CN112210505A - Preparation method of efficient composite probiotics for poultry intestinal tracts - Google Patents
Preparation method of efficient composite probiotics for poultry intestinal tracts Download PDFInfo
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- CN112210505A CN112210505A CN201910627094.8A CN201910627094A CN112210505A CN 112210505 A CN112210505 A CN 112210505A CN 201910627094 A CN201910627094 A CN 201910627094A CN 112210505 A CN112210505 A CN 112210505A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a preparation method of a Baijialinan high-efficiency composite probiotic for poultry intestinal tracts, which comprises the steps of respectively culturing lactobacillus acidophilus, bacillus subtilis, enterococcus and bacillus belgii in a culture base to obtain lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus belgii liquid; and mixing the lactobacillus acidophilus liquid, the bacillus subtilis liquid, the enterococcus liquid and the bacillus belief to obtain a mixed liquid. According to the preparation method of the Baijialinning efficient composite probiotics for poultry intestinal tracts, disclosed by the invention, the composite probiotics is applied to poultry feed, has the effects of helping digestion, softening excrement and the like, can enhance the immunity of poultry organisms, has a good prevention and treatment effect on diseases caused by pathogenic bacteria in the poultry intestinal tracts, and can prevent and treat diseases of poultry digestive tracts, intestinal tracts and respiratory tracts, and promote the poultry to grow healthily and quickly.
Description
Technical Field
The invention belongs to the field of biology, and particularly relates to a preparation method of a Baijialinning efficient composite probiotic for poultry intestinal tracts.
Background
With the current increasing intensification degree of the breeding industry, the development trend of poultry diseases is more and more complex and changeable; colibacillosis of poultry is a disease caused by pathogenic colibacillosis, which is widely distributed in nature, such as air, dust, padding, feed, drinking water, poultry body surfaces, hatcheries and the like in poultry houses, the poultry diseases are more, which can cause low product quality, meanwhile, the residual poultry can cause food safety problems to threaten human health, the poultry excrement polluted egg is the most main infection source for poultry infection, the poultry embryo infection can be caused by the invasion of the polluted egg colibacillosis through egg shells and shell membranes, the poultry embryo infection can also be caused through digestive tract and respiratory tract infection, the poor breeding management and the poor sanitation condition can cause the disease, the poultry colibacillosis is easy to be infected with the mildew body disease, and other infectious diseases such as new castle disease, bird flu confusion, poultry colibacillosis, poultry infection, and the like, Infectious bronchitis, pasteurellosis and the like are difficult to treat, become important infectious diseases which harm the breeding industry and bring huge economic loss.
The composite probiotics can promote the growth of animals and inhibit diseases, and the composite probiotics can deodorize and improve the feeding environment; can also promote animal health, effectively control and prevent bacteria and parasites, intestinal tract system diseases and respiratory system diseases; meanwhile, the compound probiotics has good prevention and treatment effects on diseases caused by pathogenic bacteria in poultry intestinal tracts, so that the healthy growth of animals is promoted, and the quality of green poultry and livestock products is improved.
Disclosure of Invention
The invention mainly aims to provide a preparation method of a Baijialining high-efficiency composite probiotic for poultry intestinal tracts, which can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the technical scheme that:
the preparation method of the Baikaliin high-efficiency compound probiotics for the poultry intestinal tracts comprises the following steps:
s1, respectively culturing lactobacillus acidophilus, bacillus subtilis, enterococcus and bacillus beilis in a culture base to obtain lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus beilis liquid;
s2, adding the obtained lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus belief bacteria liquid into an LB liquid culture medium for amplification culture and adjustment, and respectively obtaining lactobacillus acidophilus liquid with the concentration of 0.5 multiplied by 108-1.0 multiplied by 108CFU/ml, bacillus subtilis liquid with the concentration of 3.5-6.6 multiplied by 1012CFU/ml, enterococcus liquid with the concentration of 0.5-1.0 multiplied by 108CFU/ml, bacillus subtilis liquid with the concentration of 5.1-7.6 multiplied by 1012CFU/ml and bacillus belief bacteria liquid with the concentration of 6.5-8.3 multiplied by 1013 CFU/ml;
s3, mixing the lactobacillus acidophilus liquid, the bacillus subtilis liquid, the enterococcus liquid and the bacillus beiLeisi liquid which are subjected to amplification culture to obtain mixed liquid;
s4, mixing soybean meal powder, corn protein powder, whey powder and water according to a mass ratio of 2: 3: 2:0.5, adding the mixture into a rotary spherical digester, and mixing to obtain a composite carrier;
and S5, cooling the obtained composite carrier to below 35 ℃, and quickly mixing the cooled composite carrier with the obtained mixed bacterial liquid to finally obtain the composite probiotics.
Preferably, the temperature of the culture base is 37 ℃ and the culture time is 24 h.
Preferably, the pressure of the rotary.
Preferably, the compound probiotics is drops, and is suitable for storage at the temperature of 2-8 ℃ or refrigeration storage.
Preferably, the compound probiotics is a drop culture medium, the temperature is 37 ℃, and the rotating speed is 150 r/min.
Compared with the prior art, the invention has the following beneficial effects: the compound probiotics can promote animal growth and inhibit intestinal diseases, and the compound probiotics has deodorizing effect and can improve feeding environment; can also promote the health of poultry, effectively control and prevent bacteria and parasites, intestinal diseases and respiratory diseases; meanwhile, the compound probiotics has good prevention and treatment effects on diseases caused by pathogenic bacteria in the intestinal tracts of the poultry, reduces the probability of poultry diseases, enables the meat and eggs of the poultry to be good in quality, and improves the quality of green poultry products.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1
Respectively culturing 5 parts of lactobacillus acidophilus, 5 parts of bacillus subtilis, 5 parts of enterococcus and 5 parts of bacillus belgii in culture media at the temperature of 37 ℃ for 24 hours to obtain lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus belgii liquid; weighing 20 g of culture medium, putting into 800 ml of water, fully and uniformly mixing, dividing into four parts, sterilizing for 20 minutes, respectively adding 3ml of each of the obtained lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus belief, into four parts of LB liquid, carrying out amplification culture and adjustment under the conditions that the temperature is 37 ℃ and the rotating speed is 150r/min, and respectively obtaining the lactobacillus acidophilus liquid with the concentration of 0.3 x 88-1.0 x 108CFU/ml, the bacillus subtilis liquid with the concentration of 3.5-6.1 x 1012CFU/ml, the enterococcus liquid with the concentration of 0.5-1.0 x 108CFU/ml, the bacillus subtilis liquid with the concentration of 4.1-6.6 x 1012CFU/ml and the bacillus belief with the concentration of 5.6-8.1 x 1013 CFU/ml; then mixing the lactobacillus acidophilus liquid, the bacillus subtilis liquid, the enterococcus liquid and the bacillus belief to obtain a mixed liquid; mixing soybean meal powder, corn protein powder, whey powder and water according to a mass ratio of 2: 3: 2, adding 0.5 part of the mixture into a rotary spherical digester, and mixing the mixture under the pressure of 0.1-0.12MPa and the sterilization time of 20min to obtain a composite carrier; and finally, cooling the obtained composite carrier to below 35 ℃, and quickly mixing the cooled composite carrier with the obtained mixed bacteria liquid to finally obtain the composite probiotics, wherein the obtained composite probiotics are stored in a cold storage.
The detection shows that the composite probiotics are drops, the color is yellowish liquid, the total viable count is more than 1.65 multiplied by 108cfu/g, the composite probiotics milk has light milk fragrance, and each drop of liquid is rich in 17 hundred million active probiotics.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. The preparation method of the Baikaliin high-efficiency compound probiotics for the poultry intestinal tracts is characterized by comprising the following steps of:
s1, respectively culturing lactobacillus acidophilus, bacillus subtilis, enterococcus and bacillus beilis in a culture base to obtain lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus beilis liquid;
s2, adding the obtained lactobacillus acidophilus liquid, bacillus subtilis liquid, enterococcus liquid and bacillus belief bacteria liquid into an LB liquid culture medium for amplification culture and adjustment, and respectively obtaining lactobacillus acidophilus liquid with the concentration of 0.5 multiplied by 108-1.0 multiplied by 108CFU/ml, bacillus subtilis liquid with the concentration of 3.5-6.6 multiplied by 1012CFU/ml, enterococcus liquid with the concentration of 0.5-1.0 multiplied by 108CFU/ml, bacillus subtilis liquid with the concentration of 5.1-7.6 multiplied by 1012CFU/ml and bacillus belief bacteria liquid with the concentration of 6.5-8.3 multiplied by 1013 CFU/ml;
s3, mixing the lactobacillus acidophilus liquid, the bacillus subtilis liquid, the enterococcus liquid and the bacillus beiLeisi liquid which are subjected to amplification culture to obtain mixed liquid;
s4, mixing soybean meal powder, corn protein powder, whey powder and water according to a mass ratio of 2: 3: 2:0.5, adding the mixture into a rotary spherical digester, and mixing to obtain a composite carrier;
and S5, cooling the obtained composite carrier to below 35 ℃, and quickly mixing the cooled composite carrier with the obtained mixed bacterial liquid to finally obtain the composite probiotics.
2. The preparation method of the highly effective composite probiotics for poultry intestinal tract Baikaliin according to claim 1, is characterized by comprising the following steps: the temperature of the culture base is 37 ℃, and the culture time is 24 h.
3. The preparation method of the highly effective composite probiotics for poultry intestinal tract Baikaliin according to claim 1, is characterized by comprising the following steps: the rotary.
4. The preparation method of the highly effective composite probiotics for poultry intestinal tract Baikaliin according to claim 1, is characterized by comprising the following steps: the compound probiotics is drop, and is suitable for preservation at 2-8 ℃ or refrigeration preservation.
5. The preparation process of the preparation method of the highly effective composite probiotics for poultry intestinal tract according to claim 1, is characterized by comprising the following steps: the culture medium is at 37 deg.C and rotation speed of 150 r/min.
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| CN201910627094.8A CN112210505A (en) | 2019-07-12 | 2019-07-12 | Preparation method of efficient composite probiotics for poultry intestinal tracts |
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| CN201910627094.8A CN112210505A (en) | 2019-07-12 | 2019-07-12 | Preparation method of efficient composite probiotics for poultry intestinal tracts |
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| CN112210505A true CN112210505A (en) | 2021-01-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568601A (en) * | 2022-03-22 | 2022-06-03 | 安徽农业大学 | Probiotic agent for meat duck feed and preparation method thereof |
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| CN104212735A (en) * | 2013-09-06 | 2014-12-17 | 郑州后羿制药有限公司 | Poultry composite probiotic preparation and preparation method thereof |
| CN107549456A (en) * | 2017-10-13 | 2018-01-09 | 安徽农业大学 | A kind of compound probiotic agent for improving goose function of intestinal canal and preparation method thereof |
| CN107629981A (en) * | 2017-10-13 | 2018-01-26 | 安徽农业大学 | A kind of compound probiotic agent for improving chick function of intestinal canal and preparation method thereof |
| CN107746818A (en) * | 2017-10-13 | 2018-03-02 | 安徽农业大学 | A kind of compound probiotic agent for improving intestines function of piglings and preparation method thereof |
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2019
- 2019-07-12 CN CN201910627094.8A patent/CN112210505A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212735A (en) * | 2013-09-06 | 2014-12-17 | 郑州后羿制药有限公司 | Poultry composite probiotic preparation and preparation method thereof |
| CN107549456A (en) * | 2017-10-13 | 2018-01-09 | 安徽农业大学 | A kind of compound probiotic agent for improving goose function of intestinal canal and preparation method thereof |
| CN107629981A (en) * | 2017-10-13 | 2018-01-26 | 安徽农业大学 | A kind of compound probiotic agent for improving chick function of intestinal canal and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114568601A (en) * | 2022-03-22 | 2022-06-03 | 安徽农业大学 | Probiotic agent for meat duck feed and preparation method thereof |
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