CN112210484A - Picking needle assembly and high-throughput cloning and selecting equipment - Google Patents
Picking needle assembly and high-throughput cloning and selecting equipment Download PDFInfo
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- CN112210484A CN112210484A CN202011260541.XA CN202011260541A CN112210484A CN 112210484 A CN112210484 A CN 112210484A CN 202011260541 A CN202011260541 A CN 202011260541A CN 112210484 A CN112210484 A CN 112210484A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a picking needle assembly and high-flux cloning and selecting equipment, which comprise a picking needle plate and a plurality of driving cavities arranged on the picking needle plate, wherein a picking needle is arranged in each driving cavity in a sliding mode, the working end of the picking needle can extend out of the first end of each driving cavity, the second end of each driving cavity is provided with a driving port communicated with the corresponding driving cavity, and the picking needle can be driven to move towards the first end close to the corresponding driving cavity through the driving port. When the picking needle assembly is adopted, driving gas is introduced into the driving cavity through the driving port or the driving piece pushes the picking needle to be close to the first end close to the driving cavity, and the working end of the picking needle can extend out of the first end of the driving cavity, so that the picking needle assembly finishes the picking operation. Because the number of the driving chambers is multiple, the operation of selecting multiple clones can be completed simultaneously, so that the selection efficiency of high-flux clones is improved, and the labor intensity is reduced.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to a picking needle assembly and high-throughput clone picking equipment.
Background
The selection of clones is an important step in high-throughput cloning. At present, the selection of clones by using a pipettor and a gun head to select manually has no great influence on the selection of small batches of clones, but when the selection of high-throughput clones is carried out, the working efficiency is too low, and the labor intensity is high.
Therefore, how to improve the efficiency of selecting high-throughput clones and reduce labor intensity is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The invention aims to provide a picking needle assembly and high-throughput clone selection equipment, so that the selection efficiency of high-throughput clones is improved, and the labor intensity is reduced.
In order to achieve the above purpose, the present invention provides a picker assembly, including a picker plate and a plurality of driving chambers disposed on the picker plate, wherein one picker is slidably disposed in each driving chamber, a working end of the picker can extend out of a first end of the driving chamber, a second end of the driving chamber is provided with a driving port communicated with the driving chamber, and the picker can be driven to move towards the first end close to the driving chamber through the driving port.
In one embodiment of the present invention, the picking needle includes a sliding end and a working end connected to the sliding end, wherein the sliding end is slidably engaged with the driving chamber, and the working end can extend out of the first end of the driving chamber.
In one embodiment of the present invention, the sliding end and the working end are an integrated structure or a split structure.
In one embodiment of the present invention, a first sealing member is disposed on a circumferential surface of the sliding end, and the first sealing member is closely fitted to an inner wall of the driving chamber.
In one embodiment of the invention, the needle picking device further comprises a sealing plate arranged on part or all of the end face of the needle picking plate, the sealing plate is used for sealing one or more driving chambers, a needle picking port is arranged at a position of the sealing plate corresponding to the driving chambers, and the working end can extend out of the first end of the driving chamber through the needle picking port.
In one embodiment of the present invention, the sealing plate further comprises an elastic member disposed at the working end and abutting against the sliding end and the sealing plate.
In one embodiment of the invention, the driving chamber is surrounded by a hole arranged on the picker plate.
In one embodiment of the invention, the driving chamber is enclosed by a cylindrical shell arranged on the picker plate.
In one embodiment of the present invention, a plurality of the driving chambers are arranged in a row.
The invention also discloses high-throughput clone selection equipment which comprises a switching component and the picking needle component, wherein the switching component drives the picking needle of the picking needle component to move towards the first end of the driving cavity close to the picking needle component through the driving port of the picking needle component.
In one embodiment of the present invention, the switching assembly includes a valve set mechanism having a plurality of air outlets, each air outlet communicating with the driving port of the picking needle assembly.
In one embodiment of the present invention, the valve train mechanism includes a plurality of three-way valves fixed together, each of the three-way valves having one of the air outlets.
In one embodiment of the present invention, the three-way valve is an electromagnetic three-way valve.
In one embodiment of the present invention, the controller controls the conduction and non-conduction of the corresponding three-way valve.
In one embodiment of the invention, each air outlet is connected with the driving port through an air cylinder mechanism.
In one embodiment of the invention, the cylinder mechanism comprises a plurality of cylinders, an inlet of each cylinder is communicated with one of the air outlets, and a piston rod of each cylinder is in contact with the picking needle of the picking needle assembly through the driving port.
In one embodiment of the invention, the inlet of each cylinder is communicated with one air outlet through an air pipe.
In one embodiment of the present invention, each of the air outlets is communicated with the air pipe through a joint.
In one embodiment of the invention, each air cylinder is arranged on the picking needle assembly through a fastening nut.
In one embodiment of the invention, the picking needle assembly is provided with a fixing plate, the fixing plate is provided with a plurality of mounting holes, and each air cylinder is fixed on the mounting holes through fastening nuts.
In one embodiment of the invention, a position avoiding groove is formed in the end face, close to the picking needle assembly, of the fixing plate.
The invention has the following beneficial effects:
when the picking needle assembly is adopted, driving gas is introduced into the driving cavity through the driving port or the driving piece pushes the picking needle to be close to the first end close to the driving cavity, and the working end of the picking needle can extend out of the first end of the driving cavity, so that the picking needle assembly finishes the picking operation. Because the number of the driving chambers is multiple, the operation of selecting multiple clones can be completed simultaneously, so that the selection efficiency of high-flux clones is improved, and the labor intensity is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic perspective view of a picking needle assembly according to the present invention;
FIG. 2 is a cross-sectional view of a pick needle assembly according to the present invention in a first state;
FIG. 3 is a schematic cross-sectional view of a pick needle assembly according to the present invention in a second state;
FIG. 4 is a cross-sectional view of another pick needle assembly provided in accordance with the present invention in a first state;
FIG. 5 is a schematic cross-sectional view of another pick needle assembly provided in accordance with the present invention in a second state;
FIG. 6 is a schematic perspective view of another pick-up needle assembly according to the present invention;
FIG. 7 is a cross-sectional view of another pick needle assembly provided in accordance with the present invention in a first state;
FIG. 8 is a cross-sectional view of another pick needle assembly provided in accordance with the present invention in a second state;
FIG. 9 is a schematic perspective view of a high throughput clone selection device according to the present invention;
fig. 10 is a schematic perspective view of a valve train mechanism according to the present invention;
fig. 11 is a schematic perspective view of a cylinder mechanism according to the present invention;
FIG. 12 is a schematic view of a connection structure of the picking needle assembly and the cylinder mechanism according to the present invention;
FIG. 13 is a schematic cross-sectional view of a pick-up needle assembly in a first state coupled to a cylinder mechanism according to the present invention;
FIG. 14 is a schematic cross-sectional view of a pick-up needle assembly in a second state connected to a cylinder mechanism according to the present invention;
FIG. 15 is a cross-sectional view of a further pick pin assembly of the present invention connected to a cylinder mechanism in a first state;
FIG. 16 is a schematic cross-sectional view of a further pick pin assembly of the present invention in a second state connected to a cylinder mechanism;
FIG. 17 is a schematic perspective view of another pick-up needle assembly and cylinder mechanism according to the present invention;
FIG. 18 is a cross-sectional view of a further pick pin assembly provided in accordance with the present invention in a first state in connection with a cylinder mechanism;
FIG. 19 is a cross-sectional view of another pick pin assembly of the present invention in a second state in connection with a cylinder mechanism;
in the figure: 100 is a picking needle assembly, 200 is a valve group mechanism, 300 is an air cylinder mechanism, 400 is an air pipe, 101 is a picking needle plate, 102 is a driving chamber, 103 is a picking needle, 104 is an elastic piece, 105 is a sealing plate, 106 is a fixing plate, 1021 is a driving port, 201 is a three-way valve, 202 is a joint, 203 is a mounting plate, 301 is a piston rod, 302 is a fastening nut, 1031 is a sliding end, 1032 is a working end, 1061 is a mounting hole, and 1062 is a clearance groove.
Detailed Description
The core of the invention is to provide a picking needle assembly and high-throughput clone selection equipment so as to improve the selection efficiency of high-throughput clones and reduce the labor intensity.
In order that those skilled in the art will better understand the disclosure, the invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Referring to fig. 1 to 8, a picker assembly 100 according to an embodiment of the present invention includes a picker plate 101 and a plurality of driving chambers 102 disposed on the picker plate 101, wherein a picker 103 is slidably disposed in each driving chamber 102, a working end 1032 of the picker 103 extends out of a first end of the driving chamber 102, a second end of the driving chamber 102 is disposed with a driving port 1021 communicating with the driving chamber 102, and the picker 103 is driven to move toward the first end of the driving chamber 102 through the driving port 1021.
When the picking needle assembly 100 of the present invention is used, the driving gas is introduced into the driving chamber 102 through the driving port 1021 or the driving member pushes the picking needle 103 to approach the first end of the driving chamber 102, and the working end 1032 of the picking needle 103 can extend out of the first end of the driving chamber 102, so that the picking needle assembly 100 can complete the picking operation. Since the number of the driving chambers 102 is plural, the operation of selecting a plurality of clones can be simultaneously completed, so that the selection efficiency of high-throughput clones is improved, and the labor intensity is reduced.
The driving chamber 102 is a structure for mounting the picker needle 103, and the driving chamber 102 is defined by a hole provided in the picker plate 101 or a cylindrical case provided in the picker plate 101. The driving chambers 102 are arranged in a regular pattern, in a ring, in an array, etc., although the plurality of driving chambers 102 may be arranged in an irregular pattern.
The picker needle 103 includes a sliding end 1031 and a working end 1032 connected to the sliding end 1031, wherein the sliding end 1031 is slidably engaged with the driving chamber 102, and the working end 1032 can extend out of the driving chamber 102. The sliding end 1031 and the working end 1032 are of an integral structure or a split structure. In order to ensure the sealing of the sliding end 1031 and the driving chamber 102, a second sealing member closely matching with the inner wall of the driving chamber 102 is disposed on the circumferential surface of the sliding end 1031.
The picker assembly 100 further includes a sealing plate 105 disposed on part or all of the end surface of the picker plate 101, the sealing plate 105 being configured to seal one or more of the drive chambers 102, and a picker opening is provided in a portion of the sealing plate 105 corresponding to the drive chamber 102, through which the working end 1032 can protrude out of the drive chamber 102. Wherein each closure plate 105 encloses one drive chamber 102 in figures 4 to 5; in fig. 6-8, a closure plate 105 encloses the plurality of drive chambers 102.
Further, in order to improve the working efficiency, the picker assembly 100 further includes an elastic member 104 provided at the working end 1032 and abutting the sliding end 1031 and the sealing plate 105 or the picker plate 101. When the driving port 1021 of the picking needle assembly 100 is conducted by the gas, the picking needle 103 runs downward in the driving chamber 102 under the action of the driving gas and overcomes the elastic force of the elastic member 104, extends out of the driving chamber 102, and contacts with the liquid in the culture dish to pick out the clone body. When the picking is completed, the driving port 1021 is not ventilated, and the elastic member 104 drives the picking needle 103 to move upwards, so that the working end 1032 of the picking needle 103 is separated from the liquid in the culture dish.
Referring to fig. 9 to 19, the present invention further discloses a high throughput clone selecting apparatus, which includes a switching assembly and the picking needle assembly 100 as described above, wherein the switching assembly drives the picking needle 103 of the picking needle assembly 100 to move towards the first end of the driving chamber 102 close to the picking needle assembly 100 through the driving port 1021 of the picking needle assembly 100.
It should be noted that the switching assembly may provide the driving gas to the driving port 1021 or provide the driving member through the driving port 1021. The switching assembly provides a driving gas or driving member to each of the driving ports 1021, and when the driving gas is provided, the driving gas pushes the picking needle 103 to move in a direction close to the first end of the driving chamber 102 until the working end 1032 of the picking needle 103 protrudes out of the first end of the driving chamber 102, thereby performing a clone picking operation. In the case of the driving member, the driving member pushes the pick-up needle 103 to move in a direction close to the first end of the driving chamber 102 until the working end of the pick-up needle 103 protrudes out of the first end of the driving chamber 102, thereby realizing the work of picking the clone body.
When the driving gas is supplied to the driving port 1021, the switching assembly includes a valve block mechanism 200, and the valve block mechanism 200 has a plurality of gas outlets each communicating with the driving port 1021 of the picker assembly 100. The valve train mechanism 200 may be an integrated valve or may combine several valves together. For example, the valve train mechanism 200 includes a plurality of three-way valves 201 fixed together, each three-way valve 201 having one outlet port.
The three-way valves 201 may be integrated by a mounting plate 203, and the arrangement may be according to the arrangement of the driving ports 1021, for example, when the driving ports 1021 are arranged, the three-way valves 201 may also be arranged in a row. Of course, the three-way valve 201 may be arranged in a regular or irregular manner, such as in a row, in a cylinder, or the like, instead of the arrangement of the driving port 1021.
The three-way valve 201 of the valve train mechanism 200 may be manually controlled or automatically controlled, for example, the three-way valve 201 may be an electromagnetic three-way valve. The conducting state and the non-conducting state of the electromagnetic three-way valve can be controlled according to a timer. Alternatively, the valve train mechanism 200 of the present invention further includes a controller that controls the conduction and non-conduction of the corresponding three-way valve 201. The controller performs control according to a pre-stored rule, for example, the controller controls the three-way valve 201 to be sequentially conducted in sequence; or the three-way valve 201 is controlled to be switched on according to the interval sequence, so that the selection operation is performed according to a certain rule.
When the driving member is supplied to the driving port 1021, each air outlet is connected to the driving port 1021 through the cylinder mechanism 300 in the embodiment of the present invention. In this embodiment, the piston rod 301 of the air cylinder pushes the picking needle 103 to slide in the driving chamber 102 through the driving port 1021. The cylinder mechanism 300 includes a plurality of cylinders, each cylinder having an inlet connected to an outlet, and a piston rod 301 of each cylinder contacting the picker needle 103 of the picker needle assembly 100 through a driving port 1021.
The cylinders are directly or indirectly communicated with the air outlets of the three-way valve 201, and when the cylinders are indirectly communicated, the inlet of each cylinder is communicated with one air outlet through the air pipe 400. The air tubes 400 may be provided separately, or the air tubes 400 may be connected together through an integrated member.
Further, each air outlet is communicated with the air pipe 400 through the joint 202 for connection convenience. The joint 202 is a quick joint 202, one end of the air tube 400 is connected to the cylinder, and the other end of the air tube 400 is connected to the joint 202.
Each air cylinder is provided on the picker assembly 100 through a fastening nut 302, and the fastening nut 302 is directly or indirectly fixed to the picker assembly 100. When the indirect fixing is performed, the picker assembly 100 is provided with a fixing plate 106, a plurality of mounting holes 1061 are formed in the fixing plate 106, and each cylinder is fixed to the mounting hole 1061 by a fastening nut 302.
In order to reduce the volume of the apparatus, a clearance groove 1062 is provided on the end surface of the fixing plate 106 adjacent to the picker pin assembly 100, or a clearance groove 1062 is also provided on the part of the picker plate 101 adjacent to the fixing plate 106, and the fastening nut 302 of the fixing cylinder can be received through the clearance groove 1062.
The high throughput clone selection device provided by the present invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (21)
1. The utility model provides a picking needle subassembly which characterized in that, is in including the picking plate with set up a plurality of drive cavities on the picking plate, every slidable is provided with one in the drive cavity picking needle, just the work end of picking needle can stretch out the first end of drive cavity, the second end of drive cavity be provided with one with the drive mouth of drive cavity intercommunication, through the drive mouth can drive picking needle is to being close to the first end of drive cavity removes.
2. The needle assembly of claim 1, wherein the needle comprises a sliding end and a working end connected to the sliding end, wherein the sliding end is slidably engaged with the driving chamber and the working end is extendable beyond the first end of the driving chamber.
3. The picker assembly of claim 2, wherein the sliding end and the working end are of a one-piece structure or a split structure.
4. The picker assembly according to claim 3, wherein the sliding end is provided at a circumferential surface thereof with a first sealing member closely fitted to an inner wall of the driving chamber.
5. The picker assembly according to claim 4, further comprising a sealing plate provided on part or all of an end surface of the picker plate, the sealing plate being adapted to seal one or more of the drive chambers, and a picker port being provided on the sealing plate at a position corresponding to the drive chamber, the working end being extendable out of the first end of the drive chamber through the picker port.
6. The picker assembly according to claim 5, further comprising a resilient member disposed at the working end and abutting the sliding end and the sealing plate.
7. The picker assembly according to any one of claims 1 to 6, wherein the drive chamber is bounded by an aperture provided in the picker plate.
8. The picker assembly according to any one of claims 1 to 6, wherein the drive chamber is enclosed by a cylindrical housing provided on the picker plate.
9. The needle picking assembly as claimed in any of claims 1 to 6, wherein a plurality of the drive chambers are arranged in a row.
10. A high throughput clone picking apparatus comprising a switching assembly and the picking needle assembly of any one of claims 1 to 9, said switching assembly driving the picking needle of said picking needle assembly to move towards the first end of the driving chamber of said picking needle assembly through the driving port of said picking needle assembly.
11. The high throughput clone picking apparatus of claim 10, wherein said switching assembly includes a valve block mechanism having a plurality of air outlets, each air outlet communicating with a drive port of said picker pin assembly.
12. The high throughput clone picking apparatus of claim 11, wherein said valve block mechanism includes a plurality of three-way valves secured together, each of said three-way valves having one of said air outlets.
13. The high throughput clone selection device of claim 11, wherein said three-way valve is an electromagnetic three-way valve.
14. The high throughput clone selection device of claim 13, further comprising a controller that controls the conduction and non-conduction of said respective three-way valves.
15. The high throughput clone picking apparatus of claim 11, wherein each of said air outlets is connected to said drive port by an air cylinder mechanism.
16. The high throughput clone picking apparatus of claim 11, wherein said cylinder mechanism includes a plurality of cylinders, an inlet of each of said cylinders being in communication with said one of said outlet ports, a piston rod of each of said cylinders being in contact with a picker needle of said picker needle assembly through said drive port.
17. The high throughput clone picking apparatus of claim 16, wherein the inlet of each of said air cylinders is in communication with one of said air outlets via an air tube.
18. The high throughput clone selection device of claim 17, wherein each of said air vents is in communication with said air tube via a connector.
19. The high throughput clone picking apparatus of claim 16, wherein each of said air cylinders is disposed on said picker pin assembly by a fastening nut.
20. The high throughput clone selection apparatus of claim 16, wherein said picking pin assembly is provided with a fixing plate having a plurality of mounting holes formed therein, each cylinder being fixed to said mounting hole by a fastening nut.
21. The high throughput clone selection device of claim 20, wherein said fixing plate is provided with a position-avoiding groove on an end surface thereof adjacent to said picking pin assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011260541.XA CN112210484A (en) | 2020-11-12 | 2020-11-12 | Picking needle assembly and high-throughput cloning and selecting equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011260541.XA CN112210484A (en) | 2020-11-12 | 2020-11-12 | Picking needle assembly and high-throughput cloning and selecting equipment |
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| CN112210484A true CN112210484A (en) | 2021-01-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011260541.XA Pending CN112210484A (en) | 2020-11-12 | 2020-11-12 | Picking needle assembly and high-throughput cloning and selecting equipment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113667588A (en) * | 2021-07-26 | 2021-11-19 | 中国科学院深圳先进技术研究院 | Picking needle automatic reset mechanism and automatic bacterial colony selecting instrument |
| WO2022257114A1 (en) * | 2021-06-11 | 2022-12-15 | 中国科学院深圳先进技术研究院 | Picking needle automatic reset mechanism, clone picking workstation and clone picking method |
-
2020
- 2020-11-12 CN CN202011260541.XA patent/CN112210484A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022257114A1 (en) * | 2021-06-11 | 2022-12-15 | 中国科学院深圳先进技术研究院 | Picking needle automatic reset mechanism, clone picking workstation and clone picking method |
| CN113667588A (en) * | 2021-07-26 | 2021-11-19 | 中国科学院深圳先进技术研究院 | Picking needle automatic reset mechanism and automatic bacterial colony selecting instrument |
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