CN112138141B - Oral pharmaceutical composition of somatostatin or analogue thereof - Google Patents
Oral pharmaceutical composition of somatostatin or analogue thereof Download PDFInfo
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- CN112138141B CN112138141B CN201910498794.1A CN201910498794A CN112138141B CN 112138141 B CN112138141 B CN 112138141B CN 201910498794 A CN201910498794 A CN 201910498794A CN 112138141 B CN112138141 B CN 112138141B
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- somatostatin
- pharmaceutical composition
- small intestine
- test
- absorption
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- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009747 swallowing Effects 0.000 description 1
- 230000002889 sympathetic effect Effects 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
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- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/10—Peptides having 12 to 20 amino acids
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- A61K38/00—Medicinal preparations containing peptides
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- A61K38/08—Peptides having 5 to 11 amino acids
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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Abstract
The invention belongs to the field of biological medicine, and in particular relates to an oral pharmaceutical composition of somatostatin or analogues thereof, which comprises the following components: the somatostatin and/or somatostatin analogues and the small intestine absorption promoting pharmaceutical composition are prepared, wherein the small intestine absorption promoting pharmaceutical composition consists of sodium dodecyl sulfate, carbomer, chitosan and sodium citrate; the pharmaceutical composition for promoting the absorption of the small intestine is prepared into a composite auxiliary material, and the auxiliary material and the somatostatin and/or the somatostatin analogue composition can improve the absorption of the active ingredient in the small intestine and the like.
Description
Technical Field
The invention belongs to the technical field of biological medicines, and particularly relates to an oral pharmaceutical composition of somatostatin or an analogue thereof.
Background
Somatostatin (growth hormone release-inlerase-inhibiting hormone, GHRIH, or somatastatin) is a tetradecapeptide cleaved from a macromolecular peptide of 116 amino acids, which is cyclic in structure, with a disulfide bond between cysteines at positions 3 and 14; somatostatin is a neurohormone with a relatively wide range of actions, and its main action is to inhibit basal secretion of pituitary Growth Hormone (GH), and also inhibit the response of pituitary gland to GH secretion caused by various stimuli, including exercise, meal, stress, hypoglycemia, etc. In addition, somatostatin can also inhibit LH, FSH, TSH, PRL secretion of ACTH. Somatostatin acts by reducing intracellular cAMP and Ca2 after binding to membrane receptors on pituitary auxin cells. In addition to the hypothalamus, somatostatin is widely present in other areas such as the cerebral cortex, striatum, amygdala, hippocampus, spinal cord, sympathetic nerves, gastrointestinal tract, islets, kidneys, thyroid gland and parathyroid gland. The GHRIH28 composed of 28 amino acids is purified in brain and stomach, and the N end of GHRIH14 extends outwards. The extrapituitary effect of somatostatin is relatively complex and it may act as a transmitter or modulator in the nervous system; somatostatin has certain inhibition effect on gastrointestinal motility and secretion of digestive tract hormone; it also inhibits secretion of insulin, glucagon, renin, parathyroid hormone, and calcitonin.
None of somatostatin and its analogues can be orally administered, which results in poor patient compliance, and therefore, it is important to alter the route of administration of somatostatin and its analogues.
Disclosure of Invention
Based on the reasons, the applicant obtains a novel pharmaceutical composition for promoting small intestine absorption, which consists of sodium dodecyl sulfate, carbomer, chitosan and sodium citrate through a plurality of creative researches, and the research shows that the pharmaceutical composition for promoting small intestine absorption has the effect of preparing a composite auxiliary material, wherein the auxiliary material and the somatostatin and/or the somatostatin analogue composition can improve the absorption of the active ingredient in small intestine.
The invention is realized by the following technical scheme.
A pharmaceutical composition comprising: the somatostatin and/or somatostatin analogues and the small intestine absorption promoting pharmaceutical composition are prepared, wherein the small intestine absorption promoting pharmaceutical composition consists of sodium dodecyl sulfate, carbomer, chitosan and sodium citrate.
The pharmaceutical composition is prepared into an oral preparation.
Somatostatin analogs include: octreotide.
The pharmaceutical composition for promoting small intestine absorption is used for guaranteeing the absorption of somatostatin and/or somatostatin analogues in small intestine.
The pharmaceutical composition for promoting intestinal absorption is used for promoting the absorption of somatostatin and/or somatostatin analogues in small intestines.
Wherein the weight ratio of the sodium dodecyl sulfate to the carbomer to the chitosan to the sodium citrate is 15-25:5-8:5-8:50-80.
Wherein the weight ratio of the somatostatin and/or the somatostatin analogue to the small intestine absorption promoting pharmaceutical composition is as follows: 1:5-860.
An oral preparation is prepared from somatostatin and/or somatostatin analogues, sodium dodecyl sulfate, carbomer, chitosan and sodium citrate.
Wherein the weight ratio of the sodium dodecyl sulfate to the carbomer to the chitosan to the sodium citrate is 15-25:5-8:5-8:50-80.
Wherein the weight ratio of the somatostatin and/or the somatostatin analogue to the small intestine absorption promoting pharmaceutical composition is as follows: 1:5-860.
The small intestine absorption promoting pharmaceutical composition of the invention obtains a novel auxiliary material which can be used for: drugs (active ingredients or active ingredients) which cannot be orally administered but can be injected only can be orally administered, thereby changing the administration mode of the drugs (active ingredients or active ingredients).
The small intestine absorption promoting pharmaceutical composition of the present invention can promote the absorption of a drug (active ingredient or active ingredient) which is easily decomposed in the gastrointestinal tract in the intestine.
The small intestine absorption promoting pharmaceutical composition of the present invention can promote the absorption of a drug (active ingredient or active ingredient) which is not easily absorbed in the gastrointestinal tract in the intestine.
Because the pharmaceutical composition for promoting small intestine absorption of the invention promotes the absorption of medicines (active ingredients or active ingredients) in small intestine and requires release in small intestine to exert the efficacy, rodents are administrated by small intestine catheters and mammals are administrated orally by enteric capsules when pharmacodynamic tests and drug substitution tests are carried out.
The invention matches the pharmaceutical composition for promoting intestinal absorption and the medicine (active ingredient or active ingredient) one by one on rodents for bioavailability detection, and simultaneously selects part of polypeptides for detecting the drug effect and pharmacokinetics on different animals.
Drawings
1. FIG. 1 shows PD test of Exenatide on STZ rats
Wherein: the abscissa is time (h), and the ordinate is the blood glucose lowering efficiency (%).
Wherein: the solid circular solid line is 2ml/kg of physiological saline injected into the small intestine, the solid square dashed line is 1 μg/kg of subcutaneous Exenatide, the solid circular dashed line is 250 μg/kg of subcutaneous Exenatide, the solid triangular dashed line is 1mg/kg of subcutaneous Exenatide, the solid triangular solid line is 30 μg/kg of pharmaceutical composition +Exenatide for small intestine administration test 1, the solid hollow circular solid line is 40 μg/kg of pharmaceutical composition +Exenatide for small intestine administration test 1, the solid hollow square line is 50 μg/kg of pharmaceutical composition +Exenatide for small intestine administration test 1, and the solid hollow diamond line is 60 μg/kg of pharmaceutical composition +Exenatide for small intestine administration test 1.
2. FIG. 2 shows the iv PK test of Exenatide on rats
Wherein: the abscissa is time (min) and the ordinate is Exenatide concentration (ng/ml) in rat plasma.
3. FIG. 3 shows the ei PK assay of Exenatide/test 1 pharmaceutical compositions on rats
Wherein: the abscissa is time (min) and the ordinate is Exenatide concentration (ng/ml) in rat plasma.
4. FIG. 4 is an iv PK test of Exenatide on beagle dogs
Wherein: the abscissa is time (min) and the ordinate is Exenatide concentration (ng/ml) in beagle plasma.
5. FIG. 5 is a po PK test of Exenatide/test 1 pharmaceutical compositions on beagle dogs
Wherein: the abscissa is time (min) and the ordinate is Exenatide concentration (ng/ml) in beagle plasma.
6. FIG. 6 is a PD test on an Alloxan beagle by Exenatide
Wherein: the abscissa is time (h) and the ordinate is beagle glucose (mM).
Wherein: solid circular solid lines are postprandial glycemic profiles for Alloxan beagle dogs, solid square solid lines are postprandial glycemic profiles for Alloxan beagle dogs following swallowing of Exenatide/test 1 pharmaceutical composition, and solid diamond solid lines are postprandial serum profiles for normal beagle dogs.
Specific test examples
The following will explain the embodiments of the present invention by way of specific examples, but the scope of the present invention is not limited thereto.
The description of the test examples in this specification is merely an illustration of implementation forms of the inventive concept and the scope of protection of the invention should not be construed as being limited to the specific forms set forth in the test examples, as well as equivalent technical means which can be conceived by those skilled in the art based on the inventive concept. Although the following embodiments of the present invention are described, the present invention is not limited to the specific embodiments and fields of application described above, which are illustrative, instructive, and not limiting. Those skilled in the art, having the benefit of this disclosure, may effect numerous forms of the invention without departing from the scope of the invention as claimed.
The following test is a conclusion test of summarized research and development personnel based on the technical scheme to be protected by the invention on the basis of multiple creative tests. In the following test examples, three repeated experiments were set, and the data are the average value or the average value.+ -. Standard deviation of the three repeated experiments.
Test 1 significantly improves the efficacy of small intestine administered Exenatide (Exendin 4, EXE 4)
The pharmaceutical composition for promoting intestinal absorption comprises the following components: sodium dodecyl sulfate, carbomer, chitosan and sodium citrate in the weight ratio: 20:6.5:6.5:65.
fully and uniformly mixing Exenatide and the medicinal composition according to the weight ratio of 1:5 for later use;
test animals: SD male rats were intraperitoneally injected with 45mg/kg STZ to construct a hyperglycemic model;
small intestine drug efficacy test: blood samples were taken at 0h,3h,6h and 9h for blood glucose testing either subcutaneously (sc) or via small intestine catheter (ei).
The results show that the Exenatide administered into the small intestine has weak hypoglycemic effect under the condition that the pharmaceutical composition is not added, and the hypoglycemic efficiency after 9 hours is only about 70 percent when the dosage reaches 1mg/kg, which is far lower than about 50 percent of the dosage which can reach 1 mug/kg subcutaneously. And after the pharmaceutical composition is added, the subcutaneous blood glucose reducing effect of 1 mug/kg can be achieved by the administration dosage of 50 mug/kg. See fig. 1.
Test 2 significantly improves the bioavailability of Exenatide administered to the small intestine
Fully and uniformly mixing Exenatide and the test 1 small intestine absorption promoting pharmaceutical composition according to the weight ratio of 1:5 for later use;
test animals: adult male SD rats;
small intestine PK assay: on adult SD rats in the fasting state, the administration was carried out through a small intestine catheter at a dose of 200. Mu.g/kg by a dose volume of 1ml/kg, and the other group was injected (ei) with 200. Mu.g/kg of Exenatide or Exenatide added with the pharmaceutical composition of the present invention by a small intestine catheter, 0h,0.5h,1h,1.5h,2h,2.5h and 3h after administration, blood was collected from the tail, blood was anticoagulated with 10mM EDTA, centrifuged at 3000rpm at 4℃for 5min, and plasma quick-freezing was collected.
To avoid hypoglycemia in the animals, 1g/kg of glucose was administered prior to administration.
ELISA detection method: coating with anti-target polypeptide mouse monoclonal antibody, blocking with 1% BSA, adding blood sample or 0.1% BSA diluted standard substance for incubation, capturing Biotin labeled anti-target polypeptide rabbit polyclonal antibody, incubating HRP coupled streptavidine, finally TMB developing, HCl stopping, and reading at 450 nm. And calculating the concentration of the target polypeptide in the blood plasma according to a standard curve obtained by the standard substance.
AUC was calculated from PK profile and bioavailability for small intestine administration was calculated as 100% of bioavailability for intravenous injection (iv).
The results showed that the AUC of the PK profile after iv injection of Exenatide at 1. Mu.g/kg was 0.93ng/ml.h, and that the blood concentration had been below the ELISA lower limit of detection after small intestine injection at 200. Mu.g/kg. Whereas the AUC of the PK profile after addition of the test 1 pharmaceutical composition could reach 1.47ng/ml.h, the bioavailability for small intestine administration was about 0.79%. The test results are shown in fig. 2 and 3.
Test 3 significantly improves the bioavailability of oral Exenatide
Mixing Exenatide 0.7mg and test 1 small intestine absorption promoting pharmaceutical composition 200mg thoroughly, lyophilizing, and making into No. 3 enteric capsule;
mixing Exenatide 0.7mg with test 1 small intestine absorption promoting pharmaceutical composition 400mg, lyophilizing, and making into No. 0 enteric capsule;
mixing Exenatide 0.7mg and test 1 pharmaceutical composition for promoting intestinal absorption 600mg thoroughly, lyophilizing, and making into No. 00 enteric capsule;
mixing Exenatide 0.7mg and test 1 small intestine absorption promoting pharmaceutical composition 200mg thoroughly, lyophilizing, and making into common capsule No. 3;
mixing Exenatide 0.7mg and mannitol 200mg thoroughly, lyophilizing, and making into enteric capsule No. 3;
test animals: adult male beagle dogs
Oral PK assay: blood samples were collected at 0.5,1,1.5,2,2.5,3h after oral administration of the enteric capsule in the fasting state of the animal. Blood samples were anticoagulated with 10mM EDTA, centrifuged at 3000rpm for 5min at 4℃and plasma flash frozen.
Venous PK test: the animals were given a fasting state and blood samples were collected by intravenous injection of 0.3 μg/kg Exenatide at 5, 15, 30, 60, 90, 120 min. Blood samples were anticoagulated with 10mM EDTA, centrifuged at 3000rpm for 5min at 4℃and plasma flash frozen. See fig. 4 and 5.
To avoid hypoglycemia in the animals, 1g/kg of glucose was administered prior to administration.
ELISA detection method: coating with anti-target polypeptide mouse monoclonal antibody, blocking with 1% BSA, adding blood sample or 0.1% BSA diluted standard substance for incubation, capturing Biotin labeled anti-target polypeptide rabbit polyclonal antibody, incubating HRP coupled streptavidine, finally TMB developing, HCl stopping, and reading at 450 nm. And calculating the concentration of the target polypeptide in the blood plasma according to a standard curve obtained by the standard substance.
AUC was calculated from PK profile and bioavailability for small intestine administration was calculated as 100% of bioavailability for intravenous injection (iv).
The PK data for beagle dogs showed that the AUC for intravenous injection of 0.3 μg/kg Exenatide was about 0.82ng/ml. Hour, and the AUC for oral Exenatide/test 1 pharmaceutical composition 0.7mg was about 1.36ng/ml. Hour. The bioavailability of the oral Exenatide/test 1 pharmaceutical composition was about 0.83%.
Exenatide cannot successfully enter blood without the assistance of the pharmaceutical composition, and blood entering efficiency is remarkably improved after the pharmaceutical composition is added. Although Exenatide blood entry efficiency increased slightly with increasing weight of the test 1 pharmaceutical composition, the magnitude of the increase was limited. The capsule number 3 is suitable for taking the convenience of oral administration and the effectiveness of the medicine into consideration.
Table 1 Exenatide/test 1 po PD test of pharmaceutical compositions for promoting intestinal absorption on beagle dogs
Test 4 Exenatide/test 1 intestinal absorption promoting pharmaceutical composition can obviously inhibit the postprandial blood glucose increase of Alloxan beagle
Mixing Exenatide 0.7mg and test 1 small intestine absorption promoting pharmaceutical composition 200mg thoroughly, lyophilizing, and making into No. 3 enteric capsule;
test animals: adult male beagle dogs;
physical examination and adaptation of animals: collecting an animal fasting blood sample, detecting blood biochemical indexes, and after determining that all the indexes are normal, placing the animal in a quieter room for 1 week, wherein the daily feeding time and the feeding amount are required to be consistent;
data acquisition before molding: blood samples were collected at 4 time points per day (2 h, 4h, 6h before feeding, after feeding) for 5 days continuously;
and (3) molding test: in a fasting state, 60mg/kg of Alloxan solution is injected intravenously, blood samples are collected at 4 time points (2 h, 4h and 6h before feeding and after feeding) every day after one week, and continuous collection is carried out for 5 days; and judging whether the model is qualified or not according to the acquired data. If the test is qualified, starting a drug effect test;
efficacy test: the test capsules were swallowed before feeding and blood samples were taken at 4 time points (2 h, 4h, 6h before feeding, after feeding).
The results show that Exenatide/test 1 intestinal absorption-promoting pharmaceutical composition can significantly inhibit postprandial blood glucose elevation in Alloxan-molded beagle dogs. See fig. 6.
Conclusion of the test: the test shows that the medicine composition for promoting the intestinal absorption has good effect of promoting the absorption of the effective components which cannot be orally taken in the intestines, and can be used as a novel medicinal auxiliary material.
Test example 5 the pharmaceutical composition of the present invention can significantly improve the bioavailability of Octreotide (Octreotide) administered into the small intestine
The medicine composition comprises the following components: the weight ratio of the sodium dodecyl sulfate, carbomer, chitosan and sodium citrate is as follows: 21:7:6:69.
octreotide and the pharmaceutical composition of the invention are mixed according to the weight ratio of 1:5, fully and uniformly mixing for later use;
test animals: adult male SD rats;
small intestine PK assay: on adult SD rats in the fasting state, octreotide was administered via a small intestine catheter at a dose of 1ml/kg, 200. Mu.g/kg of octreotide was added to the pharmaceutical composition of the present invention by small intestine catheter injection (ei) in another group, 0h,0.5h,1h,1.5h,2h,2.5h and 3h after administration, tail blood sampling, anticoagulation of blood sample by 10mM EDTA, centrifugation at 3000rpm for 5min at 4℃and plasma quick freezing were collected.
Venous PK test: animals were given 1 μg/kg octreotide intravenously in a fasting state and blood samples were collected at 5, 15, 30, 60, 90, 120 min. Blood samples were anticoagulated with 10mM EDTA, centrifuged at 3000rpm for 5min at 4℃and plasma flash frozen.
ELISA detection method comprises coating with mouse monoclonal antibody against target polypeptide, blocking with 1% BSA, adding blood sample or 0.1% BSA diluted standard substance for incubation, capturing rabbit polyclonal antibody against target polypeptide labeled by Biotin, incubating HRP coupled strepavidin, and finally TMB developing, terminating with HCl, and reading at 450 nm. And calculating the concentration of the target polypeptide in the blood plasma according to a standard curve obtained by the standard substance.
AUC was calculated from PK profile and bioavailability for small intestine administration was calculated as 100% of bioavailability for intravenous injection (iv).
The results show that octreotide is administered through the small intestine at 200 μg/kg, and the blood concentration is below the ELISA detection limit. And after the pharmaceutical composition is added, the bioavailability of the small intestine administration can reach 1.71 percent.
Experiment 6 the pharmaceutical composition for promoting intestinal absorption of the present invention can significantly improve the bioavailability of somatostatin-14 administered into the small intestine
Pharmaceutical composition for promoting intestinal absorption: the weight ratio of the sodium dodecyl sulfate, carbomer, chitosan and sodium citrate is as follows: 20:6.5:6.5:68.
fully and uniformly mixing the somatostatin-14 with the pharmaceutical composition according to the weight ratio of 1:5 for later use;
test animals: adult male SD rats;
small intestine PK assay: on adult SD rats in the fasting state, 1ml/kg of the administration volume was administered through a small intestine catheter to make the dose of somatostatin-14 200. Mu.g/kg, and another group of the small intestine catheters was injected (ei) with 200. Mu.g/kg of somatostatin-14 or with 200. Mu.g/kg of somatostatin-14 added to the pharmaceutical composition for promoting small intestine absorption of the present invention, 0h,0.5h,1h,1.5h,2h,2.5h and 3h after administration, tail blood was collected, blood samples were anticoagulated with 10mM EDTA, centrifuged at 4℃and 3000rpm for 5min, and plasma quick-freezing was collected.
Venous PK test: the animals were on empty stomach and blood samples were collected by intravenous injection of 1. Mu.g/kg somatostatin-14 at 5, 15, 30, 60, 90, 120 min. Blood samples were anticoagulated with 10mM EDTA, centrifuged at 3000rpm for 5min at 4℃and plasma flash frozen.
ELISA detection method comprises coating with mouse monoclonal antibody against target polypeptide, blocking with 1% BSA, adding blood sample or 0.1% BSA diluted standard substance for incubation, capturing rabbit polyclonal antibody against target polypeptide labeled by Biotin, incubating HRP coupled strepavidin, and finally TMB developing, terminating with HCl, and reading at 450 nm. And calculating the concentration of the target polypeptide in the blood plasma according to a standard curve obtained by the standard substance.
AUC was calculated from PK profile and bioavailability for small intestine administration was calculated as 100% of bioavailability for intravenous injection (iv).
The results showed that somatostatin-14 was injected into the small intestine at 200 μg/kg with blood concentration below ELISA detection limit. And after the pharmaceutical composition for promoting the absorption of the small intestine is added, the bioavailability of the somatostatin-14 small intestine can reach 0.70 percent.
Experiment 7 the pharmaceutical composition for promoting intestinal absorption of the present invention can significantly improve the bioavailability of somatostatin-28
The invention relates to a pharmaceutical composition for promoting intestinal absorption, which comprises the following components: the weight ratio of the sodium dodecyl sulfate, carbomer, chitosan and sodium citrate is as follows: 3:1:1:10.
fully and uniformly mixing somatostatin-28 with the small intestine absorption promoting pharmaceutical composition according to the weight ratio of 1:5 for later use;
test animals: adult male SD rats;
small intestine PK assay: on adult SD rats in the fasting state, 1ml/kg of the administration volume was administered through a small intestine catheter to make the dose of somatostatin-28 200. Mu.g/kg, and another group of the small intestine catheters was injected (ei) with 200. Mu.g/kg of somatostatin-28 or with the addition of somatostatin-28 of the pharmaceutical composition for promoting intestinal absorption of the present invention, 0h,0.5h,1h,1.5h,2h,2.5h and 3h after administration, blood was collected from the tail, blood was anticoagulated with 10mM EDTA, centrifuged at 4℃and 3000rpm for 5min, and plasma quick-freezing was collected.
Venous PK test: the animals were on empty stomach and blood samples were collected by intravenous injection of 1 μg/kg somatostatin-28 at 5, 15, 30, 60, 90, 120 min. Blood samples were anticoagulated with 10mM EDTA, centrifuged at 3000rpm for 5min at 4℃and plasma flash frozen.
ELISA detection method comprises coating with mouse monoclonal antibody against target polypeptide, blocking with 1% BSA, adding blood sample or 0.1% BSA diluted standard substance for incubation, capturing rabbit polyclonal antibody against target polypeptide labeled by Biotin, incubating HRP coupled strepavidin, and finally TMB developing, terminating with HCl, and reading at 450 nm. And calculating the concentration of the target polypeptide in the blood plasma according to a standard curve obtained by the standard substance.
AUC was calculated from PK profile and bioavailability for small intestine administration was calculated as 100% of bioavailability for intravenous injection (iv).
The results showed that somatostatin-28 was injected into the small intestine at 200 μg/kg with blood concentration below ELISA detection limit. And after the pharmaceutical composition for promoting the absorption of the small intestine is added, the bioavailability of the somatostatin-28 small intestine can reach 0.40 percent.
Claims (3)
1. The pharmaceutical composition is characterized by being prepared from somatostatin and/or somatostatin analogues and a small intestine absorption promoting pharmaceutical composition, wherein the small intestine absorption promoting pharmaceutical composition consists of sodium dodecyl sulfate, carbomer, chitosan and sodium citrate; wherein the weight ratio of the sodium dodecyl sulfate to the carbomer to the chitosan to the sodium citrate is 15-25:5-8:5-8:50-80 parts; the medicine composition is prepared into oral preparation; wherein the weight ratio of the somatostatin and/or the somatostatin analogue to the small intestine absorption promoting pharmaceutical composition is as follows: 1:5-860; wherein the somatostatin analogue is: octreotide.
2. A pharmaceutical composition according to claim 1 for use in ensuring absorption of somatostatin and/or somatostatin analogues in the small intestine.
3. A pharmaceutical composition according to claim 1 for use in promoting absorption of somatostatin and/or somatostatin analogues in the small intestine.
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| 口服吸收促进剂研究进展概述;金朝辉等;《华西医学》;20081231;第23卷(第4期);第940页中栏第3段、第941页中栏第3段 * |
| 胰岛素口服给药途径的研究进展;刘利等;《现代食品与食品杂志》;20171231;第17卷(第6期);第62页右栏倒数第2段 * |
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