CN112251384A - Sinorhizobium strain with phosphate solubilizing capability and application thereof - Google Patents
Sinorhizobium strain with phosphate solubilizing capability and application thereof Download PDFInfo
- Publication number
- CN112251384A CN112251384A CN202011189260.XA CN202011189260A CN112251384A CN 112251384 A CN112251384 A CN 112251384A CN 202011189260 A CN202011189260 A CN 202011189260A CN 112251384 A CN112251384 A CN 112251384A
- Authority
- CN
- China
- Prior art keywords
- sinorhizobium
- strain
- ytr
- phosphorus
- corn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/41—Rhizobium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a Sinorhizobium (sp) strain with phosphate solubilizing capability, which is characterized in that: the strain is Sinorhizobium (Sinorhizobium. sp) strain YTR-S1, which has been preserved in China general microbiological culture collection center at 9/18 th 2020 with the preservation number of CGMCC 20681. Based on a long-term positioning test of organic fertilization of a hawkthorn pond red soil ecological experimental station of Chinese academy of sciences, the invention screens out a high-efficiency phosphate solubilizing bacterium from corn rhizosphere soil, performs sequencing identification on the phosphate solubilizing bacterium, proves that a strain YTR-S1 has the capability of efficiently dissolving inorganic phosphorus and organic phosphorus, and discovers that the content of effective phosphorus in red soil is remarkably increased and the growth condition and quality of corn are improved by adding the strain YTR-S1 through pot experiments.
Description
Technical Field
The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a Sinorhizobium strain and application thereof in phosphate solubilizing.
Background
Phosphorus (P) is one of important element indexes for measuring soil fertility, and is also an essential element for metabolic processes such as synthesis of protein and phosphoric acid in plants, energy circulation and the like. The dry land red soil in the southeast hilly area is an important base for tropical and subtropical economic forest fruits, economic crops and grain production in China, and plays an important role in agricultural sustainable development and ecological environment construction in China. The total phosphorus content in the red soil is higher, wherein inorganic phosphorus accounts for 35-70% of the total phosphorus, organic phosphorus accounts for 30-65% of the total phosphorus, but a large amount of phosphorus is covered by metal ions Al3+、Fe3+And Ca2+When the phosphorus is adsorbed and fixed, the phosphorus exists in an invalid state, so that the phosphorus deficiency phenomenon of the southern red soil is common. At present, the most extensive method for solving the lack of phosphorus in soil is to apply chemical phosphate fertilizer, but the use of a large amount of phosphate fertilizer not only increases the agricultural production cost, but also easily causes deterioration of physical and chemical properties of soil, acidification and hardening of texture, reduction of productivity of an ecological system, increase of accumulation of toxic elements and heavy metals in soil and the like. Therefore, the selection of a green sustainable fertilization mode has important significance for developing green and healthy agriculture and ecological environment protection.
A large amount of phosphate-solubilizing microbial resources are contained in the soil. The functional microorganisms can activate inorganic phosphate and organic phosphorus which are insoluble in soil and cannot be utilized by plants, obviously improve the nutrient level of soluble phosphorus in the soil, increase the absorption and utilization of the plants on phosphorus elements, and further promote the growth of the plants. Therefore, in order to relieve the stress of the phosphorus in the red soil and realize high and stable yield of crops, the high-efficiency indigenous phosphate solubilizing bacteria suitable for the red soil in the dry land can be screened and purified from the soil, the culture conditions of the high-efficiency indigenous phosphate solubilizing bacteria are optimized, and the prepared microbial inoculum is applied to the acid red soil in the dry land with low effective phosphorus content to exert the high-efficiency phosphate solubilizing effect of the microbial inoculum and improve the utilization efficiency of the phosphate fertilizer in the red soil, and partially replaces the inorganic phosphate fertilizer and promotes the stable yield and the yield of the crops. At present, the phosphorus-solubilizing bacteria suitable for the acid red soil and promoting the yield increase of crops are only reported, and how to utilize the phosphorus-solubilizing bacteria to prepare a microbial inoculum to improve the activation and release of insoluble phosphorus in the soil has important application value.
Disclosure of Invention
The invention aims to provide a sinorhizobium strain with phosphorus solubilizing capability, so as to activate red soil phosphorus, improve the effectiveness of the soil phosphorus, reduce the using amount of chemical fertilizers and promote the growth and yield of corns.
In order to achieve the purpose, the invention adopts the technical scheme that:
a Sinorhizobium (Sinorhizobium. sp) strain YTR-S1 with phosphorus-solubilizing ability is preserved in China general microbiological culture collection center at 9/18 th of 2020 with the preservation number of CGMCC 20681.
The sinorhizobium strain YTR-S1 is separated from corn rhizosphere soil in a cell for organic fertilization long-term positioning test at a eagle pond red soil ecological experimental station of Chinese academy of sciences, and is diluted by soil suspension, inoculated by a KB culture medium, cultured, separated and purified to obtain a sinorhizobium (Sinorhizobium.sp) strain which is named YTR-S1 and has the gene registration number MW039077 at Genbank.
The biological characteristics of the strain are as follows:
(1) morphological characteristics: milky white round colonies were formed on KB solid medium with regular edges, convex shape, smooth and moist surface, and gram-negative stain, see FIG. 1.
(2) The culture characteristics are as follows: the optimum growth temperature is in the range of 25-30 deg.C, and the preferable growth pH is in the range of 6.0-8.0 (see FIG. 2), and aerobically grown in KB medium. The components of the culture medium: peptone 20.0g/L, C3H8O3 10mL/L，K2HPO41.5g/L，MgSO4·7H2O 1.5g/L。
It is a second object of the present invention to provide a microbial agent comprising Sinorhizobium sp strain YTR-S1.
A third object of the present invention is to provide the use of the aforementioned sinorhizobium (sinorhizobium sp) strain YTR-S1.
The Sinorhizobium (Sinorhizobium. Sp) strain YTR-S1 has high phosphorus dissolving effect, and can decompose inorganic phosphorus and organic phosphorus, such as aluminum phosphate, iron phosphate, and lecithin.
The Sinorhizobium (Sinorhizobium.sp) strain YTR-S1 is used for improving the growth condition of corn, and is prepared into a microbial inoculum which is applied to red soil to improve the growth condition of corn, so that the biomass of the overground part and the underground part of the corn can be increased, the content of soluble sugar, soluble protein and indoleacetic acid (IAA) in leaves can be increased, and the content of available phosphorus in the red soil can be increased.
Has the advantages that: the invention screens out a high-efficiency phosphate solubilizing bacterium from the corn rhizosphere soil of a long-term positioning test cell for organic fertilization of a hawkthorn pond red soil ecological experimental station of Chinese academy of sciences, and the sequencing identification proves that YTR-S1 has the high-efficiency phosphate solubilizing characteristic, and the phosphate solubilizing bacterium is prepared for use as a microbial inoculum, so that the content of available phosphorus in red soil is obviously increased, and the growth condition of corn is improved.
Drawings
FIG. 1 is a characteristic diagram of the colony of YTR-S1 strain isolated in the present invention on KB solid medium;
FIG. 2 is a graph of the growth curve of YTR-S1 strain at different pH;
FIG. 3 is a picture of the phosphate solubilizing circle of YTR-S1 strain;
FIG. 4 is a diagram of construction of phylogenetic tree of YTR-S1 strain;
FIG. 5 is a graph showing the growth vigor of corn on day 15 and day 30 after the inoculation of YTR-S1 strain in a potting experiment;
FIG. 6 shows the height of maize, soluble sugar in leaves, soluble protein in leaves, indoleacetic acid (IAA) in leaves, biomass of underground parts of the overground part, and the content of available phosphorus in soil, which are inoculated with YTR-S1 strain in a potting experiment, respectively.
Detailed Description
The present invention will be further explained with reference to examples.
The present invention will be better understood from the following examples. However, one skilled in the art will readily appreciate that the specific material proportions, process conditions, and results thereof described in the examples are merely illustrative of the invention and should not, nor should they, limit the invention as detailed in the claims.
Example 1
Screening and characterization of Sinorhizobium (Sinorhizobium. sp.) Strain YTR-S1
1. Screening process
(1) Weighing 1g of corn rhizosphere soil, putting the corn rhizosphere soil into a centrifuge tube, adding 9mL of 0.9% sterilized normal saline to prepare 10%-1The bacterial suspension is put into a shaking box of 180r/min for shaking for 10min, and then is taken out and stands for 1 h.
(2) Sucking 5 μ L of sterile water and 45 μ L of sterile water from the soil suspension after standing to obtain a solution of 10-2And (4) bacterial suspension. Sequentially diluting the bacterial suspension to 10-3、10-4、10-5Respectively taking 5ml 10-5The bacterial suspension was added to 50mL and 500mL KB medium to prepare 1-0-6And 10-7The culture solution of (4) was 250. mu.L of 10-6And 10-7The culture solution of (2) was added to a 96-well plate. Finally, the 96-well plate is placed into a microplate reader, and the growth condition of the 96-well plate is observed under room temperature culture.
(3) Taking 200 mul of liquid from the aperture of the bacteria liquid growing in the plate to a storage tube, adding 200 mul of glycerol for storage, correspondingly marking the serial numbers of the pore plate and the pore, and using the remaining 50 mul for colony PCR amplification. PCR amplification conditions: pre-denaturation at 94 ℃ for 10min, denaturation at 94 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 1min, and denaturation and annealing extension for 35 cycles, and terminating the reaction after extension at 72 ℃ for 10 min. The valid sequences obtained by sequencing were compared at NCBI and showed the highest homology to Sinorhizobium.
The gene sequence of the Sinorhizobium sp strain YTR-S1 is shown in SEQ ID NO. 1.
Example 2
Phosphate solubilizing Properties of Sinorhizobium (Sinorhizobium. sp.) Strain YTR-S1
(1) The phosphate ring dissolving method is used. The organic phosphorus source is lecithin, and the inorganic phosphorus source is aluminum phosphate and iron phosphate, which are all commercially available analytical pure reagents.
(2) Preparing Monkinna organic phosphorus (lecithin) and phosphoric acidThe method comprises the following steps of dividing each plate into 6 areas, sequentially sucking 2 mu L of points at the center of each area, culturing for 3D in an incubator at 28 ℃, observing whether a strain grows and forms a phosphate solubilizing ring, recording the diameter (unit D/cm) of the phosphate solubilizing ring and the diameter (unit D/cm) of a bacterial colony, and calculating the D/D ratio so as to determine the phosphate solubilizing capability and the phosphate solubilizing efficiency of the strain on different insoluble phosphates. The formula of the culture medium is as follows: culture medium (1L) of Monkinna organophosphorus (lecithin) bacteria: glucose 10.0g, (NH)4)2SO4 0.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO40.03g，MnSO4·H2O 0.03g，KCl 0.3g，CaCO31.0g, lecithin 0.3g, agar 20g, pH 7.0. Wherein lecithin is dissolved in 75% ethanol under heating, sterilized separately, mixed with sterilized culture medium cooled to 60 deg.C, and poured onto a flat plate. ② Monkinna inorganic phosphorus (aluminum phosphate) bacteria culture medium (1L): glucose 10.0g, (NH)4)2SO4 0.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO4 0.03g，MnSO4·H2O 0.03g，KCl 0.3g，CaCO3 1.0g，AlPO43.93g, agar 20g, pH 7.0. ③ Monkinna inorganic phosphorus (iron phosphate) bacteria culture medium (1L): glucose 10.0g, (NH)4)2SO4 0.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO4 0.03g，MnSO4·H2O 0.03g，KCl 0.3g，CaCO3 1.0g，FePO44.86g, agar 20g, pH 7.0. The results show that the sinorhizobium YTR-S1 can grow on the three culture medium plates and has certain dissolving capacity on lecithin, ferric phosphate and aluminum phosphate. The results of the phosphate solubilizing cycle are shown in FIG. 3. The dissolving capacity of the strain to the insoluble phosphorus is shown in the table 1, and the ratio of the diameter of the phosphorus dissolving ring to the diameter of the bacterial colony is aluminum phosphate>Lecithin>Iron phosphate, 2.67, 1.90, 1.74 respectively.
TABLE 1 Sinorhizobium YTR-S1 phosphorus solubilization characterization
Example 3
Experiment of potted corn
1. The experiment was carried out in two treatments, Sinorhizobium YTR-S1 and control treatment (CK) without inoculum, each of which was repeated 5 times.
2. The using amount of soil and fertilizer: the organic fertilizer and the red soil are fully mixed according to the content (mass content) of the organic fertilizer in the soil being 2 percent, the mixture is sterilized and is loaded into plastic pots with the upper caliber of 10cm, the lower caliber of 7cm and the height of 8.5cm, and each pot of the plastic pots contains 500g of soil matrix.
3. Seedling culture: the corn seed is Suyu No. 24. 1) Seed disinfection: first, the solution is sterilized by 70% alcohol for 1min, then sterilized by 1% sodium hypochlorite for 15min, and finally washed by sterile water for five times. Placing the sterilized seeds on sterile water, and placing the sterilized seeds in an incubator at 28 ℃ for dark culture until the seeds germinate; 2) and selecting the seedlings with consistent growth vigor on the fifth day of seed germination and transplanting the seedlings into potted red soil.
4. Adding a bacterial liquid: and adding bacterial liquid once on each of the 3 rd day and the 15 th day after the corn seedlings are transplanted, and adding 5mL of bacterial liquid into each pot according to the amount of 100 mu L of soil per gram. Respectively culturing 100mL YTR-S1 bacterial liquid in KB culture medium by shaking, and respectively measuring OD of three fermentation liquors after culturing for 24h600Value, when OD600When the value reaches 1.0, the concentration of the bacterial liquid is about 1 multiplied by 109CFU·mL-1The bacterial liquid is centrifuged, washed and precipitated for 3 times by 0.9 percent physiological saline, and finally the thalli is suspended by sterile water to prepare a bacterial agent which is added into soil.
5. After a pot experiment is carried out for one month, corn plants are harvested, and the biomass of the overground part and the underground part of each processed corn seedling, soluble sugar in leaves, soluble protein in the leaves, indoleacetic acid (IAA) in the leaves and the content of available phosphorus in red soil are respectively measured. The results are shown in FIG. 6: the plant height of the plant added with the fungicide YTR-S1 is improved by 13.7 percent compared with that of a control CK, the plant biomass is 2.0 times of that of the CK, wherein the biomass of the overground part is 71.7 percent higher than that of the CK, and the biomass of the underground part is 212.93 percent higher than that of the CK; the content of soluble sugar, soluble protein, indoleacetic acid (IAA) and red soil available phosphorus in the plant leaves added with the fungicide YTR-S1 are respectively 124.3%, 113.5%, 31.7% and 22.6% higher than CK.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Nanjing soil institute of Chinese academy of sciences
<120> Sinorhizobium strain with phosphate solubilizing capability and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1312
<212> DNA
<213> genus Sinorhizobium (sp)
<400> 1
gtagaaccaa ctcccatggt gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc 60
gcagcatgct gatctgcgat tactagcgat tccaacttca tgcactcgag ttgcagagtg 120
caatccgaac tgagatggct tttggagatt agctcgacct cgcggtctcg ctgcccactg 180
tcaccaccat tgtagcacgt gtgtagccca gcccgtaagg gccatgagga cttgacgtca 240
tccccacctt cctctcggct tatcaccggc agtcccctta gagtgcccaa ccaaatgctg 300
gcaactaagg gcgagggttg cgctcgttgc gggacttaac ccaacatctc acgacacgag 360
ctgacgacag ccatgcagca cctgtctccg atccagccga actgaaggaa aacgtctccg 420
taatccgcga tcgggatgtc aagggctggt aaggttctgc gcgttgcttc gaattaaacc 480
acatgctcca ccgcttgtgc gggcccccgt caattccttt gagttttaat cttgcgaccg 540
tactccccag gcggaatgtt taatgcgtta gctgcgccac cgaacagtaa actgcccgac 600
ggctaacatt catcgtttac ggcgtggact accagggtat ctaatcctgt ttgctcccca 660
cgctttcgca cctcagcgtc agtaatggac cagtgagccg ccttcgccac tggtgttcct 720
ccgaatatct acgaatttca cctctacact cggaattcca ctcacctctt ccatactcta 780
gacacccagt atcaaaggca gttccggggt tgagccccgg gatttcaccc ctgacttaaa 840
tgtccgccta cgtgcgcttt acgcccagta attccgaaca acgctagccc ccttcgtatt 900
accgcggctg ctggcacgaa gttagccggg gcttcttctc cggttaccgt cattatcttc 960
accggtgaaa gagctttaca accctagggc cttcatcact cacgcggcat ggctggatca 1020
ggcttgcgcc cattgtccaa tattccccac tgctgcctcc cgtaggagtt tgggccgtgt 1080
ctcagtccca atgtggctga tcatcctctc agaccagcta tggatcgtcg ccttggtagg 1140
cctttacccc accaactagc taatccaacg cgggctcatc ctttcccgat aaatctttcc 1200
cccaaagggc ttatacggta ttagcacaag tttccctgcg ttattccgta gaaaagggta 1260
gattcccacg cgttactcac ccgtctgccg ctccccttgc ggggcgctcg ac 1312
Claims (7)
1. A Sinorhizobium (sp) strain having phosphate solubilizing ability, characterized by: the strain is Sinorhizobium (Sinorhizobium. sp) strain YTR-S1, which has been preserved in China general microbiological culture collection center at 9/18 th 2020 with the preservation number of CGMCC 20681.
2. A microbial agent comprising the strain of sinorhizobium (sinorhizobium.sp) of claim 1.
3. Use of a strain of the genus sinorhizobium (sinorhizobium.sp) according to claim 1 for phosphate solubilization.
4. Use according to claim 3, characterized in that: the Sinorhizobium (Sinorhizobium. sp) strain YTR-S1 is capable of decomposing inorganic phosphorus and organic phosphorus.
5. Use according to claim 4, characterized in that: the Sinorhizobium (Sinorhizobium. sp) strain YTR-S1 can decompose aluminum phosphate, iron phosphate and lecithin.
6. Use according to claim 3, characterized in that: the Sinorhizobium (Sinorhizobium. sp) strain YTR-S1 is used for improving the growth status of corn.
7. Use according to claim 3, characterized in that: the Sinorhizobium (Sinorhizobium. sp) strain YTR-S1 is prepared into a microbial inoculum which is applied to red soil to improve the growth condition of corn, increase the biomass of the overground part and the underground part of the corn, increase the content of soluble sugar, soluble protein and indoleacetic acid (IAA) in the corn leaves and increase the content of available phosphorus in the red soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011189260.XA CN112251384B (en) | 2020-10-30 | 2020-10-30 | Sinorhizobium strain with phosphate solubilizing capability and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011189260.XA CN112251384B (en) | 2020-10-30 | 2020-10-30 | Sinorhizobium strain with phosphate solubilizing capability and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112251384A true CN112251384A (en) | 2021-01-22 |
| CN112251384B CN112251384B (en) | 2021-04-13 |
Family
ID=74267852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011189260.XA Active CN112251384B (en) | 2020-10-30 | 2020-10-30 | Sinorhizobium strain with phosphate solubilizing capability and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112251384B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115404183A (en) * | 2022-08-24 | 2022-11-29 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application |
| CN116024124A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of Sword bacteria S2_8_1 in promoting plant to synthesize cytokinin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838655A (en) * | 2016-06-14 | 2016-08-10 | 农业部环境保护科研监测所 | Low-temperature phosphate solubilization Sinorhizobium fredii and inoculant thereof |
| CN106987541A (en) * | 2017-05-03 | 2017-07-28 | 新疆农业大学 | One plant has degeneration-resistant, growth-promoting performance efficient rhizobium melioti and its application |
| CN110713953A (en) * | 2019-11-15 | 2020-01-21 | 中国科学院南京土壤研究所 | Mesorhizobium strain with phosphate solubilizing property and application thereof |
-
2020
- 2020-10-30 CN CN202011189260.XA patent/CN112251384B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838655A (en) * | 2016-06-14 | 2016-08-10 | 农业部环境保护科研监测所 | Low-temperature phosphate solubilization Sinorhizobium fredii and inoculant thereof |
| CN106987541A (en) * | 2017-05-03 | 2017-07-28 | 新疆农业大学 | One plant has degeneration-resistant, growth-promoting performance efficient rhizobium melioti and its application |
| CN110713953A (en) * | 2019-11-15 | 2020-01-21 | 中国科学院南京土壤研究所 | Mesorhizobium strain with phosphate solubilizing property and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| LI,JIANFENG等: "Effect of Phosphate Solubilizing Rhizobium Strains on Alfalfa Seedlings Growth Under Nitrogen Free and Phosphorus Deficient Conditions", 《JOURNAL OF BIOBASED MATERIALS AND BIOENERGY》 * |
| 曾晓希等: "中华根瘤菌NL的筛选与鉴定", 《湖北农业科学》 * |
| 杨妍等: "西双版纳保护区植物根际细菌的筛选及其促生能力研究", 《微生物学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115404183A (en) * | 2022-08-24 | 2022-11-29 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application |
| CN115404183B (en) * | 2022-08-24 | 2024-01-26 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application thereof |
| CN116024124A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of Sword bacteria S2_8_1 in promoting plant to synthesize cytokinin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112251384B (en) | 2021-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109852565B (en) | Saline-alkali soil composite modifier and application method thereof | |
| CN109810924B (en) | Method for improving severe saline-alkali soil | |
| CN110616171B (en) | Saline-alkali-resistant Pacific bacillus and viable bacteria preparation and application thereof | |
| CN107136122B (en) | Biocontrol microbial inoculum for preventing and treating potato late blight | |
| CN111690578B (en) | Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof | |
| CN112111427B (en) | Bacillus subtilis PA8 and application thereof | |
| CN108192838B (en) | Bacillus amyloliquefaciens with dual functions of inorganic phosphorus degradation and disease prevention | |
| CN112251384B (en) | Sinorhizobium strain with phosphate solubilizing capability and application thereof | |
| CN113621532A (en) | Compound microbial agent containing saline-alkali-resistant bacillus paraclicheniformis and preparation method thereof | |
| CN113875548A (en) | Application of bacillus beilesiensis BV03 in preparation of seedling raising substrate for promoting vegetable growth | |
| CN110892805B (en) | Application of biological stimulin for improving salt tolerance of corn seed germination | |
| CN108913625B (en) | Salt-tolerant streptomycete, microbial inoculum thereof and application of microbial inoculum thereof in promoting plant growth | |
| CN116904361B (en) | Copper bacteria strain with phosphate dissolving capability and application thereof | |
| CN108795797B (en) | Corn root system endophytic enterobacter cloacae and application thereof | |
| CN108048360B (en) | Bacillus subtilis with dual functions of degrading organic phosphorus and preventing diseases | |
| CN110713953B (en) | Mesorhizobium strain with phosphate solubilizing property and application thereof | |
| RU2415924C1 (en) | Sinorhizobium fredii RNCM NO B-2458D NODULE BACTERIAL STRAIN FOR MANUFACTURING OF BACTERIAL SOYA FERTILISER | |
| CN102763685B (en) | A kind of banana vascular wilt resistant fermentation liquor and its preparation method and application | |
| CN114854637A (en) | Phosphorus-dissolving growth-promoting bacillus tropicalis SG15, biological agent and application of biological agent in potato seed dressing agent | |
| CN109957535B (en) | Bacillus simplex, microbial agent prepared by using bacillus simplex, biological fertilizer and application | |
| CN111909863B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN113897316A (en) | Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application of functional melon and fruit seedling biological matrix | |
| CN109337844B (en) | Pseudomonas moraxella X2 and application thereof | |
| CN111778197A (en) | Methylobacterium strain with high growth promoting efficiency and application thereof | |
| CN111607535A (en) | Application of cycloserine and beneficial bacteria in cooperation for preventing and controlling soil-borne bacterial wilt of tomatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |