CN112209883B - Fluorescein dye specifically combined with RNA and application thereof - Google Patents

Fluorescein dye specifically combined with RNA and application thereof Download PDF

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CN112209883B
CN112209883B CN202010997656.0A CN202010997656A CN112209883B CN 112209883 B CN112209883 B CN 112209883B CN 202010997656 A CN202010997656 A CN 202010997656A CN 112209883 B CN112209883 B CN 112209883B
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rna
pvx
ksk
dfhbi
dna
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CN112209883A (en
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洪益国
俞志明
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Hangzhou Normal University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/96—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1007—Non-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1044—Heterocyclic compounds characterised by ligands containing two nitrogen atoms as heteroatoms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Abstract

A fluorescein dye specifically combined with RNA and application thereof, belonging to the technical field of biology. The application includes a fluorescein dye DFHBI that specifically binds to RNA. The DFHBI is used for specifically combining with plant RNA to realize the application of the plant RNA fluorescent dye. The fluorescent dye DFHBI is not combined with DNA and is specifically combined with RNA, so that the dyed RNA emits green fluorescence; but not staining DNA, no DNA fluorescence. The finding is that DFHBI binds RNA with affinity and fluoresces in ultraviolet light in the long wavelength band (360 nm), and thus can be used as an indicator for determining the size of RNA molecules and analyzing the content of RNA. The discovery can make the characteristics of DFHBI more clear, and provides a theoretical and application basis for further experimental research.

Description

Fluorescein dye specifically combined with RNA and application thereof
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a dye capable of fluorescing after being combined with RNA (but not DNA), wherein the dye can be used as a dye specifically combined with RNA under the irradiation of a long-wave-band ultraviolet lamp, and an application thereof.
Background
RNA visualization can be achieved by using molecular signaling Markers (MBs), RNA binding marker proteins (RBLPs), and RNA aptamer-based methods. Molecular signaling Markers (MBs) relate to an affinity probe that is perfectly complementary to a target RNA in homogeneous solution. RNA binding marker proteins (RBLPs), such as MS2, PUM-HD, hnRNPA1, lambda N22, Cas9, Cas13a, etc., can be used to detect target RNA after binding to specific RNA sequences. Unlike MB and RBLP methods for RNA detection, the RNA aptamer, Spinach green (Spinach, also known as 24-2 or 24-2 min) and its derivative Spinach2, can mimic the target RNA to the same extent as Green Fluorescent Protein (GFP) for RNA visualization. These RNA aptamers, when bound to the fluorescein molecule DFHBI (3, 5-difluoro-4-hydroxybenzylideneimidazolidinone), form an intramolecular G quadruplex and emit green fluorescence.
Dyes commonly used for RNA detection are mainly ethidium bromide, SYBR Green, etc., but these dyes have no specificity for DNA and RNA staining and are generally unable to distinguish DNA from RNA in nucleic acids.
Disclosure of Invention
In view of the problems in the prior art, the present invention is directed to a fluorescent dye specifically binding to RNA and a technical solution for using the same.
The fluorescein dye specifically combined with RNA is characterized in that the fluorescein is DFHBI, and the molecular formula is as follows: c 12 H 10 F 2 N 2 O 2 The molecular weight is: 252.22, the structural formula is shown below:
Figure RE-DEST_PATH_IMAGE001
。
the fluorescein dye specifically combined with the RNA is characterized in that the fluorescein dye can be combined with various RNAs, the RNA fluorescence can be observed under the irradiation of long-wave band UV, and the mixture with the RNA generates the fluorescence similar to GFP under the excitation of blue light.
The fluorescein dye specifically combined with RNA is characterized in that the long-wave band UV wavelength is 365 nm.
The DFHBI is used for specifically combining with plant RNA to realize the application of the plant RNA fluorescent dye, and the molecular formula of the DFHBI is as follows: c 12 H 10 F 2 N 2 O 2 The molecular weight is: 252.22, the structural formula is shown below:
Figure 891822DEST_PATH_IMAGE001
。
the application is characterized in that the plant RNA and the DFHBI have the effect of luminescence, and under the excitation of blue light, the RNA containing Spinach, preferably Spinach green RNA can generate stronger green fluorescence similar to GFP, and the plant RNA is various plant RNAs including lysine transport RNA (tRNA) Lys )。
The fluorescent dye DFHBI of the invention does not bind to DNA, specifically binds to RNA, and causes the RNA to fluoresce, while the DNA is not stained. The discovery is that the affinity of DFHBI and RNA is utilized, and fluorescence is emitted by ultraviolet rays in a long wave band (360 nm), so that the fluorescent probe can be used for indicating and determining the size of an RNA molecule and analyzing and determining the content of the RNA, and provides a theoretical and application basis for further experimental research.
Drawings
FIG. 1 is a diagram of DFHBI-specific stained RNA;
FIG. 2 shows RNA staining of DFHBI with and without spinach green;
FIG. 3 is a structural formula diagram of DFHBI.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific embodiments.
Example 1:
after being combined with RNA, DFHBI emits fluorescence under the irradiation of a long-wave band ultraviolet lamp.
After linearization of the PVX (plant RNA Virus) recombinant plasmid vector (Spe)IRestriction enzyme digestion) (in FIG. 1, M1 represents BM15000, M2 represents BM5000 molecular weight marker, lanes 1-2 are PVX/KK, lanes 3 are PVX/KSK, lanes 4 are PVX/AtFT-KSK, lanes 5 are PVX/mATFT-KSK, lanes 6 are PVX/AtTFL 1-KSK), the T7 promoter drives RNA polymerase to transcribe the linearized plasmid into PVX recombinant RNA in vitro, after the transcription reaction in vitro, 5 μ l of sample (RNA is 5 μ g) is added into 1% agarose gel configured by TAE, and electrophoresis is carried out (voltage is: 5V/cm). After electrophoresis is finished, the gel is soaked in 200 mu M DFHBI solution for 15min, a Canon single-lens reflex camera with a UV filter is used for photographing and recording under a long-wave-band ultraviolet lamp (365 nm), then the gel is washed 3 times with RNase-free water, each time is 2min, and after the gel is soaked for 10min by 10 mu g/ml ethidium bromide, photographing and recording under the UV of a long-wave band and a short-wave band respectively.
Wherein, the nucleotide sequence of lysine transport RNA (K) is shown as SEQ ID No.1, the nucleotide sequence of spinach green RNA (S) is shown as SEQ ID No.2, the nucleotide sequence of lysine transport RNA-spinach green RNA-lysine transport RNA (KSK) is shown as SEQ ID No.3, the nucleotide sequence of PVX/KK is shown as SEQ ID No.4, the nucleotide sequence of PVX/AtFT-KSK is shown as SEQ ID No.10, the nucleotide sequence of PVX/mATFT-KSK is shown as SEQ ID No.11, and the nucleotide sequence of PVX/AtTFL1-KSK is shown as SEQ ID No. 12.
Results in visible light after immersion of the electrophoresis gel in DFHBI, fig. 1 a; under the long-wave band UV condition, DFHBI stains RNA, and after affinity binding, the RNA fluoresces; whereas DFHBI did not stain DNA, sample DNA and BM15000 and BM5000 marker DNA, with no fluorescent signal (B in fig. 1); in the presence of EB, DNA (sample DNA as well as BM15000 and BM5000 marker DNA) and RNA fluoresce regardless of short wavelength UV (C in FIG. 1) or long wavelength UV (D in FIG. 1).
Example 2:
DFHBI non-specifically binds to RNA, can bind to green RNA without spinach and green RNA with spinach, and generates green fluorescence; but produces a green fluorescence intensity with spinach green containing RNA.
Based on the PVX vector, PVX/KK, PVX/KSK 1, PVX/KSK 2, PVX/KSK 3, PVX/KSK 4, PVX/KSK 5 vectors (A in FIG. 2) were constructed (K represents lysine transport RNA, S represents Spinach RNA,. sup.1,. sup.2,. sup.3,. sup.4,. sup.5 represents 1 to 5 KSKs linked in series into the PVX vector). Successfully constructed PVX recombinant vector linearization (restriction endonuclease Spe I digestion), RNA polymerase is driven by a T7 promoter to perform in vitro transcription (B in figure 2), after the obtained PVX recombinant viral RNA is mixed with DFHBI (200 mu M), a sample (C in figure 2) with yellow green color can be seen under visible light, and under a light source excited by blue light, green fluorescence similar to GFP can be seen (D in figure 2, 40-second exposure) (E in figure 2, 150-second exposure). The corresponding sample in the figure is shown as F in fig. 2.
Wherein, the nucleotide sequence of PVX/KSK 1 is shown as SEQ ID No.5, the nucleotide sequence of PVX/KSK 2 is shown as SEQ ID No.6, the nucleotide sequence of PVX/KSK 3 is shown as SEQ ID No.7, the nucleotide sequence of PVX/KSK 4 is shown as SEQ ID No.8, and the nucleotide sequence of PVX/KSK 5 is shown as SEQ ID No. 9.
FIG. 3 shows the chemical formula of the DFHBI fluorescent dye.
Sequence listing
<110> university of teachers in Hangzhou
<120> fluorescein dye specifically combined with RNA and application thereof
<160> 12
<170> SIPOSequenceListing 1.0
<210> 1
<211> 73
<212> RNA
<213> lysine transport RNA (tRNALys)
<400> 1
gcccgucuag cucaguuggu agagcgcaag gcucuuaacc uuguggucgu ggguucgagc 60
cccacggugg gcg 73
<210> 2
<211> 80
<212> RNA
<213> spinach Green RNA (spinach)
<400> 2
gacgcgaccg aaauggugaa ggacgggucc agugcuucgg cacuguugag uagaguguga 60
gcuccguaac uggucgcguc 80
<210> 3
<211> 250
<212> RNA
<213> lysine Transporter RNA-Spinach Green RNA-lysine Transporter RNA (tRNALys-Spinach-tRNALys)
<400> 3
accggugccc gucuagcuca guugguagag cgcaaggcuc uuaaccuugu ggucgugggu 60
ucgagcccca cggugggcga agcuugacgc gaccgaaaug gugaaggacg gguccagugc 120
uucggcacug uugaguagag ugugagcucc guaacugguc gcgucgcaug cgcccgucua 180
gcucaguugg uagagcgcaa ggcucuuaac cuuguggucg uggguucgag ccccacggug 240
ggcgcccggg 250
<210> 4
<211> 9876
<212> DNA
<213> PVX/KK(PVX/KK)
<400> 4
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatacgcgtg cccgtctagc tcagttggta 5700
gagcgcaagg ctcttaacct tgtggtcgtg ggttcgagcc ccacggtggg cgcggccggc 5760
ccgtctagct cagttggtag agcgcaaggc tcttaacctt gtggtcgtgg gttcgagccc 5820
cacggtgggc gtccggatga tatcgtcgac cgccgatgaa cggttaagtt tccattgata 5880
ctcgaaagat gtcagcacca gctagcacaa cacagcccat agggtcaact acctcaacta 5940
ccacaaaaac tgcaggcgca actcctgcca cagcttcagg cctgttcacc atcccggatg 6000
gggatttctt tagtacagcc cgtgccatag tagccagcaa tgctgtcgca acaaatgagg 6060
acctcagcaa gattgaggct atttggaagg acatgaaggt gcccacagac actatggcac 6120
aggctgcttg ggacttagtc agacactgtg ctgatgtagg atcatccgct caaacagaaa 6180
tgatagatac aggtccctat tccaacggca tcagcagagc tagactggca gcagcaatta 6240
aagaggtgtg cacacttagg caattttgca tgaagtatgc tccagtggta tggaactgga 6300
tgttaactaa caacagtcca cctgctaact ggcaagcaca aggtttcaag cctgagcaca 6360
aattcgctgc attcgacttc ttcaatggag tcaccaaccc agctgccatc atgcccaaag 6420
aggggctcat ccggccaccg tctgaagctg aaatgaatgc tgcccaaact gctgcctttg 6480
tgaagattac aaaggccagg gcacaatcca acgactttgc cagcctagat gcagctgtca 6540
ctcgaggtcg tatcactgga acaacaaccg ctgaggctgt tgtcactcta ccaccaccat 6600
aactacgtct acataaccga cgcctacccc agtttcatag tattttctgg tttgattgta 6660
tgaataatat aaataaaaaa aaaaaaaaaa aaaaaaaact agtggtaccg agctcggtac 6720
cccatgcaag cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt 6780
catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6840
gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6900
tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6960
gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7020
actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7080
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7140
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7200
ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7260
aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7320
cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7380
cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7440
aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7500
cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7560
ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7620
ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7680
gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7740
agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7800
acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7860
tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7920
agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7980
gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8040
agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8100
cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8160
ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8220
cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8280
cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8340
ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8400
tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8460
catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8520
gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8580
gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8640
tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8700
catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8760
aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8820
attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8880
aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 8940
aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 9000
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 9060
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 9120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 9180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat 9240
tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 9300
taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 9360
gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 9420
caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 9480
aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 9540
atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 9600
aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 9660
cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa 9720
ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9780
atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9840
aacgacggcc agtgaattgt aatacgactc actata 9876
<210> 5
<211> 10023
<212> DNA
<213> PVX/KSK*1(PVX/KSK*1)
<400> 5
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatacgcgta tattctgccc aaattcgcga 5700
ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg gtcgtgggtt 5760
cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg gtccagtgct 5820
tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc gcccgtctag 5880
ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc cccacggtgg 5940
gcgcccgggc atcaccatca ccatcactac ggccgtgatc cggatgatat cgtcgaccgc 6000
cgatgaacgg ttaagtttcc attgatactc gaaagatgtc agcaccagct agcacaacac 6060
agcccatagg gtcaactacc tcaactacca caaaaactgc aggcgcaact cctgccacag 6120
cttcaggcct gttcaccatc ccggatgggg atttctttag tacagcccgt gccatagtag 6180
ccagcaatgc tgtcgcaaca aatgaggacc tcagcaagat tgaggctatt tggaaggaca 6240
tgaaggtgcc cacagacact atggcacagg ctgcttggga cttagtcaga cactgtgctg 6300
atgtaggatc atccgctcaa acagaaatga tagatacagg tccctattcc aacggcatca 6360
gcagagctag actggcagca gcaattaaag aggtgtgcac acttaggcaa ttttgcatga 6420
agtatgctcc agtggtatgg aactggatgt taactaacaa cagtccacct gctaactggc 6480
aagcacaagg tttcaagcct gagcacaaat tcgctgcatt cgacttcttc aatggagtca 6540
ccaacccagc tgccatcatg cccaaagagg ggctcatccg gccaccgtct gaagctgaaa 6600
tgaatgctgc ccaaactgct gcctttgtga agattacaaa ggccagggca caatccaacg 6660
actttgccag cctagatgca gctgtcactc gaggtcgtat cactggaaca acaaccgctg 6720
aggctgttgt cactctacca ccaccataac tacgtctaca taaccgacgc ctaccccagt 6780
ttcatagtat tttctggttt gattgtatga ataatataaa taaaaaaaaa aaaaaaaaaa 6840
aaaaactagt ggtaccgagc tcggtacccc atgcaagctt ttgttccctt tagtgagggt 6900
taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6960
tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7020
gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 7080
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 7140
ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7200
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7260
gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 7320
tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 7380
agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 7440
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7500
cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7560
ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7620
ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 7680
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 7740
ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 7800
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 7860
gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7920
atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7980
ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8040
gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8100
tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8160
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8220
taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 8280
gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 8340
gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 8400
ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 8460
aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8520
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8580
cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 8640
actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 8700
caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 8760
gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 8820
ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 8880
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8940
tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9000
gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9060
cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 9120
ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 9180
gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 9240
aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 9300
catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 9360
taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt 9420
aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 9480
taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 9540
actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 9600
cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 9660
aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 9720
ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 9780
ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 9840
gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9900
gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9960
agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact 10020
ata 10023
<210> 6
<211> 10338
<212> DNA
<213> PVX/KSK*2(PVX/KSK*2)
<400> 6
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatacgcgta tattctgccc aaattcgcga 5700
ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg gtcgtgggtt 5760
cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg gtccagtgct 5820
tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc gcccgtctag 5880
ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc cccacggtgg 5940
gcgcccgggc atcaccatca ccatcactac ggccggcgca tgaagggcgc gccaatagga 6000
tccataaccg gtgcccgtct agctcagttg gtagagcgca aggctcttaa ccttgtggtc 6060
gtgggttcga gccccacggt gggcgaagct tgacgcgacc gaaatggtga aggacgggtc 6120
cagtgcttcg gcactgttga gtagagtgtg agctccgtaa ctggtcgcgt cgcatgcgcc 6180
cgtctagctc agttggtaga gcgcaaggct cttaaccttg tggtcgtggg ttcgagcccc 6240
acggtgggcg cccgggatac tgcagataat taattaacaa cctgggcctc atgtccggat 6300
gatatcgtcg accgccgatg aacggttaag tttccattga tactcgaaag atgtcagcac 6360
cagctagcac aacacagccc atagggtcaa ctacctcaac taccacaaaa actgcaggcg 6420
caactcctgc cacagcttca ggcctgttca ccatcccgga tggggatttc tttagtacag 6480
cccgtgccat agtagccagc aatgctgtcg caacaaatga ggacctcagc aagattgagg 6540
ctatttggaa ggacatgaag gtgcccacag acactatggc acaggctgct tgggacttag 6600
tcagacactg tgctgatgta ggatcatccg ctcaaacaga aatgatagat acaggtccct 6660
attccaacgg catcagcaga gctagactgg cagcagcaat taaagaggtg tgcacactta 6720
ggcaattttg catgaagtat gctccagtgg tatggaactg gatgttaact aacaacagtc 6780
cacctgctaa ctggcaagca caaggtttca agcctgagca caaattcgct gcattcgact 6840
tcttcaatgg agtcaccaac ccagctgcca tcatgcccaa agaggggctc atccggccac 6900
cgtctgaagc tgaaatgaat gctgcccaaa ctgctgcctt tgtgaagatt acaaaggcca 6960
gggcacaatc caacgacttt gccagcctag atgcagctgt cactcgaggt cgtatcactg 7020
gaacaacaac cgctgaggct gttgtcactc taccaccacc ataactacgt ctacataacc 7080
gacgcctacc ccagtttcat agtattttct ggtttgattg tatgaataat ataaataaaa 7140
aaaaaaaaaa aaaaaaaaaa ctagtggtac cgagctcggt accccatgca agcttttgtt 7200
ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7260
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7320
cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7380
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7440
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7500
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7560
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7620
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7680
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7740
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7800
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7860
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7920
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7980
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8040
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 8100
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8160
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8220
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8280
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8340
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8400
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8460
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8520
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8580
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8640
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8700
acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8760
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8820
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8880
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8940
ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 9000
gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 9060
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 9120
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 9180
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9240
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9300
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9360
ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 9420
cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 9480
acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 9540
atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 9600
ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 9660
taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt 9720
gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 9780
cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 9840
ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 9900
ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 9960
gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 10020
aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 10080
gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 10140
gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10200
ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10260
aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 10320
gtaatacgac tcactata 10338
<210> 7
<211> 10654
<212> DNA
<213> PVX/KSK*3(PVX/KSK*3)
<400> 7
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatacgcgta tattctgccc aaattcgcga 5700
ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg gtcgtgggtt 5760
cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg gtccagtgct 5820
tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc gcccgtctag 5880
ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc cccacggtgg 5940
gcgcccgggc atcaccatca ccatcactac ggccggcgca tgaagggcgc gccaatagga 6000
tccataaccg gtgcccgtct agctcagttg gtagagcgca aggctcttaa ccttgtggtc 6060
gtgggttcga gccccacggt gggcgaagct tgacgcgacc gaaatggtga aggacgggtc 6120
cagtgcttcg gcactgttga gtagagtgtg agctccgtaa ctggtcgcgt cgcatgcgcc 6180
cgtctagctc agttggtaga gcgcaaggct cttaaccttg tggtcgtggg ttcgagcccc 6240
acggtgggcg cccgggatac tgcagataat taattaacaa cctgggcctc atgtccggat 6300
gatatccgca tgaagggcgc gccaatagga tccataaccg gtgcccgtct agctcagttg 6360
gtagagcgca aggctcttaa ccttgtggtc gtgggttcga gccccacggt gggcgaagct 6420
tgacgcgacc gaaatggtga aggacgggtc cagtgcttcg gcactgttga gtagagtgtg 6480
agctccgtaa ctggtcgcgt cgcatgcgcc cgtctagctc agttggtaga gcgcaaggct 6540
cttaaccttg tggtcgtggg ttcgagcccc acggtgggcg cccgggatac tgcagataat 6600
taattaacaa ccgggcctca tggtcgaccg ccgatgaacg gttaagtttc cattgatact 6660
cgaaagatgt cagcaccagc tagcacaaca cagcccatag ggtcaactac ctcaactacc 6720
acaaaaactg caggcgcaac tcctgccaca gcttcaggcc tgttcaccat cccggatggg 6780
gatttcttta gtacagcccg tgccatagta gccagcaatg ctgtcgcaac aaatgaggac 6840
ctcagcaaga ttgaggctat ttggaaggac atgaaggtgc ccacagacac tatggcacag 6900
gctgcttggg acttagtcag acactgtgct gatgtaggat catccgctca aacagaaatg 6960
atagatacag gtccctattc caacggcatc agcagagcta gactggcagc agcaattaaa 7020
gaggtgtgca cacttaggca attttgcatg aagtatgctc cagtggtatg gaactggatg 7080
ttaactaaca acagtccacc tgctaactgg caagcacaag gtttcaagcc tgagcacaaa 7140
ttcgctgcat tcgacttctt caatggagtc accaacccag ctgccatcat gcccaaagag 7200
gggctcatcc ggccaccgtc tgaagctgaa atgaatgctg cccaaactgc tgcctttgtg 7260
aagattacaa aggccagggc acaatccaac gactttgcca gcctagatgc agctgtcact 7320
cgaggtcgta tcactggaac aacaaccgct gaggctgttg tcactctacc accaccataa 7380
ctacgtctac ataaccgacg cctaccccag tttcatagta ttttctggtt tgattgtatg 7440
aataatataa ataaaaaaaa aaaaaaaaaa aaaaaactag tggtaccgag ctcggtaccc 7500
catgcaagct tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca 7560
tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 7620
agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 7680
cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7740
caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7800
tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7860
cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7920
aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7980
gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 8040
agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 8100
cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 8160
cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 8220
ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 8280
gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 8340
tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 8400
acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 8460
tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 8520
attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 8580
gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 8640
ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 8700
taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8760
ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8820
ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8880
gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8940
ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 9000
gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 9060
tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 9120
atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 9180
gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 9240
tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 9300
atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 9360
agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 9420
ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 9480
tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 9540
aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 9600
tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 9660
aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 9720
accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 9780
gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 9840
gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 9900
ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 9960
catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg 10020
taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 10080
accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 10140
tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 10200
aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 10260
gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 10320
ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 10380
gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 10440
ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact 10500
gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 10560
gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 10620
cgacggccag tgaattgtaa tacgactcac tata 10654
<210> 8
<211> 10970
<212> DNA
<213> PVX/KSK*4(PVX/KSK*4)
<400> 8
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatacgcgta tattctgccc aaattcgcga 5700
ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg gtcgtgggtt 5760
cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg gtccagtgct 5820
tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc gcccgtctag 5880
ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc cccacggtgg 5940
gcgcccgggc atcaccatca ccatcactac ggccggcgca tgaagggcgc gccaatagga 6000
tccataaccg gtgcccgtct agctcagttg gtagagcgca aggctcttaa ccttgtggtc 6060
gtgggttcga gccccacggt gggcgaagct tgacgcgacc gaaatggtga aggacgggtc 6120
cagtgcttcg gcactgttga gtagagtgtg agctccgtaa ctggtcgcgt cgcatgcgcc 6180
cgtctagctc agttggtaga gcgcaaggct cttaaccttg tggtcgtggg ttcgagcccc 6240
acggtgggcg cccgggatac tgcagataat taattaacaa cctgggcctc atgtccggac 6300
gcatgaaggg cgcgccaata ggatccataa ccggtgcccg tctagctcag ttggtagagc 6360
gcaaggctct taaccttgtg gtcgtgggtt cgagccccac ggtgggcgaa gcttgacgcg 6420
accgaaatgg tgaaggacgg gtccagtgct tcggcactgt tgagtagagt gtgagctccg 6480
taactggtcg cgtcgcatgc gcccgtctag ctcagttggt agagcgcaag gctcttaacc 6540
ttgtggtcgt gggttcgagc cccacggtgg gcgcccggga tactgcagat aattaattaa 6600
caacctgggc ctcatggata tccgcatgaa gggcgcgcca ataggatcca taaccggtgc 6660
ccgtctagct cagttggtag agcgcaaggc tcttaacctt gtggtcgtgg gttcgagccc 6720
cacggtgggc gaagcttgac gcgaccgaaa tggtgaagga cgggtccagt gcttcggcac 6780
tgttgagtag agtgtgagct ccgtaactgg tcgcgtcgca tgcgcccgtc tagctcagtt 6840
ggtagagcgc aaggctctta accttgtggt cgtgggttcg agccccacgg tgggcgcccg 6900
ggatactgca gataattaat taacaaccgg gcctcatggt cgaccgccga tgaacggtta 6960
agtttccatt gatactcgaa agatgtcagc accagctagc acaacacagc ccatagggtc 7020
aactacctca actaccacaa aaactgcagg cgcaactcct gccacagctt caggcctgtt 7080
caccatcccg gatggggatt tctttagtac agcccgtgcc atagtagcca gcaatgctgt 7140
cgcaacaaat gaggacctca gcaagattga ggctatttgg aaggacatga aggtgcccac 7200
agacactatg gcacaggctg cttgggactt agtcagacac tgtgctgatg taggatcatc 7260
cgctcaaaca gaaatgatag atacaggtcc ctattccaac ggcatcagca gagctagact 7320
ggcagcagca attaaagagg tgtgcacact taggcaattt tgcatgaagt atgctccagt 7380
ggtatggaac tggatgttaa ctaacaacag tccacctgct aactggcaag cacaaggttt 7440
caagcctgag cacaaattcg ctgcattcga cttcttcaat ggagtcacca acccagctgc 7500
catcatgccc aaagaggggc tcatccggcc accgtctgaa gctgaaatga atgctgccca 7560
aactgctgcc tttgtgaaga ttacaaaggc cagggcacaa tccaacgact ttgccagcct 7620
agatgcagct gtcactcgag gtcgtatcac tggaacaaca accgctgagg ctgttgtcac 7680
tctaccacca ccataactac gtctacataa ccgacgccta ccccagtttc atagtatttt 7740
ctggtttgat tgtatgaata atataaataa aaaaaaaaaa aaaaaaaaaa aactagtggt 7800
accgagctcg gtaccccatg caagcttttg ttccctttag tgagggttaa ttccgagctt 7860
ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7920
caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7980
cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 8040
gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 8100
ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 8160
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 8220
agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 8280
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 8340
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 8400
tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8460
gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8520
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8580
tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8640
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8700
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8760
aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8820
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8880
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8940
attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 9000
ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 9060
tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 9120
aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 9180
acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 9240
aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 9300
agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 9360
ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 9420
agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 9480
tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 9540
tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9600
attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9660
taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9720
aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9780
caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9840
gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9900
cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9960
tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 10020
acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 10080
gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 10140
cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 10200
cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 10260
tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 10320
ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 10380
atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 10440
tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 10500
gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 10560
ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 10620
aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 10680
gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 10740
gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg ccattcgcca 10800
ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 10860
ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 10920
tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata 10970
<210> 9
<211> 11287
<212> DNA
<213> PVX/KSK*5(PVX/KSK*5)
<400> 9
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatcgcatga agggcgcgcc aataggatcc 5700
ataaccggtg cccgtctagc tcagttggta gagcgcaagg ctcttaacct tgtggtcgtg 5760
ggttcgagcc ccacggtggg cgaagcttga cgcgaccgaa atggtgaagg acgggtccag 5820
tgcttcggca ctgttgagta gagtgtgagc tccgtaactg gtcgcgtcgc atgcgcccgt 5880
ctagctcagt tggtagagcg caaggctctt aaccttgtgg tcgtgggttc gagccccacg 5940
gtgggcgccc gggatactgc agataattaa ttaacaacct gggcctcatg acgcgtatat 6000
tctgcccaaa ttcgcgaccg gtgcccgtct agctcagttg gtagagcgca aggctcttaa 6060
ccttgtggtc gtgggttcga gccccacggt gggcgaagct tgacgcgacc gaaatggtga 6120
aggacgggtc cagtgcttcg gcactgttga gtagagtgtg agctccgtaa ctggtcgcgt 6180
cgcatgcgcc cgtctagctc agttggtaga gcgcaaggct cttaaccttg tggtcgtggg 6240
ttcgagcccc acggtgggcg cccgggcatc accatcacca tcactacggc cggcgcatga 6300
agggcgcgcc aataggatcc ataaccggtg cccgtctagc tcagttggta gagcgcaagg 6360
ctcttaacct tgtggtcgtg ggttcgagcc ccacggtggg cgaagcttga cgcgaccgaa 6420
atggtgaagg acgggtccag tgcttcggca ctgttgagta gagtgtgagc tccgtaactg 6480
gtcgcgtcgc atgcgcccgt ctagctcagt tggtagagcg caaggctctt aaccttgtgg 6540
tcgtgggttc gagccccacg gtgggcgccc gggatactgc agataattaa ttaacaacct 6600
gggcctcatg tccggacgca tgaagggcgc gccaatagga tccataaccg gtgcccgtct 6660
agctcagttg gtagagcgca aggctcttaa ccttgtggtc gtgggttcga gccccacggt 6720
gggcgaagct tgacgcgacc gaaatggtga aggacgggtc cagtgcttcg gcactgttga 6780
gtagagtgtg agctccgtaa ctggtcgcgt cgcatgcgcc cgtctagctc agttggtaga 6840
gcgcaaggct cttaaccttg tggtcgtggg ttcgagcccc acggtgggcg cccgggatac 6900
tgcagataat taattaacaa cctgggcctc atggatatcc gcatgaaggg cgcgccaata 6960
ggatccataa ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg 7020
gtcgtgggtt cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg 7080
gtccagtgct tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc 7140
gcccgtctag ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc 7200
cccacggtgg gcgcccggga tactgcagat aattaattaa caaccgggcc tcatggtcga 7260
ccgccgatga acggttaagt ttccattgat actcgaaaga tgtcagcacc agctagcaca 7320
acacagccca tagggtcaac tacctcaact accacaaaaa ctgcaggcgc aactcctgcc 7380
acagcttcag gcctgttcac catcccggat ggggatttct ttagtacagc ccgtgccata 7440
gtagccagca atgctgtcgc aacaaatgag gacctcagca agattgaggc tatttggaag 7500
gacatgaagg tgcccacaga cactatggca caggctgctt gggacttagt cagacactgt 7560
gctgatgtag gatcatccgc tcaaacagaa atgatagata caggtcccta ttccaacggc 7620
atcagcagag ctagactggc agcagcaatt aaagaggtgt gcacacttag gcaattttgc 7680
atgaagtatg ctccagtggt atggaactgg atgttaacta acaacagtcc acctgctaac 7740
tggcaagcac aaggtttcaa gcctgagcac aaattcgctg cattcgactt cttcaatgga 7800
gtcaccaacc cagctgccat catgcccaaa gaggggctca tccggccacc gtctgaagct 7860
gaaatgaatg ctgcccaaac tgctgccttt gtgaagatta caaaggccag ggcacaatcc 7920
aacgactttg ccagcctaga tgcagctgtc actcgaggtc gtatcactgg aacaacaacc 7980
gctgaggctg ttgtcactct accaccacca taactacgtc tacataaccg acgcctaccc 8040
cagtttcata gtattttctg gtttgattgt atgaataata taaataaaaa aaaaaaaaaa 8100
aaaaaaaaac tagtggtacc gagctcggta ccccatgcaa gcttttgttc cctttagtga 8160
gggttaattc cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 8220
ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 8280
taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 8340
aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 8400
attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8460
cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8520
gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8580
ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8640
agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8700
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8760
ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8820
gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8880
ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8940
gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 9000
aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 9060
aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 9120
ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 9180
gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 9240
gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 9300
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 9360
ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9420
ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9480
atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9540
ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9600
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9660
attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9720
tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9780
ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9840
gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9900
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9960
gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 10020
aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 10080
taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 10140
tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 10200
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 10260
atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 10320
tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 10380
aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 10440
ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 10500
agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 10560
gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 10620
tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg 10680
cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 10740
cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 10800
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 10860
atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 10920
cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 10980
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 11040
tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 11100
cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 11160
cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 11220
cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact 11280
cactata 11287
<210> 10
<211> 10529
<212> DNA
<213> PVX/AtFT-KSK(PVX/AtFT-KSK)
<400> 10
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatatgtcta taaatataag agaccctctt 5700
atagtaagca gagttgttgg agacgttctt gatccgttta atagatcaat cactctaaag 5760
gttacttatg gccaaagaga ggtgactaat ggcttggatc taaggccttc tcaggttcaa 5820
aacaagccaa gagttgagat tggtggagaa gacctcagga acttctatac tttggttatg 5880
gtggatccag atgttccaag tcctagcaac cctcacctcc gagaatatct ccattggttg 5940
gtgactgata tccctgctac aactggaaca acctttggca atgagattgt gtgttacgaa 6000
aatccaagtc ccactgcagg aattcatcgt gtcgtgttta tattgtttcg acagcttggc 6060
aggcaaacag tgtatgcacc agggtggcgc cagaacttca acactcgcga gtttgctgag 6120
atctacaatc tcggccttcc cgtggccgca gttttctaca attgtcagag ggagagtggc 6180
tgcggaggaa gaagacttta gcggccgata ttctgcccaa attcgcgacc ggtgcccgtc 6240
tagctcagtt ggtagagcgc aaggctctta accttgtggt cgtgggttcg agccccacgg 6300
tgggcgaagc ttgacgcgac cgaaatggtg aaggacgggt ccagtgcttc ggcactgttg 6360
agtagagtgt gagctccgta actggtcgcg tcgcatgcgc ccgtctagct cagttggtag 6420
agcgcaaggc tcttaacctt gtggtcgtgg gttcgagccc cacggtgggc gcccgggcat 6480
caccatcacc atcactagtc gaccgccgat gaacggttaa gtttccattg atactcgaaa 6540
gatgtcagca ccagctagca caacacagcc catagggtca actacctcaa ctaccacaaa 6600
aactgcaggc gcaactcctg ccacagcttc aggcctgttc accatcccgg atggggattt 6660
ctttagtaca gcccgtgcca tagtagccag caatgctgtc gcaacaaatg aggacctcag 6720
caagattgag gctatttgga aggacatgaa ggtgcccaca gacactatgg cacaggctgc 6780
ttgggactta gtcagacact gtgctgatgt aggatcatcc gctcaaacag aaatgataga 6840
tacaggtccc tattccaacg gcatcagcag agctagactg gcagcagcaa ttaaagaggt 6900
gtgcacactt aggcaatttt gcatgaagta tgctccagtg gtatggaact ggatgttaac 6960
taacaacagt ccacctgcta actggcaagc acaaggtttc aagcctgagc acaaattcgc 7020
tgcattcgac ttcttcaatg gagtcaccaa cccagctgcc atcatgccca aagaggggct 7080
catccggcca ccgtctgaag ctgaaatgaa tgctgcccaa actgctgcct ttgtgaagat 7140
tacaaaggcc agggcacaat ccaacgactt tgccagccta gatgcagctg tcactcgagg 7200
tcgtatcact ggaacaacaa ccgctgaggc tgttgtcact ctaccaccac cataactacg 7260
tctacataac cgacgcctac cccagtttca tagtattttc tggtttgatt gtatgaataa 7320
tataaataaa aaaaaaaaaa aaaaaaaaaa actagtggta ccgagctcgg taccccatgc 7380
aagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct 7440
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7500
aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7560
actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7620
cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7680
gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7740
atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7800
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7860
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7920
ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7980
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8040
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8100
cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 8160
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 8220
aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 8280
atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8340
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8400
gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8460
gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8520
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8580
ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8640
tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8700
accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8760
atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8820
cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8880
tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8940
tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9000
gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9060
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9120
aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9180
gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9240
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9300
gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9360
tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9420
aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9480
catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9540
acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9600
tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9660
tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9720
tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9780
gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9840
gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaaac 9900
gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 9960
taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 10020
gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 10080
cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 10140
ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 10200
gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 10260
ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 10320
cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg 10380
gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 10440
gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 10500
gccagtgaat tgtaatacga ctcactata 10529
<210> 11
<211> 10529
<212> DNA
<213> PVX/mAtFT-KSK(PVX/mAtFT-KSK)
<400> 11
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gattagtcta taaatataag agaccctctt 5700
atagtaagca gagttgttgg agacgttctt gatccgttta atagatcaat cactctaaag 5760
gttacttatg gccaaagaga ggtgactaat ggcttggatc taaggccttc tcaggttcaa 5820
aacaagccaa gagttgagat tggtggagaa gacctcagga acttctatac tttggttatg 5880
gtggatccag atgttccaag tcctagcaac cctcacctcc gagaatatct ccattggttg 5940
gtgactgata tccctgctac aactggaaca acctttggca atgagattgt gtgttacgaa 6000
aatccaagtc ccactgcagg aattcatcgt gtcgtgttta tattgtttcg acagcttggc 6060
aggcaaacag tgtatgcacc agggtggcgc cagaacttca acactcgcga gtttgctgag 6120
atctacaatc tcggccttcc cgtggccgca gttttctaca attgtcagag ggagagtggc 6180
tgcggaggaa gaagacttta gcggccgata ttctgcccaa attcgcgacc ggtgcccgtc 6240
tagctcagtt ggtagagcgc aaggctctta accttgtggt cgtgggttcg agccccacgg 6300
tgggcgaagc ttgacgcgac cgaaatggtg aaggacgggt ccagtgcttc ggcactgttg 6360
agtagagtgt gagctccgta actggtcgcg tcgcatgcgc ccgtctagct cagttggtag 6420
agcgcaaggc tcttaacctt gtggtcgtgg gttcgagccc cacggtgggc gcccgggcat 6480
caccatcacc atcactagtc gaccgccgat gaacggttaa gtttccattg atactcgaaa 6540
gatgtcagca ccagctagca caacacagcc catagggtca actacctcaa ctaccacaaa 6600
aactgcaggc gcaactcctg ccacagcttc aggcctgttc accatcccgg atggggattt 6660
ctttagtaca gcccgtgcca tagtagccag caatgctgtc gcaacaaatg aggacctcag 6720
caagattgag gctatttgga aggacatgaa ggtgcccaca gacactatgg cacaggctgc 6780
ttgggactta gtcagacact gtgctgatgt aggatcatcc gctcaaacag aaatgataga 6840
tacaggtccc tattccaacg gcatcagcag agctagactg gcagcagcaa ttaaagaggt 6900
gtgcacactt aggcaatttt gcatgaagta tgctccagtg gtatggaact ggatgttaac 6960
taacaacagt ccacctgcta actggcaagc acaaggtttc aagcctgagc acaaattcgc 7020
tgcattcgac ttcttcaatg gagtcaccaa cccagctgcc atcatgccca aagaggggct 7080
catccggcca ccgtctgaag ctgaaatgaa tgctgcccaa actgctgcct ttgtgaagat 7140
tacaaaggcc agggcacaat ccaacgactt tgccagccta gatgcagctg tcactcgagg 7200
tcgtatcact ggaacaacaa ccgctgaggc tgttgtcact ctaccaccac cataactacg 7260
tctacataac cgacgcctac cccagtttca tagtattttc tggtttgatt gtatgaataa 7320
tataaataaa aaaaaaaaaa aaaaaaaaaa actagtggta ccgagctcgg taccccatgc 7380
aagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct 7440
gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7500
aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7560
actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7620
cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7680
gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7740
atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7800
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7860
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7920
ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7980
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8040
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8100
cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 8160
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 8220
aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 8280
atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8340
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8400
gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8460
gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8520
ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8580
ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8640
tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8700
accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8760
atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8820
cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8880
tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8940
tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9000
gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9060
agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9120
aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9180
gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9240
tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9300
gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9360
tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9420
aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9480
catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9540
acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9600
tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9660
tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9720
tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9780
gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9840
gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaaac 9900
gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 9960
taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 10020
gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 10080
cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 10140
ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 10200
gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 10260
ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 10320
cttaatgcgc cgctacaggg cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg 10380
gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 10440
gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 10500
gccagtgaat tgtaatacga ctcactata 10529
<210> 12
<211> 10541
<212> DNA
<213> PVX/AtTFL1-KSK(PVX/AtTFL1-KSK)
<400> 12
gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc 60
cgcttgaaaa agaaagttta acaaatggcc aaggtgcgcg aggtttacca atcttttaca 120
gactccacca caaaaactct catccaagat gaggcttata gaaacattcg ccccatcatg 180
gaaaaacaca aactagctaa cccttacgct caaacggttg aagcggctaa tgatctagag 240
gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag 300
accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca 360
tttatgtttc ttaaacccag aaagctaaac tacatgagaa gaaacccgcg gatcaaggac 420
attttccaaa atgttgccat tgaaccaaga gacgtagcca ggtaccccaa ggaaacaata 480
attgacaaac tcacagagat cacaacggaa acagcataca ttagtgacac tctgcacttc 540
ttggatccga gctacatagt ggagacattc caaaactgcc caaaattgca aacattgtat 600
gcgaccttag ttctccccgt tgaggcagcc tttaaaatgg aaagcactca cccgaacata 660
tacagcctca aatacttcgg agatggtttc cagtatatac caggcaacca tggtggcggg 720
gcataccatc atgaattcgc tcatctacaa tggctcaaag tgggaaagat caagtggagg 780
gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac 840
acagtgacag tacagttgca ggaatcgttc gcggcaaacc acttgtactg catcaggaga 900
ggagacttgc tcacaccgga ggtgcgcact ttcggccaac ctgacaggta cgtgattcca 960
ccacagatct tcctcccaaa agttcacaac tgcaagaagc cgattctcaa gaaaactatg 1020
atgcagctct tcttgtatgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc 1080
aaagtcagac aattaattaa atcatctgac ttggacaaat actctgctgt ggaactggtt 1140
tacttagtaa gctacatgga gttccttgcc gatttacaag ctaccacctg cttctcagac 1200
acactttctg gtggcttgct aacaaagacc cttgcaccgg tgagggcttg gatacaagag 1260
aaaaagatgc agctgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc 1320
cacccggtgg atttttcttt caaagtggaa acttgggact tcagattcca ccccttgcaa 1380
gcgtggaaag ccttccgacc aagggaagtg tcggatgtag aggaaatgga aagtttgttc 1440
tcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcggt aaacgcagag 1500
gaagatttag ctgcaatcag gaaaacgccc gagatggatg tcggtcaaga agttaaagag 1560
cctgcaggag acagaaatca atactcaaac cctgcagaaa ctttcctcaa caagctccac 1620
aggaaacaca gtagggaggt gaaacaccag gccgcaaaga aagctaaacg cctagctgaa 1680
atccaggagt caatgagagc tgaaggtgat gccgaaccaa atgaaataag cgggacgatg 1740
ggggcaatac ccagcaacgc cgaacttcct ggcacgaatg atgccagaca agaactcaca 1800
ctcccaacca ctaaacctgt ccctgcaagg tgggaagatg cttcattcac agattctagt 1860
gtggaagagg agcaggttaa actccttgga aaagaaaccg ttgaaacagc gacgcaacaa 1920
gtcatcgaag gacttccttg gaaacactgg attcctcaat taaatgctgt tggattcaag 1980
gcgctggaaa ttcagaggga taggagtgga acaatgatca tgcccatcac agaaatggtc 2040
tccgggctgg aaaaagagga cttccctgaa ggaactccaa aagagttggc acgagaattg 2100
ttcgctatga acagaagccc tgccaccatc cctttggacc tgcttagagc cagagactac 2160
ggcagtgatg taaagaacaa gagaattggt gccatcacaa agacacaggc aacgagttgg 2220
ggcgaatact tgacaggaaa gatagaaagc ttaactgaga ggaaagttgc gacttgtgtc 2280
attcatggag ctggaggttc tggaaaaagt catgccatcc agaaggcatt gagagaaatt 2340
ggcaagggct cggacatcac tgtagtcctg ccgaccaatg aactgcggct agattggagt 2400
aagaaagtgc ctaacactga gccctatatg ttcaagacct ctgaaaaggc gttaattggg 2460
ggaacaggca gcatagtcat ctttgacgat tactcaaaac ttcctcccgg ttacatagaa 2520
gccttagtct gtttctactc taaaatcaag ctaatcattc taacaggaga tagcagacaa 2580
agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcaacagag 2640
tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagatctt 2700
gcgaacatgc ttggtgtcta cagtgagaga acgggagtca ccgaaatcag catgagcgcc 2760
gagttcttag aaggaatccc aactttggta ccctcggatg agaagagaaa gctgtacatg 2820
ggcaccggga ggaatgacac gttcacatac gctggatgcc aggggctaac taagccgaag 2880
gtacaaatag tgttggacca caacacccaa gtgtgtagcg cgaatgtgat gtacacggca 2940
ctttctagag ccaccgatag gattcacttc gtgaacacaa gtgcaaattc ctctgccttc 3000
tgggaaaagt tggacagcac cccttacctc aagactttcc tatcagtggt gagagaacaa 3060
gcactcaggg agtacgagcc ggcagaggca gagccaattc aagagcctga gccccagaca 3120
cacatgtgtg tcgagaatga ggagtccgtg ctagaagagt acaaagagga actcttggaa 3180
aagtttgaca gagagatcca ctctgaatcc catggtcatt caaactgtgt ccaaactgaa 3240
gacacaacca ttcagttgtt ttcgcatcaa caagcaaaag atgagaccct cctctgggcg 3300
actatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcctgagc 3360
aagaaggaca ttggggacgt tctgttttta aactaccaaa aagctatggg tttacccaaa 3420
gagcgtattc ctttttccca agaggtctgg gaagcttgtg cccacgaagt acaaagcaag 3480
tacctcagca agtcaaagtg caacttgatc aatgggactg tgagacagag cccagacttc 3540
gatgaaaata agattatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaaacta 3600
ggtctaccca agattaagcc aggtcaaacc atagcagcct tttaccagca gactgtgatg 3660
ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa 3720
gtcttcataa actgtgagac cacgccagat gacatgtctg catgggcctt gaacaactgg 3780
aatttcagca gacctagctt ggctaatgac tacacagctt tcgaccagtc tcaggatgga 3840
gccatgttgc aatttgaggt gctcaaagcc aaacaccact gcataccaga ggaaatcatt 3900
caggcataca tagatattaa gactaatgca cagattttcc taggcacgtt atcaattatg 3960
cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc 4020
catacaaagt ttgacatccc agccggaact gctcaagttt atgcaggaga cgactccgca 4080
ctggactgtg ttccagaagt gaagcatagt ttccacaggc ttgaggacaa attactccta 4140
aagtcaaagc ctgtaatcac gcagcaaaag aagggcagtt ggcctgagtt ttgtggttgg 4200
ctgatcacac caaaaggggt gatgaaagac ccaattaagc tccatgttag cttaaaattg 4260
gctgaagcta agggtgaact caagaaatgt caagattcct atgaaattga tctgagttat 4320
gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacac 4380
acactcactt gcagaacact aatcaagtca gggagaggca ctgtctcact ttcccgcctc 4440
agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc 4500
agtagtttga aaagtttagg ttattctagg acttccaaat ctttagattc aggacctttg 4560
gtagtacatg cagtagccgg agccggtaag tccacagccc taaggaagtt gatcctcaga 4620
cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga 4680
ggcatacaga agccaggacc tattcctgag ggcaacttcg caatcctcga tgagtatact 4740
ttggacaaca ccacaaggaa ctctaaccag gcactttttg ctgaccctta tcaggcaccg 4800
gagtttagcc tagagcccca cttctacttg gaaacatcat ttcgagttcc gaggaaagtg 4860
gcagatttga tagctggctg tggcttcgat ttcgagacca actcaccgga agaagggcac 4920
ttagagatca ctggcatatt caaagggccc ctactcggaa aggtgatagc cattgatgag 4980
gagtctgaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgacg 5040
ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag 5100
tccacagctt tctacaacgc tatcaccagg tcaaagggat tgacatatgt ccgcgcaggg 5160
ccataggctg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcatt 5220
tgctttagtt tcaattacct ttctgctttc tagaaatagc ttaccccacg tcggtgacaa 5280
cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa 5340
ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc 5400
cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac 5460
ttgtgcttgt ggtaacaatc atagcagtca ttagcacttc cttagtgagg actgaacctt 5520
gtgtcatcaa gattactggg gaatcaatca cagtgttggc ttgcaaacta gatgcagaaa 5580
ccataagggc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat 5640
actcgaaaga ggtcagcacc agctagcatc gatatggaga atatgggaac tagagtgata 5700
gagccattga taatggggag agtggtagga gatgttcttg atttcttcac tccaacaact 5760
aagatgaatg ttagttataa caagaagcaa gtctccaatg gccatgagct ctttccttct 5820
tctgtttcct ccaagcctag ggttgagatc catggtggtg atctcagatc cttcttcact 5880
ttggtgatga tagacccaga tgttccaggt cctagtgacc cctttctaaa agaacacctg 5940
cactggatcg ttacaaacat tcccggcaca acagatgcta cgtttggcaa agaggtggtg 6000
agctatgaat tgccaaggcc aagcataggg atacataggt ttgtgtttgt tctgttcagg 6060
cagaagcaaa gacgtgttat ctttcctaat atcccttcga gagatcactt caacactcgt 6120
aaatttgcgg tcgagtatga tcttggtctc cctgtcgcgg ccgtcttctt taacgcacaa 6180
agagaaaccg ctgcacgcaa acgctagacg cgtcggccga tattctgccc aaattcgcga 6240
ccggtgcccg tctagctcag ttggtagagc gcaaggctct taaccttgtg gtcgtgggtt 6300
cgagccccac ggtgggcgaa gcttgacgcg accgaaatgg tgaaggacgg gtccagtgct 6360
tcggcactgt tgagtagagt gtgagctccg taactggtcg cgtcgcatgc gcccgtctag 6420
ctcagttggt agagcgcaag gctcttaacc ttgtggtcgt gggttcgagc cccacggtgg 6480
gcgcccgggc atcaccatca ccatcactag tcgaccgccg atgaacggtt aagtttccat 6540
tgatactcga aagatgtcag caccagctag cacaacacag cccatagggt caactacctc 6600
aactaccaca aaaactgcag gcgcaactcc tgccacagct tcaggcctgt tcaccatccc 6660
ggatggggat ttctttagta cagcccgtgc catagtagcc agcaatgctg tcgcaacaaa 6720
tgaggacctc agcaagattg aggctatttg gaaggacatg aaggtgccca cagacactat 6780
ggcacaggct gcttgggact tagtcagaca ctgtgctgat gtaggatcat ccgctcaaac 6840
agaaatgata gatacaggtc cctattccaa cggcatcagc agagctagac tggcagcagc 6900
aattaaagag gtgtgcacac ttaggcaatt ttgcatgaag tatgctccag tggtatggaa 6960
ctggatgtta actaacaaca gtccacctgc taactggcaa gcacaaggtt tcaagcctga 7020
gcacaaattc gctgcattcg acttcttcaa tggagtcacc aacccagctg ccatcatgcc 7080
caaagagggg ctcatccggc caccgtctga agctgaaatg aatgctgccc aaactgctgc 7140
ctttgtgaag attacaaagg ccagggcaca atccaacgac tttgccagcc tagatgcagc 7200
tgtcactcga ggtcgtatca ctggaacaac aaccgctgag gctgttgtca ctctaccacc 7260
accataacta cgtctacata accgacgcct accccagttt catagtattt tctggtttga 7320
ttgtatgaat aatataaata aaaaaaaaaa aaaaaaaaaa aaactagtgg taccgagctc 7380
ggtaccccat gcaagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc 7440
atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7500
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7560
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7620
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7680
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7740
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7800
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7860
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7920
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7980
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8040
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8100
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8160
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8220
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8280
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8340
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8400
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8460
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8520
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8580
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8640
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8700
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8760
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8820
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8880
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8940
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9000
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9060
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9120
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9180
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9240
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9300
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9360
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9420
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9480
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9540
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9600
ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9660
tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9720
ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9780
gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9840
agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9900
gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 9960
ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 10020
atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 10080
aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 10140
taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 10200
ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 10260
gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 10320
acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc attcaggctg 10380
cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 10440
gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 10500
tgtaaaacga cggccagtga attgtaatac gactcactat a 10541

Claims (1)

  1. The application of DFHBI as a plant RNA fluorescent dye for realizing non-specific binding with Spinach-free green plant RNA is PVX/KK, PVX/KSK 1, PVX/KSK 2, PVX/KSK 3, PVX/KSK 4 and PVX/KSK 5 vectors, wherein K represents lysine transport RNA, S represents Spinach RNA, 1, 2, 3, 4, 5 represents that 1 to 5 KSKs are connected into the PVX vectors in series, the nucleotide sequence of the PVX/KSK 1 is shown as SEQ ID No. 5, the nucleotide sequence of the PVX/KSK 2 is shown as SEQ ID No. 6, the nucleotide sequence of the PVX/KSK 3 is shown as SEQ ID No. 7, the nucleotide sequence of the PVX/KSK 4 is shown as SEQ ID No. 8, and the nucleotide sequence of the PVX/KSK 5 is shown as SEQ ID No. 5As shown in SEQ ID No. 9, the DFHBI molecular formula is as follows: c 12 H 10 F 2 N 2 O 2 The molecular weight is: 252.22, the structural formula is shown below:
    Figure DEST_PATH_IMAGE001
    。
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102405212A (en) * 2009-02-18 2012-04-04 康奈尔大学 Coupled recognition/detection system for in vivo and in vitro use
WO2013016694A2 (en) * 2011-07-27 2013-01-31 Cornell University Methods for rna detection and quantification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102405212A (en) * 2009-02-18 2012-04-04 康奈尔大学 Coupled recognition/detection system for in vivo and in vitro use
WO2013016694A2 (en) * 2011-07-27 2013-01-31 Cornell University Methods for rna detection and quantification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Characterization of the Photophysical Behavior of DFHBI Derivatives: Fluorogenic Molecules that Illuminate the Spinach RNA Aptamer;Kalyan Santra等;《J. Phys. Chem. B》;20190226;第123卷;2536-2545 *
Fluorescent Monitoring of RNA Assembly and Processing Using the Split-Spinach Aptamer;Tucker A. Rogers等;《ACS Synth. Biol.》;20140429;第4卷;162-166 *
RNA Mimics of Green Fluorescent Protein;Jeremy S. Paige等;《SCIENCE》;20110729;第333卷;642-646 *

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