CN112226346B - Cell mycoplasma detecting instrument based on pcr technology - Google Patents
Cell mycoplasma detecting instrument based on pcr technology Download PDFInfo
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- CN112226346B CN112226346B CN202011115224.9A CN202011115224A CN112226346B CN 112226346 B CN112226346 B CN 112226346B CN 202011115224 A CN202011115224 A CN 202011115224A CN 112226346 B CN112226346 B CN 112226346B
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- 241000204031 Mycoplasma Species 0.000 title claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 238000005070 sampling Methods 0.000 claims description 80
- 229920000742 Cotton Polymers 0.000 claims description 63
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 46
- 229910052742 iron Inorganic materials 0.000 claims description 23
- 238000001514 detection method Methods 0.000 claims description 12
- 230000006698 induction Effects 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 230000000087 stabilizing effect Effects 0.000 claims description 4
- 230000002452 interceptive effect Effects 0.000 claims description 2
- 230000005484 gravity Effects 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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Abstract
The invention discloses a cell mycoplasma detecting instrument based on pcr technology, which comprises a box body, wherein an empty bin is arranged in the box body, a stable clamping mechanism is arranged in the empty bin, the stable clamping mechanism comprises a stable plate, two clamping blocks are fixedly connected to the stable plate, the two clamping blocks are symmetrical front and back by taking the center line of the stable plate as the center, and each clamping block is in sliding connection with a sliding chute.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a mycoplasma cellularis detection instrument based on a pcr technology.
Background
The development of cell detection technology in recent years is very rapid, cell detection is more intuitive to find the root cause of some physical symptoms, or the health degree of cells is checked to predict some diseases in time, and the like, so that great convenience is brought to the current life.
Disclosure of Invention
The invention aims to solve the technical problem of providing a cell mycoplasma detection instrument based on pcr technology, and solves the problems of single function and defects of the cell detection instrument.
The invention is realized by the following technical scheme.
The invention relates to a cell mycoplasma detecting instrument based on pcr technology, which comprises a box body, wherein an empty bin is arranged in the box body, a stable clamping mechanism is arranged in the empty bin, the stable clamping mechanism comprises a stable plate, two clamping blocks are fixedly connected to the stable plate, the two clamping blocks are symmetrical front and back by taking the center line of the stable plate as the center, each clamping block is in sliding connection with a sliding groove, the sliding grooves are formed in the box body, the lower end face of the stable plate is fixedly connected with a clamping plate, the lower end face of the stable plate is fixedly connected with a lifting rod, the lifting rod is in sliding connection with a lifting bin, the lifting bin is arranged in the lifting bin, the lifting bin is fixedly connected with the inner wall of the lower side of the empty bin, a lifting spring is further arranged in the lifting bin, the lower end of the lifting spring is fixedly connected to the inner wall of the lower side of the lifting bin, and the upper end of the lifting spring is fixedly connected to the lifting rod, a fixed plate is fixedly connected to the left end face of the box body, an induction bin with an upward opening is arranged in the fixed plate, a first pressure sensor is arranged in the induction bin, a clamping spring is fixedly connected to the upper end face of the first pressure sensor, a clamping soft body is fixedly connected to the upper end of the clamping spring and is in sliding connection with the inner wall of the induction bin, the face of a person to be sampled is placed on the fixed plate, and the clamping plate stably clamps the face along with the descending of the stabilizing plate to wait for the next operation;
a cotton swab sampling mechanism is further arranged in the empty bin and comprises a movable plate, the movable plate is fixedly connected to the inner wall of the lower side of the empty bin, a movable groove is formed in the movable plate and is connected with a movable clamping block in a sliding mode, the movable clamping block is fixedly connected to a first hydraulic machine, the first hydraulic machine is connected with the left end face of the movable plate in a sliding mode, the left end face of the first hydraulic machine is connected with a first hydraulic rod in an interactive mode, the left end of the first hydraulic rod is fixedly connected to the sampling plate, the inner wall of the lower side of the empty bin is fixedly connected with a second hydraulic machine, the upper end face of the second hydraulic machine is connected with a third hydraulic rod in a sliding mode, the third hydraulic rod is connected with a guide plate in a sliding mode, the guide plate is fixedly connected to the inner wall of the lower side of the empty bin, and the upper end of the third hydraulic rod is fixedly connected to the hydraulic plate, the hydraulic plate is fixedly connected to the lower end of a movable plate, two movable clamping blocks are arranged on the movable plate and are connected with the movable plate in a sliding mode, the movable clamping blocks are fixedly connected to the lower end face of the sampling plate, a rotating shaft is connected to the sampling plate in a rotating mode, a blanking shaft is fixedly connected to the rotating shaft, a pushing bin is arranged below the blanking shaft, a fifth electromagnet is arranged in the pushing bin and is fixedly connected to the inner wall of the right side of the pushing bin, a pushing spring is fixedly connected to the left end of the fifth electromagnet, the left end of the pushing spring is fixedly connected to the pushing plate, the pushing plate is connected with the inner wall of the pushing bin in a sliding mode, a sliding plate is further arranged in the pushing bin and is connected with the sliding bin in a sliding mode, the sliding bin is arranged in the sampling plate, and a second pressure sensor is further arranged in the sliding bin, the second pressure sensor is fixedly connected to the lower inner wall of the sliding bin, the upper end of the second pressure sensor is fixedly connected with a sliding spring, the upper end of the sliding spring is fixedly connected with the sliding plate, the sliding plate is fixedly connected with a supporting rod, the right end of the supporting rod is fixedly connected with a conductive iron block, the conductive iron block can be in contact with two conductive blocks, the two conductive blocks are symmetrical front and back by taking the center line of the conductive iron block as the center, the conductive blocks are fixedly connected to the lower inner wall of the circuit bin, the circuit bin is arranged in the sampling plate, the conductive block at the rear side is connected with a first annunciator through an electric wire, the first annunciator is fixedly connected to the lower inner wall of the circuit bin, the first annunciator is connected with a power supply through an electric wire, the power supply is fixedly connected to the lower inner wall of the circuit bin, and the power supply is connected with the second annunciator through an electric wire, the second annunciator is fixedly connected to the lower inner wall of the circuit bin, the second annunciator is connected with the conductive block on the front side through an electric wire, the sampling plate is internally provided with a driving bin, the driving bin is internally provided with a fourth electromagnet, the fourth electromagnet is fixedly connected to the upper inner wall of the driving bin, the lower end face of the fourth electromagnet is fixedly connected with a driving spring, the lower end of the driving spring is fixedly connected with a driving block, the driving block is in sliding connection with the inner wall of the driving bin, the lower end of the driving block extends into a rotating bin, the rotating bin is arranged in the sampling plate, the lower end of the driving block is in contact with a cotton swab fixing pipe, the cotton swab fixing pipe is fixedly connected with two rotating shafts, the two rotating shafts are symmetrical around the central line of the driving block, one end of the rotating shaft, which is far away from the driving block, is rotatably connected with the inner walls on the front side and the rear side of the rotating bin, a piston bin is arranged in the cotton swab fixing pipe, a second electromagnet is arranged in the piston bin, the second electromagnet is fixedly connected in the piston bin, a piston spring is fixedly connected to the front end surface and the rear end surface of the second electromagnet respectively, the two piston springs are symmetrical front and back by taking the center line of the second electromagnet as the center, the other end of each piston spring is fixedly connected with a piston plate, the piston plates are connected with the inner wall of the piston bin in a sliding manner, the two piston plates are mutually contacted, a pipeline is arranged in the cotton swab fixing pipe, a magnetic supporting block is arranged in the pipeline and slides with the inner wall of the supporting bin, the supporting bin is arranged in a supporting bin, the supporting bin is fixedly connected with the cotton swab fixing pipe, a third electromagnet is further arranged in the supporting bin and is fixedly connected to the inner wall of the upper side of the supporting bin, the lower end face of the third electromagnet is fixedly connected with a supporting spring, the other end of the supporting spring is fixedly connected with the magnetic supporting block, the height of the cotton swab fixing pipe is adjusted, a sampling cotton swab falls from the blanking shaft into the pushing bin and is pushed into the pipeline by the pushing plate to be clamped by the magnetic supporting block, and the cotton swab extends into the mouth of a person to be sampled along with the cotton swab fixing pipe under the control of an operator for sampling;
the sample bottling mechanism is also arranged in the empty bin and comprises a chopping box, the chopping box is fixedly connected to the inner wall of the upper side of the empty bin, the chopping bin is arranged in the chopping box, a third hydraulic machine is arranged in the chopping bin, the lower end of the third hydraulic machine is connected with a second hydraulic rod in a sliding manner, the lower end of the second hydraulic rod is fixedly connected with a chopping blade, the chopping blade is connected with the inner wall of the chopping bin in a sliding manner, a sampling bottle is arranged below the chopping blade and is connected with a clamping plate in a sliding manner, the clamping plate is fixedly connected to a fixed block, a sample bin is arranged in the sampling bottle, a third pressure sensor is arranged in the sample bin and is fixedly connected with the inner wall of the sample bin, a sampling spring is fixedly connected to the upper end face of the third pressure sensor, and the upper end of the sampling spring is fixedly connected to a pressure plate, the pressure plate is connected with the inner wall of the sample bin in a sliding mode, the fixing block is fixedly connected to the conveying belt, the chopping blade descends to chop the sampling cotton swabs, the sampling cotton swabs fall into the sampling bottles, and the sampling cotton swabs are conveyed to the next process to be processed under the action of the conveying belt.
Preferably, stabilize fixture still includes the rack, rack fixed connection be in on the right-hand member face of lifter, rack and drive gear meshing, drive gear fixed connection is at the front end of drive pivot, the drive pivot rotates with the third motor to be connected, third motor fixed connection be in on the rear side inner wall of empty storehouse.
Preferably, the cotton swab sampling mechanism further comprises a feeding box, the feeding box is fixedly connected to the upper end wall of the box body, a feeding bin is arranged in the feeding box and faces the blanking shaft, an electronic eye is fixedly connected to the upper end of the cotton swab fixing tube, a display screen is fixedly connected to the upper end of the box body, two ultraviolet disinfectors are fixedly connected to the inner wall of the upper side of the empty bin, the two ultraviolet disinfectors are bilaterally symmetrical with the center line of the empty bin as the center, a second motor is further arranged in the sampling plate and fixedly connected to the sampling plate, a second driving shaft is rotatably connected to the left end face of the second motor, a first bevel gear is fixedly connected to the left end of the second driving shaft and can be meshed with a second bevel gear, and an iron movable plate is rotatably connected to the lower end of the second bevel gear, the iron fly leaf and activity storehouse sliding connection, the activity storehouse is established in the sampling plate, the lower extreme fixedly connected with movable spring of iron fly leaf, on the first electro-magnet of lower extreme fixed connection of movable spring, first electro-magnet with the downside inner wall fixed connection in activity storehouse, second bevel gear also can mesh with third bevel gear, third bevel gear's left end face fixedly connected with the axis of rotation.
Preferably, the sample bottling mechanism further comprises a first motor, the first motor is fixedly connected to the rear end face of the box body, a first driving shaft is rotatably connected to the right end face of the first motor, a conveying wheel is fixedly connected to the first driving shaft, the conveying wheel is in friction transmission with the conveying wheel on the front side through the conveying belt, a limiting rod is further fixedly connected to the rear end face of the box body, a limiting block is fixedly connected to the rear end of the limiting rod, a baffle is further fixedly connected to the conveying belt, a first inductor is embedded in the chopping blade, the first inductor can interact with a second inductor, and the second inductor is embedded in the cotton swab fixing tube.
The invention has the beneficial effects that: the invention can replace the manual sampling step in the original mycoplasma cellularis detection, ensure the safety of sampling personnel, prevent infectious viruses from invading the sampling personnel, avoid the pollution to the sample in the sampling process without the contact operation of personnel, ensure the result of cell detection to be more accurate, improve the working efficiency of cell sampling by replacing manpower with a machine, and save the labor cost.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an enlarged schematic view of the embodiment of the present invention at A in FIG. 1;
FIG. 3 is an enlarged schematic view of the embodiment of the present invention at B in FIG. 1;
FIG. 4 is an enlarged schematic view of the embodiment of the present invention at C in FIG. 1;
FIG. 5 is a schematic view of the embodiment of the present invention in the direction D-D in FIG. 1;
FIG. 6 is a schematic view of the embodiment of the present invention in the direction E-E of FIG. 2;
FIG. 7 is a schematic view of the embodiment of the present invention in the direction F-F in FIG. 1;
FIG. 8 is a schematic view of the embodiment of the present invention in the direction of G-G in FIG. 1;
FIG. 9 is a schematic view of the embodiment of the present invention in the direction H-H in FIG. 2;
FIG. 10 is a schematic view along the direction I-I in FIG. 1 according to an embodiment of the present invention.
Detailed Description
The invention will now be described in detail with reference to fig. 1-10, wherein for ease of description the orientations described below are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
With reference to fig. 1-10, the cell mycoplasma detecting instrument based on pcr technology includes a box 11, an empty bin 12 is disposed in the box 11, a stable clamping mechanism 201 is disposed in the empty bin 12, the stable clamping mechanism 201 includes a stable plate 29, two fixture blocks 109 are fixedly connected to the stable plate 29, the two fixture blocks 109 are symmetrical around a central line of the stable plate 29, each fixture block 109 is slidably connected to a sliding groove 110, the sliding groove 110 is disposed in the box 11, a clamping plate 30 is fixedly connected to a lower end surface of the stable plate 29, a lifting rod 101 is fixedly connected to a lower end surface of the stable plate 29, the lifting rod 101 is slidably connected to a lifting bin 103, the lifting bin 103 is disposed in the lifting box 102, the lifting box 102 is fixedly connected to a lower inner wall of the empty bin 12, a lifting spring 104 is further disposed in the lifting bin 103, the lower end of the lifting spring 104 is fixedly connected to the lower inner wall of the lifting bin 103, the upper end of the lifting spring 104 is fixedly connected to the lifting rod 101, the left end surface of the box body 11 is fixedly connected to a fixing plate 31, an induction bin 32 with an upward opening is arranged in the fixing plate 31, a first pressure sensor 33 is arranged in the induction bin 32, a clamping spring 34 is fixedly connected to the upper end surface of the first pressure sensor 33, the upper end of the clamping spring 34 is fixedly connected to a clamping soft body 35, the clamping soft body 35 is slidably connected with the inner wall of the induction bin 32, a face of a person to be sampled is placed on the fixing plate 31, and the clamping plate 30 stably clamps the face along with the descending of the stabilizing plate 29 to wait for the next operation;
the cotton swab sampling mechanism 202 is further arranged in the empty bin 12, the cotton swab sampling mechanism 202 comprises a movable plate 14, the movable plate 14 is fixedly connected to the inner wall of the lower side of the empty bin 12, a movable groove 16 is arranged in the movable plate 14, the movable groove 16 is slidably connected with a movable clamping block 15, the movable clamping block 15 is fixedly connected to a first hydraulic machine 17, the first hydraulic machine 17 is slidably connected with the left end face of the movable plate 14, a first hydraulic rod 18 is interactively connected to the left end face of the first hydraulic machine 17, the left end of the first hydraulic rod 18 is fixedly connected to a sampling plate 52, a second hydraulic machine 47 is fixedly connected to the inner wall of the lower side of the empty bin 12, a third hydraulic rod 48 is slidably connected to the upper end face of the second hydraulic machine 47, the third hydraulic rod 48 is slidably connected to a guide plate 46, and the guide plate 46 is fixedly connected to the inner wall of the lower side of the empty bin 12, the upper end of the third hydraulic rod 48 is fixedly connected to a hydraulic plate 49, the hydraulic plate 49 is fixedly connected to the lower end of a moving plate 50, the moving plate 50 is provided with two moving clamping blocks 51, the two moving clamping blocks 51 are slidably connected with the moving plate 50, the moving clamping blocks 51 are fixedly connected to the lower end face of the sampling plate 52, the sampling plate 52 is rotatably connected with a rotating shaft 62, the rotating shaft 62 is fixedly connected with a blanking shaft 67, a pushing bin 64 is arranged below the blanking shaft 67, a fifth electromagnet 66 is arranged in the pushing bin 64, the fifth electromagnet 66 is fixedly connected to the inner wall of the right side of the pushing bin 64, the left end of the fifth electromagnet 66 is fixedly connected with a pushing spring 65, the left end of the pushing spring 65 is fixedly connected to a pushing plate 63, and the pushing plate 63 is slidably connected to the inner wall of the pushing bin 64, a sliding plate 78 is further arranged in the pushing bin 64, the sliding plate 78 is slidably connected with a sliding bin 75, the sliding bin 75 is arranged in the sampling plate 52, a second pressure sensor 76 is further arranged in the sliding bin 75, the second pressure sensor 76 is fixedly connected to the inner wall of the lower side of the sliding bin 75, a sliding spring 77 is fixedly connected to the upper end of the second pressure sensor 76, the sliding plate 78 is fixedly connected to the upper end of the sliding spring 77, a supporting rod 74 is fixedly connected to the sliding plate 78, a conductive iron block 73 is fixedly connected to the right end of the supporting rod 74, the conductive iron block 73 can be in contact with two conductive blocks 71, the two conductive blocks 71 are symmetrical front and back with the center line of the conductive iron block 73 as the center, the conductive blocks 71 are fixedly connected to the inner wall of the lower side of the circuit bin 68, and the circuit bin 68 is arranged in the sampling plate 52, the conductive block 71 at the rear side is connected with a first annunciator 70 through an electric wire, the first annunciator 70 is fixedly connected on the lower inner wall of the circuit bin 68, the first annunciator 70 is connected with a power source 69 through an electric wire, the power source 69 is fixedly connected on the lower inner wall of the circuit bin 68, the power source 69 is connected with a second annunciator 72 through an electric wire, the second annunciator 72 is fixedly connected on the lower inner wall of the circuit bin 68, the second annunciator 72 is connected with the conductive block 71 at the front side through an electric wire, a driving bin 80 is further arranged in the sampling plate 52, a fourth electromagnet 79 is arranged in the driving bin 80, the fourth electromagnet 79 is fixedly connected on the upper inner wall of the driving bin 80, a driving spring 81 is fixedly connected on the lower end face of the fourth electromagnet 79, a driving block 82 is fixedly connected with the lower end face of the driving spring 81, and the driving block 82 is connected with the inner wall of the driving bin 80 in a sliding manner, the lower end of the driving block 82 extends into a rotating chamber 87, the rotating chamber 87 is arranged in the sampling plate 52, the lower end of the driving block 82 is in contact with a cotton swab fixing pipe 83, the cotton swab fixing pipe 83 is fixedly connected with two rotating shafts 86, the two rotating shafts 86 are symmetrical front and back by taking the central line of the driving block 82 as the center, one end, far away from the driving block 82, of each rotating shaft 86 is rotatably connected with the inner walls of the front side and the rear side of the rotating chamber 87, a piston chamber 88 is arranged in the cotton swab fixing pipe 83, a second electromagnet 89 is arranged in the piston chamber 88, the second electromagnet 89 is fixedly connected in the piston chamber 88, the front end face and the rear end face of the second electromagnet 89 are respectively and fixedly connected with a piston spring 90, the two piston springs 90 are symmetrical front and back by taking the central line of the second electromagnet 89 as the center, and the other end of each piston spring 90 is fixedly connected with a piston plate 85, the piston plates 85 are slidably connected with the inner wall of the piston bin 88, the two piston plates 85 are in contact with each other, a pipeline 84 is arranged in the swab fixing pipe 83, a magnetic supporting block 97 is arranged in the pipeline 84, the magnetic supporting block 97 and the supporting bin 94 slide on the inner wall, the supporting bin 94 is arranged in a supporting box 93, the supporting box 93 and the swab fixing pipe 83 are fixedly connected, a third electromagnet 95 is further arranged in the supporting bin 94, the third electromagnet 95 is fixedly connected to the inner wall of the upper side of the supporting bin 94, a supporting spring 96 is fixedly connected to the lower end face of the third electromagnet 95, the other end of the supporting spring 96 is fixedly connected with the magnetic supporting block 97, the height of the swab fixing pipe 83 is adjusted, a sampling swab falls from the blanking shaft 67 to the pushing bin 64 and is pushed by the pushing plate 63 to the pipeline 84 to be clamped by the magnetic supporting block 97, the cotton swab extends into the mouth of a person to be sampled along with the cotton swab fixing tube 83 under the control of an operator for sampling;
the empty bin 12 is further internally provided with a sample bottling mechanism 203, the sample bottling mechanism 203 comprises a chopping box 23, the chopping box 23 is fixedly connected to the inner wall of the upper side of the empty bin 12, the chopping box 23 is internally provided with a chopping bin 26, the chopping bin 26 is internally provided with a third hydraulic machine 24, the lower end of the third hydraulic machine 24 is slidably connected with a second hydraulic rod 25, the lower end of the second hydraulic rod 25 is fixedly connected with a chopping blade 27, the chopping blade 27 is slidably connected with the inner wall of the chopping bin 26, a sampling bottle 42 is arranged below the chopping blade 27, the sampling bottle 42 is slidably connected with a clamping plate 41, the clamping plate 41 is fixedly connected with a fixed block 40, the sampling bottle 42 is internally provided with a sample bin 112, the sample bin 112 is internally provided with a third pressure sensor 43, and the third pressure sensor 43 is fixedly connected with the inner wall of the sample bin 112, the upper end face of the third pressure sensor 43 is fixedly connected with a sampling spring 44, the upper end of the sampling spring 44 is fixedly connected to a pressure plate 45, the pressure plate 45 is slidably connected with the inner wall of the sample bin 112, the fixed block 40 is fixedly connected to the conveying belt 38, the chopping blade 27 descends to chop the sampling cotton swab, the sampling cotton swab falls into the sampling bottle 42, and the sampling cotton swab is conveyed to a next process under the action of the conveying belt 38.
Advantageously, the stable clamping mechanism 201 further comprises a rack 105, the rack 105 is fixedly connected to the right end face of the lifting rod 101, the rack 105 is engaged with the driving gear 106, the driving gear 106 is fixedly connected to the front end of the driving rotating shaft 107, the driving rotating shaft 107 is rotatably connected with the third motor 108, and the third motor 108 is fixedly connected to the inner wall of the rear side of the empty bin 12;
advantageously, the swab sampling mechanism 202 further comprises a feeding box 21, the feeding box 21 is fixedly connected to the upper end wall of the box body 11, a feeding bin 22 is arranged in the feeding box 21, the feeding bin 22 faces the blanking shaft 67, the electronic eye 91 is fixedly connected to the upper end of the swab fixing pipe 83, the display screen 20 is fixedly connected to the upper end of the box body 11, two ultraviolet sterilizers 13 are fixedly connected to the upper inner wall of the empty bin 12, the two ultraviolet sterilizers 13 are symmetrical with respect to the center line of the empty bin 12, a second motor 53 is further arranged in the sampling plate 52, the second motor 53 is fixedly connected in the sampling plate 52, a second driving shaft 54 is rotatably connected to the left end face of the second motor 53, a first bevel gear 55 is fixedly connected to the left end of the second driving shaft 54, and the first bevel gear 55 can be engaged with a second bevel gear 56, an iron movable plate 60 is rotatably connected to the lower end of the second bevel gear 56, the iron movable plate 60 is slidably connected with a movable bin 57, the movable bin 57 is arranged in the sampling plate 52, a movable spring 59 is fixedly connected to the lower end of the iron movable plate 60, the lower end of the movable spring 59 is fixedly connected to a first electromagnet 58, the first electromagnet 58 is fixedly connected to the inner wall of the lower side of the movable bin 57, the second bevel gear 56 can also be meshed with a third bevel gear 61, and the left end face of the third bevel gear 61 is fixedly connected with the rotating shaft 62;
advantageously, the sample bottling mechanism 203 further comprises a first motor 36, the first motor 36 is fixedly connected to the rear end surface of the box 11, the right end surface of the first motor 36 is rotatably connected to a first driving shaft 37, a transmission wheel 111 is fixedly connected to the first driving shaft 37, the transmission wheel 111 is in friction transmission with the transmission wheel 111 on the front side through the transmission belt 38, a limiting rod 99 is further fixedly connected to the rear end surface of the box 11, a limiting block 98 is fixedly connected to the rear end of the limiting rod 99, a baffle 100 is further fixedly connected to the transmission belt 38, the chopping blade 27 is embedded with a first inductor 28, the first inductor 28 can mutually sense with a second embedded inductor 92, and the second inductor 92 is located in the swab fixing tube 83.
In the initial state, the sampling spring 44, the movable spring 59, the slide spring 77, the lift spring 104, the clamp spring 34 are in the compressed state, the piston spring 90 is in the normal state, the support spring 96, and the drive spring 81 are in the extended state.
The required sampling cotton swab is ready to be put into the feeding bin 22 in a specific direction, the cotton swab under the action of gravity falls onto the blanking shaft 67, the second motor 53 operates to drive the second driving shaft 54 to rotate, so the first bevel gear 55 starts to rotate, the first bevel gear 55 drives the second bevel gear 56 engaged with the first bevel gear 55 to rotate, the second bevel gear 56 drives the third bevel gear 61 engaged with the second bevel gear to rotate, the third bevel gear 61 drives the rotating shaft 62 to rotate, the rotating shaft 62 drives the blanking shaft 67 to rotate, the cotton swab clamped into the blanking shaft 67 rotates along with the blanking shaft 67 until the cotton swab falls into the pushing bin 64 under the action of gravity, the cotton swab presses the sliding plate 78, the sliding plate 78 descends to compress the sliding spring 77, so after the second pressure sensor 76 senses the pressure, the first electromagnet 58 electrically operates to give an attractive force to the iron movable plate 60 to descend the iron movable plate 60, the movable spring 59 is compressed, the second bevel gear 56 is disengaged from the first bevel gear 55 and the third bevel gear 61, so that the blanking shaft 67 does not rotate any more, the descending sliding plate 78 drives the supporting rod 74 and the conductive iron block 73 to descend, the conductive iron block 73 descends to be in contact with the conductive block 71, the circuit is closed, so that the second annunciator 72 works to enable the fifth electromagnet 66 to work by electrifying to give a repulsive force to the pushing plate 63, the pushing spring 65 is stretched, the pushing plate 63 moves leftwards to push the cotton swab to enter the pipeline 84, the first annunciator 70 works to enable the third electromagnet 95 to work to give a repulsive force to the magnetic supporting block 97, the magnetic supporting block 97 moves downwards to clamp the stretched cotton swab, and the sampling cotton swab is ready;
informing the person to be detected to place the chin on the clamping soft body 35, lowering the clamping soft body 35 to compress the clamping spring 34 after the clamping soft body 35 is subjected to pressure, after the first pressure sensor 33 senses the pressure by the compression of the clamping spring 34, starting the third motor 108 to work to drive the driving rotating shaft 107 to rotate, driving the driving rotating shaft 107 to drive the driving gear 106 to rotate, driving the driving gear 106 to move the rack 105 meshed with the driving gear, driving the lifting rod 101 to move by the movement of the rack 105, driving the stabilizing plate 29 fixedly connected with the lifting rod 101 to move downwards, so that the clamping plate 30 moves downwards to abut against the head of the person to be detected, keeping the person to be detected stable and motionless, controlling the operation of the second hydraulic machine 47 by the operator, moving the third hydraulic rod 48 up and down by the second hydraulic machine 47, moving the hydraulic plate 49 up and down by the third hydraulic rod 48, moving the hydraulic plate 49 driving the hydraulic plate 50 to move up and down, the moving plate 50 moves up and down to adjust the height of the cotton swab clamped in the pipeline 84 to enable the cotton swab to be suitable for a person to be detected, then the operator checks the display screen 20 to operate, the display screen 20 is connected with the electronic eye 91, the operator operates the first hydraulic press 17, the first hydraulic press 17 pushes the first hydraulic rod 18 to move left, the first hydraulic rod 18 moves left to enable the cotton swab to move left and slowly enter the mouth of the person to be detected, the instrument is adjusted in real time by observing the display screen 20, when the cotton swab reaches a preset position, the fourth electromagnet 79 is electrified to work to give a repulsive force to the driving block 82, the driving block 82 pushes the cotton swab fixing pipe 83 downwards, the cotton swab fixing pipe 83 rotates, the cotton swab rotates to rub the upper jaw of the person to be detected to sample, after the cotton swab is completely sampled, the first hydraulic press 17 is controlled to withdraw the cotton swab into the empty bin 12, and the third hydraulic press 24 works to push the second hydraulic press 25 to descend after the cotton swab returns to the original position, after the chopping blade 27 descends, the first sensor 28 senses the second sensor 92, the chopping blade 27 cuts off the cotton swabs, the chopping blade 27 stops descending to return to the original position, the cotton swabs in the sampling part fall to the sample bin 112 to compress the pressure plate 45, the pressure plate 45 descends the sampling spring 44 to compress the third pressure sensor 43 to sense the pressure, the first motor 36 works to drive the first driving shaft 37 to enable the conveying belt 38 to start moving, the spring fixedly connected with the fixed block 40 is stretched, the sampling bottle 42 moved out of the empty bin 12 is taken out for next detection, a new sampling bottle 42 is replaced, the first motor 36 stops working, and the fixed block 40 returns to the original position under the action of the spring fixedly connected with the fixed block 40;
the second electromagnet 89 is electrified to give an attractive force to the piston plates 85, the two piston plates 85 are separated, the residual sticks fall, the sliding plate 78 losing pressure rises, the first electromagnet 58 stops being electrified, the iron movable plate 60 and the second bevel gear 56 rise, the rising second bevel gear 56 is meshed with the third bevel gear 61 and the first bevel gear 55, the blanking shaft 67 starts rotating, and the next cotton swab falls into the pushing bin 64 to wait for the next person to be detected.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (4)
1. The utility model provides a mycoplasma cell detecting instrument based on pcr technique, includes the box, its characterized in that: the box body is internally provided with an empty bin, the empty bin is internally provided with a stable clamping mechanism, the stable clamping mechanism comprises a stable plate, the stable plate is fixedly connected with two clamping blocks, the two clamping blocks are symmetrical front and back by taking the center line of the stable plate as the center, each clamping block is in sliding connection with a sliding chute, the sliding chute is arranged in the box body, the lower end surface of the stable plate is fixedly connected with a clamping plate, the lower end surface of the stable plate is fixedly connected with a lifting rod, the lifting rod is in sliding connection with a lifting bin, the lifting bin is arranged in a lifting box, the lifting box is fixedly connected with the lower inner wall of the empty bin, the lifting bin is also internally provided with a lifting spring, the lower end of the lifting spring is fixedly connected onto the lower inner wall of the lifting bin, the upper end of the lifting spring is fixedly connected onto the lifting rod, and the left end surface of the box body is fixedly connected with a fixed plate, an induction bin with an upward opening is arranged in the fixing plate, a first pressure inductor is arranged in the induction bin, a clamping spring is fixedly connected to the upper end face of the first pressure inductor, a clamping soft body is fixedly connected to the upper end of the clamping spring, the clamping soft body is in sliding connection with the inner wall of the induction bin, a face of a person to be sampled is placed on the fixing plate, and the clamping plate stably clamps the face along with the descending of the stabilizing plate to wait for the next operation;
a cotton swab sampling mechanism is further arranged in the empty bin and comprises a movable plate, the movable plate is fixedly connected to the inner wall of the lower side of the empty bin, a movable groove is formed in the movable plate and is connected with a movable clamping block in a sliding mode, the movable clamping block is fixedly connected to a first hydraulic machine, the first hydraulic machine is connected with the left end face of the movable plate in a sliding mode, the left end face of the first hydraulic machine is connected with a first hydraulic rod in an interactive mode, the left end of the first hydraulic rod is fixedly connected to the sampling plate, the inner wall of the lower side of the empty bin is fixedly connected with a second hydraulic machine, the upper end face of the second hydraulic machine is connected with a third hydraulic rod in a sliding mode, the third hydraulic rod is connected with a guide plate in a sliding mode, the guide plate is fixedly connected to the inner wall of the lower side of the empty bin, and the upper end of the third hydraulic rod is fixedly connected to the hydraulic plate, the hydraulic plate is fixedly connected to the lower end of a movable plate, two movable clamping blocks are arranged on the movable plate and are connected with the movable plate in a sliding mode, the movable clamping blocks are fixedly connected to the lower end face of the sampling plate, a rotating shaft is connected to the sampling plate in a rotating mode, a blanking shaft is fixedly connected to the rotating shaft, a pushing bin is arranged below the blanking shaft, a fifth electromagnet is arranged in the pushing bin and is fixedly connected to the inner wall of the right side of the pushing bin, a pushing spring is fixedly connected to the left end of the fifth electromagnet, the left end of the pushing spring is fixedly connected to the pushing plate, the pushing plate is connected with the inner wall of the pushing bin in a sliding mode, a sliding plate is further arranged in the pushing bin and is connected with the sliding bin in a sliding mode, the sliding bin is arranged in the sampling plate, and a second pressure sensor is further arranged in the sliding bin, the second pressure sensor is fixedly connected to the lower inner wall of the sliding bin, the upper end of the second pressure sensor is fixedly connected with a sliding spring, the upper end of the sliding spring is fixedly connected with the sliding plate, the sliding plate is fixedly connected with a supporting rod, the right end of the supporting rod is fixedly connected with a conductive iron block, the conductive iron block can be in contact with two conductive blocks, the two conductive blocks are symmetrical front and back by taking the center line of the conductive iron block as the center, the conductive blocks are fixedly connected to the lower inner wall of the circuit bin, the circuit bin is arranged in the sampling plate, the conductive block at the rear side is connected with a first annunciator through an electric wire, the first annunciator is fixedly connected to the lower inner wall of the circuit bin, the first annunciator is connected with a power supply through an electric wire, the power supply is fixedly connected to the lower inner wall of the circuit bin, and the power supply is connected with the second annunciator through an electric wire, the second annunciator is fixedly connected to the lower inner wall of the circuit bin, the second annunciator is connected with the conductive block on the front side through an electric wire, the sampling plate is internally provided with a driving bin, the driving bin is internally provided with a fourth electromagnet, the fourth electromagnet is fixedly connected to the upper inner wall of the driving bin, the lower end face of the fourth electromagnet is fixedly connected with a driving spring, the lower end of the driving spring is fixedly connected with a driving block, the driving block is in sliding connection with the inner wall of the driving bin, the lower end of the driving block extends into a rotating bin, the rotating bin is arranged in the sampling plate, the lower end of the driving block is in contact with a cotton swab fixing pipe, the cotton swab fixing pipe is fixedly connected with two rotating shafts, the two rotating shafts are symmetrical around the central line of the driving block, one end of the rotating shaft, which is far away from the driving block, is rotatably connected with the inner walls on the front side and the rear side of the rotating bin, a piston bin is arranged in the cotton swab fixing pipe, a second electromagnet is arranged in the piston bin, the second electromagnet is fixedly connected in the piston bin, a piston spring is fixedly connected to the front end surface and the rear end surface of the second electromagnet respectively, the two piston springs are symmetrical front and back by taking the center line of the second electromagnet as the center, the other end of each piston spring is fixedly connected with a piston plate, the piston plates are connected with the inner wall of the piston bin in a sliding manner, the two piston plates are mutually contacted, a pipeline is arranged in the cotton swab fixing pipe, a magnetic supporting block is arranged in the pipeline and slides with the inner wall of the supporting bin, the supporting bin is arranged in a supporting bin, the supporting bin is fixedly connected with the cotton swab fixing pipe, a third electromagnet is further arranged in the supporting bin and is fixedly connected to the inner wall of the upper side of the supporting bin, the lower end face of the third electromagnet is fixedly connected with a supporting spring, the other end of the supporting spring is fixedly connected with the magnetic supporting block, the height of the cotton swab fixing pipe is adjusted, a sampling cotton swab falls from the blanking shaft into the pushing bin and is pushed into the pipeline by the pushing plate to be clamped by the magnetic supporting block, and the cotton swab extends into the mouth of a person to be sampled along with the cotton swab fixing pipe under the control of an operator for sampling;
the sample bottling mechanism is also arranged in the empty bin and comprises a chopping box, the chopping box is fixedly connected to the inner wall of the upper side of the empty bin, the chopping bin is arranged in the chopping box, a third hydraulic machine is arranged in the chopping bin, the lower end of the third hydraulic machine is connected with a second hydraulic rod in a sliding manner, the lower end of the second hydraulic rod is fixedly connected with a chopping blade, the chopping blade is connected with the inner wall of the chopping bin in a sliding manner, a sampling bottle is arranged below the chopping blade and is connected with a clamping plate in a sliding manner, the clamping plate is fixedly connected to a fixed block, a sample bin is arranged in the sampling bottle, a third pressure sensor is arranged in the sample bin and is fixedly connected with the inner wall of the sample bin, a sampling spring is fixedly connected to the upper end face of the third pressure sensor, and the upper end of the sampling spring is fixedly connected to a pressure plate, the pressure plate is connected with the inner wall of the sample bin in a sliding mode, the fixing block is fixedly connected to the conveying belt, the chopping blade descends to chop the sampling cotton swabs, the sampling cotton swabs fall into the sampling bottles, and the sampling cotton swabs are conveyed to the next process to be processed under the action of the conveying belt.
2. The pcr-based mycoplasma cell detection apparatus according to claim 1, wherein: stabilize fixture still includes the rack, rack fixed connection be in on the right-hand member face of lifter, rack and drive gear meshing, drive gear fixed connection is at the front end of drive pivot, the drive pivot rotates with the third motor to be connected, third motor fixed connection be in on the rear side inner wall of empty storehouse.
3. The pcr-based mycoplasma cell detection apparatus according to claim 1, wherein: the cotton swab sampling mechanism further comprises a feeding box, the feeding box is fixedly connected to the upper end wall of the box body, a feeding bin is arranged in the feeding box and faces the blanking shaft, an electronic eye is fixedly connected to the upper end of the cotton swab fixing pipe, a display screen is fixedly connected to the upper end of the box body, two ultraviolet disinfectors are fixedly connected to the inner wall of the upper side of the empty bin, the two ultraviolet disinfectors are bilaterally symmetrical with the center line of the empty bin as the center, a second motor is further arranged in the sampling plate and fixedly connected to the sampling plate, a second driving shaft is rotatably connected to the left end face of the second motor, a first bevel gear is fixedly connected to the left end of the second driving shaft and can be meshed with a second bevel gear, and an iron movable plate is rotatably connected to the lower end of the second bevel gear, the iron fly leaf and activity storehouse sliding connection, the activity storehouse is established in the sampling plate, the lower extreme fixedly connected with movable spring of iron fly leaf, on the first electro-magnet of lower extreme fixed connection of movable spring, first electro-magnet with the downside inner wall fixed connection in activity storehouse, second bevel gear also can mesh with third bevel gear, third bevel gear's left end face fixedly connected with the axis of rotation.
4. The pcr-based mycoplasma cell detection apparatus according to claim 1, wherein: the sample bottling mechanism further comprises a first motor, the first motor is fixedly connected to the rear end face of the box body, a first driving shaft is rotatably connected to the right end face of the first motor, a conveying wheel is fixedly connected to the first driving shaft, the conveying wheel is in friction transmission with the conveying wheel on the front side through the conveying belt, a limiting rod is further fixedly connected to the rear end face of the box body, a limiting block is fixedly connected to the rear end of the limiting rod, a baffle is further fixedly connected to the conveying belt, a first inductor is embedded in the chopping blade, the first inductor can mutually sense with a second inductor, and the second inductor is embedded in the cotton swab fixing pipe.
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| CN202011115224.9A CN112226346B (en) | 2020-10-19 | 2020-10-19 | Cell mycoplasma detecting instrument based on pcr technology |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201812017A (en) * | 2016-09-12 | 2018-04-01 | 長春藤生命科學股份有限公司 | Method for rapidly detecting the content of bacteria and mycoplasma in cell samples allowing simultaneous and rapid detection to find bacteria and mycoplasma and effectively reducing the cost |
| CN110074825A (en) * | 2019-05-15 | 2019-08-02 | 义乌奥莉机械科技有限公司 | A kind of tyre oral cavity material sampling instrument |
| CN110618283A (en) * | 2019-11-12 | 2019-12-27 | 淄博海瑞医疗器械有限公司 | Automatic change urine and diagnose detection device |
| CN111595759A (en) * | 2020-06-04 | 2020-08-28 | 李五世 | Cell detection device with private storehouse of subregion sampling |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100615424B1 (en) * | 2004-01-14 | 2006-08-25 | 김철민 | Oligonucleotide for genotyping of Mycoplasma, microarray comprising the oligonucleotide, and method for detection of species using the microarray |
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2020
- 2020-10-19 CN CN202011115224.9A patent/CN112226346B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201812017A (en) * | 2016-09-12 | 2018-04-01 | 長春藤生命科學股份有限公司 | Method for rapidly detecting the content of bacteria and mycoplasma in cell samples allowing simultaneous and rapid detection to find bacteria and mycoplasma and effectively reducing the cost |
| CN110074825A (en) * | 2019-05-15 | 2019-08-02 | 义乌奥莉机械科技有限公司 | A kind of tyre oral cavity material sampling instrument |
| CN110618283A (en) * | 2019-11-12 | 2019-12-27 | 淄博海瑞医疗器械有限公司 | Automatic change urine and diagnose detection device |
| CN111595759A (en) * | 2020-06-04 | 2020-08-28 | 李五世 | Cell detection device with private storehouse of subregion sampling |
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