CN112200452A - Disinfection effect evaluation method - Google Patents
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- CN112200452A CN112200452A CN202011073310.8A CN202011073310A CN112200452A CN 112200452 A CN112200452 A CN 112200452A CN 202011073310 A CN202011073310 A CN 202011073310A CN 112200452 A CN112200452 A CN 112200452A
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Abstract
The invention discloses a disinfection effect evaluation method, which comprises the following steps: acquiring a first image of the surface of an object to be evaluated before disinfection; sterilizing the surface of the object to be evaluated to obtain a first image of the surface of the object to be evaluated before sterilization; sterilizing the surface of the object to be evaluated; acquiring a second image of the surface of the object to be evaluated after disinfection; detecting the simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values; and analyzing and processing the first image, the second image and the set of test values to establish a disinfection effect evaluation model. The disinfection effect evaluation method establishes a practical equipment poisoning surface disinfection training effect evaluation method system, realizes semi-quantitative and comprehensive evaluation on the equipment disinfection training effect of chemical toxic and harmful substances, performs entity training, and provides a technical platform for standard disinfection training and effect inspection of a novel disinfection technology.
Description
Technical Field
The invention relates to the technical field of disinfection treatment, in particular to a disinfection effect evaluation method.
Background
HD, dichloroethylsulfide (2,2 '-dichlorodiethylsulfide, β, β' -dichlorodiethylsulfide, bis (2-chloroethyl) sulfide), is volatile, is mainly used in organic synthesis, drugs (useful for treating certain hyperproliferative diseases) and manufacturing of military toxic hazardous substances, and is notorious for its widespread use in toxic hazardous substances.
At present, there are many methods for detecting HD, which mainly include: the method comprises the following steps of reacting HD hydrocarbon property or compound formation property with a chemical reagent, and detecting by a colorimetric method, wherein common detection reagents comprise a thymolphthalein (blue reagent) method, a C- (4-nitrobenzyl) pyridine method (DB-3 method), a sodium nitrosoferricyanide method, a thiourea-nickel salt reagent method and the like, and the detection method has the problems that a detected medicine is easy to lose efficacy and has weak anti-interference capability; the two laboratory instrument analysis methods are to detect HD original shapes and hydrolysis products thiodiglycol thereof by using a gas chromatography-mass spectrometer (GC-MS) and to detect residual trace HD and the like in soil by using a gas chromatography flame photometer. Therefore, the research and development of a novel effective rapid and sensitive detection and analysis method for toxic and harmful substances and related compounds is still an important subject, and particularly, aiming at the current situation that the equipment disinfection training effect evaluation technology is weak, a practical equipment contamination surface disinfection training effect evaluation method system is urgently needed to be established, so that the quantitative and comprehensive evaluation on the equipment disinfection training effect of chemical toxic and harmful substances is realized.
Disclosure of Invention
The invention aims to provide a disinfection effect evaluation system, which is used for solving the problem that a practical disinfection training effect evaluation method for an equipment contaminated surface is lacked in the prior art.
In order to achieve the above object, the present invention provides a sterilization effect evaluation method, comprising:
acquiring a first image of the surface of an object to be evaluated before disinfection;
sterilizing the surface of the object to be evaluated;
acquiring a second image of the surface of the object to be evaluated after disinfection;
detecting the simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values;
and analyzing and processing the first image, the second image and the set of test values to establish a disinfection effect evaluation model.
Preferably, the step of detecting the simulated toxic and harmful substances remaining on the surface of the object to be evaluated to obtain a set of test values includes:
pumping the simulated toxic and harmful substances remained on the surface of the object to be evaluated into a detector;
heating the simulated toxic and harmful substances in the detector;
acquiring the temperature of the simulated toxic and harmful substances in the detector;
and if the acquired temperature of the simulated toxic and harmful substances reaches a preset temperature threshold, detecting the simulated toxic and harmful substances in the detector to obtain the set of test values.
Preferably, the step of analyzing the first image, the second image and the set of test values to establish a disinfection effectiveness evaluation model comprises:
converting the set of test values to a set of toxic or hazardous substance concentrations;
processing the first image and the second image to obtain a pixel color change curve of a contaminated area of the surface of the object to be evaluated in the first image and the second image;
obtaining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to the concentration of the toxic and harmful substances and the color change curve of the pixel;
and establishing a disinfection effect evaluation model according to the corresponding relation between the concentration of the toxic and harmful substances and the pixel color.
Preferably, the test value is a resistance signal, and the resistance signal is converted into the concentration of the toxic and harmful substances according to the principle of the gas sensor.
Preferably, the step of processing the first image and the second image to obtain a pixel color variation curve of an infected area on the surface of the object to be evaluated in the first image and the second image includes:
preprocessing the first image and the second image;
determining the infected area of the surface of the object to be evaluated in the first image and the second image;
respectively extracting color values of all pixel points of the contaminated areas in the first image and the second image;
and obtaining a pixel color change curve of the contaminated area on the surface of the object to be evaluated according to the color values of all the pixel points of the contaminated area in the first image and the second image.
Preferably, the step of obtaining the corresponding relationship between the toxic and harmful substance concentration and the pixel color according to the set of toxic and harmful substance concentrations and the pixel color variation curve includes:
establishing a toxic and harmful substance concentration change curve according to the group of toxic and harmful substance concentrations;
and determining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to the concentration change curve of the toxic and harmful substances and the color change curve of the pixel.
Preferably, the step of establishing a disinfection effect evaluation model according to the correspondence between the toxic and harmful substance concentration and the pixel color includes:
processing the corresponding relation between the concentration of the toxic and harmful substances and the pixel color by using a least square method curve fitting algorithm to obtain a disinfection effect evaluation model;
the disinfection effect evaluation model comprises two evaluation indexes, namely a toxic and harmful substance concentration value and a pixel change rate.
Preferably, the step of acquiring a first image of the surface of the object to be evaluated before sterilization further comprises:
and smearing the simulated toxic and harmful substances on the surface of the object to be evaluated.
Preferably, the simulated toxic and harmful substance is a mixed solution of dichloroethyl sulfide and a luminol reagent.
Preferably, the surface of the object to be evaluated is provided with a plurality of circular metal sheets, and the simulated toxic and harmful substances are smeared on the circular metal sheets.
The invention has the following advantages:
the disinfection effect evaluation method comprises the steps of obtaining a first image and a second image of the surface of an object to be evaluated before and after disinfection, detecting simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values, analyzing and processing the first image, the second image and the group of test values to establish a disinfection effect evaluation model, establishing a practical equipment contamination surface disinfection training effect evaluation method system by utilizing a method combining experimental research and actual training based on a toxic and harmful substance detection technology and a computer image analysis and processing technology, realizing semi-quantitative and comprehensive evaluation on the equipment disinfection training effect of chemical toxic and harmful substances, performing entity training, and providing a technical platform for standard disinfection training and effect inspection of a novel disinfection technology.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.
Drawings
FIG. 1 is a schematic flow diagram illustrating a disinfection effectiveness assessment method according to an exemplary embodiment;
FIG. 2 is a flow chart diagram illustrating a step 400 of a disinfection effectiveness assessment method according to an exemplary embodiment;
FIG. 3 is a flow chart diagram illustrating a step 500 of a disinfection effectiveness assessment method according to an exemplary embodiment;
FIG. 4 is a flow chart diagram illustrating step 502 of a disinfection effectiveness assessment method according to an exemplary embodiment;
fig. 5 is a diagram illustrating a sterilization effect evaluation model according to an exemplary embodiment.
Detailed Description
The technical solutions of the present invention will be clearly and completely described below in connection with specific embodiments, but it should be understood by those skilled in the art that the embodiments described below are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and substitutions may be made by those skilled in the art without departing from the spirit and scope of the invention, and all such modifications and substitutions are intended to be within the scope of the claims.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
The invention is further described with reference to the following figures and examples:
to achieve the above object, referring to fig. 1, an embodiment of the present invention provides a sterilization effect evaluation method, including:
step 100: acquiring a first image of the surface of an object to be evaluated before disinfection;
step 200: sterilizing the surface of an object to be evaluated;
step 300: acquiring a second image of the surface of the disinfected object to be evaluated;
step 400: detecting the simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values;
step 500: and analyzing and processing the first image, the second image and a group of test values to establish a disinfection effect evaluation model.
It should be noted that, in step 100 and step 300 of this embodiment, an image of a surface of an object to be evaluated may be acquired by a camera, in step 200, a sterilization process may be performed on the object to be evaluated by a sterilization apparatus, where the object to be evaluated may be a device or an instrument, in step 400, a simulated toxic and harmful substance remaining on the surface of the object to be evaluated may be detected by a detector, and in step 500, a sterilization effect model may be established by a portable mobile terminal.
The disinfection effect evaluation method provided by the embodiment of the invention comprises the steps of obtaining a first image and a second image of the surface of an object to be evaluated before and after disinfection, detecting simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values, and then analyzing and processing the first image, the second image and the group of test values to establish a disinfection effect evaluation model.
According to the above scheme, further referring to fig. 2, the step 400 of the disinfection effect evaluation method of the embodiment of the present invention includes:
step 401: pumping the simulated toxic and harmful substances on the surface of the object to be evaluated into a detector;
step 402: heating the simulated toxic and harmful substances in the detector;
the accessible adds the hotting mask and heats the poisonous and harmful substance of simulation in this step, and heatable arrives indoor temperature, for example: 15-20 ℃.
Step 403: acquiring the temperature of the toxic and harmful substance simulated in the detector;
in the step, the temperature of the toxic and harmful substances can be detected and simulated by the temperature sensor.
Step 404: and if the acquired temperature of the simulated toxic and harmful substances reaches a preset temperature threshold, detecting the simulated toxic and harmful substances in the detector to obtain a group of test values.
In the step, the detected temperature of the simulated toxic and harmful substances is judged, and the simulated toxic and harmful substances are detected by the gas sensor when the temperature value reaches a preset temperature threshold, wherein the preset temperature threshold can be 15 ℃, 16 ℃, 17 ℃, 18 ℃, 19 ℃ or 20 ℃, and is determined according to actual needs and is not specifically limited herein.
According to the above solution, further, referring to fig. 3, the step 500 of the disinfection effect evaluation method according to the embodiment of the present invention includes:
step 501: converting a test value into the concentration of the toxic and harmful substances;
step 502: processing the first image and the second image to obtain a pixel color change curve of a contaminated area of the surface of the object to be evaluated in the first image and the second image;
step 503: obtaining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to a group of concentration of the toxic and harmful substances and a pixel color change curve;
step 504: and establishing a disinfection effect evaluation model according to the corresponding relation between the concentration of the toxic and harmful substances and the pixel color.
The disinfection effect evaluation method provided by the embodiment of the invention is used for establishing a practical disinfection training effect evaluation model of the infected area based on a toxic and harmful substance detection technology and a computer image analysis processing technology, and the establishment of the disinfection effect evaluation model needs to research the corresponding relation and the change rule between the concentration of the simulated toxic and harmful substance and the concentration of the simulated toxic and harmful substance before and after disinfection and the pixel color of the image of the infected area.
According to the scheme, further, the test value in the disinfection effect evaluation method is the resistance signal, and the resistance signal is converted into the concentration of the toxic and harmful substances according to the principle of the gas sensor.
According to the above solution, further, referring to fig. 4, step 502 of the disinfection effect evaluation method according to the embodiment of the present invention includes:
step 5021: preprocessing the first image and the second image;
the image preprocessing in the step comprises the following steps: filtering, noise reduction, white balance, etc.
Step 5022: determining a contaminated area of the surface of the object to be evaluated in the first image and the second image;
in this step, the contaminated area may be determined according to the color change of the image, and the first image and the image may be segmented.
Step 5023: respectively extracting color values of all pixel points of the contaminated areas in the first image and the second image;
step 5024: and obtaining a pixel color change curve of the contaminated area on the surface of the object to be evaluated according to the color values of all the pixel points of the contaminated area in the first image and the second image.
According to the above scheme, further, step 503 of the disinfection effect evaluation method of the embodiment of the present invention includes:
step 5031: establishing a toxic and harmful substance concentration change curve according to a group of toxic and harmful substance concentrations;
step 5032: and determining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to the concentration change curve of the toxic and harmful substances and the color change curve of the pixel.
According to the above scheme, further, step 504 of the disinfection effect evaluation method of the embodiment of the invention comprises:
processing the corresponding relation between the concentration of the toxic and harmful substances and the pixel color by using a least square method curve fitting algorithm to obtain a disinfection effect evaluation model;
the disinfection effect evaluation model comprises two evaluation indexes, namely a toxic and harmful substance concentration value and a pixel change rate.
See the disinfection effectiveness evaluation model shown in fig. 5: where the amount of toxic or biochemical substance used is fixed, e.g., 1L, x represents the detection value and the curve represents the final fitted curve effect.
The disinfection effect evaluation method provided by the embodiment of the invention is characterized in that a data dispersion curve simulating the concentration of toxic and harmful substances and the pixel color is established, the dispersion points of the curve are subjected to binomial fitting through a least square principle curve fitting algorithm, an equation set is solved, the fitted curve is optimally approximated to known data, and the optimal and proper curve is found out, wherein the curve is a disinfection effect evaluation model (mathematical model), and evaluation indexes are two parameters (namely a concentration value and a pixel change rate) of the mathematical model.
According to the above solution, further, as shown in fig. 1, step 100 of the disinfection effect evaluation method according to the embodiment of the present invention further includes:
step 100 a: and smearing the simulated toxic and harmful substances on the surface of the object to be evaluated.
According to the scheme, the simulated toxic and harmful substances in the disinfection effect evaluation method are mixed liquid of dichloroethyl sulfide and a luminol reagent.
According to the scheme, further, the surface of the object to be evaluated is provided with a plurality of circular metal sheets, and the simulated toxic and harmful substances are smeared on the circular metal sheets.
In summary, in the disinfection effect evaluation method of the embodiment of the present invention, the simulated toxic and harmful substances are coated on the surface of the round metal sheet on the surface of the evaluated object, the image before disinfection is photographed by the image pickup device to disinfect the surface of the evaluated object, the residual toxic and harmful substances on the surface of the round metal sheet are detected by the detector after disinfection to obtain a group of test values, and the test results are transmitted to the portable terminal through bluetooth; and acquiring the disinfected picture through the camera device, and transmitting the image before disinfection and the disinfected image to the portable terminal through the USB interface. The portable terminal analyzes and processes the plurality of groups of test values and the plurality of groups of images to establish an evaluation model and an evaluation index of the disinfection effect.
Claims (10)
1. A sterilization effect evaluation method, comprising:
acquiring a first image of the surface of an object to be evaluated before disinfection;
sterilizing the surface of the object to be evaluated;
acquiring a second image of the surface of the object to be evaluated after disinfection;
detecting the simulated toxic and harmful substances remained on the surface of the object to be evaluated to obtain a group of test values;
and analyzing and processing the first image, the second image and the set of test values to establish a disinfection effect evaluation model.
2. A sterilization effect evaluation method according to claim 1, wherein said step of detecting the simulated toxic and harmful substance remaining on the surface of the object to be evaluated to obtain a set of test values comprises:
pumping the simulated toxic and harmful substances remained on the surface of the object to be evaluated into a detector;
heating the simulated toxic and harmful substances in the detector;
acquiring the temperature of the simulated toxic and harmful substances in the detector;
and if the acquired temperature of the simulated toxic and harmful substances reaches a preset temperature threshold, detecting the simulated toxic and harmful substances in the detector to obtain the set of test values.
3. The disinfection effectiveness evaluation method according to claim 1, wherein the step of analyzing the first image, the second image, and the set of test values to create a disinfection effectiveness evaluation model comprises:
converting the set of test values to a set of toxic or hazardous substance concentrations;
processing the first image and the second image to obtain a pixel color change curve of a contaminated area of the surface of the object to be evaluated in the first image and the second image;
obtaining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to the concentration of the toxic and harmful substances and the color change curve of the pixel;
and establishing a disinfection effect evaluation model according to the corresponding relation between the concentration of the toxic and harmful substances and the pixel color.
4. The disinfection effect evaluation method according to claim 3, wherein the test value is a resistance signal, and the resistance signal is converted into a toxic harmful substance concentration according to a gas sensor principle.
5. A sterilization effect evaluation method according to claim 3, wherein the step of processing the first image and the second image to obtain a pixel color variation curve of an affected area of the surface of the object to be evaluated in the first image and the second image comprises:
preprocessing the first image and the second image;
determining the infected area of the surface of the object to be evaluated in the first image and the second image;
respectively extracting color values of all pixel points of the contaminated areas in the first image and the second image;
and obtaining a pixel color change curve of the contaminated area on the surface of the object to be evaluated according to the color values of all the pixel points of the contaminated area in the first image and the second image.
6. The disinfection effect evaluation method according to claim 3, wherein the step of obtaining the correspondence between the toxic and harmful substance concentration and the pixel color from the set of toxic and harmful substance concentrations and the pixel color variation curve includes:
establishing a toxic and harmful substance concentration change curve according to the group of toxic and harmful substance concentrations;
and determining the corresponding relation between the concentration of the toxic and harmful substances and the color of the pixel according to the concentration change curve of the toxic and harmful substances and the color change curve of the pixel.
7. The disinfection effect evaluation method according to claim 3, wherein the step of establishing a disinfection effect evaluation model based on the correspondence between the toxic and harmful substance concentration and the pixel color comprises:
processing the corresponding relation between the concentration of the toxic and harmful substances and the pixel color by using a least square method curve fitting algorithm to obtain a disinfection effect evaluation model;
the disinfection effect evaluation model comprises two evaluation indexes, namely a toxic and harmful substance concentration value and a pixel change rate.
8. The sterilization effect evaluation method according to claim 1, wherein the step of acquiring the first image of the surface of the object to be evaluated before sterilization is further preceded by:
and smearing the simulated toxic and harmful substances on the surface of the object to be evaluated.
9. The disinfection effect evaluation method of claim 8, wherein the simulated toxic and harmful substance is a mixture of dichloroethyl sulfide and luminol reagent.
10. The disinfection effect evaluation method according to claim 8, wherein the surface of the object to be evaluated is provided with a plurality of circular metal pieces on which the simulated toxic and harmful substance is smeared.
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