CN112251355A

CN112251355A - Cell culture device and culture method thereof

Info

Publication number: CN112251355A
Application number: CN202011224603.1A
Authority: CN
Inventors: 杨宇艳
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2021-01-22

Abstract

The invention discloses a cell culture device and a culture method thereof, wherein the cell culture device comprises a culture device, the culture device is used for culturing cells, a partition plate is arranged in the middle of the culture device, a cavity in the culture device is divided into a first containing cavity and a second containing cavity by the partition plate, the first containing cavity and the second containing cavity are both used for culturing the cells, the partition plate is used for separating the first containing cavity from the second containing cavity, two scale marks are arranged on the front surface of the culture device, and the two scale marks are respectively used for measuring solutions in the first containing cavity and the second containing cavity. The invention has the beneficial effects that: according to the cell culture device and the cell culture method, the partition plate is arranged in the culture device, the control group can be arranged during cell culture, cell culture can be better performed, meanwhile, the method for culturing cells can be improved through the arrangement of the illumination, and the cells can be better grown by continuously adding the culture medium during culture.

Description

Cell culture device and culture method thereof

Technical Field

The invention relates to a culture device, in particular to a cell culture device and a culture method thereof, belonging to the technical field of culture devices.

Background

Most cells are tiny, beyond the limits of human vision. The cells must be observed under a microscope. However, until the objective presence of cells is recognized, it has not been known that the object observed under a microscope is a cell. Therefore, when the animal sperm was observed in the lewenke by a simple microscope made by itself in 1677 years, it was not known that it was a cell. The term cell is named after 1667 r. hooke, who sees the cork containing individual chambers when looking at the section of the cork. In fact, these chambers are not living structures, but rather are voids formed by cell walls, but the term cell is used as follows. In the initial stage of cytology, the objective is primarily to observe developmental phenomena such as butterfly metamorphosis, sperm and egg structures, etc., although many tiny objects such as bacteria, ciliates, etc., are also observed with a simple microscope. Due to the limitation of the microscope at that time, the observation is not accurate enough, and the constraint of religious beliefs is added, the observation results support the prejudicial textbook instead. Someone claims to see a specific and tiny human among the sperm, who is thought to develop this into a future individual-a sperm-only; others also consider minuscule to be present in eggs-roemer only. The effect of the first-come theory has lasted for over 100 years, preventing people from further understanding the cells on the basis of r. hooke, and until m. The achromatic objective lens developed before and after this, introduced carmine (carmine) and hematoxylin as dyes for staining cell nuclei and the initial creation of microtome and sectioning techniques, all created advantages for finer cell observation.

What has played a great driving role for studying cells are m.j. schleiden and t.a.h. schwann. The former described in 1838 that cells were produced in a mucus-like matrix through a process like crystallization and first produced nuclei (nucleoli was also found). He and regarded the plant as a community of cells, as is the case with the hydroid population. Under his initiative, Shiwang believes that both animals and plants are composed of cells. He accumulated a number of facts indicating the consistency of both in structure and growth, and presented a cytological dogma in 1839. Meanwhile, czech zoophysiologist j.e. purkinje proposed the concept of protoplasm; the german zoologist c.t.e.von siboder (1845) concluded that the protozoa were all single-celled. The german pathologist r.c. fel shaw (1855) proposed a name for all cells from cells on the basis of the study of connective tissue and established cytopathology. German zoologist m. sulzer defined the cells in 1861: the cell is a mass of plasma with all vital features, in which the nucleus is located.

The prior cell culture device can not well culture cells, and the culture method has defects, so the cell culture device and the culture method thereof are provided.

Disclosure of Invention

The present invention is directed to a cell culture apparatus and a culture method thereof, which solve the above problems of the background art.

The invention achieves the aim through the following technical scheme, and the cell culture device and the cell culture method thereof comprise a culture device, wherein the culture device is used for culturing cells, a partition plate is arranged in the middle of the culture device, a cavity in the culture device is divided into a first containing cavity and a second containing cavity by the partition plate, the first containing cavity and the second containing cavity are both used for culturing the cells, and the partition plate is used for separating the first containing cavity from the second containing cavity.

Preferably, the front surface of the incubator is provided with two scale marks, and the two scale marks are respectively used for measuring the solution in the first cavity and the solution in the second cavity.

Preferably, the incubator is made of glass.

Preferably, a cell culture method comprises the following steps:

s1: placing cells to be cultured on a glass slide, then sterilizing the incubator, and cleaning the incubator by using a cell culture solution after sterilizing at high temperature;

s2: after the incubator is cleaned, cells to be cultured are put into the incubator together with the glass slide, and the cells put into the first cavity and the cells put into the second cavity are the same;

s3: and adding culture medium into the first cavity and the second cavity, and setting the cells in the first cavity as a control group.

Preferably, the cells are cultured by placing the incubator in a sterile environment, and the cells are added with reference to the scale marks when the medium is added.

Preferably, the cells in the incubator are cultured at a temperature of 22-28 ℃.

Preferably, the medium is added to the medium every other day in an amount of 35-50 ml per addition.

Preferably, during the culture, the incubator is covered by a cover, and the cover is provided with two small holes which are respectively communicated with the first cavity and the second cavity.

The invention has the beneficial effects that: the culture device is provided with the partition board, when cell culture is carried out, the setting of the control group can be carried out, the cell culture can be better carried out, meanwhile, the method for culturing the cells can be improved through the arrangement of the illumination, and when the cell culture is carried out, the cells can be better grown through continuously adding the culture medium.

Drawings

Fig. 1 is a schematic front structural view of the present invention.

In the figure: 1. a culture device; 2. a partition plate; 3. a first cavity; 4. a second cavity; 5. scale lines are marked.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Please refer to fig. 1:

the first embodiment is as follows:

a cell culture device and a culture method thereof comprise a culture device 1, wherein the culture device 1 is used for culturing cells, a partition plate 2 is arranged in the middle of the culture device 1, a cavity in the culture device 1 is divided into a first containing cavity 3 and a second containing cavity 4 by the partition plate 2, the first containing cavity 3 and the second containing cavity 4 are both used for culturing the cells, and the partition plate 2 is used for separating the first containing cavity 3 from the second containing cavity 4.

The front of incubator 1 is provided with two scale marks 5, and two scale marks 5 are used for holding the interior solution of chamber 3 and second chamber 4 and carrying out the measurement respectively, and incubator 1 is the glass material.

A cell culture method comprising the following methods:

the cell that will need to cultivate is placed on the slide glass, then disinfects the incubator, utilizes high temperature disinfection back, utilizes cell culture solution to wash incubator 1, and incubator 1 washs the back, puts into incubator 1 together with the slide glass the cell that will need to cultivate, and first appearance chamber 3 is the same with the cell that second appearance chamber 2 put into, adds the culture medium in first appearance chamber 3 and second appearance chamber 4, sets up the cell in first appearance chamber 3 into the control group simultaneously.

When the cells are cultured, the incubator 1 is placed in a sterile environment for culturing, when the culture medium is added, the reference scale mark 5 is added, when the cells in the incubator 1 are cultured, the temperature of the culture is 22 ℃, the culture medium is added into the culture medium 1 every other day, the adding amount is 35 ml each time, when the cells are cultured, the incubator 1 is covered by a cover, and the cover is provided with two small holes which are respectively communicated with the first containing cavity 3 and the second containing cavity 4.

Example two:

A cell culture method comprising the following methods:

When the cells are cultured, the incubator 1 is placed in a sterile environment for culturing, when the culture medium is added, the reference scale mark 5 is used for adding, when the cells in the incubator 1 are cultured, the temperature of the culture is 25 ℃, the culture medium is added into the culture medium 1 every other day, the adding amount is 42 milliliters each time, when the cells are cultured, the incubator 1 is covered by a cover, and the cover is provided with two small holes which are respectively communicated with the first containing cavity 3 and the second containing cavity 4.

Example three:

A cell culture method comprising the following methods:

When cells are cultured, the incubator 1 is placed in a sterile environment for culturing, when a culture medium is added, the reference scale mark 5 is used for adding, when the cells in the incubator 1 are cultured, the temperature of the culture is 28 ℃, the culture medium is added into the culture medium 1 every other day, the adding amount is 50 milliliters each time, when the cells are cultured, the incubator 1 is covered by a cover, and the cover is provided with two small holes which are respectively communicated with the first containing cavity 3 and the second containing cavity 4.

In the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "mounted," "connected," and "secured" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integral to; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The standard parts used by the invention can be purchased from the market, and the special-shaped parts can be customized according to the description and the description of the attached drawings.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture apparatus comprising an incubator (1), characterized in that: the incubator (1) is used for culturing cells, the middle part of the incubator (1) is provided with the partition plate (2), the cavity inside the incubator (1) is divided into the first accommodating cavity (3) and the second accommodating cavity (4) by the partition plate (2), the first accommodating cavity (3) and the second accommodating cavity (4) are both used for culturing the cells, and the partition plate (2) is used for separating the first accommodating cavity (3) from the second accommodating cavity (4).

2. A cell culture apparatus according to claim 1, wherein: the front surface of the incubator (1) is provided with two scale marks (5), and the two scale marks (5) are respectively used for measuring the solution in the first accommodating cavity (3) and the solution in the second accommodating cavity (4).

3. A cell culture apparatus according to claim 1, wherein: the incubator (1) is made of glass.

4. A method of cell culture, comprising: the method comprises the following steps:

s1: placing cells to be cultured on a glass slide, then sterilizing the incubator, and cleaning the incubator (1) by using a cell culture solution after sterilizing at high temperature;

s2: after the incubator (1) is cleaned, cells to be cultured are put into the incubator (1) together with the glass slide, and the cells put into the first cavity (3) and the second cavity (2) are the same;

s3: and adding culture medium into the first cavity (3) and the second cavity (4), and setting the cells in the first cavity (3) as a control group.

5. A cell culture method according to claim 4, wherein: when cells are cultured, the incubator (1) is placed in a sterile environment for culturing, and when a culture medium is added, the cells are added with reference to the scale marks (5).

6. A cell culture method according to claim 4, wherein: when the cells in the incubator (1) are cultured, the temperature for culturing is 22-28 ℃.

7. A cell culture method according to claim 4, wherein: the medium was added to the medium (1) every other day in an amount of 35-50 ml per addition.

8. A cell culture method according to claim 4, wherein: during culture, the incubator (1) is covered by a cover, and the cover is provided with two small holes which are respectively communicated with the first containing cavity (3) and the second containing cavity (4).