CN112169006A - Composite dressing with antibacterial function and preparation method thereof - Google Patents
Composite dressing with antibacterial function and preparation method thereof Download PDFInfo
- Publication number
- CN112169006A CN112169006A CN202011033183.9A CN202011033183A CN112169006A CN 112169006 A CN112169006 A CN 112169006A CN 202011033183 A CN202011033183 A CN 202011033183A CN 112169006 A CN112169006 A CN 112169006A
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- CN
- China
- Prior art keywords
- solution
- antibacterial
- silk fibroin
- sodium alginate
- composite dressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 107
- 239000002131 composite material Substances 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims description 32
- 108010022355 Fibroins Proteins 0.000 claims abstract description 75
- HPTYUNKZVDYXLP-UHFFFAOYSA-N aluminum;trihydroxy(trihydroxysilyloxy)silane;hydrate Chemical compound O.[Al].[Al].O[Si](O)(O)O[Si](O)(O)O HPTYUNKZVDYXLP-UHFFFAOYSA-N 0.000 claims abstract description 67
- 229910052621 halloysite Inorganic materials 0.000 claims abstract description 67
- 239000002071 nanotube Substances 0.000 claims abstract description 67
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 66
- 239000000661 sodium alginate Substances 0.000 claims abstract description 66
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 66
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 66
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 61
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims abstract description 46
- -1 polyoxyethylene Polymers 0.000 claims abstract description 38
- 239000004148 curcumin Substances 0.000 claims abstract description 23
- 229940109262 curcumin Drugs 0.000 claims abstract description 23
- 235000012754 curcumin Nutrition 0.000 claims abstract description 23
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims abstract description 23
- FTVWIRXFELQLPI-ZDUSSCGKSA-N (S)-naringenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC(O)=C2C(=O)C1 FTVWIRXFELQLPI-ZDUSSCGKSA-N 0.000 claims abstract description 22
- 229940117954 naringenin Drugs 0.000 claims abstract description 22
- WGEYAGZBLYNDFV-UHFFFAOYSA-N naringenin Natural products C1(=O)C2=C(O)C=C(O)C=C2OC(C1)C1=CC=C(CC1)O WGEYAGZBLYNDFV-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000007625 naringenin Nutrition 0.000 claims abstract description 22
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 19
- 239000000243 solution Substances 0.000 claims description 116
- 238000009987 spinning Methods 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 28
- 238000002156 mixing Methods 0.000 claims description 24
- 239000008367 deionised water Substances 0.000 claims description 17
- 229910021641 deionized water Inorganic materials 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000725 suspension Substances 0.000 claims description 15
- 239000002121 nanofiber Substances 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 12
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 12
- 239000012047 saturated solution Substances 0.000 claims description 12
- 238000001291 vacuum drying Methods 0.000 claims description 12
- 239000000835 fiber Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 9
- SLLBSHACCWUQGH-UHFFFAOYSA-M [Li]Br.OC=O Chemical compound [Li]Br.OC=O SLLBSHACCWUQGH-UHFFFAOYSA-M 0.000 claims description 8
- 241000255789 Bombyx mori Species 0.000 claims description 7
- 238000001523 electrospinning Methods 0.000 claims description 7
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 235000019253 formic acid Nutrition 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 230000003115 biocidal effect Effects 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 2
- 208000012886 Vertigo Diseases 0.000 description 34
- 230000000052 comparative effect Effects 0.000 description 19
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- 239000007787 solid Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 231100000135 cytotoxicity Toxicity 0.000 description 4
- 230000003013 cytotoxicity Effects 0.000 description 4
- 230000029663 wound healing Effects 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 230000010355 oscillation Effects 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 206010048038 Wound infection Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000005923 long-lasting effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- XHFLOLLMZOTPSM-UHFFFAOYSA-M sodium;hydrogen carbonate;hydrate Chemical compound [OH-].[Na+].OC(O)=O XHFLOLLMZOTPSM-UHFFFAOYSA-M 0.000 description 2
- AEMOLEFTQBMNLQ-AZLKCVHYSA-N (2r,3s,4s,5s,6r)-3,4,5,6-tetrahydroxyoxane-2-carboxylic acid Chemical compound O[C@@H]1O[C@@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-AZLKCVHYSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-SYJWYVCOSA-N (2s,3s,4s,5s,6r)-3,4,5,6-tetrahydroxyoxane-2-carboxylic acid Chemical compound O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-SYJWYVCOSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910000323 aluminium silicate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 229920001448 anionic polyelectrolyte Polymers 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229920000867 polyelectrolyte Polymers 0.000 description 1
- 229920005594 polymer fiber Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000004154 testing of material Methods 0.000 description 1
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/16—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one other macromolecular compound obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds as constituent
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2400/00—Materials characterised by their function or physical properties
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Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Public Health (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- Toxicology (AREA)
- Inorganic Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Artificial Filaments (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
Abstract
The invention provides a composite dressing with an antibacterial function, which is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with antibacterial components by an electrostatic spinning means; the mass ratio of the sodium alginate to the silk fibroin to the polyoxyethylene is 1: (2-4): (1-3); the halloysite nanotube loaded with the antibacterial component accounts for 4-10% of the mass ratio of the silk fibroin; the antibacterial component is one or a combination of curcumin and naringenin. The composite dressing has excellent biocompatibility and safety, and the mechanical property of the antibacterial dressing is effectively improved by compounding the sodium alginate, the silk fibroin and the polyethylene oxide in a proper proportion and adding a certain amount of halloysite nanotubes loaded with antibacterial components; the halloysite nanotube is adopted to load the antibacterial ingredients, so that the release rate of the antibacterial ingredients is slowed down, and the aim of long-acting antibiosis is fulfilled.
Description
Technical Field
The invention belongs to the technical field of medical dressings, and particularly relates to a composite dressing with an antibacterial function and a preparation method thereof.
Background
The skin is directly contacted with the external environment, and is easily damaged due to injury or diseases. Wound infection is a key factor affecting wound healing, and in order to reduce the probability of wound infection, a dressing is generally used for covering and protecting a wound, providing an environment beneficial to wound healing, and promoting wound healing. Good medical dressings should have excellent antibacterial performance, most medical dressings in the market adopt metal ions such as a certain amount of antibiotics or nano silver with antibacterial performance and the like which are directly added, but the antibiotics can generate drug resistance after long-term use, and the metal ions have potential harm to human bodies; and the release rate of the antibacterial component is high, and the antibacterial effect lasts for a period of time, but generally, in the tissue repair process after major surgery, the infection generally lasts for more than 1 week. Therefore, it is important to develop a dressing that is safe and can slow the release rate of the drug to achieve long-lasting antimicrobial activity.
Sodium alginate (sodium alginate) is a linear anionic natural polysaccharide extracted from seaweed, is polymerized from beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) through (1-4) glycosidic bonds, and has stability, solubility, viscosity and safety required by pharmaceutical preparation auxiliary materials; however, sodium alginate is an anionic polyelectrolyte, and molecular chains of the polyelectrolyte are rigid and extend like worms in an aqueous solution, so that electrostatic spinning of pure sodium alginate is very difficult. Silk fibroin is a natural protein from silkworms and can be processed into a range of materials with controllable mechanical properties and degradation rates. The silk fibroin is mainly composed of nonpolar amino acid, has excellent characteristics of good biocompatibility, small inflammatory reaction, high degradability, good air and moisture permeability, promotion of wound healing and the like, and is widely applied to the field of medical dressings.
The electrostatic spinning technology is a common simple method for preparing nano-fibers, drugs and biological macromolecules are easily loaded into the fibers and on the surfaces of the fibers in the electrostatic spinning or spinning treatment process, and in addition, the antibacterial elements cannot change in performance after being loaded into the fibers, so that the antibacterial performance of the fibers can be maintained, and bacterial infection can be more effectively prevented.
The halloysite nanotube is tubular aluminosilicate, has a loading rate of about 15-20%, a length-diameter ratio of 20-50, can remarkably enhance the mechanical property of the fiber when oriented in polymer fibers, is natural, does not pollute the environment, has good compatibility, and can be widely applied to the aspects of cosmetics, drug slow release and the like when used as a tubular container.
Disclosure of Invention
The invention aims to provide a safe and continuously effective antibacterial composite dressing for overcoming the defects of the existing medical dressing.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the composite dressing with the antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with antibacterial components by an electrostatic spinning method.
Preferably, the mass ratio of the sodium alginate to the silk fibroin to the polyethylene oxide is 1: (2-4): (1-3).
Preferably, the halloysite nanotubes loaded with the antibacterial component account for 4-10% of the mass ratio of the silk fibroin.
Preferably, the antibacterial component is curcumin or naringenin.
Preferably, the antibacterial component is a combination of curcumin and naringenin.
Preferably, the antibacterial component consists of curcumin and naringenin in a mass ratio of 1: 1.
The invention also provides a preparation method of the composite dressing with the antibacterial function, which comprises the following steps:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain sodium alginate solution with mass concentration of 2-3%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into sodium carbonate aqueous solution or sodium bicarbonate aqueous solution or biological enzyme aqueous solution, heating for degumming, dissolving with lithium bromide-formic acid solution, dialyzing, filtering, concentrating, and preparing to obtain silk fibroin solution with mass concentration of 5-8%;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene in deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 2-3%;
s4: preparing the halloysite nanotube loaded with the antibacterial component: adding the antibacterial component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing the halloysite nanotube and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to ensure that the pressure in the halloysite nanotube cavity is negative, repeating the steps twice, centrifuging to remove supernatant, washing the precipitate with deionized water, drying and grinding to obtain the halloysite nanotube loaded with the antibacterial component;
s5: preparing electrostatic mixed spinning solution: preparing a mixed solution from a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution and a halloysite nanotube carrying an antibacterial component, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 20-30kV, the distance from a jet orifice to a receiver to be 10-20cm, and the spinning speed to be 0.5-2mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and carrying out vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
In the step S2, the lithium bromide-formic acid solution is prepared from a lithium bromide solution with a mass concentration of 20-30% and a formic acid solution with a mass concentration of 90-98% in a volume ratio of 1: 1.
The mass ratio of the antibacterial component to the halloysite nanotubes in the step S4 is (1-1.5): 1.
the invention has the beneficial effects that: 1) the composite dressing has excellent biocompatibility, the adopted raw materials of sodium alginate, silk fibroin and halloysite nanotubes are all natural sources, polyoxyethylene is also a nontoxic, biocompatible and biodegradable synthetic polymer, and the main antibacterial components of curcumin and naringenin are plant components extracted from natural Chinese herbal medicines, so that the dressing has better antibacterial property and better safety compared with antibiotics and metal ion antibacterial agents; 2) polyethylene oxide is added in the raw materials, and the polyethylene oxide and sodium alginate have an intermolecular interaction, so that the flexibility and entanglement degree of a molecular chain segment of the sodium alginate are improved, and the electrostatic spinning capacity of the sodium alginate is improved; 3) according to the invention, sodium alginate, silk fibroin and polyethylene oxide are compounded in a proper proportion, and a certain amount of halloysite nanotubes loaded with antibacterial components are added, so that the mechanical property of the composite dressing is effectively improved; 4) the halloysite nanotube is adopted to load the antibacterial components, so that the release rate of the antibacterial components is slowed down, and the aim of long-acting antibiosis of the composite dressing is fulfilled.
Detailed Description
The present invention is further illustrated by the following examples, but the present invention is not limited to these examples.
Example 1
A composite dressing with antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with curcumin by electrostatic spinning;
wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 2: 1; the halloysite nanotube loaded with curcumin accounts for 6% of the mass proportion of the silk fibroin.
Example 2
A composite dressing with antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and a halloysite nanotube carrying naringenin by electrostatic spinning;
wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 2: 1; the halloysite nanotube carrying naringenin accounts for 6% of the mass proportion of the silk fibroin.
Example 3
A composite dressing with antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with antibacterial components by electrostatic spinning;
wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 2: 1; the halloysite nanotube loaded with the antibacterial component accounts for 6% of the mass ratio of the silk fibroin; the antibacterial component is prepared from curcumin and naringenin according to a mass ratio of 1: 1.
The preparation method of the composite antibacterial dressing of examples 1-3 is as follows:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain a sodium alginate solution with the mass concentration of 2%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into sodium carbonate aqueous solution, heating for degumming, dissolving with lithium bromide-formic acid solution (composed of 20 wt% lithium bromide solution and 98 wt% formic acid solution at volume ratio of 1: 1), dialyzing, filtering, concentrating, and preparing into 5% silk fibroin solution;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene into deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 2%;
s4: preparing the halloysite nanotube loaded with the antibacterial component: adding 3g of antibacterial component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing 3g of halloysite nanotube and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to make the inner cavity of the halloysite nanotube have negative pressure, repeating the steps twice, centrifuging to remove supernatant, washing the precipitate with deionized water, drying and grinding to obtain the halloysite nanotube loaded with the antibacterial component;
s5: preparing electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 2: 1, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 6% of the mass of silk fibroin and carries an antibacterial component, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: and (2) injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 25kV, adjusting the distance from a jet orifice to a receiver to be 15cm, adjusting the spinning speed to be 1mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and performing vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
Example 4
A composite dressing with antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with antibacterial components by electrostatic spinning;
wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 3: 2; the halloysite nanotube loaded with the antibacterial component accounts for 8% of the mass ratio of the silk fibroin; the antibacterial component is prepared from curcumin and naringenin according to a mass ratio of 1: 1.
The preparation method of the composite antibacterial dressing comprises the following steps:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain a sodium alginate solution with the mass concentration of 2%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into sodium bicarbonate water solution, heating for degumming, dissolving with lithium bromide-formic acid solution (composed of 30 wt% lithium bromide solution and 95 wt% formic acid solution at volume ratio of 1: 1), dialyzing, filtering, concentrating, and preparing to obtain 6% silk fibroin solution;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene into deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 3%;
s4: preparing the halloysite nanotube loaded with the antibacterial component: adding 3.5g of antibacterial component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing 2.5g of halloysite nanotube and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to ensure that the pressure in the tube cavity of the halloysite nanotube is negative, repeating the steps twice, centrifuging to remove supernatant, drying and grinding the precipitate to obtain the halloysite nanotube loaded with the antibacterial component;
s5: preparing electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 3: 2, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 8% of the mass of silk fibroin and carries an antibacterial component, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: and (2) injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 20kV, adjusting the distance from a jet orifice to a receiver to be 15cm, adjusting the spinning speed to be 1.5mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and performing vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
Example 5
A composite dressing with antibacterial function is prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with curcumin by electrostatic spinning;
wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 4: 2; the halloysite nanotube loaded with curcumin accounts for 4% of the mass proportion of the silk fibroin.
The preparation method of the composite antibacterial dressing comprises the following steps:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain a sodium alginate solution with the mass concentration of 3%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into a biological enzyme aqueous solution, heating for degumming, dissolving by a lithium bromide-formic acid solution (comprising a lithium bromide solution with the mass concentration of 20 wt% and a formic acid solution with the mass concentration of 90 wt% according to the volume ratio of 1: 1), dialyzing, filtering, concentrating, and preparing to obtain a silk fibroin solution with the mass concentration of 8%;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene into deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 3%;
s4: preparation of the halloysite nanotubes loaded with curcumin: adding 3.6g of curcumin component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing 2.4g of halloysite nanotubes and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to ensure that the pressure in the tube cavity of the halloysite nanotubes is negative, repeating the steps twice, centrifuging to remove supernatant, washing precipitates with deionized water, drying and grinding to obtain the halloysite nanotubes loaded with curcumin;
s5: preparing electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 4: 2, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 4% of the mass of the silk fibroin and is loaded with curcumin, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: and (2) injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 30kV, adjusting the distance from a jet orifice to a receiver to be 20cm, and the spinning speed to be 0.5mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and carrying out vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
Example 6
The composite dressing with the antibacterial function is characterized by being prepared from sodium alginate, silk fibroin, polyethylene oxide and a halloysite nanotube carrying naringenin by an electrostatic spinning method.
Wherein the mass ratio of sodium alginate to silk fibroin to polyoxyethylene is 1: 2: 3; the halloysite nanotube carrying naringenin accounts for 10% of the mass proportion of the silk fibroin.
The preparation method of the composite antibacterial dressing comprises the following steps:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain a sodium alginate solution with the mass concentration of 2%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into sodium bicarbonate water solution, heating for degumming, dissolving with lithium bromide-formic acid solution (composed of 25 wt% lithium bromide solution and 95 wt% formic acid solution at volume ratio of 1: 1), dialyzing, filtering, concentrating, and preparing to obtain 7% silk fibroin solution;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene into deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 2%;
s4: preparation of the halloysite nanotube loaded with naringenin: adding 3.3g of naringenin component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing 2.7g of halloysite nanotube and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to ensure that the pressure in the tube cavity of the halloysite nanotube is negative, repeating the steps twice, centrifuging to remove the supernatant, washing the precipitate with deionized water, drying and grinding to obtain the halloysite nanotube carrying the naringenin;
s5: preparing electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 2: 3, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 10% of the mass of silk fibroin and is loaded with naringenin, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: and (2) injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 30kV, adjusting the distance from a jet orifice to a receiver to be 10cm, adjusting the spinning speed to be 2mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and performing vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
Comparative example 1
A composite dressing in which the halloysite nanotubes loaded with curcumin were directly replaced with nano-silver loaded halloysite nanotubes, compared to the composite dressing of example 1. Other components, contents and preparation method are unchanged.
Comparative example 2
Compared with the composite dressing in the embodiment 4, the halloysite nanotubes loaded with the antibacterial component are directly replaced by the antibacterial component consisting of curcumin and naringenin according to the mass ratio of 1:1, and other components are unchanged.
Step S4 is omitted from the preparation method, and step S5 is preparation of an electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 2: 1, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding an antibacterial component accounting for 6% of the mass of the silk fibroin, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution.
Comparative example 3
Compared with the composite dressing in example 4, the composite dressing has the mass ratio of sodium alginate to silk fibroin to polyethylene oxide of 1: 1: 4, the antibacterial component is unchanged.
The preparation method comprises the following steps of S5 preparation of electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 1: 4, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 8% of the mass of silk fibroin and is loaded with an antibacterial component, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution; the other steps are the same.
Comparative example 4
Compared with the composite dressing in example 5, the halloysite nanotubes loaded with curcumin account for 3% of the mass proportion of silk fibroin, and other components and contents are unchanged.
The preparation method comprises the following steps of S5 preparation of electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 4: 2, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 3% of the mass of the silk fibroin and is loaded with curcumin, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution; the other steps are the same.
Comparative example 5
Compared with the composite dressing in example 6, the halloysite nanotubes loaded with naringenin account for 12% of the mass proportion of silk fibroin, and other components and contents are unchanged.
The preparation method comprises the following steps of S5 preparation of electrostatic mixed spinning solution: according to the solid mass ratio of sodium alginate to silk fibroin to polyoxyethylene of 1: 2: 3, mixing a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution, adding a halloysite nanotube which is 12% of the mass of silk fibroin and is loaded with naringenin, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution; the other steps are the same.
To illustrate the effect of the composite dressing with antibacterial function of the present invention, the following method was used for testing.
First, cytotoxicity test
The composite dressings prepared in examples 1 to 6 and comparative example 1 were subjected to cytotoxicity evaluation test (test according to national standard GB/T16886.5-2003) to compare examples 1 to 6 and comparative example 1.
The cytotoxicity detection results show that after the cells of the examples 1-6 are co-cultured with human fibroblasts for 24 hours and 48 hours, the corresponding relative cell proliferation rate is more than 92%, and the cytotoxicity rating is 0 grade, so that the cells have good cell compatibility. In contrast, comparative example 1, which was co-cultured with human fibroblasts for 24 hours and 48 hours, had a relative cell proliferation rate of about 80% and a cytotoxicity rating of 1, and was slightly cytotoxic. The composite dressing with the antibacterial function has better safety.
Second, tensile mechanical property test
The composite dressings prepared in examples 1, 4, 5 and 6 of the present invention and comparative examples 2, 3, 4 and 5 were cut into test strips of the same size, both ends of the test strips were wrapped with tinfoil paper, and the tensile mechanical properties were measured while leaving the middle 20mm long. And (3) performing mechanical property test on an Instron5967 universal material testing machine, wherein the stretching speed is 5mm/min, and calculating the breaking strength and the breaking elongation by taking the average value after 5 times of measurement. The results are shown in table 1:
table 1 analysis of tensile properties of different dressings
| Sample (I) | Maximum breaking strength (Mpa) | Elongation at Break (%) |
| Example 1 | 1.56±0.16 | 53.07±8.24 |
| Example 4 | 1.85±0.21 | 55.80±10.03 |
| Example 5 | 1.62±0.25 | 48.65±7.81 |
| Example 6 | 1.74±0.13 | 51.50±9.16 |
| Comparative example 2 | 0.55±0.17 | 28.95±6.54 |
| Comparative example 3 | 1.28±0.19 | 36.54±7.30 |
| Comparative example 4 | 1.07±0.10 | 33.78±5.92 |
| Comparative example 5 | 1.93±0.34 | 27.06±6.07 |
As can be seen from the comparison of the mechanical property data in the table, the mechanical property of the composite dressing product of the present invention is significantly improved, and the mechanical property of example 4 is the best. In addition, compared with the comparative example 3 and the example, the maximum breaking strength and the elongation at break of the composite material are lower than the data of the example, which shows that when the mass ratio of the sodium alginate to the silk fibroin to the polyethylene oxide is 1: (2-4): and (1-3), the composite dressing has better mechanical property.
Compared with example 6, the maximum breaking strength of comparative example 5 is higher than that of example 6, but the breaking elongation is obviously lower than that of example 6, the mechanical property data of comparative example 2 is obviously lower than that of example 4, and the mechanical property data of comparative example 4 is obviously lower than that of example 5, which shows that the addition of the halloysite nanotube loaded with the antibacterial component can effectively improve the mechanical property of the electrospinning material, but when the content is not within 4-10% of the mass ratio of silk fibroin, the mechanical property is obviously influenced.
Third, antibacterial effect test
The composite dressings of examples 1 to 6 and comparative example 2 were each sampled, cut into a size of 1.0cm × 1.0cm, placed in a 250ml Erlenmeyer flask, 70ml of PBS and 5ml of bacterial suspension were added, respectively, and the concentration of bacterial suspension in PBS was adjusted to 1 × 104-9×104CFU/ml, the Erlenmeyer flask is fixed on a shaking table, after shaking at 150r/min for 24h, the sample solution of the experimental group (added sample) and the blank group (not added sample) are respectively taken, after being diluted properly by PBS, the plate is inoculated by agar pouring method, and colony counting is carried out. At the same time, a control group should be established, and only PBS and bacterial suspension are added into the control group. The strain is selected from Escherichia coli E.C and Staphylococcus aureus S.a. The bacteriostasis rate is the ratio (in percentage) of the difference of the average colony number of the tested sample before and after oscillation to the average colony number of the sample before oscillation.
The number of colonies of the sample-free group is 1 multiplied by 104-9×104And the difference between the CFU/ml and the average colony number of the group before and after oscillation is within 10 percent, and the difference between the experimental group bacteriostatic rate and the blank group bacteriostatic rate is more than 26 percent, namely the product has the antibacterial effect. The results are shown in tables 2 and 3.
Table 2 antimicrobial testing of composite dressing pairs E.C and S.a
TABLE 3 antibacterial Effect of composite dressings on E.C and S.a
As can be seen from tables 2 and 3, the composite dressing of the present invention has excellent antibacterial effects against both escherichia coli and staphylococcus aureus, and the antibacterial effect gradually decreases with the lapse of time, but still has antibacterial effect on day 7 and antibacterial effect against escherichia coli on day 11. The composite dressing with the antibacterial function has a long-acting antibacterial effect. In addition, the composite dressings of examples 3 and 4 still have antibacterial effects on both bacteria at day 10, which shows that the two components of curcumin and naringenin are loaded through the halloysite nanotube in a compounding manner, so that a synergistic effect is generated, and the antibacterial effect of the composite dressings is enhanced.
The composite dressing of comparative example 2 had no antibacterial effect on escherichia coli on day 7, and had no antibacterial effect on staphylococcus aureus on day 5, and had no long-lasting bacteriostatic effect as compared with examples 1-6. The invention is proved that the release rate of the antibacterial component is slowed down by loading the antibacterial component by using the halloysite nanotube, thereby realizing the aim of long-acting antibiosis.
It should be noted that the specific embodiments are only representative examples of the present invention, and obviously, the technical solutions of the present invention are not limited to the above examples, and technical solutions that are undoubtedly obtained by those skilled in the art according to the disclosure of the present invention all belong to the protection scope of the present invention.
Claims (9)
1. The composite dressing with the antibacterial function is characterized by being prepared from sodium alginate, silk fibroin, polyethylene oxide and halloysite nanotubes loaded with antibacterial components by an electrostatic spinning method.
2. The composite dressing with antibacterial function according to claim 1, wherein the mass ratio of sodium alginate to silk fibroin to polyethylene oxide is 1: (2-4): (1-3).
3. The composite dressing with the antibacterial function according to claim 1, wherein the halloysite nanotubes loaded with the antibacterial component account for 4-10% of the mass proportion of the silk fibroin.
4. The composite dressing with antibacterial function according to claim 1, wherein the antibacterial component is curcumin or naringenin.
5. The composite dressing with antibacterial function according to claim 1, characterized in that the antibacterial component is a combination of curcumin and naringenin.
6. The composite dressing with antibacterial function according to claim 5, wherein the antibacterial component consists of curcumin and naringenin in a mass ratio of 1: 1.
7. The method for preparing a composite dressing having an antibacterial function according to any one of claims 1 to 6, comprising the steps of:
s1: preparation of sodium alginate solution: dissolving sodium alginate in deionized water, and stirring at normal temperature to obtain sodium alginate solution with mass concentration of 2-3%;
s2: preparing a silk fibroin solution: placing natural silkworm shell or silk into sodium carbonate aqueous solution or sodium bicarbonate aqueous solution or biological enzyme aqueous solution, heating for degumming, dissolving with lithium bromide-formic acid solution, dialyzing, filtering, concentrating, and preparing to obtain silk fibroin solution with mass concentration of 5-8%;
s3: preparation of polyethylene oxide solution: dissolving polyoxyethylene in deionized water, and stirring at normal temperature to prepare a polyoxyethylene solution with the mass concentration of 2-3%;
s4: preparing the halloysite nanotube loaded with the antibacterial component: adding the antibacterial component into ethanol, fully stirring and ultrasonically dissolving to obtain a saturated solution A, fully and uniformly mixing the halloysite nanotube and the saturated solution A to obtain a suspension B, vacuumizing the suspension B for 30min to ensure that the pressure in the halloysite nanotube cavity is negative, repeating the steps twice, centrifuging to remove supernatant, washing the precipitate with deionized water, drying and grinding to obtain the halloysite nanotube loaded with the antibacterial component;
s5: preparing electrostatic mixed spinning solution: preparing a mixed solution from a sodium alginate solution and a silk fibroin solution, adding a polyoxyethylene solution and a halloysite nanotube carrying an antibacterial component, magnetically stirring and mixing, and uniformly oscillating by adopting ultrasonic waves to obtain an electrostatic mixed spinning solution;
s6: preparing a nanofiber membrane: injecting the electrostatic mixed spinning solution into an injector of an electrostatic spinning device, adjusting the electrospinning voltage to be 20-30kV, the distance from a jet orifice to a receiver to be 10-20cm, and the spinning speed to be 0.5-2mL/h, starting the spinning device, collecting the electrospun nanofibers on the receiver, and carrying out vacuum drying on the collected fiber membrane in a vacuum drying oven for 2 days to obtain the composite dressing with the antibacterial function.
8. The method for preparing a composite dressing having an antibacterial function according to claim 7, wherein the lithium bromide-formic acid solution in step S2 is prepared from a lithium bromide solution with a mass concentration of 20-30% and a formic acid solution with a mass concentration of 90-98% in a volume ratio of 1: 1.
9. The method for preparing the composite dressing with the antibacterial function according to claim 7, wherein the mass ratio of the antibacterial component to the halloysite nanotubes in the step S4 is (1-1.5): 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112979999A (en) * | 2021-02-25 | 2021-06-18 | 南通纺织丝绸产业技术研究院 | Biological macromolecule and modified halloysite composite hydrogel and preparation and application thereof |
| CN113604958A (en) * | 2021-08-11 | 2021-11-05 | 浙江理工大学 | Preparation method and application of anti-infection composite layer medical dressing |
| CN114984305A (en) * | 2022-07-19 | 2022-09-02 | 中国人民解放军总医院第一医学中心 | Novel antibacterial film-forming liquid medical dressing and preparation method thereof |
| CN115896979A (en) * | 2022-12-30 | 2023-04-04 | 东北农业大学 | Naringenin cyclodextrin inclusion compound nanofiber and preparation method and application thereof |
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2020
- 2020-09-27 CN CN202011033183.9A patent/CN112169006A/en active Pending
Cited By (6)
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| CN112979999A (en) * | 2021-02-25 | 2021-06-18 | 南通纺织丝绸产业技术研究院 | Biological macromolecule and modified halloysite composite hydrogel and preparation and application thereof |
| CN112979999B (en) * | 2021-02-25 | 2022-11-11 | 南通纺织丝绸产业技术研究院 | Biological macromolecule and modified halloysite composite hydrogel and preparation and application thereof |
| CN113604958A (en) * | 2021-08-11 | 2021-11-05 | 浙江理工大学 | Preparation method and application of anti-infection composite layer medical dressing |
| CN114984305A (en) * | 2022-07-19 | 2022-09-02 | 中国人民解放军总医院第一医学中心 | Novel antibacterial film-forming liquid medical dressing and preparation method thereof |
| CN115896979A (en) * | 2022-12-30 | 2023-04-04 | 东北农业大学 | Naringenin cyclodextrin inclusion compound nanofiber and preparation method and application thereof |
| CN115896979B (en) * | 2022-12-30 | 2023-09-29 | 东北农业大学 | Naringenin cyclodextrin inclusion compound nanofiber as well as preparation method and application thereof |
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Application publication date: 20210105 |