CN112204138A - Automatic dishwashing detergent composition - Google Patents
Automatic dishwashing detergent composition Download PDFInfo
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- CN112204138A CN112204138A CN201980035161.5A CN201980035161A CN112204138A CN 112204138 A CN112204138 A CN 112204138A CN 201980035161 A CN201980035161 A CN 201980035161A CN 112204138 A CN112204138 A CN 112204138A
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- 239000011133 lead Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- BQKYBHBRPYDELH-UHFFFAOYSA-N manganese;triazonane Chemical compound [Mn].C1CCCNNNCC1 BQKYBHBRPYDELH-UHFFFAOYSA-N 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 108010020132 microbial serine proteinases Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 125000002950 monocyclic group Chemical group 0.000 description 1
- JLCNIMCQBVMUIN-UHFFFAOYSA-N n-docosylprop-2-enamide Chemical compound CCCCCCCCCCCCCCCCCCCCCCNC(=O)C=C JLCNIMCQBVMUIN-UHFFFAOYSA-N 0.000 description 1
- XQPVIMDDIXCFFS-UHFFFAOYSA-N n-dodecylprop-2-enamide Chemical compound CCCCCCCCCCCCNC(=O)C=C XQPVIMDDIXCFFS-UHFFFAOYSA-N 0.000 description 1
- JKZQBSUPPNQHTK-UHFFFAOYSA-N n-ethyl-2-methylideneoctanamide Chemical compound CCCCCCC(=C)C(=O)NCC JKZQBSUPPNQHTK-UHFFFAOYSA-N 0.000 description 1
- SWPMNMYLORDLJE-UHFFFAOYSA-N n-ethylprop-2-enamide Chemical compound CCNC(=O)C=C SWPMNMYLORDLJE-UHFFFAOYSA-N 0.000 description 1
- YPHQUSNPXDGUHL-UHFFFAOYSA-N n-methylprop-2-enamide Chemical compound CNC(=O)C=C YPHQUSNPXDGUHL-UHFFFAOYSA-N 0.000 description 1
- CNWVYEGPPMQTKA-UHFFFAOYSA-N n-octadecylprop-2-enamide Chemical compound CCCCCCCCCCCCCCCCCCNC(=O)C=C CNWVYEGPPMQTKA-UHFFFAOYSA-N 0.000 description 1
- AWGZKFQMWZYCHF-UHFFFAOYSA-N n-octylprop-2-enamide Chemical compound CCCCCCCCNC(=O)C=C AWGZKFQMWZYCHF-UHFFFAOYSA-N 0.000 description 1
- XFHJDMUEHUHAJW-UHFFFAOYSA-N n-tert-butylprop-2-enamide Chemical compound CC(C)(C)NC(=O)C=C XFHJDMUEHUHAJW-UHFFFAOYSA-N 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000001451 organic peroxides Chemical class 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 150000002924 oxiranes Chemical group 0.000 description 1
- UFOIOXZLTXNHQH-UHFFFAOYSA-N oxolane-2,3,4,5-tetracarboxylic acid Chemical compound OC(=O)C1OC(C(O)=O)C(C(O)=O)C1C(O)=O UFOIOXZLTXNHQH-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- YWAKXRMUMFPDSH-UHFFFAOYSA-N pentene Chemical compound CCCC=C YWAKXRMUMFPDSH-UHFFFAOYSA-N 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 150000004965 peroxy acids Chemical class 0.000 description 1
- XCRBXWCUXJNEFX-UHFFFAOYSA-N peroxybenzoic acid Chemical class OOC(=O)C1=CC=CC=C1 XCRBXWCUXJNEFX-UHFFFAOYSA-N 0.000 description 1
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
- 125000005342 perphosphate group Chemical group 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- UIIIBRHUICCMAI-UHFFFAOYSA-N prop-2-ene-1-sulfonic acid Chemical compound OS(=O)(=O)CC=C UIIIBRHUICCMAI-UHFFFAOYSA-N 0.000 description 1
- 125000002572 propoxy group Chemical group [*]OC([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Chemical group CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000004180 red 2G Substances 0.000 description 1
- 239000013557 residual solvent Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
- 229910052911 sodium silicate Inorganic materials 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000000547 substituted alkyl group Chemical group 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005494 tarnishing Methods 0.000 description 1
- 108010075550 termamyl Proteins 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- YMBCJWGVCUEGHA-UHFFFAOYSA-M tetraethylammonium chloride Chemical compound [Cl-].CC[N+](CC)(CC)CC YMBCJWGVCUEGHA-UHFFFAOYSA-M 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- VWNRYDSLHLCGLG-NDNWHDOQSA-J tetrasodium;(2s)-2-[bis(carboxylatomethyl)amino]butanedioate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)C[C@@H](C([O-])=O)N(CC([O-])=O)CC([O-])=O VWNRYDSLHLCGLG-NDNWHDOQSA-J 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- LDHQCZJRKDOVOX-UHFFFAOYSA-N trans-crotonic acid Natural products CC=CC(O)=O LDHQCZJRKDOVOX-UHFFFAOYSA-N 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- 150000003624 transition metals Chemical group 0.000 description 1
- 229960002622 triacetin Drugs 0.000 description 1
- 150000003918 triazines Chemical class 0.000 description 1
- WEAPVABOECTMGR-UHFFFAOYSA-N triethyl 2-acetyloxypropane-1,2,3-tricarboxylate Chemical compound CCOC(=O)CC(C(=O)OCC)(OC(C)=O)CC(=O)OCC WEAPVABOECTMGR-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 125000004417 unsaturated alkyl group Chemical group 0.000 description 1
- 239000004474 valine Chemical group 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- NLVXSWCKKBEXTG-UHFFFAOYSA-N vinylsulfonic acid Chemical compound OS(=O)(=O)C=C NLVXSWCKKBEXTG-UHFFFAOYSA-N 0.000 description 1
- 239000004711 α-olefin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47L—DOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
- A47L15/00—Washing or rinsing machines for crockery or tableware
- A47L15/42—Details
- A47L15/44—Devices for adding cleaning agents; Devices for dispensing cleaning agents, rinsing aids or deodorants
- A47L15/4418—Devices for adding cleaning agents; Devices for dispensing cleaning agents, rinsing aids or deodorants in the form of liquids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/008—Polymeric surface-active agents
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/10—Carbonates ; Bicarbonates
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2075—Carboxylic acids-salts thereof
- C11D3/2086—Hydroxy carboxylic acids-salts thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin
- C11D3/225—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin etherified, e.g. CMC
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/33—Amino carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/39—Organic or inorganic per-compounds
- C11D3/3902—Organic or inorganic per-compounds combined with specific additives
- C11D3/3905—Bleach activators or bleach catalysts
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/39—Organic or inorganic per-compounds
- C11D3/3945—Organic per-compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/395—Bleaching agents
- C11D3/3951—Bleaching agents combined with specific additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
-
- C11D2111/14—
Abstract
The present invention provides an automatic dishwashing cleaning composition with a novel protease.
Description
Technical Field
The present invention relates to the field of detergents. In particular, the present invention relates to automatic dishwashing detergents comprising specific proteases. The compositions provide improved proteinaceous soil removal compared to compositions comprising conventional proteases.
Background
There is a continuing desire to improve the performance of automatic dishwashing compositions and their environmental characteristics.
Due to environmental problems, phosphates are gradually being replaced by biodegradable complexing agents. These complexing agents may have a strong binding capacity for metals and/or be used at high levels and may adversely affect the stability of the enzyme, in particular, the complexing agents may adversely affect the protease by extracting the structural calcium metal ions of the protease. Proteases may be affected in product and/or use. Although compositions with high levels of bleach may provide good cleaning, bleach may also impair the performance of enzymes, in particular proteases. This effect is exacerbated by high levels of complexing agent, high temperatures and long cycling times.
Automatic dishwashing compositions may be designed to have optimal performance under certain application conditions, for example the composition may be designed to have optimal performance in a soft water cycle, whereas a composition having optimal performance in a soft water cycle may not have optimal performance in a hard water cycle, or vice versa.
It is an object of the present invention to provide a dishwashing composition which provides improved removal of proteinaceous soils. Preferably, the removal should be good when the composition is used in soft water, and preferably under different water hardness conditions. It is also desirable that the compositions have improved stability and provide improved performance even under stress conditions, such as heavy duty loads of washing in hot long-term cycles.
Disclosure of Invention
According to a first aspect of the present invention, there is provided an automatic dishwashing detergent composition comprising a specific protease. The composition is preferably a phosphate-free automatic dishwashing cleaning composition. More preferably, the composition comprises a complexing agent system, and more preferably the composition comprises a complexing agent system and a high level of a bleaching system. The composition exhibits improved stability and/or performance in terms of egg and/or caramel pudding removal. The compositions of the present invention may be suitable for use in soft water and/or high temperature and/or long cycle times in automatic dishwashing.
According to a second aspect of the invention, there is provided a method of automatic dishwashing using the composition of the invention. Use of a composition according to the invention for providing caramel pudding removal in automatic dishwashing.
The elements of the composition of the invention described in connection with the first aspect of the invention are applicable mutatis mutandis to the other aspects of the invention.
Detailed Description
The present invention encompasses automatic dishwashing cleaning compositions comprising specific proteases. The composition is preferably phosphate-free and preferably comprises a complexing agent system. The compositions achieve improved cleaning under a variety of conditions as compared to cleaning compositions comprising conventional proteases. The composition provides good protein cleaning especially for eggs and caramel pudding soils. The invention also encompasses a method of automatic dishwashing. The compositions of the present invention provide good cleaning when hot, long-circulating and soft water is used.
By "soft" water is meant herein water having a hardness of less than about 2gpg (34.3 ppm). Grains per gallon (gpg) is defined as the unit of water hardness of 1 grain (64.8 mg) of calcium carbonate dissolved in 1US gallon of water (3.785412L). It can be converted to 17.1 parts per million (ppm).
By "thermal" cycle is herein understood a dishwashing program wherein the main cycle is performed at a temperature above 50 ℃, preferably above 55 ℃.
By "long time" cycle is herein understood a dishwashing program wherein the main cycle has a duration of at least 25 minutes, preferably at least 30 minutes and more preferably at least 35 minutes.
The compositions of the invention comprise variant proteases having a defined percentage of identity relative to a reference protease (protease of SEQ ID NO: 1).
The protease of the composition of the invention is sometimes referred to herein as the "protease of the invention". The protease having SEQ ID NO 1 is sometimes referred to herein as a "reference protease".
The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter "sequence identity".
The term "variant" refers to a protease that comprises a mutation (i.e., a substitution, insertion, and/or deletion) at one or more (e.g., several) positions relative to a reference protease. Substitution refers to the replacement of an amino acid occupying a position with another, different amino acid; deletion refers to the removal of an amino acid that occupies a position; and an insertion refers to the addition of an amino acid next to and immediately after the amino acid occupying a position. The variants of the invention have at least 90%, preferably at least 92%, more preferably at least 95% and in particular 99% identity with the reference protease.
The term "wild-type" protease refers to a protease expressed by a naturally occurring microorganism, such as a bacterium, yeast or filamentous fungus that occurs in nature.
In some embodiments, the reference polypeptide comprises bacillus gibsonii-clade subtilisin. In some embodiments, a bacillus gibsonii-clade subtilisin included in a reference polypeptide as provided herein has the amino acid sequence set forth in SEQ ID No. 1.
Enzyme related terms
Nomenclature for amino acid modifications
In describing the enzyme variants herein, the following nomenclature is used for ease of reference: original amino acid position substituted amino acid.
According to this nomenclature, for example, substitution of glycine at position 195 with glutamic acid is shown as G195E. The deletion of glycine at the same position is shown as G195 and the insertion of an additional amino acid residue such as lysine is shown as G195 GK. In case a specific enzyme comprises a "deletion" compared to other enzymes and an insertion is made at this position, this is denoted by 36D to insert an aspartic acid at position 36. Multiple mutations are separated by plus signs, i.e.: S99G + V102N, representing mutations replacing glycine and asparagine with serine and valine at positions 99 and 102, respectively. In case the amino acid at a certain position (e.g. 102) can be substituted by another amino acid selected from a group of amino acids (e.g. the group consisting of N and I), this will be denoted V102N, I.
In all cases, the accepted IUPAC single letter or three letter amino acid abbreviation is employed.
Protease amino acid numbering
The numbering used in this patent is relative to that of SEQ ID NO 1.
Amino acid identity
The relatedness between two amino acid sequences is described by the parameter "identity". For the purposes of the present invention, the alignment of two amino acid sequences is determined by using the Needle program from the EMBOSS software package (http:// EMBOSS. org), version 2.8.0. The Needle program implements the global alignment algorithm described in (1970) J.Mol.biol.48: 443-. The substitution matrix used is BLOSUM62, the gap opening penalty is 10, and the gap extension penalty is 0.5.
The degree of identity between an amino acid sequence of an enzyme as used herein ("the sequence of the invention") and a different amino acid sequence ("the foreign sequence") is calculated as the number of exact matches in an alignment of the two sequences divided by the length of the "sequence of the invention" or the length of the "foreign sequence", whichever is the shortest. Results are expressed as percent identity. Exact matches occur when the "sequences of the invention" and the "foreign sequences" have identical amino acid residues at the same positions of overlap. The length of a sequence is the number of amino acid residues in the sequence.
The terms "succinate based compound" and "succinic acid based compound" are used interchangeably herein.
As used herein, articles such as "a" and "an" when used in a claim are understood to mean one or more of what is claimed or described.
Unless otherwise specified, all components or compositions are on average with respect to the active portion of that component or composition, and do not include impurities, such as residual solvents or by-products, that may be present in commercially available sources of such components or compositions.
All percentages and ratios are by weight unless otherwise indicated. All percentages and ratios are calculated based on the total composition, unless otherwise indicated.
Protease of the invention
The variant of the invention has at least 90%, more preferably at least 92%, more preferably at least 95% and in particular at least 99% identity with the protease of SEQ ID No.1 and comprises one or more amino acid substitutions at one or more positions selected from the group consisting of:
(i)1C/D/E/M/N, 21L, 37A, 54A, 73V, 76D/H/N/T, 83G, 84D/E/F, 85I/M, 86I/S/T/V, 87T, 88M/V, 89F/W, 91I, 95A/N/S, 96M/Q, 97E, 98M, 99A/F/H/I/K/L/Q/T/W/Y, 102L, 104E, 105L, 106I/V, 108A, 109I, 112C, 114M/N, 115A/E/H/Q, 116A/E/G/H/Q, 118A/D/N, 122C, 124E/Q, 126I/Q/V, 128H/I/L/M/N/Q/S/T/V/Y, 129D/H, 130N, 131D/E/N/P/Q, 135A/D/H/K/L/M/N/Q/T/V/W/Y, 138D/E, 139E/L, 141A/E/F/H/Y, 142A/D/E, 143E/H/K/M/S/V, 156E and 157C/D/E
Wherein the amino acid positions of the variants are numbered by correspondence with the amino acid sequence of SEQ ID NO. 1.
Preferably, the variant comprises at least two, more preferably at least three, more preferably at least four amino acid substitutions (numbering using SEQ ID NO:1)
The composition is very advantageous for removing eggs, caramel puddings and/or exhibits good stability. The composition is particularly advantageous for removing caramel pudding.
In respect of caramel pudding removal, preferred compositions comprise a variant having at least 90%, more preferably at least 92%, more preferably at least 95% and especially at least 99% identity to the protease of SEQ ID No.1 and comprising one or more amino acid substitutions at one or more positions selected from the group consisting of: 1D, 21L, 37A, 73V, 76D, 84D/E, 85M, 86I/V, 87T, 96M, 97E, 99A/F/H/I/L/Q/T/W/Y, 104E, 106I/V, 115A/E, 116E, 122C, 126I/Q/V, 128S/T/V, 129D, 130N, 131D/E, 135A/D/H/L/M/N/Q/T/V/W/Y, 138D, 139E/L, 141E/Y, 142D/E, 143E/H/M/V, 156E and 157C/D/E.
The variant has at least 90% identity with the amino acid sequence of SEQ ID NO.1 and preferably comprises at least three substitutions.
Particularly preferred variants for use in the composition of the invention are selected from the group consisting of variants having at least 90%, more preferably at least 92%, more preferably at least 95% and in particular at least 99% identity with the amino acid sequence SEQ ID: 1.
In one embodiment, the composition comprises a protease, wherein the protease is a variant having at least 90% identity to the amino acid sequence of SEQ ID No.1, and the variant comprises one or more amino acid substitutions at one or more positions corresponding to position of SEQ ID No.1, the one or more amino acid substitutions selected from the group consisting of: 1C/D/E/M/N, 21L, 37A, 54A, 73V, 76D/H/N/T, 83G, 84D/E/F, 85I/M, 86I/S/T/V, 87T, 88M/V, 89F/W, 91I, 95A/N/S, 96M/Q, 97E, 98M, 99A/F/H/I/K/L/Q/T/W/Y, 102L, 104E, 105L, 106I/V, 108A, 109I, 112C, 114M/N, 115A/E/H/Q, 116A/E/G/H/Q, 118A/D/N, 122C, 124E/Q, 126I/Q/V, 128H/I/L/M/N/Q/S/T/V/Y, 129D/H, 130N, 131D/E/N/P/Q, 135A/D/H/K/L/M/N/Q/T/V/W/Y, 138D/E, 139E/L, 141A/E/F/H/Y, 142A/D/E, 143E/H/K/M/S/V, 156E and 157C/D/E; wherein the amino acid positions of the variants are numbered by correspondence with the amino acid sequence of SEQ ID NO. 1.
In some embodiments, the variant protease further comprises at least one, two, three or more additional substitutions selected from: Q012E, Q037E, N060D, N097D, Q107E, N115D, N154D, N167D, Q176E, Q185E, Q200E, N205D, Q230E, N236D, N242D, N250D, N253D, Q256D, N253D-Q256D, G025D-M117D-H118D, A149D, R044D-D175D-Y208D-Q230, L041D-G078D-P084D, S101D-T D, I021D-N177D, I021D-S D-T D, I021D-M36122-A D-I021 033672-021D, N021D-I033672-D-I021D-D, N021D-I-36033672-D-I-D-I-021-D-36033672-I-D-I-D-I-36033672-D-I-D-I-D-I-D-I-D-I-, I021V-S039E-N074D-D087E-M122L-N253D, I021V, M122L, M211S, P212N, Q012L, N177V, A222S, V228I, T274N, R099E, N097D-R099E, S097E, S099E, I043E, M122E-N145E-T156E, M211E-P212E, G160E, D127E-M211E-P212E, Q012E-M122E-A222E, D127E, N145E, T E, M E and P36212.
Another embodiment relates to a method of automatic dishwashing, in particular to a method of cleaning caramel pudding stains comprising contacting a surface or article in need of cleaning with a composition comprising a protease, wherein the protease is a variant having at least 90% identity to the amino acid sequence of SEQ ID No.1 and said variant comprises one or more substitutions at one or more positions corresponding to position of SEQ ID No.1 selected from the group consisting of: 1C/D/E/N, 21L, 37A, 73V, 76D/H/N, 84D/E, 85M, 95A, 96M/Q, 97E, 99A/H/Q/T, 104E, 115E, 116E/G, 129D, 131D/E, 135H/K/L/M/Q/T/V/W/Y, 138D, 139E/L, 141E/H/Y, 142D/E, 143E/H/K/M/S/V, 156E, and 157D/E, and combinations; wherein the amino acid positions of the variants are numbered by correspondence with the amino acid sequence of SEQ ID NO. 1.
The protease of the invention performs very well in phosphate-free compositions even when the compositions are used in soft water.
Preferred levels of protease in the compositions of the invention include from about 0.04mg to about 5mg, more preferably from about 0.05mg to about 2mg of active protease per gram of composition.
Automatic dishwashing cleaning composition
The automatic dishwashing cleaning composition may be in any physical form. It may be in the form of a loose powder, a gel or in unit dosage form. Preferably, it is a unit dosage form comprising a compressed tablet and a water-soluble package. The automatic dishwashing cleaning composition of the present invention is preferably present in unit dosage form, and it may be in any physical form, including solid, liquid and gel forms. The composition of the invention is well suited to be present in the form of a multi-compartment package, more particularly in the form of a multi-compartment package comprising compartments for compositions having different physical forms, e.g. one compartment comprises the composition in solid form and another compartment comprises the composition in liquid form. The composition is preferably encapsulated by a water-soluble film such as polyvinyl alcohol. Particularly preferred are compositions in unit dosage form encapsulated in a polyvinyl alcohol film having a thickness of less than 100 μm, preferably from 20 μm to 90 μm. The detergent compositions of the present invention weigh from about 8 grams to about 25 grams, preferably from about 10 grams to about 20 grams. This weight range is well suited to the dispenser of a dishwasher. Although this range is equivalent to a small amount of detergent, the detergent is also formulated in a manner that provides all of the benefits mentioned above.
The composition is preferably phosphate-free. By "phosphate-free" is herein understood that the composition comprises less than 1%, preferably less than 0.1% by weight of the composition of phosphate.
The composition of the present invention is preferably phosphate-free and comprises a complexing agent system.
Complexing agent system
For the purposes of the present invention, a "complexing agent" is a compound capable of binding multivalent ions such as calcium, magnesium, lead, copper, zinc, cadmium, mercury, manganese, iron, aluminum, and other cationic multivalent ions to form a water-soluble complex. The complexing agent has a log stability constant ([ log K ]) for Ca2+ of at least 3. The stability constant log K is determined at a temperature of 25 ℃ in a solution having an ionic strength of 0.1.
The compositions of the present invention comprise from 10% to 50%, by weight of the composition, of a complexing agent system. Preferably, the composition comprises a complexing agent selected from the group consisting of citric acid, methylglycinediacetic acid (MGDA), glutamic acid-N, N-diacetic acid (GLDA), iminodisuccinic acid (IDS), carboxymethyl inulin, L-aspartic acid N, N-diacetic acid tetrasodium salt (ASDA), and mixtures thereof. For the purposes of the present invention, the term "acid" when referring to complexing agents includes acids and salts thereof.
In a preferred embodiment, the composition comprises from 15% to 40% by weight of the composition of the invention of MGDA, more preferably the trisodium salt of MGDA. Compositions containing such high levels of MGDA perform well in the presence of hard water and over long periods of time and/or thermal cycling.
In a preferred embodiment, the composition comprises from 15% to 28% citric acid, more preferably sodium citrate, by weight of the composition of the invention. The composition comprising citric acid performs well in the presence of soft water.
In a preferred embodiment, the complexing agent system comprises citric acid and MGDA in a weight ratio preferably from about 0.5:1 to about 2:1, more preferably from about 0.5:1 to about 2.5: 1.
Dispersant polymers
The dispersant polymer may be used in any suitable amount from about 0.1% to about 20%, preferably from 0.2% to about 15%, more preferably from 0.3% to% by weight of the composition.
The dispersant polymer is capable of suspending calcium or calcium carbonate in an automatic dishwashing process.
The dispersant polymer has a calcium binding capacity in the range of from 30 to 250mg Ca/g dispersant polymer, preferably from 35 to 200mg Ca/g dispersant polymer, more preferably from 40 to 150mg Ca/g dispersant polymer, at 25 ℃. To determine whether a polymer is a dispersant polymer in the sense of the present invention, the following calcium binding capacity assay was performed according to the following description:
calcium binding capacity test method
The calcium binding capacity referred to herein is determined by titration using a pH/ion meter such as a Meettler Toledo SevenMultiTMDesk type counter and PerfectIONTMAnd compounding with calcium ion electrode. To measure binding capacity, a heated agitation device suitable for a beaker or a scrubber tank was set at 25 ℃, and the ion electrode with the meter was calibrated according to the manufacturer's instructions. The standard concentration for electrode calibration should include the test concentration and should be measured at 25 ℃. By mixing 3.67g of CaCl2-2H2O was added to 1L of deionized water to prepare a stock solution of 1000mg/g Ca, which was then diluted to prepare three 100mL each working solutions containing calcium at concentrations of 100mg/g, 10mg/g, and 1mg/g, respectively. 100mg Ca/g working solution was used as initial concentration during titration at 25 ℃. The ionic strength of each working solution was adjusted by adding 2.5g/L NaCl to each working solution. 100mL of 100mg Ca/g working solution was heated and stirred until it reached 25 ℃. An initial reading of the calcium ion concentration was taken using an ion electrode when the solution reached 25 ℃. The test polymer was then added gradually to the calcium working solution (at 0.01g/L intervals) and measured after 5 minutes of stirring after each incremental addition. When the solution reaches 1mg/g calcium, the process is stoppedAnd (4) titrating. The titration process was repeated using the remaining two calcium concentration working solutions. The binding capacity of the test polymer was calculated as the linear slope of the measured calcium concentration relative to grams/L of test polymer added.
The dispersant polymer preferably has a net negative charge when dissolved in an aqueous solution having a pH greater than 6.
The dispersant polymers may also have sulfonated carboxylates or amides to increase the negative charge at lower pH and improve their dispersion characteristics in hard water. Preferred dispersant polymers are sulfonated/carboxylated polymers, i.e., polymers comprising sulfonated monomers and carboxylated monomers.
Preferably, the dispersant polymer is a sulfonated derivative of a polycarboxylic acid and may comprise two, three, four or more different monomer units. Preferred copolymers comprise:
at least one structural unit derived from a carboxylic acid monomer having the general formula (III):
wherein R is1To R3Independently selected from hydrogen, methyl, a linear or branched saturated alkyl group having from 2 to 12 carbon atoms, a linear or branched mono or polyunsaturated alkenyl group having from 2 to 12 carbon atoms, as previously described by-NH 2 or-OH, or-COOH, or COOR4Substituted alkyl or alkenyl groups, wherein R4Selected from hydrogen, alkali metals or linear or branched, saturated or unsaturated alkyl or alkenyl groups having 2 to 12 carbons;
preferred carboxylic acid monomers include one or more of the following: acrylic acid, maleic anhydride, itaconic acid, citraconic acid, 2-phenylacrylic acid, cinnamic acid, crotonic acid, fumaric acid, methacrylic acid, 2-ethylacrylic acid, methylenemalonic acid, or sorbic acid. Acrylic acid and methacrylic acid are more preferred.
Optionally, one or more structural units derived from at least one nonionic monomer having the general formula (IV):
wherein R is5To R7Independently selected from hydrogen, methyl, phenyl or hydroxyalkyl groups containing from 1 to 6 carbon atoms and may be part of a cyclic structure, X is selected from-CH2-, -COO-, -CONH-or-CONR-8Optionally present spacer groups of (A) and R8Selected from linear or branched, saturated alkyl groups having from 1 to 22 carbon atoms or unsaturated, preferably aromatic, groups having from 6 to 22 carbon atoms.
Preferred nonionic monomers include one or more of the following: butene, isobutene, pentene, 2-methylpent-1-ene, 3-methylpent-1-ene, 2,4, 4-trimethylpent-2-ene, cyclopentene, methylcyclopentene, 2-methyl-3-methylcyclopentene, hexene, 2, 3-dimethylhex-1-ene, 2, 4-dimethylhex-1-ene, 2, 5-dimethylhex-1-ene, 3, 5-dimethylhex-1-ene, 4, 4-dimethylhex-1-ene, cyclohexene, methylcyclohexene, cycloheptene, alpha-olefins having 10 or more carbon atoms such as dec-1-ene, dec-2-methylpent-1-ene, and the like, Dodec-1-ene, hexadec-1-ene, octadec-1-ene and docec-1-ene, and preferred aromatic monomers are styrene, alpha-methylstyrene, 3-methylstyrene, 4-dodecylstyrene, 2-ethyl-4-benzylstyrene, 4-cyclohexylstyrene, 4-propylstyrene, 1-vinylnaphthalene, 2-vinylnaphthalene; preferred carboxylic ester monomers are methyl (meth) acrylate, ethyl (meth) acrylate, propyl (meth) acrylate, t-butyl (meth) acrylate, pentyl (meth) acrylate, hexyl (meth) acrylate, 2-ethylhexyl (meth) acrylate, octyl (meth) acrylate, lauryl (meth) acrylate, stearyl (meth) acrylate, and behenyl (meth) acrylate; preferred amides are N-methylacrylamide, N-ethylacrylamide, N-tert-butylacrylamide, N-2-ethylhexylacrylamide, N-octylacrylamide, N-laurylacrylamide, N-stearylacrylamide, N-behenylacrylamide.
And at least one structural unit derived from at least one sulfonic acid monomer having the general formulae (V) and (VI):
wherein R is7Is a group comprising at least one sp2 bond, a is O, N, P, S, an amide group, or an ester bond, B is a monocyclic or polycyclic aromatic or aliphatic group, each t is independently 0 or 1, and M + is a cation. In one aspect, R7Are C2 to C6 olefins. In another aspect, R7 is ethylene, butene, or propylene.
Preferred sulfonated monomers include one or more of the following: 1-acrylamido-1-propanesulfonic acid, 2-acrylamido-2-methyl-1-propanesulfonic acid, 2-methacrylamido-2-methyl-1-propanesulfonic acid, 3-methacrylamido-2-hydroxy-propanesulfonic acid, allylsulfonic acid, methallylsulfonic acid, allyloxybenzenesulfonic acid, methallyloxybenzenesulfonic acid, 2-hydroxy-3- (2-propenyloxy) propanesulfonic acid, 2-methyl-2-propene-1-sulfonic acid, styrenesulfonic acid, vinylsulfonic acid, 3-sulfopropyl methacrylate, sulfomethacrylamide, sulfomethylmethacrylamide, and mixtures of the acids mentioned or their water-soluble salts.
Preferably, the polymer comprises the following amounts of monomers: from about 40% to about 90%, preferably from about 60% to about 90%, by weight of the polymer, of one or more carboxylic acid monomers; from about 5% to about 50%, preferably from about 10% to about 40%, by weight of the polymer, of one or more sulfonic acid monomers; and optionally from about 1% to about 30%, preferably from about 2% to about 20%, by weight of the polymer, of one or more nonionic monomers. Particularly preferred polymers comprise from about 70% to about 80% by weight of the polymer of at least one carboxylic acid monomer, and from about 20% to about 30% by weight of the polymer of at least one sulfonic acid monomer.
In the polymer, all or some of the carboxylic acid groups or sulfonic acid groups may be present in neutralized form, i.e. the acidic hydrogen atoms of the carboxylic acid groups and/or sulfonic acid groups in some or all of the acid groups may be replaced with metal ions, preferably alkali metal ions, and in particular with sodium ions.
The carboxylic acid is preferably (meth) acrylic acid. The sulfonic acid monomer is preferably 2-acrylamido-2-propanesulfonic Acid (AMPS).
Preferred commercially available polymers include: alcosperse 240, Aquacreat AR 540, and Aquacreat MPS supplied by Alco Chemical; acumer 3100, Acumer 2000, Acusol 587G, and Acusol 588G, supplied by Rohm & Haas; goodrich K-798, K-775, and K-797 supplied by BF Goodrich; and ACP 1042 provided by ISP technologies Inc. Particularly preferred polymers are Acusol 587G and Acusol 588G supplied by Rohm & Haas.
Suitable dispersant polymers include low molecular weight anionic carboxylic acid polymers. They may be homopolymers or copolymers having a weight average molecular weight of less than or equal to about 200000g/mol, or less than or equal to about 75000g/mol, or less than or equal to about 50000g/mol, or from about 3000g/mol to about 50000g/mol, preferably from about 5000g/mol to about 45000 g/mol. The dispersant polymer may be a low molecular weight homopolymer of polyacrylate having an average molecular weight of 1000 to 20000, specifically 2000 to 10000, and specifically preferably 3000 to 5000.
The dispersant polymer may be a copolymer of acrylic acid and methacrylic acid having a molecular weight of less than 70000, a copolymer of acrylic acid and/or methacrylic acid and maleic acid, and a copolymer of acrylic acid and/or methacrylic acid and fumaric acid. Its molecular weight is in the range of 2000 to 80000g/mol and more preferably 20000 to 50000g/mol and in particular 30000 to 40000g/mol and the ratio of (meth) acrylate to maleate or fumarate segments is 30:1 to 1: 2.
The dispersant polymer may be a copolymer of acrylamide and an acrylate having a molecular weight of 3000 to 100000 or 4000 to 20000, and an acrylamide content of less than 50% or less than 20% by weight of the dispersant polymer may also be used. Alternatively, such dispersant polymers may have a molecular weight of 4000 to 20000 and an acrylamide content of 0% to 15% by weight of the polymer.
Dispersant polymers suitable for use herein also include itaconic acid homopolymers and copolymers.
Alternatively, the dispersant polymer may be selected from the group consisting of alkoxylated polyalkyleneimines, alkoxylated polycarboxylates, polyethylene glycols, styrene copolymers, cellulose sulfates, carboxylated polysaccharides, amphiphilic graft copolymers, and mixtures thereof.
Bleaching system
The compositions of the present invention comprise a bleaching system comprising a high level of bleach, preferably percarbonate, in combination with a bleach activator or bleach catalyst or both. Preferably, the bleach activator is TAED and the bleach catalyst is a manganese bleach catalyst.
Bleaching agent
The compositions of the present invention preferably comprise from about 10% to about 20%, more preferably from about 12% to about 18%, by weight of the composition, of a bleaching agent, preferably percarbonate.
Inorganic bleaching agents and organic bleaching agents are suitable for use herein. Inorganic bleaching agents include perhydrate salts such as perborate, percarbonate, perphosphate, persulfate and persilicate salts. The inorganic perhydrate salts are normally the alkali metal salts. The inorganic perhydrate salt may be included as the crystalline solid without additional protection. Alternatively, the salt may be coated. Suitable coatings include sodium sulfate, sodium carbonate, sodium silicate and mixtures thereof. The coating may be applied as a mixture to a surface or sequentially as layers.
Alkali metal percarbonates, in particular sodium percarbonate, are preferred bleaching agents for use herein. The percarbonate is most preferably incorporated into the product in a coated form, which provides stability within the product.
Potassium peroxymonopersulfate is another inorganic perhydrate salt useful herein.
Typical organic bleaching agents are organic peroxyacids, especially dodecanediperoxy acid, tetradecanediperoxy acid and hexadecanediperoxy acid. Monoperazelaic acid and dipelargonac acid, monopercridecanoic acid and dipelargonac acid are also suitable for use herein. Diacyl and tetraacyl peroxides such as dibenzoyl peroxide and dilauroyl peroxide are other organic peroxides that may be used in the context of the present invention.
Other typical organic bleaching agents include peroxyacids, specific examples being alkyl peroxyacids and aryl peroxyacids. Preferred representatives are (a) perbenzoic acids and ring-substituted derivatives thereof, such as alkylperoxybenzoic acids, and magnesium peroxy-alpha-naphthoate and monoperphthalate, (b) aliphatic or substituted aliphatic peroxyacids such as peroxylauric acid, peroxystearic acid, epsilon-phthalimidoperoxycaproic acid [ Phthalimidoperoxycaproic Acid (PAP) ], phthalimidoperoxycaproic acid, N-nonenamidoadipic acid and N-nonenamidosulfosuccinate, and (c) aliphatic and araliphatic peroxydicarboxylic acids, such as 1, 12-diperoxycarboxylic acid, 1, 9-diperoxyazelaic acid, diperoxydecanedioic acid, diperoxyphthalic acid, 2-decylderoxybutane-1, 4-dioic acid, N-terephthaloylbis (6-aminoperoxycaproic acid).
Bleach activators
Bleach activators are typically organic peracid precursors that enhance bleaching during cleaning at temperatures of 60 c and below. Bleach activators suitable for use herein include compounds which provide aliphatic peroxycarboxylic acids, preferably having from 1 to 12 carbon atoms, specifically from 2 to 10 carbon atoms, and/or optionally substituted perbenzoic acid under perhydrolysis conditions. Suitable materials have O-acyl and/or N-acyl groups and/or optionally substituted benzoyl groups with the indicated number of carbon atoms. Preference is given to the use of a polyacylated alkylenediamine, in particular Tetraacetylethylenediamine (TAED), acylated triazine derivatives, in particular 1, 5-diacetyl-2, 4-dioxohexahydro-1, 3, 5-triazine (DADHT), acylated glycolurils, in particular Tetraacetylglycoluril (TAGU), N-acylimides, in particular N-Nonanoylsuccinimide (NOSI), acylated phenolsulfonates, in particular N-nonanoyl or isononanoyloxybenzenesulfonates (N-or iso-NOBS), decanoyloxybenzoic acid (DOBA), carboxylic anhydrides, in particular phthalic anhydride, acylated polyols, in particular triacetin, ethylene glycol diacetate and 2, 5-diacetoxy-2, 5-dihydrofuran, and acetyl triethyl citrate (TEAC). If present, the compositions of the present invention comprise from 0.01% to 5%, preferably from 0.2% to 2%, by weight of the composition, of a bleach activator, preferably TAED.
Bleaching catalyst
The compositions herein preferably comprise a bleach catalyst, preferably a metal-containing bleach catalyst. More preferably, the metal-containing bleach catalyst is a transition metal-containing bleach catalyst, especially a manganese-or cobalt-containing bleach catalyst.
Preferred bleach catalysts for use herein include manganese triazacyclononane and related complexes; co, Cu, Mn and Fe bipyridinamines and related complexes; and pentaamineacetic acid cobalt (III) and related complexes. Particularly preferred bleach catalysts for use herein are 1,4, 7-trimethyl-1, 4, 7-triazacyclononane (Me-TACN) and 1,2,4, 7-tetramethyl-1, 4, 7-triazacyclononane (Me/Me-TACN).
Preferably, the composition of the present invention comprises from 0.001% to 0.5%, more preferably from 0.002% to 0.05%, more preferably from 0.005% to 0.075% of bleach catalyst by weight of the composition. Preferably, the bleach catalyst is a manganese bleach catalyst.
Inorganic builder
The compositions of the present invention preferably comprise inorganic builders. Suitable inorganic builders are selected from the group consisting of carbonates, silicates and mixtures thereof. Particularly preferred for use herein is sodium carbonate. Preferably, the composition of the invention comprises from 5% to 60%, more preferably from 10% to 50% and in particular from 15% to 45% of sodium carbonate by weight of the composition.
Surface active agent
Surfactants suitable for use herein include nonionic surfactants, preferably the composition is free of any other surfactant. Traditionally, nonionic surfactants have been used for surface modification purposes in automatic dishwashing, in particular for sheeting, to avoid filming and spotting and to improve gloss. It has been found that nonionic surfactants can also help prevent soil redeposition.
Preferably, the composition of the invention comprises a non-ionic surfactant or a non-ionic surfactant system, more preferably said non-ionic surfactant or non-ionic surfactant system has a phase inversion temperature of between 40 ℃ and 70 ℃, preferably between 45 ℃ and 65 ℃, as measured in distilled water at a concentration of 1%. By "nonionic surfactant system" is meant herein a mixture of two or more nonionic surfactants. Preferred for use herein are nonionic surfactant systems. The nonionic surfactant system appears to have improved cleaning and conditioning characteristics and better stability in the product compared to the single nonionic surfactant.
The phase inversion temperature is the temperature below which the surfactant or mixture thereof preferentially partitions into the aqueous phase as oil-swollen micelles, and above which the surfactant partitions into the oil phase as water-swollen reversed micelles. The phase inversion temperature can be determined visually by identifying at which temperature cloudiness occurs.
The phase inversion temperature of the nonionic surfactant or system can be determined as follows: a solution was prepared comprising 1% of the corresponding surfactant or mixture in distilled water by weight of the solution. The solution was gently stirred prior to phase inversion temperature analysis to ensure that the process occurred at chemical equilibrium. The phase transition temperature was obtained in a heat stable bath by dipping the solution in a 75mm sealed glass test tube. To ensure that there was no leakage, the test tubes were weighed before and after the phase inversion temperature measurement. The temperature is gradually increased at a rate of less than 1 c/minute until the temperature reaches a few degrees below the estimated phase transition temperature. The phase inversion temperature was determined visually under the first turbidity mark.
Suitable nonionic surfactants include: i) ethoxylated nonionic surfactants prepared by reaction of a monohydric alkanol or alkylphenol having 6 to 20 carbon atoms with preferably at least 12 moles, especially preferably at least 16 moles and still more preferably at least 20 moles of ethylene oxide per mole of alcohol or alkylphenol; ii) an alcohol alkoxylated surfactant having 6 to 20 carbon atoms and at least one ethoxy and propoxy groups. Preferred for use herein are mixtures of surfactants i) and ii).
Another suitable nonionic surfactant is an epoxy-terminated poly (alkoxylated) alcohol represented by the formula:
R1O[CH2CH(CH3)O]x[CH2CH2O]y[CH2CH(OH)R2] (I)
wherein R1 is a straight or branched chain aliphatic hydrocarbon group having 4 to 18 carbon atoms; r2 is a straight or branched chain aliphatic hydrocarbon group having 2 to 26 carbon atoms; x is an integer having an average value of 0.5 to 1.5, more preferably about 1; and y is an integer having a value of at least 15, more preferably at least 20.
Preferably, the surfactant of formula I is terminated with an epoxide unit [ CH2CH (OH) R2%]Having at least about 10 carbon atoms. According to the present invention, a suitable surfactant of formula I is that of Olin CorporationSLF-18B nonionic surfactant, as described in WO 94/22800 published by Olin Corporation, 10, 13, 1994.
Enzyme
Other proteases
In addition to the protease of the invention, the composition of the invention may also comprise a protease. Mixtures of two or more proteases can help enhance cleaning over a wide range of temperatures, cycle durations, and/or substrates, and provide excellent shine benefits, especially when used in combination with anti-redeposition agents and/or sulfonated polymers.
Suitable proteases for use in combination with the variant proteases of the invention include metalloproteases and serine proteases including neutral or alkaline microbial serine proteases such as subtilisin (EC 3.4.21.62). Suitable proteases include those of animal, plant or microbial origin. In one aspect, such suitable proteases may be of microbial origin. Suitable proteases include chemically modified or genetically modified mutants of the aforementioned suitable proteases. In one aspect, suitable proteases may be serine proteases, such as alkaline microbial proteases or/and trypsin-type proteases. Examples of suitable neutral or alkaline proteases include:
(a) subtilisins (EC 3.4.21.62), in particular those of WO2004067737, WO2015091989, WO2015091990, WO2015024739, WO2015143360, US 6,312,936B1, US 5,679,630, US 4,760,025, DE102006022216a1, DE102006022224a1, WO2015089447, WO2015089441, WO2016066756, WO2016066757, WO2016069557, WO2016069563, the sources described in WO2016069569 (such as bacillus, bacillus lentus (b.lentus), bacillus alcalophilus (b.alkalophilus), bacillus subtilis (b.subtilis), bacillus amyloliquefaciens (b.amyloliquefaciens), bacillus pumilus (b.pumilus), bacillus gibsonii (b.gibbsii) and bacillus oklaensis (b.akibaiii)).
(b) Trypsin-type or chymotrypsin-type proteases, such as trypsin (e.g. porcine-or bovine-derived trypsin), including the fusarium protease described in WO 89/06270 and chymotrypsin derived from the genus cellulomonas (Cellumonas) described in WO 05/052161 and WO 05/052146.
(c) Metalloproteinases, in particular those described in WO 07/044993a2, which are derived from bacillus amyloliquefaciens; those derived from bacillus, Brevibacillus (Brevibacillus), Thermoactinomyces (Thermoactinomyces), bacillus (Geobacillus), Paenibacillus (Paenibacillus), lysine bacillus (Lysinibacillus) or streptomyces species described in WO2014194032, WO2014194054 and WO 2014194117; those described in WO2015193488 from kribbella allergen; and those described in WO2016075078 which are derived from streptomyces and Lysobacter (Lysobacter).
(d) Proteases having at least 90% identity to the subtilases described in WO92/17577(Novozymes A/S) from Bacillus TY145, NCIMB 40339, including variants of this Bacillus TY145 subtilase described in WO2015024739 and WO 2016066757.
Particularly preferred proteases for use in the detergents of the invention are polypeptides having at least 90%, preferably at least 95%, more preferably at least 98%, even more preferably at least 99%, in particular 100% identity with the wild-type enzyme from bacillus lentus, which polypeptides comprise mutations at one or more, preferably two or more, more preferably three or more of the following positions, using the BPN' numbering system and amino acid abbreviations as shown in WO00/37627 (which is incorporated herein by reference): V68A, N76D, N87S, S99D, S99SD, S99A, S101G, S101M, S103A, V104N/I, G118V, G118R, S128L, P129Q, S130A, Y167A, R170S, A194P, V205I, Q206L/D/E, Y209 36209 209W and/or M222S.
Most preferably, the protease is selected from the group comprising the following mutations (BPN' numbering system) relative to the PB92 wild type (SEQ ID NO:2 in WO 08/010925) or subtilisin 309 wild type (sequence according to PB92 backbone, except comprising the natural variation N87S).
(i)G118V+S128L+P129Q+S130A
(ii)S101M+G118V+S128L+P129Q+S130A
(iii)N76D+N87R+G118R+S128L+P129Q+S130A+S188D+N248R
(iv)N76D+N87R+G118R+S128L+P129Q+S130A+S188D+V244R
(v)N76D+N87R+G118R+S128L+P129Q+S130A
(vi)V68A+N87S+S101G+V104N
(vii)S99AD
Suitable commercially available additional proteases include those under the trade name LiquanaseSavinase BlazeAndthose sold by Novozymes A/S (Denmark); under the trade name of PurafectPurafect And PurafectThose sold by Dupont; under the trade name ofAndthose sold by Solvay Enzymes; and available from Henkel/KemiraThose of (i.e., BLAP) (the sequence shown in figure 29 of US 5,352,604, with the following mutations S99D + S101R + S103A + V104I + G159S, hereinafter referred to as BLAP), BLAP R (BLAP with S3T + V4I + V199M + V205I + L217D), BLAP X (BLAP with S3T + V4I + V205I), and BLAP F49 (BLAP with S3T + V4I + a194P + V199M + V205I + L217D); and KAP from Kao (alkalophilic bacillus subtilisin with mutations a230V + S256G + S259N).
Particularly preferred for use herein in combination with the variant proteases of the invention are commercial proteases selected from the group consisting of: BlazeBLAP and BLAP variants.
Preferred levels of protease enzyme in the products of the invention include from about 0.05mg to about 10mg, more preferably from about 0.5mg to about 7mg and especially from about 1mg to about 6mg of active protease enzyme per gram of composition.
Amylase
Preferably, the composition of the invention may comprise an amylase. Suitable alpha-amylases include those of bacterial or fungal origin. Chemically or genetically modified mutants (variants) are included. Preferred alkaline alpha-amylases are derived from strains of Bacillus (Bacillus), such as Bacillus licheniformis (Bacillus licheniformis), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus stearothermophilus (Bacillus stearothermophilus), Bacillus subtilis (Bacillus subtilis), or other Bacillus (Bacillus sp.), such as Bacillus nc122bi 89, NCBI 12512, NCBI 12513, DSM 9375(USP 7,153,818) DSM 12368, DSMZ number 12649, KSM AP1378(WO 97/00324), KSM K36, or KSM K38(EP 1,022,334). Preferred amylases include:
(a) variants described in WO 96/23873, WO00/60060, WO06/002643 and WO2017/192657, in particular variants having one or more substitutions in the following positions with respect to SEQ ID No. 11:
26. 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 202, 214, 231, 246, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 461, 471, 482, 484, these variants preferably further comprising D and G184 deletions.
(b) Variants exhibiting at least 90% identity to SEQ ID No.4 in WO06/002643, the wild-type enzyme from bacillus SP722, in particular variants having deletions at positions 183 and 184, and the variants described in WO00/60060, WO2011/100410 and WO2013/003659, which are incorporated herein by reference.
(c) Variants exhibiting at least 95% identity to the wild-type enzyme from Bacillus 707 (SEQ ID NO:7 in US 6,093,562), in particular those comprising one or more of the following mutations: m202, M208, S255, R172, and/or M261. Preferably, the amylase comprises one or more of M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N, and/or R172Q. Particularly preferred are those comprising the M202L or M202T mutations.
(d) The variants described in WO 09/149130, preferably those which show at least 90% identity with SEQ ID NO:1 or SEQ ID NO:2 in WO 09/149130 (wild-type enzyme from Bacillus stearothermophilus (Geobacillus Stearothermophilus) or a truncated version thereof).
(e) Variants exhibiting at least 89% identity with SEQ ID No.1 in WO2016091688, in particular those comprising a deletion at position H183+ G184 and further comprising one or more mutations at position 405, 421, 422 and/or 428.
(f) A variant exhibiting at least 60% amino acid sequence identity with "PcuAmyl alpha-amylase" from Paenibacillus coagulans YK9 (SEQ ID NO:3 in WO 2014099523).
(g) A variant exhibiting at least 60% amino acid sequence identity to the "CspAmy 2 amylase" from Cytophaga sp (SEQ ID NO:1 in WO 2014164777).
(h) Variants exhibiting at least 85% identity to AmyE from Bacillus subtilis (SEQ ID NO:1 in WO 2009149271).
(i) A variant exhibiting at least 90% identity to a wild-type amylase from bacillus KSM-K38 (accession No. AB 051102).
(j) Variants exhibiting at least 80% identity to the mature amino acid sequence of AAI10 from Bacillus (SEQ ID NO:7 in WO 2016180748).
(k) Variants exhibiting at least 80% identity to the mature amino acid sequence of a Alicyclobacillus (Alicyclobacillus sp.) amylase (SEQ ID NO:8 in WO 2016180748).
Preferably, the amylase is an engineered enzyme in which one or more of the bleach-oxidizing amino acids have been substituted with a less oxidizing amino acid. In particular, it is preferred that the methionine residue is substituted by any other amino acid. In particular, it is preferred that the methionine most susceptible to oxidation is substituted. Preferably, the methionine at the position equivalent to 202 in SEQ ID NO 11 is substituted. Preferably, the methionine at this position is substituted by threonine or leucine, preferably leucine.
Suitable commercially available alpha-amylases include TERMAMYL STAINZYME And(Novozymes A/S,Bagsvaerd,Denmark)、AT 9000 Biozym Biotech Trading GmbH Wehlistrasse 27b A-1200 Wien Austria、OPTISIZE PREFERENZ series (including PREFERENZ)And PREFERENZ)、PURASTAR (DuPont., Palo Alto, California) and(Kao,14-10 Nihonbashi Kayabacho,1-chome, Chuo-ku Tokyo 103-8210, Japan). In one aspect, suitable amylases include And STAINZYMEAnd mixtures thereof.
Preferably, the product of the invention comprises at least 0.01mg, preferably from about 0.05mg to about 10mg, more preferably from about 0.1mg to about 6mg, especially from about 0.2mg to about 5mg of active amylase per g of composition.
Preferably, the protease and/or amylase of the composition of the invention is in the form of granules comprising more than 29% sodium sulphate by weight of the granules, and/or the weight ratio of sodium sulphate to active enzyme (protease and/or amylase) is between 3:1 and 100:1, or preferably between 4:1 and 30:1, or more preferably between 5:1 and 20: 1.
Crystal growth inhibitor
Crystal growth inhibitors are materials that can bind to calcium carbonate crystals and prevent further growth of substances such as aragonite and calcite.
Examples of effective crystal growth inhibitors include phosphonates, polyphosphonates, inulin derivatives, polyitaconic acid homopolymers, and cyclic polycarboxylates.
Suitable crystal growth inhibitors may be selected from HEDP (1-hydroxyethylidene 1, 1-diphosphonic acid), carboxymethyl inulin (CMI), tricarboxylic acids and cyclic carboxylates. For the purposes of the present invention, the term carboxylate encompasses both the anionic form and the protonated carboxylic acid form.
The cyclic carboxylate comprises at least two, preferably three or preferably at least four carboxylate groups and the cyclic structure is based on a mono-or bi-cyclic alkane or heterocycle. Suitable cyclic structures include cyclopropane, cyclobutane, cyclohexane or cyclopentane or cycloheptane, bicycloheptane or bicyclooctane and/or tetrahydrofuran. One preferred crystal growth inhibitor is cyclopentane tetracarboxylate.
Cyclic carboxylates having at least 75%, preferably 100%, carboxylate groups on the same side of the 3D structure of the ring or in the "cis" position are preferred for use herein.
Preferably, the two carboxylate groups located on the same side of the ring are in directly adjacent or "ortho" positions.
Preferred crystal growth inhibitors include HEDP, tricarboxylic acids, tetrahydrofuran tetracarboxylic acid (THFTCA), and cyclopentane tetracarboxylic acid (CPTCA). THFTCA is preferably in the 2c,3t,4t,5 c-configuration and CPTCA in the cis, cis-configuration. A particularly preferred crystal growth inhibitor for use herein is HEDP.
Also preferred for use herein are partially decarboxylated polyitaconic acid homopolymers, preferably having a decarboxylation level in the range of 50 to 90 mole%. A particularly preferred polymer for use herein is Itaconix, supplied by Itaconix
The amount of crystal growth inhibitor is preferably from about 0.01% to about 10%, specifically from about 0.02% to about 5%, and specifically from 0.05% to 3%, by weight of the composition.
Metal care agent
Metal conditioners can prevent or reduce tarnishing, corrosion or oxidation of metals, including aluminum, stainless steel and non-ferrous metals such as silver and copper. Preferably, the composition of the invention comprises from 0.1% to 5%, more preferably from 0.2% to 4% and especially from 0.3% to 3% by weight of the product of a metal care agent, preferably the metal care agent is Benzotriazole (BTA).
Glass nursing agent
The glass care agent protects the appearance of the glass article during dishwashing. Preferably, the composition of the present invention comprises 0.1% by weight of the compositionTo 5%, more preferably 0.2% to 4% and especially 0.3% to 3% of a metal care agent, preferably the glass care agent is a zinc-containing material, especially hydrozincite. Other suitable glass care agents are Polyethyleneimines (PEI). Particularly preferred PEI is that provided by BASFFG。
The automatic dishwashing composition of the present invention preferably has a pH of from about 9 to about 12, more preferably from about 10 to less than about 11.5 and especially from about 10.5 to about 11.5, measured as a 1% w/v aqueous solution in distilled water at 20 ℃.
The automatic dishwashing composition of the present invention preferably has a reserve alkalinity of from about 10 to about 20, more preferably from about 12 to about 18, at a pH of 9.5, as measured in NaOH at 100 grams of product at 20 ℃.
Preferred automatic dishwashing compositions of the present invention comprise:
i) from 10% to 20% by weight of the composition of sodium percarbonate;
ii) from 10% to 50% by weight of the composition of an organic complexing agent system, preferably said complexing agent system comprises MGDA;
iii)TAED;
iv) an amylase;
v) optionally but preferably from 5% to 50% by weight of the composition of an inorganic builder, preferably sodium carbonate;
vi) optionally but preferably from 2% to 10% by weight of the composition of a nonionic surfactant;
vii) other optional ingredients, including: crystal growth inhibitors (preferably HEDP) and glass care agents.
Preferred automatic dishwashing compositions of the present invention comprise:
i) from 10% to 20% by weight of the composition of a bleaching agent, preferably sodium percarbonate;
ii) from 10% to 50% by weight of the composition of an organic complexing agent system.
iii) a manganese bleach catalyst and optionally TAED;
iv) an amylase;
v) optionally but preferably from 5% to 50% by weight of the composition of an inorganic builder, preferably sodium carbonate;
vi) optionally but preferably from 2% to 10% by weight of the composition of a nonionic surfactant;
vii) optional but preferred glass care agents.
Example 1
The compositions shown in table 1 were prepared. 3g of each composition was dissolved in one liter of deionized water to produce a cleaning solution having a pH of 11. The protease of the invention is added to the cleaning solution at a level between 0.25ppm and 0.75 ppm.
Table 1: automatic dishwashing composition
Table 1: automatic Dishwashing (ADW) compositions
The composition provides good caramel pudding removal.
Example 2
Cleaning performance in detergents
Stain of egg yolk
Egg yolk (PAS-38, Center for Tes) was used relative to a reference protease (having the amino acid sequence SEQ ID NO:1)TMMaterials BV, Vlardingen, Netherlands) micro-swatches and GSM-B detergents (see Table 1 below) the automation of testing protease variants described herein in microtiter plates (MTP) at pH 10.5Dishwashing (ADW) cleaning performance. In this assay, the pre-perforated PAS-38 (to fit in the MTP) was used for both washed and unwashed samples. Rinsed samples were prepared by adding 180. mu.L of 10mM CAPS buffer (pH 11) to MTP containing PAS-38 micro-samples and shaking at 60 ℃ and 1100rpm for 30 minutes. After incubation, the buffer was removed, the sample was rinsed with deionized water to remove any residual buffer, and the MTP was air dried before use in the assay. All micro-sample plates were filled with 3g/l of GSM-B detergent adjusted to 374ppm water hardness before enzyme addition.
Alternatively, the cleaning performance of the variants on egg yolk stains was tested by using melamine tiles of DM21 prepared from CFT (Vlaardingen, the Netherlands) as described herein. Egg yolk on melamine tiles was prepared from CFT using 2.5g material/tile.
Melamine tiles were used as caps and pressed tightly against microtiter plates (MTP). DM21 tiles were pre-rinsed at 40 ℃ with shaking at 250rpm for 40 minutes using 300. mu.L of 10mM CAPS pH 11 (containing 0.005% Tween-80). The tile was rinsed with tap water and dried overnight. For cleaning, 3g/L of GSMB (see table 1) was adjusted to 374ppm water hardness and each enzyme sample was added to the MTP before attaching the melamine tile lid to the MTP. The volume capacity of the MTP, and thus the volume of solution added thereto, can vary, wherein a minimum volume of solution should be added to the MTP that enables contact between the solution and the stained surface. In this example, a volume of 300 μ Ι _ of detergent containing enzyme was added to each well of an aluminum 96-well MTP. The MTP was incubated in an Infors thermal shaker at 40 ℃ for 45 minutes at 250 rpm. After incubation, the tiles were removed from the MTP and air dried.
Stain removal was quantified by taking a picture of the plate and measuring the RGB values for each stained area using custom software. The percent soil removal (% SRI) value of the washed tile was calculated by applying the RGB values to the following formula:
%SRI=(ΔE/ΔEinitial)*100
Wherein Δ E ═ SQR ((R)Rear end-RFront side)2+(GRear end-GFront side)2+(BBack-front)2)
Wherein Δ EInitial=SQR((RWhite colour (Bai)-RFront side)2+(GWhite colour (Bai)-GFront side)2+(BWhite-anterior)2)
Cleaning performance was obtained by subtracting the value of the blank control (no enzyme) from each sample value (hereinafter referred to as "blank-subtracted cleaning"). For each condition and variant, the Performance Index (PI) was calculated by dividing the blank subtracted clean by the blank subtracted clean of the reference protease (having the amino acid sequence SEQ ID NO:1) at the same concentration. The value of the reference protease PI was determined from a standard curve of the reference protease, which was included in the test and fitted to Langmuir fit (Langmuir fit).
Caramel pudding stain
Caramel pudding stain: the cleaning performance of the variants on caramel pudding stain was tested by using a custom made melamine dish washer monitor (ceramic tile) manufactured by CFT, Vlaardingen, Netherlands and labeled DM10c as described herein. The DM10c tiles used in this study were prepared using 2.7g of the same material as used to prepare the commercially available DM10 monitor (caramel pudding deboc. com product), but baked at 140 ℃ for 2 hours instead of 150 ℃.
AAPF Activity assay
The protease activity of the reference protease and variants thereof was tested by measuring the hydrolysis of N-suc-AAPF-pNA. The reagent solutions used for the AAPF hydrolysis assay were: 100mM Tris/HCl pH 8.6, which contained 0.005%(Tris dilution buffer); 100mM Tris buffer pH 8.6, containing 10mM CaCl2And 0.005%(Tris/Ca buffer); and 160mM suc-AAPF-pNA (suc-AAPF-pNA stock solution) (Sigma: S-7388) in DMSO. A substrate working solution was prepared by adding 1mL of suc-AAPF-pNA stock solution to 100mL of Tris/Ca buffer and mixing well. The enzyme samples were added to MTP (Greiner 781101) containing 1mg/suc-AAPF-pNA working solution and the activity was determined in kinetic mode at 405nm for 3 min at Room Temperature (RT) using a SpectraMax plate reader. The absorbance of the blank without protease was subtracted from each sample reading. Protease activity in mOD min-1And (4) showing.
Stability determination
The stability of the variants described herein was measured by diluting the variants in stress buffer and measuring the proteolytic activity of the variants using the AAPF assay described above before and after the heat incubation step. The temperature and duration of the heat incubation step are selected such that the reference protease shows about 50% residual activity. Stability was measured under Tris-EDTA (50mM Tris pH 9; 5mM EDTA; 0.005% Tween 80) buffer. The stable PI is obtained by dividing the residual activity of the variant by the residual activity of the reference protease.
Example 3
Properties of the variants
The cleaning benefits of the reference protease and variants thereof prepared according to example 2 were evaluated in the following assay described in example 2: PAS-38 or DM21 clean in GSM-B detergent, caramel pudding (DM10c) clean in GSM-B (Table 1) or MGDA (Table 2) detergent, and stability in Tris/EDTA. The results of these evaluations of the reference protease and variants thereof are reported as Performance Index (PI) values calculated relative to the reference protease as described in example 2 and are shown in table 3. ND means that the value is not determined or that the value is outside the confidence interval of the determination.
Denotes PI values obtained from measurements performed using DM21 ceramic tiles
The following variants showed an improved performance index (PI value ≧ 1.1) compared to the reference protease on one of the following assays (PAS-38 (washed or unwashed) or caramel pudding (GSMB or MGDA detergent) ADW clean, or stability in Tris-EDTA buffer): Q001/D/E/M/021, T037, T054, L073, S076/H/N/083, P084/E/085/086/S/T/087, L088/089/091, G095/N/096/097, G098, R099/F/H/I/K/L/Q/T/W/102, G104, I105, A106/107, G108, L109, A112, Q114/M/115/E/H/116/E/G/H/117, H118/D/122, L124/126/Q/T/128/I/L/M/N/Q/S/T/V/Y, P129D/H, S130N, S131D/E/N/P/Q/T, R135A/D/H/K/L/M/N/Q/T/V/W/Y, N138D/E, Y139E/L, T141A/E/F/H/Y, S142A/D/E, R143A/E/H/K/M/Q/S/V, N154D, S156E, G157C/D/E, M211D/E/N/Q, M216Q
In at least one PAS-38 assay, the following variants showed improved ADW cleaning performance index (PI value ≧ 1.1) compared to the reference protease: P084F, S085I, A086I/S/T/V, Y089F, V091I, G108A, L109I, A112C, Q114A/M/N, N115A, N116Q, M117I, H118N, M122C, A126I/Q/T/V, G128T/V, S130N, S131N, Y139E/L, T141 39141 141A/E/Y, S142A, N154D, M211D/E/N/Q
In at least one caramel pudding assay, the following variants showed improved ADW cleaning performance index (PI value ≧ 1.1) compared to the reference protease: Q001C/D/E/N, I021L, T037A, L073V, S076D/H/N, P084/084D/E, S085M, G095A, A096M/Q, N097E, R099A/H/Q T, G104E, Q107E, N115E, N116E/E129E, S131E/E135E/K/L/M/Q/T/V/W/E138E, Y139E/E141E/H/E142/E143E/E/H/K/M/Q/S/E, S156E, G157E/36211/N/Q/W/E
The following variants showed improved stability in Tris-EDTA buffer compared to the reference protease (PI value ≧ 1.1): T054A, A083G, P084F, A086V, D087T, L088M, Y089W, G095A/N/S, N097E, G098E, R099E/F/H/I/K/L/Q/T/W/E102E, I105E, A106E/E, G108E, Q114E/E, H118E/E, L124E/E126/Q/T/E128/I/L/M/N/Q/S/T/V/E/E/N/P/Q/36135/D/H/K/M/N/Q/S/T/V/E/36138, and L088/E/36138, R143A/E/H/K/M/Q/S/V, N154D, S156E, G157C/D/E, M211D, M216Q
The following variants show improved performance indices (PI value ≧ 1.1) in at least one PAS-38 assay or in at least one caramel pudding assay compared to the reference protease, and these variants show improved stability in Tris-EDTA buffer: p084, A086, G095, N097, R099/H/Q/107, G108, Q114/115/116, M122, A126/Q/T/128/129, S131/E/135/K/L/M/Q/T/V/W/138, Y139, T141/142/143/E/H/K/M/Q/S/154, S156, G157/211
In at least one PAS-38 or caramel pudding assay, the following variants showed improved performance index (PI value ≧ 1.1) and reduced net charge compared to the reference protease: Q001C/D/E, S07D, P084D/E, N097E, R099A/H/Q/T, G104E, Q107E, N115E, N116E, P129E, S131E/E135E/K/L/M/Q/T/V/W/E138, Y139E, T141E/E142/E143E/E/H/K/M/Q/S/E, S156E, G157E/36211E
The following variants showed improved stability (PI value ≧ 1.1) and reduced net charge in Tris-EDTA buffer compared to the reference protease: N097E, R099A/F/H/I/K/L/Q/T/W/Y, Q107E, N115E, N116E, H118D, M122C, L124E, G128Y, P129D, S131D/E, R135A/D/H/K/L/M/N/Q/T/V/W/Y, N138/138D/E, Y139E, T141E, S142D/E, R143A/E/H/K/M/Q/S/V, N154D, S156E, G157C/D/E, M211D
The dimensions and values disclosed herein are not to be understood as being strictly limited to the exact numerical values recited. Rather, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40 mm" is intended to mean "about 40 mm".
Claims (15)
1. An automatic dishwashing cleaning composition comprising a protease, wherein said protease is a variant having at least 90% identity to the amino acid sequence of SEQ ID No.1, and said variant comprises one or more amino acid substitutions at one or more positions corresponding to position of SEQ ID No.1, said one or more amino acid substitutions being selected from the group consisting of:
(i)1C/D/E/M/N, 21L, 37A, 54A, 73V, 76D/H/N/T, 83G, 84D/E/F, 85I/M, 86I/S/T/V, 87T, 88M/V, 89F/W, 91I, 95A/N/S, 96M/Q, 97E, 98M, 99A/F/H/I/K/L/Q/T/W/Y, 102L, 104E, 105L, 106I/V, 108A, 109I, 112C, 114M/N, 115A/E/H/Q, 116A/E/G/H/Q, 118A/D/N, 122C, 124E/Q, 126I/Q/V, 128H/I/L/M/N/Q/S/T/V/Y, 129D/H, 130N, 131D/E/N/P/Q, 135A/D/H/K/L/M/N/Q/T/V/W/Y, 138D/E, 139E/L, 141A/E/F/H/Y, 142A/D/E, 143E/H/K/M/S/V, 156E and 157C/D/E
Wherein the amino acid positions of the variants are numbered by correspondence with the amino acid sequence of SEQ ID NO. 1.
2. The composition of claim 1, wherein the variant comprises one or more amino acid substitutions at one or more positions corresponding to position 1 of SEQ ID NO, said one or more amino acid substitutions selected from the group consisting of: 1D; 21L; 37A; 73V; 76D; 84D/E; 85M; 86I/V; 87T; 96M; 97E; 99A/F/H/I/L/Q/T/W/Y; 104E, performing a chemical reaction; 106I/V; 115A/E; 116E; 122C, and (3); 126I/Q/V; 128S/T/V; 129D; 130N; 131D/E; 135A/D/H/L/M/N/Q/T/V/W/Y; 138D; 139E/L; 141E/Y; 142D/E; 143E/H/M/V; 156E; and 157C/D/E.
3. The composition of any one of claims 1 or 2, wherein the protease is a variant having at least 95% identity to the amino acid sequence of SEQ ID No. 1.
4. The composition of any one of the preceding claims, wherein the composition is phosphate-free.
5. The composition of any preceding claim, wherein the composition comprises from 10% to 50%, by weight of the composition, of an organic complexing agent system.
6. A composition according to any preceding claim comprising more than 10% by weight of the composition of a bleaching agent.
7. Composition according to the preceding claim, comprising a bleach activator and/or bleach catalyst, preferably a manganese catalyst.
8. The composition according to any one of claims 5 to 7, wherein the complexing agent system comprises a complexing agent selected from the group consisting of citric acid, methylglycinediacetic acid, glutamic-N, N-diacetic acid, iminodisuccinic acid, carboxymethyl inulin, salts thereof and mixtures thereof, preferably a salt of methylglycinediacetic acid.
9. The composition of any of claims 5 to 8 wherein the complexing agent system comprises citric acid and methylglycinediacetic acid, preferably in a weight ratio of about 0.5:1 to about 2: 1.
10. The composition of any one of claims 5 to 9, wherein the bleaching agent is percarbonate.
11. The composition according to any one of the preceding claims, wherein the composition further comprises an alpha amylase, preferably having a mutation at a position equivalent to 202 in SEQ ID No. 2.
12. The composition according to any one of the preceding claims, wherein the composition comprises a dispersant polymer, preferably a carboxylated/sulfonated polymer.
13. The composition of any one of the preceding claims, comprising:
i) from 10% to 50%, by weight of the composition, of an organic complexing agent system;
ii) a bleaching system comprising at least 10% by weight of the composition of percarbonate and optionally a bleach activator and/or bleach catalyst;
iii) a nonionic surfactant;
iv) a dispersant polymer;
v) an amylase; and
vi) optionally but preferably a glass care agent.
14. A method of washing soiled dishes in a dishwashing machine, said method comprising the steps of:
i) providing the soiled dishes;
ii) treating the dishes with a cleaning composition according to any of the preceding claims; and
iii) optionally rinsing the dishes.
15. Use of the composition of any one of claims 1 to 12 for removing caramel pudding in automatic dishwashing.
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US201862686833P | 2018-06-19 | 2018-06-19 | |
US62/686,833 | 2018-06-19 | ||
US201962852321P | 2019-05-24 | 2019-05-24 | |
US62/852,321 | 2019-05-24 | ||
PCT/US2019/036889 WO2019245839A1 (en) | 2018-06-19 | 2019-06-13 | Automatic dishwashing detergent composition |
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US (1) | US11220656B2 (en) |
EP (1) | EP3810769A1 (en) |
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