CN112263498A - Melanin hair dye and using method thereof - Google Patents
Melanin hair dye and using method thereof Download PDFInfo
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- CN112263498A CN112263498A CN202011184906.5A CN202011184906A CN112263498A CN 112263498 A CN112263498 A CN 112263498A CN 202011184906 A CN202011184906 A CN 202011184906A CN 112263498 A CN112263498 A CN 112263498A
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- hair
- agent
- melanin
- hair dye
- dyeing
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 title claims abstract description 120
- 239000000118 hair dye Substances 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 47
- 238000004043 dyeing Methods 0.000 claims abstract description 36
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 30
- 239000003755 preservative agent Substances 0.000 claims description 30
- 230000002335 preservative effect Effects 0.000 claims description 30
- 210000002752 melanocyte Anatomy 0.000 claims description 20
- 239000008187 granular material Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 229960005070 ascorbic acid Drugs 0.000 claims description 15
- 235000010323 ascorbic acid Nutrition 0.000 claims description 15
- 239000011668 ascorbic acid Substances 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000002562 thickening agent Substances 0.000 claims description 8
- 102000004142 Trypsin Human genes 0.000 claims description 7
- 108090000631 Trypsin Proteins 0.000 claims description 7
- 239000012588 trypsin Substances 0.000 claims description 7
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 6
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 6
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 6
- 229920002907 Guar gum Polymers 0.000 claims description 5
- 230000007910 cell fusion Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 239000000665 guar gum Substances 0.000 claims description 5
- 235000010417 guar gum Nutrition 0.000 claims description 5
- 229960002154 guar gum Drugs 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 5
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 238000010257 thawing Methods 0.000 claims description 5
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 4
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 4
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 235000010413 sodium alginate Nutrition 0.000 claims description 4
- 239000000661 sodium alginate Substances 0.000 claims description 4
- 229940005550 sodium alginate Drugs 0.000 claims description 4
- 108010024636 Glutathione Proteins 0.000 claims description 3
- 229940037003 alum Drugs 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 3
- 229960003180 glutathione Drugs 0.000 claims description 3
- 230000037308 hair color Effects 0.000 claims description 3
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 3
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 3
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 3
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 2
- 108010010803 Gelatin Proteins 0.000 claims description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims description 2
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 2
- 239000001099 ammonium carbonate Substances 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 2
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 230000001079 digestive effect Effects 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 235000019800 disodium phosphate Nutrition 0.000 claims description 2
- 229960002089 ferrous chloride Drugs 0.000 claims description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 229940014259 gelatin Drugs 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 2
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 2
- 239000000230 xanthan gum Substances 0.000 claims description 2
- 235000010493 xanthan gum Nutrition 0.000 claims description 2
- 229920001285 xanthan gum Polymers 0.000 claims description 2
- 229940082509 xanthan gum Drugs 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 239000002537 cosmetic Substances 0.000 abstract description 3
- 239000000049 pigment Substances 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 description 10
- 239000008367 deionised water Substances 0.000 description 9
- 229910021641 deionized water Inorganic materials 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 8
- 229960005323 phenoxyethanol Drugs 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 239000002453 shampoo Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000005562 fading Methods 0.000 description 4
- 239000001024 permanent hair color Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000001678 irradiating effect Effects 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 3
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 3
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 239000003157 biological pigment Substances 0.000 description 2
- 230000009920 chelation Effects 0.000 description 2
- 238000002845 discoloration Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
- FECNOIODIVNEKI-UHFFFAOYSA-N 2-[(2-aminobenzoyl)amino]benzoic acid Chemical class NC1=CC=CC=C1C(=O)NC1=CC=CC=C1C(O)=O FECNOIODIVNEKI-UHFFFAOYSA-N 0.000 description 1
- 206010012434 Dermatitis allergic Diseases 0.000 description 1
- 240000002943 Elettaria cardamomum Species 0.000 description 1
- 206010019030 Hair colour changes Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000005300 cardamomo Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- -1 dye intermediates Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 206010023332 keratitis Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000001026 semi permanent hair color Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/10—Preparations for permanently dyeing the hair
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the field of cosmetics, and particularly relates to a melanin hair dye and a using method thereof. The metal ions in the agent B enter human hair and are firstly complexed with amino acid in the hair, the agent C enters the hair and is complexed with the metal ions to form macromolecular melanin, hair scales are closed, and the pigment is locked inside a hair rod. The hair dye has firm and stable hair dyeing effect, is natural and harmless, can be stored for 3 years under the sealing condition at normal temperature, and has short hair dyeing time and low hair dyeing temperature.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a melanin hair dye and a using method thereof.
Background
Hair dye refers to cosmetic capable of changing hair color and having special application. The hair dyes that have been most used for a long time are permanent and semi-permanent hair dyes. However, as an oxidation dye which is a main component of the oxidation hair dye, a conventional permanent hair dye is also called an oxidation hair dye. The specific dyeing mechanism of the hair dye is that oxidation dye intermediate with low relative molecular mass and other components penetrate into the hair, undergo oxidation polymerization in the hair to generate macromolecular dye and are locked in the hair. Oxidative hair dyes are mostly composed of 3 reactive compounds, namely, dye intermediates, couplers (also called couplers) and oxidizing agents, and particularly p-phenylenediamine has been found to be an allergic substance which causes allergic dermatitis and is banned by some european countries. Recent scientific research also shows that phenylenediamine and some derivatives thereof have accumulative property, rust degeneration and mutation property, and can cause keratitis, nephritis, leukemia and the like. Meanwhile, most of the permanent hair dyes in the current market are two-agent hair dyes, and the permanent hair dyes need to be blended before hair dyeing, so that the hair dyeing process is complicated.
The plant type hair dye uses one plant extract or a mixture of a plurality of plants as a raw material of the hair dye, wherein the mixture of the plurality of plant extracts is used for preparing most of the hair dyes, the extraction process is complicated, and the plurality of hair dyes are prepared only primarily and no products are produced. It is reported that the domestic health department has not approved the plant hair dyes made of plant extracts completely, and most of the commercially available 'pure plant hair dyes' are gimmicks made of some ingredients containing plants, wherein some chemical ingredients such as PPD are mixed.
Melanin is divided into synthetic melanin and natural melanin, and natural melanin refers to a natural biological pigment extracted from plants, animals and microorganisms, and has a wide variety of sources, and is the most extensive pigment in the known biological pigments at present. Melanin from natural sources is insoluble in acid and water, but has greater solubility in lye where p H is greater than 8.5. The melanin is insoluble in ethanol, methanol, acetone, ether and other common organic solvents, the chelation capacity of the melanin to the metal ions with the valence of 2 and 3 is very strong, and at present, a plurality of black hair dyes achieve the purpose of coloring by utilizing the chelation of the melanin and the metal ions.
The existing hair dye has unstable dyeing color and is easy to have the problems of hair color change or fading in the hair washing process.
Disclosure of Invention
The invention mainly aims to provide a melanin hair dye, which is a novel three-agent type hair dye prepared by taking melanin naturally produced by culturing melanocytes as a raw material of the hair dye. Under certain conditions, melanin can be complexed with metal ions to form a macromolecular compound, but the diameter of the melanin molecule is large, so that the melanin molecule is not beneficial to permeating into hair. Therefore, the agent A is used as a hair pretreatment agent, and the penetrant is used to open the disulfide bonds in the hair, thereby facilitating the penetration of the following metal ions and coloring agents into the hair. The metal ions in the agent B enter human hair and are firstly complexed with amino acid in the hair, the agent C enters the hair and is complexed with the metal ions to form macromolecular melanin, hair scales are closed, and the pigment is locked inside a hair rod.
In order to achieve the purpose, the invention adopts the following technical scheme:
a melanin hair dye comprises a combination of agent A, agent B and agent C; the agent A is prepared from the following components in percentage by mass: 1-20% of penetrating agent, 0.1-2% of ascorbic acid, 0.1-2% of thickening agent, 0.01-10% of alkaline agent, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the agent B is prepared from the following components in percentage by mass: 1-10% of metal ions, 0.1-2% of ascorbic acid, 0.1-2% of thickening agent, 0.01-1% of citric acid, 0.01-10% of alkali, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the agent C is prepared from the following components in percentage by mass: 0.1-2% of ascorbic acid, 0.1-2% of melanin granules, 0.1-2% of thickening agent, 0.01-10% of alkaline agent, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the melanin granules are made from primary human melanocytes, trypsin and citric acid.
Further, the preparation method of the melanin granules comprises the following steps:
s1, melanocyte culture: culturing primary human melanocyte until cell fusion degree reaches 90%, digesting cells with trypsin digestive juice with mass fraction of 0.25%, centrifuging at 800rpm/min for 5min, suspending cells with PBS, and centrifuging at 50-200mJ/cm2The suspension was irradiated at a UVB dose of (1: 2-1: 3 for 24-48h to obtain melanocytes for later use;
s2, obtaining melanin granules: repeatedly freezing and thawing the melanocyte obtained in S1 for 3-4 times, collecting liquid, filtering, acidifying with citric acid, centrifuging, and obtaining precipitate, namely melanin granules.
Further, the penetrating agent is at least one selected from the following: l-cysteine and glutathione.
Further, the metal ion is at least one selected from the group consisting of: ferric chloride, magnesium chloride, zinc sulfate, copper sulfate, ferrous chloride, aluminum chloride, alum, and copper nitrate.
Further, the thickener is at least one selected from the group consisting of: hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC), hydroxypropyl methyl cellulose (HPMC), xanthan gum, guar gum, sodium alginate, sodium hyaluronate, gelatin, and sodium alginate.
Further, the alkaline agent is at least one selected from the following: ammonia, ammonium bicarbonate, sodium hydrogen phosphate, sodium hydroxide, potassium hydroxide, ethanolamine, triethanolamine, arginine, and lysine.
Further, the use method of the melanin hair dye comprises the following steps: uniformly coating a certain amount of hair dye A on hair, sealing with a preservative film, and dyeing at 25-40 deg.C for 20-50 min; then uniformly coating a certain amount of hair dye B on the hair, sealing with a preservative film, and dyeing at 25-40 deg.C for 20-50 min; then uniformly coating a certain amount of hair dye C on hair, sealing with preservative film, and dyeing at 25-40 deg.C for 20-50min
Compared with the prior art, the invention has the following technical effects:
the hair dye has firm and stable hair dyeing effect, the hair color is close to the hair dyeing result of the hair dye sold in the market, the beauty concept of the public is met, the hair dye is natural and harmless, the hair dye can be stored for 3 years under the sealing condition at normal temperature, the hair dyeing time is short, and the hair dyeing temperature is low.
Drawings
Fig. 1 is a graph showing the comparison of the effects of natural human black hair before dyeing, and after dyeing using the melanin hairdye of the present invention, wherein a is before dyeing, b is natural human black hair, and c is after dyeing.
Fig. 2 shows the results before and after dyeing with a commercial hair dye, wherein a is before dyeing and B is after dyeing.
Detailed Description
The invention is described in detail below with reference to the following figures and specific examples, which should not be construed as limiting the invention. The technical means used in the following examples are conventional means well known to those skilled in the art, and materials, reagents and the like used in the following examples can be commercially available unless otherwise specified.
Example 1
A melanin hair dye comprises the following preparation steps:
(1) culturing melanocytes: culturing primary human melanocyte (P3) obtained by normal separation with M254 culture medium, digesting the cells (0.25% trypsin) when the cell fusion degree reaches 90%, centrifuging (800rpm/min, 5min), suspending the cells with PBS, irradiating the suspension with UVB dose of 100mJ/cm2, continuously culturing at the ratio of 1:2 after centrifugation for 24h, and placing in a refrigerator at-80 ℃ for later use.
(2) Obtaining of melanin granules: and (2) repeatedly freezing and thawing the melanocytes obtained in the step (1) for 3-4 times, collecting liquid, filtering, acidifying by adding citric acid, and centrifuging to obtain precipitates, namely melanin granules. (3) Preparing a melanin hair dye:
the hair dye comprises three formulations, namely, formulation A: adding 5 parts of L-cysteine, 0.6 part of ascorbic acid (VC), 1 part of hydroxyethyl cellulose, 0.3 part of preservative, 4 parts of ammonia water and the balance of deionized water in sequence by weight part of 100 parts, and uniformly mixing.
And (2) agent B: adding 4 parts of alum, 0.5 part of ascorbic acid (VC), 1 part of hydroxyethyl cellulose, 0.3 part of preservative (phenoxyethanol), 0.01 part of citric acid and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
C, agent C: adding 0.5 part of ascorbic acid (VC), 2 parts of melanin granules, 1 part of hydroxyethyl cellulose, 0.3 part of preservative (phenoxyethanol), 6 parts of ammonia water and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
The use method of the melanin hair dye comprises the following steps: firstly, uniformly coating the agent A on hair, wrapping the hair with a preservative film, and preserving the temperature for 30min at 35 ℃; then smearing the agent B on hair, wrapping with preservative film, and keeping the temperature at 40 deg.C for 30 min; finally, the agent C is smeared on the hair, wrapped by a preservative film and kept at the temperature of 35 ℃ for 30 min.
Example 2
A melanin hair dye comprises the following preparation steps:
(1) culturing melanocytes: culturing primary human melanocyte (P4) obtained by normal separation with M254 culture medium, digesting the cells (0.25% trypsin) when the cell fusion degree reaches 90%, centrifuging (800rpm/min, 5min), suspending the cells with PBS, irradiating the suspension with UVB dose of 150mJ/cm2, continuously culturing at a ratio of 1:2 after centrifugation for 36h, and placing in a refrigerator at-80 ℃ for later use.
(2) Obtaining of melanin granules: and (2) repeatedly freezing and thawing the melanocytes obtained in the step (1) for 4 times, collecting liquid, filtering, acidifying by adding citric acid, and centrifuging to obtain precipitates, namely melanin granules.
(3) Preparing a melanin hair dye:
the hair dye comprises three formulations, namely, formulation A: adding 4 parts of L-cysteine, 0.8 part of ascorbic acid (VC), 0.8 part of guar gum, 2 parts of preservative (phenoxyethanol), 3 parts of essence, 0.2 part of sodium hydroxide and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
And (2) agent B: adding 4 parts of ferric chloride, 0.3 part of ascorbic acid (VC), 0.8 part of guar gum, 2 parts of preservative (phenoxyethanol), 3 parts of essence, 0.02 part of citric acid and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
C, agent C: adding 0.6 part of ascorbic acid (VC), 1 part of melanin particles, 0.8 part of guar gum, 2 parts of preservative (phenoxyethanol), 3 parts of essence, 0.3 part of sodium hydroxide and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
The use method of the melanin hair dye comprises the following steps: firstly, uniformly coating the agent A on hair, wrapping the hair with a preservative film, and preserving the temperature at 33 ℃ for 30 min; then smearing the agent B on hair, wrapping with preservative film, and keeping the temperature at 30 ℃ for 30 min; finally, the agent C is smeared on the hair, wrapped by a preservative film and kept at the temperature of 35 ℃ for 30 min.
Example 3
A melanin hair dye comprises the following preparation steps:
(1) culturing melanocytes: culturing primary human melanocyte (p4) obtained by normal separation with M254 culture medium, digesting the cells (0.25% trypsin) when the cell fusion degree reaches 90%, centrifuging (800rpm/min, 5min), suspending the cells with PBS, irradiating the suspension with UVB dose of 150mJ/cm2, continuously culturing at a ratio of 1:3 after centrifugation for 48h, and placing in a refrigerator at-80 ℃ for later use.
(2) Obtaining of melanin granules: and (2) repeatedly freezing and thawing the melanocytes obtained in the step (1) for 3 times, collecting liquid, filtering, acidifying by adding citric acid, and centrifuging to obtain precipitates, namely melanin granules.
(3) Preparing a melanin hair dye:
the hair dye comprises three formulations, namely, formulation A: adding 4 parts of glutathione, 0.4 part of ascorbic acid (VC), 0.8 part of sodium carboxymethylcellulose, 1.5 parts of preservative (phenoxyethanol), 2 parts of essence, 3 parts of ammonia water and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
And (2) agent B: adding 4 parts of ferric chloride, 0.7 part of ascorbic acid (VC), 0.8 part of sodium carboxymethylcellulose, 1.5 parts of preservative (phenoxyethanol), 2 parts of essence, 0.02 part of citric acid and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
C, agent C: adding 0.6 part of ascorbic acid (VC), 2 parts of melanin, 0.8 part of sodium carboxymethylcellulose, 1.5 parts of preservative (phenoxyethanol), 2 parts of essence, 5 parts of ammonia water and the balance of deionized water in sequence by weight of 100 parts, and uniformly mixing.
The use method of the melanin hair dye comprises the following steps: firstly, uniformly coating the agent A on hair, wrapping the hair with a preservative film, and preserving the temperature for 30min at 35 ℃; then smearing the agent B on hair, wrapping with preservative film, and keeping the temperature at 30 ℃ for 30 min; finally, the agent C is smeared on the hair, wrapped by a preservative film and kept at the temperature of 25 ℃ for 50 min.
Hair dyeing and evaluation
(1) Compared with the hair dye sold in the market, the hair dye of the invention
Washing equivalent white hair with shampoo, mixing two commercially available hair dyes, namely, the Cardamom pleasant hair dye cream glittering pearl black A agent and the B agent uniformly, spreading the mixture on the hair, and washing off the loose hair with clear water after 40 min.
An equal amount of white hair was taken, and after being washed with shampoo, the hair dye of the present invention was used to dye hair according to the procedure of example 3.
Then, white hair, natural black hair of human, the effect dyed with the melanin hair dye prepared in example 3, and the effect dyed with a commercially available hair dye were observed under a hair mirror.
The results are shown in figure 1, and it can be seen from the results that the product has ideal hair dyeing effect on white hair, has small damage to the hair, and the dyed hair is closer to natural black hair of a human body.
(2) Hair dyeing and evaluation
(1) Dyeing the hair: the melanin hairdyes of examples 1 to 3 were applied to commercially available hair strands, respectively, according to the procedure of example 3, and the hair was dyed. Dyeing and dyeing of commercially available Hair dyes, comparative example 1 was followed by washing with commercially available 2-in-1 shampoo (Anlishing two-in-one shampoo) and drying naturally to give four "freshly dyed" hair strands.
The above four "just dyed" hair strand colors were tested using a YS3010 spectrocolorimeter manufactured by Chongqing Chonggao laboratory instruments ltd. The results of 10 measurements were averaged out, and the results are shown in Table 2.
(2) Evaluation of degree of discoloration: the hair tresses just dyed were washed 10 times and 20 times with commercially available 2 in 1 shampoos, respectively, and then naturally dried to give "wash 10 times" and "wash 20 times". The color difference of hair tresses "washed 10 times", "washed 20 times" and "immediately after dyeing" was evaluated by visual observation of 20 participants according to the following evaluation criteria, and the results were averaged as shown in table 2.
5 evaluation criteria for discoloration:
5-little color difference immediately after dyeing and after fading;
4, the color difference between the dyed cloth and the faded cloth is small;
3-moderate color difference immediately after dyeing and after fading;
2-the color difference between the dyed and faded is large;
the color difference immediately after 1-dyeing and after fading was very large.
TABLE 1 evaluation results of hair dyeing
As can be seen from the results in Table 1, the melanin hair dye of the present invention has a superior dyeing effect and is excellent in washing fastness, and can be used as a semi-permanent or permanent type hair dye.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. A melanin hair dye is characterized by comprising a combination of an agent A, an agent B and an agent C;
the agent A is prepared from the following components in percentage by mass: 1-20% of penetrating agent, 0.1-2% of ascorbic acid, 0.1-2% of thickening agent, 0.01-10% of alkaline agent, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the agent B is prepared from the following components in percentage by mass: 1-10% of metal ions, 0.1-2% of ascorbic acid, 0.1-2% of thickening agent, 0.01-1% of citric acid, 0.01-10% of alkali, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the agent C is prepared from the following components in percentage by mass: 0.1-2% of ascorbic acid, 0.1-2% of melanin granules, 0.1-2% of thickening agent, 0.01-10% of alkaline agent, 0.3-2% of preservative, 0.0-3% of essence and the balance of water;
the melanin granules are made from primary human melanocytes, trypsin and citric acid.
2. The melanin hair dye according to claim 1, wherein the melanin granules are prepared by a method comprising:
s1, melanocyte culture: culturing primary human melanocyte until cell fusion degree reaches 90%, digesting cells with trypsin digestive juice with mass fraction of 0.25%, centrifuging at 800rpm/min for 5min, suspending cells with PBS, and centrifuging at 50-200mJ/cm2The suspension was irradiated at a UVB dose of (1: 2-1: 3 for 24-48h to obtain melanocytes for later use;
s2, obtaining melanin granules: repeatedly freezing and thawing the melanocyte obtained in S1 for 3-4 times, collecting liquid, filtering, acidifying with citric acid, centrifuging, and obtaining precipitate, namely melanin granules.
3. The melanin hair dye according to claim 1, wherein the penetrating agent is at least one selected from the group consisting of: l-cysteine and glutathione.
4. The melanin hair dye according to claim 1, wherein the metal ions are at least one selected from the group consisting of: ferric chloride, magnesium chloride, zinc sulfate, copper sulfate, ferrous chloride, aluminum chloride, alum, and copper nitrate.
5. The melanin hair dye according to claim 1, wherein the thickener is at least one selected from the group consisting of: hydroxyethyl cellulose, carboxymethyl cellulose, hydroxypropyl methyl cellulose, xanthan gum, guar gum, sodium alginate, sodium hyaluronate, gelatin, and sodium alginate.
6. The melanin hair dye according to claim 1, wherein the alkaline agent is at least one selected from the group consisting of: ammonia, ammonium bicarbonate, sodium hydrogen phosphate, sodium hydroxide, potassium hydroxide, ethanolamine, triethanolamine, arginine, and lysine.
7. The method of using a melanin hair coloring agent of claim 1, comprising the steps of: uniformly coating the hair dye A on hair, sealing with preservative film, and dyeing at 25-40 deg.C for 20-50 min; uniformly coating the hair dye B on hair, sealing with a preservative film, and dyeing at 25-40 deg.C for 20-50 min; then uniformly coating the hair dye C on the hair, sealing with a preservative film, and dyeing at 25-40 deg.C for 20-50 min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114099359A (en) * | 2021-12-02 | 2022-03-01 | 台州学院 | dopamine-Fe 2+ colorful hair dye and hair dyeing method |
| CN114831907A (en) * | 2022-06-22 | 2022-08-02 | 华南农业大学 | Boletus melanin hair dye and preparation method and use method thereof |
| CN115414304A (en) * | 2022-07-01 | 2022-12-02 | 佛山嘿黑科技有限公司 | Bi-component hair dye composition and preparation method thereof |
| CN115517989A (en) * | 2022-01-25 | 2022-12-27 | 西南医科大学 | A four-component ammonia-free and non-toxic permanent hair dyeing composition and its preparation method |
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2020
- 2020-10-29 CN CN202011184906.5A patent/CN112263498A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114099359A (en) * | 2021-12-02 | 2022-03-01 | 台州学院 | dopamine-Fe 2+ colorful hair dye and hair dyeing method |
| CN115517989A (en) * | 2022-01-25 | 2022-12-27 | 西南医科大学 | A four-component ammonia-free and non-toxic permanent hair dyeing composition and its preparation method |
| CN114831907A (en) * | 2022-06-22 | 2022-08-02 | 华南农业大学 | Boletus melanin hair dye and preparation method and use method thereof |
| CN115414304A (en) * | 2022-07-01 | 2022-12-02 | 佛山嘿黑科技有限公司 | Bi-component hair dye composition and preparation method thereof |
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