CN112194331A - Biological inducer for removing bottom mud pollutants of black and odorous riverways - Google Patents
Biological inducer for removing bottom mud pollutants of black and odorous riverways Download PDFInfo
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- CN112194331A CN112194331A CN202010945715.XA CN202010945715A CN112194331A CN 112194331 A CN112194331 A CN 112194331A CN 202010945715 A CN202010945715 A CN 202010945715A CN 112194331 A CN112194331 A CN 112194331A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention provides a biological inducer for strengthening removal of bottom mud pollutants of a black and odorous river, and particularly relates to the following components: 40-80% of a component A, 10-20% of a component B, 1-5% of a surfactant, 0.5-3% of a stabilizer, 1-3% of a synergist and the balance of water, wherein the component A is sodium acetate, and the component B is one or more of phthalic acid and salicylic acid. The formula has unique principle, simple production and processing and stable property, and is suitable for industrial production and engineering application. The method has the advantages of thorough bottom mud remediation, good effect and low cost, and solves the problems of difficult remediation of the organic matters which pollute the bottom mud and are difficult to degrade and slow biological treatment effect in the prior art.
Description
Technical Field
The invention belongs to the technical field of sediment treatment, and particularly relates to a biological inducer for removing black and odorous river sediment pollutants, namely a combined preparation for artificially inducing indigenous specific flora in the sediment to degrade refractory organic pollutants.
Background
The river channel is used as a main bearing body of an urban water source and has various ecological regulation functions, but with the expanding development of the urbanization process, the urban population is increased, and the water pollution treatment measures and equipment treatment effects are lagged, so that the river channels of many cities directly become main channels and places for industrial, agricultural and domestic sewage discharge. A large amount of high concentration wastewater is discharged into the river and forms siltation in the bottom sludge, forming serious endogenous pollution. At present, the traditional bottom mud remediation method mainly aims at the traditional organic pollution or black and odorous pollution indexes, and aims at the problem that refractory organic matters with serious damage of teratogenesis, carcinogenesis and mutagenesis can only be slowly degraded by using indigenous microorganisms in the bottom mud through a natural metabolic pathway. However, the indigenous microbial populations of the sediment are different, and the specific functional flora capable of degrading the refractory organic matters are also obviously different, so that how to increase the abundance of the functional flora capable of degrading the refractory organic matters in the sediment and promote the fast degradation of the refractory organic pollutants becomes a technical problem in the field of sediment treatment at present.
Sodium acetate, colorless transparent or white granular crystals, relative density: 1.45 (trihydrate), 1.528 (anhydrate), refractive index: 1.464, melting point (. degree. C.) 324. Can be weathered and flammable in the air. Is easily soluble in water, slightly soluble in ethanol and insoluble in diethyl ether; usually the wet process produces an acetic acid taste and hydrolysis occurs in water. Sodium acetate is one of the co-substrates currently known to be widely available to microorganisms.
Phthalic acid, English name is Phthalic acid, CAS number is 88-99-3, molecular formula is C8H6O4Colorless crystals or crystalline powders, poisonSex LD50 (mg/kg): rats were orally administered 7900. The binary aromatic carboxylic acid formed by connecting two carboxyl groups with two adjacent carbon atoms in a benzene ring respectively can replace phthalic acid by phthalic anhydride in application, and is a raw material of synthetic resin, fiber, medicaments and the like.
Salicylic acid of formula C7H6O3Melting point (. degree. C.): 227, density (g/mL, 25/4 ℃ C.): 1.451, is the extract of willow bark, which is a natural anti-inflammatory drug. The common cold medicine aspirin is salicylic acid derivative sodium acetylsalicylate, and para-aminosalicylate (PAS) is a common antitubercular medicine. Slightly soluble in cold water and easily soluble in hot water, ethanol, ether and acetone, and soluble in hot benzene.
The prior art does not report the adoption of the substances or the combination thereof for inducing the black and odorous river sediment to remove pollutants.
Disclosure of Invention
In order to overcome the defects of the existing technology for repairing bottom mud by microorganisms, the invention provides a biological inducer for removing bottom mud pollutants of a black and odorous river, namely a combined preparation for artificially inducing indigenous specific flora in the bottom mud to degrade refractory organic pollutants, so as to promote the abundance of the specific flora capable of degrading the refractory organic pollutants in the bottom mud to be increased. Compared with biological induction without adding a biological inducer or by independently adding the component A, namely sodium acetate, the composite preparation has obvious promotion effect on the increase of the abundance of the high-efficiency degradation flora of the organic matters difficult to degrade in the sediment.
The above object of the present invention is achieved by the following technical solutions:
a biological inducer for removing black and odorous river sediment pollutants specifically comprises the following components: by mass, 40-80% of the component A, 10-20% of the component B, 1-5% of a surfactant, 0.5-3% of a stabilizer, 1-3% of a synergist and the balance of water.
As a preferable technical scheme of the invention, the inducer specifically comprises a component A of sodium acetate, and a component B of phthalic acid and/or salicylic acid.
As a preferred technical scheme of the invention, the surfactant is specifically one or more of rhamnolipid, triton 100 and tween 80.
According to the research of the invention, sodium acetate is used as a primary metabolism matrix of the bottom sediment microorganisms to provide sufficient carbon source and energy for the microorganisms, and the specific microbial flora is induced to metabolize to generate corresponding enzymes to degrade a secondary matrix; the dissolved organic acid in the component B effectively promotes the forward progress of the oxygenizing reaction of the microorganism oxygenase; the macromolecular surfactant effectively promotes the solubilization micelles of the refractory organic matters to be directly utilized by the sediment microbial thalli. Thereby, synergistically inducing and removing the contaminants.
As a preferred technical scheme of the invention, the stabilizer is specifically one or more of disodium ethylene diamine tetraacetate, bentonite, sodium pyrophosphate and sodium nitrate.
As a preferred technical scheme of the invention, the synergist is specifically one or more of methanol, sucrose, glucose, starch and rice straws.
The invention further provides application of the biological inducer for removing black and odorous river sediment pollutants in degradation of organic pollutants in black and odorous river sediment.
Specifically, the inducer effectively promotes the directional degradation of the bottom sediment specific flora on the refractory organic pollutants.
The invention has the beneficial effects that: the invention provides a combined preparation for artificially inducing indigenous specific flora in bottom sediment to degrade refractory organic pollutants, which is used for repairing and controlling the pollution of refractory organic matters in the bottom sediment and has the following advantages:
(1) compared with the existing technology for remedying the bottom sediment by microorganisms, the method can effectively promote the directional degradation of the bottom sediment specific flora on the organic pollutants difficult to degrade, and can effectively improve the remediation rate.
(2) Compared with the prior art of repairing bottom mud by microorganisms, the introduction of the microorganism inducer does not bring additional organic matter pollution.
(3) The invention has a directional induction coefficient of 2, and belongs to obvious induction.
(4) The main component A and the component B related to the combined preparation are organic compounds which are cheap and easy to obtain, do not introduce additional pollution and have extremely high environmental value.
Drawings
FIG. 1 is a schematic diagram of the action process of the combined preparation for artificially inducing indigenous specific flora in the sediment to degrade refractory organic pollutants.
FIG. 2 is a graph showing the results of comparative analyses of population abundance of phyla level microorganisms in sediment microorganism 16S rDNA sequencing after 4 months of culture in examples 1, 2, 3, and 4.
Detailed Description
The present invention will be described in further detail with reference to the following examples and drawings, but the present invention is not limited thereto.
Example 1:
0% sodium acetate, 0% phthalic acid, 3% rhamnolipid, 2% bentonite, 1% starch and the balance water.
Example 2:
10% sodium acetate, 0% phthalic acid, 3% rhamnolipid, 2% bentonite, 1% starch and the balance water.
Example 3:
40% sodium acetate, 0% phthalic acid, 3% rhamnolipid, 2% bentonite, 1% starch and the balance water.
Example 4:
40% sodium acetate, 6% phthalic acid, 3% rhamnolipid, 2% bentonite, 1% starch and the balance water.
Example 5:
(1) design of experiments
The raw medicines are prepared into combined water suspension preparations according to example proportions, namely example 1, example 2, example 3 and example 4.
(2) Test method
Referring to fig. 1, in a serum bottle experiment, the contaminated sediment and the induction preparation are uniformly stirred, placed at the bottom layer of the serum bottle, and pumped by a peristaltic pump into the sediment-water ratio of 1: 1, and culturing in a dark place for 4 months.
(3) Test results and analysis
FIG. 2 is a comparative analysis of the abundance of the phylum level microbial population in sediment microbial 16S rDNA sequencing after 4 months of culture in examples 1, 2, 3, and 4. As can be seen from the figure, the addition of the component A in the combined preparation for artificially inducing the indigenous specificity flora in the sediment to degrade the refractory organic pollutants directionally induces the increase of the abundance of certain flora populations, the increase is more obvious when the proportion is larger, and the addition of the component B further promotes the increase of the abundance of the flora populations. Through analysis, the increased several groups of bacteria are reported high-efficiency degradation bacteria of hardly degradable organic matters in bottom mud or sludge or a water system.
And (4) conclusion: especially, the scheme of the embodiment 4 is most excellent for increasing the abundance of the efficient degrading bacteria.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (7)
1. The biological inducer for removing the bottom mud pollutants of the black and odorous riverway is characterized by comprising the following components: by mass, 40-80% of component A, 10-20% of component B, 1-5% of surfactant, 0.5-3% of stabilizer,
synergist and water in balance.
2. The inducer according to claim 1, wherein the component A is sodium acetate, and the component B is one or two of phthalic acid and salicylic acid.
3. An inducer according to claim 1, characterized in that: the surfactant is one or more of rhamnolipid, triton 100 and tween 80.
4. An inducer according to claim 1, characterized in that: the stabilizer is one or more of disodium ethylene diamine tetraacetate, bentonite, sodium pyrophosphate and sodium nitrate.
5. An inducer according to claim 1, characterized in that: the synergist is one or more of methanol, sucrose, glucose, starch and rice straw.
6. Use of the biological inducer for removing black and odorous river sediment pollutants as defined in any one of claims 1 to 5 in degradation of organic pollutants in black and odorous river sediment.
7. The use according to claim 6, wherein the inducer is effective to promote the targeted degradation of refractory organic pollutants by the sediment-specific flora.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6432693B1 (en) * | 1999-11-15 | 2002-08-13 | Geovation Technologies, Inc. | Advanced inorganic solid-chemical composition and methods for anaerobic bioremediation |
| KR100656288B1 (en) * | 2005-01-17 | 2006-12-11 | 한국해양연구원 | AGENTS FOR ENHANCING BIODEGRADATION RATE OF BENZO[a]PYRENE AND THE METHOD OF BIODEGRADATION WITH SAME |
| US20120252102A1 (en) * | 2011-03-31 | 2012-10-04 | Bioremediation Specialists, LLC | Bioremediation systems, compositions, and methods |
| CN104071957A (en) * | 2014-07-04 | 2014-10-01 | 哈尔滨工业大学深圳研究生院 | Biochemical process for in-situ remediation of black and odorous sediments polluted by organic matters |
| CN104755435A (en) * | 2012-09-10 | 2015-07-01 | 通用电气公司 | Method of reducing residual recalcitrant organic pollutants |
| CN108118022A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | Complete microculture accelerating agent and its application of denitrification process |
| CN109368780A (en) * | 2018-12-27 | 2019-02-22 | 浙江大学常州工业技术研究院 | Saprobia purification and nutrition complexing agent and preparation method thereof |
-
2020
- 2020-09-10 CN CN202010945715.XA patent/CN112194331B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6432693B1 (en) * | 1999-11-15 | 2002-08-13 | Geovation Technologies, Inc. | Advanced inorganic solid-chemical composition and methods for anaerobic bioremediation |
| KR100656288B1 (en) * | 2005-01-17 | 2006-12-11 | 한국해양연구원 | AGENTS FOR ENHANCING BIODEGRADATION RATE OF BENZO[a]PYRENE AND THE METHOD OF BIODEGRADATION WITH SAME |
| US20120252102A1 (en) * | 2011-03-31 | 2012-10-04 | Bioremediation Specialists, LLC | Bioremediation systems, compositions, and methods |
| CN104755435A (en) * | 2012-09-10 | 2015-07-01 | 通用电气公司 | Method of reducing residual recalcitrant organic pollutants |
| CN104071957A (en) * | 2014-07-04 | 2014-10-01 | 哈尔滨工业大学深圳研究生院 | Biochemical process for in-situ remediation of black and odorous sediments polluted by organic matters |
| CN108118022A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | Complete microculture accelerating agent and its application of denitrification process |
| CN109368780A (en) * | 2018-12-27 | 2019-02-22 | 浙江大学常州工业技术研究院 | Saprobia purification and nutrition complexing agent and preparation method thereof |
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