maximum stroke of the piston rod is 50mm, and the speed is 0-60 mm/s. The electric cylinder 9 is fixedly arranged outside the casing 1 through an electric cylinder fixing plate 8; the lower fixing plate 7 which is parallel to the electric cylinder fixing plate 8 is fixedly arranged in the machine shell 1; the upper fixing plate 12 is positioned outside the axial end part of the electric cylinder 9 and is fixedly connected with the machine shell 1 and the lower fixing plate 7 through four bolts; a pressure sensor 11 and a buffer spring 10 are sequentially arranged between the upper fixing plate 12 and the axial end part of the cylinder body of the electric cylinder 9; the impact spring 10 is a compression spring.
Placing a cooled or rewarmed sample box 15 on the lower clamping plate 2 of the lower jet flow generation mechanism, starting the controller, and driving the upper clamping plate 3 of the upper jet flow generation mechanism to move through the electric cylinder 9 to realize the corresponding matching clamping of the upper clamping plate 3 and the lower clamping plate 2; and simultaneously starting the upper jet flow generator 13 and the lower jet flow generator 14 to symmetrically implement liquid nitrogen jet flow cooling or warm water jet flow rewarming operation on the sample box 15. The jet time of the upper jet generator 13 and the lower jet generator 14 is 2-10s, and the jet pressure between the upper splint 3 and the lower splint 2 is 10-100N.
Example 2: loading of biological samples
Referring to fig. 2, the specific loading operation steps are as follows:
1. a biological sample (e.g., stem cells, etc.) to be preserved is loaded into the sample cartridge, and the sample cartridge is placed on the lower plate.
2. And starting a power supply, setting a pressure value (within a set range of 10-100N) and ventilation time (within a set range of 2-10 s) on a controller, and confirming the start of clamping.
3. The stepping motor of the electric cylinder (the maximum stroke is 50mm, the precision is +/-0.05 mm, and the speed is 0' 60 mm/s) starts to work, the screw rod in the electric cylinder drives the slide rod of the electric cylinder to move downwards, and at the moment, the transition plate and the upper clamping plate synchronously move downwards.
4. The movement of the slide bar makes the cylinder body of the electric cylinder move upwards, continuously compresses the buffer spring (ripple spring, used for transmitting pressure and reducing vibration) and makes the pressure sensor (measuring range 0-10Kg, precision 0.3%) bear force.
5. The pressure sensor detects a pressure value and transmits data to the controller for operation, and the controller calculates the stroke and the speed of the next electric cylinder.
6. When the data of the pressure sensor is equal to the preset pressure value, the electric cylinder is controlled to stop moving and self-lock, the sample box is clamped on the clamping plate, and clamping is finished.
Example 3: symmetric jet cooling of samples
The specific symmetrical jet cooling operation steps are as follows:
1. the samples were loaded as in example 1.
2. And opening an external gas valve to enable high-pressure gas to push liquid nitrogen to be ejected from jet flow generators on two sides at a high speed to impact on the sample box, and enabling the liquid nitrogen to be heated and rapidly changed into heat to absorb the heat, so that the temperature of the sample is rapidly reduced to-196 ℃.
3. The automatic upward motion of electronic jar slide bar (quick motion, time about 1~2 seconds) when the time of ventilating finishes, conveniently takes out the sample box, avoids the sample temperature too fast to rise again.
4. And quickly transferring the cooled sample box to a liquid nitrogen storage tank for preservation.
Example 4: symmetrical jet rewarming of cooled samples
The specific symmetrical jet rewarming operation steps are as follows:
1. the sample box was removed from the liquid nitrogen storage tank and the sample loaded as shown in example 1.
2. And an external air valve is opened, so that the high-pressure air pushes the distilled water with the temperature of about 40 ℃ to be ejected at high speed from the jet flow generators on the two sides and impact the distilled water on the sample box, and as the jet flow impact boundary layer is extremely thin, the convection heat exchange efficiency is high, the temperature of the sample can be quickly increased from-196 ℃ to about 20 ℃, and the cell recrystallization can be avoided so as to improve the survival rate of the cells.
3. And when the ventilation time is over, the electric cylinder slide rod automatically moves upwards, so that the sample box is conveniently taken out.
4. And taking down the rewarmed sample box and collecting the sample in the box, and treating the sample box for research or clinical use.
Claims (4)
1. The utility model provides a symmetry efflux formula cooling rewarming workstation, includes casing (1), efflux mechanism, clamping machine structure and controller, its characterized in that:
the jet flow mechanism comprises a pair of jet flow generating mechanisms, namely an upper jet flow generating mechanism and a lower jet flow generating mechanism; the upper jet flow generating mechanism comprises an upper jet flow generator (13) and an upper splint (3), and the lower jet flow generating mechanism comprises a lower jet flow generator (14) and a lower splint (2); the lower jet flow generation mechanism is fixed in the machine shell (1), the upper jet flow generation mechanism is movably positioned in the machine shell (1), and a jet flow port of the upper jet flow generator (13) is vertically corresponding to a jet flow port of the lower jet flow generator (14);
the upper jet flow generation mechanism also comprises a transition plate (5), the transition plate (5) is parallel to the upper clamping plate (3), and the transition plate (5) and the upper clamping plate (3) are fixedly connected by more than two connecting rods; the upper jet flow generator (13) is fixedly arranged on the upper splint (3) and is positioned between the transition plate (5) and the upper splint (3); a through hole is formed in the middle of the upper splint (3), and a jet port of the upper jet generator (13) is correspondingly communicated with the through hole of the upper splint (3); a piston rod (6) of the electric cylinder (9) extends into the shell (1) and is fixedly connected with the transition plate (5) through a threaded connecting piece;
the lower jet generator (14) is fixedly arranged on the lower splint (2); a through hole is formed in the middle of the lower splint (2), and a jet port of the lower jet generator (14) is correspondingly communicated with the through hole of the lower splint (2); the lower splint (2) is fixed in the casing (1) through more than two fixing rods;
the clamping mechanism comprises an electric cylinder (9), the electric cylinder (9) is fixedly arranged outside the casing (1) through an electric cylinder fixing plate (8), a piston rod (6) of the electric cylinder (9) extends into the casing (1) and is fixedly connected with the upper jet flow generating mechanism to drive the upper jet flow generating mechanism to move relative to the lower jet flow generating mechanism;
the clamping mechanism further comprises an electric cylinder fixing plate (8), a lower fixing plate (7) and an upper fixing plate (12); the electric cylinder (9) is fixedly arranged outside the casing (1) through an electric cylinder fixing plate (8); the lower fixing plate (7) which is correspondingly parallel to the electric cylinder fixing plate (8) is fixedly arranged in the shell (1); the upper fixing plate (12) is positioned outside the axial end part of the electric cylinder (9) and is fixedly connected with the machine shell (1) and the lower fixing plate (7) through four bolts; a pressure sensor (11) and a buffer spring (10) are sequentially arranged between the upper fixing plate (12) and the axial end part of the cylinder body of the electric cylinder (9);
placing the sample box which is cooled or reheated on a lower clamping plate (2) of a lower jet flow generation mechanism, starting a controller, and driving an upper clamping plate (3) of an upper jet flow generation mechanism to move through an electric cylinder (9) to realize corresponding matching clamping of the upper clamping plate (3) and the lower clamping plate (2); starting an upper jet generator (13) and a lower jet generator (14) to symmetrically implement liquid nitrogen jet cooling or warm water jet rewarming operation on the sample box;
when the jet flow generating device works, the jet flow time of the upper jet flow generator (13) and the lower jet flow generator (14) is 2-10s, and the jet flow pressure between the upper splint (3) and the lower splint (2) is 10-100N.
2. The symmetrical jet cooling and rewarming workbench according to claim 1, characterized in that: the distance between the upper clamping plate (3) and the lower clamping plate (2) is 10 mm.
3. The symmetrical jet cooling and rewarming workbench according to claim 1, characterized in that: the electric cylinder (9) is a servo electric cylinder, the maximum stroke of the piston rod is 50mm, and the speed is 0-60 mm/s.
4. The symmetrical jet cooling and rewarming workbench according to claim 1, characterized in that: the buffer spring (10) is a compression spring.
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| CN202010820571 | 2020-08-14 | ||
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| CN112273372A CN112273372A (en) | 2021-01-29 |
| CN112273372B true CN112273372B (en) | 2021-10-12 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614523A (en) * | 2016-11-17 | 2017-05-10 | 电子科技大学 | Vitrification preservation-oriented cooling/rewarming working medium driving device and control method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614523A (en) * | 2016-11-17 | 2017-05-10 | 电子科技大学 | Vitrification preservation-oriented cooling/rewarming working medium driving device and control method thereof |
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