CN112251393B

CN112251393B - Cell separation device

Info

Publication number: CN112251393B
Application number: CN202011116316.9A
Authority: CN
Inventors: 毛静涛
Original assignee: Beijing Dragon Midas Science And Technology Development Co ltd
Current assignee: Beijing Dragon Midas Science And Technology Development Co ltd
Priority date: 2020-10-19
Filing date: 2020-10-19
Publication date: 2022-05-10
Anticipated expiration: 2040-10-19
Also published as: CN112251393A

Abstract

The invention provides a cell separation method, which comprises the following steps: fixing the antibody; preparing a device; cell adsorption; the cells were eluted. The invention also provides a cell separation device which comprises a shell and an adsorption assembly, wherein a cell pool and an elution pool are arranged in the shell, a pool wall is formed between the cell pool and the elution pool, a notch is formed in the pool wall, a rotating shaft is arranged in the pool wall at the notch, the adsorption assembly comprises an adsorption device, the adsorption device is positioned at the notch and matched with the notch, the adsorption device is connected with the rotating shaft on the pool wall, the exchange of partial structures entering the cell pool and the elution pool can be realized, and a load groove is formed in the adsorption device and used for loading antibodies. The cell separation method and the cell separation device can be used for simultaneously adsorbing and eluting cells, so that the time is saved, and the efficiency is improved; the eluted adsorption device is moved into the cell pool again to adsorb the cells again, and the method can perform cyclic adsorption and elution to improve the cell separation ratio.

Description

Cell separation device

Technical Field

The invention relates to the field of cell culture, in particular to a cell separation device.

Background

In the prior art, cells are often required to be separated for subsequent experiments; at present, various laboratories have multiple physical methods such as density gradient centrifugation, flow cytometry and the like, but by adopting the means, the proportion of cell separation is generally not more than 80%, and meanwhile, the methods have various limitations. The existing technology for separating cells by using antigen-antibody reaction is complicated in the target cell transfer process, and the time for cell adsorption and elution is long.

Therefore, there is a need in the art for a cell separation device.

The invention is provided in view of the above.

Disclosure of Invention

The present invention is directed to a cell separation device to solve at least one of the above problems.

Specifically, the invention provides a cell separation method, which comprises the following steps:

fixing the antibody, determining the antigen on the surface of the target cell, preparing a specific antibody of the antigen, and fixing the antibody on the surface of the adsorption device;

preparing a device, mounting the adsorption device on the corresponding cell pool and penetrating through the wall of the cell pool, wherein part of the adsorption device enters the cell pool, and part of the adsorption device is outside the cell pool;

cell adsorption, namely adding a cell suspension containing target cells into the cell pool, controlling the adsorption device to move part of the structure entering the cell pool out of the cell pool and move part of the structure outside the cell pool into the cell pool after the adsorption device enters part of the structure in the cell pool to adsorb the cells, and carrying out next round of adsorption;

and (3) cell elution, namely eluting the target cells on the adsorption device outside the cell pool and collecting the eluent.

By adopting the technical scheme, the target cells are the cells to be separated, and the method can be used for adsorbing and eluting the cells simultaneously, so that the time is saved, and the efficiency is improved; the eluted adsorption device is moved into the cell pool again to adsorb the cells again, and the method can perform cyclic adsorption and elution to improve the cell separation ratio.

Preferably, the antibody is covalently coupled to the adsorption means.

By adopting the technical scheme, the antibody is not easy to elute, and is convenient to repeatedly adsorb and elute.

The invention provides a cell separation device, which comprises a shell and an adsorption assembly, wherein a cell pool and an elution pool are arranged in the shell, a pool wall is formed between the cell pool and the elution pool, a notch is formed in the pool wall, a rotating shaft is arranged in the pool wall at the notch, the adsorption assembly comprises an adsorption device, the adsorption device is positioned at the notch and matched with the notch, the adsorption device is connected with the rotating shaft on the pool wall, the exchange of partial structures entering the cell pool and the elution pool can be realized, and a load groove is formed in the adsorption device and used for loading an antibody.

In a specific implementation process, the notch can be semi-surrounded or fully surrounded, the rotating shaft can be horizontally or vertically arranged, the adsorption device can be roughly cylindrical, spherical and the like, and can also be any three-dimensional shape obtained by rotation, the adsorption device realizes the exchange of partial structures of the cell pool and the elution pool by rotation, and in the rotation exchange process, the adsorption device is roughly matched with the section of the notch; the rotary exchange process can be 360-degree rotation or reciprocating rotation close to 180 degrees; the loading groove is used for loading the antibody and can carry out immobilization in a physical adsorption or covalent coupling mode.

By adopting the technical scheme, the adsorption of the target cells in the cell pool by utilizing an antigen-antibody reaction is realized through the exchange of the adsorption device on the exposed structures in the cell pool and the elution pool, and the target cells are eluted in the elution pool on the other side so as to improve the efficiency of separating the cells, and the adsorption can be repeated, so that the recovery rate of the target cells is improved compared with single adsorption; the load groove is a target cell attachment space, and the target cells are protected from being rubbed and falling off by the pool wall in the rotating process.

Further, the adsorption device is detachably connected with the rotating shaft.

In the specific implementation process, the adsorption device can be provided with holes and grooves which are matched with the rotating shaft and can be connected in a sleeving, screwing and other modes.

By adopting the technical scheme, the adsorption device can be detached from the shell, so that the adsorption device is convenient to load antibodies and also convenient to replace the adsorption device, and cells of different types can be adsorbed.

Furthermore, a transmission hole is formed in the adsorption device, one end of the rotating shaft penetrates through the transmission hole and is matched with the transmission hole, and the other end of the rotating shaft penetrates through the pool wall and is provided with a rotating piece.

In an embodiment, the rotating member may be a knob or a lever handle.

By adopting the technical scheme, the rotating piece rotates the rotating shaft, so that the adsorbing device is driven to rotate, the adsorbing device can rotate without being directly contacted, and pollution and cell shedding are prevented.

Furthermore, a limiting groove or a limiting boss is arranged on the rotating shaft.

In the specific implementation process, the rotating shaft is matched with the transmission hole, the rotating shaft is provided with a limiting groove or a limiting boss, the transmission hole is correspondingly provided with the limiting boss or the limiting groove, the rotating shaft and the transmission hole can be provided with the limiting groove, and then the rotating shaft and the transmission hole are connected through keys.

By adopting the technical scheme, the limiting groove or the limiting boss is convenient for transmitting rotary motion.

Further, a plurality of notches and adsorption devices on the pool wall can be arranged.

By adopting the technical scheme, the adsorption area can be increased, and the separation efficiency is improved.

Further, one or two separating bosses are arranged on the adsorption device.

By adopting the technical scheme, the separation lug boss is convenient for distinguishing the exchange parts at two sides, and also has the function of limiting, so that excessive rotation is prevented.

Furthermore, a leakage pool is arranged at the bottom of the shell, a leakage channel is arranged on the pool wall, and the leakage channel is communicated with the notch and the leakage pool.

By adopting the technical scheme, a small amount of liquid can be remained in the load groove in the rotation process of the adsorption device, and the liquid is guided into the liquid leakage pool by the liquid leakage channel, so that the liquid in the cell pool and the liquid in the elution pool are prevented from being mixed.

Preferably, the liquid leakage pool is detachably connected with the bottom of the shell.

In a specific implementation process, the leakage pool can be connected with the bottom of the shell in a sliding mode through a sliding groove or in a threaded mode.

By adopting the technical scheme, the liquid in the leakage pool can be poured conveniently and the leakage pool can be cleaned conveniently.

Further, the adsorption device is provided with a smooth part, and the smooth part is not provided with the load groove.

Preferably, the smooth part is provided with an elastic pad, and the elastic pad is made of elastic materials, such as polyphenyl ether, natural rubber and the like.

By adopting the technical scheme, in the adsorption and elution processes, the adsorption device is in a static state, and at the moment, the smooth part is in contact with the notch, so that the tightness of the contact part is improved.

Preferably, the notch is in a semi-surrounding shape, and the rotating shaft is horizontally arranged.

In a specific implementation process, when the separation lug boss is positioned at the top of the notch, the smooth part is positioned at the contact part with the notch.

By adopting the technical scheme, the separation lug boss is convenient to observe through the upper part of the notch, and two exchange parts are convenient to distinguish, so that the exchange is complete.

Further, still be provided with the lid on the casing, the lid with the casing is articulated or detachable connects.

By adopting the technical scheme, the cover body can rotate or separate relative to the shell body, so that the opening is realized, and the checking and the operation are convenient; the cover body prevents the liquid in the cell pool and the elution pool from being polluted or poured during the transfer process.

Preferably, the cover body is connected with the shell in a sliding mode, and the shell is provided with a convex rib.

By adopting the technical scheme, the convex ribs are convenient for the cover body to apply force to slide, and the cover body is convenient to open and close.

Further, when the cover body is in a closed state, the top surface of the separation boss is matched with the bottom surface of the cover body, and the rotation of the adsorption device is limited.

In a specific implementation process, the bottom surface of the cover body and the top surface of the separation boss can be both planes and can contact with each other to limit rotation, and the bottom surface of the cover body can be provided with a groove matched with the top of the separation boss to limit rotation.

By adopting the technical scheme, when the cover body is closed, the adsorption device is fixed, and the two exchange parts are prevented from rotating in the transfer process to influence the adsorption and elution effects.

In conclusion, the invention has the following beneficial effects:

1. through the exchange of the bare part structures of the adsorption device in the cell pool and the elution pool, the purpose of adsorbing the target cells by using antigen-antibody reaction in the cell pool is realized, and meanwhile, the target cells are eluted in the elution pool at the other side, so that the efficiency of separating the cells is improved;

2. the adsorption device is used for circularly reciprocating and repeatedly adsorbing, so that the recovery rate of target cells is improved compared with single adsorption;

3. the target cells are protected from being rubbed and falling off by the pool wall in the rotating process by taking the load groove as a target cell attachment space;

4. the rotating shaft is rotated through the rotating piece, so that the adsorption device is driven to rotate, and the adsorption device can be rotated without directly contacting with the adsorption device, so that pollution and cell shedding are prevented;

5. the adsorption device can be detached from the shell, so that the adsorption device is convenient to load antibodies, and the adsorption device is also convenient to replace so as to adsorb different types of cells;

6. the cover body can rotate or separate relative to the shell body, so that the cover body can be opened, and the checking and the operation are convenient; the cover body prevents the liquid in the cell pool and the elution pool from being polluted or poured during the transfer process;

7. the cover body can also be matched with the separation lug boss to prevent the adsorption device from rotating to influence cell adsorption and elution.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of one embodiment of the cell separation method of the present invention;

FIG. 2 is a perspective view of one embodiment of the cell separation device of the present invention;

FIG. 3 is a top view of one embodiment of the cell separation device of the present invention;

FIG. 4 is a cross-sectional view taken along A-A of FIG. 3;

FIG. 5 is a top view of one embodiment of a sorption assembly of the present invention;

description of the reference numerals

The technical scheme of the invention can be more clearly understood and explained by combining the embodiment of the invention through the reference sign description.

1. A housing; 11. a cell pool; 12. an elution pool; 13. a tank wall; 131. a leakage path; 14. a liquid leakage pool; 15. a cover body; 2. an adsorption component; 21. an adsorption device; 211. a load cell; 212. a drive bore; 213. separating the bosses; 214. a smooth portion; 22. a rotating shaft; 221. a limiting groove; 23. and a rotating member.

Detailed Description

Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the invention, as detailed in the appended claims.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.

The present invention will be described in detail below by way of examples.

Specifically, referring to fig. 1, the present invention provides a cell separation method comprising the steps of:

s100, fixing an antibody, determining an antigen on the surface of a target cell, preparing a specific antibody of the antigen, and fixing the antibody on the surface of an adsorption device 21;

in the specific implementation process, the adsorption device 21 may be made of polystyrene, and the specific antibody is diluted to a proper concentration by the coating solution and then added into the adsorption device 21, wherein the proper concentration may be 0.1-10 μ g/ml; the coating buffer can be 0.05M carbonate buffer at pH 9.6; after incubation for 0.5-2h, the mixture is placed in a refrigerator at 4 ℃ for 12-24 h. Specifically, theThe preparation method of the carbonate buffer solution can be as follows: weighing NaCO₃ 1.59g，NaHCO₃2.93g, deionized water was added to 1L and dissolved sufficiently. The process of fixing the antibody on the surface of the adsorption device 21 may also adopt an EDC/NHS cross-linking method.

S200, preparing a device, namely installing the adsorption devices 21 on the corresponding cell pools 11 and penetrating through the walls 13 of the cell pools 11, wherein the adsorption devices 21 partially enter the cell pools 11 and partially are outside the cell pools 11;

s300, cell adsorption, namely adding a cell suspension containing target cells into the cell pool 11, controlling the adsorption device 21 to move part of the structure entering the cell pool 11 out of the cell pool 11 and move part of the structure outside the cell pool 11 into the cell pool 11 after the adsorption device 21 enters part of the structure in the cell pool 11 to adsorb the cells, and performing next round of adsorption;

s400, cell elution, namely eluting target cells on the adsorption device 21 outside the cell pool 11 and collecting eluent.

By adopting the technical scheme, the target cells are the cells to be separated, and the method can be used for adsorbing and eluting the cells simultaneously, so that the time is saved, and the efficiency is improved; the adsorption device 21 after elution is moved into the cell pool 11 again to adsorb the cells again, and the method can perform cyclic adsorption and elution to improve the cell separation ratio.

In a preferred embodiment of the invention, the antibody is covalently coupled to the adsorption means 21.

Referring to fig. 2 and 4, in another aspect of the present invention, a cell separation device is provided, which includes a housing 1 and an adsorption assembly 2, a cell pool 11 and an elution pool 12 are disposed in the housing 1, a pool wall 13 is formed between the cell pool 11 and the elution pool 12, the pool wall 13 is provided with a gap, a rotating shaft 22 is disposed in the pool wall 13 at the gap, the adsorption assembly 2 includes an adsorption device 21, the adsorption device 21 is disposed at the gap and is matched with the gap, the adsorption device 21 is connected to the rotating shaft 22 on the pool wall 13, so as to exchange a partial structure entering the cell pool 11 with a partial structure entering the elution pool 12, a loading slot 211 is disposed on the adsorption device 21, and the loading slot 211 is used for loading an antibody.

In a specific implementation process, the notch can be semi-surrounded or fully surrounded, the rotating shaft 22 can be horizontally or vertically arranged, the general shape of the adsorption device 21 can be cylindrical, spherical and the like, and can also be any three-dimensional shape obtained by rotation, the adsorption device 21 realizes the exchange of partial structures between the cell pool 11 and the elution pool 12 through rotation, and in the rotation exchange process, the section of the adsorption device 21 and the section of the notch are approximately matched; the rotary exchange process can be 360-degree rotation or reciprocating rotation close to 180 degrees; the loading groove 211 is used for loading antibody, and can be immobilized by physical adsorption or covalent coupling.

In a preferred embodiment of the present invention, the adsorption device 21 is cylindrical, and the center of the circle is connected to the rotation shaft 22, so as to realize rotation; the load slot may be provided at upper and lower bottom surfaces, and at side surfaces.

By adopting the technical scheme, the adsorption of the target cells in the cell pool 11 by using an antigen-antibody reaction is realized by exchanging the bare structures of the adsorption device 21 in the cell pool 11 and the elution pool 12, and the target cells are eluted in the elution pool 12 at the other side so as to improve the efficiency of separating the cells and realize the cyclic reciprocation, and if the adsorption is repeatedly carried out, compared with the single adsorption, the recovery rate of the target cells is improved; the load groove 211 is a target cell attachment space to protect target cells from being rubbed off by the cell wall 13 during rotation.

In a preferred embodiment of the present invention, the adsorption means 21 is detachably coupled to the rotation shaft 22.

In the specific implementation process, the adsorption device 21 may be provided with holes and grooves to match with the rotation shaft 22, and may be connected by means of sleeving, screwing, or the like.

By adopting the technical scheme, the adsorption device 21 can be detached from the shell 1, so that the adsorption device 21 can load antibodies conveniently, and the adsorption device 21 can be replaced conveniently to adsorb different types of cells.

Referring to fig. 3 and 5, in a preferred embodiment of the present invention, the adsorption device 21 is provided with a transmission hole 212, one end of the rotation shaft 22 passes through the transmission hole 212 and is engaged with the transmission hole 212, and the other end of the rotation shaft 22 passes through the pool wall 13 and is provided with a rotation member 23.

In one embodiment, the rotating member 23 may be a knob or a lever-shaped handle.

By adopting the technical scheme, the rotating piece 23 rotates the rotating shaft 22, so that the adsorbing device 21 is driven to rotate, the adsorbing device 21 is rotated without directly contacting with the rotating piece, and pollution and cell shedding are prevented.

In a preferred embodiment of the present invention, the rotation shaft 22 is provided with a limit groove 221 or a limit projection.

In the specific implementation process, the rotating shaft 22 is matched with the transmission hole 212, the rotating shaft 22 is provided with a limiting groove 221 or a limiting boss, the transmission hole 212 is correspondingly provided with the limiting boss or the limiting groove 221, and the rotating shaft 22 and the transmission hole 212 can be provided with the limiting groove 221 and are connected through keys.

By adopting the technical scheme, the limiting groove 221 or the limiting boss is convenient for transmitting rotary motion.

In a preferred embodiment of the present invention, a plurality of the notches and the adsorption means 21 may be provided in the cell wall 13.

In the specific implementation process, one end of the rotating shaft 22 is connected with the rotating member 23, the other end penetrates through the tank wall 13 and respectively penetrates through the transmission holes 212 of the plurality of adsorption devices 21, when a partition plate is arranged in the elution tank 12 and is divided into independent spaces, and each space is provided with one adsorption device 21, the antibodies loaded on the adsorption devices 21 can be different, and can be provided with a plurality of target cells which are cultured and respectively eluted.

In a preferred embodiment of the present invention, one or two partition bosses 213 are provided on the adsorption device 21.

By adopting the technical scheme, the separation boss 213 is convenient for distinguishing the exchange parts at two sides, and also has the function of limiting, so that excessive rotation is prevented.

In a preferred embodiment of the present invention, two partition projections 213 are provided on the adsorption means 21, and when one of the partition projections 213 is rotated to the top, the other partition projection 213 is brought into contact with the cell wall 13.

By adopting the technical scheme, the end boss 213 at the top end plays a role in prompting, and the separation boss 213 at the bottom end plays a role in limiting. In a specific implementation process, the adsorption device 21 may not be provided with the separation boss 213, but may be divided into two parts by color, lines, marks, scales, and the like.

Referring to fig. 2 and 4, in a preferred embodiment of the present invention, a liquid leakage pool 14 is disposed at the bottom of the casing 1, a liquid leakage channel 131 is disposed on the pool wall 13, and the liquid leakage channel 131 communicates the gap with the liquid leakage pool 14.

By adopting the technical scheme, a small amount of liquid can remain in the load groove 211 in the rotation process of the adsorption device 21, and the liquid leakage channel 131 guides the liquid into the liquid leakage pool 14 to prevent the liquid in the cell pool 11 and the elution pool 12 from mixing.

In a preferred embodiment of the invention, the drip basin 14 is detachably connected to the bottom of the housing 1.

In a specific implementation process, the liquid leakage pool 14 can be connected with the bottom of the shell 1 in a sliding mode through a sliding groove or in a threaded mode.

By adopting the technical scheme, the liquid in the liquid leakage pool 14 can be poured conveniently and the liquid leakage pool 14 can be cleaned conveniently.

In a preferred embodiment of the present invention, the adsorption device 21 is provided with a smooth portion 214, and the load groove 211 is not provided in the smooth portion 214.

In a preferred embodiment of the present invention, the smooth part 214 is provided with an elastic pad, and the elastic pad is made of an elastic material, such as polyphenylene oxide, natural rubber, etc.

By adopting the technical scheme, in the adsorption and elution processes, the adsorption device 21 is in a static state, at the moment, the smooth part 214 is in contact with the notch, the tightness of the contact part is increased, and the tightness can be increased by utilizing the deformation of the elastic pad.

In a preferred embodiment of the present invention, the notch is formed in a semi-surrounding shape, and the rotation shaft 22 is horizontally disposed.

In a specific implementation, when the separation boss 213 is located at the top of the notch, the smooth portion 214 is located at a contact portion with the notch.

By adopting the technical scheme, the separation boss 213 can be observed conveniently through the upper part of the notch, and two exchange parts can be distinguished conveniently, so that the exchange is complete.

In a preferred embodiment of the present invention, a cover 15 is further disposed on the housing 1, and the cover 15 is hinged or detachably connected to the housing 1.

By adopting the technical scheme, the cover body 15 can rotate or separate relative to the shell body 1, so that the opening is realized, and the checking and the operation are convenient; the cover 15 prevents the liquid in the cell wells 11 and the elution well 12 from being contaminated or spilled during the transfer process.

In a preferred embodiment of the present invention, the cover 15 is slidably connected to the housing 1, and the housing 1 is provided with a rib.

By adopting the technical scheme, the convex ribs are convenient for the cover body 15 to apply force to slide, and the cover body 15 is convenient to open and close.

In a preferred embodiment of the present invention, when the cover 15 is in the closed state, the top surface of the partition protrusion 213 is engaged with the bottom surface of the cover 15 to limit the rotation of the suction device 21.

In a specific implementation process, both the bottom surface of the cover 15 and the top surface of the separation boss 213 may be flat and may contact to limit rotation, and the bottom surface of the cover 15 may be provided with a groove matching with the top of the separation boss 213 to limit rotation.

By adopting the technical scheme, when the cover body 15 is closed, the adsorption device 21 is fixed, and the two exchange parts are prevented from rotating in the transfer process to influence the adsorption and elution effects.

It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A cell separation device is characterized by comprising a shell (1) and an adsorption component (2), a cell pool (11) and an elution pool (12) are arranged in the shell (1), a cell wall (13) is formed between the cell pool (11) and the elution pool (12), the tank wall (13) is provided with a gap, a rotating shaft (22) is arranged in the tank wall (13) at the gap, the adsorption component (2) comprises an adsorption device (21), the adsorption device (21) is positioned at the gap, the adsorption device (21) is connected with a rotating shaft (22) on the cell pool wall (13) in match with the gap, and can realize the exchange between the cell pool (11) and the partial structure of the elution pool (12), the adsorption device (21) is provided with a load groove (211), and the load groove (211) is used for loading the antibody.

2. The cell separation apparatus according to claim 1, wherein: the adsorption device (21) is detachably connected with the rotating shaft (22).

3. The cell separation apparatus according to claim 2, wherein: the adsorption device (21) is provided with a transmission hole (212), one end of the rotating shaft (22) penetrates through the transmission hole (212) and is matched with the transmission hole (212), and the other end of the rotating shaft (22) penetrates through the pool wall (13) and is provided with a rotating piece (23).

4. The cell separation apparatus according to any one of claims 1 to 3, wherein: a plurality of notches and adsorption devices (21) on the pool wall (13) can be arranged.

5. The cell separation apparatus according to claim 4, wherein: one or two separating bosses (213) are arranged on the adsorption device (21).

6. The cell separation apparatus according to claim 5, wherein: the bottom of the shell (1) is provided with a liquid leakage pool (14), the pool wall (13) is provided with a liquid leakage channel (131), and the liquid leakage channel (131) is communicated with the notch and the liquid leakage pool (14).

7. The cell separation apparatus according to claim 6, wherein: the notch is in a semi-surrounding shape, and the rotating shaft (22) is horizontally arranged.

8. The cell separation apparatus according to claim 7, wherein: the shell (1) is further provided with a cover body (15), and the cover body (15) is hinged or detachably connected with the shell (1).

9. The cell separation apparatus according to claim 8, wherein: when the cover body (15) is in a closed state, the top surface of the separation boss (213) is matched with the bottom surface of the cover body (15) to limit the rotation of the adsorption device (21).