CN112261951A - 包含合成嵌合痘苗病毒的干细胞及其使用方法 - Google Patents

包含合成嵌合痘苗病毒的干细胞及其使用方法 Download PDF

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CN112261951A
CN112261951A CN201980029672.6A CN201980029672A CN112261951A CN 112261951 A CN112261951 A CN 112261951A CN 201980029672 A CN201980029672 A CN 201980029672A CN 112261951 A CN112261951 A CN 112261951A
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virus
cells
stem cell
vacv
population
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S·莱德尔曼
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Tonix Pharma Holdings Ltd
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CN201980029672.6A 2018-05-02 2019-05-02 包含合成嵌合痘苗病毒的干细胞及其使用方法 Pending CN112261951A (zh)

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US201862666013P 2018-05-02 2018-05-02
US62/666,013 2018-05-02
PCT/US2019/030488 WO2019213453A1 (en) 2018-05-02 2019-05-02 Stem cells comprising synthetic chimeric vaccinia virus and methods of using them

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US (1) US20210236619A1 (https=)
EP (1) EP3787681A4 (https=)
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AR (1) AR115070A1 (https=)
AU (1) AU2019262150A1 (https=)
CA (1) CA3098145A1 (https=)
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JP2019535311A (ja) 2016-11-02 2019-12-12 デイビッド エバンス, 合成キメラポックスウイルス
EP3788142A4 (en) * 2018-05-02 2022-04-27 Tonix Pharma Holdings Limited SYNTHETIC CHIMERIC VACCINE VIRUS

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703389A (zh) * 2007-11-19 2012-10-03 特朗斯吉有限公司 痘病毒溶瘤载体
WO2013083254A1 (en) * 2011-12-09 2013-06-13 Bavarian Nordic A/S Poxvirus vector for the expression of bacterial antigens linked to tetanus toxin fragment c
CN105535993A (zh) * 2015-12-25 2016-05-04 浙江省人民医院 重组溶瘤痘苗病毒在治疗胃癌中的用途
WO2017027757A2 (en) * 2015-08-11 2017-02-16 StemImmune, Incorporated Smallpox vaccine for cancer treatment
CN107303302A (zh) * 2009-09-14 2017-10-31 希拉金股份有限公司 溶瘤牛痘病毒组合癌症疗法
US20180078591A1 (en) * 2015-04-17 2018-03-22 Memorial Sloan Kettering Cancer Center Use of mva or mvadeltae3l as immunotherapeutic agents against solid tumors

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2709292A1 (en) * 2009-07-10 2011-01-10 The Governors Of The University Of Alberta Oncolytic viruses and methods for treating neoplastic disorders
WO2014160475A1 (en) * 2013-03-13 2014-10-02 Aboody Karen S Tropic cell based virotherapy for the treatment of cancer
ES2702618B2 (es) * 2017-09-04 2019-10-31 Inst De Salud Carlos Iii Producto de combinación que comprende una célula madre mesenquimatosa modificada y una sustancia antigénica.
WO2019213173A1 (en) * 2018-04-30 2019-11-07 City Of Hope Neural stem cell compositions including chimeric poxviruses for cancer treatment

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703389A (zh) * 2007-11-19 2012-10-03 特朗斯吉有限公司 痘病毒溶瘤载体
CN107303302A (zh) * 2009-09-14 2017-10-31 希拉金股份有限公司 溶瘤牛痘病毒组合癌症疗法
WO2013083254A1 (en) * 2011-12-09 2013-06-13 Bavarian Nordic A/S Poxvirus vector for the expression of bacterial antigens linked to tetanus toxin fragment c
US20180078591A1 (en) * 2015-04-17 2018-03-22 Memorial Sloan Kettering Cancer Center Use of mva or mvadeltae3l as immunotherapeutic agents against solid tumors
WO2017027757A2 (en) * 2015-08-11 2017-02-16 StemImmune, Incorporated Smallpox vaccine for cancer treatment
CN105535993A (zh) * 2015-12-25 2016-05-04 浙江省人民医院 重组溶瘤痘苗病毒在治疗胃癌中的用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RYAN S. NOYCE等: "Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments", 《PLOS ONE》, vol. 13, no. 1, 19 January 2018 (2018-01-19), pages 1 - 16, XP055648704, DOI: 10.1371/journal.pone.0188453 *

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