CN112205393A - Method for manufacturing large kelp adult specimen - Google Patents
Method for manufacturing large kelp adult specimen Download PDFInfo
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- CN112205393A CN112205393A CN202011107118.6A CN202011107118A CN112205393A CN 112205393 A CN112205393 A CN 112205393A CN 202011107118 A CN202011107118 A CN 202011107118A CN 112205393 A CN112205393 A CN 112205393A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The invention provides a method for preparing a large kelp adult specimen, which comprises the steps of cleaning the surface of kelp, sucking the water on the surface of the kelp, and drying the kelp by placing the kelp on a smooth and clean plate surface; after drying, cleaning the surface of the kelp by using sterilized seawater; then drying and then making a specimen; coating transparent vaseline on the inner side of a mould by using a polyvinyl chloride transparent plate, then uniformly mixing mixed glue solution prepared from epoxy resin and polyetheramine D-230, defoaming at the temperature of not more than 40 ℃, pouring the defoamed mixed glue solution into the mould, placing the treated kelp into the prepared mould after the depth of the mixed glue solution reaches 1-1.5cm, enabling the kelp to sink into the mixed glue solution, standing for 7-12h, covering the defoamed mixed glue solution on the kelp, and standing for 24-48 h until the specimen is solidified; and after the specimen is solidified, polishing the kelp specimen, and then coating UV glue to perform irradiation and solidification of a UV lamp to complete preparation of the specimen.
Description
Technical Field
The invention belongs to the technical field of preparation of large seaweed specimens, and particularly relates to a method for preparing large seaweed adult specimens.
Technical Field
Kelp (Laminaria japonica), also known as Dacron, kelp and Jiangchang cabbage, belongs to the phylum Phaeophyta, class Brownia, order Laminariales, family Laminariaceae, genus Laminaria, and is a perennial large brown algae. The kelp belongs to algae in the subarctic zone, is a special local species of the North Pacific ocean, and also has some kelp distribution in the Atlantic ocean. The kelp is rich in nutrition, contains rich laminarin, algin, fucoidin, brown algae starch and the like, has low calorie, moderate protein and rich mineral content, and is an ideal natural marine food. The kelp cultivation is carried out in China from the 50 th century, the seedling production is deeply explored, the artificial breeding and breeding technology of the kelp reaches the international leading level, and the kelp cultivation becomes an important economic algae species in China. In order to meet the industrial requirements, scientific researchers and farmers also breed a plurality of kelp strains with excellent properties, such as high-tolerance strains suitable for cultivation, more delicious food strains and the like. With the generation breeding and cultivation of the kelp, part of kelp strains die gradually, and although the die strains can not meet the requirements of the cultivation industry, the varieties of the kelp at different periods are indispensable to the ecological evolution of the kelp, so that the kelp is preserved in a specimen mode, and the kelp breeding method has important significance for the kelp. The kelp specimen at the present stage is mostly dry-pressed, the dry specimen prolongs the storage time of the specimen, and the specimen is subjected to plastic package in the last step, but the dried specimen has size limitation due to the size limitation of a plastic packaging machine, the size of the formed kelp specimen can reach 3-4m, the kelp specimen can be dried but can not be subjected to plastic packaging, and the plastic packaging technology of the large-size algae body is not broken at present.
At present, the size of the seaweed epoxy resin specimen is small, most of the seaweed epoxy resin specimens are manufactured by reusable silica gel molds, but the size of the silica gel molds in the market is small (the longest diameter is less than 0.5m), the silica gel molds are fixed and cannot be adjusted along with the change of algae, and the silica gel molds can only be purchased in large batch to meet the manufacture of the seaweed specimens with different specifications, and the manufacture of large seaweed adult specimens cannot be met due to the small size, the market can customize the silica gel molds, if the large silica gel molds are customized, the mold opening cost of the silica gel molds is five thousand yuan, and when the customized silica gel molds are used for manufacturing other specimens, the size cannot be adjusted again, and the whole large seaweed specimen manufacturing has high value, but the needed number is small, so the cost performance of the customized seaweed large silica gel mold is not high.
At present, in the process of manufacturing an algae epoxy resin specimen, an algae body is firstly dried, and a drying agent is mostly adopted for drying, because the drying agent can generate certain supporting and pressing effects on the algae body, the dried algae body is stretched, but in the drying process of the drying agent, the kelp is decolored due to the effect of the drying agent and the thinner folded edge of the kelp. Therefore, the kelp cannot be dried by a drying agent, and the kelp can be directly dried by natural air to cause more folds of the kelp body.
Disclosure of Invention
The invention provides a method for manufacturing a large kelp adult algae specimen, the method can be used for manufacturing the large kelp adult algae specimen (1 m), the manufacturing process cannot damage algae pigment, and the prepared specimen finished product is beautiful, transparent, solid, easy to store and easy to transport.
The invention provides a method for manufacturing a large kelp adult specimen, which comprises the following steps.
1) Pretreatment of kelp samples:
cleaning the surface of the kelp, sucking water on the surface of the kelp, and drying the kelp on a smooth and clean board surface; after drying, cleaning the surface of the kelp by using sterilized seawater; then drying and then making a specimen;
the drying is preferably carried out by placing the kelp in a closed space and drying the kelp by using a dehumidifier;
2) manufacturing a mould:
a mold for preparing a specimen using a transparent polyvinyl chloride sheet,
the length of the die is a + (20-50) cm; the width is b + (20-50) cm; wherein a is the length of the kelp and b is the width of the kelp;
3) glue injection
Smearing transparent vaseline on the inner side of a mold, then uniformly mixing a mixed glue solution prepared from epoxy resin and polyetheramine D-230, defoaming at the temperature of not more than 40 ℃, pouring the defoamed mixed glue solution into the mold, placing the treated kelp in the prepared mold after the depth of the mixed glue solution reaches 1-1.5cm, enabling the kelp to sink into the mixed glue solution, placing for 7-12h, covering the defoamed mixed glue solution on the kelp, and standing for 24-48 h until a specimen is solidified;
wherein the mass ratio of the epoxy resin to the polyether amine D-230 is (2-4): 1;
4) stripping and polishing
And after the specimen is solidified, polishing the kelp specimen, and then coating UV glue to perform irradiation and solidification of a UV lamp to complete preparation of the specimen.
The method of the invention has the following advantages:
(1) the obtained specimen can better preserve the original form of algae, the step of drying the specimen to absorb water and press the specimen to a plane does not exist, the algae processing time is short, the color of the algae is smaller than the initial difference, the preparation period of the dried specimen is longer, the contact time of the algae and oxygen in the air is longer, the algae is oxidized, and the color is larger than the initial difference.
(2) The mould used in the method adopts the polyvinyl chloride transparent plate with relatively low price, the mould is manufactured according to different sizes of the algae, so that the specimen has more aesthetic, and the kelp adult with large size (>1m) can be manufactured by a splicing method. Compared with a customized silica gel mold, the cost performance is higher.
(3) The method of the invention effectively solves the problem of atomization formed on the surface of the algae body in the prior art for preparing the kelp specimen.
(4) The method comprises the steps of placing the kelp specimen in a closed room, drying the kelp specimen by using a dehumidifier, forming fewer unnatural wrinkles on the kelp than the kelp specimen dried by using natural wind, and simultaneously, recovering small wrinkles on the surface of the kelp specimen by using water for a short time after the drying is finished, wherein the recovery on the surface of the kelp specimen is finished in a short time so as to prevent the interior of the kelp specimen from absorbing water. Thereby better displaying the shape of the kelp.
Drawings
FIG. 1: a first schematic view of a polyvinyl chloride mold;
FIG. 2: a second schematic diagram of a polyvinyl chloride mold;
FIG. 3: a third schematic diagram of a polyvinyl chloride mold;
FIG. 4: photograph of kelp specimen 1 (specimen length 1 m);
FIG. 5: photograph of Laminaria japonica specimen 2 (specimen length: 3 m).
Detailed Description
In the research of the applicant, the mannitol overflows from the surface of a kelp adult when a kelp specimen is prepared, the mannitol is in a sticky liquid state when the kelp is fresh, a white atomized surface is formed on the surface of the kelp when the kelp specimen is embedded by epoxy resin, the observation of the kelp body is not facilitated, and the mannitol is changed into white powder after the kelp specimen is dehydrated, is adhered to the surface of the kelp specimen and is not dissolved in the epoxy resin, and the attractiveness of the specimen is also influenced.
In order to solve the problem, the applicant cleans the surface of the kelp by using sterilized seawater after drying; then drying and then making a specimen; thereby effectively solving the problems.
Meanwhile, the polyvinyl chloride mould is adopted, so that the problems of high counterfeiting and inconvenience in use of the existing silica gel mould are effectively solved.
The present invention will be described in detail below with reference to examples and the accompanying drawings.
Example 1
(1) Sample treatment of kelp
The kelp is cleaned by filtering seawater to remove dirt on the surface and mannitol, then the water on the surface of the kelp is sucked to be dry by using clean gauze, the kelp is placed on a smooth and clean board surface, the board surface is placed in a closed chamber and is dried by using a dehumidifier, the mannitol in the algae is further released in the drying process, a layer of white powder is formed on the surface of the kelp, after the kelp is air-dried, the algae is cleaned by using sterilized seawater, meanwhile, under the action of water, small folds on the surface of the algae are recovered, and the recovery of the surface of the kelp is completed in a short time, so that the water in the algae is prevented from being absorbed. And drying for 4 hours after recovery to prepare a specimen.
(2) Manufacture of moulds
The length of the die is a + (20-50) cm; the width is b + (20-50) cm; wherein a is the length of the kelp and b is the width of the kelp;
measuring the length and width of the kelp to be 100cm and 10cm respectively, and cutting the transparent polyvinyl chloride plate according to the measurement result, wherein the length and width of the transparent polyvinyl chloride plate are 140cm and 40 cm. Polyvinyl chloride was cut into 8cm from point A, B, C, D shown in fig. 1, wherein point A, B, C, D was 8cm from the long side, the transparent plate was folded up at four sides, and the shadow was overlapped and fixed by hot melt adhesive (fig. 2).
(3) Glue injection
A small amount of transparent vaseline is stuck by gauze and smeared on the inner side of the mould, so that demoulding is facilitated. Mixing epoxy resin and polyether amine D-230 in a weight ratio of 3: 1, defoaming at the temperature of not more than 40 ℃, pouring the defoamed mixed glue solution into a mold, placing the treated sample in the prepared mold after the depth of the mixed glue solution reaches 1-1.5cm, slightly pressing the sample with a glass rod to avoid retaining bubbles, placing for 7-12h, pouring the defoamed mixed glue solution glue-covered algae body into a film, and standing for 24-48 h until the sample is solidified. If the algae is large, pouring the glue solution for 2-3 times, wherein the interval time is 24h every time, so as to avoid forming lines because the glue is not solidified last time when pouring the glue again.
(4) Stripping and polishing
After the specimen is solidified, tearing the mold along the glue joint, tearing the mold from the specimen, polishing the obtained algae specimen, coating UV glue, and irradiating and curing the UV lamp to finish the preparation. The resulting product is shown in figure 4. The prepared specimen finished product is transparent in blank, and is integrally firm, white water mist is not formed on the surface of an alga body in the specimen, the alga body is not decolored, the color of the alga body is close to the color of a fresh alga body, the shape of the alga body is three-dimensional, the edge of the alga body is naturally bent, and the state of the alga body is close to the actual state of kelp in water. The specimen is placed for one month, the algae body is not changed, and the humidity and the temperature for storing the specimen have no influence on the specimen.
Example 2
(1) Sample treatment of kelp
The kelp is cleaned by filtering seawater to remove dirt on the surface and mannitol, then the water on the surface of the kelp is sucked to be dry by using clean gauze, the kelp is placed on a smooth and clean board surface, the board surface is placed in a closed chamber and is dried by using a dehumidifier, the mannitol in the kelp body is further released in the drying process, a layer of white powder is formed on the surface of the kelp, after the kelp is air-dried, the kelp body is cleaned by using sterilized seawater, meanwhile, small folds on the surface of the kelp body are recovered under the action of water, and the recovery of the surface of the kelp body is completed in a short time, so that the water in the kelp body is prevented from being absorbed. And drying for 4 hours after recovery to prepare a specimen.
(2) Manufacture of moulds
The length and the width of the kelp are measured to be 300cm and 35cm respectively, the polyvinyl chloride transparent plate is cut according to the measurement result, the length and the width of the required transparent plate are 350cm and 80cm, the factory transparent plate is 100cm multiplied by 240cm in maximum size, the width of the transparent plate can meet the requirement, but the length of the transparent plate needs to be spliced together by two plates, one plate is 240cm in length and the other plate is 90cm in length, as shown in figure 3, E, F is the splicing position of the plates, the back of the splicing position is attached by using transparent adhesive tapes, polyvinyl chloride is cut into 10cm from A, B, C, D, wherein the length of a A, B, C, D point is 10cm from the long side, the four sides of the transparent plate are folded and fixed by hot melt adhesives.
(3) Glue injection
A small amount of transparent vaseline is stuck by gauze and smeared on the inner side of the mould, so that demoulding is facilitated. Mixing epoxy resin and polyether amine D-230 in a weight ratio of 2.5: 1, defoaming at the temperature of not more than 40 ℃, pouring the defoamed mixed glue solution into a mold, placing the treated sample in the prepared mold after the depth of the mixed glue solution reaches 1-1.5cm, slightly pressing the sample with a glass rod to avoid retaining bubbles, placing for 7-12h, pouring the defoamed mixed glue solution glue-covered algae body into a film, and standing for 24-48 h until the sample is solidified. If the algae is large, pouring the glue solution for 2-3 times, wherein the interval time is 24h every time, so as to avoid forming lines because the glue is not solidified last time when pouring the glue again.
4. Stripping and polishing
After the specimen is solidified, tearing the mold along the glue joint, tearing the mold from the specimen, polishing the obtained algae specimen, coating UV glue, and irradiating and curing the UV lamp to finish the preparation. The final product is shown in fig. 5. The finished product of the specimen has large size, transparent blank, hard whole and no fracture deformation in the carrying process. White water mist is not formed on the surface of the algae in the specimen, the color is not decolored, the color is close to the color of a fresh algae, the shape of the algae is three-dimensional, the edge is naturally bent, and the state is close to the actual state of the kelp in water. The specimen is placed for one month, the algae body is not changed, and the humidity and the temperature for storing the specimen have no influence on the specimen.
Claims (4)
1. The method for manufacturing the large kelp adult specimen is characterized by comprising the following steps of:
1) pretreatment of kelp samples:
cleaning the surface of the kelp, sucking water on the surface of the kelp, and drying the kelp on a smooth and clean board surface; after drying, cleaning the surface of the kelp by using sterilized seawater; then drying and then making a specimen;
2) manufacturing a mould:
preparing a mold of a specimen using a transparent polyvinyl chloride plate;
3) glue injection
Smearing transparent vaseline on the inner side of a mold, then uniformly mixing a mixed glue solution prepared from epoxy resin and polyetheramine D-230, defoaming at the temperature of not more than 40 ℃, pouring the defoamed mixed glue solution into the mold, placing the treated kelp in the prepared mold after the depth of the mixed glue solution reaches 1-1.5cm, enabling the kelp to sink into the mixed glue solution, placing for 7-12h, covering the defoamed mixed glue solution on the kelp, and standing for 24-48 h until a specimen is solidified;
4) stripping and polishing
And after the specimen is solidified, polishing the kelp specimen, and then coating UV glue to perform irradiation and solidification of a UV lamp to complete preparation of the specimen.
2. The method according to claim 1, wherein the drying in 1) is carried out by placing the kelp in a closed space and drying the kelp with a dehumidifier.
3. The method of claim 1, wherein said mold of 2) has a length of a + (20-50) cm; the width is b + (20-50) cm; wherein a is the length of the kelp and b is the width of the kelp.
4. The method as claimed in claim 1, wherein the mass ratio of the epoxy resin to the polyetheramine D-230 in 3) is 2-4: 1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011107118.6A CN112205393B (en) | 2020-10-16 | 2020-10-16 | Method for manufacturing large kelp adult specimen |
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| CN202011107118.6A CN112205393B (en) | 2020-10-16 | 2020-10-16 | Method for manufacturing large kelp adult specimen |
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| CN112205393B CN112205393B (en) | 2022-03-15 |
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Citations (6)
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| CN110050785A (en) * | 2019-05-25 | 2019-07-26 | 宁波大学 | A kind of producing device and its application method of algae exsiccata |
| CN111328799A (en) * | 2020-04-27 | 2020-06-26 | 山东省海洋生物研究院 | Preparation method of mollusca algae specimen |
-
2020
- 2020-10-16 CN CN202011107118.6A patent/CN112205393B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06227903A (en) * | 1993-02-05 | 1994-08-16 | Kuraray Co Ltd | Specimen encapsulated with ionic compound composition |
| CN101548670A (en) * | 2009-04-21 | 2009-10-07 | 宁波大学 | Method for praparing varek specimen |
| CN102835390A (en) * | 2012-08-23 | 2012-12-26 | 黑龙江东方学院 | Preparation method of detachable plant three-dimensional specimen |
| CN108835106A (en) * | 2018-08-10 | 2018-11-20 | 山东省海洋生物研究院 | A kind of mold and its application method for Sargassum horneri seedling preparation of specimen |
| CN110050785A (en) * | 2019-05-25 | 2019-07-26 | 宁波大学 | A kind of producing device and its application method of algae exsiccata |
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Non-Patent Citations (1)
| Title |
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| 郭城孟等: "《自然野趣大观察 蕨类 超值版》", 31 July 2016, 福建科学技术出版社 * |
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