CN112213485A - Magnetic nano-microsphere as p-hydroxy phenylalanine urine detection reagent and preparation method thereof - Google Patents
Magnetic nano-microsphere as p-hydroxy phenylalanine urine detection reagent and preparation method thereof Download PDFInfo
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6806—Determination of free amino acids
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Abstract
The invention discloses a magnetic nano microsphere of a p-hydroxy-phenylalanine urine detection reagent and a preparation method thereof, wherein the magnetic nano microsphere comprises 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of a surfactant; according to the preparation method, each reagent is independently wrapped by the microsphere polymer shell substrate, so that the stability of the detection reagent can be improved, a complex preparation process is not needed, the detection can be carried out after acid liquor is mixed, the portable detection equipment is convenient to manufacture, and the accuracy of urine detection is improved.
Description
Technical Field
The invention relates to the field of detection reagents, in particular to a magnetic nano microsphere for a p-hydroxy phenylalanine urine detection reagent and a preparation method thereof.
Background
The p-hydroxy phenylalanine urine detection reagent is mainly used for qualitatively detecting the content of p-hydroxy phenylalanine in urine so as to judge the metabolic activity degree of malignant tumor cells in a human body and further carry out malignant tumor risk assessment. The malignant tumor patients generally have the phenomenon of abnormal amino acid metabolism, namely, the process of decomposing protein into amino acid is enhanced, the catabolism of the amino acid is weakened, the amino acid can be reused for synthesizing tumor cell protein, and the redundant amino acid which cannot be utilized is discharged out of the body. Amino acid metabolism abnormalities exist early in tumorigenesis, and one important manifestation is that the urine contains elevated levels of p-hydroxyphenylalanine (tyrosine). The more active the tumor cell metabolism, the more remarkable the amino acid metabolism abnormality and the more remarkable the tyrosine content increase. Through a large number of experimental researches, the tyrosine content in the urine of almost all malignant tumor patients is obviously increased by 50-150%. Therefore, the p-hydroxyphenylalanine urine detection reagent is mainly applied to discovery of primary tumors, screening of high risk groups of tumors, observation and evaluation of tumor treatment effects, prediction of tumor recurrence and prognosis and the like clinically.
The operation is complex and can be completed only by a laboratory with complete appliances, if the operation is carried out in advance, the operation is carried out to prepare test paper, the stability is low, the urine test accuracy is not high, the storage time of a detection reagent is longer, the color change is not obvious, the accuracy is lower, the portability is not high, the operation must be carried out in the laboratory, the convenience is low, and the detection quality is reduced; the market needs a method for enhancing the stability of the detection reagent, improving the storage time limit of the detection reagent and facilitating the preparation of portable detection equipment; the present invention solves such problems.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide the magnetic nano-microsphere for the p-hydroxyphenylalanine urine detection reagent and the preparation method thereof, which can improve the stability of the detection reagent, do not need a complex preparation process, can be used after being mixed with acid liquor for detection, are convenient to be made into portable detection equipment, and improve the accuracy of urine detection.
In order to achieve the above object, the present invention adopts the following technical solutions:
a magnetic nano microsphere of a p-hydroxyphenylalanine urine detection reagent comprises: the formula comprises the following components:
26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant.
In the magnetic nano microsphere of the p-hydroxy phenylalanine urine detection reagent, the acid-soluble chitosan is terephthalaldehyde crosslinked chitosan.
The magnetic nanoparticle of the p-hydroxyphenylalanine urine detection reagent comprises: iron oxide nanoparticles, superparamagnetic iron oxide nanoparticles.
In the magnetic nano microsphere as the p-hydroxyphenylalanine urine detection reagent, the surfactant is sodium secondary alkyl sulfate with the concentration of 1%.
A preparation method of a p-hydroxyphenylalanine urine detection reagent magnetic nano-microsphere comprises the following steps:
preparing materials according to a formula;
the formula comprises the following components: 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant;
preparing mercury sulfate magnetic nano microspheres:
step 1, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step 2, microsphere polymer core layer solution:
mixing mercury sulfate with 24-46 parts of surfactant;
step 3, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercury sulfate magnetic nano microspheres;
preparing mercurous nitrate magnetic nano microspheres:
step a, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step b, preparing a microsphere polymer core layer solution:
mercurous nitrate and 32-54 parts of surfactant;
step c, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercurous nitrate magnetic nano microspheres;
preparing nickel sulfate magnetic nano microspheres:
in the step A, the step B is carried out,
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step B, microsphere polymer core layer solution:
mixing nickel sulfate and 4-12 parts of surfactant;
step C, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain nickel sulfate magnetic nano microspheres;
preparing alpha-nitroso-beta-naphthol magnetic nano microspheres:
step I, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step II, preparing a microsphere polymer core layer solution:
mixing alpha-nitroso-beta-naphthol and 4-8 parts of surfactant;
step III, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain the alpha-nitroso-beta-naphthol magnetic nano-microsphere;
mixing the mercury sulfate magnetic nano-microspheres, the mercurous nitrate magnetic nano-microspheres, the nickel sulfate magnetic nano-microspheres and the alpha-nitroso-beta-naphthol magnetic nano-microspheres according to the volume ratio of 2-3:2.2-4.4:1.6-2.2:0.8-1.2 to obtain the para-hydroxy phenylalanine urine detection reagent magnetic nano-microspheres.
In the preparation method of the magnetic nano-microsphere of the p-hydroxyphenylalanine urine detection reagent,
the specific preparation method of the microsphere polymer shell substrate comprises the following steps: dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate.
According to the preparation method of the magnetic nano-microsphere of the p-hydroxy-phenylalanine urine detection reagent, the magnetic nano-microsphere of mercury sulfate, the magnetic nano-microsphere of mercurous nitrate, the magnetic nano-microsphere of nickel sulfate and the magnetic nano-microsphere of alpha-nitroso-beta-naphthol are mixed according to the volume ratio of 2.2:3.6:1.8:1.0 to obtain the magnetic nano-microsphere of the p-hydroxy-phenylalanine urine detection reagent.
In the preparation method of the magnetic nano microsphere as the p-hydroxyphenylalanine urine detection reagent, the method for wrapping the microsphere polymer core layer solution by the microsphere polymer shell layer substrate by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; and after the injection is finished, the nano microspheres are obtained by finishing the curing in a curing bath.
The invention has the advantages that:
according to the invention, each reagent is independently wrapped by the microsphere polymer shell substrate, so that the stability of the detection reagent can be improved, a complex preparation process is not required, the detection can be carried out after acid liquor is mixed, the portable detection equipment is convenient to manufacture, and the accuracy of urine detection is improved;
the formula adopted by the microsphere polymer shell substrate is matched with the magnetic nanoparticles, so that microspheres carrying various reagents can be rapidly aggregated together when in use, the dissolving speed is increased, and the detection speed is increased.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
A magnetic nano microsphere of a p-hydroxyphenylalanine urine detection reagent comprises: the formula comprises the following components:
26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant. As an example, the acid-soluble chitosan is terephthalaldehyde crosslinked chitosan, and the surfactant is sodium secondary alkyl sulfate at a concentration of 1%. As an example, the magnetic nanoparticles include: iron oxide nanoparticles, superparamagnetic iron oxide nanoparticles; it should be noted that the kind of the magnetic nanoparticles is not limited, and can be applied to the formulation.
A preparation method of a p-hydroxyphenylalanine urine detection reagent magnetic nano-microsphere comprises the following steps:
preparing materials according to a formula;
the formula comprises the following components: 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant;
preparing mercury sulfate magnetic nano microspheres:
step 1, preparing a microsphere polymer shell substrate:
dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate;
step 2, microsphere polymer core layer solution:
mixing mercury sulfate with 24-46 parts of surfactant;
step 3, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercury sulfate magnetic nano microspheres; the method for wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; after the injection is finished, the curing is finished in a curing bath to obtain nano microspheres;
preparing mercurous nitrate magnetic nano microspheres:
step a, preparing a microsphere polymer shell substrate:
dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate;
step b, preparing a microsphere polymer core layer solution:
mercurous nitrate and 32-54 parts of surfactant;
step c, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercurous nitrate magnetic nano microspheres; the method for wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; after the injection is finished, the curing is finished in a curing bath to obtain nano microspheres;
preparing nickel sulfate magnetic nano microspheres:
in the step A, the step B is carried out,
dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate;
step B, microsphere polymer core layer solution:
mixing nickel sulfate and 4-12 parts of surfactant;
step C, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain nickel sulfate magnetic nano microspheres; the method for wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; after the injection is finished, the curing is finished in a curing bath to obtain nano microspheres;
preparing alpha-nitroso-beta-naphthol magnetic nano microspheres:
step I, preparing a microsphere polymer shell substrate:
dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate;
step II, preparing a microsphere polymer core layer solution:
mixing alpha-nitroso-beta-naphthol and 4-8 parts of surfactant;
step III, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain the alpha-nitroso-beta-naphthol magnetic nano-microsphere; the method for wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; after the injection is finished, the curing is finished in a curing bath to obtain nano microspheres;
mixing the mercury sulfate magnetic nano-microspheres, the mercurous nitrate magnetic nano-microspheres, the nickel sulfate magnetic nano-microspheres and the alpha-nitroso-beta-naphthol magnetic nano-microspheres according to the volume ratio of 2-3:2.2-4.4:1.6-2.2:0.8-1.2 to obtain the para-hydroxy phenylalanine urine detection reagent magnetic nano-microspheres. Preferably, the magnetic nano-microspheres of the reagent for detecting the p-hydroxy phenylalanine urine are obtained by mixing the magnetic nano-microspheres of the mercury sulfate, the magnetic nano-microspheres of the mercurous nitrate, the magnetic nano-microspheres of the nickel sulfate and the magnetic nano-microspheres of the alpha-nitroso-beta-naphthol according to the volume ratio of 2.2:3.6:1.8: 1.0.
The method for wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using the coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; and after the injection is finished, the nano microspheres are obtained by finishing the curing in a curing bath.
Experiments below prove that the detection reagent disclosed by the invention can maintain the stability of the reagent even in a harsh environment, improve the storage convenience and provide conditions for making portable equipment.
26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant. As an example, the acid-soluble chitosan is terephthalaldehyde crosslinked chitosan, and the surfactant is sodium secondary alkyl sulfate at a concentration of 1%. As an example, the magnetic nanoparticles include: iron oxide nanoparticles
Mixing mercury sulfate with 24-46 parts of surfactant; mercurous nitrate and 32-54 parts of surfactant; mixing nickel sulfate and 4-12 parts of surfactant; mixing nickel sulfate and 4-12 parts of surfactant; mixing alpha-nitroso-beta-naphthol and 4-8 parts of surfactant
Example 1: 26g of gelatin, 18g of acid-soluble chitosan, 0.6g of iron oxide nanoparticles, 60g of absolute ethyl alcohol, 12g of mercury sulfate, 24g of matched 1% secondary alkyl sodium sulfate, 56g of mercurous nitrate, 32g of matched 1% secondary alkyl sodium sulfate, 2g of nickel sulfate, 4g of matched 1% secondary alkyl sodium sulfate, 1g of alpha-nitroso-beta-naphthol and 4g of matched 1% secondary alkyl sodium sulfate.
Example 2: 34g of gelatin, 26g of acid-soluble chitosan, 0.8g of iron oxide nanoparticles, 90g of absolute ethyl alcohol, 18g of mercury sulfate, 38g of matched 1% secondary alkyl sodium sulfate, 62g of mercurous nitrate, 48g of matched 1% secondary alkyl sodium sulfate, 4g of nickel sulfate, 8g of matched 1% secondary alkyl sodium sulfate, 2g of alpha-nitroso-beta-naphthol and 6g of matched 1% secondary alkyl sodium sulfate.
Example 3: 42g of gelatin, 34g of acid-soluble chitosan, 1.2g of iron oxide nanoparticles, 120g of absolute ethyl alcohol, 24g of mercury sulfate, 46g of matched 1% secondary alkyl sodium sulfate, 68g of mercurous nitrate, 54g of matched 1% secondary alkyl sodium sulfate, 6g of nickel sulfate, 12g of matched 1% secondary alkyl sodium sulfate, 3g of alpha-nitroso-beta-naphthol and 8g of matched 1% secondary alkyl sodium sulfate.
The comparative example formulation included:
preparing a mercuric sulfate solution: adding 18g of mercuric sulfate into a 12mol/L sulfuric acid solution, stirring and dissolving, and then diluting to 100ml by using the 12mol/L sulfuric acid solution to obtain a colorless and transparent liquid for later use;
preparing a mercurous nitrate solution: adding 62g of mercurous nitrate into a 24mol/L nitric acid solution, diluting the dissolved mercurous nitrate to 100ml by using the 24mol/L nitric acid solution to obtain a green liquid, and standing the green liquid for later use after the green color of the solution is faded;
preparing a nickel sulfate solution: dissolving 4g of nickel sulfate into distilled water, and diluting the solution to 100ml by using the distilled water to obtain blue-green liquid for later use;
preparing an alpha-nitroso-beta-naphthol solution: dissolving 2g of alpha-nitroso-beta-naphthol in 100ml of 12mol/L nitric acid solution for later use;
mixing: and mixing a mercury sulfate solution, a mercurous nitrate solution, a nickel sulfate solution and an alpha-nitroso-beta-naphthol solution according to the volume ratio of 1.4:1.6:0.3:0.2 to obtain a comparative sample 1.
The reagent can react with tyrosine, tryptophan and derivatives thereof in urine and denatured protein containing the amino acid residues to generate precipitation with specific color, and the result is judged in an auxiliary manner according to a colorimetric board.
The magnetic nano-microspheres of mercury sulfate, the magnetic nano-microspheres of mercurous nitrate, the magnetic nano-microspheres of nickel sulfate and the magnetic nano-microspheres of alpha-nitroso-beta-naphthol prepared in examples 1 to 3 by the above method were mixed according to a volume ratio of 2.2:3.6:1.8:1.0 to obtain the magnetic nano-microspheres of p-hydroxy-phenylalanine urine detection reagent.
Experiment one: environmental tolerance test:
putting 6g of samples 1-3 into a sealed test tube, and performing simulated solarization experiments for 30 days and shaking experiments;
putting the comparative sample 1 into a sealed test tube, and performing the same simulated solarization experiment for 30 days and shaking experiment;
after 30 days, 6g of the sample of example 2 was designated sample 4; as comparative sample 2, prepared using the formulation of the comparative example;
dissolving the samples 1-4 by using a mixed solution of sulfuric acid and nitric acid for later use;
dripping two drops of the leucine solution into the sample 1-4 and the comparative sample 1-2 respectively to observe the color change condition;
| speed of color change | Degree of apparent discoloration | |
| Test sample 1 | Fast-acting toy | Is obvious |
| Test sample 2 | Fast-acting toy | Is obvious |
| Test sample 3 | Fast-acting toy | Is obvious |
| Test sample 4 | Fast-acting toy | Is obvious |
| Comparative sample 1 | Slow | Is not obvious |
| Comparative sample 2 | Fast-acting toy | Is obvious |
From the comparison of samples 1-3 and sample 4: the detection activity can still be maintained after the mode adopted by the invention passes through harsh environment, the color change speed and the obvious degree of color change are good, and the contrast sample 1 and the contrast sample 2 show that the sensitivity of the newly configured contrast sample is good, and the sensitivity degree of the contrast sample 1 is reduced after the harsh environment is used for a long time; the product of the invention has environmental tolerance capability, and can maintain stability and sensitivity even in harsh environment for a long time. The formula adopted by the microsphere polymer shell substrate is matched with the magnetic nanoparticles, so that microspheres carrying various reagents can be rapidly aggregated together when in use, the dissolving speed is improved, and the detection speed is not influenced.
Experiment two: performing an accuracy test;
taking urine of a patient in a cooperative hospital and urine of a healthy person;
taking the test samples 1-4 and the comparison samples 1-2 of the first experiment to respectively detect the urine of the patient and the urine of the healthy person, wherein the experiment results are as follows:
from the above results, it can be seen that only the results of the comparative sample 1 are inconsistent with the results of the hospital examinations, so that the method of the present invention can maintain the detection accuracy even after a long period of harsh environment, and can satisfy the conditions for manufacturing portable detection devices.
The invention provides a magnetic nano microsphere of a p-hydroxy phenylalanine urine detection reagent and a preparation method thereof, which can improve the stability of the detection reagent, do not need a complex preparation process, can be used for detection after acid liquid is mixed, are convenient to manufacture into portable detection equipment and improve the accuracy of urine detection.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (8)
1. The utility model provides a p hydroxy phenyl alanine urine detect reagent magnetism nanometer microballon which characterized in that includes: the formula comprises the following components:
26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant.
2. The magnetic nanosphere of para-hydroxy phenylalanine urine test reagent of claim 1, wherein said acid-soluble chitosan is terephthalaldehyde cross-linked chitosan.
3. The magnetic nanoparticle for the p-hydroxyphenylalanine urine detection reagent according to claim 1, wherein the magnetic nanoparticle comprises: iron oxide nanoparticles, superparamagnetic iron oxide nanoparticles.
4. The magnetic nanoparticle for the p-hydroxyphenylalanine urine detection reagent according to claim 1, wherein the surfactant is sodium secondary alkyl sulfate with a concentration of 1%.
5. A preparation method of a p-hydroxyphenylalanine urine detection reagent magnetic nano-microsphere is characterized by comprising the following steps:
preparing materials according to a formula;
the formula comprises the following components: 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta-naphthol and 64-120 parts of surfactant;
preparing mercury sulfate magnetic nano microspheres:
step 1, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step 2, microsphere polymer core layer solution:
mixing mercury sulfate with 24-46 parts of surfactant;
step 3, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercury sulfate magnetic nano microspheres;
preparing mercurous nitrate magnetic nano microspheres:
step a, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step b, preparing a microsphere polymer core layer solution:
mercurous nitrate and 32-54 parts of surfactant;
step c, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain mercurous nitrate magnetic nano microspheres;
preparing nickel sulfate magnetic nano microspheres:
in the step A, the step B is carried out,
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step B, microsphere polymer core layer solution:
mixing nickel sulfate and 4-12 parts of surfactant;
step C, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain nickel sulfate magnetic nano microspheres;
preparing alpha-nitroso-beta-naphthol magnetic nano microspheres:
step I, preparing a microsphere polymer shell substrate:
stirring gelatin, acid-soluble chitosan, magnetic nanoparticles and absolute ethyl alcohol, defoaming and curing to obtain a polymer shell substrate;
step II, preparing a microsphere polymer core layer solution:
mixing alpha-nitroso-beta-naphthol and 4-8 parts of surfactant;
step III, wrapping the microsphere polymer shell substrate with the microsphere polymer core layer solution by using a coaxial injector to obtain the alpha-nitroso-beta-naphthol magnetic nano-microsphere;
mixing the mercury sulfate magnetic nano-microspheres, the mercurous nitrate magnetic nano-microspheres, the nickel sulfate magnetic nano-microspheres and the alpha-nitroso-beta-naphthol magnetic nano-microspheres according to the volume ratio of 2-3:2.2-4.4:1.6-2.2:0.8-1.2 to obtain the para-hydroxy phenylalanine urine detection reagent magnetic nano-microspheres.
6. The method for preparing p-hydroxyphenylalanine urine detection reagent magnetic nanospheres according to claim 5,
the specific preparation method of the microsphere polymer shell substrate comprises the following steps: dissolving gelatin in water at 80-90 deg.C, stirring, adding acid soluble chitosan into gelatin solution, stirring, adding magnetic nanoparticles, adding anhydrous ethanol, stirring at 20-40 deg.C for dissolving, defoaming at 0.06-0.08MPa, and aging to obtain polymer shell substrate.
7. The preparation method of the magnetic nano-microsphere for the p-hydroxy-phenylalanine urine detection reagent of claim 5, wherein the magnetic nano-microsphere for the p-hydroxy-phenylalanine urine detection reagent is obtained by mixing the magnetic nano-microsphere for mercury sulfate, the magnetic nano-microsphere for mercurous nitrate, the magnetic nano-microsphere for nickel sulfate and the magnetic nano-microsphere for alpha-nitroso-beta-naphthol according to a volume ratio of 2.2:3.6:1.8: 1.0.
8. The preparation method of the magnetic nano-microsphere as the p-hydroxyphenylalanine urine detection reagent according to claim 5, wherein the method for wrapping the microsphere polymer shell substrate with the microsphere polymer core solution by using a coaxial injector comprises the following steps: placing a microsphere polymer shell substrate and a microsphere polymer core layer solution into a coaxial injector, wherein the inner diameter of a needle for placing the microsphere polymer shell substrate is 1.0-1.2mm, the flow rate of the solution is 6-10ml/h, the inner diameter of the needle for placing the microsphere polymer core layer solution is 0.6-1.0mm, the flow rate of the solution is 1.4-2.6ml/h, the injection voltage is 18-26kv, and the spraying distance is 4-8 cm; and after the injection is finished, the nano microspheres are obtained by finishing the curing in a curing bath.
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