CN112268880A - Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control - Google Patents
Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control Download PDFInfo
- Publication number
- CN112268880A CN112268880A CN202011020063.5A CN202011020063A CN112268880A CN 112268880 A CN112268880 A CN 112268880A CN 202011020063 A CN202011020063 A CN 202011020063A CN 112268880 A CN112268880 A CN 112268880A
- Authority
- CN
- China
- Prior art keywords
- light source
- placing
- fluorescence detection
- wavelength led
- wavelength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
Abstract
The invention relates to PC control-based multi-wavelength LED transmission light source fluorescence detection equipment which comprises a placing box, wherein a multi-wavelength LED light source serving as a transmission light source is placed in the placing box, the multi-wavelength LED light source comprises an LED light plate, LED lamp beads arranged on the LED light plate and light transmission plates with corresponding wavelengths arranged on the LED lamp beads, the LED light plate also comprises a first interface and a second interface which are arranged on the same side of the placing strip, the first interface and the second interface are respectively a power supply interface and a PC control interface, and the PC control interface is electrically connected with a computer. The sample to be subjected to fluorescence detection is placed in the placing box, the multi-wavelength LED light source and the camera module are controlled by the computer, the fluorescence detection process is shot and controlled by the camera module, the multi-wavelength LED light source serves as a transmission light source, the wavelength of the light source can be changed without replacing a lamp tube, and the fluorescence detection device is more convenient to use. And the computer is convenient for the control of various excitation light sources, and realizes the automatic control of fluorescence detection.
Description
Technical Field
The invention belongs to the technical field of biological science fluorescence detection, and particularly relates to PC (personal computer) -control-based multi-wavelength LED (light-emitting diode) transmission light source fluorescence detection equipment and method.
Background
In the field of life sciences, there is a method for detecting nucleic acids, proteins, compounds, and the like, which is mostly dyed or contained by itself using ultraviolet light-excited excitation in the ultraviolet band of the transmission system, and excites a fluorescent material having an excitation wavelength in the ultraviolet band. In the other part, a fluorescent plate having a specific wavelength is excited by ultraviolet rays, the fluorescent plate generates transmitted excitation light having a specific wavelength when excited, and a fluorescent material contained in a sample emits light having a specific wavelength when excited, whereby a target sample such as a nucleic acid, a protein, or a compound is detected. At present, a transmission ultraviolet light source on the market is used as an excitation light source in detection of nucleic acid, protein, compounds and the like, and all traditional filament type glass tube ultraviolet lamps are used, and because the traditional filament type glass tube ultraviolet lamps have short normal service life (continuously lighted for 2000 hours), are fragile and standardize the length and the diameter of the lamps, instruments provided with the traditional ultraviolet lamps need to be frequently replaced after sales, the instruments are likely to need to be installed on site by users, and the miniaturization and the heterotyping of the size design of the instruments are difficult to select. It is disadvantageous for the development of instrumentation. And when different fluorescence tests need to be carried out, different fluorescent plates need to be disassembled and assembled to realize the replacement of different wavelengths. And the fluorescence detection process is difficult to realize automatic control.
Disclosure of Invention
1. Technical problem to be solved by the invention
The invention aims to solve the problems that the existing fluorescence detection equipment is inconvenient to use, poor in effect and difficult to realize automatic control in the fluorescence detection process because excitation light sources with different wavelengths need to be changed by replacing a lamp tube in the use process.
2. Technical scheme
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention relates to PC control-based multi-wavelength LED transmission light source fluorescence detection equipment which comprises a placing box, wherein a multi-wavelength LED light source serving as a transmission light source is placed in the placing box, the multi-wavelength LED light source comprises an LED light plate, an LED lamp bead arranged on the LED light plate and a light transmission plate arranged on the LED lamp bead, the LED light plate also comprises a first interface and a second interface which are arranged on the same side of the placing strip, the first interface and the second interface are respectively a power supply interface and a PC control interface, and the PC control interface is electrically connected with a computer.
Preferably, the wavelength range of the multi-wavelength LED light source is 200-820 nm and white light.
Preferably, the multi-wavelength LED light source is an LED lamp bead with adjustable wavelength.
Preferably, the multi-wavelength LED light source is a plurality of fixed-wavelength LED light beads with different wavelengths or an LED light panel provided with a plurality of fixed-wavelength LED light beads with different wavelengths.
Preferably, the multi-wavelength LED light source is a plurality of LED lamp beads with adjustable wavelengths or an LED light board provided with a plurality of LED lamp beads with adjustable wavelengths.
Preferably, the multi-wavelength LED light source is at least two of blue light, ultraviolet light and other wavelengths except for white light.
Preferably, still including shooting the storehouse, it is provided with and places the chamber to place the bottom of the case portion, it sets up in placing the case top and just to placing the chamber setting to shoot the storehouse, shoot the storehouse with place the case and link up the setting.
Preferably, the placing box is provided with a sealing door, one side of the sealing door, which is close to the placing box, is provided with a light screen, and when the sealing door is closed, the light screen covers an opening of the placing box.
Preferably, the bottom of the placing box is provided with a placing cavity, the multi-wavelength LED light source is arranged in the placing cavity, and the placing cavity is covered with light-transmitting plates with corresponding wavelengths.
Preferably, the wavelength of the multi-wavelength LED light source is at least two of 250-260nm, 290-320 nm, 365nm, 460-480nm, 530-540nm, 620nm, 660-720nm and 750-820 nm.
Preferably, shoot the storehouse and be equipped with and place the mouth and shoot the chamber, the horizontal cross-sectional area who shoots the chamber increases in proper order along the direction of being close to and placing the case, shoot the chamber with shoot the storehouse through connection.
Preferably, the side walls of the shooting cavity, which are close to the placing cavity, are provided with light absorption coatings.
Preferably, the side wall of the placing box is provided with an inwards concave sliding groove.
Preferably, a placing groove is formed in the placing cavity, a placing strip is arranged at the bottom of the LED light plate, and the shape of the placing strip is matched with that of the placing groove, so that the placing strip is embedded into the placing groove.
Preferably, the placing cavity is further provided with a first interface placing groove and a second interface placing groove, and when the LED light panel is placed in the placing cavity, the first interface and the second interface are respectively embedded into the first interface placing groove and the second interface placing groove.
Preferably, the horizontal cross section of the shooting cavity is circular or rectangular.
Preferably, a vertical axis of the shooting cavity is collinear with a vertical axis of the placing cavity.
Preferably, still include the shooting device, the shooting device is including the control and interface module, fixed module and the camera module that connect gradually, the camera module sets up in placing the mouth, the camera of camera module passes and places the mouth and go deep into and shoot the intracavity, control and interface module are connected with computer and camera module electricity simultaneously.
Preferably, the horizontal cross-sectional area of the shooting cavity at the maximum horizontal cross-sectional area is larger than the horizontal cross-sectional area of the placing cavity.
Preferably, the side wall and the inner wall of the sliding chute are provided with light absorption coatings.
Preferably, the number of the placing grooves is two, the placing grooves are communicated with each other, and the joint of the two placing grooves is the center of the placing groove.
Preferably, the number of the placing grooves is at least three, at least two crossed placing grooves exist in the at least three placing grooves, and the connecting position is the center of the placing grooves.
Preferably, the LED light board is provided with a notch, and the notch is semicircular or crescent.
Preferably, the edge of the fixing module covers the placing opening, a fixing groove is formed in the edge of the placing opening, a fixing protrusion is arranged on the edge of one side of the fixing module, which is in contact with the placing opening, and the fixing protrusion is embedded into the fixing groove due to the shape matching of the fixing protrusion and the fixing groove.
Preferably, the fixed slot encircles place the mouth setting and end to end.
Preferably, the areas, which cover the placing openings, of the edges of the fixing modules are provided with light absorption coatings.
A multi-wavelength LED transmission light source fluorescence detection method based on PC control adopts the detection equipment, a sample to be subjected to fluorescence detection is placed in a placing box, a computer is used for controlling a multi-wavelength LED light source and a camera module, and the camera module is used for shooting and controlling the fluorescence detection process.
Preferably, when a sample to be subjected to fluorescence detection is placed in the placing box, the camera module is started after the placing box is closed, the camera module shoots that the placing box is completely sealed if no light source exists in the placing box, the computer controls the multi-wavelength LED light source to emit exciting light, the exciting light excites corresponding fluorescent materials to generate corresponding emitting light after penetrating through the sample, the emitting light reaches the camera module and is shot by the camera module, and when the camera module shoots that the fluorescence condition in the sample reaches a set value, the computer controls the multi-wavelength LED light source to be closed to finish the fluorescence detection process.
Preferably, the computer adjusts the corresponding excitation wavelength emitted by the multi-wavelength LED light source according to different excitation light sources required by the sample to be subjected to fluorescence detection.
3. Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
the invention relates to PC control-based multi-wavelength LED transmission light source fluorescence detection equipment which comprises a placing box, wherein a multi-wavelength LED light source serving as a transmission light source is placed in the placing box, the multi-wavelength LED light source comprises an LED light plate, an LED lamp bead arranged on the LED light plate and a light transmission plate arranged on the LED lamp bead, the LED light plate also comprises a first interface and a second interface which are arranged on the same side of the placing strip, the first interface and the second interface are respectively a power supply interface and a PC control interface, and the PC control interface is electrically connected with a computer. The sample of treating fluorescence detection is placed and is being placed the incasement, through computer control multi-wavelength LED light source and camera module, camera module shoots fluorescence detection process and controls, through regarding multi-wavelength LED lamp as transmission light source, not only can promote the life of light source, reduce the loss cost, and the suitability of LED lamp is good, the circular telegram response time is short, and multi-wavelength LED light source is as transmission light source, need not just can realize the transform of light source wavelength through changing the fluorescent tube, and is more convenient, for traditional light pipe type transmission light source, because the light that multi-wavelength LED light source sent is more even and intensity is high, form dark background easily, obtain good contrast, be favorable to the observation and the control to fluorescence reaction. And the computer is convenient for the control of various excitation light sources, and realizes the automatic control of fluorescence detection.
Drawings
FIG. 1 is a schematic structural diagram of a PC-control-based multi-wavelength LED transmission light source fluorescence detection apparatus according to the present invention;
FIG. 2 is a schematic view of the apparatus of the present invention with the camera removed;
FIG. 3 is a front view of the present invention;
FIG. 4 is a schematic view of the structure of the present invention with the light-transmitting plate and the light source removed;
FIG. 5 is a schematic view of the structure of the light-transmitting plate of the present invention;
FIG. 6 is a top view of the capture chamber of the present invention;
fig. 7 is a schematic structural diagram of an LED light panel according to the present invention.
The reference numerals in the schematic drawings illustrate:
100. a shooting bin; 110. a placement port; 111. a placement groove; 120. a shooting cavity;
200. placing a box; 210. a baffle plate; 220. a light-transmitting plate; 230. a side wall; 231. a chute; 240. a sealing door; 241. a visor; 250. a placement chamber; 251. a placement groove; 252. an interface placing groove I; 253. an interface placing groove II; 260. a light source; 261. an LED light panel; 262. LED lamp beads; 263. placing the strips; 264. a notch; 265. an interface I; 266. interface two;
300. a photographing device; 310. a control and interface module; 320. a fixed module; 330. and a camera module.
Detailed Description
In order to facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which may be embodied in many different forms and are not limited to the embodiments described herein, but rather are provided for the purpose of providing a more thorough disclosure of the invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; the terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention; as used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
Referring to fig. 1 to 7, the multi-wavelength LED transmission light source fluorescence detection apparatus based on PC control according to the embodiment includes a placing box 200, a multi-wavelength LED light source 260 serving as a transmission light source is placed in the placing box 200, the multi-wavelength LED light source 260 includes an LED light plate 261, an LED light bead 262 disposed on the LED light plate 261, and a light transmission plate 220 disposed on the LED light bead, the LED light plate 261 further includes a first interface 265 and a second interface 266 disposed on the same side of the placing strip 263, the first interface 265 and the second interface 266 are a power interface and a PC control interface, respectively, and the PC control interface is electrically connected to a computer. Through PC control multi-wavelength LED light source 260, can realize quick adjustment and intelligent regulation to multi-wavelength LED light source 260, and through regarding multi-wavelength LED lamp as transmission light source, not only can promote the life of light source, reduce the loss cost, and the suitability of LED lamp is good, the circular telegram response time is short, and multi-wavelength LED light source 260 is as transmission light source, need not just can realize the transform of light source wavelength through changing the fluorescent tube, and is more convenient, for traditional lamp cast transmission light source, because the light that multi-wavelength LED light source 260 sent is more even and intensity is high, form dark background easily, obtain good contrast, be favorable to observation and control to fluorescence reaction. And because the LED lamp bead 262 has the characteristics of low energy consumption and small volume, the miniaturization and the heterotyping of instrument design and the possibility of respectively using a plurality of uniform excitation wavelength light sources in the same sample plate size become possible. The long life and non-fragile nature of LED light source 260 make the integration of non-later stage installation of instrument design and reduce the aspect such as after-market frequency of service have very big advantage.
The multi-wavelength LED light source 260 of the present embodiment has a wavelength range of 200-820 nm and white light. The multi-wavelength LED light source 260 is an LED lamp bead 262 with adjustable wavelength. The wavelength required by the fluorescence test can be conveniently and quickly obtained under the condition of not replacing the lamp source. The wavelength of the multi-wavelength LED light source 260 is at least two of 250-260nm, 290-320 nm, 365nm, 460-480nm, 530-540nm, 620nm, 660-720nm and 750-820 nm.
The multi-wavelength LED light source 260 of the present embodiment is at least two types of wavelengths, including white light, blue light, and ultraviolet light.
The multi-wavelength LED light source 260 of this embodiment includes LED worn-out fur 261 and sets up LED lamp pearl 262 on LED worn-out fur 261, through with LED lamp pearl 262 integrated on LED worn-out fur 261, is convenient for adjust luminance and wavelength that LED lamp pearl 262 lighted, thereby conveniently controls the intensity of transmitted light and is convenient for observe the fluorescence reaction.
The equipment of this embodiment still includes shoots storehouse 100, shoot storehouse 100 set up in placing case 200 top and just to placing the chamber 250 setting, shoot storehouse 100 with place case 200 and link up the setting.
The placing box 200 of this embodiment is equipped with sealing door 240, one side that sealing door 240 is close to placing box 200 is equipped with light screen 241, when sealing door 240 closed, light screen 241 covers the opening of placing box 200, thereby can effectually prevent that sealing door 240 when closed, sealing door 240 and the clearance department of placing box 200 have light entering place the box 200 in thereby influence the fluorescence reaction monitoring process.
The bottom of the placing box 200 is provided with a placing cavity 250, the multi-wavelength LED light source 260 is arranged in the placing cavity 250, the placing cavity 250 is covered with a light-transmitting plate 220, the light-transmitting plate 220 can effectively isolate the placing cavity 250, a reaction sample in the placing box 200 is prevented from permeating liquid into the placing cavity 250 and contacting with the multi-wavelength LED light source 260 to cause damage, light rays emitted by the multi-wavelength LED light source 260 can be prevented from being blocked by the light-transmitting plate 220, and the light-transmitting plate 220 can also be a light-transmitting device with a light-gathering function.
Shoot chamber 120 and be close to all be equipped with the extinction coating on placing the lateral wall of chamber 250, can prevent that the lateral wall of shooting chamber 120 from reflecting the exciting light that multi-wavelength LED light source 260 sent because the exciting light of reflection can interfere fluorescence to the picture that leads to the camera to shoot is inaccurate and unclear, and then influences the testing process and the result judgement to fluorescence reaction.
The side wall 230 of the placing box 200 is provided with an inward concave sliding groove 231, the partition board is placed through the sliding groove 231 and the sample to be detected is placed, and the sliding groove 231 of the inward concave groove can prevent exciting light from being shielded so as to influence the fluorescence detection process.
The placing groove 251 is arranged in the placing cavity 250, the placing strip 263 is arranged at the bottom of the LED light plate 261, and the shape of the placing strip 263 is matched with that of the placing groove 251, so that the placing strip 263 is embedded into the placing groove 251, and the LED light plate 261 is convenient to place and fix.
The placing cavity 250 is further internally provided with a first interface placing groove 252 and a second interface placing groove 253, the LED optical plate 261 further comprises a first interface 265 and a second interface 266 which are arranged on the same side of the placing strip 263, and when the LED optical plate 261 is placed in the placing cavity 250, the first interface 265 and the second interface 266 are respectively embedded into the first interface placing groove 252 and the second interface placing groove 253.
The horizontal cross section of the shooting cavity 120 is circular or rectangular, which is beneficial to ensuring the shooting angle of the camera, so that the shooting by the camera is more accurate and comprehensive. The vertical axis of the shooting cavity 120 is collinear with the vertical axis of the placing cavity 250, so that the camera can be over against the LED light board 261, and the shooting effect of the camera is further guaranteed.
The horizontal cross-sectional area of the maximum horizontal cross-sectional area of the shooting cavity 120 is larger than the horizontal cross-sectional area of the placing cavity 250, so that the shooting angle of the camera can contain the placing cavity 250 as much as possible, and the shooting effect of the camera is further ensured.
The side wall 230 and the inner wall of the sliding groove 231 are both provided with light absorption coatings, so that the excitation light emitted by the multi-wavelength LED light source 260 is prevented from being reflected, and the reflected excitation light interferes with the fluorescence, so that the picture shot by the camera is inaccurate and unclear, and the detection process and result judgment of the fluorescence reaction are influenced.
The number of the placing grooves 251 is two and they are communicated with each other, and the joint of the two placing grooves 251 is the center of the placing groove 251. Under being favorable to placing LED worn-out fur 261 and carrying out the prerequisite fixed to LED worn-out fur 261, further guarantee that LED worn-out fur 261 is located under the camera, guarantee the shooting effect of camera.
The edge of the fixing module 320 covers the placing opening 110, the edge of the placing opening 110 is provided with a fixing groove 111, the edge of one side of the fixing module 320 contacting with the placing opening 110 is provided with a fixing protrusion 321, and the shape of the fixing protrusion 321 is matched with the shape of the fixing groove 111 so that the fixing protrusion 321 is embedded into the fixing groove 111. When the shooting device 300 is placed on the shooting bin 100, the fixing protrusions 321 are embedded into the fixing grooves 111, so that the fixing modules 320 can be better fixed with the shooting bin 100, the fixing grooves 111 surround the placing openings 110 and are connected end to end, the fixing protrusions 321 are matched with the fixing grooves 111, and the fixing modules 320 and the shooting bin 100 can be well sealed. In addition, the edge of the fixing module 320 is provided with a light absorbing coating in the area covering the placing opening 110, which can further prevent the external relationship from entering the placing box 200 through the gap between the photographing device 300 and the photographing chamber 100
The LED light plate 261 of this embodiment is provided with a notch 264, and the notch 264 is in a semicircular shape or a crescent shape. The user can conveniently remove the LED light board 261 from the placing cavity 250 through the notch 264, and the first interface 265 and the second interface 266 can be used as a power interface or a control circuit interface.
Example 2
The basic structure of this embodiment is the same as that of embodiment 1, except that: the number of the placing grooves 251 is three, two crossing placing grooves 251 exist among the three placing grooves 251, and the connection point is the center of the placing groove 251.
Example 3
The basic structure of this embodiment is the same as that of embodiment 1, except that: the specific structure of multi-wavelength LED light source 260 is different, and the switching of different wavelengths can be realized to the LED lamp pearl 262 that only needs to correspond the wavelength when needs are adjusted not with the wavelength for the LED worn-out fur 261 of the fixed wavelength's of the fixed wavelength LED lamp pearl 262 that is equipped with a plurality of multi-wavelength LED light source 260 of this embodiment, and is more convenient and practical.
Example 4
The basic structure of this embodiment is the same as that of embodiment 1, except that: the specific structure of the multi-wavelength LED light source 260 is different, and the multi-wavelength LED light source 260 of this embodiment is an LED light plate 261 provided with a plurality of wavelength-adjustable LED lamp beads 262, and the adjustment of the whole light source is realized by adjusting the wavelength of the LED lamp beads 262, and the intensity of the light source is controlled by the number of the LED lamp beads 262.
Example 5
The method for detecting fluorescence of the multi-wavelength LED transmission light source based on PC control in the embodiment adopts the equipment in the embodiment 3 or 4, a sample to be subjected to fluorescence detection is placed in the placing box 200, the multi-wavelength LED light source 260 and the camera module 330 are controlled by the computer, and the camera module 330 shoots and controls the fluorescence detection process.
When a sample to be subjected to fluorescence detection is placed in the placing box 200, the placing box 200 is closed, the camera module 330 is started, the camera module 330 shoots the placing box 200 without a light source, then the placing box 200 is completely sealed, the computer controls the multi-wavelength LED light source 260 to emit excitation light, the excitation light excites corresponding fluorescent materials to generate corresponding emission light after penetrating through the sample, the emission light reaches the camera module 330 and is shot by the camera module 330, and when the camera module 330 shoots that the fluorescence condition in the sample reaches a set value, the computer controls the multi-wavelength LED light source 260 to be closed to complete the fluorescence detection process.
The computer adjusts the excitation light with the corresponding wavelength emitted by the multi-wavelength LED light source 260 according to the excitation light source required by the sample to be subjected to fluorescence detection.
The above-mentioned embodiments only express a certain implementation mode of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the present invention; it should be noted that, for those skilled in the art, without departing from the concept of the present invention, several variations and modifications can be made, which are within the protection scope of the present invention; therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (29)
1. The utility model provides a multi-wavelength LED transmission light source fluorescence check out test set based on PC control which characterized in that: including placing case (200), place multi-wavelength LED light source (260) as transmission light source in case (200), multi-wavelength LED light source (260) include LED worn-out fur (261) and set up LED lamp pearl (262) on LED worn-out fur (261) and set up light-passing board (220) on LED lamp pearl, LED worn-out fur (261) still including setting up in placing strip (263) interface one (265) and interface two (266) with one side, interface one (265) and interface two (266) are power source and PC control interface respectively, PC control interface electricity is connected with the computer.
2. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the wavelength range of the multi-wavelength LED light source (260) is 200-820 nm and white light.
3. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the multi-wavelength LED light source (260) is an LED lamp bead (262) with adjustable wavelength.
4. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the multi-wavelength LED light source (260) is an LED light plate (261) provided with a plurality of LED lamp beads (262) with different wavelengths and fixed wavelengths or provided with a plurality of LED lamp beads (262) with different wavelengths and fixed wavelengths.
5. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the multi-wavelength LED light source (260) is an LED lamp bead (262) with a plurality of adjustable wavelengths or an LED light plate (261) provided with the LED lamp bead (262) with the plurality of adjustable wavelengths.
6. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the multi-wavelength LED light source (260) is at least two of blue light, ultraviolet light and other wavelengths except for white light.
7. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: still including shooting storehouse (100), it is provided with and places chamber (250) to place case (200) bottom, it sets up in placing case (200) top and just to placing chamber (250) setting to shoot storehouse (100), shoot storehouse (100) with it link up the setting to place case (200).
8. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the storage box (200) is provided with a sealing door (240), one side, close to the storage box (200), of the sealing door (240) is provided with a light shielding plate (241), and when the sealing door (240) is closed, the light shielding plate (241) covers an opening of the storage box (200).
9. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 1, wherein: the bottom of the placing box (200) is provided with a placing cavity (250), the multi-wavelength LED light source (260) is arranged in the placing cavity (250), and the placing cavity (250) is covered with a light-transmitting plate (220).
10. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 2, wherein: the wavelength of the multi-wavelength LED light source (260) is 250-260nm, 290-320 nm, 365nm, 460-480nm, 530-540nm, 620nm, 660-720nm, 750-820nm and at least two species.
11. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 7, wherein: shoot storehouse (100) and be equipped with and place mouth (110) and shoot chamber (120), the horizontal cross-sectional area who shoots chamber (120) increases in proper order along the direction of being close to and placing case (200), shoot chamber (120) with shoot storehouse (100) through connection.
12. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 7, wherein: and light absorption coatings are arranged on the side walls of the shooting cavity (120) close to the placing cavity (250).
13. The PC-based multi-wavelength LED transmission light source fluorescence detection device as claimed in claim 8, wherein: the side wall (230) of the placing box (200) is provided with an inward concave sliding groove (231).
14. The PC-based multi-wavelength LED transmission light source fluorescence detection device of claim 9, wherein: a placing groove (251) is arranged in the placing cavity (250), a placing strip (263) is arranged at the bottom of the LED light board (261), and the shape of the placing strip (263) is matched with that of the placing groove (251) so that the placing strip (263) is embedded into the placing groove (251).
15. The PC-based multi-wavelength LED transmission light source fluorescence detection device of claim 9, wherein: the LED light board is characterized in that a first interface placing groove (252) and a second interface placing groove (253) are further formed in the placing cavity (250), and when the LED light board (261) is placed in the placing cavity (250), the first interface (265) and the second interface (266) are respectively embedded into the first interface placing groove (252) and the second interface placing groove (253).
16. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 11, wherein: the horizontal cross section of the shooting cavity (120) is circular or rectangular.
17. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 11, wherein: the vertical axis of the shooting cavity (120) is collinear with the vertical axis of the placing cavity (250).
18. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 11, wherein: the multifunctional photographing device is characterized by further comprising a photographing device (300), wherein the photographing device (300) comprises a control and interface module (310), a fixing module (320) and a photographing module (330) which are sequentially connected, the photographing module (330) is arranged in the placing opening (110), a camera of the photographing module (330) penetrates through the placing opening (110) and penetrates into the photographing cavity (120), and the control and interface module (310) is simultaneously electrically connected with the computer and the photographing module (330).
19. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 11, wherein: the horizontal cross-sectional area of the shooting cavity (120) at the maximum horizontal cross-sectional area is larger than that of the placing cavity (250).
20. The PC-based multi-wavelength LED transmission light source fluorescence detection apparatus of claim 13, wherein: and the side wall (230) and the inner wall of the sliding groove (231) are provided with light absorption coatings.
21. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 14, wherein: the number of the placing grooves (251) is two, the placing grooves are communicated with each other, and the connecting position of the two placing grooves (251) is the center of the placing grooves (251).
22. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 14, wherein: the number of the placing grooves (251) is at least three, at least two crossed placing grooves (251) exist in the at least three placing grooves (251), and the connecting position is the center of the placing grooves (251).
23. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 15, wherein: the LED light board (261) is provided with a notch (264), and the notch (264) is semicircular or crescent.
24. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus according to claim 18, wherein: the edge of fixed module (320) covers place mouthful (110), it is equipped with fixed slot (111) to place mouthful (110) edge, fixed module (320) and the one side edge of placing mouthful (110) contact are equipped with fixed arch (321), the shape of fixed arch (321) and the shape looks adaptation of fixed slot (111) make fixed arch (321) imbed fixed slot (111).
25. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus of claim 24, wherein: the fixing groove (111) is arranged around the placing opening (110) and is connected end to end.
26. The PC-controlled multi-wavelength LED transmission light source fluorescence detection apparatus of claim 24, wherein: the areas, covered by the edges of the fixed modules (320), of the placing openings (110) are provided with light absorption coatings.
27. A multi-wavelength LED transmission light source fluorescence detection method based on PC control is characterized in that: the detection device according to any one of the preceding claims 24 to 26, wherein the sample to be subjected to fluorescence detection is placed in the placement box (200), the multi-wavelength LED light source (260) and the camera module (330) are controlled by the computer, and the camera module (330) is used to image and control the fluorescence detection process.
28. The PC-control-based multi-wavelength LED transmission light source fluorescence detection method of claim 27, wherein: when a sample to be subjected to fluorescence detection is placed in the placing box (200), the placing box (200) is closed, the camera module (330) is started, the camera module (330) shoots that no light source exists in the placing box (200), then the placing box (200) is completely sealed, the computer controls the multi-wavelength LED light source (260) to emit exciting light, the exciting light penetrates through the sample and excites corresponding fluorescent materials to generate corresponding emitting light, the emitting light reaches the camera module (330) and is shot by the camera module (330), and when the camera module (330) shoots that the fluorescence condition in the sample reaches a set value, the computer controls the multi-wavelength LED light source (260) to be closed to finish the fluorescence detection process.
29. The PC-control-based multi-wavelength LED transmission light source fluorescence detection method of claim 27, wherein: the computer adjusts the multi-wavelength LED light source (260) to emit exciting light with corresponding wavelength according to different exciting light sources required by the sample to be subjected to fluorescence detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011020063.5A CN112268880A (en) | 2020-09-25 | 2020-09-25 | Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011020063.5A CN112268880A (en) | 2020-09-25 | 2020-09-25 | Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112268880A true CN112268880A (en) | 2021-01-26 |
Family
ID=74349606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011020063.5A Pending CN112268880A (en) | 2020-09-25 | 2020-09-25 | Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112268880A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001083090A (en) * | 1999-09-10 | 2001-03-30 | Fuji Photo Film Co Ltd | Excitation light source for micro titer plate |
| US20120298885A1 (en) * | 2011-05-26 | 2012-11-29 | Fujifilm Corporation | Fluorometric assay apparatus and fluorometric assay method |
| CN104655599A (en) * | 2013-11-19 | 2015-05-27 | 郑兆珉 | Fluorescent test paper, fluorescent excitation device and portable fluorescent analysis system |
| CN204556502U (en) * | 2015-04-17 | 2015-08-12 | 上海生物信息技术研究中心 | A kind of fluorescence imaging analysis system |
| CN105158204A (en) * | 2015-09-28 | 2015-12-16 | 浙江大学 | Adjustable light source device for visible near infrared spectrum detection of internal quality of fruits |
| CN106764497A (en) * | 2017-01-13 | 2017-05-31 | 中国科学院新疆理化技术研究所 | A kind of intelligent multi-wavelength LED array light source |
| CN108535221A (en) * | 2017-03-03 | 2018-09-14 | 广州博鹭腾仪器仪表有限公司 | A kind of new type gel imaging system |
| CN209120509U (en) * | 2018-08-18 | 2019-07-16 | 珠海森龙生物科技有限公司 | Integrated multi-wavelength examines light source |
-
2020
- 2020-09-25 CN CN202011020063.5A patent/CN112268880A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001083090A (en) * | 1999-09-10 | 2001-03-30 | Fuji Photo Film Co Ltd | Excitation light source for micro titer plate |
| US20120298885A1 (en) * | 2011-05-26 | 2012-11-29 | Fujifilm Corporation | Fluorometric assay apparatus and fluorometric assay method |
| CN104655599A (en) * | 2013-11-19 | 2015-05-27 | 郑兆珉 | Fluorescent test paper, fluorescent excitation device and portable fluorescent analysis system |
| CN204556502U (en) * | 2015-04-17 | 2015-08-12 | 上海生物信息技术研究中心 | A kind of fluorescence imaging analysis system |
| CN105158204A (en) * | 2015-09-28 | 2015-12-16 | 浙江大学 | Adjustable light source device for visible near infrared spectrum detection of internal quality of fruits |
| CN106764497A (en) * | 2017-01-13 | 2017-05-31 | 中国科学院新疆理化技术研究所 | A kind of intelligent multi-wavelength LED array light source |
| CN108535221A (en) * | 2017-03-03 | 2018-09-14 | 广州博鹭腾仪器仪表有限公司 | A kind of new type gel imaging system |
| CN209120509U (en) * | 2018-08-18 | 2019-07-16 | 珠海森龙生物科技有限公司 | Integrated multi-wavelength examines light source |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20200056985A1 (en) | Light source module & analytical instrument for analyzing a sample | |
| US20020071124A1 (en) | Device for a quantified determination of the quality of surfaces | |
| US7456955B2 (en) | Spectrophotometer with light emitting diode illuminator | |
| US7649161B2 (en) | Light source utilizing light pipes for optical feedback | |
| KR20130114049A (en) | A light source for lcd back-lit displays | |
| CN101389936A (en) | Color sensor | |
| CN104865688B (en) | The microscope of external fluorescent moieties | |
| CN207424456U (en) | Backlight module and display device | |
| CN201034939Y (en) | Fluorescent powder excited measurement mechanism | |
| CN105938016A (en) | Color measurement apparatus | |
| CN217237745U (en) | Miniature multichannel fluorescence detection system | |
| CN111989521A (en) | Projection status indicator | |
| CN214503374U (en) | Multi-wavelength LED transmission light source fluorescence detection equipment based on PC control | |
| US20100052547A1 (en) | Illumination system | |
| CN215115823U (en) | Multi-wavelength LED transmission light source fluorescence detection equipment | |
| CN112268880A (en) | Multi-wavelength LED transmission light source fluorescence detection equipment and method based on PC control | |
| CN109431439A (en) | A kind of light source feedback device and control method, endoscope | |
| CN201043952Y (en) | Measuring device for fluorescent powder excitation spectrum | |
| CN112264916A (en) | Multi-wavelength LED transmission light source fluorescence detection equipment and application | |
| US20070053058A1 (en) | Lighting assembly for a luminescence analysis apparatus, in particular a fluorescence mrcroscope, and luminescence analysis apparatus equipped with such a lighting assembly | |
| CN214668587U (en) | Ultraviolet LED transmission light source fluorescence detection equipment | |
| CN106772979A (en) | A kind of intelligent multi-wavelength LED fluorescence microscope light source | |
| CN1195217C (en) | Phosphorescence afterglow measuring equipment | |
| CN110609026B (en) | Laser power feedback control up-conversion fluorescence imaging device and imaging method thereof | |
| TWM504953U (en) | A portable spectroscope with multi-bands |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |