CN112245436A - Application of 18 beta-glycyrrhetinic acid in preparation of product for enhancing anti-fatigue capability of organism - Google Patents
Application of 18 beta-glycyrrhetinic acid in preparation of product for enhancing anti-fatigue capability of organism Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention belongs to the field of health-care food, and particularly relates to application of 18 beta-glycyrrhetinic acid in preparation of a product for enhancing the anti-fatigue capability of an organism. The 18 beta-glycyrrhetinic acid has the effects of remarkably enhancing exercise endurance and delaying the occurrence of exercise-induced fatigue. The anti-fatigue food ingredients have the effect after being taken for a short time, and can enhance the exercise endurance of mice, prolong the exercise time and increase the exercise distance after being taken 2.5 hours before the exhaustion exercise. The invention provides a new component for developing anti-fatigue health-care food and has important application value.
Description
Technical Field
The invention belongs to the field of health-care food, and particularly relates to application of 18 beta-glycyrrhetinic acid in preparation of a product for enhancing the anti-fatigue capability of an organism.
Background
Fatigue is a phenomenon that is commonly found in the body, mediated by a complex series of physiological and biochemical processes. Fatigue is defined as the weakening of the force that a muscle can produce such that it cannot maintain a predetermined strength of movement, i.e. exercise fatigue. The generation of sports fatigue is often accompanied by changes in energy substances, metabolites, hormone levels, cellular metabolism regulating enzymes, antioxidant system enzymes and the like, and the essential reason of the generation is that the body feels uncomfortable due to the imbalance of homeostasis. The persistent fatigue or the severe fatigue not only affects the normal life of people, but also causes endocrine disturbance, immunity reduction and even organic diseases, thus threatening the health. The sports fatigue is caused by the crowds of over-trained athletes, fire-fighting officers and soldiers who continuously work at high intensity, medical staff, scientific research staff, labor people and the like. In addition, the general population may also often feel tired. Therefore, the development of anti-fatigue products suitable for the vast population has important and profound significance.
The functional food for resisting sports fatigue on the market contains various ingredients, and the ingredients are mainly divided into two main categories. One is basic substance required by body movement, such as saccharide, amino acid, inorganic salt, etc.; one is a functional factor based on its molecular regulation mechanism, which has the functions of increasing energy utilization rate, eliminating harmful metabolites (such as ROS), relieving body fatigue, etc., such as carnosine, carnitine, caffeine, ginsenoside, etc. Wherein the anti-fatigue action mechanism of part of substances relates to a nerve regulation mechanism and is suspected to be excitant; the anti-fatigue efficacy of some substances remains to be further verified; and part of the substances are expensive and are not suitable for consumption of the masses. Furthermore, most of the anti-fatigue substances found require long-term supplementation for at least 1 month to exert their effective effect.
Disclosure of Invention
One of the purposes of the invention is to provide a new application of 18 beta-glycyrrhetinic acid in the field of health-care food.
The invention provides a new application of 18 beta-glycyrrhetinic acid in the field of health food, which comprises the following steps: application of 18 beta-glycyrrhetinic acid as an anti-fatigue component in preparing a product for enhancing the anti-fatigue capability of an organism.
The product can be medicine, food, health food, etc.
The anti-fatigue capability may specifically be an anti-exercise fatigue capability.
When the 18 beta-glycyrrhetinic acid is used as an anti-fatigue component, the 18 beta-glycyrrhetinic acid is taken 2.5 hours before the start of exhaustion exercise and is taken once a day.
When the 18 beta-glycyrrhetinic acid is used as an anti-fatigue component, the anti-fatigue basic formula can be added to prepare powder, tablets, capsules and the like, and the anti-fatigue basic formula can also be added to food, such as biscuits, rice milk, milk powder, sesame paste and the like.
Another object of the present invention is to provide a health food capable of enhancing the anti-fatigue ability of the body.
The health food capable of enhancing the anti-fatigue capability of the organism provided by the invention takes 18 beta-glycyrrhetinic acid as an effective component.
The fatigue may specifically be sports fatigue.
The health food containing 18 beta-glycyrrhetinic acid as effective component and capable of enhancing body fatigue resistance is administered 2.5h before the start of exhaustion exercise, and is administered once per day.
The invention also provides a method for enhancing the anti-fatigue capability of the organism.
The method comprises the following steps: the preparation is administered once a day, and 18 beta-glycyrrhetinic acid or health food containing 18 beta-glycyrrhetinic acid as effective component and capable of enhancing fatigue resistance is administered 2.5h before the start of exhaustion exercise.
The liquorice is a common traditional Chinese medicine, and the 2015 edition of Chinese pharmacopoeia shows that the part of the liquorice used as the medicine is dry roots and rhizomes. Glycyrrhizic acid and glycyrrhetinic acid, which are the main bioactive components of the root, are considered to have a liver-protecting effect. After oral administration, glycyrrhizic acid is totally decomposed into 18 β -glycyrrhetinic acid in the small intestine by intestinal bacteria.
According to the pharmacopoeia, about 400mg of 18 beta-glycyrrhetinic acid is safe to be taken orally every day. The dosage of the product is safe according to the conversion formula, namely the daily oral dosage of a human body is the mouse dosage (mg/kg)/9.1 x 60.
Research shows that glycyrrhetinic acid has the effects of resisting oxidation, resisting inflammation, regulating glycolipid metabolism, protecting liver and the like. The research shows that the 18 beta-glycyrrhetinic acid has the function of resisting fatigue.
Experiments prove that the anti-sports fatigue food component can have the potential effects of prolonging endurance exercise and accelerating the exercise endurance of mice in a high-strength accelerated exercise fatigue model after being supplemented for 3 days continuously, the exercise time is prolonged by 7 percent (P is 0.007), the exercise distance is increased by 29 percent (P is 0.023), and speed setting and experimental results are shown in figures 1-3.
Experiments prove that the anti-sports fatigue food ingredients can effectively prolong the sports endurance of a mouse in a high-strength accelerated sports fatigue model after being supplemented for 3 days continuously, the percentage of the lengthening of the sports time is 26 percent (P is 0.034), the percentage of the increasing of the sports distance is 37 percent (P is 0.034), and the speed setting and the experimental results are shown in figures 4-6.
Experiments prove that the anti-sports fatigue food ingredient can effectively protect muscle damage caused by high-intensity sports after being supplemented for 3 days continuously, the creatine kinase reduction percentage is 38% (P is 0.015), and the creatine kinase basically falls back to the resting level, and the experimental results are shown in figure 7.
The food ingredients are scientifically and reasonably screened out the food-borne functional ingredients with the anti-fatigue effect according to the existing fatigue generation mechanism, such as the energy exhaustion theory, the metabolite accumulation theory, the internal environment homeostasis disorder theory of the organism, the oxygen free radical theory, the central fatigue and the like. It has the characteristics of naturalness, safety, high efficiency, no toxic or side effect, clear action way and the like. The product has effect in enhancing anti-fatigue ability after short-term administration, and can be administered 2.5 hr before the start of exhaustion exercise to enhance exercise endurance of mouse, prolong exercise time, and increase exercise distance.
The invention aims to provide a food component which has wide raw material source, high price, short supplement time and excellent anti-fatigue effect, and provides a new choice for the research of anti-fatigue formula products.
Drawings
Fig. 1 is a graph showing the speed and time settings of the endurance exercise + high-intensity accelerated exercise fatigue model in example 1 of the present invention.
FIG. 2 shows the effect of supplementing 18 β -glycyrrhetinic acid for 3 days on the motility time of mice in a resistance exercise + high-intensity accelerated exercise fatigue model.
FIG. 3 shows the effect of supplementing 18 β -glycyrrhetinic acid for 3 days on the distance to exercise exhaustion in mice in a resistance exercise + high-intensity accelerated exercise fatigue model.
Fig. 4 is a graph of the speed and time settings of the high intensity accelerated motion fatigue model in example 2 of the present invention.
FIG. 5 shows the effect of 3 days supplementation with 18 β -glycyrrhetinic acid on the time to exhaustion of locomotion in mice in a high-intensity accelerated exercise fatigue model.
FIG. 6 shows the effect of 3 days supplementation with 18 β -glycyrrhetinic acid on the time to exhaustion of locomotion in mice in a high-intensity accelerated exercise fatigue model.
FIG. 7 shows the effect of supplementation with 18 β -glycyrrhetinic acid for 3 days on creatine kinase in mouse serum for the same time period of exercise in a high intensity accelerated exercise fatigue model.
Detailed Description
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified.
Example 1
The 18 beta-glycyrrhetinic acid can relieve the sports fatigue of a mouse in a resistance sports plus high-strength accelerated sports fatigue model.
The test method comprises the following steps: male C57 BL/6J mice, 36, 6-8 weeks old, all mice were randomly assigned to the resting group (12), model group (12), formula group (12). By adopting a stomach filling method, a resting group and a model group are filled with 0.1 percent of sodium carboxymethylcellulose every day, a formula group is filled with 25mg/kg bw of 18 beta-glycyrrhetinic acid every day, and a solvent is 0.1 percent of sodium carboxymethylcellulose. 8 in the morning: gavage is performed 30 times for 1 time. The supplementation was continued for 3 days, and 2.5 hours after the last supplementation, the motility exhaustion time of the mice was measured while the movement distance was calculated.
And (3) motion model: the experimental animal runs the platform. The gradient is 0 degree, the initial speed is 10m/min, 2m/min is added every 15min until 14m/min, the speed is accelerated to 20m/min after 15min, the speed is increased to 22m/min after 5min, 1m/min is added to 24m/min every 5min, and the running is continued until the vigor is exhausted. The movement speed and time settings are shown in figure 1.
The force criteria were set such that the mouse stayed on the electrodes for more than 1 minute and was still unable to run forward with a brush gently stimulating its tail or legs.
The experimental results (fig. 2 and 3) show that the anti-exercise fatigue food component is continuously supplemented for 3 days, has the potential effect of prolonging the exercise endurance and the exercise endurance of the mice in the high-intensity accelerated exercise fatigue model, the exercise time is prolonged by 7 percent (P is 0.007), and the exercise distance is increased by 29 percent (P is 0.023).
Example 2
The 18 beta-glycyrrhetinic acid can relieve the sports fatigue of the mouse in a high-strength accelerated sports fatigue model.
The test method comprises the following steps: male C57 BL/6J mice, 36, 6-8 weeks old, all mice were randomly assigned to the resting group (12), model group (12), administration group (12). By adopting a stomach filling method, a resting group and a model group are filled with 0.1 percent of sodium carboxymethylcellulose every day, an administration group is filled with 25mg/kg bw of 18 beta-glycyrrhetinic acid every day, and the solvent is 0.1 percent of sodium carboxymethylcellulose. 8 in the morning: gavage is performed 30 times for 1 time. The supplementation was continued for 3 days, and 2.5 hours after the last supplementation, the motility exhaustion time of the mice was measured while the movement distance was calculated.
And (3) motion model: the experimental animal runs the platform. The slope is 0 degree, the initial speed is 5m/min, 5m/min is added every 5min, and after the speed is increased to 25m/min, the running is continued until the exhaustion. The movement speed and time settings are shown in figure 4.
The force criteria were set such that the mouse stayed on the electrodes for more than 10 seconds and was still unable to run forward with a brush gently stimulating its tail or legs.
The results of the experiment (fig. 5 and 6) show that the anti-exercise fatigue food ingredient is continuously supplemented for 3 days, and has the potential effect of prolonging the exercise endurance of the mice in the high-intensity accelerated exercise fatigue model, the exercise time is prolonged by 26 percent (P ═ 0.034), and the exercise distance is increased by 37 percent (P ═ 0.034).
The results of comparative example 1 and example 2 show that 18 β -glycyrrhetinic acid has a stronger anti-fatigue effect in the high-intensity accelerated exercise fatigue model than in the endurance exercise + high-intensity accelerated exercise fatigue model.
Example 3
Alleviation of muscle damage in mice in a high-intensity accelerated exercise fatigue model by 18 β -glycyrrhetinic acid.
The test method comprises the following steps: male C57 BL/6J mice, 36, 6-8 weeks old, all mice were randomly assigned to the resting group (12), model group (12), administration group (12). By adopting a stomach filling method, a resting group and a model group are filled with 0.1 percent of sodium carboxymethylcellulose every day, an administration group is filled with 25mg/kg bw of 18 beta-glycyrrhetinic acid every day, and the solvent is 0.1 percent of sodium carboxymethylcellulose. 8 in the morning: gavage is performed 30 times for 1 time. Continuously supplementing for 3 days, uniformly running for 20 minutes 2.5 hours after the last supplementation, taking blood, and measuring the content of creatine kinase in the blood serum of the mice.
And (3) motion model: the experimental animal runs the platform. The slope is 0 degree, the initial speed is 5m/min, 5m/min is added every 5min, and after the speed is increased to 25m/min, the running is continued until the exhaustion. The movement speed and time settings are shown in figure 4.
The force criteria were set such that the mouse stayed on the electrodes for more than 10 seconds and was still unable to run forward with a brush gently stimulating its tail or legs.
The experimental result (figure 7) shows that the continuous supplementation of the anti-sports fatigue food ingredient for 3 days can obviously reduce the increased serum creatine kinase content of the mice in a certain time of high-intensity accelerated exercise process, the reduction percentage is 38 percent (P ═ 0.015), and the creatine kinase basically falls back to the resting level, which indicates that the 18 beta-glycyrrhetinic acid has the function of delaying sports fatigue.
Comparative example 1
The existing literature indicates that resveratrol has the function of relieving the sports injury of mice in a high-strength accelerated sports fatigue model, but the relieving degree is not as good as that of 18 beta-glycyrrhetinic acid.
The test method comprises the following steps: male Wistar rats, 8 weeks old, were divided into resting group, model group, administration group. By adopting a gastric lavage method, the administration group is gavaged with 10mg/ml of resveratrol every day, and the solvent is a neutral aqueous solution. The stomach was perfused continuously for 5 weeks and run out experiments were performed.
And (3) motion model: the experimental animal runs the platform. The slope is 10 degrees, the speed is 25m/min, and the running is performed till exhaustion.
The force criteria were set such that the mouse stayed on the electrodes for more than 10 seconds and was still unable to run forward with a brush gently stimulating its tail or legs.
The experimental result shows that the continuous supplementation of resveratrol for 5 weeks can obviously reduce the creatine kinase level of mice after exercise, but cannot recover to the creatine kinase level of mice in a resting state, and the effect of veratryl alcohol on relieving injury caused by high-intensity exercise is not as good as that of 18 beta-glycyrrhetinic acid.
Reference documents:
Vafaee R,Soori H,Hedayati M,Ainy E,Hatamabadi H.Effects of resveratrol supplementation in male Wistar rats undergoing an endurance exercise and acute exercise training.Hum Antibodies.2019；27(4):257-264.doi:10.3233/HAB-190380.PMID:31127758.
Claims (9)
1.18 beta-glycyrrhetinic acid is used as an anti-fatigue component in the preparation of products for enhancing the anti-fatigue capability of organisms.
2. Use according to claim 1, characterized in that: the anti-fatigue capability is the capability of resisting sports fatigue.
3. Use according to claim 1 or 2, characterized in that: the product is a medicine, a food or a health food.
4. Use according to any one of claims 1-3, characterized in that: when the 18 beta-glycyrrhetinic acid is used as an anti-fatigue component, the 18 beta-glycyrrhetinic acid is taken 2.5 hours before the start of exhaustion exercise and is taken once a day.
5. Use according to any one of claims 1 to 4, characterized in that: when the 18 beta-glycyrrhetinic acid is used as an anti-fatigue component, the anti-fatigue basic formula can be added to prepare powder, tablets and capsules, and the anti-fatigue basic formula can also be added to food, wherein the food can be biscuit, rice milk, milk powder and sesame paste.
6. A health food for enhancing anti-fatigue ability of organism contains 18 beta-glycyrrhetinic acid as effective component.
7. The health food according to claim 6, wherein: the fatigue is sports fatigue.
8. The health food according to claim 6 or 7, wherein: the health food containing 18 beta-glycyrrhetinic acid as effective component and capable of enhancing body fatigue resistance is taken 2.5h before the start of exhaustion exercise, and is taken once a day.
9. A method for enhancing the anti-fatigue capability of a body comprises the following steps: administering 18 β -glycyrrhetinic acid or the health food according to any one of claims 6 to 8 once a day 2.5h before the start of exhaustion exercise of the body.
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