CN112198649A - Sample pool placing table for light sheet fluorescence microscope - Google Patents
Sample pool placing table for light sheet fluorescence microscope Download PDFInfo
- Publication number
- CN112198649A CN112198649A CN202011159875.8A CN202011159875A CN112198649A CN 112198649 A CN112198649 A CN 112198649A CN 202011159875 A CN202011159875 A CN 202011159875A CN 112198649 A CN112198649 A CN 112198649A
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- China
- Prior art keywords
- placing table
- placing
- fluorescence microscope
- sample cell
- sample
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Abstract
A sample cell placing table for a light sheet fluorescence microscope is disclosed, wherein the sample cell can be cuvettes with different sizes. One end of the placing table provided by the invention is provided with a threaded hole, and the size of the threaded hole is matched with that of the optical platform; the groove is arranged in the middle of the placing table, so that the weight of the placing table is reduced, and the mechanical stability is improved; the other end of the placing table is provided with an L-shaped side wall and a base, wherein the middle of the L-shaped side wall is provided with a threaded hole for fixing cuvettes with different sizes. The placing table is completed by three-dimensional printing, and the three-dimensional printing material is resin. The invention is specially designed for a light-sheet fluorescence microscope using a cuvette as a sample pool, and the placing table can move on an optical platform through a three-dimensional displacement platform so as to meet the placing requirements of biological samples with different sizes and shapes in actual experiment operation.
Description
Technical Field
The invention belongs to the field of instrument design, mainly relates to a light-sheet microscope system, and particularly relates to a special placing table of a light-sheet microscope, which is suitable for a cuvette serving as a sample pool.
Background
The light sheet microscope has important value and significance in the imaging of living bodies and scanning bodies of organisms. In fluorescence microscopy of living organisms, sample preparation and container placement are important, since this affects not only the normal growth and development process of the organism, but also the imaging resolution of light sheet microscopy. For a common inverted microscope, a sample is placed on a glass slide, then the glass slide is placed on an object stage to be subjected to water immersion or oil immersion and the like, and then a microscope objective with a proper magnification is selected to observe and photograph. However, the light sheet microscope is different from a common microscope in that the illumination light path is perpendicular to the detection light path, so that the sample is placed differently. Therefore, different sample placing containers need to be designed for different biological samples in consideration of the optical properties of the microscope itself and the biological characteristics of the sample itself. The placing platform designed by the invention can enable the cuvette sample pool to be stably positioned on the working planes of the two microscope objectives and to be freely moved in a three-dimensional space.
Disclosure of Invention
The invention aims to solve the problem of sample placement in a light-sheet microscope and provides a special placing table of the light-sheet microscope suitable for a cuvette. For a common inverted microscope, a sample is placed on a glass slide, then the glass slide is placed on an object stage to be subjected to water immersion or oil immersion and the like, and then a microscope objective with a proper magnification is selected to observe and photograph. However, unlike conventional microscopes, the illumination path is perpendicular to the detection path, so that the sample can be placed directly on the slide. Therefore, for different biological samples, combining the optical properties of the microscope itself and the biological characteristics of the sample itself, there are currently three commonly used sample cells: round capillaries, square capillaries and cuvettes. Circular capillaries are less expensive, but cause refraction of the beam due to the circular wall. When fluorescence excitation is performed using laser light, a circular capillary tube has a non-negligible effect on both illumination quality and imaging quality. Square capillaries circumvent this problem, but are expensive and allow limited sample volumes. For fluorescence imaging of larger biological samples, therefore, four-sided transparent cuvettes are typically used as sample containers.
When the cuvette is adopted as the sample cell, the problem of how to adjust the target area of the biological sample to the imaging focus needs to be considered, and the problem of how to ensure the stability of the sample cell in the imaging process needs to be considered. The placing table provided by the invention is specially designed for the cuvette sample pool, can carry cuvettes with various sizes, and can ensure that the function of an optical sheet fluorescence microscope system is not influenced. In practical use, the sample cell needs to be combined with a displacement platform to move on an optical platform for precise control. The placing table can meet the requirements of placing and precisely controlling biological samples with different sizes and different shapes in the light-sheet fluorescence microscope.
The invention has the advantages and positive effects that:
in the light sheet microscope, the illumination light path is perpendicular to the detection light path, and the use of the cuvette has the advantages of eliminating Rayleigh scattering, improving the imaging quality of the light sheet microscope and performing volume imaging on a biological sample.
The groove is arranged in the middle of the placing table, so that the weight of the placing table can be reduced, the placing table is more stable, and the subsequent dynamic imaging experiment of the biological sample is facilitated.
The middle of the base for placing the sample cell in the placing table is empty, so that observation of a detection microscope objective in a light-sheet microscope is facilitated. Even if a microscope objective with high magnification and short focal length is used, the focusing process of the microscope objective can not be obstructed by the placing platform. The invention is specially designed for a cuvette sample pool, an L-shaped side wall is designed at one end of a placing table, a threaded hole is formed in the middle of the side wall, and cuvettes with different sizes can be fixed by screwing matched screws into the threaded hole in the side wall of the placing table.
The material of the placing table in the invention is resin, so that the placing table is convenient to process and can be used for 3D printing.
The invention belongs to the technical field of optics and photoelectricity, can be used in light sheet microscope imaging, and has important potential application value in a plurality of fields of living body biological dynamic imaging with low phototoxicity, rapidness and high resolution, micro-nano particle control and the like.
Drawings
Fig. 1 is a three-dimensional view of the structure of the placing table.
Fig. 2 is a schematic view of the working principle of the placing table.
Fig. 3 is a photograph of the real object after the placement table is combined with the cuvette sample cell. Wherein (a) is a top view of the placing table, (b) is a left side view of the placing table, and (c) is a front view of the placing table.
Fig. 4 is a real object photographing picture after the placing table and the three-dimensional displacement table are assembled.
Detailed Description
The invention provides a placing table for placing a cuvette sample cell in a light-sheet fluorescence microscope, which is characterized in that 6M 6 threaded holes are designed at one end of the placing table shown in figure 1, and the size of each threaded hole is matched with that of an optical platform.
The middle of the base of the placing table provided by the invention is hollow, so that a detection microscope objective in a light-sheet microscope can conveniently observe a biological sample in a contrast cuvette, and the working principle of the placing table is shown in figure 2.
The invention provides a placing table, wherein an L-shaped side wall is designed at one end of the placing table, a cuvette is placed on a base, the cuvette is roughly fixed by utilizing the side wall of a cuboid, and a threaded hole is formed in the middle of the L-shaped side wall. The M4 screw is screwed into the threaded hole on the side wall of the placing table, so that the cuvette can be suitable for cuvettes with different sizes, and the assembled effect is shown in figure 3.
The placing table provided by the invention can be combined with the three-dimensional displacement table through the screw hole at the bottom, and the position of the cuvette can be three-dimensionally adjusted by utilizing the displacement table, so that different positions of a sample are imaged. The effect of the combination of the placement stage and the three-dimensional displacement stage is shown in fig. 4.
Claims (6)
1. The utility model provides a platform is placed to sample cell for slide fluorescence microscope which characterized in that: one end of the placing table is provided with a threaded hole, and the size of the threaded hole is matched with that of the optical platform; the middle of the placing table is provided with a groove so as to reduce the weight of the placing table; the other end of the placing table is provided with an L-shaped side wall and a base, and the middle of one side wall is provided with a threaded hole for fixing cuvettes with different sizes.
2. The placing table for placing a sample cell in a light sheet fluorescence microscope according to claim 1, characterized in that: the placing table is made of resin materials.
3. The placing table for placing a sample cell in a light sheet fluorescence microscope according to claim 1, characterized in that: the middle of the placing table is provided with a groove so as to reduce the weight of the placing table and ensure that the placing table is more stable.
4. The placing table for placing a sample cell in a light sheet fluorescence microscope according to claim 1, characterized in that: the middle of a base for placing the sample cell in the placing table is empty, so that focusing of a detection microscope objective in the light-sheet microscope on a biological sample in the contrast color vessel during observation cannot be influenced.
5. The placing table for placing a sample cell in a light sheet fluorescence microscope according to claim 1, characterized in that: one end of the placing table is provided with an L-shaped side wall for keeping the cuvette stable.
6. The placing table for placing the sample cell in the light sheet fluorescence microscope according to claim 5, wherein: the matching screws are screwed into the threaded holes in the side wall of the placing table, so that cuvettes with different sizes can be fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011159875.8A CN112198649A (en) | 2020-10-27 | 2020-10-27 | Sample pool placing table for light sheet fluorescence microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011159875.8A CN112198649A (en) | 2020-10-27 | 2020-10-27 | Sample pool placing table for light sheet fluorescence microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112198649A true CN112198649A (en) | 2021-01-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011159875.8A Pending CN112198649A (en) | 2020-10-27 | 2020-10-27 | Sample pool placing table for light sheet fluorescence microscope |
Country Status (1)
| Country | Link |
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| CN (1) | CN112198649A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105607240A (en) * | 2016-01-21 | 2016-05-25 | 核工业理化工程研究院 | Base for microscope |
| CN106053346A (en) * | 2016-06-29 | 2016-10-26 | 华中科技大学 | Light sheet microscopic imaging conversion apparatus |
| US20180088308A1 (en) * | 2016-09-28 | 2018-03-29 | University Of Washington | Inverted light-sheet microscope |
| CN207976384U (en) * | 2017-11-13 | 2018-10-16 | 重庆三峡学院 | A kind of multi-functional Transmissivity measurement holder |
| WO2019011658A1 (en) * | 2017-07-11 | 2019-01-17 | Carl Zeiss Microscopy Gmbh | Adapter for use with a sample holder, and method for arranging a sample in a detection beam path of a microscope |
-
2020
- 2020-10-27 CN CN202011159875.8A patent/CN112198649A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105607240A (en) * | 2016-01-21 | 2016-05-25 | 核工业理化工程研究院 | Base for microscope |
| CN106053346A (en) * | 2016-06-29 | 2016-10-26 | 华中科技大学 | Light sheet microscopic imaging conversion apparatus |
| US20180088308A1 (en) * | 2016-09-28 | 2018-03-29 | University Of Washington | Inverted light-sheet microscope |
| WO2019011658A1 (en) * | 2017-07-11 | 2019-01-17 | Carl Zeiss Microscopy Gmbh | Adapter for use with a sample holder, and method for arranging a sample in a detection beam path of a microscope |
| CN207976384U (en) * | 2017-11-13 | 2018-10-16 | 重庆三峡学院 | A kind of multi-functional Transmissivity measurement holder |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210108 |
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| WD01 | Invention patent application deemed withdrawn after publication |