CN112250171A - Device and method for starting short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as carbon source - Google Patents
Device and method for starting short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as carbon source Download PDFInfo
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- 239000010865 sewage Substances 0.000 title claims abstract description 59
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 25
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 150000004056 anthraquinones Chemical class 0.000 title claims abstract description 23
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 39
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000002351 wastewater Substances 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 13
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000009825 accumulation Methods 0.000 claims abstract description 5
- 238000005086 pumping Methods 0.000 claims abstract description 5
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000010802 sludge Substances 0.000 claims description 6
- 239000005416 organic matter Substances 0.000 claims description 5
- 238000004364 calculation method Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 7
- 108090000913 Nitrate Reductases Proteins 0.000 abstract description 3
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 abstract description 3
- 230000001360 synchronised effect Effects 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 229910021529 ammonia Inorganic materials 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010025915 Nitrite Reductases Proteins 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A device and a method for starting short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as a carbon source belong to the field of biological treatment. At present, the simple carbon source is added instead of directly utilizing organic matters in domestic sewage as the carbon source to start the short-range denitrification, so that the operation cost is increased, and the aim of starting the short-range denitrification by utilizing the organic matters in the domestic sewage as the carbon source is fulfilled by adding anthraquinone. Anthraquinone as redox mediator can improve the activity of nitrate reductase and realize the promotion of nitrite accumulation. The device comprises a nitrate wastewater inlet tank, a domestic sewage inlet tank, a short-distance denitrification reactor, a water outlet tank and an automatic control platform. And (3) pumping the nitrate wastewater and the domestic sewage into the short-range denitrification reactor according to the carbon-nitrogen ratio, and then adding anthraquinone to realize the conversion from nitrate nitrogen to nitrite nitrogen by using organic matters in the domestic sewage as a carbon source. The method does not need an additional carbon source, realizes the synchronous removal of the nitrate wastewater and the domestic sewage, and has the advantages of carbon source saving and the like.
Description
Technical Field
The invention relates to a device and a method for starting short-cut denitrification by using organic matters in domestic sewage as a carbon source by adding anthraquinone, belongs to the technical field of biological sewage treatment, and relates to an experimental device and a method for synchronously removing nitrate wastewater and domestic sewage.
Background
The nitrogen pollution in the water body is more and more serious, and the frequent occurrence of eutrophication causes the high attention of people. How to efficiently and economically remove nitrogen-containing compounds in water becomes a popular problem for the study of scholars. Among them, the anammox process is favored by most scholars because of its excellent energy saving effect.
The anaerobic ammonia oxidation process is a novel biological denitrification process which can simultaneously convert ammonia nitrogen and nitrite nitrogen into nitrogen by utilizing Anammox bacteria. Successful start-up and operation of the anammox process requires the consumption of large amounts of nitrite. How to obtain nitrite continuously and stably is still a difficult problem which is difficult to solve, and the problem of nitrite sources is solved by two processes of short-cut nitrification and short-cut denitrification in the existing engineering test. Because the short-cut nitrification applied to domestic sewage at present is difficult to maintain for a long time, and the influent water is often accompanied with that organic matters are not fully utilized to inhibit the subsequent anaerobic ammonia oxidation, the short-cut denitrification provides nitrite nitrogen for the anaerobic ammonia oxidation to be a good choice.
The short-cut denitrification process controls the denitrification process in the stage of generating the nitrite, namely, the denitrifying bacteria are prevented from utilizing the carbon source to further reduce the nitrite into nitrogen. Compared with short-cut nitrification, the short-cut denitrification has the advantages of effectively treating nitrate wastewater, fully utilizing organic matters in raw water and the like, and can realize synchronous removal of the nitrate wastewater and domestic sewage. However, the current research shows that the enrichment of the short-range denitrifying bacteria is difficult, the selectivity to carbon sources is strong, easily degradable organic matters are easily utilized, and the difficultly degradable organic matters are difficult to utilize.
Domestic sewage in China generally has the characteristics of low carbon-nitrogen ratio and mostly refractory organic matters, and the shortage of available carbon sources in the domestic sewage results in low short-range denitrification efficiency and high total nitrogen in effluent, so that the short-range denitrification efficiency is difficult to reach the first-level A standard, and the operation cost is increased by adding a simple carbon source for deep denitrification. Therefore, how to more effectively utilize organic matters in the domestic sewage as a carbon source to realize the short-cut denitrification is a problem worthy of research.
In recent years, researchers have found that redox mediators can greatly accelerate the activity of anaerobic microorganisms. Redox mediators are compounds that accelerate the transfer of electrons from the initial electron donor to the final electron acceptor, and can increase the redox reaction rate by one to several orders of magnitude. The related documents report that anthraquinone, as a redox mediator, can improve the activity of nitrate reductase and the activity of nitrite reductase, and has better promotion effect on key enzyme of nitrate reductase. The research shows that the concentration of anthraquinone in 25-75 μmol/L has the promotion effect on the accumulation of nitrite.
The method for starting the short-range denitrification by using the organic matters in the domestic sewage as the carbon source by adding the anthraquinone has the advantages of no need of an additional carbon source, realization of continuous and stable nitrite source and the like, and achieves the aim of synchronously removing the nitrate wastewater and the domestic sewage.
Disclosure of Invention
Compared with the traditional nitrification/denitrification process, the short-cut denitrification-anaerobic ammonia oxidation technology saves the aeration amount and the carbon source, and is a resource-saving and environment-friendly denitrification process. The invention provides a method for starting short-cut denitrification by taking organic matters in domestic sewage as a carbon source by adding anthraquinone, which can fully utilize the organic matters in the domestic sewage, does not need an additional carbon source, saves the cost and can stably obtain nitrite. The method can provide reference for engineering application of short-range denitrification-anaerobic ammonia oxidation.
A device for starting short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as a carbon source is characterized by comprising a nitrate wastewater inlet tank (1), a domestic sewage inlet tank (2), a short-cut denitrification reactor (3), a water outlet tank (4) and an automatic control platform (5); the nitrate wastewater inlet tank (1) is connected with the short-cut denitrification reactor (3) through a first inlet pump (3-1); the domestic sewage inlet tank (2) is connected with the short-cut denitrification reactor (3) through a second inlet pump (3-2); the electric valve (3-3) for draining the short-cut denitrification reactor (3) is connected with the water outlet tank (4).
The short-cut denitrification reactor (3) is internally provided with a drainage electric valve (3-3), a stirring device (3-4), a DO on-line monitor (3-5) and a mud valve (3-6).
The first water inlet pump (3-1), the second water inlet pump (3-2), the electric drainage valve (3-3), the stirrer (3-4) and the DO online monitor (3-5) are connected with the automatic control platform (5).
The device and the method for starting the short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as a carbon source are characterized by comprising the following steps: the periodic operation comprises the following steps:
1) and (3) starting a system: inoculating sludge rich in denitrifying bacteria in a short-cut denitrification reactor; the sludge concentration of the reactor after inoculation is controlled to be 2000-8000mg/L
2) Determining the input of acid salt wastewater and domestic sewage: organic matters in the domestic sewage are used as an organic carbon source, and the nitrate wastewater and the domestic sewage are pumped into the short-range denitrification reactor according to a certain proper carbon-nitrogen ratio. Determining the input amount of the nitrate wastewater and the domestic sewage according to the calculation formulas (1) and (2):
V1+V2＝V*ρ(2)
note: COD-organic matter content in domestic sewage;
V1-domestic sewage intake;
V2-intake of nitrate solution;
v-effective volume of reactor;
rho-drainage ratio;
C/N-ratio of organic matter to nitrate in the water inlet tank, and the set value is selected within the range of 3-6;
3) the runtime adjustment operation is as follows:
pumping the nitrate wastewater inlet tank (1) into the short-cut denitrification reactor (3) through a first inlet pump (3-1) before each period begins; the domestic sewage inlet tank (2) is pumped into the short-cut denitrification reactor (3) through a second inlet pump (3-2); then, an anthraquinone reagent is added into the short-range denitrification reactor (3) to ensure that the concentration of anthraquinone in the reactor is 25-75 mu mol/L, anoxic stirring is started, stirring is stopped until the concentration of nitrate nitrogen is less than 3mg/L or the accumulation rate of nitrite is more than 75%, precipitation and drainage are started, and the next period is started after the reactor is idle.
The invention relates to a device and a method for starting short-cut denitrification by taking organic matters in domestic sewage as a carbon source by adding anthraquinone. The invention has the following advantages:
1) anthraquinone is added to start a short-cut denitrification process taking organic matters in the domestic sewage as a carbon source, so that the organic carbon source in the domestic sewage is fully utilized, deep denitrification is realized, an external carbon source is not required, and the energy consumption of sewage treatment is reduced;
2) the production of nitrite in the nitrate reduction process can be stably and continuously realized, and an electron acceptor is provided for the subsequent anaerobic ammonia oxidation process;
3) organic matters in the domestic sewage are used for short-range denitrification, so that the inhibition of the organic matters on anaerobic ammoxidation can be avoided, and the stability of the subsequent anaerobic ammoxidation process is facilitated.
Drawings
FIG. 1 is a schematic diagram of the structure of a short-cut denitrification-anammox apparatus;
in the figure: 1-nitrate wastewater inlet tank; 2-domestic sewage inlet tank; 3-a short-cut nitrification reactor; 3-1-a first water inlet pump; 3-2-a second water inlet pump; 3-3-electric drainage valve; 3-4-a stirrer; 3-5-DO on-line monitor; 3-6-mud valve; 4-water outlet tank; and 5, automatically controlling the platform.
Detailed Description
The invention is further illustrated by the following figures and examples: as shown in fig. 1: a device and a method for starting short-cut denitrification by adding anthraquinone and taking organic matters in domestic sewage as a carbon source are characterized by comprising a nitrate wastewater inlet tank (1), a domestic sewage inlet tank (2), a short-cut denitrification reactor (3), a water outlet tank (4) and an automatic control platform (5); the nitrate wastewater inlet tank (1) is connected with the short-cut denitrification reactor (3) through a first inlet pump (3-1); the domestic sewage inlet tank (2) is connected with the short-cut denitrification reactor (3) through a second inlet pump (3-2); the electric valve (3-3) for draining the short-cut denitrification reactor (3) is connected with the water outlet tank (4).
The short-cut denitrification reactor (3) is internally provided with a drainage electric valve (3-3), a stirring device (3-4), a DO on-line monitor (3-5) and a mud valve (3-6).
The first water inlet pump (3-1), the second water inlet pump (3-2), the electric drainage valve (3-3), the stirrer (3-4) and the DO online monitor (3-5) are connected with the automatic control platform (5).
In the experimental process, the specific experimental water is taken from domestic sewage of family district of Beijing university of industry and effluent of a nitration reactor, and the specific water quality is as follows: COD concentration is 70-200mg/L, NH4 +-N concentration of 0-2mg/L, NO2 -Concentration of-N<1mg/L，NO3 -the-N is 10-30mg/L, and the pH value is 7.3-7.6. The experimental system is shown in figure 1, the reactor is made of organic glass, and the effective volume of the short-range denitrification reactor (2) is 10L.
The specific operation is as follows:
1) and (3) starting a system: inoculating sludge rich in denitrifying bacteria in a short-cut denitrification reactor; the sludge concentration of the reactor after inoculation is controlled to be 2000-8000mg/L
2) Determining the input of acid salt wastewater and domestic sewage: organic matters in the domestic sewage are used as an organic carbon source, and the nitrate wastewater and the domestic sewage are pumped into the short-range denitrification reactor according to a certain proper carbon-nitrogen ratio. Determining the input amount of the nitrate wastewater and the domestic sewage according to the calculation formulas (1) and (2):
V1+V2＝V*ρ(2)
note: COD-organic matter content in domestic sewage;
V1-domestic sewage intake;
V2-intake of nitrate solution;
v-effective volume of reactor;
rho-drainage ratio;
C/N-ratio of organic matter to nitrate in the water inlet tank, and the set value is selected within the range of 3-6;
3) the runtime adjustment operation is as follows:
pumping the nitrate wastewater inlet tank (1) into the short-cut denitrification reactor (3) through a first inlet pump (3-1) before each period begins; the domestic sewage inlet tank (2) is pumped into the short-cut denitrification reactor (3) through a second inlet pump (3-2); then, an anthraquinone reagent is added into the short-range denitrification reactor (3) to ensure that the concentration of anthraquinone in the reactor is 25-75 mu mol/L, anoxic stirring is started, stirring is stopped until the concentration of nitrate nitrogen is less than 3mg/L or the accumulation rate of nitrite is more than 75%, precipitation and drainage are started, and the next period is started after the reactor is idle.
Average COD, NH of the effluent during the run of the experiment4 +-N,NO3 --N,NO2 -The N, TN concentrations are respectively as follows: 37.04mg/L, 1.02mg/L, 7.28mg/L, 0.68mg/L and 8.56mg/L, and the water outlet indexes are stable and all reach the national first-class A standard.
Claims (2)
1. The device for starting the short-range denitrification by adding anthraquinone and taking organic matters in the domestic sewage as a carbon source is characterized by comprising a nitrate wastewater inlet tank (1), a domestic sewage inlet tank (2), a short-range denitrification reactor (3), a water outlet tank (4) and an automatic control platform (5); the nitrate wastewater inlet tank (1) is connected with the short-cut denitrification reactor (3) through a first inlet pump (3-1); the domestic sewage inlet tank (2) is connected with the short-cut denitrification reactor (3) through a second inlet pump (3-2); a drainage electric valve (3-3) of the short-cut denitrification reactor (3) is connected with a water outlet tank (4);
a drainage electric valve (3-3), a stirring device (3-4), a DO on-line monitor (3-5) and a mud valve (3-6) are arranged in the short-cut denitrification reactor (3);
the first water inlet pump (3-1), the second water inlet pump (3-2), the electric drainage valve (3-3), the stirrer (3-4) and the DO online monitor (3-5) are connected with the automatic control platform (5);
2. the method for carrying out short-cut denitrification by using the device of claim 1 is characterized by comprising the following steps:
1) and (3) starting a system: inoculating sludge containing denitrifying bacteria in a short-cut denitrification reactor; the sludge concentration of the reactor after inoculation is controlled to be 2000-8000 mg/L;
2) determining the input amount of nitrate wastewater and domestic sewage: using organic matters in the domestic sewage as an organic carbon source, and pumping the nitrate wastewater and the domestic sewage into a short-distance denitrification reactor according to a set carbon-nitrogen ratio; determining the input amount of the nitrate wastewater and the domestic sewage according to the calculation formulas (1) and (2):
V1+V2＝V*ρ(2)
note: COD-organic matter content in domestic sewage;
V1-domestic sewage intake;
V2-nitrate wastewater input;
v-effective volume of reactor;
rho-drainage ratio;
C/N-the ratio of the concentration of the organic matters in the water inlet tank to the concentration of the nitrate, wherein the set value is selected within the range of 3-6;
3) the runtime adjustment operation is as follows:
pumping the nitrate wastewater inlet tank (1) into the short-cut denitrification reactor (3) through a first inlet pump (3-1) before each period begins; the domestic sewage inlet tank (2) is pumped into the short-cut denitrification reactor (3) through a second inlet pump (3-2); then, an anthraquinone reagent is added into the short-range denitrification reactor (3) to ensure that the concentration of anthraquinone in the reactor is 25-75 mu mol/L, anoxic stirring is started, stirring is stopped until the concentration of nitrate nitrogen is less than 3mg/L or the accumulation rate of nitrite is more than 75%, precipitation and drainage are started, and the next period is started after the reactor is idle.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112961124A (en) * | 2021-02-19 | 2021-06-15 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
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2020
- 2020-09-23 CN CN202011013336.3A patent/CN112250171A/en active Pending
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|---|---|---|---|---|
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| CN103952445A (en) * | 2014-04-14 | 2014-07-30 | 杭州电子科技大学 | Method for promoting production of fermentation acid from remaining sludge by utilizing redox mediator |
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| CN107253762A (en) * | 2017-07-05 | 2017-10-17 | 扬州大学 | A kind of short-cut denitrification dephosphorization couples the quick start method of Anammox |
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| Title |
|---|
| 尹鑫等: "氧化还原介体对短程反硝化细菌亚硝氮积累影响研究", 《江西农业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112961124A (en) * | 2021-02-19 | 2021-06-15 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
| CN112961124B (en) * | 2021-02-19 | 2022-06-21 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
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