CN112210514B - Method and device for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria - Google Patents

Method and device for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria Download PDF

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CN112210514B
CN112210514B CN202011104319.0A CN202011104319A CN112210514B CN 112210514 B CN112210514 B CN 112210514B CN 202011104319 A CN202011104319 A CN 202011104319A CN 112210514 B CN112210514 B CN 112210514B
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nitrifying bacteria
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aerobic denitrifying
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CN112210514A (en
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李志弘
张保安
杨秋婵
毛航球
刘静兰
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Guangdong Zhongwei Environmental Protection Biotechnology Co ltd
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Abstract

The invention relates to the technical field of microbial culture, in particular to a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria, which comprises the following steps: 1) adding the nitrifying bacteria nutrient solution and a micro-carrier into a nitrifying bacteria pool, and inoculating nitrifying bacteria strains for culture; 2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool, and supplementing nutrient solution and microcarriers; 3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool into a second nitrifying bacteria pool, and repeating the operation in the step 2) to obtain nitrifying bacteria liquid; 4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool into an aerobic denitrifying bacteria pool, and inoculating aerobic denitrifying bacteria seed liquid; 5) overflowing the cultured aerobic denitrifying bacteria liquid into a flocculation sedimentation tank, aerating, discharging thallus sediment, and preparing to obtain an aerobic denitrifying bacteria product. Compared with a method for culturing nitrifying bacteria or aerobic denitrifying bacteria independently, the method has the advantages of high cost, high thallus yield and high total nitrogen removal rate.

Description

Method and device for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria
Technical Field
The invention relates to the technical field of microbial culture, in particular to a method and a device for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria.
Background
In an ecological environment, the decomposition of pollutants is affected due to the damage of a micro-ecological system, and the accumulation of the pollutants causes ecological problems such as soil pollution, black and odorous water, eutrophication of the water and the like. In addition, in the treatment of industrial water, the proportion of nutrient components in the water body is disordered, so that the aim of standard discharge is difficult to achieve by using a common microbial treatment process. Therefore, the targeted additional high-efficiency strains can effectively improve the ecological restoration and the biochemical treatment capacity of the sewage treatment system.
The Composite Nitrifying Bacteria (CNB) comprise nitrosobacteria (AOB) capable of oxidizing ammonia nitrogen into nitrite and nitrifying bacteria (NOB) capable of oxidizing nitrite into nitrate, and the CNB can utilize energy assimilation inorganic carbon source generated by oxidizing ammonia nitrogen to perform chemoautotrophy, and belongs to autotrophy bacteria. In high-concentration ammonia nitrogen wastewater such as slaughtering, breeding, circuit boards and the like, the C/N ratio is not balanced, and the ammonia nitrogen hardly reaches the standard due to the low content of nitrobacteria by using a traditional common activated sludge method or a contact oxidation method; the slow degradation of ammonia nitrogen can be caused by insufficient content of CNB during the ecological restoration of the black and odorous water body. Therefore, the CNB is added to improve the nitrification capacity in the system, so that the degradation capacity of ammonia nitrogen can be obviously improved.
Patent application No. CN110551657A discloses that in the preparation method of a solid composite nitrobacteria agent, the growth process of nitrobacteria favors adherent growth, so that the addition of a microcarrier with large specific surface area during the culture process of the composite nitrobacteria is beneficial to the growth of the nitrobacteria.
Aerobic Denitrifying Bacteria (ADB) is a type of microorganism that can reduce nitrate to nitrogen under Aerobic conditions, and ADB is a species that requires an organic carbon source for heterotrophic denitrification. In addition to being oxidized by oxygen, the electrons and reducing hydrogen generated by the decomposition of organic substances in aerobic conditions can also be used for the reduction of nitrate and nitrite. ADB can play a good role when nitrate nitrogen needs to be removed under aerobic conditions.
In the traditional CNB culture process, a large amount of culture solution containing high-concentration nitrate is produced, if the culture solution is directly discharged as sewage, the culture solution obviously has great pollution and waste, and the nitrate produced by CNB culture can be effectively prevented from being polluted and wasted as a nutrient source for ADB culture. Patent application No. CN104611246A discloses a method for synchronously culturing nitrobacteria and aerobic denitrifying bacteria, which introduces a method for simultaneously culturing CNB and ADB by using a membrane module to divide a reactor into two culture areas. In this method, the nutrient solutions used for culturing the two bacterial cells are mixed together completely. Because CNB is autotrophic bacteria, the CNB can grow by oxidizing ammonia nitrogen into nitrate to generate energy in the culture process, and the CNB can assimilate inorganic carbon to synthesize life substances; and ADB is heterotrophic bacteria, so that an organic carbon source is required to be added for ADB culture, when the organic carbon source and ammonia nitrogen exist in the nutrient solution at the same time, mixed bacteria in the environment are easily polluted, the mass propagation of the mixed bacteria can inhibit the growth of CNB, so that nitrate in the nutrient solution cannot be continuously generated, and the growth of ADB is influenced. Finally, the concentration of the effective bacteria of the obtained bacterial liquid is low and the product quality is poor due to the pollution of mixed bacteria.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria, compared with the method for culturing nitrifying bacteria or aerobic denitrifying bacteria independently, the method has more advantages in cost, in the culture method, a first nitrobacteria pond, a second nitrobacteria pond and an aerobic denitrifying bacteria pond which are connected are arranged to culture nitrobacteria and aerobic denitrifying bacteria in a partitioned way and organically combine the nitrobacteria and the aerobic denitrifying bacteria together, CNB and ADB are cultured in a partitioned way, the condition that heterotrophic bacteria are polluted due to the absence of organic carbon in the culture stage of the composite nitrobacteria is ensured, the nitrogen source of nutrient solution in a tank body is mainly nitrate nitrogen and nitrite nitrogen due to extremely low ammonia nitrogen concentration when the composite nitrobacteria enters the aerobic denitrifying bacteria stage, therefore, the directional selectivity is provided for culturing the aerobic denitrifying bacteria so as to prevent the pollution of other mixed bacteria and ensure that the bacterial liquid obtained by culturing in each area is the target bacterial strain.
The invention also aims to provide a device for synchronously culturing the composite nitrobacteria and the aerobic denitrifying bacteria, which has more advantages in cost compared with a device for independently culturing the nitrobacteria or the aerobic denitrifying bacteria.
The purpose of the invention is realized by the following technical scheme: a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing nitrobacteria nutrient solution, adding the nitrobacteria nutrient solution into a first nitrobacteria pool and a second nitrobacteria pool respectively, adding microcarriers into the first nitrobacteria pool and the second nitrobacteria pool, inoculating nitrobacteria strains according to 5-10% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 5-10 d;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool, and when the nitrification activity of the thalli reaches 30-40mg (NH)3After N)/(L.h), discharging 50-70% of the bacterial liquid in the pool to prepare a product, and simultaneously supplementing nutrient solution and microcarrier with the same concentration as the nutrient solution in the step 1) according to the volume of the discharged bacterial liquid to continue culturing;
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool into a second nitrifying bacteria pool, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria tank in the step 3) into an aerobic denitrifying bacteria tank, controlling the ammonia nitrogen concentration of overflow liquid flowing into the aerobic denitrifying bacteria tank to be less than 2mg/L, collecting the overflow liquid, adding NaAC and HAc according to the conversion of 14.8-15.2 times of the total nitrogen concentration in the nitrifying bacteria nutrient solution to form a composite carbon source when the collected overflow liquid reaches 68-72% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid of which the volume is 2-10% of the volume of the overflow liquid, and starting aeration for culturing;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank in the step 4) into a flocculation sedimentation tank, adding PAC and PAM respectively after the liquid level of the overflowing liquid in the flocculation sedimentation tank rises to a certain height, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished, discharging supernatant, discharging the residual bacteria sediment through a bacteria discharge outlet at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation, wherein the concentration of the discharged bacteria sediment bacteria can reach 5 multiplied by 109cfu/g or more;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
The culture method has more advantages in cost compared with a method for independently culturing nitrifying bacteria or aerobic denitrifying bacteria, the first nitrifying bacteria pool, the second nitrifying bacteria pool and the aerobic denitrifying bacteria pool which are connected are arranged in the culture method, the nitrifying bacteria and the aerobic denitrifying bacteria are cultured in a partitioned mode and organically combined together, the CNB and the ADB are cultured in a partitioned mode, the situation that heterotrophic bacteria are polluted due to the fact that organic carbon does not exist in the composite nitrifying bacteria during the culture stage is guaranteed, when the composite nitrifying bacteria enter the aerobic denitrifying bacteria stage, due to the fact that the ammonia nitrogen concentration is extremely low, the nitrogen source of nutrient solution in a tank body is mainly nitrate nitrogen and nitrite nitrogen, the oriented selectivity is achieved for culturing the aerobic denitrifying bacteria, the pollution of other mixed bacteria is prevented, and the bacterium liquid obtained by culturing in each region is guaranteed to be the target strain. Wherein, nitrobacteria carry out nitration reaction by using ammonia nitrogen in the culture medium, and the generated nitrate nitrogen and nitrite nitrogen can enter an aerobic denitrifying bacteria pool to be used as nutrient substances of the aerobic denitrifying bacteria; meanwhile, the reduction of the product inhibition is beneficial to improving the process and effect of the nitration reaction, and the two are mutually supplemented and promoted, so that the efficient growth of the nitrifying bacteria and the denitrifying bacteria is realized, and the yield of the thallus is improved.
Preferably, in step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000041
preferably, in the step 1), the microcarrier is at least one of zeolite powder with 100-mesh and 500-mesh, perlite, diatomite, volcanic rock and double-flying powder; in the step 1), adding nitrobacteria for supplementing after the pH value in the first nitrobacteria pool is reduced to below 7.0, and maintaining the pH value in the first nitrobacteria pool to be 7.5-8.2.
The microcarrier adopted in the invention has large specific surface area, can increase the culture area, can enable the culture process to achieve homogeneous culture, efficiently utilizes the culture medium and reduces the chance of pollution; the pH value in the first nitrifying bacteria pool is maintained to be 7.5-8.2 by a material supplementing mode, so that the rapid growth of bacteria is facilitated, the efficiency of culturing the bacteria is improved, and the problems of pollution and waste caused by the discharge of nitrate generated in the production process of the composite nitrifying bacteria are solved.
Preferably, the nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000051
preferably, in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
Preferably, in the step 4), the complex carbon source is prepared by mixing NaAC and HAc in a molar ratio of 1: 0.8-1.2, wherein the mass concentration of the composite carbon source is 10-20 times of the total nitrogen concentration of the nitrobacteria feed liquid.
In the invention, the pH value is increased in the process of culturing the aerobic denitrifying bacteria, so that the use of the carbon source solution containing HAc can play a role in controlling the pH value in the aerobic denitrifying bacteria pool; while the organic carbon source is supplemented in a batch or continuous manner during the supplementation of the carbon source solution, the feeding is preferably carried out according to the consumption rate of the carbon source so as to reduce the influence of the organic carbon source on the nitrified sludge.
Preferably, in the step 5), the concentration of the PAM is 0.05-0.2%, and the concentration of the PAC is 2-10%. In the step 5), the flow rate of PAC is 5-10mL/min when PAC is added, and the flow rate of PAM is 3-10mL/min when PAM is added. In the step 5), PAC2-10min is continuously added at 5-10mL/min, and PAM5-10s is continuously added at 5-10 mL/min.
The invention also provides a device for the method for synchronously culturing the composite nitrifying bacteria and the aerobic denitrifying bacteria, which comprises the following steps:
the device comprises a biochemical reaction area and a feed supplement area communicated with the biochemical reaction area, wherein the biochemical reaction area comprises an air compression device, an aerobic denitrifying bacteria pool, a flocculation sedimentation pool, a first nitrifying bacteria pool, a second nitrifying bacteria pool and a plurality of aeration pipes, the second nitrifying bacteria pool is arranged on one side of the first nitrifying bacteria pool and communicated with the upper end of the first nitrifying bacteria pool, the aerobic denitrifying bacteria pool is arranged on one side of the second nitrifying bacteria pool and communicated with the second nitrifying bacteria pool, the flocculation sedimentation pool is arranged on one side of the aerobic denitrifying bacteria pool and communicated with the aerobic denitrifying bacteria pool, the aeration pipes are arranged at the bottoms of the first nitrifying bacteria pool, the second nitrifying bacteria pool and the aerobic denitrifying bacteria pool, the aeration pipes are inserted into the flocculation sedimentation pool from top to bottom in the flocculation sedimentation pool, and are communicated with the air compression device, the air compression device is arranged on the other side of the nitrifying bacteria pool.
Preferably, the feed supplementing zone comprises a nitrifying bacteria-containing feed supplementing tank, an aerobic denitrifying bacteria carbon source tank, a PAM storage tank and a PAC storage tank, the nitrifying bacteria-containing feed supplementing tank is communicated with the first nitrifying bacteria pool, the aerobic denitrifying bacteria carbon source tank is communicated with the second nitrifying bacteria pool, the PAM storage tank is communicated with the aerobic denitrifying bacteria pool, the PAC storage tank is communicated with the flocculation sedimentation pool, the device is provided with a control electric cabinet, the stirring devices are all connected with the control electric cabinet, and the control electric cabinet is positioned on one side of the flocculation sedimentation pool; more preferably, a third water pump is arranged between the nitrifying bacteria-containing feed tank and the first nitrifying bacteria pool, a second water pump is arranged between the PAC storage tank and the flocculation sedimentation tank, a first water pump is arranged between the PAM storage tank and the flocculation sedimentation tank, and a fourth water pump is arranged at a water inlet at the communication position of the aerobic denitrification bacteria pool and the second nitrifying bacteria pool; a drainage pipeline for discharging thallus sediments is arranged at the bottom of the flocculation sedimentation tank, and a first drainage port, a second drainage port and an overflow port are sequentially arranged on the side wall of the flocculation sedimentation tank from the drainage pipeline to the top; still be equipped with the pH electrode in the first nitrobacteria pond, the pH electrode with the control electricity cabinet is connected, be equipped with liquid level electrode in the flocculation and precipitation pond, liquid level electrode is located overflow mouth department, just liquid level electrode with the control electricity cabinet is connected
The invention has the beneficial effects that: the culture method has more advantages in cost compared with a method for independently culturing nitrifying bacteria or aerobic denitrifying bacteria, the culture method organically combines the nitrifying bacteria and the aerobic denitrifying bacteria through arranging the first nitrifying bacteria pool, the second nitrifying bacteria pool and the aerobic denitrifying bacteria pool which are connected, and carries out the partition culture of CNB and ADB, so that the condition that heterotrophic bacteria are polluted due to the absence of organic carbon in the culture stage of the composite nitrifying bacteria is ensured, and when the composite nitrifying bacteria enters the aerobic denitrifying bacteria stage, because the ammonia nitrogen concentration is extremely low, a nutrient solution nitrogen source in a tank body mainly comprises nitrate nitrogen and nitrite nitrogen, the oriented selectivity for culturing the aerobic denitrifying bacteria is also realized, so that the pollution of other bacteria is prevented, and the bacteria liquid obtained by culturing in each region is ensured to be a target strain. Wherein, nitrobacteria carry out nitration reaction by using ammonia nitrogen in the culture medium, and the generated nitrate nitrogen and nitrite nitrogen can enter an aerobic denitrifying bacteria pool to be used as nutrient substances of the aerobic denitrifying bacteria; meanwhile, the reduction of the product inhibition is beneficial to improving the process and effect of the nitration reaction, and the two are mutually supplemented and promoted, so that the efficient growth of the nitrifying bacteria and the denitrifying bacteria is realized, and the yield of the thallus is improved.
Compared with a device for culturing nitrobacteria or aerobic denitrifying bacteria independently, the device has more advantages in cost, and by organically combining the zonal culture of the nitrobacteria and the aerobic denitrifying bacteria, the device avoids the mixed bacteria pollution caused by the fact that the nutrient solution has no directional selectivity when the nitrobacteria and the aerobic denitrifying bacteria are cultured in the same tank, and ensures that the bacteria liquid obtained by culturing in each zone is the target bacteria.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a schematic view showing the structures of a biochemical reaction region and a feed region of the present invention.
The reference signs are: 1-biochemical reaction zone, 11-air compression device, 12-aerobic denitrifying bacteria pool, 13-flocculation sedimentation tank, 131-drainage pipe, 132-first drainage port, 133-second drainage port, 134-overflow port, 135-liquid level electrode, 14-first nitrifying bacteria pool, 141-stirring device, 142-pH electrode, 15-second nitrifying bacteria pool, 16-aeration pipe, 17-valve, 2-material feeding zone, 21-nitrifying bacteria material feeding tank, 22-aerobic denitrifying bacteria carbon source tank, 23-PAM storage tank, 24-PAC storage tank, 3-control electric cabinet, 41-first water pump, 42-second water pump, 43-third water pump and 44-fourth water pump.
Detailed Description
For the understanding of those skilled in the art, the present invention will be further described with reference to the following examples and accompanying fig. 1-2, which are not intended to limit the present invention.
Example 1
Referring to fig. 1-2, a device for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises a biochemical reaction zone 1 and a material supplementing zone 2 communicated with the biochemical reaction zone 1, wherein the biochemical reaction zone 1 comprises an air compression device 11, an aerobic denitrifying bacteria tank 12, a flocculation sedimentation tank 13, a first nitrifying bacteria tank 14, a second nitrifying bacteria tank 15 and a plurality of aeration pipes 16, the second nitrifying bacteria tank 15 is arranged at one side of the first nitrifying bacteria tank 14 and communicated with the upper end of the first nitrifying bacteria tank 14, the aerobic denitrifying bacteria tank 12 is arranged at one side of the second nitrifying bacteria tank 15 and communicated with the second nitrifying bacteria tank 15, the flocculation sedimentation tank 13 is arranged at one side of the aerobic denitrifying bacteria tank 12 and communicated with the aerobic denitrifying bacteria tank 12, the aeration pipes 16 are all arranged at the bottoms of the first nitrifying bacteria tank 14, the second nitrifying bacteria tank 15 and the aerobic denitrifying bacteria tank 12, the aeration pipe 16 is inserted into the flocculation sedimentation tank 13 from top to bottom in the flocculation sedimentation tank 13, the aeration pipes 16 are all communicated with the air compression device 11, and the air compression device 11 is arranged at the other side of the nitrifying bacteria tank. The feed supplement zone 2 comprises a nitrifying bacteria-containing feed supplement tank 21, an aerobic denitrifying bacteria carbon source tank 22, a PAM storage tank 23 and a PAC storage tank 24, the nitrifying bacteria-containing feed supplement tank 21 is communicated with the first nitrifying bacteria pool 14, the aerobic denitrifying bacteria carbon source tank 22 is communicated with the second nitrifying bacteria pool 15, the PAM storage tank 23 is communicated with the aerobic denitrifying bacteria pool 12, the PAC storage tank 24 is communicated with the flocculation sedimentation pool 13, the device is provided with a control electric cabinet 3, the stirring devices 141 are all connected with the control electric cabinet 3, and the control electric cabinet 3 is positioned on one side of the flocculation sedimentation pool 13; furthermore, a third water pump 43 is arranged between the nitrifying bacteria-containing material supplementing tank 21 and the first nitrifying bacteria pool 14, a second water pump 42 is arranged between the PAC storage tank 24 and the flocculation sedimentation pool 13, a first water pump 41 is arranged between the PAM storage tank 23 and the flocculation sedimentation pool 13, and a fourth water pump 44 is arranged at a water inlet at the communication position of the aerobic denitrifying bacteria pool 12 and the second nitrifying bacteria pool 15; a drainage pipe 131 for discharging thallus sediments is arranged at the bottom of the flocculation sedimentation tank 13, and a first drainage port 132, a second drainage port 133 and an overflow port 134 are sequentially arranged on the side wall of the flocculation sedimentation tank 13 from the drainage pipe 131 upwards; still be equipped with pH electrode 142 in the first nitrobacteria pond 14, pH electrode 142 with control electric cabinet 3 is connected, be equipped with liquid level electrode 135 in the flocculation and precipitation pond 13, liquid level electrode 135 is located overflow mouth 134 department, just liquid level electrode 135 with control electric cabinet 3 is connected.
A method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing a nitrifying bacteria nutrient solution, respectively adding the nitrifying bacteria nutrient solution into a first nitrifying bacteria pool 14 and a second nitrifying bacteria pool 15, adding microcarriers into the first nitrifying bacteria pool 14 and the second nitrifying bacteria pool 15, inoculating nitrifying bacteria strains according to 5% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 5 d;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool 14, when the nitrification activity of the thalli reaches 30mg (NH)3After the N)/(L.h), discharging 50% of the bacteria liquid in the pool to prepare a product, and simultaneously supplementing nutrient liquid and microcarrier with the same volume as the discharged bacteria liquid from a nitrifying bacteria supplementing tank 21 through a third water pump 43 to continue culturing, wherein the supplemented nutrient liquid and microcarrier have the same concentration as the nutrient liquid and microcarrier in the step 1);
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool 14 into the second nitrifying bacteria pool 15, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool 15 in the step 3) into the aerobic denitrifying bacteria pool 12, controlling the ammonia nitrogen concentration of the overflowing liquid flowing into the aerobic denitrifying bacteria pool 12 to be less than 2mg/L, collecting the overflowing liquid, adding NaAC and HAc to form a composite carbon source according to the 14.8-fold conversion of the total nitrogen concentration in the nitrifying bacteria nutrient solution when the collected overflowing liquid reaches 68% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid of 2% of the volume of the overflowing liquid, starting aeration for culturing, after starting aeration culture of the aerobic denitrifying bacteria pool 12, communicating a third water pump 43 and a fourth water pump 44, starting the fourth water pump 44 simultaneously when the third water pump 43 runs, wherein the third water pump 43 and the fourth water pump 44 have the same model and size, and the feed liquid replenishing speed is the same;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank 12 in the step 4) into the flocculation sedimentation tank 13, triggering the first water pump 41 and the second water pump 42 to respectively add PAC and PAM when the liquid level of the overflowing liquid in the flocculation sedimentation tank 13 rises to the liquid level electrode 135, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished to discharge supernatant, discharging the residual bacteria sediment through a water discharge pipe 131 at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
In the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000101
in the step 1), the microcarrier is zeolite powder with 100 meshes.
In the step 1), the third water pump 43 is controlled by controlling the electric cabinet 3, and when the pH value in the first nitrifying bacteria tank 14 is reduced to below 7.0, the third water pump 43 is started to feed the nitrifying bacteria for feeding, so as to maintain the pH value in the first nitrifying bacteria tank 14 at 7.5.
The nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000102
in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool 14 comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
In the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 0.8, wherein the mass concentration of the composite carbon source is 10 times of the total nitrogen concentration of the nitrifying bacteria feed liquid.
In the step 5), the concentration of PAM is 0.05%, and the concentration of PAC is 2%.
In the step 5), the flow rate of PAC when PAC is added is 5mL/min, and the flow rate of PAM when PAM is added is 300 mL/min.
In said step 5), PAC was continuously added at 5mL/min for 2min, while PAM10s was continuously added at 5 mL/min.
Example 2
This embodiment differs from embodiment 1 described above in that:
a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing a nitrifying bacteria nutrient solution, respectively adding the nitrifying bacteria nutrient solution into a first nitrifying bacteria pool 14 and a second nitrifying bacteria pool 15, adding microcarriers into the first nitrifying bacteria pool 14 and the second nitrifying bacteria pool 15, inoculating nitrifying bacteria strains according to 7% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 7 days;
2) detecting the nitrification activity of the bacteria in the first nitrifying bacteria tank 14, when the nitrification activity of the bacteria reaches 33mg (NH)3after-N)/(Lh), discharging 55% of bacterial liquid in the pool for preparation to obtain a product, and simultaneously feeding nitrobacteria by a third water pump 43The tank 21 is supplemented with nutrient solution and microcarrier with the same volume as the discharged bacterial liquid for continuous culture, and the supplemented nutrient solution and microcarrier have the same concentration as the nutrient solution and microcarrier in the step 1);
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool 14 into the second nitrifying bacteria pool 15, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool 15 in the step 3) into the aerobic denitrifying bacteria pool 12, controlling the ammonia nitrogen concentration of the overflowing liquid flowing into the aerobic denitrifying bacteria pool 12 to be less than 2mg/L, collecting the overflowing liquid, adding NaAC and HAc to form a composite carbon source according to the 14.9-fold conversion of the total nitrogen concentration in the nitrifying bacteria nutrient solution when the collected overflowing liquid reaches 69% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid with the volume of 4% of the overflowing liquid, starting aeration for culturing, after starting aeration culture of the aerobic denitrifying bacteria pool 12, communicating a third water pump 43 and a fourth water pump 44, starting the fourth water pump 44 simultaneously when the third water pump 43 runs, wherein the third water pump 43 and the fourth water pump 44 have the same model and the same size, and the feed liquid replenishing speed are the same;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank 12 in the step 4) into the flocculation sedimentation tank 13, triggering the first water pump 41 and the second water pump 42 to respectively add PAC and PAM when the liquid level of the overflowing liquid in the flocculation sedimentation tank 13 rises to the liquid level electrode 135, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished to discharge supernatant, discharging the residual bacteria sediment through a water discharge pipe 131 at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
In the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000121
in the step 1), the microcarrier is perlite with 200 meshes.
In the step 1), the third water pump 43 is controlled by controlling the electric cabinet 3, and when the pH value in the first nitrifying bacteria tank 14 is reduced to below 7.0, the third water pump 43 is started to feed the nitrifying bacteria for feeding, so as to maintain the pH value in the first nitrifying bacteria tank 14 to be 7.6.
The nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000131
in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool 14 comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
In the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 0.9, wherein the mass concentration of the composite carbon source is 13 times of the total nitrogen concentration of the nitrifying bacteria feed liquid.
In the step 5), the concentration of PAM is 0.075% and the concentration of PAC is 10%.
In the step 5), the flow rate of PAC when PAC is added is 7mL/min, and the flow rate of PAM when PAM is added is 7 mL/min.
In said step 5), PAC was continuously added at 6mL/min for 4min, while PAM10s was continuously added at 7 mL/min.
Other technical features of this embodiment are the same as those of embodiment 1, and will not be described again.
Example 3
This embodiment differs from embodiment 1 described above in that:
a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing a nitrifying bacteria nutrient solution, respectively adding the nitrifying bacteria nutrient solution into a first nitrifying bacteria pool 14 and a second nitrifying bacteria pool 15, adding microcarriers into the first nitrifying bacteria pool 14 and the second nitrifying bacteria pool 15, inoculating nitrifying bacteria strains according to 8% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 8 days;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool 14, and when the nitrification activity of the thalli reaches 35mg (NH)3After the N)/(L.h), discharging 60% of the bacterial liquid in the pool to prepare a product, and simultaneously supplementing nutrient solution and microcarrier with the same volume as the discharged bacterial liquid from a nitrifying bacteria supplementing tank 21 through a third water pump 43 to continue culturing, wherein the supplemented nutrient solution and microcarrier have the same concentration as the nutrient solution and microcarrier in the step 1);
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool 14 into the second nitrifying bacteria pool 15, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool 15 in the step 3) into the aerobic denitrifying bacteria pool 12, controlling the ammonia nitrogen concentration of the overflowing liquid flowing into the aerobic denitrifying bacteria pool 12 to be less than 2mg/L, collecting the overflowing liquid, adding NaAC and HAc to form a composite carbon source according to 15 times of the total nitrogen concentration in the nitrifying bacteria nutrient solution when the collected overflowing liquid reaches 70% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid with the volume of 6% of the overflowing liquid, starting aeration for culturing in the aerobic denitrifying bacteria pool 12, communicating a third water pump 43 and a fourth water pump 44 after starting aeration culture, simultaneously starting the fourth water pump 44 when the third water pump 43 runs, and ensuring that the model sizes of the third water pump 43 and the fourth water pump 44 are the same and the feed liquid replenishing speed is the same;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank 12 in the step 4) into the flocculation sedimentation tank 13, triggering the first water pump 41 and the second water pump 42 to respectively add PAC and PAM when the liquid level of the overflowing liquid in the flocculation sedimentation tank 13 rises to the liquid level electrode 135, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished to discharge supernatant, discharging the residual bacteria sediment through a water discharge pipe 131 at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
In the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000141
Figure BDA0002726437650000151
in the step 1), the microcarrier is diatomite with 300 meshes.
In the step 1), the third water pump 43 is controlled by controlling the electric cabinet 3, and when the pH value in the first nitrifying bacteria tank 14 is reduced to below 7.0, the third water pump 43 is started to feed the nitrifying bacteria for feeding, so as to maintain the pH value in the first nitrifying bacteria tank 14 to be 7.8.
The nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000152
in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool 14 comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
In the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 1.0, wherein the mass concentration of the composite carbon source is 15 times of the total nitrogen concentration of the nitrifying bacteria feed liquid.
In the step 5), the concentration of adding PAM is 0.1%, and the concentration of adding PAC is 3.5%.
In the step 5), the flow rate of PAC when PAC is added is 8mL/min, and the flow rate of PAM when PAM is added is 2 mL/min.
In said step 5), PAC was continuously added at 8mL/min for 6min, while PAM10s was continuously added at 2 mL/min.
Other technical features of this embodiment are the same as those of embodiment 1, and will not be described again.
Example 4
This embodiment differs from embodiment 1 described above in that:
a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing a nitrifying bacteria nutrient solution, respectively adding the nitrifying bacteria nutrient solution into a first nitrifying bacteria pool 14 and a second nitrifying bacteria pool 15, adding microcarriers into the first nitrifying bacteria pool 14 and the second nitrifying bacteria pool 15, inoculating nitrifying bacteria strains according to 9% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 9 days;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool 14, when the nitrification activity of the thalli reaches 38mg (NH)3After the N)/(L.h), discharging 65% of the bacteria liquid in the pool to prepare a product, and simultaneously supplementing nutrient liquid and microcarrier with the same volume as the discharged bacteria liquid from a nitrifying bacteria supplementing tank 21 through a third water pump 43 to continue culturing, wherein the supplemented nutrient liquid and microcarrier have the same concentration as the nutrient liquid and microcarrier in the step 1);
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool 14 into the second nitrifying bacteria pool 15, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool 15 in the step 3) into the aerobic denitrifying bacteria pool 12, controlling the ammonia nitrogen concentration of the overflowing liquid flowing into the aerobic denitrifying bacteria pool 12 to be less than 2mg/L, collecting the overflowing liquid, adding NaAC and HAc to form a composite carbon source according to the conversion of 15.1 times of the total nitrogen concentration in the nitrifying bacteria nutrient solution when the collected overflowing liquid reaches 78% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid of 8% of the volume of the overflowing liquid, starting aeration for culturing, after starting aeration culture of the aerobic denitrifying bacteria pool 12, communicating a third water pump 43 and a fourth water pump 44, starting the fourth water pump 44 simultaneously when the third water pump 43 runs, wherein the third water pump 43 and the fourth water pump 44 have the same model and the same size, and the feed liquid replenishing speed is the same;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank 12 in the step 4) into the flocculation sedimentation tank 13, triggering the first water pump 41 and the second water pump 42 to respectively add PAC and PAM when the liquid level of the overflowing liquid in the flocculation sedimentation tank 13 rises to the liquid level electrode 135, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished to discharge supernatant, discharging the residual bacteria sediment through a water discharge pipe 131 at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
In the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000171
in the step 1), the microcarrier is volcanic rock with 400 meshes.
In the step 1), the third water pump 43 is controlled by the control electric cabinet 3, and when the pH value in the first nitrifying bacteria pool 14 is reduced to below 7.0, the third water pump 43 is started to feed the nitrifying bacteria for feeding, so as to maintain the pH value in the first nitrifying bacteria pool 14 to be 8.
The nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000172
Figure BDA0002726437650000181
in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool 14 comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
In the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 1.1, wherein the mass concentration of the composite carbon source is 18 times of the total nitrogen concentration of the nitrifying bacteria feed liquid.
In the step 5), the concentration of PAM is 0.15%, and the concentration of PAC is 4.2%.
In the step 5), the flow rate of PAC when PAC is added is 9mL/min, and the flow rate of PAM when PAM is added is 3 mL/min.
In said step 5), PAC was continuously added at 9mL/min for 8min, while PAM10s was continuously added at 3 mL/min.
Other technical features of this embodiment are the same as those of embodiment 1, and will not be described again.
Example 5
This embodiment differs from embodiment 1 described above in that:
a method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria comprises the following steps:
1) preparing a nitrifying bacteria nutrient solution, respectively adding the nitrifying bacteria nutrient solution into a first nitrifying bacteria pool 14 and a second nitrifying bacteria pool 15, adding microcarriers into the first nitrifying bacteria pool 14 and the second nitrifying bacteria pool 15, inoculating nitrifying bacteria strains according to 10% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 10 days;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool 14, and when the nitrification activity of the thalli reaches 40mg (NH)3After the ratio of-N to the L.h), 70 percent of the bacteria liquid in the discharging tank is discharged for preparation to obtain a product, meanwhile, a nutrient solution and a microcarrier with the same volume as the discharged bacteria liquid are supplemented from the nitrifying bacteria supplementing tank 21 through a third water pump 43 for continuous culture, and the supplemented nutrient solution and microcarrier are addedThe concentrations of the nutrient solution and the microcarrier are the same as those of the nutrient solution and the microcarrier in the step 1);
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool 14 into the second nitrifying bacteria pool 15, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria pool 15 in the step 3) into the aerobic denitrifying bacteria pool 12, controlling the ammonia nitrogen concentration of the overflowing liquid flowing into the aerobic denitrifying bacteria pool 12 to be less than 2mg/L, collecting the overflowing liquid, adding NaAC and HAc to form a composite carbon source according to the 15.2-fold conversion of the total nitrogen concentration in the nitrifying bacteria nutrient solution when the collected overflowing liquid reaches 72% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid with the volume of 10% of the overflowing liquid, starting aeration for culturing, after starting aeration culture of the aerobic denitrifying bacteria pool 12, communicating a third water pump 43 and a fourth water pump 44, starting the fourth water pump 44 simultaneously when the third water pump 43 runs, wherein the third water pump 43 and the fourth water pump 44 have the same model and the same size, and the feed liquid replenishing speed are the same;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank 12 in the step 4) into the flocculation sedimentation tank 13, triggering the first water pump 41 and the second water pump 42 to respectively add PAC and PAM when the liquid level of the overflowing liquid in the flocculation sedimentation tank 13 rises to the liquid level electrode 135, fully mixing under the action of aeration, selecting a proper water outlet through manual observation after the aeration is finished to discharge supernatant, discharging the residual bacteria sediment through a water discharge pipe 131 at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain the aerobic denitrifying bacteria product.
In the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
Figure BDA0002726437650000191
Figure BDA0002726437650000201
in the step 1), the microcarrier is 100-500-mesh double-flying powder.
In the step 1), the third water pump 43 is controlled by controlling the electric cabinet 3, and when the pH value in the first nitrifying bacteria tank 14 is reduced to below 7.0, the third water pump 43 is started to feed the nitrifying bacteria for feeding, so as to maintain the pH value in the first nitrifying bacteria tank 14 to be 8.2.
The nitrifying bacteria feed comprises the following raw materials in concentration:
Figure BDA0002726437650000202
in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool 14 comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
In the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 1.2, and the mass concentration of the composite carbon source is 20 times of the total nitrogen concentration of the nitrifying bacteria feed liquid.
In the step 5), the concentration of adding PAM is 0.2%, and the concentration of adding PAC is 5%.
In the step 5), the flow rate of PAC when PAC is added is 10mL/min, and the flow rate of PAM when PAM is added is 4 mL/min.
In said step 5), PAC was continuously added at 10mL/min for 10min, while PAM10s was continuously added at 4 mL/min.
Other technical features of this embodiment are the same as those of embodiment 1, and will not be described again.
The above-described embodiments are preferred implementations of the present invention, and the present invention may be implemented in other ways without departing from the spirit of the present invention.

Claims (6)

1. A method for synchronously culturing composite nitrifying bacteria and aerobic denitrifying bacteria is characterized in that: the method comprises the following steps:
1) preparing nitrobacteria nutrient solution, adding the nitrobacteria nutrient solution into a first nitrobacteria pool and a second nitrobacteria pool respectively, adding microcarriers into the first nitrobacteria pool and the second nitrobacteria pool, inoculating nitrobacteria strains according to 5-10% of the volume of the nutrient solution, starting aeration and stirring, and culturing for 5-10 d;
2) detecting the nitrification activity of the thalli in the first nitrifying bacteria pool, and when the nitrification activity of the thalli reaches 30-40mg (NH)3After N)/(L.h), discharging 50-70% of the bacterial liquid in the pool to prepare a product, and simultaneously supplementing nutrient solution and microcarrier with the same concentration as the nutrient solution in the step 1) according to the volume of the discharged bacterial liquid to continue culturing;
3) overflowing the nitrifying bacteria liquid cultured in the first nitrifying bacteria pool into a second nitrifying bacteria pool, and repeating the operation in the step 2) to continuously obtain nitrifying bacteria liquid;
4) overflowing the nitrifying bacteria liquid cultured in the second nitrifying bacteria tank in the step 3) into an aerobic denitrifying bacteria tank, controlling the ammonia nitrogen concentration of overflow liquid flowing into the aerobic denitrifying bacteria tank to be less than 2mg/L, collecting the overflow liquid, adding NaAC and HAc according to the conversion of 14.8-15.2 times of the total nitrogen concentration in the nitrifying bacteria nutrient solution to form a composite carbon source when the collected overflow liquid reaches 68-72% of the effective volume, then inoculating aerobic denitrifying bacteria seed liquid of which the volume is 2-10% of the volume of the overflow liquid, and starting aeration for culturing;
5) overflowing the aerobic denitrifying bacteria liquid cultured in the aerobic denitrifying bacteria tank in the step 4) into a flocculation sedimentation tank, adding PAC and PAM respectively after the liquid level of the overflowing liquid in the flocculation sedimentation tank rises to a certain height, fully mixing under the action of aeration, discharging supernatant through selecting a proper water outlet by manual observation after the aeration is finished, discharging the rest thallus precipitate through a thallus discharge outlet at the bottom of the tank, and finally obtaining an aerobic denitrifying bacteria product through a preparation;
6) repeating the operation in the step 5) to continuously obtain an aerobic denitrifying bacteria product;
in the step 1), adding nitrobacteria for supplementing when the pH value in the first nitrobacteria pool is reduced to below 7.0, and maintaining the pH value in the first nitrobacteria pool to be 7.5-8.2;
in the step 5), the mass concentration of PAM is 0.05-0.2%, and the mass concentration of PAC is 2-5%;
in the step 1), the nitrifying bacteria nutrient solution comprises the following raw materials in concentration:
NH4Cl 0.1-1g/L
Na2CO3 0.1-1g/L
(NH4)2SO4 0.1-1g/L
K2HPO4 0.05-0.5g/L
FeSO4·7H2O 2-10mg/L
MgSO4·7H2O 5-50mg/L
MnCl2·4H2O 5-30mg/L
CuSO4·5H2O 0.5-2mg/L；
the nitrifying bacteria feed comprises the following raw materials in concentration:
Na2CO3 3-15g/L
K2HPO4 0.05-0.5g/L
NH4HCO3 5-20g/L
FeSO4·7H2O 2-10mg/L
MgSO4·7H2O 5-50mg/L
MnCl2·4H2O 5-30mg/L
CuSO4·5H2O 0.5-2mg/L。
2. the method for synchronously culturing the composite nitrifying bacteria and the aerobic denitrifying bacteria according to claim 1, wherein the culture medium comprises the following components: in the step 1), the microcarrier is at least one of 100-mesh 500-mesh zeolite powder, perlite, diatomite, volcanic rock and double-flying powder.
3. The method for synchronously culturing the composite nitrifying bacteria and the aerobic denitrifying bacteria according to claim 1, wherein the culture medium comprises the following components: in the step 2), the method for detecting the nitrification activity of the bacteria in the first nitrifying bacteria pool comprises the following steps: taking 100mL of nitrifying bacteria liquid for centrifugation, mixing the centrifuged precipitate with 100mL of nitrifying bacteria nutrient solution, putting the mixture into a 500mL triangular flask, putting the flask into a constant-temperature shaking table with the temperature of 30 ℃ and the rotating speed of 180rpm for culture, respectively taking samples for 1h, 2h, 3h, 4h and 5h for culture to detect the ammonia nitrogen concentration of the nitrifying bacteria nutrient solution, taking the ammonia nitrogen concentration of the 5 time points as a vertical coordinate, taking time as a horizontal coordinate for straight line fitting, and obtaining the absolute value of the slope of the straight line, namely the nitrification activity value.
4. The method for synchronously culturing the composite nitrifying bacteria and the aerobic denitrifying bacteria according to claim 1, wherein the culture medium comprises the following components: in the step 4), the composite carbon source is prepared by mixing NaAC and HAc according to a molar ratio of 1: 0.8-1.2.
5. The method for synchronously culturing nitrifying bacteria and aerobic denitrifying bacteria according to any one of claims 1 to 4, wherein: an apparatus for implementing the method of synchronously culturing nitrifying bacteria and aerobic denitrifying bacteria according to any one of claims 1 to 4, comprising a biochemical reaction zone and a feed-batch zone communicated with the biochemical reaction zone, wherein the biochemical reaction zone comprises an air compression device, an aerobic denitrifying bacteria tank, a flocculation sedimentation tank, a first nitrifying bacteria tank, a second nitrifying bacteria tank and a plurality of aeration pipes, the second nitrifying bacteria tank is arranged at one side of the first nitrifying bacteria tank and communicated with the upper end of the first nitrifying bacteria tank, the aerobic denitrifying bacteria tank is arranged at one side of the second nitrifying bacteria tank and communicated with the second nitrifying bacteria tank, the flocculation sedimentation tank is arranged at one side of the aerobic denitrifying bacteria tank and communicated with the aerobic denitrifying bacteria tank, and the aeration pipes are all arranged at the bottoms of the first nitrifying bacteria tank, the second nitrifying bacteria tank and the aerobic denitrifying bacteria tank, and the aeration pipes in the flocculation sedimentation tank are inserted into the flocculation sedimentation tank from top to bottom, each aeration pipe is communicated with the air compression device, and the air compression device is arranged on the other side of the nitrifying bacteria tank.
6. The method for synchronously culturing the composite nitrifying bacteria and the aerobic denitrifying bacteria according to claim 5, wherein the culture medium comprises the following components: the feed supplement district is including containing nitrobacteria feed supplement jar, good oxygen denitrification fungus carbon source jar, PAM holding vessel and PAC holding vessel, contain nitrobacteria feed supplement jar with first nitrobacteria pond intercommunication, good oxygen denitrification fungus carbon source jar with second nitrobacteria pond intercommunication, PAM holding vessel with good oxygen denitrification fungus pond intercommunication, the PAC holding vessel with the flocculation and precipitation pond intercommunication, the device is equipped with the control electricity cabinet, agitating unit all with the control electricity cabinet is connected, the control electricity cabinet is located one side of flocculation and precipitation pond.
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