CN112210547A - Mushroom bran crude enzyme preparation and preparation method thereof - Google Patents

Mushroom bran crude enzyme preparation and preparation method thereof Download PDF

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Publication number
CN112210547A
CN112210547A CN201910631301.7A CN201910631301A CN112210547A CN 112210547 A CN112210547 A CN 112210547A CN 201910631301 A CN201910631301 A CN 201910631301A CN 112210547 A CN112210547 A CN 112210547A
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China
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mushroom bran
preparation
drying
crude enzyme
fresh
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CN201910631301.7A
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Chinese (zh)
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闫景彩
黄丽琴
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Hunan Agricultural University
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Hunan Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01013—Manganese peroxidase (1.11.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01014—Lignin peroxidase (1.11.1.14)

Abstract

The invention relates to a mushroom bran crude enzyme preparation and a preparation method thereof. Selecting fresh, clean and mildew-free fungus bags after the mushrooms are harvested, removing coating materials, slightly hammering or rolling fungus bran blocks to obtain dispersed fresh fungus bran, and then carrying out vacuum packaging on the dispersed fresh fungus bran to obtain a finished product; or spreading and drying the dispersed fresh fungus chaff to reduce the water content to below 20 percent, and then carrying out vacuum packaging to obtain a finished product; or squeezing or pulping the dispersed fresh mushroom bran, filtering by 40-100 meshes, drying the filtrate by using a spray drying method/boiling granulation drying method/ultrafiltration method/or reduced pressure drying method to reduce the water content of the solid to below 15%, and then carrying out vacuum packaging to obtain the finished product. The main functional components of the mushroom bran crude enzyme preparation are lignin-degrading enzyme, cellulose-degrading enzyme and hemicellulose-degrading enzyme, and the mushroom bran crude enzyme preparation is mainly used for degrading lignin, cellulose and hemicellulose in biomass materials.

Description

Mushroom bran crude enzyme preparation and preparation method thereof
Technical Field
The invention relates to an enzyme preparation and a preparation method thereof, in particular to a crude enzyme preparation of mushroom bran, which is prepared by using fresh mushroom bran obtained after mushroom harvesting as a raw material through impurity removal, dispersion, vacuum packaging, or drying and vacuum packaging, or juice preparation, drying and vacuum packaging. The crude enzyme preparation mainly comprises the functional components of lignin hydrolase, cellulose hydrolase and hemicellulose hydrolase, and is mainly used for degrading lignin, cellulose and hemicellulose in biomass materials.
Background
Many species of edible fungi are capable of secreting not only cellulose, hemicellulases, but also ligninolytic enzymes. The ligninase, cellulase and hemicellulase activities of the mycelium were the highest among the various parts of the edible fungi. A large amount of hypha and various hydrolytic enzymes are distributed in the waste fungus chaff after the edible fungus is harvested. The annual output of the edible fungus chaff in China reaches 600-700 ten thousand tons, and the edible fungus chaff is a huge biological enzyme resource.
In the current fermentation production process aiming at degrading lignin, cellulose and hemicellulose in biomass, fungus live bacteria are generally directly utilized to ferment bottom materials, and the fermentation is aerobic, so that fermentation raw materials need to be disinfected, otherwise, the fermentation raw materials are difficult to mildew, so that the production process is high in cost, and the energy loss of the substrate is large. The research and production practice of fermenting biomass materials by using enzyme preparations such as ligninase, cellulase, hemicellulase and the like are also available, but the used enzyme preparations are mostly fine products produced specially, are usually high in price and inevitably influence popularization and application. The research of producing the crude enzyme preparation by using the edible fungus chaff for biomass fermentation has not been reported yet.
Disclosure of Invention
The object of the present invention is to provide an inexpensive crude enzyme preparation and a process for producing such a crude enzyme preparation. The crude enzyme preparation is prepared from edible fungus chaff serving as a raw material, the main functional components of the crude enzyme preparation are lignin hydrolase, cellulose hydrolase and hemicellulose hydrolase, and the crude enzyme preparation is mainly used for degrading lignin, cellulose and hemicellulose in a biomass material.
The technical scheme of the invention is as follows: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks to obtain dispersed fresh fungus chaff, and then completing the preparation process of the fungus chaff crude enzyme preparation by the following modes:
(1) filling the dispersed fresh mushroom bran into a clean plastic bag with or without an air valve, wherein the filling amount of the plastic bag without the air valve is about half of the maximum volume of the plastic bag, and the filling amount of the plastic bag with the air valve can reach more than two thirds of the maximum volume of the plastic bag, and then carrying out vacuum sealing to finish the preparation; or filling the dispersed fresh mushroom bran into other clean sealable containers with one-way exhaust valves, filling, compacting, and sealing to obtain the final product.
(2) And (3) placing the dispersed fresh mushroom bran in a ventilated and cool and dry place, spreading and airing until the moisture content is reduced to be below 20%, and then carrying out vacuum packaging to finish the preparation.
(3) Directly drying the dispersed fresh mushroom bran by using drying equipment, or drying under reduced pressure, or drying by using air flow, or drying by boiling to reduce the water content of the mushroom bran to below 15%, and then carrying out vacuum packaging to finish the preparation. The surface temperature of the fungus chaff particles is controlled below 45 ℃ in the drying process.
(4) Squeezing or pulping the dispersed fresh mushroom bran, filtering by 40-100 meshes, drying the filtrate by using a spray drying method, a boiling granulation drying method, an ultrafiltration method or a reduced pressure drying method to reduce the water content of a solid to below 15%, and then carrying out vacuum packaging to finish the preparation.
Detailed Description
The following examples are given to aid in the understanding of the present invention, but are not intended to limit the scope of the present invention.
Example 1: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks, filling the dispersed fresh fungus chaff blocks into a clean plastic bag with the filling amount being about half of the maximum volume of the plastic bag, and then carrying out vacuum sealing to finish the preparation.
Example 2: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks, filling the dispersed fresh fungus chaff blocks into a clean sealable plastic barrel with a one-way exhaust valve, compacting, and sealing to finish the preparation.
Example 3: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks, drying the dispersed fresh fungus chaff blocks under reduced pressure by using drying equipment to reduce the water content of the fungus chaff to below 15%, and then carrying out vacuum packaging to finish the preparation. The surface temperature of the fungus chaff particles is controlled below 45 ℃ in the drying process.
Example 4: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks, squeezing the dispersed fresh fungus chaff blocks, filtering by 40-100 meshes, drying filtrate by using a spray drying method to reduce the water content of solid matters to below 15%, and then carrying out vacuum packaging to finish the preparation.

Claims (3)

1. The mushroom bran crude enzyme preparation is characterized in that: the mushroom bran crude enzyme preparation is a mixed crude enzyme preparation prepared by taking edible mushroom bran as a main raw material, the main functional components of the preparation are lignin hydrolase, cellulose hydrolase and hemicellulose hydrolase, and the preparation is mainly used for degrading lignin, cellulose and hemicellulose in biomass materials.
2. The crude enzyme preparation of mushroom bran as claimed in claim 1, wherein the main raw material is various edible mushroom bran, and one crude enzyme preparation product of mushroom bran can be prepared from one or more edible seedling mushroom bran.
3. The method for preparing a crude enzyme preparation from mushroom bran according to claim 1, wherein the method comprises the steps of: selecting fresh, clean and mildew-free fungus chaff bags after mushroom harvest, removing coating materials, slightly hammering or rolling fungus chaff blocks to disperse the fungus chaff blocks to obtain dispersed fresh fungus chaff, and then completing the preparation process by the following modes:
firstly, filling dispersed fresh mushroom bran into a clean plastic bag with or without an air valve, wherein the filling amount of the plastic bag without the air valve is about half of the maximum volume of the plastic bag, and the filling amount of the plastic bag with the air valve can reach more than two thirds of the maximum volume of the plastic bag, and then carrying out vacuum sealing to finish the preparation; or filling the dispersed fresh mushroom bran into other clean sealable containers with one-way exhaust valves, filling, compacting, and sealing to obtain the final product;
secondly, placing the dispersed fresh mushroom bran in a ventilating, shady and dry place, spreading and airing until the moisture content is reduced to be below 20%, and then carrying out vacuum packaging to finish the preparation;
thirdly, directly drying the dispersed fresh mushroom bran by using drying equipment, or drying under reduced pressure, or drying by air flow, or drying by boiling to reduce the water content of the mushroom bran to below 15 percent, and then carrying out vacuum packaging to finish the preparation, wherein the surface temperature of the mushroom bran particles is controlled to be below 45 ℃ in the drying process;
and fourthly, squeezing or pulping the dispersed fresh mushroom bran, filtering by 40-100 meshes, drying the filtrate by using a spray drying method, a boiling granulation drying method, an ultrafiltration method or a reduced pressure drying method to reduce the water content of the solid matter to below 15%, and then carrying out vacuum packaging to finish the preparation.
CN201910631301.7A 2019-07-12 2019-07-12 Mushroom bran crude enzyme preparation and preparation method thereof Pending CN112210547A (en)

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Application Number Priority Date Filing Date Title
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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101423825A (en) * 2008-12-09 2009-05-06 嘉兴学院 Method for preparing cellulase from germ bran
CN103053858A (en) * 2013-01-31 2013-04-24 广东省农业科学院蚕业与农产品加工研究所 Flammulina velutipes fungus chaff feed and production method and application thereof
CN103740675A (en) * 2013-09-18 2014-04-23 明博医药技术开发（上海）有限公司 Method for producing cellulase
CN104694513A (en) * 2014-12-03 2015-06-10 江南大学 Method for producing ligninase by using edible mushroom stick
CN105779313A (en) * 2016-05-23 2016-07-20 沈阳德宝嘉泓农业科技有限公司 Method for preparing needle mushroom liquid strain by taking mushroom bran extracting solution as raw material
CN106119229A (en) * 2016-08-25 2016-11-16 重庆市中药研究院 The method separating cellulase from waste tremella cultivating material
CN106636018A (en) * 2016-12-28 2017-05-10 福建农林大学 Pleurotus geesteranus waste substrate crude enzyme preparation and application of preparation in fermentation production of ethanol
CN107083377A (en) * 2017-06-16 2017-08-22 信阳师范学院华锐学院 A kind of method that cellulose complex enzyme is extracted from agricultural by-products
CN107599112A (en) * 2017-09-15 2018-01-19 淮阴师范学院 Utilize the method for wood shavings production non-glue fibre board

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101423825A (en) * 2008-12-09 2009-05-06 嘉兴学院 Method for preparing cellulase from germ bran
CN103053858A (en) * 2013-01-31 2013-04-24 广东省农业科学院蚕业与农产品加工研究所 Flammulina velutipes fungus chaff feed and production method and application thereof
CN103740675A (en) * 2013-09-18 2014-04-23 明博医药技术开发（上海）有限公司 Method for producing cellulase
CN104694513A (en) * 2014-12-03 2015-06-10 江南大学 Method for producing ligninase by using edible mushroom stick
CN105779313A (en) * 2016-05-23 2016-07-20 沈阳德宝嘉泓农业科技有限公司 Method for preparing needle mushroom liquid strain by taking mushroom bran extracting solution as raw material
CN106119229A (en) * 2016-08-25 2016-11-16 重庆市中药研究院 The method separating cellulase from waste tremella cultivating material
CN106636018A (en) * 2016-12-28 2017-05-10 福建农林大学 Pleurotus geesteranus waste substrate crude enzyme preparation and application of preparation in fermentation production of ethanol
CN107083377A (en) * 2017-06-16 2017-08-22 信阳师范学院华锐学院 A kind of method that cellulose complex enzyme is extracted from agricultural by-products
CN107599112A (en) * 2017-09-15 2018-01-19 淮阴师范学院 Utilize the method for wood shavings production non-glue fibre board

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Application publication date: 20210112