CN112220892A

CN112220892A - Plant nitric oxide supply and preparation method and application thereof

Info

Publication number: CN112220892A
Application number: CN202011173402.3A
Authority: CN
Inventors: 张妍; 郭长江; 罗红梅
Original assignee: Zhengzhou En Eu Biotechnology Co ltd
Current assignee: Zhengzhou En Eu Biotechnology Co ltd
Priority date: 2020-10-28
Filing date: 2020-10-28
Publication date: 2021-01-15

Abstract

The invention relates to a plant nitric oxide donor and a preparation method and application thereof, wherein the formula raw materials of the nitric oxide donor comprise, by mass, 10-20 parts of black fungus extract, 10-20 parts of mushroom extract, 20-30 parts of honey extract, 10-20 parts of Chinese date extract, 10-20 parts of eggplant extract, 10-20 parts of tomato extract, 10-20 parts of sweet potato extract, 10-20 parts of corn extract and 10-20 parts of onion extract. The invention adopts the freeze-drying method and the tape casting film forming method to prepare fibrous, film-shaped or porous materials, is used for preparing artificial blood vessels, vascular stents or wound dressings, has the characteristics of resisting platelet adhesion, promoting the growth of vascular endothelial cells and inhibiting the growth of smooth muscle cells, and can be used for the treatment of cardiovascular diseases and the effect of skin repair.

Description

Plant nitric oxide supply and preparation method and application thereof

Technical Field

The invention relates to the technical field of nitric oxide donors, in particular to a plant nitric oxide donor, and a preparation method and application thereof.

Background

The key to the project is the application of natural nitric oxide donor transformation in vivo to supplement nitric oxide to prevent and treat cardiovascular diseases including hypertension, the nitric oxide exists widely in vivo, but due to short half-life (only a few milliseconds) and extreme instability, continuous supplement is needed to meet the normal physiological needs of the body and maintain health, and the nitric oxide is sourced from endogenous synthesis and exogenous supplement.

Endogenous synthesis takes arginine, oxygen and NADPH as substrates, and nitric oxide and citrulline are catalytically generated by Nitric Oxide Synthase (NOS). Although a healthy human body has sufficient nitric oxide synthase to produce nitric oxide, the total activity of nitric oxide synthase in the human body is reduced with age due to various factors such as the reduction of gene expression in cell aging, and the production of nitric oxide in the human body is also reduced accordingly. For example, by the age of forty, nitric oxide is generated in healthy people, which is half or less than that of twenty years old, and causes dysfunction of vascular endothelial cells, and white blood cells and platelets are gathered on the vascular wall to cause atherosclerosis of the vascular wall. This may also explain why blood pressure rises with age. More importantly, the modern life has many factors to influence the nitric oxide, such as chronic diseases, smoking, air pollution, unhealthy diet, bad living habits, over-pressure and the like, which can directly or indirectly cause the reduction of the nitric oxide in the body.

Exogenous supplementation can be converted to nitric oxide in vivo by ingestion of food and drug nitric oxide donors. Clinically, commonly used nitrate-based drugs for treating angina pectoris and heart failure, such as nitroglycerin and Xiaocardia pain, expand blood vessels by supplying exogenous nitric oxide to vascular smooth muscle, relieve coronary spasm, increase blood volume in ischemic region, and reduce oxygen consumption of myocardium, so as to relieve symptoms of angina pectoris and heart failure. Nitrate drugs such as glycerol trinitrate can only temporarily relieve angina pectoris symptoms of coronary heart disease, cannot delay the progress of the disease course, and have no improvement effect on prognosis. Meanwhile, the medicine for supplementing nitric oxide is usually only used for emergency due to large dosage, quick response and short duration

The nitric oxide donors are quick to absorb (1-2 minutes of action), short in drug effect (the half-life period is only 7 minutes), and capable of effectively relieving cardiovascular pressure, but because nitric oxide is quickly released in a large amount to cause vascular irritation and relaxation, the common clinical side effects such as headache, palpitation, hypotension, syncope and the like are caused, drug resistance is generated after long-term large-amount administration, and therefore, the drug supplementing nitric oxide cannot be used as a conventional nitric oxide supplementing way.

Disclosure of Invention

The invention aims to provide a plant nitric oxide supply and a preparation method and application thereof.

The above object of the present invention is achieved by the following technical solutions:

the plant nitric oxide donor is characterized in that the formula raw materials of the nitric oxide donor comprise, by mass, 10-20 parts of black fungus extract, 10-20 parts of mushroom extract, 20-30 parts of honey extract, 10-20 parts of Chinese date extract, 10-20 parts of eggplant extract, 10-20 parts of tomato extract, 10-20 parts of sweet potato extract, 10-20 parts of corn extract and 10-20 parts of onion extract.

Preferably, the nitric oxide donor formula raw materials comprise, by mass, 10 parts of black fungus extract, 10 parts of mushroom extract, 20 parts of honey extract, 10 parts of Chinese date extract, 10 parts of eggplant extract, 10 parts of tomato extract, 10 parts of sweet potato extract, 10 parts of corn extract and 10 parts of onion extract.

Preferably, the nitric oxide donor formula raw materials comprise, by mass, 20 parts of black fungus extract, 20 parts of mushroom extract, 30 parts of honey extract, 20 parts of Chinese date extract, 20 parts of eggplant extract, 20 parts of tomato extract, 20 parts of sweet potato extract, 20 parts of corn extract and 20 parts of onion extract.

Preferably, the nitric oxide donor formula raw materials comprise, by mass, 15 parts of black fungus extract, 15 parts of mushroom extract, 25 parts of honey extract, 15 parts of Chinese date extract, 15 parts of eggplant extract, 15 parts of tomato extract, 15 parts of sweet potato extract, 15 parts of corn extract and 15 parts of onion extract.

A preparation method of a plant nitric oxide donor comprises the following preparation steps:

step 1: putting black fungus, mushroom, Chinese date, eggplant, tomato, sweet potato, corn and onion into a grinding tank for grinding treatment, and grinding into powder;

step 2: putting the powder obtained in the step 1 into a centrifuge, adding honey and the extract, centrifuging by a relative centrifugal force of below 5000g for 10-15 min, taking out the upper-layer mixed solution, centrifuging for the second time, repeating the step for 5 times, and collecting the upper-layer mixed solution;

and step 3: and (3) carrying out spray drying on the mixed solution finally obtained in the step (2) to obtain the nitric oxide donor.

The application of the plant nitric oxide donor in the wound healing dressing is to blend and compound the plant nitric oxide donor and a polymer, prepare a fibrous, membranous or porous material by adopting a freeze drying method and a tape casting film forming method, and use the fibrous, membranous or porous material in the preparation of artificial blood vessels, vascular stents or wound dressings.

In conclusion, the beneficial technical effects of the invention are as follows:

1. the nitric oxide donor can be applied to preparing artificial blood vessels or blood vessel stents, wound dressings and the like. The plant nitric oxide donor and the polymer can be blended and compounded, and prepared into fibrous, film-shaped or porous materials by adopting an electrostatic spinning method, a freeze-drying method, a casting film forming method and the like, and the materials are used for artificial blood vessels, vascular stents, wound dressings and the like, for example, the nitric oxide donor and the polymer can be blended and compounded to prepare films or coatings; or blending with other polymers, casting to form a film, and particularly, can be used for preparing materials such as artificial blood vessels, vascular stents, wound dressings and the like;

2. can be easily compounded with other materials to prepare biomedical materials, has the characteristics of resisting platelet adhesion, promoting the growth of endothelial cells in blood vessels and inhibiting the growth of smooth muscle cells, and can be used for treating cardiovascular diseases, repairing skin and the like.

Detailed Description

The present invention will be described in further detail with reference to examples.

Example 1

The invention discloses a plant nitric oxide donor, which comprises the following raw materials, by mass, 10 parts of black fungus extract, 10 parts of mushroom extract, 20 parts of honey extract, 10 parts of Chinese date extract, 10 parts of eggplant extract, 10 parts of tomato extract, 10 parts of sweet potato extract, 10 parts of corn extract and 10 parts of onion extract.

Example 2

The invention discloses a plant nitric oxide donor, which comprises the following raw materials, by mass, 20 parts of black fungus extract, 20 parts of mushroom extract, 30 parts of honey extract, 20 parts of Chinese date extract, 20 parts of eggplant extract, 20 parts of tomato extract, 20 parts of sweet potato extract, 20 parts of corn extract and 20 parts of onion extract.

Example 3

The invention discloses a plant nitric oxide donor, which comprises the following raw materials, by mass, 15 parts of black fungus extract, 15 parts of mushroom extract, 25 parts of honey extract, 15 parts of Chinese date extract, 15 parts of eggplant extract, 15 parts of tomato extract, 15 parts of sweet potato extract, 15 parts of corn extract and 15 parts of onion extract.

The application of the plant nitric oxide donor in the wound healing dressing is to blend and compound the plant nitric oxide donor and a polymer, prepare a fibrous, membranous or porous material by adopting a freeze drying method and a tape casting film forming method, and use the fibrous, membranous or porous material in the preparation of artificial blood vessels, vascular stents or wound dressings. .

The implementation principle of the embodiment is as follows: the nitric oxide donor can be applied to preparing artificial blood vessels or blood vessel stents, wound dressings and the like. The plant nitric oxide donor and the polymer can be blended and compounded, and prepared into fibrous, film-shaped or porous materials by adopting an electrostatic spinning method, a freeze-drying method, a casting film forming method and the like, and the materials are used for artificial blood vessels, vascular stents, wound dressings and the like, for example, the nitric oxide donor and the polymer can be blended and compounded to prepare films or coatings; or blending with other polymers, casting to form a film, and particularly, can be used for preparing materials such as artificial blood vessels, vascular stents, wound dressings and the like; can be easily compounded with other materials to prepare biomedical materials, has the characteristics of resisting platelet adhesion, promoting the growth of endothelial cells in blood vessels and inhibiting the growth of smooth muscle cells, and can be used for treating cardiovascular diseases, repairing skin and the like.

The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.

Claims

1. The plant nitric oxide donor is characterized in that the formula raw materials of the nitric oxide donor comprise, by mass, 10-20 parts of black fungus extract, 10-20 parts of mushroom extract, 20-30 parts of honey extract, 10-20 parts of Chinese date extract, 10-20 parts of eggplant extract, 10-20 parts of tomato extract, 10-20 parts of sweet potato extract, 10-20 parts of corn extract and 10-20 parts of onion extract.

2. The plant nitric oxide donor according to claim 1, wherein the nitric oxide donor formula comprises, by mass, 10 parts of black fungus extract, 10 parts of mushroom extract, 20 parts of honey extract, 10 parts of jujube extract, 10 parts of eggplant extract, 10 parts of tomato extract, 10 parts of sweet potato extract, 10 parts of corn extract and 10 parts of onion extract.

3. The plant nitric oxide donor according to claim 1, wherein the nitric oxide donor formula comprises, by mass, 20 parts of black fungus extract, 20 parts of mushroom extract, 30 parts of honey extract, 20 parts of jujube extract, 20 parts of eggplant extract, 20 parts of tomato extract, 20 parts of sweet potato extract, 20 parts of corn extract and 20 parts of onion extract.

4. The plant nitric oxide donor according to claim 1, wherein the nitric oxide donor formula comprises, by mass, 15 parts of black fungus extract, 15 parts of mushroom extract, 25 parts of honey extract, 15 parts of jujube extract, 15 parts of eggplant extract, 15 parts of tomato extract, 15 parts of sweet potato extract, 15 parts of corn extract and 15 parts of onion extract.

5. The method of claim 1, comprising the steps of:

6. The application of the plant nitric oxide donor in the wound healing dressing according to claim 1, wherein the plant nitric oxide donor and the polymer are blended and compounded, and the mixture is prepared into a fibrous, film-shaped or porous material by adopting a freeze drying method and a casting film forming method, and is used for preparing an artificial blood vessel, a blood vessel stent or a wound dressing.