CN112162074A - Multi-environmental factor joint exposure device suitable for sea cucumbers - Google Patents

Multi-environmental factor joint exposure device suitable for sea cucumbers Download PDF

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Publication number
CN112162074A
CN112162074A CN202011069877.8A CN202011069877A CN112162074A CN 112162074 A CN112162074 A CN 112162074A CN 202011069877 A CN202011069877 A CN 202011069877A CN 112162074 A CN112162074 A CN 112162074A
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CN
China
Prior art keywords
water
exposure
tank
feeding
making
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Pending
Application number
CN202011069877.8A
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Chinese (zh)
Inventor
廖国祥
李西山
张玉露
鞠忠磊
厉男
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National Marine Environmental Monitoring Center
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National Marine Environmental Monitoring Center
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Priority to CN202011069877.8A priority Critical patent/CN112162074A/en
Publication of CN112162074A publication Critical patent/CN112162074A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18Water
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/30Culture of aquatic animals of sponges, sea urchins or sea cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/80Feeding devices
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • A01K63/065Heating or cooling devices
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a multi-environmental factor combined exposure device suitable for sea cucumbers, which is characterized in that: the device comprises a wave generating water tank, wherein a wave generating mechanism and a lifting driving mechanism are respectively arranged at two ends of the wave generating water tank, and a heater is also arranged in the wave-making water tank, the lifting driving mechanism can drive the sampling pipe with the electromagnetic valve to move longitudinally, the top end of the sampling pipe is connected with the inlet end of the water-oil concentration on-line detector through a pipeline with a peristaltic pump, the outlet end of the water-oil concentration on-line detector is connected with a plurality of water distribution tanks in parallel through a pipeline with an electromagnetic valve, the combined exposure device also comprises a water bath exposure box, a plurality of sea cucumber exposure boxes are arranged in the water bath exposure box, each water distribution box is communicated with one sea cucumber exposure box through a pipeline with an electromagnetic valve, meanwhile, a heater and a circulating pump are further arranged in the water bath exposure box, and an automatic bait casting mechanism is further arranged above the water bath exposure box.

Description

Multi-environmental factor joint exposure device suitable for sea cucumbers
Technical Field
The invention relates to the field of holothurian culture and environmental impact evaluation, in particular to a multi-environmental factor combined exposure device suitable for holothurian.
Background
In recent years, oil spill accidents in offshore areas of China frequently occur, so that a large number of petroleum hydrocarbon compounds enter ocean water, and the health of the marine ecological environment and the survival of marine organisms in offshore areas of China are seriously threatened. At the same time, human social and industrial activities make CO2The emission of greenhouse gases increases sharply, causing the global warming to be gradually increased, and further causing the temperature of offshore seawater to rise abnormally and the dissolved oxygen in the ocean to drop remarkably. Researches show that the behavior of oil spill in seawater and the change of solubility components can be influenced by the changes of temperature, illumination and seawater disturbance, the changes of the temperature, the illumination and the seawater disturbance can also directly influence the physiological behavior of sea cucumbers, sea cucumber culture areas are mainly distributed in the offshore areas of the yellow and Bohai sea and are very easily influenced by the changes of environmental factors, the changes of multi-environmental factors can greatly influence the sea cucumber culture industry, but the research on the influence mechanism of marine organisms caused by the combined stress generated by the changes of the multi-environmental factors is rare at present, and the experimental device for simulating the combined exposure of the multi-environmental factors such as oil spill pollution, high temperature, dissolved oxygen change, illumination and seawater disturbance is mainly attributed to the absence of the experimental device at present.
Due to the special properties of easy volatilization, easy degradation, difficult dissolution and the like of petroleum hydrocarbon compounds, the behavior of the ocean spilled oil on different seawater space layers is classified into flooding and the solubility components have different changes, so that the influence of the spilled oil on the physiological effect of the sea cucumbers on different seawater space layers under the real condition can be effectively simulated to form a great problem, and in the three main modes of water body exposure, the still water exposure type does not supplement exposure liquid, and the long-term exposure experiment cannot be carried out; the semi-still water exposure type concentration can fluctuate repeatedly within a certain time, and the experimental result is influenced; the accuracy of the experimental result can be ensured only by the flowing water exposure mode; the biological rhythm of the sea cucumber needs to be kept stable during the exposure experiment, and an exposure device which can meet the requirements of automatic feeding and timed illumination in a laboratory is urgently needed.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides an exposure device which can maintain the biological rhythm of the sea cucumber, can simulate the influence of combined exposure of multiple environmental factors such as oil overflow pollution, high temperature, dissolved oxygen change, seawater disturbance and the like on the growth of the sea cucumber on the spatial level of different sea water by using a running water exposure type exposure method.
The technical solution of the invention is as follows: the utility model provides a multi-environment factor joint exposure device suitable for sea cucumber which characterized in that: the device comprises a wave generating water tank 1, wherein a wave generating mechanism and a lifting driving mechanism are respectively arranged at two ends of the wave generating water tank 1, a heater 2 is also arranged in the wave generating water tank 1, the lifting driving mechanism can drive a sampling pipe 4 with an electromagnetic valve 3 to move longitudinally, the top end of the sampling pipe 4 is connected with the inlet end of an online water-oil concentration detector 6 through a pipeline with a peristaltic pump 5, the outlet end of the online water-oil concentration detector 6 is connected with a plurality of water distribution tanks 7 in parallel through pipelines with the electromagnetic valve 3, the combined exposure device further comprises a water bath exposure tank 8, a plurality of sea cucumber exposure tanks 9 are arranged in the water bath exposure tank 8, each water distribution tank 7 is communicated with one sea cucumber exposure tank 9 through a pipeline with the electromagnetic valve 3, and a heater 2 and a circulating pump 10 are also arranged in the water bath exposure tank 8, the water bath exposes the top of case 8 and still is provided with automatic mechanism of feeding, expose the device and still include nitrogen cylinder 11 and oxygen cylinder 12, nitrogen cylinder 11 and oxygen cylinder 12 are parallelly connected with all water distribution tank 7 respectively through the pipeline that has gas flow control valve.
The wave making mechanism comprises a wave making motor 13 fixedly supported on a wave making water tank 1, the output end of the wave making motor 13 is provided with a cam 14, the cam 14 is hinged with one end of a wave making connecting rod 15, the other end of the wave making connecting rod 15 is rotatably connected onto a wave making plate 16, and the wave making plate 16 is slidably connected onto a sliding rail 17 arranged on the wave making water tank 1.
The lifting driving mechanism comprises a lifting motor 18 fixedly supported on the wave-making water tank 1, the output end of the lifting motor 18 is connected with a gear which is rotatably supported in the support 19 through a belt pulley transmission pair, the gear is meshed with a rack 20 which is slidably connected in the support 19, a supporting platform 21 is connected onto the rack 20, the sampling tube 4 is fixedly supported on the supporting platform 21, and the support 19 is arranged in the wave-making water tank 1.
Automatic mechanism of feeding includes support frame 22, support frame 22's both ends are rotated respectively and are supported there are drive roll 23 and driven roll 24, support jointly on drive roll 23 and the driven roll 24 has conveyer belt 25, are provided with the platform of feeding 26 on the conveyer belt 25, are provided with automatic ware of feeding 27 on the platform of feeding 26, still be provided with the motor 28 of feeding on support frame 22, the output of the motor 28 of feeding passes through belt pulley drive pair and links to each other with drive roll 23, still is provided with the light that has the timer in support frame 22's bottom.
A pH probe 29 and a dissolved oxygen probe 30 are arranged in the water distribution tank 7.
Compared with the prior art, the invention has the following advantages:
the multi-environmental factor combined exposure device applicable to the sea cucumbers in the structural form is simple in structure, ingenious in design and reasonable in layout. Aiming at the current situation of the traditional sea cucumber exposure research device, a special structure is designed. The method can maintain the biological rhythm of the sea cucumber, and can simulate multiple environmental factors such as oil overflow pollution, high temperature, dissolved oxygen change, seawater disturbance and the like on different seawater space layers by utilizing a running water exposure type exposure method, so that scientific research is carried out on what influence the multi-parameter combined exposure can generate on the growth of the sea cucumber. The exposure device has the advantages of simple manufacturing process and low manufacturing cost, so the exposure device has various advantages, is particularly suitable for popularization and application in the field, and has very wide market prospect.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Detailed Description
The following description will explain embodiments of the present invention with reference to the accompanying drawings. As shown in fig. 1: a multi-environmental factor combined exposure device suitable for sea cucumbers comprises a wave generating water tank 1, wherein a wave generating mechanism and a lifting driving mechanism are respectively arranged at two ends of the wave generating water tank 1, a heater 2 is further arranged in the wave generating water tank 1, the lifting driving mechanism can drive a sampling pipe 4 with an electromagnetic valve 3 to move longitudinally, the top end of the sampling pipe 4 is connected with the inlet end of an online water-oil concentration detector 6 through a pipeline with a peristaltic pump 5, the outlet end of the online water-oil concentration detector 6 is connected with a plurality of water distribution tanks 7 in parallel through pipelines with the electromagnetic valve 3, the combined exposure device further comprises a water bath exposure tank 8, a plurality of sea cucumber exposure tanks 9 are arranged in the water bath exposure tank 8, each water distribution tank 7 is communicated with one sea cucumber exposure tank 9 through a pipeline with the electromagnetic valve 3, and a heater 2 and a circulating pump 10 are further arranged in the water bath exposure tank 8, the water bath exposes the top of case 8 and still is provided with automatic mechanism of feeding, expose the device and still include nitrogen cylinder 11 and oxygen cylinder 12, nitrogen cylinder 11 and oxygen cylinder 12 are parallelly connected with all water distribution tank 7 respectively through the pipeline that has gas flow control valve.
The wave making mechanism comprises a wave making motor 13 fixedly supported on a wave making water tank 1, the output end of the wave making motor 13 is provided with a cam 14, the cam 14 is hinged with one end of a wave making connecting rod 15, the other end of the wave making connecting rod 15 is rotatably connected onto a wave making plate 16, and the wave making plate 16 is slidably connected onto a sliding rail 17 arranged on the wave making water tank 1.
The lifting driving mechanism comprises a lifting motor 18 fixedly supported on the wave-making water tank 1, the output end of the lifting motor 18 is connected with a gear which is rotatably supported in the support 19 through a belt pulley transmission pair, the gear is meshed with a rack 20 which is slidably connected in the support 19, a supporting platform 21 is connected onto the rack 20, the sampling tube 4 is fixedly supported on the supporting platform 21, and the support 19 is arranged in the wave-making water tank 1.
Automatic mechanism of feeding includes support frame 22, support frame 22's both ends are rotated respectively and are supported there are drive roll 23 and driven roll 24, support jointly on drive roll 23 and the driven roll 24 has conveyer belt 25, are provided with the platform of feeding 26 on the conveyer belt 25, are provided with automatic ware of feeding 27 on the platform of feeding 26, still be provided with the motor 28 of feeding on support frame 22, the output of the motor 28 of feeding passes through belt pulley drive pair and links to each other with drive roll 23, still is provided with the light that has the timer in support frame 22's bottom.
A pH probe 29 and a dissolved oxygen probe 30 are arranged in the water distribution tank 7.
The working process of the multi-environment factor combined exposure device suitable for the sea cucumbers in the embodiment of the invention is as follows: firstly, sea water and exposed oil are added into a wave-making water tank 1, then an instruction is sent out through a control system of the device to control the wave-making mechanism to work, waves with certain frequency are created in the wave-making water tank 1, meanwhile, a heater 2 can also heat water in the wave-making water tank 1, in the process, a sampling pipe 4 of a lifting driving mechanism moves to enable the wave-making mechanism to hover at different water depths, power is provided by a peristaltic pump 5, collected water samples are input into a water-oil concentration online detector 6 to detect the concentrations of the oil in the water at different depths, the water samples at different depths are input into different water distribution tanks 7 through the control of electromagnetic valves 3 on the different water distribution tanks 7 after detection, and the aeration rate of the nitrogen and the oxygen in each water distribution tank 7 is adjusted according to data detected by a pH probe 29 and a dissolved oxygen probe 30 in each water distribution tank 7 (by adjusting a gas flow control valve on a nitrogen bottle 11 and an oxygen bottle 12) ,
after the aeration operation is finished, opening the electromagnetic valve 3 connected on the pipeline between the water distribution tank 7 and the sea cucumber exposure tank 9, inputting the water samples subjected to aeration treatment into the sea cucumber exposure tank 9, wherein each sea cucumber exposure tank 9 is cultured with the tested sea cucumber, and the oil concentration condition of the polluted seawater in different water depths is judged by observing the specific culture condition of the tested sea cucumber in different water sample environments; in the culture process, the heater 2 in the water bath exposure box 8 is started, and the circulating pump 10 is started at the same time, so that the temperature of the water in the water bath exposure box 8 can be quickly and uniformly adjusted, and the aim of indirectly adjusting the water temperature in all the sea cucumber exposure boxes 9 is fulfilled;
in the process of culturing the sea cucumbers, the automatic feeding mechanism positioned above the plurality of sea cucumber exposing boxes 9 works to feed the sea cucumbers cultured in each sea cucumber exposing box 9;
the working process of the wave generating mechanism is as follows: the control system controls the wave making motor 13 to work, the wave making motor 13 drives the cam 14 to rotate, the wave making connecting rod 15 is driven to swing, the wave making connecting rod 15 finally drives the wave making plate 16 to reciprocate along the slide rail 17, and finally waves with certain frequency are simulated in the wave making water tank 1;
the working process of the lifting driving mechanism is as follows: the control system controls the lifting motor 18 to work, the lifting motor 18 drives the gear pair to rotate through the belt pulley, and then the rack 20 meshed with the gear is driven to do longitudinal linear motion in the support 19, and finally the horizontal height of the sampling pipe 4 fixedly supported on the supporting platform 21 is changed, so that the purpose that the sampling pipe 4 performs sampling at different water depths is achieved;
the automatic bait casting mechanism has the working process as follows: the control system controls the bait casting motor 28 to work, the bait casting motor 28 drives the driving roller 23 to rotate through the belt pulley transmission pair, the conveying belt 25 is driven to move, the bait casting platform 26 can be controlled to stop above a certain sea cucumber exposure box 9 in a mode of controlling the revolution number of the bait casting motor 28, after the bait casting motor 28 stops, the control system obtains signals and controls the automatic bait casting device 27 to work, bait placed in the automatic bait casting device 27 in advance is cast in the corresponding sea cucumber exposure box 9, and the steps are repeated until all the automatic bait casting devices 27 complete bait casting operation in all the sea cucumber exposure boxes 9 in sequence.

Claims (5)

1. The utility model provides a multi-environment factor joint exposure device suitable for sea cucumber which characterized in that: the device comprises a wave generating water tank (1), wherein a wave generating mechanism and a lifting driving mechanism are respectively arranged at two ends of the wave generating water tank (1), a heater (2) is further arranged in the wave generating water tank (1), the lifting driving mechanism can drive a sampling pipe (4) with an electromagnetic valve (3) to longitudinally move, the top end of the sampling pipe (4) is connected with the inlet end of an online water-oil concentration detector (6) through a pipeline with a peristaltic pump (5), the outlet end of the online water-oil concentration detector (6) is connected with a plurality of water distribution tanks (7) in parallel through a pipeline with the electromagnetic valve (3), the combined exposure device further comprises a water bath exposure tank (8), a plurality of sea cucumber exposure tanks (9) are arranged in the water bath exposure tank (8), and each water distribution tank (7) is communicated with one sea cucumber exposure tank (9) through a pipeline with the electromagnetic valve (3), still be provided with heater (2) and circulating pump (10) in water bath exposure case (8) simultaneously, the water bath exposes the top of case (8) and still is provided with automatic mechanism of feeding, it still includes nitrogen cylinder (11) and oxygen cylinder (12) to expose the device, nitrogen cylinder (11) and oxygen cylinder (12) are parallelly connected with all water distribution tank (7) respectively through the pipeline that has gas flow control valve.
2. The multi-environmental-factor joint exposure apparatus for sea cucumbers according to claim 1, wherein: make ripples mechanism and include that a fixed stay makes ripples motor (13) on making ripples water tank (1), the output of making ripples motor (13) is provided with cam (14), and cam (14) are articulated mutually with the one end of making ripples connecting rod (15), and the other end of making ripples connecting rod (15) then rotates to be connected on making ripples board (16), make ripples board (16) then sliding connection on setting up slide rail (17) on making ripples water tank (1).
3. The multi-environmental-factor joint exposure apparatus for sea cucumbers according to claim 1, wherein: the lifting driving mechanism comprises a lifting motor (18) fixedly supported on the wave-making water tank (1), the output end of the lifting motor (18) is connected with a gear which is rotatably supported in the support (19) through a belt pulley transmission pair, the gear is meshed with a rack (20) which is slidably connected in the support (19), a supporting platform (21) is connected onto the rack (20), the sampling tube (4) is fixedly supported on the supporting platform (21), and the support (19) is arranged in the wave-making water tank (1).
4. The multi-environmental-factor joint exposure apparatus for sea cucumbers according to claim 1, wherein: automatic mechanism of feeding includes support frame (22), the both ends of support frame (22) are rotated respectively and are supported drive roll (23) and driven roll (24), it has conveyer belt (25) to support jointly on drive roll (23) and driven roll (24), is provided with on conveyer belt (25) platform (26) of feeding, is provided with automatic ware (27) of feeding on platform (26) of feeding, still be provided with on support frame (22) motor (28) of feeding, the output of motor (28) of feeding passes through belt pulley transmission pair and links to each other with drive roll (23), still is provided with the light that has the timer in the bottom of support frame (22).
5. The multi-environmental-factor joint exposure apparatus for sea cucumbers according to claim 1, wherein: a pH probe (29) and a dissolved oxygen probe (30) are arranged in the water distribution tank (7).
CN202011069877.8A 2020-10-09 2020-10-09 Multi-environmental factor joint exposure device suitable for sea cucumbers Pending CN112162074A (en)

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Application Number Priority Date Filing Date Title
CN202011069877.8A CN112162074A (en) 2020-10-09 2020-10-09 Multi-environmental factor joint exposure device suitable for sea cucumbers

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Application Number Priority Date Filing Date Title
CN202011069877.8A CN112162074A (en) 2020-10-09 2020-10-09 Multi-environmental factor joint exposure device suitable for sea cucumbers

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CN112162074A true CN112162074A (en) 2021-01-01

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113092061A (en) * 2021-04-07 2021-07-09 大连理工大学 Water tank active absorption type wave generator system and control method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113092061A (en) * 2021-04-07 2021-07-09 大连理工大学 Water tank active absorption type wave generator system and control method

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